Time-resolved fluorescence immunoassay analyzer

Abstract

The invention discloses a time-resolved fluorescence immunoassay analyzer, which comprises a base, a sample introduction module, an incubation module, a cleaning module and a chip detection module, wherein a chip loading module and a liquid path and gas path module are arranged in the sample injection module; then, the chip is transferred into the incubation module; the incubation module enables the chip to react at a constant temperature within a set temperature; the incubation module freely moves up and down in the vertical direction, and the sample injection module, the cleaning module and the chip detection module are respectively arranged on the side surfaces of the incubation module in the vertical movement direction; the incubation module sends the cleaned chip in the cleaning module to the chip detection module for detection; the cleaning module comprises a U-shaped shifting handle assembly and a cleaning module gas-liquid path assembly; the chip detection module comprises an optical path detection assembly and a detection shifting handle assembly. The modules are arranged in the vertical direction, the overall structure is compact, and the occupied area is small; the modules are smooth and simple in function cooperation, and the detection efficiency is high.

Description

[0001] This application is a divisional application of the Chinese invention patent application with application number "202010444390.7", application date "May 22, 2020", and invention name "Time-resolved fluorescence immunoassay analyzer". Technical Field

[0002] The invention belongs to the technical field of medical equipment, and in particular relates to a time-resolved fluorescence immunoassay analyzer for fluorescence immunoassay based on a microfluidic chip project. Background Art

[0003] Microfluidic chip is a hot area in the development of Miniaturized Total Analysis Systems. Microfluidic chip analysis uses chips as operating platforms, analytical chemistry as its basis, micro-electromechanical processing technology as its support, micro-pipeline networks as its structural features, and life sciences as its main application objects. It is the focus of the current development of the field of micro-total analysis systems. Its goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microchip. Microfluidic chip is the main platform for the realization of microfluidic technology. The main feature of its device is that its effective structure (channels, reaction chambers and certain other functional components) that contains fluids is at the micron scale in at least one dimension. Due to the micron-scale structure, the fluid displays and produces special properties that are different from the macroscopic scale. Therefore, a unique analysis performance is developed. Characteristics and development advantages of microfluidic chips: Microfluidic chips have the characteristics of controllable liquid flow, minimal consumption of samples and reagents, and analysis speed increased by ten or even a hundred times. It can analyze hundreds of samples simultaneously in a few minutes or even shorter time, and can realize the whole process of sample pretreatment and analysis online. The purpose of its application is to realize the ultimate goal of micro-total analysis system - chip laboratory. The key application field of current work development is the field of life sciences.

[0004] Current international research status: innovation is mostly focused on separation and detection systems; research on many issues such as how to introduce actual sample analysis on the chip, such as sample introduction, sample replacement, pre-treatment, etc. is still very weak. Its development depends on the development of multidisciplinary cross-disciplinary.

[0005] In Chinese patent document CN108414522A, a device for a microfluidic chip and NC membrane multi-flux detection integrated machine is disclosed, comprising a base, on which an injection module, an incubation module, a cleaning module, an NC membrane detection module and a chip detection module are arranged; wherein the injection module is provided with a mutually independent chip injection card slot and an NC membrane injection card slot, which are respectively used to insert a chip or an NC membrane card strip; the injection module is also provided with a liquid and gas path module; the incubation module has a mutually independent chip incubation groove and an NC membrane incubation groove, and the injection module, the cleaning module and the NC membrane detection module are arranged along the side of the incubation module in the length direction; the chip cleaned in the cleaning module is sent to the chip detection module for detection.

[0006] However, the above technical solution arranges the various components along the sides of the incubation module in the length direction and spreads them out flat, which takes up too much space and makes the equipment appear bloated during use; in addition, the connection between the modules needs to be further improved, and the coordination is not very smooth. Summary of the invention

[0007] The technical problem to be solved by the present invention is to provide a time-resolved fluorescence immunoassay analyzer with a compact overall structure, a small footprint, a vertical arrangement, and smooth and simple functional coordination of each module.

[0008] In order to solve the above technical problems, the technical solution adopted by the present invention is that the time-resolved fluorescence immunoassay analyzer includes a base, a sample injection module, an incubation module, a cleaning module and a chip detection module;

[0009] The sample injection module is provided with a chip loading module and a liquid and gas path module, wherein the chip loading module is used to carry the chip to the liquid and gas path module, and the liquid and gas path module is used to make the sample in the chip flow to a predetermined position; and then the chip is transported to the incubation module;

[0010] The incubation module allows the chip to react at a constant temperature within a set temperature;

[0011] The incubation module freely moves up and down in the vertical direction, and the injection module, cleaning module and chip detection module are respectively arranged on the side of the vertical movement direction of the incubation module; the incubation module sends the chip cleaned in the cleaning module to the chip detection module for detection.

[0012] The liquid and gas path modules in the injection module are used to make the sample in the chip flow to a predetermined position to ensure that the chip reaction chamber is filled with samples and reagents for reaction; the above technical solution is adopted to integrate the various module components on the base, and respectively arranged on the sides of the vertical movement direction of the incubation module to form a complete set of time-resolved fluorescence immunoassay equipment, which has an integrated structure, a compact overall structure, and a small footprint. It is arranged in a vertical direction to avoid wasting space by laying out the equipment, and the functions of the various modules in the above technical solution are coordinated smoothly and conveniently; the injection module, the cleaning module and the chip detection module are all arranged around the incubation module, and the chip turnover is carried out with the incubation module as the center.

[0013] During the specific operation: the chip to be tested is placed in the analyzer, and then the chip is driven by the chip loading module to move to the liquid and gas module, so that the sample in the chip injection chamber slowly moves along the chip microchannel and flows to the predetermined position; the chip is moved to the incubation module so that the chip reaction chamber performs sample immune reaction at a preset temperature; after the reaction is completed, the chip is moved to the cleaning module, which can clean the chip after the reaction. After the chip cleaning is completed, the chip is moved to the incubation module, and the incubation module sends the chip to the chip detection module for detection. The incubation module rises to the corresponding position of the chip detection module. The test results can be displayed on the instrument display screen, and can also be printed through the built-in printer.

[0014] The present invention is further improved in that the incubation module has an incubation tank assembly, the incubation tank assembly includes a heating plate and a heat preservation plate; the incubation tank assembly includes a plurality of stacked incubation tanks, and the plurality of stacked incubation tanks can freely move up and down along the incubation tank slide rail. The incubation tank assembly is provided with a heating plate and a heat preservation plate to enable the chip to react at a constant temperature within a set temperature.

[0015] A further improvement of the present invention is that the incubation trough assembly is also provided with an incubation trough connecting block; the incubation trough connecting block is connected to the incubation trough motion belt, the brake motor drives the incubation trough motion active wheel, and the incubation trough motion belt drives the incubation trough motion driven wheel to rotate, so that the multiple stacked incubation troughs in the incubation trough assembly can move freely up and down along the incubation trough slide rail.

[0016] The present invention is further improved in that the incubation trough slide rail is arranged on the incubation trough slide rail bracket, and a buffer block is arranged on the side of the incubation trough assembly, and a buffer pad is arranged at the bottom of the incubation trough slide rail bracket accordingly; a limit baffle is arranged on the incubation trough assembly, and the incubation trough slide rail bracket is fixedly connected to the limit optical coupling connection block, and the limit optical coupling is installed on the limit optical coupling connection block, which is used to limit the up and down movement of the incubation trough. A buffer block is arranged on the side of the incubation trough assembly, and a buffer pad is arranged at the bottom of the corresponding incubation trough slide rail bracket to provide buffering when the incubation trough moves downward to prevent damage. At the same time, a limit baffle is arranged on the incubation trough assembly, and the incubation trough slide rail bracket is fixedly connected to the limit optical coupling connection block, and the limit optical coupling is installed on the limit optical coupling connection block, which is used to limit the up and down movement of the incubation trough.

[0017] Preferably, the incubation tank is provided with a chip pressing strip, and two protrusions of the chip pressing strip cooperate with the grooves in the chip to position the chip for incubation reaction.

[0018] Preferably, the heating plates and the heat-insulating plates are both arranged on the sides of the plurality of stacked incubation tanks.

[0019] A further improvement of the present invention is that the chip loading module includes a chip sampling lever, which moves the chip to put it into place in the chip sampling slot; a chip limiting piece structure is arranged behind the chip sampling slot; and the chip moves driven by the chip sampling slot.

[0020] Preferably, the chip sampling dial hand is controlled by a chip sampling dial hand motion component; the chip sampling dial hand motion component includes a sampling dial hand horizontal motion component and a sampling dial hand up and down motion component; the sampling dial hand horizontal motion component has a sampling dial hand horizontal motion slider, the sampling dial hand horizontal motion slider moves along the sampling dial hand horizontal slide rail, the sampling dial hand horizontal motion slider is connected to the sampling dial hand horizontal motion slider connecting block, the sampling dial hand horizontal motion slider connecting block is fixedly connected with the chip sampling dial hand up and down motion slide rail, the sampling dial hand up and down motion slider is connected to the chip sampling dial hand up and down motion slide rail, the sampling dial hand up and down motion slider is connected to the chip sampling dial hand, the chip sampling dial hand is provided with a chip sampling dial hand slide rod, and the chip sampling dial hand slide rod moves in the chip sampling dial hand slide groove.

