Multi-index blood coagulation item detection system

Abstract

The multi-index blood coagulation item detection system comprises a shell, a detection heating module, a battery module, a touch display screen and a mainboard, the shell comprises an upper shell body and a lower shell body, the upper shell body and the lower shell body are in butt joint to define a closed space, and the mainboard is arranged between the upper shell body and the lower shell body and arranged in the closed space; the touch display screen is arranged on the upper surface of the upper shell, and the touch display screen is connected with the mainboard; the detection heating module is arranged in the closed space, is arranged at the front end of the lower shell and is connected with the mainboard, a battery module is arranged at the bottom of the tail end of the lower shell and is connected with the mainboard, and a detection inlet for inserting a test card is formed in the front end of the shell; the positions, corresponding to the battery modules, of the two sides of the main board are each provided with at least one battery elastic piece. And the test card adopts a multi-channel micro-flow detection chip. The multi-index blood coagulation item detection system can perform real-time detection, can be operated without professionals, and is high in sensitivity, convenient to operate and high in detection accuracy.

Description

[0001] This application is a divisional application of the Chinese invention patent application with application number "201910154647.2", application date "March 01, 2019", and invention name "Multi-index coagulation project detection system". Technical Field

[0002] The present invention relates to the technical field of rapid detection in vitro diagnostic equipment, and in particular to a multi-index coagulation item detection system. Background Art

[0003] Traditional coagulation testing requires the patient's plasma to be drawn intravenously into a sodium citrate anticoagulant tube, and then the upper plasma is transferred to a large coagulation analyzer for testing after centrifugation as required. The test results take a long time, and the patient needs to go to the hospital to complete it. Especially for those patients with thrombosis who need to take anticoagulants for a long time, frequent visits to the hospital for reexamination are time-consuming and labor-intensive. Currently, the coagulation analyzers on the market are mainly based on two methods: magnetic bead method and optical method. The magnetic bead method uses the principle of generating electrical signals by the swing of magnetic beads cutting magnetic lines of force. The coagulation point is determined by monitoring the swing amplitude of the magnetic beads. When the swing floating attenuation of the magnetic beads is 50%, the coagulation point is considered to have been reached. This method is costly and has high requirements for equipment manufacturing technology; the optical method determines the coagulation point by optically analyzing the turbidity changes during the blood coagulation process. The optical method has low cost and high precision. Currently, new coagulation analyzers generally use the optical method. The above two detection methods are not intuitive enough, and the detection equipment is relatively complex and expensive, the detection cost is also high, and the monitoring accuracy is often unsatisfactory due to equipment or environmental factors.

[0004] A Chinese patent document (application number: 201810190682.5) discloses a portable coagulation test card, which includes: a bottom layer printed with detection electrodes, a middle layer through which blood samples flow, and an upper layer for siphoning blood samples. The upper layer, the middle layer, and the bottom layer are sequentially pasted together; the upper layer is provided with a sample addition area for adding blood samples; the middle layer is provided with at least two test channels, one end of the test channel is connected to the sample addition area, and the other end is provided with a test area; one of the test channels is a delay channel, and an obstacle is provided in the middle of the delay channel. An air hole connected to the upper layer is provided at the end of the test area; an electrode conduction area connected to the test area is provided on the inner side wall of the bottom layer; and the end of the electrode conduction area is inserted into the test host for detection. This portable coagulation test card cannot detect five coagulation indicators at the same time, the detection time is long, and the sample needs to be pre-treated.

[0005] Chinese patent document (application number: 201721885245.2) discloses a handheld blood analyzer, including a blood analyzer, a power switch, a display screen and a handle fixing seat, the display screen is arranged on the panel of the blood analyzer, the power switch is arranged below the display screen, an LED light is arranged on one side of the power switch, a detection window is arranged on one side of the LED light, a detection area door is installed at the detection window, a handle is installed on the detection area door, a sample placement slot is arranged at the bottom of the detection window, a detection head and an infrared sensor are arranged above the detection window, a handle is installed just above the blood analyzer, the handle is fixed to the blood analyzer through the handle fixing seat, a main control circuit board is arranged inside the blood analyzer, a photoelectric sensor, a filter, a signal receiver, a processor and a signal transmitter are arranged below the main control circuit board, a built-in WIFI is arranged on one side of the signal transmitter, a battery is arranged below the photoelectric sensor, and a charging line interface is arranged on one side of the battery. The handheld blood analyzer device is relatively large in size, complex in structure, and high in detection cost.

