Single cell culture device and method thereof

Abstract

The invention relates to the technical field of single cell culture, in particular to a single cell culture device and a method thereof.The single cell culture device comprises an oscillation barrel and a carrying platform, a shell is fixedly installed on the surface of the upper end of the carrying platform, two sliding rods are inserted into the outer side wall of the shell, and one ends of the two sliding rods are fixedly connected with the oscillation barrel; buffer springs are arranged outside the two sliding rods and located between the outer wall of the oscillation barrel and the inner wall of the shell in a sleeving mode, a second circular plate is fixedly installed at the position, close to the bottom, inside the oscillation barrel, a placing table is movably installed above the second circular plate, and an auxiliary mechanism is arranged between the bottom face of the placing table and the surface of the upper end of the second circular plate; according to the invention, the test tube can be vertically vibrated while being transversely vibrated, so that cells and a culture solution are fully oscillated and fused, and single cells transferred to the culture dish and the culture solution can be automatically oscillated and fused again, so that the single cells and the culture solution can be fully and uniformly fused, and the culture effect and efficiency of the single cells are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of single cell culture, in particular to a single cell culture device and a method thereof. Background Art

[0002] Single-cell culture refers to the technology of isolating single cells from plant organs, callus tissue or suspension cultures and growing and developing them in vitro under sterile conditions. People had the idea and practice of isolating and culturing single plant cells relatively early, but successful single-cell culture was only achieved with the development of more effective culture media and the establishment of specialized techniques for isolating single cells from callus tissue suspension cultures.

[0003] In the prior art, there is a cell culture device suitable for single cells proposed in patent application number "CN201911118766.9". When the culture dish accidentally falls from the test bench, the bottom end of the culture dish will fall vertically due to the weight of the circular metal weight block, which can effectively prevent the top end from falling vertically, so as to prevent the culture fluid in the culture dish from contacting the dish cover and flowing out from the air vent. The dish cover and the culture dish are connected by a rubber ball A and an outer annular arc-shaped limit groove, so as to ensure that the dish cover will not be separated from the culture dish when the culture dish falls.

[0004] However, in the above patent application, when culturing single cells, it is usually necessary to oscillate the single cells together with the culture medium so that the cells can be evenly fused with the culture medium to improve the culture efficiency. However, nowadays, when culturing single cells, it is generally only done by manual oscillation, and the adequacy of the oscillation cannot be guaranteed. When using an oscillating device, it only oscillates in a single direction, resulting in a poor oscillation fusion effect. Summary of the invention

[0005] The object of the present invention is to provide a single cell culture device and method thereof to solve the problems raised in the above background technology.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A single cell culture device comprises an oscillating barrel and a carrier, the upper surface of the carrier is fixedly mounted with an outer shell, the outer wall of the outer shell is inserted with two sliding rods, and one end of the two sliding rods are fixedly connected to the oscillating barrel, and a buffer spring is sleeved on the outside of the two sliding rods and located between the inner wall of the outer shell of the oscillating barrel outer wall, a circular plate 2 is fixedly mounted near the bottom position inside the oscillating barrel, a placing table is movably mounted above the circular plate 2, an auxiliary mechanism is arranged between the bottom surface of the placing table and the upper end surface of the circular plate 2, the bottom surface of the placing table is fixedly connected with an annular plate, the upper surface of the annular plate is provided with a plurality of circular slots, and test tubes are inserted in the plurality of circular slots, a circular plate 1 is fixedly mounted on the upper surface of the carrier, a plurality of culture dishes are fixedly mounted on the upper surface of the circular plate 1, the upper surface of the circular plate 1 is rotatably connected with a vertical shaft near the middle position, and the upper end surface of the vertical shaft is provided with a clamping mechanism.

[0008] Preferably, the bottom end of the oscillation barrel is rotatably connected to an eccentric wheel via an axle rod, a motor is installed on the bottom surface of the oscillation barrel, and the output end of the motor is connected to the eccentric wheel, two telescopic push rods are fixedly connected to the upper end surface of the circular plate 2, and the upper ends of the two telescopic push rods are fixedly connected to the placement table, and the outsides of the two telescopic push rods are both sleeved with support springs.