[0021] Preferably, the chip sampling slot is controlled by the sampling slot motion motor, and the sampling slot motor nut moves along the sampling slot lead screw, the sampling slot motor nut is connected to the sampling slot connecting block 1, the sampling slot connecting block 1 is connected to the chip sampling slot, the sampling slot connecting block 2 is connected to the sampling slot connecting block 1, the sampling slot connecting block 2 is connected to the sampling slot slider, and the sampling slot slider reciprocates along the sampling slot slide rail.

[0022] Preferably, there are two injection slot slide rails on the partition of the chip loading module, namely injection slot slide rail 1 and injection slot slide rail 2; accordingly, there are two injection slot sliders, namely injection slot slider 1 and injection slot slider 2, and there are two injection slot connecting blocks 2, namely first injection slot connecting block 2 and second injection slot connecting block 2; wherein, the first injection slot connecting block 2 is connected to the injection slot slider 1, and the injection slot slider 1 reciprocates along the injection slot slide rail 1, and the second injection slot connecting block 2 is connected to the injection slot slider 2, and the injection slot slider 2 reciprocates along the injection slot slide rail 2.

[0023] Preferably, the partition is provided with a chip loading module bracket 1 and a chip loading module bracket 2, wherein the chip loading module bracket 2 is provided with a chip limiting plate slide; the chip limiting plate structure has a chip limiting plate, and the chip limiting plate is provided with a chip limiting plate slide rod for moving in the chip limiting slide; a chip limiting plate slide rail is fixedly connected to the side wall of the chip feeding slot, and a chip limiting plate slider is connected to the chip limiting plate slide rail, and the chip limiting plate is connected to the chip limiting plate slider, and the chip limiting plate moves horizontally with the chip feeding slot and cooperates with the chip limiting plate slide groove to move up and down. In this technical solution, the slide groove of the chip loading module bracket 1 has no specific function, and is unified with the structure of the chip loading module bracket 2 for easy processing and manufacturing.

[0024] Preferably, the front end of the sampling dial hand slide is higher than the rear end, so that the sampling dial hand slide has a high starting point; the sampling dial hand horizontal motion component has a sampling dial hand horizontal motor, one end of the sampling dial hand belt connecting block is fixedly connected to the sampling dial hand horizontal belt, and the other end of the sampling dial hand belt connecting block is fixedly connected to the sampling dial hand horizontal motion slider connecting block.

[0025] Preferably, the sampling dial hand horizontal motion assembly further comprises a sampling dial hand motion driving wheel and a sampling dial hand motion driven wheel, the sampling dial hand horizontal belt is sleeved on the sampling dial hand motion driving wheel and the sampling dial hand motion driven wheel, the sampling dial hand driving wheel is connected to the sampling dial hand horizontal motor, and the sampling dial hand horizontal motion slider connecting block is sleeved on the sampling dial hand horizontal belt. The sampling dial hand horizontal motor is started, and the chip sampling dial hand moves along the sampling dial hand horizontal slide rail, and the initial position of the sampling dial hand is above the front end of the chip sampling slot (the high starting point of the sampling dial hand slide slot). After the chip is placed in, the chip insertion sensor senses the chip, and the sampling dial hand moves along the sampling dial hand slide slot, and the front end of the sampling dial hand slide slot is set to be higher than the rear end, so as not to block the chip from being placed in the chip sampling slot. The sampling dial hand moves the chip so that the chip completely reaches the preset position of the sampling slot to prevent the chip from being placed in place.

[0026] A further improvement of the present invention is that the liquid circuit and gas circuit module includes a liquid circuit and gas circuit control motor and a chip valve control motor; the liquid circuit and gas circuit control motor drives the annular bracket to move downward, and the annular bracket is pressed downward so that the injection port interface located on the annular bracket is sealed to the injection chamber of the docking chip through the injection port sealing head, and the external gas-liquid port interface is sealed to the external gas-liquid port of the docking chip through the external gas-liquid port sealing joint; a liquid detection rod is fixedly connected to the annular bracket, and the liquid detection rod is connected to the liquid detection sensor; the chip valve control motor drives the chip valve slider connecting block to move, and a chip valve pressure rod is installed on the chip valve slider connecting block, and the chip valve pressure rod presses down the chip valve under the control of the chip valve control motor.

[0027] Preferably, the liquid and gas circuit module also includes a liquid and gas circuit rail and a liquid and gas circuit slider; the liquid and gas circuit control motor is started, the liquid and gas circuit screw rod rotates, and the liquid and gas circuit motor nut on the liquid and gas circuit screw rod drives the annular bracket to move downward, and the annular bracket is connected to the liquid and gas circuit rail and moves in coordination with the liquid and gas circuit slider, and the liquid and gas circuit slider is fixedly connected to the inner side of the side bracket of the liquid and gas circuit module.

[0028] Preferably, the liquid-gas circuit module also includes a chip valve slide rail and a chip valve slider; the chip valve control motor is started, the chip valve screw rotates, the chip valve motor nut on the chip valve screw drives the chip valve slider connection block to move, the chip valve slider connection block is connected to the chip valve slider, and the chip valve slide rail is installed on the inner side of the support plate of the liquid-gas circuit module. The liquid-gas circuit control motor is started, the liquid-gas circuit screw rotates, the liquid-gas circuit motor nut drives the annular bracket to move downward, the annular bracket is connected to the liquid-gas circuit slide rail, and moves with the liquid-gas circuit slider, the liquid-gas circuit slider is fixedly connected to the inner side of the side bracket, the annular bracket is pressed down so that the injection port interface (air intake pressurization effect) located on the annular bracket is sealed to the injection cavity of the docking chip through the injection port sealing head, the external gas-liquid port interface (sealed treatment, gas and liquid cannot enter) is sealed to the external gas-liquid port of the docking chip through the external gas-liquid port sealing joint, and then the air intake pressurization is performed through the injection port interface to make the sample in the chip injection cavity slowly move along the chip microchannel. A liquid detection rod is fixedly connected to the annular bracket, and the liquid detection rod is connected to the liquid detection sensor. When the sample in the chip reaches the chip conductive rubber valve, the liquid detection rod senses the capacitance change, the chip valve control motor of the instrument starts, the chip valve screw rotates, and the chip valve motor nut drives the chip valve slider connecting block to move. The chip valve slider connecting block is connected to the valve slider, the chip valve slide rail is installed on the inner side of the support plate, and the chip valve pressure rod is installed on the chip valve slider connecting block. The chip valve pressure rod presses down the valve under the control of the chip valve control motor, so that the chip liquid cannot continue to move forward, and at the same time, the injection port interface stops air intake and pressurization.

[0029] Preferably, the liquid circuit and gas circuit module is also provided with a chip valve limiting optical coupler and a chip valve limiting baffle for limiting the height of the chip valve pressure rod descending; the liquid circuit and gas circuit module is also provided with a liquid circuit and gas circuit limiting optical coupler and a liquid circuit and gas circuit limiting baffle for limiting the height of the annular bracket descending.

[0030] Preferably, the liquid and gas path module further includes a chip scanner for scanning the QR code on the chip. The chip scanner is located above the chip and scans the chip through the through hole on the top plate of the injection module to confirm whether the chip detection items are correct and whether the chip is within the validity period. If it does not meet the requirements, the machine alarms and prompts an error message.

[0031] A further improvement of the present invention is that the cleaning module includes a U-shaped finger assembly and a cleaning module gas-liquid circuit assembly; after the chip incubation reaction is completed, the U-shaped finger in the U-shaped finger assembly moves the chip to the cleaning position in the cleaning module gas-liquid circuit assembly for cleaning; after the chip cleaning is completed, the U-shaped finger moves the chip back.

[0032] Preferably, the U-shaped dial hand assembly is provided with a U-shaped dial hand control motor. Under the control of the U-shaped dial hand control motor, the U-shaped dial hand driving wheel rotates, and the U-shaped dial hand driven wheel is driven by the U-shaped dial hand belt. The U-shaped dial hand belt connecting block on the U-shaped dial hand belt is connected to the U-shaped dial hand slider assembly, and the U-shaped dial hand slider assembly is connected to the U-shaped dial hand and moves along the U-shaped dial hand slide rail.

[0033] Preferably, the U-shaped push arm extends to two sides to form a U-shaped push arm 1 and a U-shaped push arm 2, respectively, and the distance between the U-shaped push arm 1 and the U-shaped push arm 2 is greater than the length of the chip.

[0034] Preferably, the cleaning module gas-liquid circuit assembly has a cleaning module control motor, when the cleaning module control motor is started, the cleaning module screw rotates, and the cleaning module motor nut on the cleaning module screw drives the cleaning module annular bracket to move up and down; the cleaning module annular bracket is fixedly connected to the cleaning module slide rail, and the cleaning module slide rail is slidably connected to the cleaning module slider; the cleaning module annular bracket is provided with a cleaning module liquid detection rod and a cleaning module gas-liquid circuit interface, and the cleaning module gas-liquid circuit interface is a cleaning liquid interface and an external gas circuit interface respectively, and the cleaning liquid interface and the external gas circuit interface correspond to the cleaning module external gas-liquid port sealing joint at the bottom of the cleaning module annular bracket; the cleaning module liquid detection rod is connected to the cleaning module liquid detection sensor.

