Constant-temperature and constant-humidity test box for cultivating strains

Abstract

The invention relates to the technical field of strain cultivation, in particular to a constant-temperature and constant-humidity test box for cultivating strains, which comprises a box body, a window is arranged on the box body, a temperature and humidity control system is arranged in the box body, an advancing conveying mechanism is further arranged in the box body, and the advancing conveying mechanism comprises a conveying supporting structure. A first liquid adding mechanism, a second liquid adding mechanism, a stirring mechanism and a liquid pumping mechanism are sequentially arranged on the conveying supporting structure along the way; the culture box enters the box body through the window to culture bacteria, and when passing through the window, the cleaning mechanism works to prevent more external bacteria from entering the box body to influence the culture environment; then the liquid sequentially passes through a first liquid adding mechanism and a second liquid adding mechanism; after liquid injection is completed, the sealing module can return into the liquid extraction opening under the elastic effect, the large elastic force of the sealing module can drive the side ring block to be tightly attached to the sealing column, the top check block located on the top of the side ring block just shields the liquid extraction hole, liquid injection cannot be conducted at the moment, the sealing performance of the incubator is guaranteed, and the quality of the cultivation environment is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial strain cultivation, and in particular to a constant temperature and humidity test box for cultivating bacterial strains. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro so that cells can survive, grow, reproduce and maintain their main structure and function. As the main carrier of cell culture, cell culture equipment can simulate the external environment suitable for cell growth, and is therefore widely used in the field of cell culture.

[0003] Among them, the invention with application number CN202111553238.3 discloses an animal cell culture drying test box, which relates to the field of test box technology, and solves the technical problem that the current test boxes all use natural ventilation to dry, have low drying efficiency and speed, and have poor sealing, which makes it easy for fine dust to enter the interior and affect cell culture work; it includes a box body, a support column is fixedly connected to the bottom of the inner cavity of the box body, a placement table is fixedly connected to the top of the support column, a culture dish is placed on the top of the placement table, four correspondingly distributed heating plates are arranged at the bottom of the inner cavity of the box body, a sealing mechanism is arranged on the outside of the box body, and a drying mechanism is arranged on the inside of the box body; the inside of the test box can be automatically and quickly dried and external dust can be prevented from entering the test box.

[0004] Existing test chambers cultivate bacteria through culture dishes. Since cultivating bacterial cells requires a harsh environment, when most culture dishes are placed in the test chamber for cultivation, external bacteria are easily brought into the chamber. After entering the chamber, these external bacteria will multiply in the chamber and affect the bacterial cultivation in the culture dishes. Summary of the invention

[0005] The purpose of the present invention is to provide a constant temperature and humidity test box for cultivating bacteria in view of the shortcomings of the prior art.

[0006] To achieve the above object, the technical solution of the present invention is as follows:

[0007] A constant temperature and humidity test box for cultivating bacteria comprises a box body, the box body is provided with a viewing window for entering the incubator, and a temperature and humidity control system is arranged inside the box body.

[0008] The viewing window is provided with a cleaning mechanism for removing dust from the surface of the incubator, the cleaning mechanism comprising a guide rail structure coaxially arranged outside the viewing window, a cleaning sliding block slidably mounted on the guide rail structure, a cleaning sliding seat connected to a cleaning driving seat, a rotatable brush member mounted on the cleaning driving seat; a driving chain is arranged along the moving track of the cleaning sliding block on the guide rail structure, and a transmission seat movably connected to the cleaning sliding block is mounted on the driving chain;

[0009] The box body is also provided with a forward conveying mechanism for conveying the incubator, the forward conveying mechanism includes a conveying support structure, and the conveying support structure is sequentially arranged with a first liquid adding mechanism, a second liquid adding mechanism, a stirring mechanism and a liquid pumping mechanism along the way;

[0010] The conveying support structure comprises a supporting conveyor belt, and side support bars for sliding and positioning the side of the incubator are respectively arranged on both sides of the supporting conveyor belt in the forward direction, and support rollers are installed on the top of the side support bars along the length direction; a supporting conveyor frame is arranged at the bottom of the supporting conveyor belt, and the first liquid adding mechanism, the second liquid adding mechanism and the liquid pumping mechanism are respectively installed on the supporting conveyor frame;

[0011] The liquid extraction mechanism includes a liquid extraction rod that can move longitudinally; a liquid extraction port is opened on the top of the incubator, and a sealing module is installed on the liquid extraction port. The sealing module includes a sealing column that is elastically inserted into the liquid extraction port, and the sealing column is formed with a liquid extraction hole with a hollow structure, and the liquid extraction hole can be inserted into the liquid extraction rod. The outer ring part of the sealing column is annular and has multiple side ring blocks with arc structures arranged at equal intervals. An elastic structure for elastically popping out the side ring block is arranged between the side ring block and the sealing column; a top stopper for blocking the liquid extraction hole is formed on the top of the side ring block.

