Resveratrol liposome applied to liquid preparation product and preparation method of resveratrol liposome

Abstract

The invention discloses a resveratrol liposome applied to a liquid preparation product and a preparation method of the resveratrol liposome. The resveratrol liposome is prepared from resveratrol, high-water-absorption protein, cyclodextrin and phospholipid. In the liposome, the mass percentage content of resveratrol is less than or equal to 40% and greater than or equal to 10%, the mass percentage content of high-water-absorption protein is greater than 15%, the mass percentage content of cyclodextrin is greater than or equal to 10%, and the mass percentage content of phospholipid component is less than or equal to 2% and greater than or equal to 0.5%. Compared with the prior art, the preparation method has the advantages that the technological process is green, simple and easy to operate, an ethanol solvent is not needed, the product yield is high, continuous production can be realized, and the prepared finished product is relatively high in dispersity in water and relatively high in product stability.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and in particular relates to a resveratrol liposome used in a liquid preparation product and a preparation method thereof. Background Art

[0002] Resveratrol is a polyphenol compound widely found in plants such as Polygonum cuspidatum, grapes, peanuts, and mulberries. It is regarded as another natural anticancer drug after paclitaxel. Resveratrol has biological activities such as anticancer, antioxidation, promoting wound healing, and alleviating neuralgia. However, the poor dispersibility and stability of resveratrol in water and its easy decomposition in light limit its application. At present, there are many documents introducing the preparation of resveratrol microcapsule powder, but most of them need to introduce solvent ethanol, and the operation process is complicated and cannot be mass-produced, so that it cannot be widely used. Therefore, it is necessary to adopt new formulas and processes to enable it to be mass-produced under the premise of solving the application defects of resveratrol. Summary of the invention

[0003] The purpose of the present invention is to provide a resveratrol liposome used in a liquid preparation product and a preparation method in order to solve the deficiencies of the prior art.

[0004] The purpose of the present invention is achieved by the following technical solutions:

[0005] A resveratrol liposome used in a liquid preparation product, prepared from resveratrol, highly water-absorbent protein, cyclodextrin and phospholipid;

[0006] In the liposome, the mass percentage of resveratrol is ≤40% and ≥10%, the mass percentage of the highly water-absorbent protein is >15%, the mass percentage of the cyclodextrin is ≥10%, and the mass percentage of the phospholipid component is ≤2% and ≥0.5%.

[0007] Preferably, in the liposome, the mass percentage of the highly water-absorbent protein is ≤55%.

[0008] Preferably, the mass percentage of the cyclodextrin is ≤55%.

[0009] Preferably, the water absorption rate of the highly water-absorbent protein is 20 to 100 g water / 10 g protein.

[0010] Preferably, the highly water-absorbent protein is plant protein.

[0011] Preferably, the highly water-absorbent protein is one or more selected from soy protein isolate and pea protein.

[0012] The method for preparing the resveratrol liposomes used in the liquid preparation product as described above comprises the following steps:

[0013] S1. Evenly mix the highly absorbent protein and resveratrol;

[0014] S2. adding water to phospholipids and cyclodextrin to prepare a wall material solution, adding a uniformly mixed highly absorbent protein and resveratrol, and then high-speed shearing to form a uniform liquid phase;

[0015] S3. Perform spray drying to obtain resveratrol liposomes.

[0016] Preferably, the solid content by mass in the liquid phase in step S2 is 30-40%.

[0017] Preferably, the shear rate in step S2 is 1500-2500 r / min.

[0018] Preferably, the spray drying parameters are:

[0019] The inlet air temperature is 160~180℃ and the outlet air temperature is 90~100℃.

[0020] Compared with the prior art, the process of the present application is green, simple and easy to operate, does not require the use of ethanol solvent, has a high product yield and can be produced continuously, and the prepared finished product has a relatively high dispersibility in water and a strong product stability. DETAILED DESCRIPTION

[0021] Currently, there are public technologies for preparing resveratrol liposomes using microencapsulation technology, but some of the preparation processes require the addition of ethanol, while others require multiple steps of processing, and none of them can be mass-produced.

[0022] The present invention increases the water dispersibility of liposome resveratrol by using phospholipids and β-cyclodextrin as external wall materials. In addition, highly water-absorbent protein (such as soy protein isolate, pea protein and other plant proteins) is introduced as the inner core wall material to form a uniform Pickering emulsion, which is then dried to prepare resveratrol liposomes with strong stability and good water solubility. Compared with conventional resveratrol microcapsules, this method has the advantages of high yield, green, environmental protection and continuous production. The specific description is as follows:

[0023] The present invention provides a resveratrol liposome used in a liquid preparation product, which comprises, by weight, resveratrol, highly water-absorbent protein, cyclodextrin and phospholipid;

[0024] In the liposome, the mass percentage of resveratrol is ≤40% and ≥10%, the mass percentage of highly water-absorbent protein is >15%, the mass percentage of cyclodextrin is ≥10%, and the mass percentage of phospholipid components is ≤2% and ≥0.5%.

