Composition for repairing skin barrier and resisting aging as well as preparation method and application of composition

Abstract

The invention relates to the technical field of functional skin care products, and provides a composition for skin barrier repair and aging resistance as well as a preparation method and application of the composition, and the composition comprises the following components in parts by mass: 0.052-0.8 part of a polypeptide composition, 0.5-12 parts of fibronectin, 0.05-1.2 parts of beta-nicotinamide mononucleotide, 6-14 parts of a probiotic compound factor and 0.01-1.2 parts of sodium hyaluronate. The invention further provides a preparation method of the composition and application of the composition in preparation of skin barrier repairing and anti-aging skin care products. Skin barrier repair is achieved from multiple dimensions such as a microorganism barrier, a physical barrier, a chemical barrier, an immune barrier and a pigment barrier, and meanwhile the anti-aging effect is achieved by activating cell energy, resisting inflammation and oxidation and improving the skin state.

Description

Technical Field

[0001] The present invention relates to the technical field of functional skin care products, and in particular to a composition for skin barrier repair and anti-aging, and a preparation method and application thereof. Background Art

[0002] A complete skin barrier includes multiple aspects, including microbial barrier, physical barrier, chemical barrier, immune barrier and pigment barrier. A healthy skin barrier, like a city wall, has a complete structure, including sebum membrane and stratum corneum. A complete sebum membrane coverage and a healthy stratum corneum are the best guardians of the skin.

[0003] However, excessive cleansing, lack of attention to moisturizing, frequent use of periodic skin care products, and inadequate sun protection can damage the skin barrier. Once the skin barrier is damaged, the skin becomes fragile and sensitive, and the moisturizing function decreases, which can easily lead to symptoms such as dryness, peeling, redness, burning, tightness, and itching. At the same time, after the barrier is damaged, due to the problem of lack of water and dryness, it is easy to cause pigmentation and fine lines, making the skin look "10 years older."

[0004] Skin repair is generally carried out in the following four steps: The first step is to relieve inflammation. Barrier damage is generally accompanied by sensitive skin and inflammation, red blood streaks, and crusting (similar to acne). If there are redness and itching symptoms, anti-inflammatory measures must be taken first. Only after the inflammation is relieved can the repair be continued, otherwise the skin will not be absorbed; the second step is to repair the stratum corneum. The main repair ingredients used for repair should be ingredients that are close to the human body's sebum membrane. While using the repair product, stop using all exfoliating and strong cleaning methods to repair; the third step is to replenish moisturizing factors. Repair is to build up the skin's sebum layer, and locking in moisture is to replenish the moisture in the sebum layer, increase the moisture of the outermost layer of the skin, and prevent excessive loss of moisture inside the skin, resulting in oily outside and dry inside; the fourth step is to do a good job of sun protection. The skin barrier is more susceptible to sunburn when it is damaged.

[0005] Therefore, at this stage, there is an urgent need to develop a skin care product that can repair the damaged skin barrier from multiple aspects and at the same time has an anti-aging effect. Summary of the invention

[0006] In order to solve the above problems, the present invention provides a composition for skin barrier repair and anti-aging, and its preparation method and application. The composition prepared in the present invention is composed of a polypeptide composition, fibronectin, β-nicotinamide mononucleotide, a probiotic complex factor, and sodium hyaluronate. The composition has the functions of anti-inflammatory, barrier repair, moisturizing, anti-oxidation, and anti-aging. It repairs the damaged skin barrier layer by layer, effectively improves skin hydration, increases skin hyaluronic acid content, improves photoaging, helps reverse cell glycation, and reduces the area of ​​fine lines.

[0007] To achieve the above-mentioned purpose, the present invention provides a composition for quickly removing dark circles and long-term anti-aging. The composition comprises, by weight, 0.052-0.8 parts of a polypeptide composition, 0.5-12 parts of fibronectin, 0.05-1.2 parts of β-nicotinamide mononucleotide, 6-14 parts of a probiotic complex factor, and 0.01-1.2 parts of sodium hyaluronate.

[0008] Preferably, the composition comprises, by weight, 0.12-0.66 parts of the polypeptide composition, 1-10 parts of fibronectin, 0.1-1 parts of β-nicotinamide mononucleotide, 8-12 parts of the probiotic complex factor, and 0.05-1 parts of sodium hyaluronate.