[0009] Preferably, the auxiliary mechanism includes a bevel gear 2 and two mounting plates, the bevel gear 2 is rotatably connected to the upper end surface of the circular plate 2 through an axle, both mounting plates are fixedly installed on the upper end surface of the circular plate 2, and the bevel gear 2 is connected to the eccentric wheel through the axle.

[0010] Preferably, the outer walls of the two mounting plates are rotatably connected to a short shaft, one end of the short shaft is fixedly connected to a bevel gear 1, and the bevel gear 1 and the bevel gear 2 are meshed with each other, and the other end of the short shaft is fixedly connected to a turntable, and the outer wall of the turntable is rotatably connected to a connecting rod 1 via an axis rod, and the end of the connecting rod 1 away from the turntable is rotatably connected to a connecting rod 2 via an axis rod, and the upper end of the connecting rod 2 is fixedly connected to the placement table.

[0011] Preferably, a plurality of placement grooves are provided on the outer side wall of the placement table, a connecting shaft is rotatably connected to the upper end surface of the placement table near the middle position, a central gear is fixedly connected to the outer wall of the connecting shaft near the bottom end position, a second motor is installed on the bottom surface of the placement table, and the output end of the second motor is connected to the connecting shaft;

[0012] The upper surface of the placing table is installed with clamps near the edge positions of several placing grooves, and one of the clamps is fixedly connected to the upper surface of the placing table, and the other clamp is movably installed on the upper surface of the placing table. One end of one of the clamps is fixedly connected to a movable plate, and the movable plate is rotatably connected to the upper surface of the placing table through an axle rod. The upper surface of the movable plate is fixedly connected to a transmission gear through an axle rod, and the transmission gear and the center gear are meshed with each other.

[0013] Preferably, the upper end of the connecting shaft is rotatably connected to a ratchet through an axle rod, a limiting groove is provided on the bottom surface of the ratchet, the outer wall of the connecting shaft is rotatably connected to a limiting block located inside the limiting groove, and the limiting block and the limiting groove are meshed with each other.

[0014] Preferably, the upper end surface of the shell is fixedly connected to a bracket, the upper end surface of the ratchet is fixedly connected to a transmission shaft, and the upper end of the transmission shaft is rotatably connected to the top position inside the bracket, the outer side wall of the shell is rotatably connected to a long shaft, a transmission belt 2 is sleeved between the long shaft and the outside of the transmission shaft, and a transmission belt 1 is sleeved between the vertical axis and the outside of the long shaft.

[0015] Preferably, two frames are fixedly connected to the inner wall of the shell and the lower part of the oscillation barrel, and slide plates are slidably connected inside the two frames, and the upper ends of the two slide plates are fixedly connected to the oscillation barrel.

[0016] Preferably, the clamping mechanism includes a connecting rod, which is fixedly connected to the upper surface of the vertical axis, one end of the connecting rod is matched with the bottom end of the slide, and movable grooves are provided on both outer walls of the slide near the bottom, and clamping blocks are inserted into the two movable grooves, and clamping grooves are provided on both inner walls of the connecting rod, and the clamping grooves are matched with the clamping blocks, and a limiting spring is installed between one end of the clamping block and the inner wall of the movable groove.

[0017] A working method of a single cell culture device, the working method of the single cell culture device specifically comprises the following steps:

[0018] Step 1: Transfer the cells to be cultured into a test tube, inject the culture medium and seal it, then place it in a placement slot on the placement table. Similarly, place multiple test tubes containing single cells and culture medium in other placement slots in sequence.

[0019] Step 2: The connecting shaft is driven to rotate by the second motor, thereby driving the central gear to rotate, and driving each transmission gear to rotate, and then driving the clamping plate to rotate until its inner wall is close to the outer wall of the test tube, clamping and fixing the test tube, and similarly clamping and fixing several other test tubes at the same time;

[0020] Step 3: The eccentric wheel is driven to rotate by the motor 1, so that the oscillation barrel generates a lateral vibration force, and each test tube is vibrated laterally. When the eccentric wheel rotates, the bevel gear 2 rotates accordingly, driving the two bevel gears 1 to rotate, so that the turntable rotates, and the connecting rod 1 and the connecting rod 2 are coordinated to make the placement table move up and down reciprocatingly, generating a vertical vibration force, and vibrating each test tube vertically. The test tube is vibrated laterally and vertically at the same time, so that the cells and the culture solution are fully vibrated and fused;