[0012] The beneficial effects of the present invention are as follows: the culture box filled with bacteria enters the box through the viewing window to cultivate the bacteria; when passing through the viewing window, the cleaning mechanism works, the driving chain moves under the drive of the sprocket, and the cleaning sliding block moves one circle along the guide rail structure under the drive of the transmission seat, and the brush member installed on the cleaning driving seat can clean the outer wall of the box, thereby preventing more external bacteria from entering the box and affecting the culture environment;

[0013] During liquid injection, the sealing module located at the liquid extraction port will work, and a portion of the sealing column will pop outward under the action of elasticity. At the same time, the side ring block on the outer ring of the sealing column will radially spring open under the action of the elastic structure, and the top block on the top of the side ring block cannot block the liquid extraction hole. At this time, the liquid injection head can be lowered and inserted into the liquid extraction hole to realize the liquid addition operation, and the liquid enters the incubator through the sealing module of the liquid extraction port. When the liquid injection is completed, the sealing module will retreat to the liquid extraction port under the action of elasticity, and its larger elastic force can drive the side ring block to be close to the sealing column, and the top block on the top of the side ring block just blocks the liquid extraction hole. At this time, liquid cannot be injected, ensuring the sealing of the incubator and the quality of the cultivation environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of the box.

[0015] Figure 2 A schematic diagram of the structure of the viewing window.

[0016] Figure 3 It is a schematic diagram of the structure of the cleaning mechanism.

[0017] Figure 4 It is a structural schematic diagram of the humidification component.

[0018] Figure 5 It is a structural schematic diagram of the conveying support structure.

[0019] Figure 6 It is a structural schematic diagram of the cooperation between the first liquid adding mechanism and the incubator.

[0020] Figure 7 It is a structural schematic diagram of the coordination between the conveying support structure and the incubator.

[0021] Figure 8 It is a structural schematic diagram of the first liquid adding mechanism or the second liquid adding mechanism.

[0022] Fig. 9 It is a structural schematic diagram of the liquid injection module.

[0023] Fig.10 A partial cross-sectional schematic diagram of the incubator.

[0024] Fig.11 for Fig.10 A partial enlarged cross-sectional schematic diagram of .

[0025] Fig.12 This is a schematic diagram of the structure of the stirring robot and the incubator.

[0026] Reference numerals include:

[0027] 1-Box,

[0028] 11-View window,

[0029] 111-external guide window, 112-internal guide window, 113-guide linear module, 114-universal shaft,

[0030] 115-exhaust outlet, 116-temperature and humidity control system, 117-humidifier, 118-air conditioner,

[0031] 12- Cleaning agency,

[0032] 121-guide rail structure, 122-cleaning sliding block, 123-cleaning driving seat, 124-brush member,

[0033] 125-driving chain, 126-transmission seat, 127-cleaning support bar,

[0034] 13-Humidification component,

[0035] 131-top humidification installation strip, 132-side humidification installation strip, 133-bottom humidification installation strip,

[0036] 134-humidification nozzle, 135-support hole, 136-support column, 137-humidification support plate,

[0037] 2-Conveying support structure,

[0038] 21-Support conveyor belt,

[0039] 211-side support bar, 212-support roller, 213-support conveyor frame, 214-unloading conveyor belt,

[0040] 22- first liquid adding mechanism,

[0041] 220-first liquid adding support column, 221-liquid adding plate, 222-extrusion module, 223-liquid injection piston mechanism, 224-extrusion liquid inlet, 225-extrusion liquid outlet, 226-liquid supply pipe, 227-extrusion linear module,

[0042] 228-longitudinal moving plate, 229-longitudinal piston rod, 2291-sealing seat,

[0043] 23-Liquid injection module,

[0044] 231-liquid injection plate, 232-liquid injection head, 233-liquid injection lifting cylinder, 234-liquid injection horizontal plate,

[0045] 235-leakage-proof plate, 236-leakage-proof guide rod, 237-leakage-proof guide hole, 238-liquid injection guide plate,

[0046] 239-arc groove, 2391-leakage-proof roller,

[0047] 3-Incubator,

[0048] 31-hole structure,

[0049] 311-first downward pressure hole, 312-second downward pressure hole, 313-U-shaped hole, 314-first movable rod,

[0050] 315-second movable block, 316-U-shaped connecting strip, 317-first compression spring,

[0051] 318-inner movable hole, 319-stop block,

[0052] 32- Sealing module,

[0053] 321-liquid extraction port, 322-limiting port, 323-sealing column, 324-liquid extraction hole, 325-side ring block,

[0054] 326-connecting ring, 327-second compression spring, 328-top stopper,

[0055] 4-Stirring mechanism,

[0056] 40-mixing robot, 41-executing end, 42-suction cup module, 43-suction cup fixing seat,

[0057] 44-suction cup frame, 45-suction cup piece,

[0058] 46-downward pressure assembly, 47-downward pressure block, 50-second liquid adding mechanism, 60-liquid extraction mechanism,

[0059] 61-liquid extraction support plate, 62-liquid extraction rod. DETAILED DESCRIPTION

[0060] The present invention is described in detail below with reference to the accompanying drawings.

[0061] like Figure 1-12 As shown, a constant temperature and humidity test box for cultivating bacterial strains includes a box body 1, the box body 1 is provided with a viewing window 11 for the incubator 3 to enter, and a temperature and humidity control system 116 is provided in the box body 1. The temperature and humidity control system 116 includes a humidifier 117 and an air conditioner 118, both of which are arranged outside the box body 1, and a pipe is provided at the outlet of the humidifier 117 to connect to the box body 1; a pipe is provided at the outlet of the air conditioner 118 to connect to the box body 1, and a temperature sensor and a humidity sensor are provided in the box body 1. Through the cooperation of the humidifier 117, the air conditioner 118, the temperature sensor and the humidity sensor, the humidity and temperature inside the box body 1 can be kept stable, and can be kept constant in a relatively uniform environment, forming a constant temperature and humidity effect, which is convenient for the cultivation of bacteria and has a better quality of cultivation.