[0035] Preferably, the cleaning module gas-liquid circuit assembly is also provided with a cleaning module limit optical coupler and a cleaning module limit baffle, which are used to limit the height of the cleaning module annular bracket; the cleaning module chip inlet sealing joint is also provided at the bottom of the cleaning module annular bracket, and the cleaning module chip inlet sealing joint is located on the side of the cleaning module external gas-liquid port sealing joint. The cleaning module control motor is started, and the cleaning module annular bracket can move up and down. When the chip is cleaned, the cleaning module annular bracket is pressed down, and the two sealing joints (the cleaning module external gas-liquid port sealing joint and the chip inlet sealing joint) are respectively sealed and combined with the corresponding ports on the chip. When the cleaning liquid is added, the liquid detection rod stops adding liquid when the liquid is detected, and the external gas circuit intake is pressurized to make the cleaning liquid flow into the waste liquid chamber, and the cleaning is repeated 3-4 times. The cleaned chip is moved to the incubation tank by the U-shaped dial hand, and the incubation tank rises to the corresponding position of the chip detection module, and is moved to the detection position by the detection dial hand. The detection position is located on the chip carrier, and the carrier moves so that the optical path detection component detects the chip.

[0036] The present invention is further improved in that the chip detection module includes an optical path detection component and a detection dial component; the detection dial in the detection dial component moves the chip to the detection position, the detection position is located on the chip carrier, and the chip carrier moves so that the optical path detection component detects the chip; the chip detection module is provided with an upper shielding cover and a lower shielding cover. Since light protection is required during detection, the chip detection module is provided with two shielding covers, an upper shielding cover and a lower shielding cover, to reduce the influence of ambient light on the detection results.

[0037] Preferably, the detection dial hand assembly has a detection dial hand control motor, the detection dial hand control motor drives the detection dial hand control motor driving wheel to rotate, and the detection dial hand control motor driving wheel drives the detection dial hand control motor driven wheel through the detection dial hand control motor belt; the detection dial hand is connected to the detection dial hand slider connecting block, the detection dial hand slider connecting block is fixedly connected with the detection dial hand slider, and the detection dial hand slider and the detection dial hand move along the detection dial hand slide rail.

[0038] Preferably, a detection dial limit plate is provided at one end of the detection dial, and a detection dial limit optical coupler connection block arranged on the bottom plate of the chip detection module is fixedly connected to the detection dial limit optical coupler for limiting the movement of the detection dial.

[0039] Preferably, the optical path detection component has a light-collecting head, and a transmitting plate is arranged on the upper part of the light-collecting head; a receiving plate is arranged at the lower part of the detection position, and the transmitting plate emits laser, which passes through the light-collecting head to perform fluorescence excitation on the reactants in the chip reaction cavity located at the detection position, and then is received by the receiving plate, and the detection result is calculated after the fluorescence is collected and amplified.

[0040] Preferably, below the transmitting plate, a first filter, a first lens, a second lens and a second filter are arranged in sequence from top to bottom; the receiving plate is arranged below the second filter; wherein the first filter and the first lens are part of the daylighting head; the daylighting head is connected to the daylighting head fixing block, and the daylighting head fixing block is connected to the bottom plate of the chip detection module.

[0041] Preferably, a first light shielding block and a second light shielding block are respectively provided on the sides of the second lens and the second filter; the second light shielding block shields the ambient light from the chip disposal channel and serves as a connecting block of the chip disposal channel.

[0042] Preferably, the chip carrier is driven by a chip carrier control motor, and the chip carrier control motor drives the chip carrier lead screw to rotate, and the chip carrier motor nut connecting block on the chip carrier lead screw moves; the chip carrier motor nut connecting block is connected to the chip carrier, and the chip carrier is connected to a chip carrier slider 1 and a chip carrier slider 2; the chip carrier slider 1 and the chip carrier slider 2 drive the chip carrier to move along the chip carrier slide rail.

[0043] Preferably, the chip detection module is also provided with a discarding dial hand for transferring the detected chip into the chip disposal channel; the discarding dial hand is driven by a discarding dial hand control motor, the discarding dial hand slider is fixed on the top plate of the chip detection module, and the discarding dial hand slide rail is connected to the discarding dial hand motor nut connecting block; the discarding dial hand control motor drives the discarding dial hand screw to rotate, and the discarding dial hand motor nut connecting block moves along the discarding dial hand screw, and the discarding dial hand motor nut connecting block is connected to the vertical plate, and the vertical plate is connected to the horizontal plate; one end of the discarding dial hand is connected to the horizontal plate as a discarding dial hand fixing part, and the other end of the discarding dial hand is used as a discarding dial hand moving part for moving the chip into the chip disposal channel.

[0044] Preferably, a disposal dial hand limit block is fixed on the disposal dial hand motor nut connecting block, and the disposal dial hand sensing portion of the disposal dial hand limit block cooperates with a disposal dial hand limit sensor installed on the top plate to limit the disposal dial hand.

[0045] Preferably, two disposal handles are provided, which are respectively arranged on both sides of the horizontal plate.

[0046] Preferably, the exterior of the time-resolved fluorescence immunoassay analyzer is also covered with a shell, on which a display screen is provided, and all detection-related contents can be viewed on the display screen; an inlet is provided on the shell for inserting the chip, and a disposal groove is also provided on the side of the shell; the time-resolved fluorescence immunoassay analyzer is also provided with a built-in printer, and a paper outlet of the built-in printer is provided on the shell.

[0047] Compared with the prior art, the technical solution of the present invention has the following beneficial effects: the modules are arranged in a vertical direction, the overall structure is compact, and the footprint is small; and the functions of the modules are coordinated smoothly and simply, and the detection efficiency is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] The following is further described in detail with reference to the accompanying drawings and embodiments of the present invention:

[0049] Figure 1 It is a schematic diagram of the overall internal structure of the time-resolved fluorescence immunoassay analyzer of the present invention;

[0050] Figure 2 It is a schematic diagram of the overall external structure of the time-resolved fluorescence immunoassay analyzer of the present invention;

[0051] Figure 3 yes Figure 1 Schematic diagram of the injection module structure;

[0052] Figure 4 yes Figure 1 A rear view structural diagram of the injection module in FIG.

[0053] Figure 5 1 is a schematic diagram of the top view of the injection module;

[0054] Figure 6 yes Figure 4 A schematic diagram of the rear view structure of the chip loading module in FIG.

[0055] Figure 7 yes Figure 3 A schematic diagram of the chip loading module in a side upward view;

[0056] Figure 8 yes Figure 3 A partial enlarged schematic diagram of the sample injection hand motion assembly;

[0057] Fig. 9 yes Figure 6 A schematic diagram of the structure of the sample injection dial in FIG.

[0058] Fig.10 yes Figure 3 A partial schematic diagram of the chip limiting piece structure in the injection module;

[0059] Fig.11 yes Figure 8 A partial enlarged schematic diagram of the chip limiting plate structure;

[0060] Fig.12 yes Figure 8 A partially enlarged top view schematic diagram of the chip limiting plate structure;

[0061] Fig.13yes Figure 3 A schematic diagram of the structure of the liquid and gas path modules;

[0062] Fig.14 yes Figure 4 A rear view structural diagram of the liquid and gas path module;

[0063] Fig.15 yes Fig.13 A schematic diagram of the structure of the liquid and gas circuit modules in a side upward view;

[0064] Fig.16 yes Figure 1 A schematic diagram of the structure of the incubation module;

[0065] Fig.17 yes Fig.16 A partial enlarged view of the buffer structure;

[0066] Fig.18 yes Fig.16 A partial enlarged view of the limiting structure in FIG.