[0006] Therefore, it is necessary to provide a multi-index coagulation project detection system that can be tested instantly, does not require professional personnel to operate, has high sensitivity, is easy to operate, and has a constant heating temperature. Summary of the invention

[0007] The present invention provides a multi-index blood coagulation item detection system which can detect instantly, can be operated without professional personnel, has high sensitivity, is easy to operate, and has a constant heating temperature.

[0008] In order to solve the above technical problems, the technical solution of the present invention is implemented as follows: the multi-indicator coagulation project detection system includes a shell, a detection and heating module, a battery module, a touch display screen and a main board, the shell includes an upper shell and a lower shell, and the shell is connected by the upper shell and the lower shell to form a closed space, the main board is arranged between the upper shell and the lower shell and arranged in the closed space; the touch display screen is arranged on the upper surface of the upper shell, and the touch display screen is connected to the main board; the detection and heating module is arranged in the closed space and at the front end of the lower shell and connected to the main board, a battery module is provided at the bottom of the end of the lower shell and the battery module is connected to the main board, and a detection entrance for inserting a test card is provided at the front end of the shell.

[0009] By adopting the above technical scheme, the test card is inserted into the detection heating module from the detection entrance and forms a conductive connection with the detection heating module, so as to sense the changes in the electrical signals of the coagulation process. The touch screen prompts to add the sample, and the blood from the fingertips drips into the sample adding hole of the test card. The whole blood sample is then quickly and evenly distributed into each reaction chamber of the test card. The multi-index coagulation project detection system detects the signal of the sample entering the reaction chamber, and uses the AC impedance method to monitor the signal, applies AC voltage, and heats the heating plate to keep the temperature controlled at 37 degrees. As the sample coagulation process, the electrical signal changes, and the detection heating module of the multi-index coagulation project detection system collects the signal changes and performs operational amplifier; after the reaction, the multi-index coagulation project detection system fits a complete reaction curve based on all the received signals, and uses a mathematical algorithm to calculate the signal point, which is the detection result. The multi-index coagulation project detection system can detect instantly and can be operated without professional personnel. It has high sensitivity, convenient operation, reliable function and wide application range. The touch display screen is fixed on the upper shell by screws, the mainboard is embedded between the upper shell and the lower shell and fixed on the lower shell by screws, and the detection and heating module is fixed to the bottom of the lower shell by screws; the outer shell is sealed and connected by screws to form a closed space.

[0010] As a preferred technical solution of the present invention, the front end of the lower shell is also provided with a code scanning module, which is connected to the main board. The test card is placed in the multi-index coagulation project detection system and scanned by the code scanning module before detection to identify the sample information and store it.

[0011] The present invention is further improved in that the detection and heating module includes a card slot, a heating plate, a heating plate card slot, a temperature sensor 1, a PCB board and a test card connector, wherein the test card connector is arranged on the PCB board, and the test card connector is connected to the main board through a circuit connector; the heating plate is installed in the heating plate card slot for constant temperature heating, and the heating plate card slot is installed on the PCB board on one side of the test card connector; the temperature sensor 1 is arranged below the heating plate and in the heating plate card slot; the card slot is installed on the upper surface of the heating plate and is engaged with the heating plate card slot and the PCB board in turn. Such a structure makes it more convenient to connect the test card with the detection and heating module, and also makes the detection result of the detection and heating module more accurate; the temperature sensor 1 is used to monitor the temperature of the heating plate, and then the heating of the heating plate is controlled by the CPU of the main board, so as to achieve the heating plate to maintain a constant temperature.

[0012] A further improvement of the present invention is that the test card connector contains a spring sheet, and the test card connector is pressed into contact with the conductive electrodes of the test card through the spring sheet to form a conductive connection.

[0013] The present invention is further improved in that both sides of the heating plate slot are provided with insert slots, both sides of the slot are provided with inserts, the heating plate slot is provided with a heating plate connection hole extending horizontally on both sides of one end near the test card connector, one end of the heating plate is provided with two connectors, and the connector is connected to the main board through the heating plate connection hole; the middle of the heating plate slot near one end of the test card connector is recessed downward and provided with a temperature sensor slot; the position on the PCB board connected with the heating plate slot is recessed inward and penetrates the thickness of the PCB board and is provided with a square hole, and the front ends of both sides of the square hole are extended outward to provide an open square slot; the PCB board is recessed inward between the square hole and the test card connector and is provided with two square slots; the heating plate slot and the slot are respectively engaged with the open square slot and the square slot through the insert slot. Such a structure is conducive to better connection of the components in the detection heating module and is also more convenient for maintenance. The temperature sensor is placed in the temperature sensor slot at the bottom of the heating plate, which can better monitor the temperature of the heating plate.

