Method and equipment for establishing intervertebral disc degeneration animal model

Abstract

The invention relates to the field of animal model establishment, and discloses a method and equipment for establishing an intervertebral disc degeneration animal model.The method for establishing the intervertebral disc degeneration animal model.The method comprises the marking stage, specifically, an animal is processed, the processed animal is placed on a test bed, then a straight line is drawn in the trend of the spine spinous process of the animal, and the animal is marked; a long needle head perpendicular to the marking line is placed at the highest points of the iliac bones on the two sides of the animal, and a preliminary image is obtained; and fine adjustment processing: adjusting the position of the long needle head according to the initial image to enable the long needle head to coincide with the intervertebral disc position between the L5 and the L6, and then marking to obtain a second marking line. Through gradual implementation of a marking stage, fine adjustment treatment, an injection stage and subsequent treatment, establishment of the intervertebral disc degeneration animal model is realized, the whole process operation is relatively simple and easy to realize, the time required for constructing each model is relatively short, the cost is relatively low, and the method can be widely applied to various animal species; meanwhile, the damage to animals is small.

Description

Technical Field

[0001] The present invention relates to the technical field of establishing animal models, and in particular to a method and equipment for establishing an intervertebral disc degeneration animal model. Background Art

[0002] Animal model establishment refers to the use of experimental animals for scientific research. These animals need to undergo artificial breeding, physiological indicator measurement, and drug testing to verify the efficacy and safety of human drugs, therapies, etc. Usually, different types of animals are selected for experiments based on the research purpose and required results, such as mice, rats, rabbits, monkeys, etc. They are mainly divided into four types: cell culture models, animal experimental models, specimen models, and cell line models.

[0003] The establishment of traditional animal models of intervertebral disc degeneration is usually carried out through four methods, namely, percutaneous annulus puncture under CT guidance, surgical exposure of the annulus puncture, mechanical pressure method, and gene knockout model. When establishing the intervertebral disc degeneration model, it is often expensive and time-consuming, which easily leads to waste of manpower. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a method and device for establishing an intervertebral disc degeneration animal model, which solves the problem that the establishment of the traditional intervertebral disc degeneration animal model is often expensive, time-consuming, and easily causes a waste of manpower.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: A method for establishing an animal model of intervertebral disc degeneration comprises the following steps: S1, marking stage: the animal is processed and placed on the test bench, and then a straight line is drawn along the spinous process of the animal's spine to obtain the first marking line. Then a long needle is placed perpendicular to the marking line at the highest point of the ilium on both sides of the animal, and the animal is photographed under X-ray to obtain a preliminary image; S2, fine-tuning: adjust the position of the long needle according to the preliminary image, so that the long needle coincides with the intervertebral disc between L5 and L6, and then mark it to obtain the second marking line, and then translate the intersection of the first marking line and the second marking line by a corresponding distance, mark it, and obtain the needle insertion point; S3, injection stage: insert the injection needle into the skin from the injection point until it touches the vertebral body or transverse process of the spine, and then adjust the injection angle and direction so that the injection needle falls on the center of the intervertebral disc when the animal is in the prone position and lateral position under the X-ray machine, and then use an 18G needle to slide left and right in the intervertebral disc. After the sliding is completed, inject the contrast agent; S4. Follow-up treatment: After the injection is completed, press the needle insertion point, then wipe it with iodine to complete the treatment, and inject analgesics and anti-inflammatory agents after the treatment is completed.

[0006] Preferably, the treatment in step S1 is to anesthetize the animal, and then remove the hair on its epidermis along the spinous process of its spine and clean it.

[0007] Preferably, the corresponding distance in step S2 is 4 cm to 5 cm to the left or right, and the number of left and right sliding in step S3 is 3 times, and the sliding range is 0.5 cm to 1 cm.

[0008] Preferably, the pressing time in step S4 is 14 minutes to 16 minutes, and the injection time of the analgesic and anti-inflammatory agent is 3 days to 5 days.