[0067] Fig.19 yes Fig.16 A schematic diagram of the structure of the incubation tank assembly;

[0068] Fig. 20 yes Fig.16 A schematic diagram of the structure of the incubation tank;

[0069] Fig.21 is a schematic diagram of the structure of the cleaning module;

[0070] Fig. 22 yes Fig.21 Schematic diagram of the cleaning module-U-shaped hand assembly structure;

[0071] Fig.23 yes Fig.21 The structure diagram of the gas and liquid circuit components of the cleaning module;

[0072] Fig.24 yes Fig.21 A rear view structural diagram of the cleaning gas-liquid assembly;

[0073] Fig.25 It is a schematic diagram of the structure of the chip detection module;

[0074] Fig.26 1 is a schematic diagram of the chip detection module viewed from above;

[0075] Fig. 27 yes Fig.25 A schematic diagram of the structure of the chip detection module with the shielding cover removed;

[0076] Fig.28 It is a cross-sectional view of the optical path components in the chip detection module;

[0077] Fig.29 yes Fig.25 The rear view structure diagram without the shielding cover;

[0078] Fig.30 is a schematic diagram of the chip carrier assembly structure;

[0079] Fig.31 It is a side view of the chip detection module with the shielding cover removed;

[0080] Fig.32 yes Fig.31 A schematic diagram of the structure of the discard handle control assembly;

[0081] Fig.33 yes Fig.32 A top view schematic diagram of the abandoned handle structure;

[0082] Among them: 1-base; 2-injection module; 201-chip loading module; 2011-partition; 2012-chip loading module bracket one; 2013-chip loading module bracket two; 202-liquid and gas path module; 2021-liquid and gas path control motor; 2022-chip valve control motor; 2023-ring bracket; 2024-liquid detection rod; 2025-liquid detection sensor; 2026-external gas and liquid port sealing joint; 2027-chip valve pressure rod; 2028-external gas and liquid port interface; 2029-liquid and gas path slide rail; 20210-liquid and gas path slider; 20211-liquid and gas path screw rod; 20212-liquid and gas path motor nut; 20213-side bracket; 20214-chip Valve rail; 20215-chip valve slider; 20216-chip valve screw rod; 20217-chip valve motor nut; 20218-chip valve slider connection block; 20219-support plate; 20220-chip valve limit optical coupler; 20221-chip valve limit baffle; 20222-liquid and gas path limit baffle; 20223-liquid and gas path limit optical coupler; 20224-injection port interface; 20225-injection port sealing head; 203-chip injection hand; 2031-chip injection hand slide; 2032-injection hand slide; 2033-chip insertion sensor; 204-chip injection slot; 2041-injection slot motion motor; 2042-injection slot motor nut; 2043-injection Slot screw rod; 2044-slot connection block 1; 2045-slot connection block 2; 20451-first slot connection block 2; 20452-second slot connection block 2; 2046-slot slider; 20461-slot slider 1; 20462-slot slider 2; 2047-slot rail; 20471-slot rail 1; 20472-slot rail 2; 205-chip injection hand motion assembly; 2051-slot hand horizontal motion slider; 2052-slot hand horizontal slide; 2053-slot hand horizontal motion slider connection block; 2054-chip injection hand up and down motion slide; 2055-slot hand up and down motion slider; 2056-slot hand horizontal motor ;2057-sampling hand belt connecting block;2058-sampling hand horizontal belt;2059-sampling hand motion driving wheel;20510-sampling hand motion driven wheel;206-chip limit plate structure;2061-chip limit plate;2062-limit plate slide rod;2063-chip limit plate slide groove;2064-chip limit plate slide rail;2065-chip limit plate slider;207-chip barcode scanner;208-top plate;2081-through hole;3-incubation module;301-incubation tank assembly;30101-heating plate;30102-insulation plate;30103-incubation tank;302-incubation tank slide rail;303-incubation tank connecting block;304-brake motor;305-incubation tank motion belt;306-incubation tank motion driving wheel; 307-incubation tank motion driven wheel; 308-incubation tank slide rail bracket; 3081-buffer pad; 309-buffer block; 3010-limiting baffle; 3011-limiting optical coupling connection block; 3012-limiting optical coupling; 3013-chip pressure strip; 3014-nut; 4-cleaning module; 401-cleaning side bracket; 402-cleaning module gas-liquid circuit assembly; 4021-cleaning module control motor; 4022-cleaning module lead screw; 4023-cleaning module motor nut; 4024-cleaning module annular bracket; 4025-cleaning module slide rail; 4026-cleaning module slider; 4027-cleaning module liquid detection rod; 4028-cleaning module gas-liquid circuit interface; 40 281-cleaning liquid interface; 40282-external gas line interface; 4029-cleaning module liquid detection sensor; 40210-cleaning module limit optical coupler; 40211-cleaning module limit baffle; 40212-cleaning module chip injection port sealing joint; 40213-cleaning module external gas and liquid port sealing joint; 403-U-shaped dial hand; 4031-U-shaped dial arm one; 4032-U-shaped dial arm two; 404-U-shaped dial hand control motor; 405-U-shaped dial hand driving wheel; 406-U-shaped dial hand belt; 407-U-shaped dial hand driven wheel; 408-U-shaped dial hand belt connecting block; 409-U-shaped dial hand slider assembly; 4010-U-shaped dial hand slide rail; 5-chip detection module; 501-optical path detection Components; 5011-lighting head; 50111-lighting head fixing block; 5012-transmitting board; 5013-receiving board; 5014-first filter; 5015-first lens; 5016-second lens; 5017-second filter; 5018-first shading block; 5019-second shading block; 502-detection dial assembly; 5021-detection dial control motor; 5022-detection dial control motor driving wheel; 5023-detection dial control motor belt; 5024-detection dial control motor driven wheel; 5025-detection dial slider connecting block; 5026-detection dial slider; 5027-detection dial slide rail; 503-detection dial; 5031-detection dial limiter; 503 2-detection dial limit optical coupler connection block; 5033-detection dial limit optical coupler; 504-chip carrier; 5041-chip carrier control motor; 5042-chip carrier lead screw; 5043-chip carrier motor nut connection block; 5044-chip carrier slider 1; 5045-chip carrier slider 2; 5046-chip carrier slide rail; 505-upper shielding cover; 506-lower shielding cover; 507-bottom plate; 508-chip disposal channel; 509-discard dial; 5091-discard dial control motor; 5092-discard dial slider; 5093-discard dial slide rail; 5094-discard dial motor nut connection block; 5095-discard dial lead screw; 5096-vertical plate; 5097-horizontal plate;5098-discarding hand limit block; 5099-discarding hand moving part; 50100-discarding hand sensing part; 50910-discarding hand fixing part; 5010-top plate; 50101-discarding hand limit sensor; 6-housing; 7-display screen; 8-inlet; 9-discarding slot; 10-built-in printer; 1001-paper outlet. ; DETAILED DESCRIPTION

[0083] like Figure 1 , which is a schematic diagram of the internal overall structure of the time-resolved fluorescence immunoassay analyzer of the present invention, including a base 1, a sample injection module 2, an incubation module 3, a cleaning module 4 and a chip detection module 5;

[0084] The sample injection module 2 is provided with a chip loading module 201 and a liquid and gas path module 202. The chip loading module 201 is used to carry the chip to the liquid and gas path module 202, and the liquid and gas path module 202 is used to make the sample in the chip flow to a predetermined position; then the chip is transported to the incubation module 3;

[0085] The incubation module 3 allows the chip to react at a constant temperature within a set temperature;

[0086] The incubation module 3 freely moves up and down in the vertical direction, and the injection module 2, the cleaning module 4 and the chip detection module 5 are respectively arranged on the side of the vertical movement direction of the incubation module 3; the incubation module 3 sends the chip cleaned in the cleaning module 4 to the chip detection module 5 for detection;

[0087] like Figure 2 As shown, the exterior of the time-resolved fluorescence immunoassay analyzer is also covered with a shell 6, on which a display screen 7 is provided, and all detection-related contents can be viewed on the display screen 7; an injection port 8 is provided on the front of the shell 6 for inserting a chip, and a disposal slot 9 is also provided on the side of the shell 6; the time-resolved fluorescence immunoassay analyzer is also provided with a built-in printer 10, and a paper outlet 1001 of the built-in printer 10 is provided on the shell 6.

[0088] like Figures 3 to 12 As shown, the chip loading module 201 includes a chip sample introduction hand 203, and the chip sample introduction hand 203 moves the chip to put it into place in the chip sample introduction slot 204; a chip limiting piece structure 206 is arranged behind the chip sample introduction slot 204; the chip moves under the drive of the chip sample introduction slot 204;

[0089] like Figure 8As shown, the chip sampling dial 203 is controlled by the chip sampling dial motion component 205; the chip sampling dial motion component includes a sampling dial horizontal motion component and a sampling dial up and down motion component; the sampling dial horizontal motion component has a sampling dial horizontal motion slider 2051, and the sampling dial horizontal motion slider 2051 moves along the sampling dial horizontal slide rail 2052, and the sampling dial horizontal motion slider 2051 is connected to the sampling dial horizontal motion slider connecting block 2053, and the sampling dial horizontal motion slider connecting block 2053 is fixedly connected to the chip sampling dial up and down motion slide rail 2054, and the chip sampling dial up and down motion slide rail 2054 is connected to the sampling dial up and down motion slider 2055, and the sampling dial up and down motion slider 2055 is connected to the chip sampling dial 203, as shown in FIG. Fig. 9 As shown, the chip sampling dial hand 203 is provided with a chip sampling dial hand slide bar 2031, and the chip sampling dial hand slide bar 2031 moves in the chip sampling dial hand slide groove 2032; that is, the chip sampling dial hand 203 can move horizontally and up and down along the sampling dial hand slide groove 2032;

[0090] like Figures 3-4 As shown, the chip sampling slot 204 is controlled by the sampling slot motion motor 2041, and the sampling slot motor nut 2042 moves along the sampling slot lead screw 2043, and the sampling slot motor nut 2042 is connected to the sampling slot connecting block 1 2044, and the sampling slot connecting block 1 2044 is connected to the chip sampling slot 204, and the sampling slot connecting block 2045 is connected to the sampling slot connecting block 1 2044, and the sampling slot connecting block 2045 is connected to the sampling slot slider 2046, and the sampling slot slider 2046 reciprocates along the sampling slot slide rail 2047.