[0014] As a preferred technical solution of the present invention, the temperature sensor 1 is embedded in the temperature sensor slot and is connected to the main board through a flexible joint connected to the temperature sensor 1 and passes through the square hole; the connecting head is a flexible connecting head, and the flexible connecting head passes through the heating plate connecting hole downward through the open square slot and is connected to the main board.

[0015] As a preferred technical solution of the present invention, the detection entrance is located at one side of the front end of the upper shell, the detection entrance passes through the upper shell to the main board and extends to the lower shell, the detection entrance is concave inward and arc-shaped from the upper shell and extends to the arc-shaped detection entrance of the main board and the lower shell in sequence, and is connected with the platform formed by the lower shell extending parallel to the inside of the lower shell from the horizontal direction to form an inner arch detection entrance, so that the sample loading hole after the test card and the detection heating module are plugged and connected is located at the inner arch detection entrance, and the test card is located above the heating plate, and the electrode end of the test card is inserted into the test card connector. The inner arch detection entrance exposes the sample loading hole of the test card to the outside and makes the sample loading hole flat on the platform of the lower shell, so that the test blood sample can enter the detection chamber from the sample loading hole of the test card better and faster; at the same time, the inner arch detection entrance is more in line with the shape of the fingertip, which is convenient for inserting and placing the test card and collecting the blood sample of the finger.

[0016] The present invention is further improved in that the front end of the mainboard is provided with a CPU, a barcode scanner cable holder, an electrode acquisition amplifier module and a display cable holder, and the end of the mainboard is provided with a battery management module and a power interface; the middle part of the mainboard is provided with a WiFi module, a Bluetooth module and a temperature sensor 2; the barcode scanner module is bidirectionally connected to the CPU through the barcode scanner cable holder; the test card is bidirectionally connected to the CPU through the electrode acquisition amplifier module; the touch display screen is bidirectionally connected to the CPU through the display cable holder; the battery management module can control the charge and discharge of the battery module through the power interface, and the power interface is embedded in the bottom of the shell; the battery management module, WiFi module and Bluetooth module are bidirectionally connected to the CPU; the detection and heating module and the temperature sensor 2 are unidirectionally connected to the CPU. The modules on the mainboard cooperate with each other to realize instant detection, and the detection sensitivity is accurate, and the detection result is obtained within 8 minutes; the temperature sensor 2 is used to monitor the ambient temperature used by the multi-index coagulation project detection system.

[0017] As a preferred technical solution of the present invention, a switch button is provided on the side of the touch display screen at one end of the upper shell close to the detection entrance, and the main board also includes a key board wiring socket and a switch circuit, and the switch button is connected to the main board through the key board wiring socket; the main board controls the switch button through the switch circuit.

[0018] As a preferred technical solution of the present invention, a status light is provided above one side of the touch display screen in the upper shell, and the status light is connected to the main board through a status light cable holder. The status of the status light is observed through a small hole provided at a corresponding position on the surface of the upper shell; the status light displays the power status and whether the test result is normal or not through different colors.

[0019] As a preferred technical solution of the present invention, at least one battery shrapnel is provided on both sides of the mainboard at a position corresponding to the battery module, and the battery is connected to the mainboard through the battery shrapnel to supply power to the mainboard. Two battery shrapnel are provided on both sides of the mainboard for installing batteries to supply power to the mainboard.

[0020] As a preferred technical solution of the present invention, a communication interface is also embedded at the end of the shell, and the communication interface passes through the shell and is fixed on the mainboard.

[0021] As a preferred technical solution of the present invention, the spring sheet has a supporting portion, which extends horizontally and then bends downward and inward to form a clamping portion, and the clamping portion extends upward and inward to form the end portion of the spring sheet, and the spring sheet contacts the conductive electrode of the test card through the clamping portion.

[0022] As a preferred technical solution of the present invention, the lower shell is provided with an anti-slip strip at the position of the detection heating module; the upper surface of the card slot is provided with a plurality of inner slots recessed along the thickness direction thereof; a total of 4 inserts are provided on both sides of the card slot, and accordingly, the number of insert slots of the heating plate card slot is 4. The PCB board is recessed inwardly between the square hole and the test card connector and is penetrated with two square slots.

[0023] As a preferred technical solution of the present invention, the temperature sensor 1 in the detection and heating module is unidirectionally connected to the CPU. The temperature sensor 1 in the detection and heating module is unidirectionally connected to the CPU of the mainboard through a flexible joint passing through a square hole; the temperature sensor 1 is used to monitor the temperature of the heating plate.