[0062] The box body 1 is also provided with a forward conveying mechanism for conveying the incubator 3, and the forward conveying mechanism includes a conveying support structure 2, and the conveying support structure 2 is sequentially arranged with a first liquid adding mechanism 22, a second liquid adding mechanism 50, a stirring mechanism 4 and a liquid pumping mechanism 60 along the way.

[0063] The viewing window 11 is provided with a cleaning mechanism 12 for removing dust from the surface of the incubator 3. The cleaning mechanism 12 includes a guide rail structure 121 coaxially arranged outside the viewing window 11. A cleaning sliding block 122 is slidably mounted on the guide rail structure 121. The cleaning sliding seat is connected to a cleaning drive seat 123. The cleaning drive seat 123 is equipped with a rotatable brush member 124. The guide rail structure 121 is provided with a driving chain 125 along the moving track of the cleaning sliding block 122. The driving chain 125 is equipped with a brush member 124 that is connected to the cleaning sliding block 122. 2 movably connected transmission seat 126; the culture box 3 filled with bacteria enters the box body 1 through the viewing window 11 to cultivate bacteria; when passing through the viewing window 11, the cleaning mechanism 12 works, the driving chain 125 moves under the drive of the sprocket, and driven by the transmission seat 126, the cleaning sliding block 122 moves one circle along the guide rail structure 121, and the brush member 124 installed on the cleaning driving seat 123 can clean the outer wall of the box body 1 to prevent more external bacteria from entering the box body 1 and affecting the culture environment.

[0064] Preferably, the number of the brush pieces 124 is more than four, and two adjacent brush pieces 124 are arranged equidistantly, and each brush piece 124 is slidably connected to the guide rail structure 121 through the cleaning sliding block 122, and the drive chain 125 is provided with a corresponding number of transmission seats 126 connected to the cleaning sliding block 122, and there is a certain gap between the drive chain 125 and the guide rail structure 121, and the sprocket can be installed at the gap through the shaft, and four motors are arranged equidistantly in the gap, and rotate under the drive of the motor, thereby driving the drive chain 125 to move. Adopting the above scheme, when cleaning the outer wall of the incubator 3, the efficiency is faster. In addition, in order to improve the efficiency of cleaning, the cleaning mechanism 12 also includes a cleaning support bar 127 arranged vertically along the forward direction of the culture, and the cleaning support bar 127 is driven by a lifting cylinder. When cleaning, the cleaning support bar 127 can lift the incubator 3, so that the incubator 3 has a gap with the bottom, and at this time, the brush piece 124 can pass through, and the bottom wall of the incubator 3 can be completely cleaned.

[0065] Preferably, linear modules can be arranged in the vertical direction and the horizontal direction respectively. The driving end of the linear module is connected to the cleaning sliding block 122 through the transmission seat 126. The cleaning sliding block 122 can move along the length direction of the guide track. The cleaning sliding blocks 122 located at the top and the bottom can move horizontally to clean the outer wall of the incubator 3; the cleaning sliding blocks 122 located at the side can move up and down to clean the outer wall of the incubator 3.

[0066] Preferably, an outer guide window 111 is installed on the outer side of the viewing window 11, and an inner guide window 112 is installed on the inner side of the viewing window 11, and the inner guide window 112 is connected to the support conveyor belt 21 of the cleaning mechanism 12; the cleaning mechanism 12 is arranged along the area along the outer guide window 111; a guide linear module 113 is arranged between the outer guide window 111 and the inner guide window 112 along the length direction, and a universal shaft 114 movably connected to the incubator 3 is installed on the driving end of the guide linear module 113. Under the drive of the guide linear module 113, the incubator 3 located in the outer guide window 111 moves forward and passes through the cleaning mechanism 12. When the incubator 3 is cleaned, the height changes. Under the connection of the universal shaft 114, the connection with the guide linear module 113 will not be affected. The guide linear module 113 can still drag the incubator 3 to continue moving forward, enter the box body 1 after passing through the inner guide window 112, and connect with the forward conveying mechanism.

[0067] Preferably, an electromagnet is installed at the end of the universal shaft 114, and a magnet block connected to the electromagnet by magnetic attraction is installed outside the incubator. When the incubator 3 is located in the viewing window 11, the electromagnet of the universal shaft 114 of the guide linear module 113 is energized, so that the incubator 3 and the universal shaft 114 are always connected, so that the incubator 3 can be driven to move forward along the viewing window 11. After the incubator 3 enters the forward conveying mechanism, the electromagnet of the universal shaft 114 is de-energized, and the universal shaft 114 is disconnected from the incubator 3.

[0068] Furthermore, a humidification assembly 13 is also provided along the outer guide window 111. The humidification assembly 13 includes a top humidification mounting bar 131 installed at the top of the outer guide window 111, a side humidification mounting bar 132 installed at the side of the outer guide window 111, and a bottom humidification mounting bar 133 installed at the bottom of the outer guide window 111. The top humidification mounting bar 131, the side humidification mounting bar 132, and the bottom humidification mounting bar 133 are respectively provided with humidification nozzles 134 along the length direction. After passing through the cleaning mechanism 12, it will pass through the humidification assembly 13. When passing through the humidification assembly 13, the guide linear module 113 stops moving, and the humidification nozzles 134 installed on the top humidification mounting bar 131, the side humidification mounting bar 132, and the bottom humidification mounting bar 133 simultaneously spray water mist to humidify the incubator 3 and provide a better cultivation environment. In addition, the humidification nozzle 134 of the side humidification mounting strip 132 can spray disinfectant, which can kill bacteria on the surface of the incubator 3, further reduce the number of external bacteria entering the box body 1, and further improve the quality of bacterial cultivation.