[0009] Preferably, the composition comprises, by weight, 0.5 parts of a polypeptide composition, 2.5 parts of fibronectin, 0.5 parts of β-nicotinamide mononucleotide, 10 parts of a probiotic complex factor, and 0.9 parts of sodium hyaluronate.

[0010] Optionally, the polypeptide composition comprises, by weight, 0.001 to 0.1 parts of acetyl dipeptide-1 cetyl ester, 0.001 to 0.1 parts of palmitoyl pentapeptide-4, and 0.05 to 0.6 parts of carnosine.

[0011] Preferably, the polypeptide composition comprises, by weight, 0.01 to 0.08 parts of acetyl dipeptide-1 cetyl ester, 0.01 to 0.08 parts of palmitoyl pentapeptide-4, and 0.1 to 0.5 parts of carnosine;

[0012] Preferably, the polypeptide composition comprises, by weight, 0.05 parts of acetyl dipeptide-1 cetyl ester, 0.05 parts of palmitoyl pentapeptide-4, and 0.4 parts of carnosine.

[0013] Optionally, the probiotic compound factor is prepared by compounding Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum and Lactobacillus acidophilus in a weight ratio of (1-4): (1-2): (1-2): (1-2);

[0014] Preferably, the probiotic complex factor is prepared by compounding Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum and Lactobacillus acidophilus in a weight ratio of 2:1:1:1.

[0015] Optionally, the sodium hyaluronate is any one of macromolecular sodium hyaluronate, medium molecular sodium hyaluronate, and small molecular sodium hyaluronate;

[0016] Preferably, the sodium hyaluronate is small molecule sodium hyaluronate, and its molecular weight ranges from 10K to 1000KDa.

[0017] The present invention also provides a method for preparing the composition as described above, comprising the following steps:

[0018] S1. mixing Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum and Lactobacillus acidophilus to prepare a probiotic compound factor;

[0019] S2. mixing acetyl dipeptide-1 cetyl ester, palmitoyl pentapeptide-4, carnosine, water, and butanediol to obtain a polypeptide composition;

[0020] S3. stirring fibronectin, β-nicotinamide mononucleotide, sodium hyaluronate and water to obtain an aqueous phase;

[0021] S4. Stir the probiotic complex factor, the polypeptide composition and the aqueous phase evenly to obtain a composition.

[0022] Optionally, in the preparation step, the composition is adjusted to pH 6.0 using a pH adjuster, and mixed in a 40° C. water bath for 30 minutes to obtain the composition.

[0023] The present invention also provides the use of any of the above-mentioned compositions, and / or a composition obtained by the preparation method of any of the above-mentioned compositions in cosmetics, health products, and medicines, wherein the application includes the composition being made into an emulsion, solution, paste, gel, or spray formulation.

[0024] The synergistic effect mechanism between the components in this application:

[0025] The polypeptide composition used in the present application is composed of acetyl dipeptide-1 cetyl ester, palmitoyl pentapeptide-4, and carnosine, wherein acetyl dipeptide-1 cetyl ester has α-MSH-like activity, can reduce the occurrence of inflammatory response by regulating NF-κB signal, and is currently widely used in sensitive skin cosmetics, mainly by reducing skin inflammation and improving skin barrier; palmitoyl pentapeptide-4, as a messenger peptide, can maintain skin structure stability, increase skin thickness, prevent wrinkles, delay cell aging, and increase skin water content and water lock by stimulating the synthesis of substances such as collagen and sucrose amine; carnosine can effectively remove reactive oxygen free radicals (ROS) and α-β unsaturated aldehydes formed by excessive oxidation of fatty acids in cell membranes during oxidative stress, effectively resist oxidation and repair skin damage, and also has anti-aging effects.

[0026] The fibronectin used in this application is a major non-collagenous glycoprotein that is widely present in the extracellular matrix. It can promote the growth, differentiation and migration of skin cells, help the skin to regenerate in an orderly manner, enhance the expression of barrier proteins, repair the skin barrier, improve the skin's self-protection ability, and can also stimulate its own cells to produce a variety of moisturizing factors (such as hyaluronic acid, ceramide), thereby mobilizing its own moisture from the inside out to moisturize the skin.