[0024] As a preferred technical solution of the present invention, the upper surface of the card slot is provided with 5 inner slots; the test card has 5 coagulation index tests, and accordingly, the test card is bidirectionally connected to the CPU through the electrode acquisition amplifier module in a 5-way channel. The role of setting the inner slots is to strengthen the module structure, and the number of inner slots is preferably 5. Other numbers of inner slots can be designed according to the actual volume size, etc., as long as they can play the role of strengthening the module structure.

[0025] As a preferred technical solution of the present invention, the mainboard also includes a base connector and a printer module, the base connector is used to connect a printer or charge, and the printer module is bidirectionally connected to the CPU. The printer module is optional and can be set as needed. With the addition of the printer module, the multi-index coagulation project detection system can be directly connected to the printer to directly print out the results. The base connector can be used for charging and can also be used to connect a printer.

[0026] As a preferred technical solution of the present invention, the test card adopts a multi-channel microfluidic detection chip, which includes a sample loading hole, a plurality of independent detection chambers, a microfluidic channel and a conductive electrode. The sample loading hole is connected to the detection chamber through a microfluidic channel, and the detection chamber is connected to the conductive electrode. The microfluidic channel includes a mainstream channel and a plurality of branch microfluidic channels. The end of the mainstream channel branches out into a plurality of branch fluid channels, and the plurality of branch fluid channels are connected to a plurality of independent detection chambers in a one-to-one correspondence. The front end of the mainstream channel is connected to the sample loading hole. The multi-channel microfluidic detection chip is designed with a main channel and multiple sub-microfluidic channels in a specific structural form to guide the flow of blood samples, so that one sample chamber can inject samples into multiple reaction chambers at the same time without contaminating the samples, making it easy to inject samples; after sampling at the sampling hole, the main channel flows to multiple sub-microfluidic channels at the same time, and then enters multiple independent detection chambers, which are pre-embedded with detection reagents. This can achieve simultaneous detection of multiple samples and has a multi-channel effect; the chip has a simple structure and is easy to operate, which improves detection efficiency and greatly reduces resource consumption; it achieves rapid detection and reduces costs.

[0027] Compared with the prior art, the invention has the following beneficial effects: the multi-index coagulation item detection system has a simple structure and controllable operation, the heating plate contains a temperature sensor 1, a nested structure, the detection module has a simple and compact structure, the constant temperature control is precise, the temperature of the entire detection process is controlled at 37±0.1°C, and the cost is saved; the multi-index coagulation item detection system realizes instant detection, the detection is efficient, sensitive and accurate, and the test result can be obtained within 8 minutes; by connecting a multi-channel microfluidic detection chip (test card), 5 coagulation indicators can be detected simultaneously (five coagulation indicators: PT (INR), APTT, TT, FIB, ACT), and other indicators can also be detected according to different detection items, and blood can be directly drawn through the fingertips without processing, which is easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a structural diagram of the multi-index coagulation item detection system of the present invention;

[0029] Figure 2 It is an exploded structural diagram of the upper shell of the multi-index blood coagulation project detection system of the present invention;

[0030] Figure 3 It is an internal diagram of the lower shell of the multi-index blood coagulation project detection system of the present invention;

[0031] Figure 4 It is a structural diagram of the mainboard of the multi-index coagulation project detection system of the present invention;

[0032] Figure 5 It is a structural exploded diagram of the detection and heating module of the multi-index coagulation item detection system of the present invention;

[0033] Figure 6 It is a vertical cross-sectional three-dimensional structural diagram of the detection and heating module of the multi-index blood coagulation item detection system of the present invention from the position of the temperature sensor 1;

[0034] Figure 7 It is a structural diagram of a test card of a multi-index blood coagulation item detection system of the present invention;

[0035] Figure 8 It is a partial enlarged view of the connection between the test card connector and the test card of the multi-index coagulation item detection system of the present invention;

[0036] Fig. 9 It is a circuit schematic diagram of the multi-index blood coagulation project detection system of the present invention;

[0037] Fig.10 It is a schematic diagram of a coagulation index (APTT) detection curve of the multi-index coagulation item detection system of the present invention;

[0038] Among them: 1-shell; 101-upper shell; 102-lower shell; 103-anti-slip strip; 2-code scanning module; 3-test card; 301-sample adding hole; 4-detection heating module; 401-card slot; 4011-insert; 4012-inner slot; 402-heating plate; 4021-connector; 403-heating plate card slot; 4031-insert slot; 4032-heating plate connecting hole; 4033-temperature sensor slot; 404-temperature sensor 1; 4041-flexible joint; 405-PCB board; 4051-square hole; 4052-open square slot; 4053-square slot; 406 -test card connector; 4061-shrapnel; 5-battery module; 501-battery management module; 502-power interface; 6-touch screen; 7-motherboard; 701-CPU; 702-barcode scanner cable holder; 703-electrode acquisition amplifier module; 704-display cable holder; 705-base connector; 706-WiFi module; 707-Bluetooth module; 708-keyboard cable holder; 709-status light cable holder; 7010-temperature sensor 2; 7011-communication interface; 7012-battery shrapnel; 8-detection entrance; 9-switch button; 10-status light. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiment diagram of the present invention.