[0069] Preferably, longitudinally arranged support holes 135 are provided on the sides of the bottom humidification mounting strip 133, longitudinally arranged support columns 136 are installed in the support holes 135, a humidification support plate 137 capable of lifting the incubator 3 is installed on the top of the support column 136, and a humidification lifting cylinder is installed at the bottom of the outer guide window 111. In this embodiment, when passing through the humidification component 13, the guide linear module 113 pauses, and the humidification lifting cylinder can drive the support column 136 to lift the incubator 3, so that there is a gap between the bottom of the incubator 3 and the bottom wall of the outer guide window 111, and the gap is just enough for the humidification nozzle 134 of the bottom humidification mounting strip 133 to spray liquid, thereby ensuring that most of the bottom area of ​​the incubator 3 can be covered by the sprayed liquid.

[0070] After the processes of the cleaning mechanism 12 and the humidifying assembly 13 , the incubator 3 continues to move forward driven by the guide linear module 113 , passes through the inner guide window 112 , enters into the box body 1 , and is connected to the conveying support structure 2 .

[0071] The conveying support structure 2 includes a support conveyor belt 21. Side support bars 211 for sliding and positioning the side of the incubator 3 are respectively arranged on both sides of the support conveyor belt 21 in the forward direction. Support rollers 212 are installed on the top of the side support bars 211 along the length direction. A support conveyor frame 213 is arranged at the bottom of the support conveyor belt 21. The first liquid adding mechanism 22, the second liquid adding mechanism 50 and the liquid extraction mechanism 60 are respectively installed on the support conveyor frame 213. After entering the conveying support structure 2 in the box body 1, under the forward transportation of the support conveyor belt 21, it will pass through the first liquid adding mechanism 22, the second liquid adding mechanism 50 and the liquid extraction mechanism 60 in sequence. Addition or reduction of the culture solution in the incubator 3 is achieved.

[0072] The first liquid adding mechanism 22 includes a first liquid adding support column 220 longitudinally installed on the support conveying frame 213, a longitudinally arranged liquid adding plate 221 is installed on the top of the first liquid adding support column 220, the liquid adding plate 221 is installed with an extrusion module 222 and a liquid injection module 23, the extrusion module 222 includes a liquid injection piston mechanism 223, and the top of the liquid injection piston mechanism 223 is provided with an extrusion liquid inlet 224 and an extrusion liquid outlet 225, the extrusion liquid inlet 224 is connected to a liquid inlet pipe, and the extrusion liquid outlet 225 is connected to a liquid supply pipe 226; the liquid injection module 23 includes a transversely arranged liquid injection plate 231, the liquid injection plate 231 can be longitudinally moved close to or away from the incubator 3, the liquid injection plate 231 is installed with a longitudinally arranged liquid injection head 232, and the top of the liquid injection head 232 is connected to the liquid supply pipe 226. The incubator 3 entering the support conveyor belt 21 is transported forward. After passing through the first liquid adding mechanism 22, the support conveyor belt 21 is suspended, and the extrusion module 222 and the injection module 23 installed on the support conveyor frame 213 are working. Among them, the injection piston mechanism 223 is working. When the piston moves outward (away from the extrusion outlet 225), the volume in the cylinder increases, the pressure decreases, and the liquid is sucked into the cylinder under the action of atmospheric pressure or an external pressure source. When the piston moves inward (toward the extrusion outlet 225), the volume in the cylinder decreases, the pressure increases, and the liquid is squeezed out of the cylinder and injected into the liquid supply pipe 226 through the extrusion outlet 225. In actual application, by accurately controlling the movement stroke and speed of the piston, accurate control of the injection amount and injection speed can be achieved. The liquid in the liquid supply pipe 226 is injected into the incubator 3 through the injection head 232 of the injection module 23.

[0073] In biological laboratories and bacterial culture production, the injection piston mechanism 223 is used to accurately add various chemical reagents. In this solution, in the chemical synthesis reaction of bacterial cultivation, reactants need to be added according to a specific molar ratio, and the injection piston mechanism 223 can accurately control the injection amount of the reagent according to the concentration of the reagent and the amount of the required substance.

[0074] Preferably, the extrusion module 222 also includes a longitudinally arranged extrusion linear module 227, the driving end of which is installed with a longitudinal moving plate 228, the longitudinal moving plate 228 is installed with a longitudinal piston rod 229, the driving end of the longitudinal piston rod 229 is connected to the input end of the injection piston mechanism 223; the output end of the injection piston mechanism 223 is installed with a sealing seat 2291, and the extrusion liquid inlet 224 and the extrusion liquid outlet 225 are respectively formed on the sealing seat 2291. In this embodiment, the movement stroke of the extrusion linear module 227 can determine the movement stroke of the longitudinal moving plate 228, thereby determining the movement stroke of the injection piston mechanism 223, so as to accurately control the injection amount of the liquid. Improve the accuracy of the reaction, improve the quality of bacterial cultivation, and reduce errors.