[0091] like Figures 5-6 As shown, there are two injection slot rails 2047 on the partition 2011 of the chip loading module 201, namely, injection slot rail 1 20471 and injection slot rail 2 20472; correspondingly, there are two injection slot sliders 2046, namely, injection slot slider 1 20461 and injection slot slider 2 20462, and there are two injection slot connecting blocks 2 2045, namely, a first injection slot connecting block 2 20451 and a second injection slot connecting block 2 20452; wherein, the first injection slot connecting block 2 20451 is connected to the injection slot slider 1 20452, and the injection slot slider 1 20461 reciprocates along the injection slot rail 1 20471, and the second injection slot connecting block 2 20452 is connected to the injection slot slider 2 20462, and the injection slot slider 2 20462 reciprocates along the injection slot slider 2 20472;

[0092] like Figures 10-12As shown, the partition 2011 is provided with a chip loading module bracket 1 2012 and a chip loading module bracket 2 2013, wherein the chip loading module bracket 2013 is provided with a chip limiting plate slide groove 2063; the chip limiting plate structure 206 has a chip limiting plate 2061, and the chip limiting plate 2061 is provided with a chip limiting plate slide rod 2062 for moving in the chip limiting plate slide groove 2063; a chip limiting plate slide rail 2064 is fixedly connected to the side wall of the chip injection slot 204, and a chip limiting plate slider 2065 is connected to the chip limiting plate slide rail 2064, and the chip limiting plate 2061 is connected to the chip limiting plate slider 2065, and the chip limiting plate 2061 follows the chip injection slot 204 to move horizontally, and cooperates with the chip limiting plate slide groove 2063 to move up and down. In this technical solution, the slide groove of the chip loading module bracket 1 2012 has no specific function and is designed to be unified with the structure of the chip loading module bracket 2 2013 for ease of processing and manufacturing.

[0093] like Figure 6 As shown, the front end of the sampling dial hand slide 2032 is higher than the rear end, so that the sampling dial hand slide 2032 has a high starting point; the sampling dial hand horizontal motion component has a sampling dial hand horizontal motor 2056, and one end of the sampling dial hand belt connecting block 2057 is fixedly connected to the sampling dial hand horizontal belt 2058, and the other end of the sampling dial hand belt connecting block 2057 is fixedly connected to the sampling dial hand horizontal motion slider connecting block 2053; the sampling dial hand horizontal belt 2058 is sleeved on the sampling dial hand motion driving wheel 2059 and the sampling dial hand motion driven wheel 20510, the sampling dial hand driving wheel 2059 is connected to the sampling dial hand horizontal motor 2056, and the sampling dial hand horizontal motion The movable slider connecting block 2053 is sleeved on the horizontal belt 2058 of the sampling dial hand; the sampling dial hand horizontal motor 2056 is started, and the chip sampling dial hand 203 moves along the sampling dial hand horizontal slide rail 2052. The initial position of the chip sampling dial hand 203 is above the front end of the chip sampling slot 204 (the high starting point of the sampling dial hand slide slot). After the chip is placed in, the chip insertion sensor 2033 senses the chip, and the chip sampling dial hand 203 moves along the sampling dial hand slide slot 2032, and the front end of the sampling dial hand slide slot 2032 is set to be higher than the rear end, so as not to block the chip from being placed in the chip sampling slot 204. The chip sampling dial hand 203 moves the chip so that the chip completely reaches the preset position of the chip sampling slot 204 to prevent the chip from being placed in place.

[0094] like Figures 13-15As shown, the liquid-gas circuit module 202 includes a liquid-gas circuit control motor 2021 and a chip valve control motor 2022; the liquid-gas circuit control motor 2021 drives the annular bracket 2023 to move downward, and the annular bracket 2023 is pressed downward so that the injection port interface 20224 located on the annular bracket 2023 is sealed to the injection chamber of the docking chip through the injection port sealing head 20225, and the external gas-liquid port interface 2028 is sealed to the external gas-liquid port of the docking chip through the external gas-liquid port sealing joint 2026; a liquid detection rod 2024 is fixedly connected to the annular bracket 2023, and the liquid detection rod 2024 is connected to the liquid detection sensor 2025; the chip valve control motor 2022 drives the chip valve slider connecting block 20218 to move, and the chip valve slider connecting block 20218 is installed with a chip valve pressure rod 2027, and the chip valve pressure rod 2027 is pressed down to press the chip valve under the control of the chip valve control motor 2022.

[0095] like Fig.14 As shown, the liquid-gas circuit module 202 also includes a liquid-gas circuit rail 2029 and a liquid-gas circuit slider 20210; the liquid-gas circuit control motor 2021 is started, the liquid-gas circuit screw rod 20211 rotates, and the liquid-gas circuit motor nut 20212 on the liquid-gas circuit screw rod 20211 drives the annular bracket 2023 to move downward, and the annular bracket 2023 is connected to the liquid-gas circuit rail 2029, and moves in coordination with the liquid-gas circuit slider 20210, and the liquid-gas circuit slider 20210 is fixedly connected to the inner side of the side bracket 20213 of the liquid-gas circuit module 202.

[0096] like Fig.15As shown, the liquid circuit and gas circuit module 202 also includes a chip valve slide rail 20214 and a chip valve slider 20215; the chip valve control motor 2022 is started, the chip valve screw rod 20216 rotates, and the chip valve motor nut 20217 on the chip valve screw rod 20216 drives the chip valve slider connecting block 20218 to move, and the chip valve slider connecting block 20218 is connected to the chip valve slider 20215, and the chip valve slide rail 20214 is installed on the inner side of the support plate 20219 of the liquid circuit and gas circuit module 202. The liquid and gas circuit control motor 2021 is started, the liquid and gas circuit screw rod 20211 rotates, and the liquid and gas circuit motor nut 20212 drives the annular bracket 2023 to move downward. The annular bracket 2023 is connected to the liquid and gas circuit slide rail 2029, and moves in coordination with the liquid and gas circuit slider 20210. The liquid and gas circuit slider 20210 is fixedly connected to the inner side of the side bracket 20213. The annular bracket 2023 is pressed down to make the injection port interface 20224 (air intake pressurization effect) located on the annular bracket 2023 seal the injection cavity of the docking chip through the injection port sealing head 20225, and the external gas-liquid port interface 2028 (sealed treatment, gas and liquid cannot enter) is sealed through the external gas-liquid port sealing joint 2026. Then, the air intake pressurization through the injection port interface 20224 makes the sample in the chip injection cavity slowly move forward along the chip microchannel. A liquid detection rod 2024 is fixedly connected to the frame 2023, and the liquid detection rod 2024 is connected to the liquid detection sensor 2025. When the sample in the chip reaches the chip conductive rubber valve, the liquid detection rod senses the capacitance change, the chip valve control motor 2022 of the instrument is started, the chip valve screw rod 20216 rotates, and the chip valve motor nut 20217 drives the chip valve slider connecting block 20218 to move. The chip valve slider connecting block 20218 is connected to the chip valve slider 20215, the chip valve slide rail 20214 is installed on the inner side of the support plate 20219, and the chip valve pressure rod 2027 is installed on the chip valve slider connecting block 20218. The chip valve pressure rod 2027 presses down the valve under the control of the chip valve control motor 2022, so that the chip liquid cannot continue to move forward, and at the same time, the injection port interface 20224 stops the air intake pressurization.

[0097] like Fig.13 As shown, the liquid circuit and gas circuit module 202 is also provided with a chip valve limiting optical coupler 20220 and a chip valve limiting baffle 20221, which are used to limit the descending height of the chip valve pressure rod 2027; the liquid circuit and gas circuit module 202 is also provided with a liquid circuit and gas circuit limiting optical coupler 20223 and a liquid circuit and gas circuit limiting baffle 20222, which are used to limit the descending height of the annular bracket 2023.

[0098] The liquid and gas path module 202 also includes a chip scanner 207 for scanning the QR code on the chip. The chip scanner 207 is located above the chip and scans the chip through the through hole 2081 on the top plate 208 of the injection module 2 to confirm whether the chip detection items are correct and whether the chip is within the validity period. If it does not meet the requirements, the machine alarms and prompts an error message.

[0099] like Figures 16-20 As shown, the incubation module 3 has an incubation tank component 301, such as Fig.17 As shown, the incubation tank assembly 301 includes a heating plate 30101 and a heat preservation plate 30102; Fig.19 As shown, the incubation tank assembly 301 includes a plurality of stacked incubation tanks 30103, and the stacked incubation tanks 30103 can freely move up and down along the incubation tank slide rail 302. The incubation tank assembly 301 is provided with a heating plate 30101 and a heat preservation plate 30102 to allow the chip to react at a constant temperature within a set temperature.

[0100] like Fig.16 As shown, the incubation trough assembly 301 is also provided with an incubation trough connecting block 303; the incubation trough connecting block 303 is connected to the incubation trough motion belt 305, and the brake motor 304 drives the incubation trough motion active wheel 306, and the incubation trough motion belt 305 drives the incubation trough motion driven wheel 307 to rotate, so that the multiple stacked incubation troughs 3013 in the incubation trough assembly 301 can move freely up and down along the incubation trough slide rail 302.