[0040] Example: Figures 1 to 9As shown, the multi-index coagulation item detection system includes a shell 1, a detection and heating module 4, a battery module 5, a touch display screen 6 and a main board 7, the shell 1 includes an upper shell 101 and a lower shell 102, and the shell 1 is enclosed by the upper shell 101 and the lower shell 102 to form a closed space, the main board 7 is arranged between the upper shell 101 and the lower shell 102 and arranged in the closed space; the touch display screen 6 is arranged on the upper surface of the upper shell 101, and the touch display screen 6 is connected to the main board 7; the detection and heating module 4 is arranged in the closed space and at the front end of the lower shell 102 and connected to the main board 7, the battery module 5 is provided at the bottom of the end of the lower shell 102 and the battery module 5 is connected to the main board 7, and the front end of the shell 1 is provided with a detection entrance 8 for inserting and placing the test card 3. A code scanning module 2 is also provided at the front end of the lower shell 102 on the side of the detection entrance 8, and the code scanning module 2 passes through the lower shell 102 from the outer surface of the lower shell 102 and is connected to the main board 7 in the outer shell 1; wherein the touch display screen 6 is fixedly embedded in the upper shell 101 by screws, the main board 7 is embedded between the upper shell 101 and the lower shell 102 and fixed to the lower shell 102 by screws, and the detection and heating module 4 is fixed to the bottom of the lower shell 102 by screws; the outer shell 1 is sealed and connected by screws to form a closed space; the detection and heating module 4 includes a card slot 401, a heating plate 402, a heating plate card slot 403, a temperature sensor 404, a PCB board 405 and a test card connector 406, and the test card connector 406 The heating plate 402 is arranged on the PCB board 405, and the heating plate 402 is installed in the heating plate slot 403 for constant temperature heating. The heating plate slot 403 is installed on the PCB board 405 and is located on one side of the test card connector 406; the temperature sensor 404 is arranged below the heating plate 402 and in the heating plate slot 403; the slot 401 is installed on the upper surface of the heating plate 402 and is sequentially engaged with the heating plate slot 403 and the PCB board 405; the test card connector 406 is connected to the main board 7 through a circuit connector; the test card connector 406 contains a spring 4061, and the test card connector 406 is pressed and contacted with the conductive electrode of the test card 3 through the spring 4061 to form a conductive connection;Insertion slots 4031 are provided on both sides of the heating plate slot 403, and inserts 4011 are provided on both sides of the slot 401. Heater connecting holes 4032 are provided on both sides of the heating plate slot 403 near the end of the test card connector 406, extending in the horizontal direction. Two connectors 4021 are provided at one end of the heating plate 402, and the connectors 4021 are connected to the main board 7 through the heating plate connecting holes 4032. A temperature sensor slot 4033 is provided in the middle of the heating plate slot 403 near the end of the test card connector 406; the position on the PCB board 405 connected to the heating plate slot 403 is recessed inward and is provided with a square through the thickness of the PCB board. The front ends of both sides of the square hole 4051 are extended outward to form open square grooves 4052. The temperature sensor 404 is embedded in the temperature sensor groove 4033 and is connected to the main board 7 through the flexible joint 4041 connected to the temperature sensor 404 through the square hole 4051; the connector is a flexible connector, which passes downward through the open square groove 4052 through the heating plate connecting hole 4032 and is connected to the main board 7; the PCB board 405 is recessed inwardly between the square hole 4051 and the test card connector 406 and is penetrated by two square grooves 4053; the heating plate card slot 403 and the card slot 401 are connected to each other through the insert 4011 through the insert slot 4031 is respectively engaged with the open square groove 4052 and the square groove 4053; the detection entrance 8 is located on one side of the front end of the upper shell 101, and the detection entrance 8 passes through the main board 7 from the upper shell 101 and extends to the lower shell 102. The detection entrance 8 is concave inwardly and arc-shaped downwardly from the upper shell 101 and extends to the arc-shaped detection entrance of the main board and the lower shell in sequence, and is connected with the platform formed by the lower shell 102 extending