[0027] The β-nicotinamide mononucleotide used in this application can increase the energy level in cells and promote cell metabolism and function; this is crucial for skin health, because the increase in cellular energy can help the skin eliminate waste and toxins, keep it clean and healthy, thereby achieving the effect of skin care, making the whole person look younger and more radiant.

[0028] The probiotic complex factor used in the present application contains a large number of beneficial microorganisms to maintain the normal structure of the skin, resist the invasion of foreign pathogens, promote the skin's immune response and wound regeneration and repair, and mainly help the skin resist the stimulation and damage of external pollution such as UV and microorganisms, and build a solid defense tower; among them, Lactobacillus plantarum can inhibit the expression of MMP-1, while promoting the synthesis of collagen and preventing its decomposition, reducing the generation of ROS caused by ultraviolet radiation; Lactobacillus rhamnosus can effectively slow down the excessive production of reactive oxygen induced by UVB, reduce the decomposition of collagen and premature aging process, and enhance the expression of antioxidant genes; Lactobacillus acidophilus can reduce the mRNA expression of genes related to melanin production, such as tyrosinase, TYRP-1 and TYRP-2, and reduce UV-induced melanin deposition; Lactobacillus paracasei can reduce UVB-related oxidative damage and reduce UVB-induced photoaging process.

[0029] The small molecule sodium hyaluronate used in this application can effectively penetrate into the skin base, intensively replenish moisture, improve the skin's hydration ability, and at the same time has the function of scavenging free radicals and preventing skin aging; small molecule sodium hyaluronate can also affect collagen circulation, effectively promote collagen metabolism, and promote the production of type I procollagen, making the skin firm, moisturized, delicate and resilient.

[0030] In summary, the present invention has significant antioxidant effects through active substances such as acetyl dipeptide-1 cetyl ester, carnosine, β-nicotinamide mononucleotide, etc., which can remove free radicals, reduce oxidative damage, inhibit the production of inflammatory factors, and relieve skin inflammation. The soothing and anti-allergic effect of acetyl dipeptide-1 cetyl ester can enhance the tolerance of the skin and reduce the damage to the skin caused by external stimuli. The DNA repair effect of β-nicotinamide mononucleotide can help maintain the healthy state of skin cells and promote cell repair and regeneration. Palmitoyl pentapeptide-4 and fibronectin act on dermal fibroblasts to effectively promote the synthesis of macromolecular substances such as type I, III, IV collagen, fibronectin and glycosaminoglycans, repair and rebuild the extracellular matrix and the epidermis-dermis junction, and repair the stratum corneum. Small molecule sodium hyaluronate and fibronectin adsorb and retain water molecules to maintain the moisture of the skin, making the skin softer and smoother. The probiotic compound factor is used to improve the microecology on the surface of the skin. The present invention achieves skin barrier repair from multiple dimensions such as microbial barrier, physical barrier, chemical barrier, immune barrier and pigment barrier, and at the same time improves skin condition and achieves anti-aging effect by activating cell energy, anti-inflammation and anti-oxidation.

[0031] The beneficial effects of the present invention are embodied in:

[0032] (1) The composition of the present invention has an improving effect on the microecology of the skin surface. The probiotic compound factor occupies a dominant position in the microecology of the skin surface, reducing the hidden dangers caused by the imbalance of the skin surface flora;

[0033] (2) The composition of the present invention can repair the skin barrier, taking into account the ways in which the skin barrier is damaged, and repairs the skin from multiple perspectives, including microbial barrier, physical barrier, chemical barrier, immune barrier and pigment barrier;

[0034] (3) The raw materials provided by the present invention are environmentally friendly, healthy, non-toxic, non-addictive, and can be used for a long time. At the same time, they can activate cell energy, resist inflammation, and resist oxidation, improve skin condition, and achieve anti-aging effects. They can be used alone in the form of emulsions, solutions, pastes, gels, or sprays, or used in combination with other skin care products. DETAILED DESCRIPTION

[0035] In order to more clearly illustrate the overall concept of the present invention, a detailed description is given below by way of examples.

[0036] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0037] Unless otherwise specified, the raw materials, reagents or devices used in the following examples can be obtained from conventional commercial sources or can be obtained by existing known methods; unless otherwise specified, the methods used in the examples of the present invention are methods known to those skilled in the art.

[0038] Hereinafter, a composition for skin barrier repair and anti-aging, and a preparation method and application thereof in the present invention will be described in detail in combination with exemplary embodiments.