[0025] Among them, highly absorbent protein has strong water absorption. As the internal core wall material, it is mixed with resveratrol. After seeing water, the highly absorbent protein can quickly disperse into the water, absorb a large amount of water, and form a primary barrier outside the resveratrol, making the solution thicker and suspended without rapid precipitation, which significantly enhances the dispersibility and stability of resveratrol in water.

[0026] Cyclodextrin and phospholipids are used as external wall materials. Phospholipids act as a bilayer membrane to wrap the material, which can prevent resveratrol from being released and increase the stability of the membrane. In addition, phospholipids act as emulsifiers to enhance the suspension and dispersibility of the material. Cyclodextrin, as a macromolecular material with a cavity, can combine well with phospholipids, modify phospholipids, and protect their stability. Adding cyclodextrin and phospholipids can form a second-level barrier outside resveratrol, further enhancing the dispersibility and stability of resveratrol in water.

[0027] The cyclodextrin is preferably β-cyclodextrin, which has better embedding properties than other dextrins such as α-cyclodextrin and γ-cyclodextrin.

[0028] Experimental verification shows that when the amount of highly absorbent protein and cyclodextrin is too small, the dispersibility and stability of resveratrol in water will be seriously affected. When the amount of phospholipids is too much, the viscosity of the liquid will increase and the fluidity of the product will be reduced. A large amount of material will adhere to the equipment, which will seriously affect the product yield. When the amount of phospholipids is too low, the material sedimentation speed is faster and the product stability is reduced.

[0029] The resveratrol content in liposomes should also be appropriate. When the content is too high, the amount of wall material used will be insufficient and resveratrol cannot be fully encapsulated. When the content is too low, the drug loading capacity of the liposomes will be too low, resulting in a waste of resources.

[0030] In the prior art, the preparation of liposome products requires the introduction of ethanol for preparation. The present application uses less phospholipids and more water (herein, the proportion of phospholipids in water is relatively low during the preparation process) to form the liposome bilayer, which can avoid the introduction of ethanol solvent.

[0031] Preferably, in order to increase the content of resveratrol in the liposome, the mass percentage of the highly water-absorbing protein in the liposome is ≤55%, and the mass percentage of the cyclodextrin is ≤55%.

[0032] When the water absorption rate is too high, the feed liquid will be very viscous and production cannot be carried out. When the water absorption rate is too low, it will not achieve the desired effect. Therefore, the water absorption rate of highly absorbent protein is preferably 20-100g water / 10g protein.

[0033] Compared with animal protein, plant protein has lower calories, cholesterol and saturated fat, and contains special health-care ingredients, such as soy isoflavones with estrogen-like effects, plant sterols with lipid-lowering effects, polyphenol antioxidants with anti-cancer and anti-aging effects, etc. The use of plant protein can effectively reduce the risk of chronic diseases while obtaining high-quality protein, which is of great significance to human health; in addition, plant protein does not contain lactose and will not cause gastrointestinal discomfort, etc. Therefore, preferably, the highly absorbent protein is plant protein, such as soy protein isolate and pea protein.

[0034] The present application also provides a method for preparing resveratrol liposomes used in liquid preparation products, comprising the following steps:

[0035] S1. Evenly mix the highly absorbent protein and resveratrol;

[0036] S2. Add water to phospholipids and cyclodextrin to prepare a wall material solution, then add a well-mixed highly absorbent protein and resveratrol, and then stir and shear at high speed to form a uniform liquid phase; to ensure uniform shearing, the shear rate is preferably 1500 to 2500 r / min, and the mass percentage of solids in the liquid phase is preferably 30 to 40%;

[0037] S3. Then spray drying is performed to obtain resveratrol liposomes. The spray drying parameters are preferably: inlet air temperature 160-180°C, outlet air temperature 90-100°C.

[0038] The present application uses highly water-absorbent protein (protein can significantly thicken and suspend after being dispersed in water, and does not quickly precipitate) as the internal core wall material, which is first mixed evenly with resveratrol, and then added to the external wall material solution formed by phospholipids and cyclodextrin. The protein quickly absorbs water to form a primary barrier, and after being dispersed by stirring and shearing, a uniform liquid phase is formed. The phospholipids and cyclodextrin form a second barrier, and the final product, resveratrol liposomes, is obtained by spray drying.

[0039] Compared with the prior art, the process of the present application is green, simple and easy to operate, does not require the use of ethanol solvent, has a high product yield and can be produced continuously, and the prepared finished product has a relatively high dispersibility in water and a strong product stability.

[0040] Example 1

[0041] The formula of resveratrol liposome is: by weight percentage, resveratrol 40%, highly water-absorbent protein 40%, β-cyclodextrin 19% and phospholipid 1%; the highly water-absorbent protein is soy protein isolate with a water absorption rate of 50g water / 10g protein.