[0009] A device for establishing an animal model of intervertebral disc degeneration, comprising a test bench, wherein a first electric slide rail is arranged on the top of the test bench, a first slider is arranged inside the first electric slide rail, a movable plate is fixedly connected to the top of the first slider, a second electric slide rail is arranged on one side of the movable plate, a second slider is arranged inside the second electric slide rail, a mounting plate is fixedly connected to one side of the second slider, a groove is arranged on the bottom of the mounting plate, a connecting plate is fixedly connected to the top of the inner wall of the mounting plate, a first servo motor is fixedly connected to the top of the mounting plate, an output end of the first servo motor passes through the mounting plate and is fixedly connected to the top of the connecting plate, the outer wall of the first gear is evenly meshed with the second gear, the outer wall of the second gear is meshed with the inner gear ring, the outer wall of the inner gear ring is provided with a connecting structure, a medical X-ray machine is arranged at the bottom of the connecting structure, and an adjustment structure is arranged at the bottom of the test bench.

[0010] Preferably, the connecting structure includes a first annular connecting plate, the inner wall of which is fixedly connected to the circumferential outer wall of the inner gear ring, the outer wall of which is slidably connected to the inside of the groove, the bottom of which is fixedly connected to a second annular connecting plate, the bottom of which is fixedly connected to the top of a medical X-ray machine, and the medical X-ray machine is located below the mounting plate.

[0011] Preferably, the middle portion of the second gear is rotatably connected to the bottom of the connecting plate via a rotating shaft, and the inner gear ring is located inside the groove.

[0012] Preferably, the adjustment structure includes a base, a mounting block is symmetrically fixedly connected to the top of the base, a slide groove is arranged on the top of the mounting block, a second servo motor is fixedly connected to one side of the mounting block, an output end of the second servo motor passes through one side of the mounting block and is fixedly connected to a bidirectional screw rod, an outer wall of the bidirectional screw rod is symmetrically threadedly connected to a third slider, and an outer wall of the third slider is slidably connected to the inside of the slide groove.

[0013] Preferably, the top of the third slider is symmetrically fixedly connected with a first lug, a telescopic frame is provided on the side opposite to the first lug, a second lug is symmetrically provided on the top of the telescopic frame, the top of the second lug is fixedly connected with a connecting block, and the top of the connecting block is fixedly connected to the bottom of the test bench.

[0014] Working principle: When using this equipment, the operation of the first electric slide rail drives the first slider to move horizontally left and right, thereby driving the movable plate and the mounting plate to move horizontally left and right, thereby driving the connecting structure and the medical X-ray machine to move horizontally left and right, thereby adjusting the horizontal position of the medical X-ray machine according to needs; the operation of the second electric slide rail drives the second slider to move vertically up and down, thereby driving the mounting plate, the connecting structure, and the medical X-ray machine to move vertically up and down, thereby adjusting the height of the medical X-ray machine according to needs; the operation of the first servo motor drives the first gear to rotate, thereby driving the second gear to rotate, thereby driving the inner gear ring to rotate , thereby driving the first annular connecting plate to rotate inside the groove, thereby driving the second annular connecting plate to rotate, thereby driving the medical X-ray machine to rotate, so that the shooting angle of the medical X-ray machine can be adjusted according to the shooting requirements, and the first annular connecting plate is limited to a certain position through the groove to enhance its rotation stability. Through the operation of the second servo motor, the bidirectional lead screw is driven to rotate, thereby driving the third slider to move closer to or away from each other inside the slide groove, thereby driving the telescopic frame to operate, thereby driving the connecting block to rise or fall, thereby driving the test bench to rise or fall, so that the height of the test bench can be adjusted according to the height and usage requirements of the user.