[0039] Example 1 Preparation of the composition

[0040] The preparation method of composition 1# comprises the following steps:

[0041] 1. Mix 32g of Lactobacillus rhamnosus, 16g of Lactobacillus paracasei, 16g of Lactobacillus plantarum and 16g of Lactobacillus acidophilus to prepare a probiotic compound factor;

[0042] 2. Mix 0.1 g of acetyl dipeptide-1 cetyl ester, 0.1 g of palmitoyl pentapeptide-4, 1 g of carnosine, 300 g of water, and 30 g of butanediol to obtain a polypeptide composition;

[0043] 3. Stir 10 g of fibronectin, 1 g of β-nicotinamide mononucleotide, 0.5 g of small molecule sodium hyaluronate and 360 g of water to obtain an aqueous phase;

[0044] 4. Evenly stir the probiotic complex factor, polypeptide composition and aqueous phase, add triethanolamine to adjust the pH value to 6.0, place in a water bath at 40°C for 30 minutes and stir to mix, to obtain composition 1#.

[0045] The composition with skin barrier repair and anti-aging effects prepared above is recorded as composition 1#; according to the preparation method of composition 1#, the component dosage ratio is adjusted to prepare compositions 1#-14# and compositions D1#-D4#. The specific formulas are shown in Table 1. Compositions 1#-14# are example experimental groups, and compositions D1#-D4# are comparative experimental groups.

[0046] Table 1. Composition formula

[0047]

[0048]

[0049] Example 2

[0050] The composition prepared by the method of Reference Example 1 is different in that Example 2 is set according to the parameter conditions of Composition 2# in Table 1, and other conditions are the same as Example 1, to prepare Composition 2#.

[0051] Example 3

[0052] The composition prepared by the method of Reference Example 1 is different in that Example 3 is set according to the parameter conditions of Composition 3# in Table 1, and other conditions are the same as Example 1, to prepare Composition 3#.

[0053] Example 4

[0054] The composition prepared by the method of Reference Example 1 is different in that Example 4 is set according to the parameter conditions of Composition 4# in Table 1, and other conditions are the same as Example 1, to prepare Composition 4#.

[0055] Example 5

[0056] The composition prepared by the method of Reference Example 1 is different in that Example 5 is set according to the parameter conditions of Composition 5# in Table 1, and other conditions are the same as Example 1, to prepare Composition 5#.

[0057] Example 6

[0058] The composition prepared by the method of reference Example 1 is different in that Example 6 is set according to the parameter conditions of composition 6# in Table 1, and acetyl dipeptide-1 cetyl ester is replaced by acetyl heptapeptide-4. Other conditions are the same as in Example 1, to prepare composition 6#.

[0059] Example 7

[0060] The composition prepared by the method of reference Example 1 is different in that Example 7 is set according to the parameter conditions of composition 7# in Table 1, and palmitoyl tripeptide-5 replaces palmitoyl pentapeptide-4. The other conditions are the same as in Example 1, and composition 7# is prepared.

[0061] Example 8

[0062] The composition prepared by the method of Reference Example 1 is different in that Example 8 is set according to the parameter conditions of Composition 8# in Table 1, and glutathione is used instead of carnosine. Other conditions are the same as those of Example 1, to prepare Composition 8#.

[0063] Example 9

[0064] The composition prepared by the method of reference Example 1 is different in that Example 9 is set according to the parameter conditions of composition 9# in Table 1, and oligopeptide-1, oligopeptide-3, and glutathione are used to replace the polypeptide complex. The other conditions are the same as those of Example 1 to prepare composition 9#.

[0065] Example 10

[0066] The composition prepared by the method of Reference Example 1 is different in that Example 10 is set according to the parameter conditions of composition 10# in Table 1, and Bifidobacterium longum and Lactobacillus reuteri replace Lactobacillus rhamnosus and Lactobacillus paracasei. Other conditions are the same as those of Example 1, to prepare composition 10#.

[0067] Embodiment 11

[0068] The composition prepared by the method of Reference Example 1 is different in that Example 11 is set according to the parameter conditions of composition 11# in Table 1, and Bifidobacterium animalis and Lactobacillus helveticus replace Lactobacillus paracasei and Lactobacillus plantarum. Other conditions are the same as those of Example 1, to prepare composition 11#.

