Medicinal and edible plant composition and application thereof

Abstract

The invention relates to a medicinal and edible plant composition and application thereof. The medicinal and edible plant composition is an aqueous extract of the following preparation raw materials: kudzuvine root, hovenia dulcis thunb, chrysanthemum bud, poria cocos and liquorice. According to the invention, five specific medicinal and edible raw materials are reasonably combined to prepare a water extraction product, the product has an excellent anti-oxidation effect, has an activation effect on ethanol dehydrogenase and acetaldehyde dehydrogenase, and has very good potential of dispelling the effects of alcohol and protecting the liver, and all the components are mutually matched and have a synergistic effect on the effects.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine, and relates to a medicinal and edible plant composition and application thereof. Background Art

[0002] Studies have shown that after alcohol enters the human body, about 90% of it enters the liver for metabolism, and a small amount is absorbed through gastrointestinal digestion. Excessive drinking can cause liver damage, alcoholic hepatitis, liver fibrosis, and even cirrhosis, liver failure, cancer and other diseases. The main pathway of alcohol metabolism is the metabolic pathway involving alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). The main metabolic process is that alcohol is metabolized into acetaldehyde under the action of ADH, and then metabolized into acetic acid under the action of ALDH, and finally metabolized into CO2, water and energy through the tricarboxylic acid cycle.

[0003] As consumers' health awareness continues to increase, the demand for alcohol sobering and liver protecting products continues to increase. The current liver protecting ingredients or products include milk thistle, dandelion, N-acetylcysteine, curcumin, enzyme preparations, etc. my country is rich in medicinal and edible resources, and many medicinal and edible materials can give products the effect of alcohol sobering and liver protecting. It is very meaningful to develop more medicinal and edible products that can effectively sober up and protect the liver. Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a medicinal and edible plant composition and its application.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a plant composition having both medicinal and edible properties, wherein the plant composition having both medicinal and edible properties is a water extract product prepared from the following raw materials: kudzu root, Hovenia dulcis, Chrysanthemum morifolium, Poria cocos and liquorice.

[0007] Pueraria root, Hovenia dulcis, Chrysanthemum officinale, Poria cocos, and Licorice are all food and medicine dual-purpose raw materials announced by the National Health Commission. Among them, Pueraria root is sweet, pungent, and cool, and it enters the spleen, stomach, and lung meridians. It can relieve muscles and reduce fever, promote fluid production and quench thirst, promote rash, raise yang and stop diarrhea, and promote blood circulation. Its main functional ingredients are puerarin, flavonoid glycosides, and proteins. Among them, Hovenia dulcis is sweet, neutral in nature, and enters the stomach meridian. It can quench thirst, relieve restlessness, stop vomiting, and promote urination. Its main functional ingredients are flavonoids, polysaccharides, Vc, and organic acids. Chrysanthemum, sweet, bitter, slightly cold, dispels wind and heat, soothes the liver and improves eyesight, clears away heat and detoxifies, and its main functional ingredients are volatile oils, flavonoids, terpenes, etc.; among them, Poria, sweet, light, flat, promotes diuresis and eliminates dampness, strengthens the spleen and calms the mind, and its main functional ingredients are Poria polysaccharide, triterpenoid compounds, Poria acid, etc.; among them, Licorice, sweet, flat, tonifies the spleen and replenishes Qi, clears away heat and detoxifies, eliminates phlegm and relieves cough, relieves acute pain, and harmonizes various medicines, and its main functional ingredients are glycyrrhizin, flavonoids, glycyrrhizic acid, etc.

[0008] The present invention reasonably combines the above five specific medicinal and edible raw materials to obtain a water extract product thereof. The composition has excellent antioxidant effect, has an activation effect on alcohol dehydrogenase and acetaldehyde dehydrogenase, has good alcohol sobering and liver protection potential, and each component cooperates with each other to synergistically enhance the above effects. The composition is widely used and can be used in food, health products, medicines, cosmetics, daily chemical products, etc.