[0042] The above raw materials were used to prepare resveratrol liposomes according to the following method.

[0043] S1. Evenly mix the highly absorbent protein and resveratrol;

[0044] S2. Add water to phospholipids and cyclodextrin to prepare a wall material solution, add a well-mixed highly absorbent protein and resveratrol, and then stir and shear at a high speed, wherein the stirring rate is 50 r / min, the stirring time is 10 min, the shear rate is 2000 r / min, and the shear time is 5 min to form a uniform liquid phase; the solid mass percentage in the liquid phase is 35%;

[0045] S3. Finally, spray drying is performed to obtain resveratrol liposomes. The spray drying air inlet temperature is 170°C and the air outlet temperature is 95°C.

[0046] Examples 2 to 5 and Comparative Examples 1 to 4 used different formulas to prepare different resveratrol liposome samples, statistically analyzed the product yields, and sampled the above samples for accelerated tests and sedimentation tests. The formula compositions and test results of Examples 2 to 5 and Comparative Examples 1 to 4 are shown in Table 1.

[0047] The accelerated test conditions are: temperature 37°C, humidity 75%, time 3 months, and the detection index is product whiteness. The smaller the change in product whiteness, the better the product stability.

[0048] The sedimentation test method is: take 5g of resveratrol liposome sample and add it into 50ml of room temperature water, stir and disperse it evenly, let it stand and measure the sedimentation ratio. The sedimentation ratio refers to the ratio of the lower layer volume after sedimentation to the initial volume. The larger the sedimentation ratio, the less the upper clear liquid precipitates, and the better the product stability.

[0049] Table 1

[0050]

[0051]

[0052] Through the above data analysis, when the protein addition amount is greater than 15%, β-cyclodextrin is greater than or equal to 10%, and the phospholipid component is less than or equal to 2% and greater than or equal to 0.5%, the yield of the prepared resveratrol microcapsule powder can be above 90%, can be stably dispersed in water, and has relatively high stability under accelerated conditions. When the amount of highly hygroscopic protein is too small, such as in Comparative Example 2, the whiteness of the product is significantly reduced, and the sedimentation is relatively low, there is a flocculent precipitate, and the stability is poor. When the amount of β-cyclodextrin is too small, such as in Comparative Example 1, the whiteness of the product is also significantly reduced, and there is obvious agglomeration in water, and the dispersibility and stability are poor. When phospholipids are not used, the sedimentation ratio is very low, the sedimentation rate is too fast, and the product stability is poor (such as Comparative Example 4). When the amount of phospholipids is too much, such as in Comparative Example 3, the product yield is reduced (such as Comparative Example 3).

[0053] Although preferred embodiments of the present invention have been described, additional changes and modifications may be made to these embodiments by those skilled in the art once the basic inventive concepts are known. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention. Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A resveratrol liposome used in a liquid preparation product, characterized in that: It is prepared from resveratrol, highly absorbent protein, cyclodextrin and phospholipids; In the liposome, the mass percentage of resveratrol is ≤40% and ≥10%, the mass percentage of the highly water-absorbent protein is >15%, the mass percentage of the cyclodextrin is ≥10%, and the mass percentage of the phospholipid component is ≤2% and ≥0.5%.

2. The resveratrol liposome used in a liquid preparation product according to claim 1, characterized in that: In the liposome, the mass percentage of the highly water-absorbent protein is ≤55%.

3. The resveratrol liposome used in a liquid preparation product according to claim 1, characterized in that: The mass percentage of the cyclodextrin is ≤55%.

4. The resveratrol liposome used in a liquid preparation product according to claim 1, characterized in that: The water absorption rate of the highly water-absorbent protein is 20 to 100 g water / 10 g protein.

5. The resveratrol liposome used in a liquid preparation product according to claim 1, characterized in that: The highly water-absorbent protein is plant protein.

6. The resveratrol liposome used in a liquid preparation product according to claim 5, characterized in that: The highly water-absorbent protein is one or more selected from soy protein isolate and pea protein.

7. The method for preparing resveratrol liposomes for use in liquid preparation products according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Evenly mix the highly absorbent protein and resveratrol; S2. adding water to phospholipids and cyclodextrin to prepare a wall material solution, adding a uniformly mixed highly absorbent protein and resveratrol, and then high-speed shearing to form a uniform liquid phase; S3. Perform spray drying to obtain resveratrol liposomes.

8. The method for preparing resveratrol liposomes for use in liquid preparation products according to claim 7, characterized in that: The solid content by mass percentage in the liquid phase of step S2 is 30-40%.

9. The method for preparing resveratrol liposomes for use in liquid preparation products according to claim 7, characterized in that: The shear rate in step S2 is 1500-2500 r / min.

10. The method for preparing resveratrol liposomes for use in liquid preparation products according to claim 7, characterized in that: The spray drying parameters are: The inlet air temperature is 160~180℃ and the outlet air temperature is 90~100℃.

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