Preparation method and application of pollen and carotenoid composition

Abstract

The invention discloses a preparation method and application of a pollen and carotenoid composition. The composition is prepared from the following raw materials in parts by weight: 100-10000 parts of bee pollen and 0.1-100 parts of carotenoid, and carotenoid micro-capsule powder is prepared from carotenoid crystals through food colloid and the like at the temperature of 50-70 DEG C. The bee pollen and the carotenoid microcapsule powder can be directly mixed to prepare products such as solid beverages and hard capsules; or adding a 5-10% povidone solution into the bee pollen, granulating, blending with the microcapsule powder raw material and the auxiliary material, and preparing tablets under the pressure of 5-15KN. The product prepared by the invention is greatly improved in related storage and delivery stability in common application of carotenoid series raw materials and pollen raw materials, effectively improves and expands application scenes and synergistic interaction functions of the two functional raw materials, and is suitable for industrial production and function creation application of the two related raw materials.

Description

Technical Field

[0001] The invention relates to the technical field of food and food processing, and more particularly to a preparation method and application of a pollen and carotenoid composition. Background Art

[0002] Pollen is a traditional healthy functional food material in my country. It contains rich functional substances, including proteins, vitamins, flavonoids, etc. In addition, it also contains biologically active ingredients such as biological enzymes, which can regulate the body's various functions, balance nutrition in the body, and enhance metabolism. It is an excellent raw material for the development of functional products. Carotenoids are a general term for a large class of natural pigments composed of isoprene units. They have bright colors such as yellow, orange, and red. They are the main source of vitamin A in the body. They also have antioxidant, immunomodulatory, anti-cancer, and anti-aging effects. However, the presence of its conjugated double bond structure is easily inactivated by light, heat, oxygen, etc., which greatly affects its processing and nutritional value.

[0003] In actual applications, it is found that after pollen, especially pollen raw materials after wall breaking, are mixed with carotenoid raw materials, based on the possible existence of pollen active enzymes, metal ions and other related effects, carotenoid functional components will show a significant acceleration of degradation trend, and the degradation process will be further deepened after tableting. At present, ordinary microencapsulation embedding pays more attention to water dispersibility and particle fineness. The stability in the state of a single powder meets the requirements. After contact with pollen and other raw materials that affect stability, it still has a phenomenon of promoting degradation of carotenoids after microencapsulation. Under the tableting preparation process, there are even phenomena such as microcapsule rupture, pigment dissolution, and tablet staining. There is no obvious improvement for the deterioration phenomenon of such contact powder and tablet application, which greatly reduces the application value of both. CN201911087043.7 For the processing of carotenoid raw materials, oils and fats are added to increase the dispersibility of carotenoid raw materials, and more quaternary ammonium compounds are added. The presence of oils and fats affects the strength of microcapsule powder particles and is prone to microcapsule rupture and oil dissolution under external forces. Although the presence of some synthetic reagent raw materials improves the processing characteristics, consumers will put forward more processing raw material application requirements under the current clean label requirements.

[0004] Therefore, in view of the phenomenon that carotenoid functional raw materials are easily degraded and lost, and the degradation trend increases significantly after contact and blending with sensitive raw materials such as pollen, the present invention proposes a solution for carotenoid microencapsulation, which has the appeal of being green, efficient, highly acceptable to ingredients, and highly industrially feasible, so as to improve the quality of the joint application of carotenoids and sensitive degradable raw materials such as pollen, increase the application scenarios, and achieve synergistic and other health functional benefits. Summary of the invention

[0005] In view of this, the present invention provides a preparation method and application of a pollen and carotenoid composition. The product prepared by the present invention has greatly improved storage and delivery stability in the joint application of carotenoid series raw materials and pollen raw materials, effectively improves and expands the application scenarios and synergistic functions of the two functional raw materials, and is suitable for the industrial production and functional creation application of the two related raw materials.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A method for preparing a pollen and carotenoid composition comprises the following steps:

[0008] (1) preparing carotenoid crystals through food colloids and functional components at 50-70° C. to obtain carotenoid microcapsule powder;

[0009] (2) directly mixing bee pollen with carotenoid microcapsule powder to prepare solid beverages, hard capsules and other products;

[0010] or,

[0011] 0.1-5% of povidone is added to bee pollen for granulation, and then the mixture is blended with carotenoid microcapsule powder and auxiliary materials, and tablets are obtained by compression.