[0021] Step 4: The connecting shaft is driven to reverse by the second motor to release the clamping of the test tube, and then the single cells after each oscillation and fusion are put into each culture dish in turn, and the corresponding culture medium is added to transfer the single cells to the culture dish for culture;

[0022] Step 5: The end of the connecting rod away from the vertical axis is engaged and fixed with the bottom of the slide, and then the eccentric wheel is driven to rotate by the motor 1, so that the oscillation barrel moves back and forth left and right, and the single cells and culture fluid inside each culture dish placed on the circular plate 1 are automatically oscillated left and right, and finally left to stand to complete the culture.

[0023] Beneficial effects of the present invention:

[0024] 1. In the present invention, the motor 1 cooperates with the eccentric wheel to rotate, so that the oscillation barrel generates a lateral vibration force, and each test tube is vibrated laterally. When the eccentric wheel rotates, the bevel gear 2 rotates accordingly, driving the two bevel gears 1 to rotate, and the turntable connected with the short shaft rotates, so that one end of the connecting rod 1 rotates with the turntable and moves in a circular trajectory, and the other end of the connecting rod 1 rotates with the connecting rod 2, and can drive the connecting rod 2 to move back and forth in the vertical direction, thereby driving the placement table to move back and forth up and down, generating a vertical vibration force, and vibrating each test tube vertically. Through the above operation, the test tube can be vibrated laterally and vertically at the same time, so that the cells and the culture solution are fully oscillated and fused.

[0025] 2. The present invention rotates the clamping plate to release the clamping and fixing of the test tube, drives the ratchet wheel to rotate, and makes the connecting rod in the clamping mechanism rotate accordingly, so that the end of the connecting rod away from the vertical axis is clamped and fixed with the bottom of the slide plate, and the eccentric wheel is driven by the motor to rotate, so that the oscillation barrel reciprocates left and right, and the slide plate can be driven to reciprocate left and right along the inside of the frame, so that the single cells and the culture solution in each culture dish placed on the circular plate are automatically oscillated left and right, and the single cells and the culture solution are automatically oscillated and fused again before they are still, so that the single cells and the culture solution can be fully and evenly fused, thereby improving the culture effect and efficiency of the single cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 It is a partial structural schematic diagram of the present invention;

[0029] Figure 3 is a cross-sectional view of the oscillation barrel and the housing in the present invention;

[0030] Figure 4 The present invention Figure 3 The enlarged view of point A in the middle;

[0031] Figure 5 It is a schematic diagram of the structure of the placing table, the central gear and the clamping plate in the present invention;

[0032] Figure 6 It is a structural schematic diagram of the placement table and the telescopic push rod in the present invention;

[0033] Figure 7 It is a structural schematic diagram of the circular plate 2, the rotating disk, the connecting rod 1 and the connecting rod 2 in the present invention;

[0034] Figure 8 It is a structural schematic diagram of the connecting rod and the card slot in the present invention;

[0035] Fig. 9 It is a structural schematic diagram of the ratchet and the limiting groove in the present invention.

[0036] The reference numerals in the figures are as follows:

[0037] 1. Oscillating barrel; 2. Shell; 3. Carrier; 5. Placement table; 6. Bracket; 7. Slide rod; 8. Round plate 1; 9. Culture dish; 10. Frame; 11. Eccentric wheel; 12. Round plate 2; 13. Telescopic push rod; 14. Annular plate; 15. Connecting shaft; 16. Ratchet; 17. Placement slot; 18. Clamp; 19. Transmission gear; 20. Movable plate; 21. Mounting plate; 22. Short shaft; 23. Bevel gear 1; 24. Connecting rod 1; 25. Connecting rod 2; 26. Bevel gear 2; 27. Long shaft; 28. Vertical shaft; 29. ​​Connecting rod; 30. Limiting slot; 31. Limiting block; 32. Transmission belt 1; 33. Transmission belt 2; 34. Turntable; 35. Slide plate; 36. Block; 37. Slot; 38. Test tube; 39. Center gear; 40. Transmission shaft. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] A single cell culture device and method thereof, such as Figure 1-Figure 9 As shown, it includes an oscillation barrel 1 and a carrier 3, the upper end surface of the carrier 3 is fixedly mounted with a shell 2, the outer wall of the shell 2 is inserted with two slide bars 7, and one end of the two slide bars 7 is fixedly connected to the oscillation barrel 1, and a buffer spring is sleeved on the outside of the two slide bars 7 and located between the outer wall of the oscillation barrel 1 and the inner wall of the shell 2. A circular plate 2 12 is fixedly mounted near the bottom of the oscillation barrel 1, a placing table 5 is movably mounted above the circular plate 2 12, an auxiliary mechanism is arranged between the bottom surface of the placing table 5 and the upper end surface of the circular plate 2 12, an annular plate 14 is fixedly connected to the bottom surface of the placing table 5, a plurality of circular slots are opened on the upper end surface of the annular plate 14, and test tubes 38 are inserted in the plurality of circular slots, a circular plate 1 8 is fixedly mounted on the upper end surface of the carrier 3, a plurality of culture dishes 9 are fixedly mounted on the upper end surface of the circular plate 1 8, a vertical shaft 28 is rotatably connected to the upper end surface of the circular plate 1 8 near the middle, and a clamping mechanism is arranged on the upper end surface of the vertical shaft 28.

[0040] The bottom end of the oscillation barrel 1 is rotatably connected to an eccentric wheel 11 through an axis rod. A motor 1 is installed on the bottom surface of the oscillation barrel 1, and the output end of the motor 1 is connected to the eccentric wheel 11. Two telescopic push rods 13 are fixedly connected to the upper end surface of the circular plate 2 12, and the upper ends of the two telescopic push rods 13 are fixedly connected to the placement table 5. The two telescopic push rods 13 are both sleeved with support springs on the outside. When the platform 3 moves back and forth vertically and generates vertical vibration force, the two telescopic push rods 13 are repeatedly stretched and compressed following the placement table 5, and the support springs are repeatedly stretched and compressed accordingly, which plays a role in limiting and supporting the vertical moving position of the placement table 5, so that the placement table 5 can maintain a vertical direction and move back and forth up and down.

[0041] like Figure 7 As shown, the auxiliary mechanism includes a bevel gear 26 and two mounting plates 21. The bevel gear 26 is rotatably connected to the upper end surface of the circular plate 12 through an axle. Both mounting plates 21 are fixedly installed on the upper end surface of the circular plate 12, and the bevel gear 26 is connected to the eccentric wheel 11 through the axle.

[0042] The outer side walls of the two mounting plates 21 are rotatably connected to a short shaft 22, one end of the short shaft 22 is fixedly connected to a bevel gear 1 23, the bevel gear 1 23 and the bevel gear 2 26 are meshed with each other, the other end of the short shaft 22 is fixedly connected to a turntable 34, the outer side wall of the turntable 34 is rotatably connected to a connecting rod 1 24 via an axle rod, the end of the connecting rod 1 24 away from the turntable 34 is rotatably connected to a connecting rod 25 via an axle rod, and the upper end of the connecting rod 25 is fixedly connected to the placement table 5.

[0043] A plurality of placement grooves 17 are provided on the outer wall of the placement table 5. A connecting shaft 15 is rotatably connected to the upper surface of the placement table 5 near the middle position. A central gear 39 is fixedly connected to the outer wall of the connecting shaft 15 near the bottom position. A second motor is installed on the bottom surface of the placement table 5, and the output end of the second motor is connected to the connecting shaft 15. Clamps 18 are installed on the edge positions of the plurality of placement grooves 17 on the upper surface of the placement table 5, and one of the clamps 18 is fixedly connected to the upper surface of the placement table 5, and the other clamp 18 is movably installed on the upper surface of the placement table 5. One end of one of the clamps 18 is fixedly connected to a movable plate 20, and the movable plate 20 is rotatably connected to the placement table 5 through a shaft. The upper surface of the movable plate 20 is fixedly connected with a transmission gear 19 through an axis rod, and the transmission gear 19 and the center gear 39 are meshed with each other. The internal space size of the placement groove 17 matches the test tube 38. After the test tube 38 is placed in the placement groove 17, the inner wall of the placement groove 17 fits tightly with the outer wall of the test tube 38, and with the friction force, the position of the test tube 38 can be initially limited. The upper part of the connecting shaft 15 is a retractable structure. The oscillation barrel 1 moves back and forth up and down, and the upper part of the connecting shaft 15 continuously shrinks and stretches accordingly, which will not hinder the vertical movement of the oscillation barrel 1, and the retractable section of the connecting shaft 15 is engaged with the card strip and can rotate synchronously.