[0069] Example 12

[0070] The composition prepared by the method of Reference Example 1 is different in that Example 12 is set according to the parameter conditions of composition 12# in Table 1, and Bifidobacterium and Enterococcus replace Lactobacillus plantarum and Lactobacillus acidophilus. Other conditions are the same as those of Example 1, to prepare composition 12#.

[0071] Embodiment 13

[0072] The composition prepared by the method of reference Example 1 is different in that Example 13 is set according to the parameter conditions of composition 13# in Table 1, and the mass ratio between the components of the polypeptide composition is adjusted. The other conditions are the same as those of Example 1, and composition 13# is prepared.

[0073] Embodiment 14

[0074] The composition prepared by the method of reference Example 1 is different in that Example 14 is set according to the parameter conditions of composition 14# in Table 1, and the mass ratio of Lactobacillus rhamnosus: Lactobacillus paracasei: Lactobacillus plantarum: Lactobacillus acidophilus in the probiotic composite factor is adjusted to 1:3:3:1, and the other conditions are the same as those of Example 1, to prepare composition 14#.

[0075] Comparative Example D1

[0076] The composition prepared by the method of Reference Example 1 is different in that the comparative example D1 is set according to the parameter conditions of the composition D1# in Table 1, and no polypeptide complex is added. The other conditions are the same as those of Example 1, and the composition D1# is prepared.

[0077] Comparative Example D2

[0078] The composition prepared by the method of Reference Example 1 is different in that the comparative example D2 is set according to the parameter conditions of the composition D2# in Table 1, and no probiotic compound factor is added. The other conditions are the same as those of Example 1, and the composition D2# is prepared.

[0079] Comparative Example D3

[0080] The composition prepared by the method of Reference Example 1 is different in that the comparative example D3 is set according to the parameter conditions of the composition D3# in Table 1, and elastin is used instead of fibronectin. The other conditions are the same as those of Example 1, to prepare the composition D3#.

[0081] Comparative Example D4

[0082] The composition prepared by the method of Reference Example 1 is different in that the comparative example D4 is set according to the parameter conditions of the composition D4# in Table 1, and the β-nicotinamide mononucleotide is replaced by polydeoxyribonucleotide. The other conditions are the same as those in Example 1, and the composition D4# is prepared.

[0083] Experimental example

[0084] The above-mentioned examples and comparative examples were tested for soothing and anti-inflammatory activity, moisturizing effect, antioxidant performance and anti-aging performance, respectively, and the test methods are as follows:

[0085] 1. Soothing and anti-inflammatory activity test: For the anti-inflammatory test, 19 culture media were prepared, numbered 1-19# in sequence, and HaCaT (human keratinocytes) were cultured in the above-mentioned volumetric flasks with RPMI-1640 medium containing 10% fetal bovine serum, 100U / ml penicillin, and 100μg / ml streptomycin at 37°C, 5% CO2, and 98% saturated humidity; then, compositions 1-14# (final concentration 0.2mg / mL) were added to the culture media numbered 1-14#, respectively, and compositions D1-D4# (final concentration 0.2mg / mL) were added to the culture media numbered 15-18#, respectively, and 19# was a blank control group. Cells in logarithmic growth phase were selected and inoculated into 96-well culture plates. After culturing for 24 hours, the culture medium was replaced with RPMI-1640 culture medium containing composition 1-14# and composition D1-D4#, respectively. After incubation for 12 hours, the cells were irradiated with a dose of 30mJ / cm2 of UVB at a distance of 15cm. After irradiation, the cell culture supernatant was collected and the concentration of IL-1α in the supernatant was detected using a human ELISA kit. Each experiment was repeated three times, and the average value was taken as the final result. The results are shown in Table 2.

[0086] The soothing test is expressed by the hyaluronidase inhibition rate: prepare 19 transparent volumetric flasks, numbered 1-18# in sequence, and add 0.1mL of 2.5mmol / LCaCl2 and 0.5mL of hyaluronidase (600U / mL) to the above volumetric flasks in sequence, and keep warm at 37℃ for 20min; then add 1mL of composition 1-14# test solution to 1-14# volumetric flasks respectively, and add 1mL of composition D1-D4# test solution to 15-18# volumetric flasks respectively, and 19# is the control group. Keep warm at 37℃ for 20min; add 2mL of 0.4mg / mL sodium hyaluronate, and keep warm at 37℃ for 40min; add 1mL of acetylacetone solution, place in a boiling water bath and heat for 15min, and immediately cool with ice water for 5min; finally add 1.0mL of Ehrlich reagent, place at room temperature for 60min to develop color, and measure the absorbance of each group of test solution at 530nm.