[0009] Preferably, the raw materials for preparing the edible and medicinal plant composition are, by weight, 1-4 parts of Pueraria root, 0.5-2 parts of Hovenia dulcis, 0.5-2 parts of Chrysanthemum morifolium, 0.5-1.5 parts of Poria cocos and 0.5-1.5 parts of Licorice.

[0010] Based on the potential synergistic relationship between the five components, when they are combined in the above-mentioned specific mass ratio, their effects in anti-oxidation and activating alcohol dehydrogenase and acetaldehyde dehydrogenase are more significant.

[0011] The mass fraction of the kudzu root can be selected as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, etc.; the mass fraction of the Hovenia dulcis can be selected as 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, etc.; the mass fraction of the chrysanthemum can be selected as 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, etc.; the mass fraction of the Poria can be selected as 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, etc.; the mass fraction of the liquorice can be selected as 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, etc. Other specific point values ​​within the above numerical ranges can be selected, and they will not be repeated here one by one.

[0012] The present invention also found that when the medicinal and edible plant composition is prepared by the following specific method, that is, the five raw materials are divided into two groups and respectively extracted by ultrasonic extraction combined with reflux extraction, the functional components can be dissolved as much as possible, and the antioxidant effect and the effect of activating alcohol dehydrogenase and acetaldehyde dehydrogenase are more significant, and the color and tissue state of the product are more excellent. In addition, the preparation method is free of organic solvents throughout the process, is green and environmentally friendly, has low energy consumption, high efficiency, simple operation, and is suitable for continuous large-scale production.

[0013] Preferably, the medicinal and edible plant composition is prepared by a preparation method comprising the following steps:

[0014] (1) mixing the crushed Pueraria root, Hovenia dulcis and Chrysanthemum officinale with water and performing ultrasonic extraction, filtering the extract to obtain a filter residue and a first filtrate; mixing the filter residue with water and performing reflux extraction, filtering the extract to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0015] (2) mixing the crushed Poria cocos and licorice with water and performing ultrasonic extraction, filtering the extract to obtain a filter residue and a third filtrate; mixing the filter residue with water and performing reflux extraction, filtering the extract to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product;

[0016] (3) combining the first product and the second product to obtain the medicinal and edible plant composition;

[0017] There is no distinction between the order of steps (1) and (2).

[0018] Preferably, the pulverizing in step (1) and step (2) means that each group is independently pulverized to 40-100 mesh, such as 40 mesh, 50 mesh, 60 mesh, 70 mesh, 80 mesh, 100 mesh, etc.

[0019] Preferably, the material-liquid ratio of the plant raw material to water in step (1) and step (2) is independently 1:(8-18) g / mL, for example 1:8 g / mL, 1:10 g / mL, 1:12 g / mL, 1:14 g / mL, 1:15 g / mL, 1:16 g / mL, 1:18 g / mL, etc.

[0020] Preferably, the power of the ultrasonic extraction in step (1) and step (2) is each independently 100-300 W, for example 100 W, 150 W, 200 W, 250 W, 300 W, etc.; the temperature of the ultrasonic extraction is each independently 40-55 ° C, for example 40 ° C, 42 ° C, 45 ° C, 48 ° C, 50 ° C, 55 ° C, etc.; the time of the ultrasonic extraction is each independently 20-40 min, for example 20 min, 25 min, 28 min, 30 min, 32 min, 35 min, 40 min, etc.

[0021] Preferably, the material-liquid ratio of the filter residue to water in step (1) and step (2) is independently 1:(8-18) g / mL, for example, 1:8 g / mL, 1:10 g / mL, 1:12 g / mL, 1:14 g / mL, 1:15 g / mL, 1:16 g / mL, 1:18 g / mL, etc.

[0022] Preferably, the temperature of the reflux extraction in step (1) and step (2) is independently 80-100°C, and the time of the reflux extraction is independently 20-60min, for example, 20min, 25min, 28min, 30min, 32min, 35min, 40min, 50min, 60min, etc.