[0015] The present invention provides a method and device for establishing an animal model of intervertebral disc degeneration. It has the following beneficial effects: 1. The present invention realizes the establishment of an animal model of intervertebral disc degeneration by gradually implementing the marking stage, fine-tuning treatment, injection stage, and subsequent treatment. The whole process is relatively simple and easy to implement. The time required for constructing each model is short and the cost is low. It can be widely used in various animal species. At the same time, it causes little damage to the animals and does not cause additional harm, thus solving the problem that the establishment of traditional animal models of intervertebral disc degeneration is often expensive, time-consuming, and prone to waste of manpower.

[0016] 2. The present invention drives the medical X-ray machine to move horizontally to the left and right through the operation of the first electric slide rail, so that the horizontal position of the medical X-ray machine can be adjusted according to needs. The medical X-ray machine is driven to move vertically up and down through the operation of the second electric slide rail, so that the height of the medical X-ray machine can be adjusted according to needs. The connection structure and the medical X-ray machine are driven to rotate through the operation of the first servo motor, so that the shooting angle of the medical X-ray machine can be adjusted according to shooting requirements, thereby enhancing the practicality of the device.

[0017] 3. The present invention drives the bidirectional screw to rotate through the operation of the second servo motor, thereby driving the third slider to move closer to or away from each other inside the slide slot, thereby driving the telescopic frame to operate, thereby driving the test bench to be raised or lowered, so that the height of the test bench can be adjusted according to the user's height and usage requirements, thereby further enhancing the practicality of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A flow chart of a method for establishing an intervertebral disc degeneration animal model proposed by the present invention; Figure 2 A schematic diagram of the three-dimensional structure of a device for establishing an intervertebral disc degeneration animal model proposed by the present invention; Figure 3 A schematic diagram of the internal structure of a mounting plate of a device for establishing an intervertebral disc degeneration animal model proposed by the present invention; Figure 4 This is a schematic diagram of the structure of the inner gear ring of a device for establishing an intervertebral disc degeneration animal model proposed by the present invention; Figure 5 This is a schematic diagram of the structure of the groove of a device for establishing an animal model of intervertebral disc degeneration proposed by the present invention; Figure 6 This is a schematic diagram of the structure of a bidirectional screw rod of a device for establishing an animal model of intervertebral disc degeneration proposed by the present invention; Figure 7 This is an X-ray image taken after the needle of the present invention punctures the intervertebral disc and injects contrast agent.

[0019] Among them, 1. test bench; 2. first electric slide rail; 3. first slider; 4. moving plate; 5. second electric slide rail; 6. second slider; 7. mounting plate; 8. medical X-ray machine; 9. first servo motor; 10. telescopic frame; 11. mounting block; 12. second servo motor; 13. base; 14. connecting block; 15. bidirectional screw rod; 16. third slider; 17. first lug; 18. second lug; 19. slide groove; 20. groove; 21. connecting plate; 22. first annular connecting plate; 23. inner gear ring; 24. first gear; 25. second annular connecting plate; 26. second gear. DETAILED DESCRIPTION

[0020] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Example: Please see attached Figure 1 The present invention provides a method for establishing an animal model of intervertebral disc degeneration, comprising the following steps: S1, marking stage: the animal is processed and placed on the test bench, and then a straight line is drawn along the spinous process of the animal's spine to obtain the first marking line. Then a long needle is placed perpendicular to the marking line at the highest point of the ilium on both sides of the animal, and the animal is photographed under X-ray to obtain a preliminary image; S2, fine-tuning: adjust the position of the long needle according to the preliminary image, so that the long needle coincides with the intervertebral disc between L5 and L6, and then mark it to obtain the second marking line, and then translate the intersection of the first marking line and the second marking line by a corresponding distance, mark it, and obtain the needle insertion point; S3, injection stage: insert the injection needle into the skin from the injection point until it touches the vertebral body or transverse process of the spine, and then adjust the injection angle and direction so that the injection needle falls on the center of the intervertebral disc when the animal is in the prone position and lateral position under the X-ray machine, and then use an 18G needle to slide left and right in the intervertebral disc. After the sliding is completed, inject the contrast agent; S4. Follow-up treatment: After the injection is completed, press the needle insertion point, then wipe it with iodine to complete the treatment, and inject analgesics and anti-inflammatory agents after the treatment is completed.