[0075] In addition, after the liquid injection is completed, the liquid injection head 232 is always arranged downward, and some liquid remaining at the end of the liquid injection head 232 will drip downward into the remaining equipment; to solve the above problems, the liquid injection module 23 also includes a leak-proof plate 235 located below the liquid injection head 232, and also includes a liquid injection lifting cylinder 233 longitudinally installed on the liquid adding plate 221, and the driving end of the liquid injection lifting cylinder 233 is installed with a liquid injection horizontal plate 234, and the liquid injection plate 231 is horizontally installed on the liquid injection horizontal plate 234; in this embodiment, during liquid injection, the liquid injection lifting cylinder 233 works, driving the liquid injection horizontal plate 234 to descend, and the liquid injection head 232 installed on the liquid injection plate 231 descends close to the incubator 3, and the incubator 3 is subjected to the liquid adding operation.

[0076] Specifically, a laterally arranged leak-proof guide rod 236 is connected to the back of the leak-proof plate 235, the liquid adding plate 221 is formed with a leak-proof guide hole 237 for guiding the sliding of the leak-proof guide rod 236, a liquid injection guide plate 238 is installed at the bottom of the liquid injection horizontal plate 234, and the liquid injection guide plate 238 is longitudinally provided with an arc groove 239, and a leak-proof roller 2391 that rolls with the arc groove 239 is installed on the back of the leak-proof plate 235. Since the injection guide plate 238 is provided with an arc groove 239 in the longitudinal direction, the injection guide plate 238 will roll with the leak-proof roller 2391 installed on the back of the leak-proof plate 235 when it moves up and down, and the leak-proof roller 2391 will move along the trajectory of the arc groove 239; when the driving end of the injection lifting cylinder 233 descends, the leak-proof roller 2391 will move to the top along the arc groove 239, at this time, the leak-proof guide rod 236 on the back of the leak-proof plate 235 will slide with the leak-proof guide hole 237 of the liquid adding plate 221, and the leak-proof plate 235 will move away from The injection head 232 will not be hindered from injecting liquid into the incubator 3; when the driving end of the injection lifting cylinder 233 rises and retracts without injecting liquid, the leak-proof roller 2391 will move along the arc groove 239 to the arc at the bottom, and the leak-proof guide rod 236 on the back of the leak-proof plate 235 will slide with the leak-proof guide hole 237 of the liquid adding plate 221, and the leak-proof plate 235 will approach the injection head 232 until the leak-proof plate 235 is located below the injection head 232, so as to collect and store the liquid dripping from the injection head 232 to prevent it from dripping onto other equipment and causing damage.

[0077] A liquid extraction port 321 is provided at the top of the incubator 3, and a sealing module 32 is installed at the liquid extraction port 321. The sealing module 32 includes a sealing column 323 elastically inserted into the liquid extraction port 321. The sealing column 323 is penetrated by a liquid extraction hole 324 with a hollow structure, and the liquid extraction hole 324 can be inserted into the liquid extraction rod 62. The outer ring part of the sealing column 323 is annularly arranged with multiple arc-shaped side ring blocks 325 at equal intervals. An elastic structure for elastically popping out the side ring block 325 is provided between the side ring block 325 and the sealing column 323; a top stopper 328 for blocking the liquid extraction hole 324 is formed on the top of the side ring block 325. In this embodiment, when injecting liquid, the sealing module 32 located at the liquid extraction port 321 will work, and the sealing column 323 will partially pop outward under the action of elasticity. At the same time, the side ring block 325 of the outer ring part of the sealing column 323 will be radially opened under the action of the elastic structure, and the top block 328 on the top of the side ring block 325 cannot block the liquid extraction hole 324. At this time, the liquid injection head 232 can be lowered and inserted into the liquid extraction hole 324 to achieve the liquid addition operation, and the liquid enters the incubator 3 through the sealing module 32 of the liquid extraction port 321. When the liquid injection is completed, the sealing module 32 will retreat to the liquid extraction port 321 under the action of elasticity, and its large elastic force can drive the side ring block 325 to be close to the sealing column 323, and the top block 328 on the top of the side ring block 325 will just block the liquid extraction hole 324. At this time, liquid cannot be injected, ensuring the sealing of the incubator 3 and the quality of the cultivation environment.

[0078] Preferably, a connecting ring 326 is sleeved and formed in the middle area of ​​the sealing column 323, the bottom of the side ring block 325 is rotatably connected to the connecting ring 326 through the shaft hole structure, and a limiting opening 322 is formed at the top of the liquid extraction port 321, and the outer diameter of the limiting opening 322 matches the outer diameter of the side ring block 325. When no liquid is added for extraction, the sealing column 323 of the sealing module 32 is pressed down under the action of elasticity, and at the same time, the side ring block 325 is closely fitted with the sealing column 323 and retracted into the limiting opening 322, and at the same time, the liquid extraction hole 324 is blocked by the top stopper 328 of the side ring block 325. On the contrary, when liquid needs to be added, the sealing column 323 of the sealing module 32 rises partially, and the side ring block 325 is exposed to the outside as a whole. At this time, under the elastic action of the side ring block 325, the bottom of the side ring block 325 swings outward around the connecting ring 326 to be in the expanded state, and the top stopper 328 is away from the liquid extraction hole 324. At this time, liquid can be added or extracted from the incubator 3.