[0023] Preferably, after obtaining the first filtrate and the second filtrate in step (1), the filtrate is allowed to stand at low temperature and centrifuged and then combined to obtain the first product; or after obtaining the first product in step (1), the filtrate is allowed to stand at low temperature and centrifuged;

[0024] And / or, the third filtrate and the fourth filtrate obtained in step (2) are allowed to stand at low temperature and centrifugally filtered before being combined to obtain the second product; or the second product obtained in step (1) is allowed to stand at low temperature and centrifugally filtered.

[0025] Preferably, the low-temperature standing is carried out at 2-10°C (e.g., 2°C, 4°C, 5°C, 6°C, 7°C, 8°C, 10°C, etc.) for 4-12h (e.g., 4h, 6h, 8h, 10h, 11h, 12h, etc.).

[0026] Preferably, the centrifugation is performed at a speed of 7000-10000 r / min (e.g. 7000 r / min, 8000 r / min, 9000 r / min, 10000 r / min, etc.) for 3-8 min (e.g. 3 min, 4 min, 5 min, 6 min, 7 min, 8 min, etc.).

[0027] Preferably, the medicinal and edible plant composition is also sterilized during the preparation process.

[0028] Preferably, the sterilization treatment conditions are: sterilization at 60-80°C (e.g., 60°C, 65°C, 70°C, 75°C, 80°C, etc.) for 20-40 min (e.g., 20 min, 25 min, 30 min, 35 min, 40 min, etc.).

[0029] Preferably, in step (3), the first product and the second product are combined and then concentrated and dried.

[0030] In a second aspect, the present invention provides a use of the medicinal and edible plant composition according to the first aspect in preparing a product with antioxidant efficacy.

[0031] Preferably, the products include food, health products, medicines, cosmetics, daily chemical products, etc.

[0032] In a third aspect, the present invention provides use of the edible and medicinal plant composition according to the first aspect in the preparation of an alcohol dehydrogenase activator and / or an acetaldehyde dehydrogenase activator.

[0033] In a fourth aspect, the present invention provides use of the medicinal and edible plant composition according to the first aspect in preparing a product for sobering up and protecting the liver.

[0034] Preferably, the products include food, health products, medicines, cosmetics, daily chemical products, etc.

[0035] In a fifth aspect, the present invention provides a plant beverage, the components of which include the medicinal and edible plant composition described in the first aspect and auxiliary additives.

[0036] Preferably, the auxiliary additive comprises any one of inulin, L-arabinose or fruit juice, or a combination of at least two thereof.

[0037] Preferably, the fruit juice comprises lemon juice, orange juice, apple juice, pear juice, tomato juice.

[0038] Preferably, the plant beverage is prepared by mixing and homogenizing the medicinal and edible plant composition and auxiliary additives.

[0039] The plant beverage prepared based on the above-mentioned medicinal and edible composition also has the potential for anti-oxidation, activation of alcohol dehydrogenase and acetaldehyde dehydrogenase, and hangover and liver protection. At the same time, the plant beverage is suitable for current consumers' consumption needs such as low sugar, low calories, and fruity flavor, and has less burden on the body.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] The present invention reasonably combines the above five specific medicinal and edible raw materials to obtain a water extract product thereof. The composition has excellent antioxidant effect, has an activation effect on alcohol dehydrogenase and acetaldehyde dehydrogenase, has good alcohol sobering and liver protection potential, and each component cooperates with each other to synergistically enhance the above effects. The composition is widely used and can be used in food, health products, medicines, cosmetics, daily chemical products, etc.

[0042] When the medicinal and edible plant composition of the present invention is prepared by a specific method, that is, five raw materials are divided into two groups and ultrasonic extraction combined with reflux extraction is performed respectively, so as to ensure that the functional components are dissolved as much as possible, and the antioxidant effect and the effect of activating alcohol dehydrogenase and acetaldehyde dehydrogenase are more significant, and the color and tissue state of the product are more excellent. In addition, the preparation method is free of organic solvents throughout the process, is green and environmentally friendly, has low energy consumption, high efficiency, simple operation, and is suitable for continuous large-scale production. DETAILED DESCRIPTION

[0043] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0044] Example 1

[0045] The present embodiment provides a medicinal and edible plant composition, the preparation raw materials of which include: 2 parts of Pueraria root, 1 part of Hovenia dulcis, 1 part of Chrysanthemum morifolium, 1 part of Poria cocos and 1 part of Licorice.