from the horizontal direction to the inside of the lower shell 102 in parallel to form an inner arch detection entrance, so that the test card 3 is plugged into the detection heating module 4, the test card 3 is above the heating plate 402, and the electrode end of the test card 3 is inserted into the test card connector 406. After the connection, the sample loading The hole 301 is located at the inner arch detection entrance; the front end of the main board 7 is provided with a CPU 701, a barcode scanner cable holder 702, an electrode acquisition amplifier module 703 and a display cable holder 704, and the end of the main board 7 is provided with a battery management module 501 and a power interface 502; the middle part of the main board 7 is provided with a WiFi module 706, a Bluetooth module 707 and a temperature sensor 2 7010; the barcode scanner module 2 is bidirectionally connected to the CPU 701 through the barcode scanner cable holder 702; the test card 3 is bidirectionally connected to the CPU 701 through the electrode acquisition amplifier module 703; the touch display screen 6 is bidirectionally connected to the CPU 701 through the display cable holder 704;The battery management module 501 can control the charge and discharge of the battery module 5 through the power interface 502, and the power interface 502 is embedded in the bottom of the shell 1; the battery management module 501, the WiFi module 706 and the Bluetooth module 707 are all bidirectionally connected to the CPU 701; the detection and heating module 4 and the temperature sensor 1 404 in the detection and heating module 4 and the temperature sensor 2 7010 on the main board 7 are all unidirectionally connected to the CPU 701; the upper shell 101 is provided with a switch button 9 on the side of the touch display screen 6 at one end close to the detection entrance 8, and the main board 7 also includes a key board cable The switch button 9 is connected to the main board 7 through the keypad cable holder 708, and the main board 7 controls the switch button 9 through the switch circuit; a status light 10 is provided above one side of the touch display screen 6 in the upper shell 101, and the status light 10 is connected to the main board through the status light cable holder 709, and the status light 10 is observed through a small hole provided at a corresponding position on the surface of the upper shell 101; the status light 10 displays the power status and whether the test result is normal or not through different colors; two battery modules are provided on both sides of the main board 7 at positions corresponding to the battery module 5 The spring clip 7012, i.e., a total of 4 battery spring clips 7012, the battery is connected to the main board 7 through the battery spring clip 7012 to supply power to the main board 7; the end of the shell 1 is also inlaid with a communication interface 7011, the communication interface 7011 passes through the shell 1 and is fixed on the main board 7; the spring clip 4061 has a supporting portion, the supporting portion extends horizontally and then bends downward and inward to form a pressing portion, the pressing portion extends upward and inward to form the end portion of the spring clip 4061, and the spring clip 4061 contacts the conductive electrode of the test card 3 through the pressing portion; the lower shell 102 is provided with a protective cover at the position of the detection heating module 4 Slide bar 103; the upper surface of the card slot 401 is provided with a plurality of inner slots 4012 recessed along the thickness direction thereof; a total of 4 inserting pieces 4011 are provided on both sides of the card slot 401, and correspondingly, the number of inserting piece slots 4031 of the heating plate card slot 403 is 4; the PCB board 405 is recessed inwardly between the square hole 4051 and the test card connector 406 and is penetrated by two square slots 4053; the upper surface of the card slot 401 is provided with 5 inner slots 4012; the test card 3 has 5 coagulation index tests, and correspondingly, the test card 3 is bidirectionally connected to the CPU 701 through the electrode acquisition amplifier module 703 in a 5-way channel;The test card 3 is a multi-channel microfluidic detection chip, which includes a sample loading hole 301, 5 independent detection chambers, a microfluidic channel and a conductive electrode. The sample loading hole is connected to the detection chamber through the microfluidic channel, and the detection chamber is connected to the conductive electrode. The microfluidic channel includes a main channel and 5 branch microfluidic channels. The end of the main channel branches out 5 branch fluid channels, and the 5 branch fluid channels are connected to 5 independent detection chambers one by one. The front end of the main channel is connected to the sample loading hole. ;