[0012] Preferably, the pollen and carotenoid composition comprises, by weight: 100-10000 parts of bee pollen and 0.1-100 parts of carotenoids.

[0013] More preferably, the pollen and carotenoid composition comprises, by weight: 200-5000 parts of bee pollen and 1-80 parts of carotenoids.

[0014] Preferably, the carotenoid crystals include one or more of β-carotene, lutein, lycopene and zeaxanthin.

[0015] Preferably, the food colloid comprises one or more of gum arabic, flaxseed gum, alginate and chia seed gum.

[0016] Preferably, the functional ingredients include plant proteins and / or plant phenols and / or modified polysaccharides.

[0017] Preferably, the plant protein includes soy protein, flax protein, sesame protein, pea protein-related beans and oil protein; the plant phenols include seabuckthorn flavonoids, flax lignans, rapeseed polyphenol-related flavonoids, phenolic acids, and lignin phenolic components; the modified polysaccharides include water-soluble resistant cellulose, resistant starch, cyclodextrin, low-substituted hydroxypropyl cellulose, and carboxymethyl cellulose calcium.

[0018] Preferably, the preparation in step (1) is performed by spray granulation at 50-70° C. and 10-25 bar.

[0019] More preferably, the preparation in step (1) is performed by spray granulation at 50-60° C. and 15-20 bar.

[0020] Preferably, the particle size of the carotenoid microcapsule powder is 0.15-0.85 mm.

[0021] More preferably, the particle size of the carotenoid microcapsule powder is 0.25-0.425 mm.

[0022] Preferably, the granulation in step (3) is to prepare povidone into a 5-10% aqueous solution, and then add bee pollen for granulation, and the granulation temperature is 40-60°C.

[0023] More preferably, the granulation temperature is 45-50°C.

[0024] Preferably, the auxiliary materials in step (3) include one or more of microcrystalline cellulose, modified polysaccharides, magnesium stearate, and silicon dioxide, and the pressing pressure is 5-15 KN.

[0025] More preferably, the pressing pressure is 6-12 KN.

[0026] Another object of the present invention is to provide the use of the product obtained by the preparation method of the above-mentioned pollen and carotenoid composition in food and medicine.

[0027] It can be seen from the above technical solutions that, compared with the prior art, the present invention has the following technical effects:

[0028] The present invention aims at the limited application situation that even if carotenoid raw materials are contacted with pro-oxidative degradation functional raw materials such as pollen after general microencapsulation preparation, there is still no obvious improvement and accelerated degradation will occur. The present invention uses plant gum, plant protein, plant polyphenols to exert processing characteristics such as emulsification, anti-oxidation, and physical embedding to directly provide physical and chemical related comprehensive protection for carotenoid raw materials. There are no consumer-sensitive raw materials such as solvents and quaternary ammonium salts. The process can achieve complete industrial production at a relatively low temperature. It is green and efficient, and the production process conditions are friendly to sensitive raw materials such as light, heat, and oxygen.

[0029] The present invention uses plant glue wall materials and does not use plant oil for solubilization and dispersion, thereby increasing the strength of microcapsule particles and reducing the influence of oil dissolution on carotenoid raw materials. In addition, the particle size of the finished microcapsule particles is increased while balancing the application characteristics of the particles to meet normal solid application scenarios, further reducing the external contact of carotenoid microcapsule powder and improving its physical and chemical stability and particle pressure stress stability.