[0044] When in use, the cells to be cultured are transferred into the test tube 38, and after the culture solution is injected and sealed, it is clamped in the placement groove 17 opened on the placement table 5. Similarly, multiple test tubes 38 with single cells and culture solution can be clamped in other placement grooves 17 in sequence, and at this time, the test tube 38 is placed close to one of the clamping plates 18 fixed on the upper surface of the placement table 5, and then the connecting shaft 15 is driven to rotate by the second motor, thereby driving the central gear 39 to rotate, and can simultaneously drive each transmission gear 19 to rotate, thereby driving the movable plate Another clamping plate 18 connected to 20 is rotated to a position where its inner wall is close to the outer wall of the test tube 38, so as to clamp and fix the test tube 38. Similarly, several other test tubes 38 can be clamped and fixed at the same time, and then the eccentric wheel 11 is driven to rotate by the motor 1. The centrifugal force generated by the rotation of the eccentric wheel 11 causes the oscillation barrel 1 to reciprocate left and right through the slide rod 7. The two slide rods 7 continuously slide left and right on the slide rod 7, and the buffer spring is continuously compressed and stretched accordingly, so that the oscillation barrel 1 generates a lateral vibration force, and each test tube 38 is vibrated laterally.

[0045] When the eccentric wheel 11 rotates, the bevel gear 26 rotates accordingly, driving the two bevel gears 1 23 to rotate, thereby driving the turntable 34 connected by the two short shafts 22 to rotate, so that one end of the connecting rod 1 24 rotates with the turntable 34 and moves in a circular trajectory, and the other end of the connecting rod 1 24 rotates with the connecting rod 2 25, and can drive the connecting rod 2 25 to reciprocate in the vertical direction, thereby driving the placement table 5 to reciprocate up and down, generating a vertical vibration force, and vibrating each test tube 38 vertically;

[0046] Through the above operation, the test tube 38 can be vibrated horizontally and vertically at the same time, so that the cells and the culture solution can be fully oscillated and fused.

[0047] The upper end of the connecting shaft 15 is rotatably connected to the ratchet 16 through the shaft rod, and the bottom surface of the ratchet 16 is provided with a limiting groove 30. The outer wall of the connecting shaft 15 is rotatably connected to the limiting block 31 located inside the limiting groove 30, and the limiting block 31 and the limiting groove 30 are meshed with each other. The upper end surface of the housing 2 is fixedly connected to the bracket 6, and the upper end surface of the ratchet 16 is fixedly connected to the transmission shaft 40, and the upper end of the transmission shaft 40 is rotatably connected to the top position inside the bracket 6. The outer wall of the housing 2 is rotatably connected to the long shaft 27, and the long shaft A transmission belt 2 33 is sleeved between the vertical axis 27 and the outside of the transmission shaft 40, and a transmission belt 1 32 is sleeved between the vertical axis 28 and the outside of the long axis 27. The connection shaft 15 rotates to drive the clamping plate 18 to rotate toward the test tube 38. When the test tube 38 is clamped, the limit block 31 rotates accordingly, but is restricted by the shape of the limit groove 30 and cannot drive the ratchet 16 to rotate. When the connection shaft 15 rotates in the opposite direction to drive the clamping plate 18 to rotate away from the test tube 38, the limit block 31 cooperates with the limit groove 30 to drive the ratchet 16 to rotate.

[0048] Two frames 10 are fixedly connected to the inner wall of the outer shell 2 and the lower position of the oscillation barrel 1. Slide plates 35 are slidably connected inside the two frames 10, and the upper ends of the two slide plates 35 are fixedly connected to the oscillation barrel 1. When the oscillation barrel 1 moves back and forth left and right, the two slide plates 35 follow the movement and move along the inside of the two frames 10, thereby playing a load-bearing and position-limiting role for the oscillation barrel 1.