[0041] Optionally, the mainboard 7 of the multi-index coagulation project detection system further includes a base connector 705 and a printer module, wherein the base connector 705 is used to connect a printer, and the printer module is bidirectionally connected to the CPU 701. The printer module is optional and can be set as needed. By adding the printer module, the multi-index coagulation project detection system can be directly connected to the printer to directly print out the results. The base connector can be used for charging and can also be used to connect a printer.

[0042] During use: put the test card 3 into the multi-index coagulation project detection system. Before the detection, it is necessary to scan the code through the code scanning module 2 to identify the sample information and store the information. Then insert the test card 3 into the detection entrance 8. The test card connector 406 of the detection heating module 4 forms a conductive connection with the detection heating module 4 through the spring 4061, senses the change of the electrical signal in the coagulation process, and the touch display screen 6 prompts to add the sample. The fingertip blood drips into the sample addition hole of the test card 3, and the whole blood sample is quickly and evenly distributed into the five reaction chambers of the test card 3. The multi-index coagulation project detection system detects the signal of the sample entering the reaction chamber, uses the AC impedance method for signal monitoring, applies AC voltage, and heats the heating plate 402. The temperature sensor 1 is used for monitoring to keep the temperature of the heating plate at 37 degrees, even if the heating plate achieves constant temperature (the temperature test data of the heating plate is shown in Table 1 below). As the sample coagulation process, the electrical signal changes, and the test card connector of the multi-index coagulation project detection system collects the signal, passes through the electrode acquisition amplifier module, and performs calculation control in the CPU. After the reaction is completed, the multi-index coagulation test system fits the complete reaction curve based on all the received signals, and uses mathematical algorithms to calculate the signal point, which is the test result. The coagulation index PT is substituted into the equation to calculate the INR value based on the test result. The PT (INR), APTT, TT, FIB, and ACT test results appear on the display screen interface at the end of the measurement, and the entire measurement process is completed. The coagulation index (e.g. APTT) test curve is as follows Fig.10 As shown in the figure, the time corresponding to the lowest point of the curve is the coagulation time.

[0043] Table 1 Temperature monitoring of heating module (37℃ constant temperature effect) data table

[0044] Time (seconds) Temperature(℃) 10 37.02 20 37.03 30 37.00 40 36.98 50 36.98 60 37.00 70 37.01 80 36.99 90 36.97 100 37.00 110 37.01 120 37.00 130 36.99 140 37.01 150 37.00 160 37.04 170 37.00 180 36.99 190 37.00 200 36.98

[0045] According to monitoring, the heating module can maintain a constant temperature of 37°C during the detection process, which makes the test results more accurate.

[0046] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, such as making some other slight adjustments to the shape and structure of the detection entrance. These changes and improvements fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A multi-index coagulation project detection system, characterized in that: The multi-index coagulation item detection system comprises a shell, a detection and heating module, a battery module, a touch display screen and a main board, wherein the shell comprises an upper shell and a lower shell, and the shell is enclosed by the upper shell and the lower shell being connected to form a closed space, the main board is arranged between the upper shell and the lower shell and arranged in the closed space; the touch display screen is arranged on the upper surface of the upper shell, and the touch display screen is connected to the main board; the detection and heating module is arranged in the closed space and at the front end of the lower shell and connected to the main board, the battery module is arranged at the bottom of the end of the lower shell and the battery module is connected to the main board, and the front end of the shell is provided with a detection entrance for inserting and placing a test card; At least one battery spring is disposed on both sides of the mainboard at positions corresponding to the battery module, and the battery is connected to the mainboard through the battery spring to supply power to the mainboard; The test card adopts a multi-channel microfluidic detection chip, which includes a sample loading hole, a plurality of independent detection chambers, a microfluidic channel and a conductive electrode. The sample loading hole is connected to the detection chamber through the microfluidic channel, and the detection chamber is connected to the conductive electrode. The microfluidic channel includes a mainstream channel and a plurality of branch microfluidic channels. The end of the mainstream channel branches out into a plurality of branch fluid channels, and the plurality of branch fluid channels are connected to a plurality of independent detection chambers in a one-to-one correspondence. The front end of the mainstream channel is connected to the sample loading hole.

2. The multi-index coagulation item detection system according to claim 1, characterized in that: A code scanning module is also provided at the front end of the lower shell on one side of the detection entrance, and the code scanning module is connected to the main board.

3. The multi-index coagulation item detection system according to claim 2, characterized in that: The detection and heating module includes a card slot, a heating plate, a heating plate card slot, a temperature sensor 1, a PCB board and a test card connector, wherein the test card connector is arranged on the PCB board and connected to the main board through a circuit connector; the heating plate is installed in the heating plate card slot for constant temperature heating, and the heating plate card slot is installed on the PCB board at one side of the test card connector; the temperature sensor 1 is arranged below the heating plate and in the heating plate card slot; the card slot is installed on the upper surface of the heating plate and is sequentially engaged with the heating plate card slot and the PCB board.

4. The multi-index coagulation item detection system according to claim 3, characterized in that: The test card connector contains a spring sheet, and the test card connector is pressed into contact with the conductive electrode of the test card through the spring sheet to form a conductive connection.