[0022] Specifically, the animal is processed and placed on a test bench, and then a straight line is drawn along the spinous process of the animal's spine to obtain a first marking line, which is then used to align and locate the key structure of the intervertebral disc, thereby helping to ensure the accuracy of the operation. Then, a long needle perpendicular to the marking line is placed at the highest point of the ilium on both sides of the animal, and the animal is photographed under X-ray to obtain a preliminary image, thereby allowing the operator to see the precise position of the animal's spine and intervertebral disc in real time, which is helpful for subsequent fine-tuning of the needle position; By adjusting the position of the long needle according to the preliminary image, the long needle is aligned with the intervertebral disc between L5 and L6, and then marking is performed to obtain the second marking line, thereby ensuring the accuracy of the injection operation and ensuring that the injection needle can accurately penetrate the target intervertebral disc. Then, the intersection of the first marking line and the second marking line is translated by a corresponding distance and marked to obtain the needle insertion point, thereby avoiding interference with other structures (such as spinal nerve roots, etc.) and ensuring that the injection is injected into the correct intervertebral disc position; The injection needle is inserted into the skin from the injection point until it touches the vertebral body or transverse process of the spine, and then the injection angle and direction are adjusted so that the injection needle falls on the center of the intervertebral disc when the animal is in the prone position and the lateral position under the X-ray machine, so as to ensure that the injection liquid or contrast agent enters the intervertebral disc, thereby simulating the degeneration or damage of the intervertebral disc. Then, an 18G needle is used to slide left and right in the intervertebral disc, and the contrast agent is injected after the sliding is completed. The injection of contrast agent not only helps to visualize the intervertebral disc structure, but also may cause certain chemical stimulation or damage to the inside of the intervertebral disc, providing the necessary conditions for simulating intervertebral disc degeneration. The sliding action can cause changes in the structure inside the intervertebral disc and promote the occurrence of intervertebral disc degeneration. After the injection is completed, pressure is applied to the injection site to help reduce bleeding and promote initial wound healing, and then iodine is wiped to ensure the sterility of the operating area and reduce the risk of infection. After the treatment is completed, analgesics and anti-inflammatory agents are injected to ensure the comfort of the animal and reduce complications caused by the operation; Through the gradual implementation of the marking stage, fine-tuning treatment, injection stage, and subsequent treatment, the establishment of an animal model of intervertebral disc degeneration is achieved, and the entire process is relatively simple and easy to implement. The construction of each model takes a short time and is low in cost, and can be widely used in various animal species. It also causes little damage to the animals and does not cause additional harm, thus solving the problem that the establishment of traditional animal models of intervertebral disc degeneration is often expensive, time-consuming, and prone to waste of manpower.

[0023] The treatment in step S1 is to anesthetize the animal, and then remove the hair on its epidermis along the direction of its spinous process and clean it; the corresponding distance in step S2 is 4 cm to 5 cm to the left or right, the number of left and right sliding in step S3 is 3 times, and the sliding range is 0.5 cm to 1 cm; the pressing time in step S4 is 14 minutes to 16 minutes, and the time for injecting analgesics and anti-inflammatory agents is 3 days to 5 days.

[0024] Specifically, the animal is anesthetized, and then the hair on its epidermis is removed and cleaned along the spinous process of its spine, so as to prevent the animal from feeling pain or discomfort during the experiment, and keep it in a stable and controllable state to facilitate subsequent operations. The precise injection position is determined by translating 4 cm to 5 cm to the left or right, and interference with other structures (such as spinal nerve roots, etc.) is avoided, while ensuring that the injection is injected into the correct intervertebral disc. The number of left and right sliding is 3 times, and the sliding range is 0.5 cm to 1 cm, so as to increase the damaged area of ​​the intervertebral disc, better simulate the situation of intervertebral disc degeneration, and can cause changes in the structure inside the intervertebral disc, promoting the occurrence of intervertebral disc degeneration. The pressing time is 14 minutes to 16 minutes, so as to reduce bleeding and prevent infection at the needle hole, and help promote the initial healing of the wound. The injection time of analgesics and anti-inflammatory agents is 3 days to 5 days, so as to relieve the pain of the animals, ensure the comfort of the animals, and reduce complications caused by operations.