[0049] The clamping mechanism includes a connecting rod 29, which is fixedly connected to the upper surface of the vertical shaft 28. One end of the connecting rod 29 is matched with the bottom end of the slide 35. Both outer walls of the slide 35 are provided with movable grooves near the bottom. Blocks 36 are inserted into the two movable grooves. Both inner walls of the connecting rod 29 are provided with clamping grooves 37, and the clamping grooves 37 are matched with the clamping blocks 36. A limiting spring is installed between one end of the clamping block 36 and the inner wall of the movable groove.

[0050] Specifically, after each test tube 38 is automatically oscillated, the connecting shaft 15 is reversed by the second motor, which can drive the clamping plate 18 to rotate away from the test tube 38, release the clamping fixation of the test tube 38, and then put each single cell after each oscillation and fusion into each culture dish 9 in turn, and add the corresponding culture fluid to transfer the single cell to the culture dish 9 for culture. During the rotation of the clamping plate 18 to release the clamping fixation of the test tube 38, the limit block 31 rotates with the connecting shaft 15, cooperates with the limit groove 30, can drive the ratchet 16 to rotate, and the transmission shaft 40 rotates accordingly, thereby driving the transmission belt 2 33 to transmit, and then driving the long shaft 27 to rotate, so that the transmission belt 1 32 transmits, driving the vertical shaft 28 to rotate, and the connecting rod 29 in the clamping mechanism rotates accordingly, so that the end of the connecting rod 29 away from the vertical shaft 28 is clamped and fixed with the bottom of the slide plate 35;

[0051] During the engagement process, the inclined surfaces of the two blocks 36 contact the inner wall of the connecting rod 29, and gradually shrink into the movable groove under the action of the connecting rod 29 rotating and engaging, and the limit spring is compressed. When one end of the connecting rod 29 is completely engaged with the bottom of the slide plate 35, one end of the block 36 is directly opposite to the side of the slot 37. Under the action of the rebound force of the limit spring, one end of the block 36 can be inserted into the slot 37 to fix the position between the connecting rod 29 and the slide plate 35, so that the vertical shaft 28 is connected to the slide plate 35. At this time, the eccentric wheel 11 is driven to rotate by the motor 1, so that the oscillation barrel 1 moves back and forth left and right. The long shaft 27 is manually rotated to rotate the connecting rod 29 to rotate the connecting rod 29 to the initial position, thereby releasing the connection with the slide plate 35, so that when another group of test tubes 38 are subsequently oscillated, each culture dish 9 will not be affected.

[0052] A working method of a single cell culture device, the specific steps are as follows:

[0053] Step 1: Transfer the cells to be cultured into the test tube 38, inject the culture solution and seal it, then clamp it into the placement slot 17 provided on the placement table 5. Similarly, clamp multiple test tubes 38 containing single cells and culture solution into other placement slots 17 in sequence;

[0054] Step 2: The connecting shaft 15 is driven to rotate by the second motor, thereby driving the central gear 39 to rotate, and driving each transmission gear 19 to rotate, and then driving the clamping plate 18 to rotate until its inner wall is close to the outer wall of the test tube 38, clamping and fixing the test tube 38, and clamping and fixing several other test tubes 38 at the same time;

[0055] Step 3: The eccentric wheel 11 is driven to rotate by the motor 1, so that the oscillation barrel 1 generates a lateral vibration force, and each test tube 38 is vibrated laterally. When the eccentric wheel 11 rotates, the bevel gear 26 rotates accordingly, driving the two bevel gears 1 23 to rotate, so that the turntable 34 rotates, and the connecting rod 1 24 and the connecting rod 2 25 are coordinated to make the placement table 5 move up and down reciprocatingly, generating a vertical vibration force, and vibrating each test tube 38 vertically. The test tube 38 is vibrated laterally and vertically at the same time, so that the cells and the culture solution are fully vibrated and fused;

[0056] Step 4: The connecting shaft 15 is driven to reverse by the motor 2 to release the clamping fixation of the test tube 38, and then the single cells after each oscillation and fusion are sequentially put into each culture dish 9, and the corresponding culture medium is added to transfer the single cells to the culture dish 9 for culture;

[0057] Step 5: The end of the connecting rod 29 away from the vertical axis 28 is engaged and fixed with the bottom of the slide 35, and then the eccentric wheel 11 is driven by the motor 1 to rotate, so that the oscillation barrel 1 moves back and forth, and the single cells and culture fluid in each culture dish 9 placed on the circular plate 1 8 are automatically oscillated left and right, and finally left to stand, completing the culture.