[0079] Specifically, a hole structure 31 is formed on the periphery of the liquid extraction port 321 of the incubator 3, and the hole structure 31 includes a first downward pressure hole 311 and a second downward pressure hole 312. A U-shaped hole 313 is connected between the first downward pressure hole 311 and the second downward pressure hole 312. A U-shaped connecting strip 316 is installed in the U-shaped hole 313. A first movable rod 314 connected to the U-shaped connecting strip 316 is movably installed in the first downward pressure hole 311, and the top of the first movable rod 314 protrudes out of the hole. A first movable rod 314 connected to the U-shaped connecting strip 316 is movably installed in the second downward pressure hole 312. 16, wherein the second movable block 315 is connected to the liquid extraction port 321, and the second movable block 315 is connected to the sealing column 323; initially, the first movable rod 314 rises under the action of elasticity, and the second movable block 315 located at the second downward pressure hole 312 is located near the bottom area of ​​the second downward pressure hole 312, and at the same time, the sealing column 323 of the sealing module 32 is tightly installed in the liquid extraction port 321, sealing and blocking the liquid extraction port 321, and at the same time, the liquid extraction hole 324 of the sealing column 323 is in a blocked state. On the contrary, when the first movable rod 314 is pressed down, the second movable block 315 located at the second downward pressing hole 312 will rise axially under the action of the U-shaped connecting strip 316, thereby driving the sealing column 323 of the sealing module 32 to rise, and the side ring block 325 located at the periphery of the sealing column 323 will radially bounce open under the action of the elastic structure, and the top stop block 328 on the top of the side ring block 325 cannot block the liquid extraction hole 324. At this time, the injection head 232 can be lowered and inserted into the liquid extraction hole 324 to realize the liquid adding operation.

[0080] Specifically, a first compression spring 317 sleeved on the first movable rod 314 is provided in the first pressing hole 311, and the first compression spring 317 can elastically drive the first movable rod 314 to rise, and the elastic structure is a second compression spring 327 connected between the side ring block 325 and the sealing column 323, wherein the elastic force of the first compression spring 317 is greater than the elastic force of the second compression spring 327, the side ring block 325 is arranged longitudinally, the bottom of the side ring block 325 is rotatably connected with the sealing column 323, and the second compression spring 327 can elastically drive the side ring block 325 to elastically pop outward around the bottom end of the sealing column 323. Initially, under the action of the first compression spring 317, the first movable rod 314 located in the first pressing hole 311 will rise a part, thereby driving the U-shaped connecting strip 316 to move along the U-shaped hole 313 close to the first pressing hole 311, and at the same time, the second movable block 315 located in the second pressing hole 312 can move downward to drive the sealing column 323 of the sealing module 32 to be close to the liquid extraction port 321. The liquid extraction port 321 is blocked. Since the elastic force of the first compression spring 317 is greater than the elastic force of the second compression spring 327, the side ring block 325 located on the outside of the sealing column 323 will be squeezed into the limit port 322 and be in a contracted and closed state. The top stopper 328 blocks the liquid extraction hole 324. At this time, the injection head 232 cannot perform liquid adding and liquid extraction operations.

[0081] It should be noted that the first movable rod 314 is formed with radially arranged stop plates along the way, and the first downward pressure hole 311 is formed with an inner movable hole 318 along the way. The inner movable hole 318 allows the stop block 319 to move axially. The first compression spring 317 is located in the inner movable hole 318, and the top of the first compression spring 317 is installed on the bottom of the stop block 319. Under the elastic drive of the first compression spring 317, the first movable rod 314 will bounce upward until the top of the stop block 319 cooperates with the stop of the first downward pressure hole 311, thereby preventing the first movable rod 314 from falling off from the first downward pressure hole 311 as a whole.

[0082] It should be noted that the first liquid adding mechanism 22 and the second liquid adding mechanism 50 have the same working principle and structural arrangement, and produce the same effects, so the specific second liquid adding mechanism 50 will not be described in detail herein.

[0083] After the first liquid adding mechanism 22 and the second liquid adding mechanism 50 respectively add different appropriate amounts of liquid into the incubator 3 , the incubator 3 needs to be shaken to ensure that the liquid inside reacts fully.

[0084] The stirring mechanism 4 includes a stirring robot 40 arranged beside the support and conveying frame 213, the execution end 41 of the stirring robot 40 is equipped with a suction cup module 42, the suction cup module 42 includes a suction cup fixing seat 43, and the suction cup fixing seat 43 is respectively equipped with elastically bendable suction cup frames 44 on both sides, one end of the suction cup frame 44 is movably sleeved on the axis of the suction cup fixing seat 43 through a sleeve, and the suction cup frame 44 is equipped with a plurality of suction cup members 45 that can fit the incubator 3; the execution end 41 of the stirring robot 40 moves to the top of the incubator 3, and under the action of the tension spring The suction cup frame 44 initially has an angle of 90° to 180°. The suction cup frame 44 can be attached to the surface of the incubator 3 according to the curvature of the surface of the incubator 3, and the angle between the two suction cup frames 44 will change. The suction cup frame 45 can be adaptively attached to the incubator 3 through the suction cup frame 45. After the incubator 3 is adsorbed by the suction cup frame 45, the execution end 41 of the stirring robot 40 can be moved to achieve uniform shaking; since the bearing capacity of the suction cup frame 45 is relatively general, the movement speed of the execution end 41 of the stirring robot 40 is relatively low, thereby ensuring the stability of adsorption.