[0101] The incubation tank slide rail 302 is arranged on the incubation tank slide rail bracket 308, and a buffer block 309 is arranged on the side of the incubation tank assembly 301. Correspondingly, a buffer pad 3081 is arranged at the bottom of the incubation tank slide rail bracket 308; a limiting baffle 3010 is arranged on the incubation tank assembly 301, and the incubation tank slide rail bracket 308 is fixedly connected to a limiting optical coupler connecting block 3011. The limiting optical coupler 3012 is installed on the limiting optical coupler connecting block 3011, which is used to limit the up and down movement of the incubation tank 30103.

[0102] like Fig. 20 As shown, the incubation tank 30103 is provided with a chip pressure strip 3012, and the two protrusions of the chip pressure strip 3012 cooperate with the grooves in the chip and are fixed by nuts 3014 for positioning the chip for incubation reaction.

[0103] The heating plate 30101 and the heat preservation plate 30102 are both arranged on the side of the multiple stacked incubation tanks 30103.

[0104] like Figure 21-24As shown, the cleaning module 4 includes a U-shaped finger assembly and a cleaning module gas-liquid circuit assembly 402; after the chip incubation reaction is completed, the U-shaped finger 403 in the U-shaped finger assembly moves the chip to the cleaning position in the cleaning module gas-liquid circuit assembly 402 for cleaning; after the chip cleaning is completed, the U-shaped finger 403 moves the chip back; the cleaning module 4 also includes cleaning side brackets 401 on both sides.

[0105] like Fig. 22 As shown, the U-shaped dial hand assembly is provided with a U-shaped dial hand control motor 404. Under the control of the U-shaped dial hand control motor 404, the U-shaped dial hand driving wheel 405 rotates, and the U-shaped dial hand driven wheel 407 is driven by the U-shaped dial hand belt 406. The U-shaped dial hand belt connecting block 408 on the U-shaped dial hand belt 406 is connected to the U-shaped dial hand slider assembly 409, and the U-shaped dial hand slider assembly 409 is connected to the U-shaped dial hand 403 and moves along the U-shaped dial hand slide rail 4010.

[0106] The U-shaped finger 403 extends to two sides to form a U-shaped finger arm 1 4031 and a U-shaped finger arm 2 4032 , respectively. The distance between the U-shaped finger arm 1 4031 and the U-shaped finger arm 2 4032 is greater than the length of the chip.

[0107] The cleaning module gas-liquid circuit assembly 402 has a cleaning module control motor 4021. When the cleaning module control motor 4021 is started, the cleaning module screw 4022 rotates, and the cleaning module motor nut 4023 on the cleaning module screw 4022 drives the cleaning module annular bracket 4024 to move up and down; the cleaning module annular bracket 4024 is fixedly connected to the cleaning module slide rail 4025, and the cleaning module slide rail 4025 is slidably connected to the cleaning module slider 4026; the cleaning module annular bracket A cleaning module liquid detection rod 4027 and a cleaning module gas-liquid circuit interface 4028 are provided on 4024, and the cleaning module gas-liquid circuit interface 4028 are respectively a cleaning liquid interface 40281 and an external gas circuit interface 40282, and the cleaning liquid interface 40281 and the external gas circuit interface 40282 correspond to the cleaning module external gas-liquid port sealing joint 40213 at the bottom of the cleaning module annular bracket 4024; the cleaning module liquid detection rod 4027 is connected to the cleaning module liquid detection sensor 4029.

[0108] The cleaning module gas-liquid circuit assembly 402 is also provided with a cleaning module limit optical coupler 40210 and a cleaning module limit baffle 40211, which are used to limit the height of the cleaning module annular bracket 4024 to fall; the cleaning module annular bracket 4024 is also provided with a cleaning module chip injection port sealing joint 40212 at the bottom, and the cleaning module chip injection port sealing joint 40212 is located on the side of the cleaning module external gas-liquid port sealing joint 40213. When the cleaning module control motor 4021 is started, the cleaning module annular bracket 4024 can move up and down. When the chip is cleaned, the cleaning module annular bracket 4024 is pressed down, and the two sealing joints (the cleaning module external gas-liquid port sealing joint 40213 and the chip injection port sealing joint 40212) are respectively sealed and combined with the corresponding ports on the chip, and the cleaning liquid is added. When the cleaning module liquid detection rod 4027 detects the liquid, the liquid addition is stopped, and the external gas circuit is pressurized to make the cleaning liquid flow into the waste liquid chamber, and the cleaning is repeated 3-4 times. The cleaned chip is moved by the U-shaped handle 403 into the incubation tank 30103, the incubation tank 30103 rises to the corresponding position of the chip detection module 5, and is moved to the detection position by the detection handle 503. The detection position is located on the chip carrier 504. The chip carrier 504 is moved to enable the optical path detection component 501 to detect the chip.

[0109] like Figures 25 to 31 As shown, the chip detection module 5 includes an optical path detection component 501 and a detection hand assembly 502; the detection hand 503 in the detection hand assembly 502 moves the chip to the detection position, and the detection position is located on the chip carrier 504. The chip carrier 504 moves so that the optical path detection component 501 detects the chip; the chip detection module 5 is provided with an upper shielding cover 505 and a lower shielding cover 506. Since light protection is required during detection, the chip detection module 5 is provided with two shielding covers, the upper shielding cover 505 and the lower shielding cover 506, to reduce the influence of ambient light on the detection results.

[0110] The detection dial hand assembly 502 has a detection dial hand control motor 5021, and the detection dial hand control motor 5021 drives the detection dial hand control motor driving wheel 5022 to rotate. The detection dial hand control motor driving wheel 5022 drives the detection dial hand control motor driven wheel 5024 through the detection dial hand control motor belt 5023; the detection dial hand 503 is connected to the detection dial hand slider connecting block 5025, and the detection dial hand slider connecting block 5025 is fixedly connected with a detection dial hand slider 5026, and the detection dial hand slider 5026 and the detection dial hand 503 move along the detection dial hand slide rail 5027.

[0111] A detection hand limiting piece 5031 is provided at one end of the detection hand 503 , and a detection hand limiting optical coupler connecting block 5032 provided on the bottom plate 507 of the chip detection module 5 is fixedly connected to the detection hand limiting optical coupler 5033 for limiting the movement of the detection hand 503 .

[0112] like Fig.29 As shown, the optical path detection component 501 has a light head 5011, and a transmitting plate 5012 is arranged on the upper part of the light head 5011; a receiving plate 5013 is arranged at the lower part of the detection position, and the transmitting plate 5012 emits laser, which passes through the light head 5011 to perform fluorescence excitation on the reactants in the chip reaction cavity located at the detection position, and then is received by the receiving plate 5013, and the detection result is calculated after the fluorescence is collected and amplified.

[0113] like Fig.28 As shown, below the transmitting plate 5012, a first filter 5014, a first lens 5015, a second lens 5016 and a second filter 5017 are arranged in sequence from top to bottom; the receiving plate 5013 is arranged below the second filter 5017; wherein the first filter 5014 and the first lens 5015 are part of the daylighting head 5011; the daylighting head 5011 is connected to the daylighting head fixing block 50111, and the daylighting head fixing block 50111 is connected to the bottom plate 507 of the chip detection module 5.

[0114] A first light shielding block 5018 and a second light shielding block 5019 are respectively disposed on the sides of the second lens 5015 and the second filter 5017 ; the second light shielding block 5019 blocks the ambient light from the chip disposal channel 508 and serves as a connecting block of the chip disposal channel 508 .

[0115] like Fig.30 As shown, the chip carrier 504 is driven by a chip carrier control motor 5041, and the chip carrier control motor 5041 drives the chip carrier lead screw 5042 to rotate, and the chip carrier motor nut connecting block 5043 on the chip carrier lead screw 5042 moves; the chip carrier motor nut connecting block 5043 is connected to the chip carrier 504, and the chip carrier 504 is connected to a chip carrier slider 1 5044 and a chip carrier slider 2 5045; the chip carrier slider 1 5044 and the chip carrier slider 2 5045 drive the chip carrier 504 to move along the chip carrier slide rail 5046.

[0116] like Figures 32-33As shown, the chip detection module 5 is also provided with a discarding hand 509 for transferring the detected chip to the chip discarding channel 508; the discarding hand 509 is driven by a discarding hand control motor 5091, a discarding hand slider 5092 is fixed on the top plate 5010 of the chip detection module 5, and a discarding hand slide rail 5093 is connected to a discarding hand motor nut connecting block 5094; the discarding hand control motor 5091 drives the discarding hand screw 5095 to rotate, The discarding hand motor nut connecting block 5094 moves along the discarding hand screw rod 5095, and the discarding hand motor nut connecting block 5094 is connected to the vertical plate 5096, and the vertical plate 5096 is connected to the horizontal plate 5097; one end of the discarding hand 509 is connected to the horizontal plate 5097 as a discarding hand fixing part 50910, and the other end of the discarding hand 509 is used as a discarding hand moving part 5099 for moving the chip into the chip discarding channel 508.

[0117] A discarded dial limit block 5098 is fixed on the discarded dial motor nut connecting block 5094, and the discarded dial sensing part 50100 of the discarded dial limit block 5098 cooperates with the discarded dial limit sensor 50101 installed on the top plate 5010 to limit the discarded dial 509; two discarded dial hands 509 are provided, which are respectively arranged on both sides of the horizontal plate 5097.