[0087]

[0088] Hyaluronidase inhibition rate (%) = [(VA-VB)-(VC-VD)] / (VA-VB) × 100%;

[0089] Where VA represents the absorbance of the control solution (using acetate buffer instead of sample solution); VB represents the absorbance of the control blank solution (using acetate buffer instead of sample solution and enzyme solution); VC represents the absorbance of the test solution; VD represents the absorbance of the sample blank solution (using acetate buffer instead of enzyme solution).

[0090] Table 2: Soothing and anti-inflammatory activity test results

[0091] Group IL-1α inhibition rate % Hyaluronidase inhibition rate % Composition 1# 76.8 86.7 Composition 2# 79.3 89.1 Composition 3# 76.5 86.1 Composition 4# 74.7 84.3 Composition 5# 75.1 83.8 Composition 6# 65.2 713 Composition 7# 66.3 72.7 Composition 8# 64.5 70.6 Composition 9# 60.3 67.6 Composition 10# 67.7 70.2 Composition 11# 68.2 71.1 Composition 12# 68.4 72.6 Composition 13# 67.6 72.5 Composition 14# 69.2 73.3 Composition D1# 43.5 54.7 Composition D2# 55.8 58.4 Composition D3# 68.2 69.6 Composition D4# 67.6 68.5

[0092] Analysis of test results: From the experimental results in Table 2, it can be seen that the components of compositions 1-5# in the present invention are reasonably matched and balanced, have a high elimination rate of inflammatory factors, can quickly penetrate the skin to exert soothing and anti-inflammatory effects, and compositions 1-3# have the best improvement effect, especially composition 2# has the best effect in eliminating inflammatory factors and soothing and anti-inflammatory effects. This shows that the polypeptide composition, fibronectin, β-nicotinamide mononucleotide, probiotic compound factor, and sodium hyaluronate in the present invention can synergize with each other to achieve better anti-inflammatory effects.

[0093] 2. Moisturizing effect test: 54 female subjects aged 18 to 22 were selected and randomly divided into Example 1-14 group and Comparative Example 1-4 group, with 3 subjects randomly assigned to each group. In an environment with a temperature of 25±2°C and a relative humidity of 50±2, the subjects' cheeks were wiped clean and sat for half an hour. The initial skin moisture value of the cheek was measured using a capacitive skin moisture meter, and then the initial skin moisture value of the cheek was measured at 2g / cm 2The compositions were applied in different amounts (the concentration of the experimental compositions was 8%), wherein the Example 1-14 groups were applied with compositions 1-14#, and the Comparative Example 1-4 groups were applied with compositions D1-D4#, and the average growth rate of the skin moisture value relative to the initial value of the skin moisture value was tested for each group after 2 hours and 8 hours. The specific results are shown in Table 3:

[0094]

[0095] Table 3. Moisturizing effect test results

[0096]

[0097] Analysis of test results: From the moisturizing effect verification results in Table 3, it can be seen that the average growth rate of skin moisture value of compositions 1-5# in the present invention after 2 hours and 8 hours is greater than that of the other compositions, indicating that the water absorption and moisturizing effect of the composition components prepared in this application is more lasting, and the improvement effect of compositions 1-3# is more obvious, especially composition 2# is more lasting in terms of water absorption and moisturizing effect. It shows that the polypeptide composition, fibronectin, β-nicotinamide mononucleotide, probiotic complex factor, and sodium hyaluronate in the present invention can synergize with each other, have just the right long-term water absorption and moisturizing effect, and are suitable for long-term hydration and moisturizing.

[0098] 3. Antioxidant performance test: Referring to T / SHRH 032-2020 "Cosmetic Firming and Anti-wrinkle-In vitro Keratinocyte Reactive Oxygen Species (ROS) Inhibition Test Method", compositions 1-14# and compositions D1-D4# were subjected to cell active oxygen scavenging tests. The test concentration of each composition was 10%. The test results are shown in Table 4.