[0025] Please see attached Figure 2 -Attached Figure 6 , a device for establishing an animal model of intervertebral disc degeneration, comprising a test bench 1, a first electric slide rail 2 is arranged on the top of the test bench 1, a first slider 3 is arranged inside the first electric slide rail 2, a moving plate 4 is fixedly connected to the top of the first slider 3, a second electric slide rail 5 is arranged on one side of the moving plate 4, a second slider 6 is arranged inside the second electric slide rail 5, a mounting plate 7 is fixedly connected to one side of the second slider 6, a groove 20 is arranged at the bottom of the mounting plate 7, a connecting plate 21 is fixedly connected to the top of the inner wall of the mounting plate 7, a first servo motor 9 is fixedly connected to the top of the mounting plate 7, an output end of the first servo motor 9 passes through the mounting plate 7 and is fixedly connected to the top of the connecting plate 21 with a first gear 24, an outer wall of the first gear 24 is evenly meshed with a second gear 26, an outer wall of the second gear 26 is meshed with an inner gear ring 23, a connecting structure is arranged on the outer wall of the inner gear ring 23, a medical X-ray machine 8 is arranged at the bottom of the connecting structure, and an adjustment structure is arranged at the bottom of the test bench 1.

[0026] Specifically, through the operation of the first electric slide rail 2, the first slider 3 is driven to move horizontally left and right, thereby driving the movable plate 4 and the mounting plate 7 to move horizontally left and right, thereby driving the connecting structure and the medical X-ray machine 8 to move horizontally left and right, so that the horizontal position of the medical X-ray machine 8 can be adjusted according to needs. Through the operation of the second electric slide rail 5, the second slider 6 is driven to move vertically up and down, thereby driving the mounting plate 7, the connecting structure, and the medical X-ray machine 8 to move vertically up and down, so that the height of the medical X-ray machine 8 can be adjusted according to needs. Through the operation of the first servo motor 9, the first gear 24 is driven to rotate, thereby driving the second gear 26 to rotate, thereby driving the inner gear ring 23 to rotate, thereby driving the connecting structure and the medical X-ray machine 8 to rotate, so that the shooting angle of the medical X-ray machine 8 can be adjusted according to shooting requirements, thereby enhancing the practicality of the device. Through the operation of the adjustment structure, the test bench 1 is driven to move vertically up and down, so that the height of the test bench 1 can be adjusted according to the height and usage requirements of the user, thereby further enhancing the practicality of the device.

[0027] Please see attached Figure 3 -Attached Figure 5 The connection structure includes a first annular connecting plate 22, the inner wall of the first annular connecting plate 22 is fixedly connected to the circumferential outer wall of the inner gear ring 23, the outer wall of the first annular connecting plate 22 is slidably connected to the inside of the groove 20, the bottom of the first annular connecting plate 22 is fixedly connected to the second annular connecting plate 25, the bottom of the second annular connecting plate 25 is fixedly connected to the top of the medical X-ray machine 8, and the medical X-ray machine 8 is located below the mounting plate 7; the middle part of the second gear 26 is rotatably connected to the bottom of the connecting plate 21 through a rotating shaft, and the inner gear ring 23 is located inside the groove 20.

[0028] Specifically, the inner wall of the first annular connecting plate 22 is connected to the outer wall of the inner gear ring 23, so that when the inner gear ring 23 rotates, the first annular connecting plate 22 can be driven to rotate inside the groove 20, thereby driving the second annular connecting plate 25 to rotate, thereby driving the medical X-ray machine 8 to rotate, thereby facilitating the adjustment of the angle of the medical X-ray machine 8, and the first annular connecting plate 22 is limited by the groove 20 to enhance its rotation stability.