[0118] When used specifically:

[0119] The sampling module 2 is specifically divided into a chip loading module 201 and a liquid and gas path module 202; first, the chip is inserted from the instrument sampling port 8, and the chip scanner 207 on the liquid and gas path module 202 scans the QR code of the chip to confirm whether the chip detection items are correct and whether the chip is within the validity period. If it does not meet the requirements, the machine will alarm and prompt an error message. At the same time, the chip insertion sensor 2033 senses the chip, and the chip sampling dial 203 slides to move the chip to put it into the chip sampling slot 204. A chip limiter structure 206 is set behind the chip sampling slot 204 to prevent the chip from being placed beyond the preset position of the chip sampling slot 204. Then the chip moves to the liquid and gas path module 202 driven by the chip injection slot 204, the liquid and gas path control motor 2021 is started, and the annular bracket 2023 is pressed down to make the injection port interface 20224 (air intake pressurization effect) located on the annular bracket 2023 seal the injection cavity of the docking chip, and the external gas-liquid port interface 2028 (sealed treatment, gas and liquid cannot enter) seals the external gas-liquid port of the docking chip, and then the air intake pressurization is carried out through the injection port connector to make the sample in the chip injection cavity slowly move forward along the chip microchannel. When it reaches the chip conductive rubber valve, the liquid detection rod 2024 senses the capacitance change, and the chip valve control motor 2022 of the instrument is started. The chip valve pressure rod 2027 presses down the valve under the control of the motor to prevent the chip liquid from moving forward.

[0120] The chip sample pusher 203 pushes the chip under the control of the motor so that the chip reaches the incubation module 3. The incubation module 3 has a heating plate 30101 and a heat preservation plate 30102, so that the chip reaction chamber performs sample immune reaction at a preset temperature.

[0121] After the reaction is completed, the U-shaped handle 403 of the cleaning module 4 moves the chip to the cleaning module 4. The cleaning module 4 has a cleaning module gas-liquid path component 402 that can clean the chip after the reaction. The cleaning module liquid detection rod 4027 on the cleaning module annular bracket 4024 can sense whether the liquid has reached the valve. After sensing the signal, the external liquid path and gas path port of the chip are pressurized to allow the cleaned liquid to enter the waste liquid cavity of the chip. After repeating this 3-4 times, the chip cleaning is completed, and then the U-shaped handle 403 moves the chip to the incubation module 3, and the incubation module 3 rises to the corresponding position of the chip detection module 5.

[0122] The detection handle 503 of the chip detection module 5 moves the chip to the chip carrier 504, and the chip carrier 504 can move so that the optical path detection component 501 performs optical signal detection on the chip reaction chamber. After the detection is completed, the disposal handle 509, under the control of the motor, moves the chip into the disposal tank 9 for biological waste treatment. The detection results can be displayed on the display screen 7 of the instrument, and can also be printed through the built-in printer 10.

[0123] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, such as making some adjustments to the positions of the various components mounted on the base. These changes and improvements fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A time-resolved fluorescence immunoassay analyzer, comprising a base, characterized in that: It also includes a sampling module, an incubation module, a cleaning module and a chip detection module; The sample injection module is provided with a chip loading module and a liquid and gas path module, wherein the chip loading module is used to carry the chip to the liquid and gas path module, and the liquid and gas path module is used to make the sample in the chip flow to a predetermined position; and then the chip is transported to the incubation module; The incubation module allows the chip to react at a constant temperature within a set temperature; The incubation module freely moves up and down in the vertical direction, and the injection module, the cleaning module and the chip detection module are respectively arranged on the side of the vertical movement direction of the incubation module; the incubation module sends the chip cleaned in the cleaning module to the chip detection module for detection; The cleaning module includes a U-shaped hand assembly and a cleaning module gas-liquid circuit assembly; after the chip incubation reaction is completed, the U-shaped hand in the U-shaped hand assembly moves the chip to the cleaning position in the cleaning module gas-liquid circuit assembly for cleaning; after the chip cleaning is completed, the U-shaped hand moves the chip back; The chip detection module includes an optical path detection component and a detection handle component; the detection handle in the detection handle component moves the chip to the detection position, the detection position is located on the chip carrier, and the chip carrier moves so that the optical path detection component detects the chip; the chip detection module is provided with an upper shielding cover and a lower shielding cover.

2. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The incubation module comprises an incubation tank assembly, which comprises a heating plate and a heat preservation plate; the incubation tank assembly comprises a plurality of stacked incubation tanks, and the plurality of stacked incubation tanks freely move up and down along the incubation tank slide rails.

3. The time-resolved fluorescence immunoassay analyzer according to claim 2, characterized in that: The incubation trough assembly is also provided with an incubation trough connecting block; the incubation trough connecting block is connected to the incubation trough motion belt, the brake motor drives the incubation trough motion active wheel, and the incubation trough motion belt drives the incubation trough motion driven wheel to rotate, so that the multiple stacked incubation troughs in the incubation trough assembly can move freely up and down along the incubation trough slide rail.

4. The time-resolved fluorescence immunoassay analyzer according to claim 3, characterized in that: The incubation trough slide rail is arranged on the incubation trough slide rail bracket, and a buffer block is arranged on the side of the incubation trough assembly. Correspondingly, a buffer pad is arranged at the bottom of the incubation trough slide rail bracket; a limit baffle is arranged on the incubation trough assembly, and the incubation trough slide rail bracket is fixedly connected to a limit optical coupler connecting block. The limit optical coupler is installed on the limit optical coupler connecting block and is used to limit the up and down movement of the incubation trough.

5. The time-resolved fluorescence immunoassay analyzer according to claim 4, characterized in that: The incubation tank is provided with a chip pressure strip, and two protrusions of the chip pressure strip cooperate with the grooves of the chip to position the chip for incubation reaction.

6. The time-resolved fluorescence immunoassay analyzer according to claim 5, characterized in that: The heating plate and the heat-insulating plate are both arranged on the side surfaces of the plurality of stacked incubation tanks.

7. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The chip loading module includes a chip sampling lever, which moves the chip to put it into place in the chip sampling slot; a chip limiting piece structure is arranged behind the chip sampling slot; the chip moves driven by the chip sampling slot.

8. The time-resolved fluorescence immunoassay analyzer according to claim 7, characterized in that: The chip sampling dial hand is controlled by a chip sampling dial hand motion component; the chip sampling dial hand motion component includes a sampling dial hand horizontal motion component and a sampling dial hand up and down motion component; the sampling dial hand horizontal motion component has a sampling dial hand horizontal motion slider, the sampling dial hand horizontal motion slider moves along the sampling dial hand horizontal slide rail, the sampling dial hand horizontal motion slider is connected to the sampling dial hand horizontal motion slider connecting block, the sampling dial hand horizontal motion slider connecting block is fixedly connected with the chip sampling dial hand up and down motion slide rail, the sampling dial hand up and down motion slider is connected to the chip sampling dial hand up and down motion slide rail, the sampling dial hand up and down motion slider is connected to the chip sampling dial hand, and the chip sampling dial hand is provided with a chip sampling dial hand slide rod, and the chip sampling dial hand slide rod moves in the chip sampling dial hand slide groove.

9. The time-resolved fluorescence immunoassay analyzer according to claim 8, characterized in that: The chip sampling slot is controlled by the sampling slot motion motor, and the sampling slot motor nut moves along the sampling slot lead screw, the sampling slot motor nut is connected to the sampling slot connecting block 1, the sampling slot connecting block 1 is connected to the chip sampling slot, the sampling slot connecting block 2 is connected to the sampling slot connecting block 1, the sampling slot connecting block 2 is connected to the sampling slot slider, and the sampling slot slider reciprocates along the sampling slot slide rail.

10. The time-resolved fluorescence immunoassay analyzer according to claim 9, characterized in that: There are two injection slot slides on the partition of the chip loading module, namely injection slot slide 1 and injection slot slide 2; correspondingly, there are two injection slot sliders, namely injection slot slider 1 and injection slot slider 2, and there are two injection slot connecting blocks 2, namely first injection slot connecting block 2 and second injection slot connecting block 2; wherein, the first injection slot connecting block 2 is connected to the injection slot slider 1, and the injection slot slider 1 reciprocates along the injection slot slide 1, and the second injection slot connecting block 2 is connected to the injection slot slider 2, and the injection slot slider 2 reciprocates along the injection slot slide 2.

11. The time-resolved fluorescence immunoassay analyzer according to claim 9, characterized in that: The partition is provided with a chip loading module bracket one and a chip loading module bracket two, wherein the chip loading module bracket two is provided with a chip limiting plate slide groove; the chip limiting plate structure has a chip limiting plate, and the chip limiting plate is provided with a chip limiting plate slide rod for moving in the chip limiting plate slide groove; a chip limiting plate slide rail is fixedly connected to the side wall of the chip feeding slot, and a chip limiting plate slider is connected to the chip limiting plate slide rail, and the chip limiting plate is connected to the chip limiting plate slider, and the chip limiting plate follows the horizontal movement of the chip feeding slot and cooperates with the chip limiting plate slide groove to move up and down.