[0030] Through the optimal formula, it is determined that the use of povidone meets the tablet production requirements for pollen tablet processing at a lower pressure. The pressure control can effectively reduce the damage to the integrity of the microcapsule particles caused by the tableting pressure, and greatly reduce the impact of pollen on the continuous degradation of carotenoid raw materials during storage after tablet processing. This forms a green, efficient, and stable application and solution for the blending of pollen, a raw material that is sensitive to oxidative degradation, and carotenoid raw materials, which increases the application scenarios of the two and enhances their synergistic health benefits. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1

[0033] This embodiment provides a method for preparing a pollen and carotenoid composition, comprising the following steps:

[0034] (1) 10 parts of β-carotene crystal raw material, 40 parts of gum arabic, 30 parts of flaxseed gum, 10 parts of soy protein, 10 parts of water-soluble resistant cellulose, and 0.5 parts of flaxseed extract were dissolved in water to form a suspension, and spray granulated at 55° C. and 18 bar to form microcapsule particles (particle size distribution of 0.15-0.425 mm);

[0035] (2) 500 parts of broken rapeseed pollen and 100 parts of the microcapsule particles from (1) are mixed to obtain a mixture;

[0036] (3) 600 parts of the mixture in (2), 500 parts of inulin, 200 parts of blueberry powder, 10 parts of phospholipids, and 5 parts of silicon dioxide are mixed to form a solid beverage;

[0037] (4) 300 parts of the mixture in (2), 600 parts of resistant starch, 50 parts of microcrystalline cellulose and 50 parts of magnesium stearate are mixed and filled into hard capsules.

[0038] Example 2

[0039] This embodiment provides a method for preparing a pollen and carotenoid composition, comprising the following steps:

[0040] (1) 10 parts of lycopene crystal raw material, 25 parts of water-soluble resistant cellulose, 20 parts of flaxseed gum, 20 parts of alginate, 10 parts of gum arabic, 5 parts of flax protein, and 0.1 parts of lignan powder were dissolved in water to form a suspension, and spray granulated at 56° C. and 16 bar to form microcapsule particles (particle size distribution of 0.18-0.425 mm);

[0041] (2) adding 25 parts of 8% concentration of povidone to 100 parts of wall-broken rapeseed pollen at 46° C. for granulation;

[0042] (3) 500 parts of the rapeseed pollen granulated powder (2) and 100 parts of the microcapsule powder (1) are mixed and ground into powder;

[0043] (4) 600 parts of the mixed powder of (3) above, 250 parts of microcrystalline cellulose, 100 parts of low-substituted hydroxypropyl cellulose, 25 parts of silicon dioxide, and 25 parts of magnesium stearate are sieved and compressed into tablets under a pressure of 8 kN.

[0044] Example 3

[0045] This embodiment provides a method for preparing a pollen and carotenoid composition, comprising the following steps:

[0046] (1) 10 parts of lutein crystal raw material, 30 parts of alginate, 25 parts of resistant starch, 10 parts of flaxseed gum, 10 parts of gum arabic, 5 parts of flax protein, and 0.1 parts of seabuckthorn flavonoids were dissolved in water to form a suspension, and spray granulated at 50° C. and 20 bar to form microcapsule particles (particle size distribution of 0.18-0.425 mm);

[0047] (2) adding 25 parts of 10% concentration of povidone to 100 parts of broken pine pollen and granulating at 48° C.

[0048] (3) 500 parts of the rapeseed pollen granulated powder (2) and 100 parts of the microcapsule powder (1) are mixed and ground into powder;

[0049] (4) 600 parts of the mixed powder of (3) above, 250 parts of microcrystalline cellulose, 100 parts of cyclodextrin, 25 parts of silicon dioxide, and 25 parts of magnesium stearate are sieved and compressed into tablets under a pressure of 10 kN.