[0085] In addition, the liquid pumping mechanism 60 includes a liquid pumping support plate 61 located beside the second liquid adding mechanism 50 , and a lifting cylinder is installed on the liquid pumping support plate 61 , and a liquid pumping rod 62 is installed on the driving end of the lifting cylinder.

[0086] The liquid extraction mechanism 60 also includes a pressing assembly 46 mounted on the suction cup fixing seat 43, and the pressing assembly 46 includes a pressing block 47, which is arranged upward and the suction cup member 45 is arranged downward. When liquid extraction and liquid addition are required, the execution end 41 of the stirring robot 40 rotates 180° so that the pressing block 47 of the pressing assembly 46 faces downward, and the hole structure 31 of the incubator 3 is pressed by the pressing block 47. When the first movable rod 314 is pressed, the second movable block 315 located at the second pressing hole 312 will rise axially under the action of the U-shaped connecting strip 316, thereby driving the sealing column 323 of the sealing module 32 to rise, and the side ring block 325 located at the periphery of the sealing column 323 will be radially opened under the action of the elastic structure, and the top stopper 328 at the top of the side ring block 325 cannot block the liquid extraction hole 324. At this time, the injection head 232 can be inserted into the liquid extraction hole 324 to achieve the liquid addition operation. After the pressing block 47 moves away from the incubator 3, the first movable rod 314 located at the first pressing hole 311 will rise partially under the action of the first compression spring 317, thereby driving the U-shaped connecting strip 316 to move along the U-shaped hole 313 close to the first pressing hole 311, and at the same time, the second movable block 315 located at the second pressing hole 312 can move downward to drive the sealing column 323 of the sealing module 32 to be close to the liquid extraction port 321. The liquid extraction port 321 is blocked. Since the elastic force of the first compression spring 317 is greater than the elastic force of the second compression spring 327, the side ring block 325 located at the outer side of the sealing column 323 will be squeezed into the limit port 322, in a contracted and close state, and the top stopper 328 blocks the liquid extraction hole 324, and the liquid addition and extraction operations cannot be performed at this time.

[0087] The box body 1 is also formed with a discharge port 115 arranged opposite to the viewing window 11, and the discharge port 115 is hinged with a closed door. After the incubator 3 has been liquid-added, stirred, and pumped for the second time, the stirring robot 40 can adsorb the incubator 3 and adsorb it to the rack for static reaction. The conveying support structure 2 also includes a feed conveyor belt 214 arranged beside the support conveyor belt 21. After the bacteria in the incubator 3 have undergone a series of static reactions and are cultivated and mature, the incubator 3 can be adsorbed by the stirring robot 40 and then transferred to the feed conveyor belt 214. The feed conveyor belt 214 is connected to the discharge port 115, and the incubator 3 can be discharged and taken out through the discharge port 115 for use.

[0088] In summary, it can be seen that the present invention has the above-mentioned excellent characteristics, which can enhance the performance unprecedented in the prior art and become a product with great practical value.

[0089] The above contents are only preferred embodiments of the present invention. For ordinary technicians in this field, according to the concept of the present invention, there will be changes in the specific implementation methods and application scopes. The content of this specification should not be understood as limiting the present invention.

Claims

1. A constant temperature and humidity test box for cultivating bacteria, comprising a box body, a viewing window for entering the incubator, and a temperature and humidity control system, characterized in that: The viewing window is provided with a cleaning mechanism for removing dust from the surface of the incubator, the cleaning mechanism comprising a guide rail structure coaxially arranged outside the viewing window, a cleaning sliding block slidably mounted on the guide rail structure, a cleaning sliding seat connected to a cleaning driving seat, a rotatable brush member mounted on the cleaning driving seat; a driving chain is arranged along the moving track of the cleaning sliding block on the guide rail structure, and a transmission seat movably connected to the cleaning sliding block is mounted on the driving chain; The box body is also provided with a forward conveying mechanism for conveying the incubator, the forward conveying mechanism includes a conveying support structure, and the conveying support structure is sequentially arranged with a first liquid adding mechanism, a second liquid adding mechanism, a stirring mechanism and a liquid pumping mechanism along the way; The conveying support structure comprises a supporting conveyor belt, and side support bars for sliding and positioning the side of the incubator are respectively arranged on both sides of the supporting conveyor belt in the forward direction, and support rollers are installed on the top of the side support bars along the length direction; a supporting conveyor frame is arranged at the bottom of the supporting conveyor belt, and the first liquid adding mechanism, the second liquid adding mechanism and the liquid pumping mechanism are respectively installed on the supporting conveyor frame; The liquid extraction mechanism includes a liquid extraction rod that can move longitudinally; a liquid extraction port is opened on the top of the incubator, and a sealing module is installed on the liquid extraction port. The sealing module includes a sealing column that is elastically inserted into the liquid extraction port, and the sealing column is formed with a liquid extraction hole with a hollow structure, and the liquid extraction hole can be inserted into the liquid extraction rod. The outer ring part of the sealing column is annular and has multiple side ring blocks with arc structures arranged at equal intervals. An elastic structure for elastically popping out the side ring block is arranged between the side ring block and the sealing column; a top stopper for blocking the liquid extraction hole is formed on the top of the side ring block.