[0058] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A single cell culture device, comprising an oscillating barrel (1) and a carrier (3), characterized in that: The upper surface of the carrier (3) is fixedly mounted with a shell (2), the outer wall of the shell (2) is inserted with two slide bars (7), and one end of the two slide bars (7) is fixedly connected to the oscillation barrel (1), and a buffer spring is sleeved outside the two slide bars (7) and located between the outer wall of the oscillation barrel (1) and the inner wall of the shell (2), and a circular plate (12) is fixedly mounted near the bottom of the oscillation barrel (1), and a placing platform (5) is movably mounted above the circular plate (12), and the bottom surface of the placing platform (5) is in contact with the circular plate (12). An auxiliary mechanism is arranged between the upper end surfaces; the bottom surface of the placing platform (5) is fixedly connected to a ring plate (14); the upper end surface of the ring plate (14) is provided with a plurality of circular slots, each of which is provided with a test tube (38); the upper end surface of the carrier (3) is fixedly mounted with a round plate (8); the upper end surface of the round plate (8) is fixedly mounted with a plurality of culture dishes (9); the upper end surface of the round plate (8) is rotatably connected to a vertical shaft (28) near the middle position; the upper end surface of the vertical shaft (28) is provided with a clamping mechanism.

2. A single cell culture device according to claim 1, characterized in that: The bottom end of the oscillating barrel (1) is rotatably connected to an eccentric wheel (11) via an axle rod; a motor 1 is installed on the bottom surface of the oscillating barrel (1), and the output end of the motor 1 is connected to the eccentric wheel (11); two telescopic push rods (13) are fixedly connected to the upper end surface of the circular plate 2 (12), and the upper ends of the two telescopic push rods (13) are fixedly connected to the placement table (5); and the outsides of the two telescopic push rods (13) are both sleeved with support springs.

3. A single cell culture device according to claim 2, characterized in that: The auxiliary mechanism comprises a second bevel gear (26) and two mounting plates (21); the second bevel gear (26) is rotatably connected to the upper end surface of the second circular plate (12) via a shaft; both mounting plates (21) are fixedly mounted on the upper end surface of the second circular plate (12); and the second bevel gear (26) is connected to the eccentric wheel (11) via a shaft.

4. A single cell culture device according to claim 3, characterized in that: The outer walls of the two mounting plates (21) are rotatably connected to a short shaft (22), one end of the short shaft (22) is fixedly connected to a bevel gear 1 (23), the bevel gear 1 (23) and the bevel gear 2 (26) are meshed with each other, the other end of the short shaft (22) is fixedly connected to a turntable (34), the outer wall of the turntable (34) is rotatably connected to a connecting rod 1 (24) via an axle rod, the end of the connecting rod 1 (24) away from the turntable (34) is rotatably connected to a connecting rod 2 (25) via an axle rod, and the upper end of the connecting rod 2 (25) is fixedly connected to the placement table (5).

5. The single cell culture device according to claim 1, characterized in that: The outer wall of the placement platform (5) is provided with a plurality of placement grooves (17); the upper surface of the placement platform (5) is rotatably connected to a connecting shaft (15) near the middle position; the outer wall of the connecting shaft (15) is fixedly connected to a central gear (39) near the bottom position; a second motor is installed on the bottom surface of the placement platform (5), and the output end of the second motor is connected to the connecting shaft (15); The upper surface of the placement table (5) is provided with clamping plates (18) near the edge positions of the plurality of placement grooves (17), and one of the clamping plates (18) is fixedly connected to the upper surface of the placement table (5), and the other clamping plate (18) is movably installed on the upper surface of the placement table (5), one end of one of the clamping plates (18) is fixedly connected to a movable plate (20), and the movable plate (20) is rotatably connected to the upper surface of the placement table (5) via an axle, and the upper surface of the movable plate (20) is fixedly connected to a transmission gear (19) via an axle, and the transmission gear (19) and the center gear (39) are arranged to mesh with each other.