[0050] Example 4

[0051] This embodiment provides a method for preparing a pollen and carotenoid composition, comprising the following steps:

[0052] (1) Take 5 parts of β-carotene crystal raw material, 5 parts of zeaxanthin crystal, 25 parts of water-soluble resistant cellulose, 25 parts of flaxseed gum, 20 parts of alginate, 10 parts of gum arabic, 10 parts of phospholipids, and 0.1 parts of lignan powder, dissolve in water to form a suspension, and spray granulate at 58° C. and 15 bar to form microcapsule particles (particle size distribution of 0.15-0.25 mm);

[0053] (2) mixing 300 parts of wall-broken pine pollen, 200 parts of wall-broken rapeseed pollen and 100 parts of the microcapsule particles of (1);

[0054] (3) 600 parts of the mixture in (2), 300 parts of stachyose, 200 parts of inulin, 200 parts of citrus fruit powder, 10 parts of phospholipids and 5 parts of silicon dioxide are mixed to prepare a solid beverage;

[0055] (4) 300 parts of the mixture in (2), 600 parts of resistant starch, 50 parts of microcrystalline cellulose and 50 parts of magnesium stearate are mixed and filled into hard capsules.

[0056] Comparison of processing characteristics of prepared compositions

[0057] Comparative Example 1

[0058] In implementation case 2, the povidone solution was replaced with an equal amount of water, and the remaining proportions were the same as those in Example 2.

[0059] Comparative Example 2

[0060] In implementation case 2, the povidone solution was adjusted to 2%, and the other proportions were the same as those in embodiment 2.

[0061] Comparative Example 3

[0062] In implementation case 2, the povidone solution was adjusted to a 2% hydroxypropyl methylcellulose solution, and the other proportions were the same as those in Example 2.

[0063] Comparative Example 4

[0064] In implementation case 2, the povidone solution was adjusted to 8% hydroxypropyl methylcellulose solution, and the other proportions were the same as those in Example 2.

[0065] Comparative Example 5

[0066] In Example 3, the povidone solution was adjusted to 8% hydroxypropyl methylcellulose solution, and the other proportions were the same as those in Example 2;

[0067] The tableting hardness and other qualities were investigated by using different tableting pressures and different adhesives. The results are shown in Table 1.

[0068] Table 1 Application characteristics comparison

[0069]

[0070]

[0071] Note: “—” means unformed or undetectable

[0072] As can be seen from the above table, pollen raw materials are difficult to tablet, especially under lower tableting pressure conditions. By screening povidone, the quality of pollen tablets can be significantly improved compared to hydroxypropyl methylcellulose under the same pressure conditions, and tablets of ideal quality can be obtained under lower industrial tableting pressure conditions.

[0073] Comparison of the stability of the prepared compositions

[0074] Comparative Example 6

[0075] Commercially available β-carotene microcapsule powder, the wall material is mainly modified starch, phospholipids, edible oil, ascorbic acid, 10% carotene content

[0076] Comparative Example 7

[0077] The wall materials of commercially available lycopene microcapsule powder are mainly modified starch, phospholipids, edible oil, and ascorbic acid, with a 10% lycopene content.

[0078] Comparative Example 8

[0079] The commercially available lycopene microcapsule powder has a wall material mainly composed of gelatin, modified starch, mixed tocopherols, and polyglycerol fatty acid esters, with a 10% lycopene content.

[0080] Comparative Example 9

[0081] Lycopene microcapsule powder (particle size distribution < 0.15 mm) prepared by the same process and ingredients as in Example 2 after screening

[0082] The raw materials were sealed in bottles and stored at 37°C and 75% humidity. Samples were taken at 0, 10, 20, and 30 days to examine the retention percentage of the effective detection value of the carotenoid components compared with the initial value. The digestive bioaccessibility was compared with each evaluation sample using the INFOGEST static in vitro simulation system, and the retention ratio of the effective component content compared with the initial value after simulating digestion into the intestine was characterized. The results are shown in Table 2.