2. A constant temperature and humidity test box for cultivating bacteria according to claim 1, characterized in that: An outer guide window is installed on the outer side of the viewing window, and an inner guide window is installed on the inner side of the viewing window, and the inner guide window is connected to the supporting conveyor belt of the cleaning mechanism; the cleaning mechanism is arranged along the outer guide window; a humidifying component is also arranged along the outer guide window, and the humidifying component includes a top humidifying mounting bar installed on the top of the outer guide window, a side humidifying mounting bar installed on the side of the outer guide window, and a bottom humidifying mounting bar installed on the bottom of the outer guide window, and the top humidifying mounting bar, the side humidifying mounting bar and the bottom humidifying mounting bar are respectively arranged with humidifying nozzles along the length direction.

3. A constant temperature and humidity test box for cultivating bacteria according to claim 2, characterized in that: A longitudinally arranged support hole is provided on the side of the bottom humidification mounting strip, and a longitudinally arranged support column is installed in the support hole. A humidification lifting cylinder is installed at the bottom of the outer guide window, and the humidification lifting cylinder can drive the support column to lift the incubator; a guide linear module is arranged along the length direction of the outer guide window, and a universal shaft movably connected to the incubator is installed at the driving end of the guide linear module.

4. A constant temperature and humidity test box for cultivating bacteria according to claim 3, characterized in that: The first liquid adding mechanism includes a first liquid adding support column longitudinally installed on the supporting conveying frame, a longitudinally arranged liquid adding plate is installed on the top of the first liquid adding support column, the liquid adding plate is installed with an extrusion module and an injection module, the extrusion module includes an injection piston mechanism, the top of the injection piston mechanism is provided with an extrusion liquid inlet and an extrusion liquid outlet, the extrusion liquid outlet is connected to a liquid supply pipe; the injection module includes a transversely arranged liquid injection plate, the liquid injection plate can be moved longitudinally to approach or move away from the incubator, the liquid injection plate is installed with a longitudinally arranged liquid injection head, and the top of the liquid injection head is connected to the liquid supply pipe.

5. A constant temperature and humidity test box for cultivating bacteria according to claim 4, characterized in that: The extrusion module also includes a longitudinally arranged extrusion linear module, a driving end of the extrusion linear module is installed with a longitudinal moving plate, the longitudinal moving plate is installed with a longitudinal piston rod, the driving end of the longitudinal piston rod is connected to the input end of the injection piston mechanism; the output end of the injection piston mechanism is installed with a sealing seat, and the extrusion liquid inlet and the extrusion liquid outlet are respectively formed on the sealing seat.

6. A constant temperature and humidity test box for cultivating bacteria according to claim 5, characterized in that: The injection module also includes a leak-proof plate located below the injection head, a leak-proof guide rod arranged laterally is connected to the back of the leak-proof plate, the liquid adding plate is formed with a leak-proof guide hole for guiding the sliding of the leak-proof guide rod, and also includes a liquid injection lifting cylinder longitudinally installed on the liquid adding plate, a liquid injection horizontal plate is installed on the driving end of the liquid injection lifting cylinder, the liquid injection plate is transversely installed on the liquid injection horizontal plate, a liquid injection guide plate is installed at the bottom of the liquid injection horizontal plate, an arc groove is longitudinally opened on the liquid injection guide plate, and a leak-proof roller that rolls with the arc groove is installed on the back of the leak-proof plate.

7. A constant temperature and humidity test box for cultivating bacteria according to claim 6, characterized in that: The stirring mechanism includes a stirring robot arranged beside the supporting conveying frame, the execution end of the stirring robot is equipped with a suction cup module, the suction cup module includes a suction cup fixing seat, and elastically bendable suction cup frames are respectively installed on both sides of the suction cup fixing seat, and the suction cup frames are equipped with multiple suction cup parts that can fit the incubator.

8. A constant temperature and humidity test box for cultivating bacteria according to claim 6, characterized in that: The liquid extraction mechanism also includes a pressing component installed on the suction cup fixing seat, the pressing component includes a pressing block, the pressing block is arranged upward, and the suction cup is arranged downward; a hole structure is formed on the periphery of the liquid extraction port of the incubator, the hole structure includes a first pressing hole and a second pressing hole, a U-shaped hole is connected between the first pressing hole and the second pressing hole, a U-shaped connecting strip is installed in the U-shaped hole, a first movable rod connected to the U-shaped connecting strip is movably installed in the first pressing hole, the top of the first movable rod protrudes out of the hole, and a second movable block connected to the U-shaped connecting strip is movably installed in the second pressing hole, wherein the second pressing hole is connected to the liquid extraction port, and the second movable block is connected to the sealing column.

9. A constant temperature and humidity test box for cultivating bacteria according to claim 8, characterized in that: A first compression spring sleeved on the first movable rod is arranged in the first downward pressure hole, and the first compression spring can elastically drive the first movable rod to rise, and the elastic structure is a second compression spring connected between the side ring block and the sealing column, wherein the elastic force of the first compression spring is greater than the elastic force of the second compression spring, the side ring block is arranged longitudinally, and the bottom of the side ring block is rotatably connected to the sealing column, and the second compression spring can elastically drive the side ring block to elastically pop out outward around the bottom end of the sealing column.

10. A constant temperature and humidity test box for cultivating bacteria according to any one of claims 1 to 9, characterized in that: The temperature and humidity control system includes a humidifier and an air conditioner, both of which are arranged outside the box. A pipe is provided at the outlet of the humidifier to connect to the box; a pipe is provided at the outlet of the air conditioner to connect to the box, and a temperature sensor and a humidity sensor are provided in the box.

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