12. The time-resolved fluorescence immunoassay analyzer according to claim 8, characterized in that: The front end of the sampling dial hand slide is higher than the rear end, so that the sampling dial hand slide has a high starting point; the sampling dial hand horizontal motion component has a sampling dial hand horizontal motor, one end of the sampling dial hand belt connecting block is fixedly connected to the sampling dial hand horizontal belt, and the other end of the sampling dial hand belt connecting block is fixedly connected to the sampling dial hand horizontal motion slider connecting block.

13. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The liquid-gas circuit module includes a liquid-gas circuit control motor and a chip valve control motor; the liquid-gas circuit control motor drives the annular bracket to move downward, and the annular bracket is pressed downward so that the injection port interface located on the annular bracket is sealed to the injection chamber of the docking chip through the injection port sealing head, and the external gas-liquid port interface is sealed to the external gas-liquid port of the docking chip through the external gas-liquid port sealing joint; a liquid detection rod is fixedly connected to the annular bracket, and the liquid detection rod is connected to the liquid detection sensor; the chip valve control motor drives the chip valve slider connecting block to move, and a chip valve pressure rod is installed on the chip valve slider connecting block, and the chip valve pressure rod presses down the chip valve under the control of the chip valve control motor.

14. The time-resolved fluorescence immunoassay analyzer according to claim 13, characterized in that: The liquid and gas circuit module also includes a liquid and gas circuit rail and a liquid and gas circuit slider; the liquid and gas circuit control motor is started, the liquid and gas circuit screw rod rotates, and the liquid and gas circuit motor nut on the liquid and gas circuit screw rod drives the annular bracket to move downward, and the annular bracket is connected to the liquid and gas circuit rail and moves in coordination with the liquid and gas circuit slider, and the liquid and gas circuit slider is fixedly connected to the inner side of the side bracket of the liquid and gas circuit module.

15. The time-resolved fluorescence immunoassay analyzer according to claim 14, characterized in that: The liquid and gas circuit module also includes a chip valve slide rail and a chip valve slider; the chip valve control motor is started, the chip valve screw rod rotates, the chip valve motor nut on the chip valve screw rod drives the chip valve slider connecting block to move, the chip valve slider connecting block is connected to the chip valve slider, and the chip valve slide rail is installed on the inner side of the support plate of the liquid and gas circuit module.

16. The time-resolved fluorescence immunoassay analyzer according to claim 15, characterized in that: The liquid circuit and gas circuit module is also provided with a chip valve limiting optical coupler and a chip valve limiting baffle, which are used to limit the height of the chip valve pressure rod descending; the liquid circuit and gas circuit module is also provided with a liquid circuit and gas circuit limiting optical coupler and a liquid circuit and gas circuit limiting baffle, which are used to limit the height of the annular bracket descending.

17. The time-resolved fluorescence immunoassay analyzer according to claim 16, characterized in that: The liquid and gas path module also includes a chip scanner for scanning the two-dimensional code on the chip.

18. The time-resolved fluorescence immunoassay analyzer according to claim 2, characterized in that: The U-shaped dial hand assembly is provided with a U-shaped dial hand control motor. Under the control of the U-shaped dial hand control motor, the U-shaped dial hand driving wheel rotates, and the U-shaped dial hand driven wheel is driven by the U-shaped dial hand belt. The U-shaped dial hand belt connecting block on the U-shaped dial hand belt is connected to the U-shaped dial hand slider assembly, and the U-shaped dial hand slider assembly is connected to the U-shaped dial hand and moves along the U-shaped dial hand slide rail.

19. The time-resolved fluorescence immunoassay analyzer according to claim 18, characterized in that: The U-shaped push arm extends to two sides to form a U-shaped push arm 1 and a U-shaped push arm 2, respectively. The distance between the U-shaped push arm 1 and the U-shaped push arm 2 is greater than the length of the chip.

20. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The cleaning module gas-liquid circuit assembly comprises a cleaning module control motor, when the cleaning module control motor starts, the cleaning module lead screw rotates, and the cleaning module motor nut on the cleaning module lead screw drives the cleaning module annular bracket to move up and down; the cleaning module annular bracket is fixedly connected to the cleaning module slide rail, and the cleaning module slide rail is slidably connected to the cleaning module slider; the cleaning module annular bracket is provided with a cleaning module liquid detection rod and a cleaning module gas-liquid circuit interface, and the cleaning module gas-liquid circuit interfaces are cleaning liquid interfaces and external gas circuit interfaces respectively, and the cleaning liquid interfaces and external gas circuit interfaces correspond to the cleaning external gas-liquid port sealing joint at the bottom of the cleaning module annular bracket; the cleaning module liquid detection rod is connected to the cleaning module liquid detection sensor.

21. The time-resolved fluorescence immunoassay analyzer according to claim 20, characterized in that: The cleaning module gas-liquid circuit assembly is also provided with a cleaning module limiting optical coupler and a cleaning module limiting baffle, which are used to limit the height of the cleaning module annular bracket. A cleaning module chip inlet sealing joint is also provided at the bottom of the cleaning module annular bracket, and the cleaning module chip inlet sealing joint is located on the side of the cleaning module external gas-liquid port sealing joint.

22. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The detection dial hand assembly comprises a detection dial hand control motor, the detection dial hand control motor drives the detection dial hand control motor driving wheel to rotate, and the detection dial hand control motor driving wheel drives the detection dial hand control motor driven wheel through the detection dial hand control motor belt; the detection dial hand is connected to a detection dial hand slider connecting block, the detection dial hand slider connecting block is fixedly connected with a detection dial hand slider, and the detection dial hand slider and the detection dial hand move along the detection dial hand sliding rail.

23. The time-resolved fluorescence immunoassay analyzer according to claim 22, characterized in that: A detection dial hand limiting piece is arranged at one end of the detection dial hand, and a detection dial hand limiting optical coupler connecting block arranged on the bottom plate of the chip detection module is fixedly connected to the detection dial hand limiting optical coupler for limiting the movement of the detection dial hand.

24. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The optical path detection component has a light-collecting head, and a transmitting plate is arranged at the upper part of the light-collecting head; a receiving plate is arranged at the lower part of the detection position. The transmitting plate emits laser, which passes through the light-collecting head to perform fluorescence excitation on the reactants in the chip reaction cavity at the detection position, and then is received by the receiving plate. The detection result is calculated after the fluorescence is collected and amplified.

25. The time-resolved fluorescence immunoassay analyzer according to claim 24, characterized in that: Below the transmitting board, a first filter, a first lens, a second lens and a second filter are arranged in sequence from top to bottom; the receiving board is arranged below the second filter; wherein the first filter and the first lens are part of the daylighting head; the daylighting head is connected to a daylighting head fixing block, and the daylighting head fixing block is connected to the bottom plate of the chip detection module.

26. The time-resolved fluorescence immunoassay analyzer according to claim 25, characterized in that: A first light shielding block and a second light shielding block are respectively arranged on the sides of the second lens and the second filter; the second light shielding block shields the ambient light from the chip disposal channel and serves as a connecting block of the chip disposal channel.

27. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The chip carrier is driven by a chip carrier control motor, and the chip carrier control motor drives the chip carrier lead screw to rotate, and the chip carrier motor nut connecting block on the chip carrier lead screw moves; the chip carrier motor nut connecting block is connected to the chip carrier, and the chip carrier is connected to a chip carrier slider 1 and a chip carrier slider 2; the chip carrier slider 1 and the chip carrier slider 2 drive the chip carrier to move along the chip carrier slide rail.

28. The time-resolved fluorescence immunoassay analyzer according to claim 1, characterized in that: The chip detection module is also provided with a discarding hand, which is used to transfer the detected chip to the chip discarding channel; the discarding hand is driven by a discarding hand control motor, the discarding hand slider is fixed on the top plate of the chip detection module, and the discarding hand slide rail is connected to the discarding hand motor nut connecting block; The disposal dial control motor drives the disposal dial lead screw to rotate, and the disposal dial motor nut connecting block moves along the disposal dial lead screw, and the disposal dial motor nut connecting block is connected to the vertical plate, and the vertical plate is connected to the horizontal plate; one end of the disposal dial is connected to the horizontal plate as a disposal dial fixing part, and the other end of the disposal dial is used as a disposal dial toggling part for toggling the chip into the chip disposal channel.

29. The time-resolved fluorescence immunoassay analyzer according to claim 28, characterized in that: A discarded dial hand limit block is fixed on the discarded dial hand motor nut connecting block, and a discarded dial hand sensing portion of the discarded dial hand limit block cooperates with a discarded dial hand limit sensor installed on the top plate to limit the discarded dial hand.

30. The time-resolved fluorescence immunoassay analyzer according to claim 29, characterized in that: The disposal levers are provided with two, which are respectively arranged on both sides of the horizontal plate.

31. The time-resolved fluorescence immunoassay analyzer according to any one of claims 1 to 30, characterized in that: The exterior of the time-resolved fluorescence immunoassay analyzer is also covered with a shell, on which a display screen is arranged; the shell is provided with an injection port for inserting a chip; a disposal groove is also provided on the side of the shell; the time-resolved fluorescence immunoassay analyzer is also provided with a built-in printer, and a paper outlet of the built-in printer is arranged on the shell.

Images