[0083] Table 2 Storage and delivery performance of powdered carotenoids

[0084]

[0085] Note: The above sample state microcapsule powder is the pure carotenoid microcapsule powder in each case, the pollen blend is the blend of microcapsule powder and pollen, and "—" means not evaluated

[0086] Comparative Examples 6-9 in the above table are all relatively fine powders, and it can be observed from the results that the storage performance of the microcapsule powders of each comparative example alone is still good, but after contacting and blending with pollen, a relatively obvious degradation trend is shown, while the samples of Implementation Examples 1-3 still maintain a high stability performance after blending with pollen, and the simulated digestion delivery stability of Implementation Example 2 is better than that of each comparative example. The above results show that the implementation cases of the present invention can significantly improve the effect of pollen raw materials on the oxidative degradation of carotenoid components, and appropriately increasing the particle size of carotenoid microcapsule powder is beneficial to its storage and delivery stability.

[0087] Table 3 Carotenoid storage and delivery performance after tableting

[0088]

[0089] Note: The above samples are prepared under different pressures with the same formula in each case.

[0090] Among the above samples, it can be observed that the carotenoid components of comparative examples 7-9 have certain precipitation and blurring of the tablets after tableting, and it is more obvious under 30KN conditions than under 10KN. For the sample of implementation case 2, it was preliminarily found that the carotenoid components had certain precipitation when the tablets prepared at 30KN were accelerated stored for 30 days. In each comparative example and embodiment, the tablets prepared at 30KN have a greater impact on the storage and delivery stability of the carotenoid components, and only the tablets prepared at 10KN in implementation case 2 performed better. The above results show that the present invention can significantly improve the application of carotenoid pollen tablets in dosage forms. Through the application of povidone, suitable wall materials and particle sizes, and the combined application of tableting processes, the stability of the pollen and carotenoid tablet development scenarios can be effectively improved, and the synergistic effect of its nutritional efficacy components can be ensured.

[0091] The comparison results in Tables 1-3 show that the composition process of the present invention can effectively improve the processing characteristics of the target raw materials, and can greatly improve the storage and delivery-related performance of the perishable functional ingredients, significantly improve their processing and nutritional value, and is easy to realize industrialization, with good health and social benefits.

[0092] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0093] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a pollen and carotenoid composition, characterized in that: The following steps are involved: (1) preparing carotenoid crystals through food colloids and functional components at 50-70° C. to obtain carotenoid microcapsule powder; (2) directly mixing bee pollen with carotenoid microcapsule powder and then preparing a powder type product; or, Povidone is added to bee pollen for granulation, and then the mixture is blended with carotenoid microcapsule powder and auxiliary materials, and a solid product is obtained by compression.

2. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The pollen and carotenoid composition comprises, by weight: 100-10000 parts of bee pollen and 0.1-100 parts of carotenoid crystals.

3. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The carotenoid crystals include one or more of β-carotene, lutein, lycopene and zeaxanthin.

4. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The food colloid comprises one or more of gum arabic, flaxseed gum, algae gum and chia seed gum.

5. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The functional ingredients include plant proteins and / or plant phenols and / or modified polysaccharides.

6. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The preparation in step (1) is performed by spray granulation at 50-70° C. and 10-25 bar.

7. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The particle size of the carotenoid microcapsule powder is 0.15-0.85 mm.

8. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The granulation in step (3) is to prepare polyvidone into a 5-10% (m / m) aqueous solution, and then add bee pollen for granulation, the polyvidone aqueous solution accounts for 0.1-5% of the mass of the bee pollen, and the granulation temperature is 40-60°C.

9. The method for preparing a pollen and carotenoid composition according to claim 1, characterized in that: The auxiliary materials in step (3) include one or more of microcrystalline cellulose, modified polysaccharides, magnesium stearate, and silicon dioxide, and the pressing pressure is 5-15KN.

10. Use of a product obtained by the method for preparing the pollen and carotenoid composition according to any one of claims 1 to 9 in food and medicine.

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