[0029] Please see attached Figure 1 , Attachment Figure 6The adjustment structure includes a base 13, a mounting block 11 is symmetrically fixedly connected to the top of the base 13, a slide groove 19 is arranged on the top of the mounting block 11, a second servo motor 12 is fixedly connected to one side of the mounting block 11, an output end of the second servo motor 12 passes through one side of the mounting block 11 and is fixedly connected to a bidirectional lead screw 15, a third slider 16 is symmetrically threadedly connected to the outer wall of the bidirectional lead screw 15, and the outer wall of the third slider 16 is slidably connected to the inside of the slide groove 19; a first lug 17 is symmetrically fixedly connected to the top of the third slider 16, a telescopic frame 10 is arranged on the side opposite to the first lug 17, a second lug 18 is symmetrically arranged on the top of the telescopic frame 10, a connecting block 14 is fixedly connected to the top of the second lug 18, and the top of the connecting block 14 is fixedly connected to the bottom of the test bench 1.

[0030] Specifically, the operation of the second servo motor 12 drives the bidirectional screw rod 15 to rotate, thereby driving the third slider 16 to move closer to or away from each other inside the slide groove 19, thereby driving the telescopic frame 10 to operate, thereby driving the connecting block 14 to rise or fall, thereby driving the test bench 1 to rise or fall, so that the height of the test bench 1 can be adjusted according to the user's height and usage requirements.

[0031] The following is further introduced in conjunction with specific embodiments: Embodiment 1: Please see attached Figure 7 After the animal is anesthetized, it is placed prone on the test table under the X-ray machine. A straight line is drawn along the direction of the animal's spinous process as a mark. The highest point of the ilium on both sides of the animal is touched by hand. A long needle is placed perpendicular to the marked line and photographed under X-ray. If the needle is not between L5 and L6 under X-ray, fine-tune it until it is confirmed that the needle coincides with the intervertebral disc between L5 and L6. Mark it and make a mark about 4-5 cm to the left or right of the intersection of the two marked lines. This marked point is the needle insertion point.

[0032] Insert the injection needle into the skin from the injection point until there is resistance (generally the injection needle touches the vertebral body or transverse process of the spine at this time), slowly adjust the angle and direction of the needle until the needle falls into the intervertebral disc when the animal is in the prone and lateral positions under X-ray (this step is a key operation, and it is necessary to ensure that the needle falls in the center of the intervertebral disc in both photo positions). At this time, use an 18G needle to slide slightly left and right in the intervertebral disc 3 times, with a sliding range of 0.5-1 cm. After completion, slowly push the needle, press the injection point for about 15 minutes, wipe with iodine to disinfect, and inject analgesics and anti-inflammatory agents for 3-5 days after surgery.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for establishing an animal model of intervertebral disc degeneration, characterized in that: The following steps are involved: S1, marking stage: the animal is processed and placed on the test bench, and then a straight line is drawn along the spinous process of the animal's spine to obtain the first marking line. Then a long needle is placed perpendicular to the marking line at the highest point of the ilium on both sides of the animal, and the animal is photographed under X-ray to obtain a preliminary image; S2, fine-tuning: adjust the position of the long needle according to the preliminary image, so that the long needle coincides with the intervertebral disc between L5 and L6, and then mark it to obtain the second marking line, and then translate the intersection of the first marking line and the second marking line by a corresponding distance, mark it, and obtain the needle insertion point; S3, injection stage: insert the injection needle into the skin from the injection point until it touches the vertebral body or transverse process of the spine, and then adjust the injection angle and direction so that the injection needle falls on the center of the intervertebral disc when the animal is in the prone position and lateral position under the X-ray machine, and then use an 18G needle to slide left and right in the intervertebral disc. After the sliding is completed, inject the contrast agent; S4. Follow-up treatment: After the injection is completed, press the needle insertion point, then wipe it with iodine to complete the treatment, and inject analgesics and anti-inflammatory agents after the treatment is completed.