[0099]

[0100] Table 4. Antioxidant performance test results

[0101] Group ROS inhibition rate (unit %) Blank Group 0 Composition 1# 61.4 Composition 2# 63.7 Composition 3# 60.8 Composition 4# 59.7 Composition 5# 60.6 Composition 6# 53.6 Composition 7# 52.1 Composition 8# 52.3 Composition 9# 46.3 Composition 10# 54.7 Composition 11# 53.8 Composition 12# 54.2 Composition 13# 55.5 Composition 14# 56.1 Composition D1# 41.7 Composition D2# 48.9 Composition D3# 53.6 Composition D4# 55.1

[0102] Analysis of test results: From the test results in Table 4, it can be seen that the compositions 1-5# in the present invention can significantly inhibit ROS, and the inhibitory effect of compositions 1-3# on ROS is more obvious, especially the inhibitory effect of composition 1# is the best. This indicates that the components of the polypeptide composition, fibronectin, β-nicotinamide mononucleotide, probiotic complex factor, and sodium hyaluronate in the present application have a certain synergistic effect, which has an improvement effect on the removal of ROS by keratinocytes, and the composition in the present application has good antioxidant properties.

[0103] 4. Anti-aging performance test: 180 female volunteers aged 30-55 with wrinkles and healthy skin were selected and randomly divided into 18 groups, with 10 in each group. In an environment with a temperature of 25±2℃ and a relative humidity of 50±2, wipe the cheeks of the subjects and sit for half an hour. Then, the composition 1-14# was used for the members of groups 1-14, and the composition D1-D4# was used for the members of groups 15-18. The test concentration of each composition was 10%, and it was used once in the morning and evening every day for one cycle, and one cycle was 90 days. The skin wrinkle state was measured before and after the cycle of use, and recorded as the initial wrinkle measurement value and the 90d wrinkle measurement value respectively. When measuring the wrinkle state, the skin fast three-dimensional imaging system PRIMOS was used to detect five places in total, including the left and right corners of the eyes, the middle of the forehead, and the cheeks on both sides. The measurement value was the average of the five detections, and the average of the test data of each group was taken. The test data was rounded to two decimal places. The lower the measurement value, the fewer skin wrinkles.

[0104] Table 5. Anti-aging performance test results

[0105] Group Initial wrinkle measurements 90d wrinkle measurement Composition 1# 38.24 31.27 Composition 2# 38.56 30.01 Composition 3# 37.99 31.38 Composition 4# 37.73 31.21 Composition 5# 38.59 31.57 Composition 6# 38.55 33.17 Composition 7# 38.45 33.25 Composition 8# 38.42 33.06 Composition 9# 37.75 34.68 Composition 10# 37.94 32.63 Composition 11# 37.97 32.28 Composition 12# 38.07 32.72 Composition 13# 38.19 32.87 Composition 14# 38.52 32.43 Composition D1# 38.85 36.13 Composition D2# 38.27 34.59 Composition D3# 38.65 33.21 Composition D4# 38.49 33.17

[0106] Analysis of test results: It can be seen from Table 5 that the composition prepared by the present invention has excellent anti-aging properties. In detail, it can be seen from Examples 1-3 that the present invention uses active substances such as acetyl dipeptide-1 cetyl ester, carnosine, β-nicotinamide mononucleotide to inhibit the production of inflammatory factors and relieve skin inflammation; at the same time, palmitoyl pentapeptide-4 and fibronectin act on dermal fibroblasts to promote the synthesis of macromolecular substances such as type I, III, IV collagen, fibronectin and glycosaminoglycans, repair and rebuild the extracellular matrix and the epidermis-dermis junction, and repair the stratum corneum; small molecule sodium hyaluronate and fibronectin synergistically adsorb and retain water molecules, maintain the moisture of the skin, and make the skin softer and smoother; probiotic compound factors are used to improve the microecology of the skin surface. The synergistic effect of each component effectively increases the anti-aging performance of the composition.