6. A single cell culture device according to claim 5, characterized in that: The upper end of the connecting shaft (15) is rotatably connected to a ratchet (16) via a shaft rod, a limiting groove (30) is provided on the bottom surface of the ratchet (16), and a limiting block (31) is rotatably connected to the outer wall of the connecting shaft (15) and located inside the limiting groove (30), and the limiting block (31) and the limiting groove (30) are meshed with each other.

7. A single cell culture device according to claim 6, characterized in that: The upper end surface of the shell (2) is fixedly connected to a bracket (6), the upper end surface of the ratchet (16) is fixedly connected to a transmission shaft (40), and the upper end of the transmission shaft (40) is rotatably connected to the top position inside the bracket (6), the outer wall of the shell (2) is rotatably connected to a long shaft (27), a second transmission belt (33) is sleeved between the long shaft (27) and the outside of the transmission shaft (40), and a first transmission belt (32) is sleeved between the vertical shaft (28) and the outside of the long shaft (27).

8. The single cell culture device according to claim 1, characterized in that: Two frames (10) are fixedly connected to the inner wall of the outer shell (2) and at a position below the oscillation barrel (1). Slide plates (35) are slidably connected inside the two frames (10), and the upper ends of the two slide plates (35) are fixedly connected to the oscillation barrel (1).

9. The single cell culture device according to claim 1, characterized in that: The clamping mechanism comprises a connecting rod (29), the connecting rod (29) being fixedly connected to the upper surface of the vertical shaft (28), one end of the connecting rod (29) being matched with the bottom end of the slide plate (35), both outer side walls of the slide plate (35) being provided with movable grooves near the bottom, both movable grooves being provided with clamping blocks (36), both inner side walls of the connecting rod (29) being provided with clamping grooves (37), and the clamping grooves (37) being matched with the clamping blocks (36), and a limiting spring being installed between one end of the clamping block (36) and the inner wall of the movable groove.

10. A working method of a single cell culture device according to any one of claims 1 to 9, characterized in that: The specific steps of the working method are as follows: Step 1: Transfer the cells to be cultured into a test tube (38), inject the culture solution into the test tube and seal it, then clamp it into a placement slot (17) provided on the placement table (5). Similarly, clamp multiple test tubes (38) containing single cells and culture solution into other placement slots (17) in sequence. Step 2: The connecting shaft (15) is driven to rotate by the second motor, thereby driving the central gear (39) to rotate, and at the same time driving each transmission gear (19) to rotate, thereby driving the clamping plate (18) to rotate to a position where its inner wall is close to the outer wall of the test tube (38), clamping and fixing the test tube (38), and similarly clamping and fixing several other test tubes (38) at the same time; Step 3: The eccentric wheel (11) is driven to rotate by the motor 1, so that the oscillation barrel (1) generates a lateral vibration force, and each test tube (38) is vibrated laterally. When the eccentric wheel (11) rotates, the bevel gear 2 (26) rotates accordingly, driving the two bevel gears 1 (23) to rotate, so that the turntable (34) rotates, and the connecting rod 1 (24) and the connecting rod 2 (25) are coordinated to make the placement table (5) move up and down reciprocatingly, generating a vertical vibration force, and vibrating each test tube (38) vertically. The test tube (38) is vibrated laterally and vertically at the same time, so that the cells and the culture medium are fully vibrated and fused; Step 4: The connecting shaft (15) is driven to reverse by the second motor to release the clamping of the test tube (38), and then the single cells after each oscillation and fusion are sequentially put into each culture dish (9), and the corresponding culture medium is added to transfer the single cells to the culture dish (9) for culture; Step 5: The end of the connecting rod (29) away from the vertical axis (28) is engaged and fixed with the bottom of the slide plate (35), and then the eccentric wheel (11) is driven to rotate by the motor 1, so that the oscillation barrel (1) moves back and forth left and right, and the single cells and culture fluid in each culture dish (9) placed on the circular plate 1 (8) are automatically oscillated left and right, and finally left to stand to complete the culture.

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