2. The method for establishing an animal model of intervertebral disc degeneration according to claim 1, characterized in that: The process in step S1 is to anesthetize the animal, and then remove the hair on its epidermis along the spinous process of its spine and clean it.

3. The method for establishing an animal model of intervertebral disc degeneration according to claim 1, characterized in that: The corresponding distance in step S2 is 4 cm to 5 cm to the left or right, and the number of left and right sliding in step S3 is 3 times, and the sliding range is 0.5 cm to 1 cm.

4. The method for establishing an animal model of intervertebral disc degeneration according to claim 1, characterized in that: The pressing time in step S4 is 14 minutes to 16 minutes, and the injection time of the analgesic and anti-inflammatory agent is 3 days to 5 days.

5. A device for establishing an animal model of intervertebral disc degeneration, characterized in that: The method for establishing a test bench as claimed in any one of claims 1 to 4 comprises a test bench (1), wherein a first electric slide rail (2) is arranged on the top of the test bench (1), a first slider (3) is arranged inside the first electric slide rail (2), a moving plate (4) is fixedly connected to the top of the first slider (3), a second electric slide rail (5) is arranged on one side of the moving plate (4), a second slider (6) is arranged inside the second electric slide rail (5), a mounting plate (7) is fixedly connected to one side of the second slider (6), a groove (20) is arranged on the bottom of the mounting plate (7), and a groove (20) is arranged inside the mounting plate (7). A connecting plate (21) is fixedly connected to the top of the wall, a first servo motor (9) is fixedly connected to the top of the mounting plate (7), an output end of the first servo motor (9) passes through the mounting plate (7) and is fixedly connected to the top of the connecting plate (21) with a first gear (24), an outer wall of the first gear (24) is evenly meshed with a second gear (26), an outer wall of the second gear (26) is meshed with an inner gear ring (23), a connecting structure is provided on the outer wall of the inner gear ring (23), a medical X-ray machine (8) is provided at the bottom of the connecting structure, and an adjustment structure is provided at the bottom of the test bench (1).

6. The device for establishing an animal model of intervertebral disc degeneration according to claim 5, characterized in that: The connection structure comprises a first annular connection plate (22), the inner wall of the first annular connection plate (22) being fixedly connected to the circumferential outer wall of the inner gear ring (23), the outer wall of the first annular connection plate (22) being slidably connected to the inside of the groove (20), the bottom of the first annular connection plate (22) being fixedly connected to a second annular connection plate (25), the bottom of the second annular connection plate (25) being fixedly connected to the top of a medical X-ray machine (8), and the medical X-ray machine (8) being located below the mounting plate (7).

7. The device for establishing an animal model of intervertebral disc degeneration according to claim 5, characterized in that: The middle portion of the second gear (26) is rotatably connected to the bottom of the connecting plate (21) via a rotating shaft, and the inner gear ring (23) is located inside the groove (20).

8. The device for establishing an animal model of intervertebral disc degeneration according to claim 5, characterized in that: The adjustment structure comprises a base (13), the top of the base (13) is symmetrically fixedly connected to a mounting block (11), the top of the mounting block (11) is provided with a slide groove (19), one side of the mounting block (11) is fixedly connected to a second servo motor (12), an output end of the second servo motor (12) passes through one side of the mounting block (11) and is fixedly connected to a bidirectional lead screw (15), an outer wall of the bidirectional lead screw (15) is symmetrically threadedly connected to a third slider (16), and the outer wall of the third slider (16) is slidably connected to the inside of the slide groove (19).

9. The device for establishing an animal model of intervertebral disc degeneration according to claim 8, characterized in that: The top of the third sliding block (16) is symmetrically fixedly connected to a first lug (17); a telescopic frame (10) is provided on a side opposite to the first lug (17); a second lug (18) is symmetrically provided on the top of the telescopic frame (10); the top of the second lug (18) is fixedly connected to a connecting block (14); and the top of the connecting block (14) is fixedly connected to the bottom of the test bench (1).

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