[0107] Combined with the results of the above experiments, it can be seen that the composition of the present invention can achieve excellent skin barrier repair effect and better anti-aging performance, mainly for the following reasons: inhibiting the production of inflammatory factors through active substances such as acetyl dipeptide-1 cetyl ester, carnosine, β-nicotinamide mononucleotide, and relieving skin inflammation; through palmitoyl pentapeptide-4 and fibronectin acting on dermal fibroblasts, promoting the synthesis of macromolecular substances such as type I, III, IV collagen, fibronectin and glycosaminoglycans, repairing and rebuilding the extracellular matrix and the epidermis-dermis junction, and repairing the stratum corneum; through small molecule sodium hyaluronate and fibronectin adsorbing and retaining water molecules, maintaining the moisture of the skin, making the skin softer and smoother; through probiotic composite factors, the improvement of the microecology of the skin surface is achieved. Accordingly, the present invention realizes skin barrier repair from multiple dimensions such as microbial barrier, physical barrier, chemical barrier, immune barrier and pigment barrier, and at the same time, by activating cell energy, anti-inflammatory and anti-oxidation, improves skin condition and achieves anti-aging effect.

[0108] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A composition for skin barrier repair and anti-aging, characterized in that: Calculated by weight, the composition includes 0.052-0.8 parts of a polypeptide composition, 0.5-12 parts of fibronectin, 0.05-1.2 parts of β-nicotinamide mononucleotide, 6-14 parts of a probiotic complex factor, and 0.01-1.2 parts of sodium hyaluronate.

2. The composition according to claim 1, characterized in that Calculated by weight, the composition includes 0.12-0.66 parts of a polypeptide composition, 1-10 parts of fibronectin, 0.1-1 parts of β-nicotinamide mononucleotide, 8-12 parts of a probiotic complex factor, and 0.05-1 parts of sodium hyaluronate.

3. The composition according to claim 1, characterized in that Calculated by weight, the composition includes 0.5 parts of a polypeptide composition, 2.5 parts of fibronectin, 0.5 parts of β-nicotinamide mononucleotide, 10 parts of a probiotic complex factor, and 0.9 parts of sodium hyaluronate.

4. The composition according to claim 1, characterized in that Calculated by weight, the polypeptide composition includes 0.001-0.1 parts of acetyl dipeptide-1 cetyl ester, 0.001-0.1 parts of palmitoyl pentapeptide-4, and 0.05-0.6 parts of carnosine.

5. The composition according to claim 1, characterized in that Calculated by weight, the polypeptide composition includes 0.01 to 0.08 parts of acetyl dipeptide-1 cetyl ester, 0.01 to 0.08 parts of palmitoyl pentapeptide-4, and 0.1 to 0.5 parts of carnosine; Preferably, the polypeptide composition comprises, by weight, 0.05 parts of acetyl dipeptide-1 cetyl ester, 0.05 parts of palmitoyl pentapeptide-4, and 0.4 parts of carnosine.

6. The composition according to claim 1, characterized in that The probiotic compound factor is prepared by compounding Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum and Lactobacillus acidophilus in a weight ratio of (1-4): (1-2): (1-2): (1-2); Preferably, the probiotic complex factor is prepared by compounding Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum and Lactobacillus acidophilus in a weight ratio of 2:1:1:

1.

7. The composition according to claim 1, characterized in that The sodium hyaluronate is any one of macromolecular sodium hyaluronate, medium molecular sodium hyaluronate and small molecular sodium hyaluronate; Preferably, the sodium hyaluronate is small molecule sodium hyaluronate, and its molecular weight ranges from 10K to 1000KDa.

8. A method for preparing the composition for skin barrier repair and anti-aging according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. mixing Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus plantarum and Lactobacillus acidophilus to prepare a probiotic compound factor; S2. mixing acetyl dipeptide-1 cetyl ester, palmitoyl pentapeptide-4, carnosine, water, and butanediol to obtain a polypeptide composition; S3. stirring fibronectin, β-nicotinamide mononucleotide, sodium hyaluronate and water to obtain an aqueous phase; S4. Stir the probiotic complex factor, the polypeptide composition and the aqueous phase evenly to obtain a composition.

9. The method for preparing the composition according to claim 8, characterized in that: In the preparation step, the pH value of the composition is adjusted to 6.0 by using a pH adjuster, and the mixture is mixed in a water bath at 40° C. for 30 minutes to obtain the composition.

10. Use of the composition according to any one of claims 1 to 7, and / or the composition obtained by the method for preparing the composition according to any one of claims 8 to 9, in cosmetics, health products, and medicines, wherein the application includes the composition being made into an emulsion, solution, paste, gel, or spray formulation.

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