5. The multi-index coagulation item detection system according to claim 3, characterized in that: Insertion slots are provided on both sides of the heating plate slot, and inserts are provided on both sides of the slot. Heater connecting holes are extended and penetrated in the horizontal direction on both sides of the heating plate slot near one end of the test card connector, and two connectors are provided at one end of the heating plate, and the connectors are connected to the main board through the heating plate connecting holes; a temperature sensor slot is recessed downward in the middle of one end of the heating plate slot near the test card connector; a square hole is recessed inwardly and penetrates the thickness of the PCB board at a position connected to the heating plate slot on the PCB board, and open square slots are extended outward at the front ends of both sides of the square hole; the PCB board is recessed inwardly between the square hole and the test card connector and penetrated with two square slots; the heating plate slot and the slot are respectively engaged with the open square slot and the square slot through the inserting piece passing through the inserting slot.

6. The multi-index coagulation item detection system according to claim 5, characterized in that: The temperature sensor 1 is embedded in the temperature sensor slot and is connected to the main board through the square hole via a flexible joint connected to the temperature sensor 1; the connector is a flexible connector, which passes through the heating plate connection hole downward through the open square slot and is connected to the main board.

7. The multi-index coagulation item detection system according to claim 4, characterized in that: The detection entrance is located on one side of the front end of the upper shell, and the detection entrance passes through the upper shell to the main board and extends to the lower shell. The detection entrance is concave inward and arc-shaped downward from the upper shell and extends to the arc-shaped detection entrance of the main board and the lower shell in sequence, and is connected to the platform formed by the lower shell extending parallel to the inside of the lower shell from the horizontal direction to form an inner arch detection entrance, so that the test card is plugged into the detection heating module, and the sample loading hole after connection is located at the inner arch detection entrance, and the test card is located above the heating plate, and the electrode end of the test card is inserted into the test card connector.

8. The multi-index coagulation item detection system according to claim 3, characterized in that: The front end of the mainboard is provided with a CPU, a barcode scanner cable socket, an electrode acquisition amplifier module and a display screen cable socket, and the upper end of the mainboard is provided with a battery management module and a power interface; the middle part of the mainboard is provided with a WiFi module, a Bluetooth module and a second temperature sensor; the barcode scanner module is bidirectionally connected to the CPU through the barcode scanner cable socket; the test card is bidirectionally connected to the CPU through the electrode acquisition amplifier module in a multi-channel manner; the touch display screen is bidirectionally connected to the CPU through the display screen cable socket; the battery management module can control the charging and discharging of the battery module through the power interface, and the power interface is embedded in the bottom of the shell; the battery management module, the WiFi module and the Bluetooth module are all bidirectionally connected to the CPU; the detection and heating module and the second temperature sensor are both unidirectionally connected to the CPU.

9. The multi-index coagulation item detection system according to claim 8, characterized in that: The upper shell is provided with a switch button on the side of the touch display screen at one end close to the detection entrance. The main board also includes a key board cable holder and a switch circuit. The switch button is connected to the main board through the key board cable holder; the main board controls the switch button through the switch circuit.

10. The multi-index coagulation item detection system according to claim 9, characterized in that: A status light is provided above one side of the touch display screen in the upper shell, and the status light is connected to the mainboard via a status light cable holder. The status of the status light is observed through a small hole provided at a corresponding position on the surface of the upper shell; the status light displays the power status and whether the detection result is normal or not through different colors.

11. The multi-index coagulation item detection system according to claim 10, characterized in that: A communication interface is also embedded at the end of the shell, and the communication interface passes through the shell and is fixed on the mainboard.

12. The multi-index coagulation item detection system according to claim 7, characterized in that: The spring sheet has a supporting portion, which extends horizontally and then bends downward and inward to form a pressing portion, and the pressing portion extends upward and inward to form the end portion of the spring sheet, and the spring sheet contacts the conductive electrode of the test card through the pressing portion.

13. The multi-index coagulation item detection system according to claim 6, characterized in that: The lower shell is provided with an anti-slip strip at the position of the detection and heating module; the upper surface of the card slot is provided with a plurality of inner grooves recessed along its thickness direction; a total of 4 inserts are provided on both sides of the card slot, and accordingly, the number of insert slots of the heating plate card slot is 4.

14. The multi-index coagulation item detection system according to claim 11, characterized in that: The temperature sensor 1 in the detection and heating module is unidirectionally connected to the CPU.

15. The multi-index coagulation item detection system according to claim 11, characterized in that: The test card has five coagulation index tests, and accordingly, the test card is bidirectionally connected to the CPU through the electrode acquisition amplifier module in a five-way channel.

16. The multi-index coagulation item detection system according to claim 11, characterized in that: The main board also includes a base connector and a printer module. The base connector is used to connect a printer or charge, and the printer module is bidirectionally connected to the CPU.

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