[0046] Its preparation method is:

[0047] (1) Mixing kudzu root, Hovenia dulcis and chrysanthemum root crushed to 70 mesh with water at a ratio of 1:12 g / mL and performing ultrasonic extraction, wherein the power of the ultrasonic extraction is 200 W, the temperature is 50° C. and the time is 30 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and performing reflux extraction, wherein the temperature of the reflux extraction is 90° C. and the time is 40 min, filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0048] (2) Mixing the Poria cocos and licorice powdered to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting the mixture to ultrasonic extraction at a power of 200 W, a temperature of 50° C., and a time of 30 min; filtering the extract to obtain a filter residue and a third filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting the mixture to reflux extraction at a temperature of 90° C. and a time of 40 min; filtering the extract to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product;

[0049] (3) The first product and the second product are respectively placed at 4° C. for 10 hours, centrifuged and filtered, and the centrifugation speed is 8000 r / min for 5 minutes. The products are then combined, sterilized at 70° C. for 25 minutes, and freeze-dried to obtain the medicinal and edible plant composition.

[0050] Example 2

[0051] The present embodiment provides a medicinal and edible plant composition, the raw materials for preparing the composition include: 4 parts of Pueraria root, 0.5 parts of Hovenia dulcis, 2 parts of Chrysanthemum morifolium, 0.5 parts of Poria cocos and 1.5 parts of Licorice.

[0052] Its preparation method is:

[0053] (1) Mixing kudzu root, Hovenia dulcis and chrysanthemum root crushed to 50 mesh with water at a ratio of 1:15 g / mL and performing ultrasonic extraction, wherein the power of the ultrasonic extraction is 300 W, the temperature is 55° C. and the time is 20 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:15 g / mL and performing reflux extraction, wherein the temperature of the reflux extraction is 100° C. and the time is 20 min, filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0054] (2) mixing the Poria cocos and licorice powdered to 50 mesh with water at a ratio of 1:15 g / mL and then subjecting the mixture to ultrasonic extraction at a power of 300 W, a temperature of 55° C., and a time of 20 min; filtering the extract to obtain a filter residue and a third filtrate; mixing the filter residue with water at a ratio of 1:15 g / mL and subjecting the mixture to reflux extraction at a temperature of 100° C. and a time of 20 min; filtering the extract to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product;

[0055] (3) The first product and the second product are respectively placed at 6° C. for 12 h, centrifuged and filtered, and the centrifugation speed is 7000 r / min for 8 min. The products are then combined, sterilized at 70° C. for 25 min, and freeze-dried to obtain the medicinal and edible plant composition.

[0056] Example 3

[0057] The present embodiment provides a medicinal and edible plant composition, the raw materials for preparing the composition include: 1 part of Pueraria root, 2 parts of Hovenia dulcis, 0.5 parts of Chrysanthemum morifolium, 1.5 parts of Poria cocos and 0.5 parts of Licorice.

[0058] Its preparation method is:

[0059] (1) Mixing kudzu root, Hovenia dulcis and chrysanthemum root crushed to 40 mesh with water at a ratio of 1:8 g / mL and performing ultrasonic extraction, wherein the power of the ultrasonic extraction is 100 W, the temperature is 45° C. and the time is 40 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:8 g / mL and performing reflux extraction, wherein the temperature of the reflux extraction is 80° C. and the time is 60 min, filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0060] (2) Mixing the Poria cocos and licorice powdered to 40 mesh with water at a ratio of 1:8 g / mL and then subjecting the mixture to ultrasonic extraction at a power of 100 W, a temperature of 45° C., and a time of 40 min; filtering the extract to obtain a filter residue and a third filtrate; mixing the filter residue with water at a ratio of 1:8 g / mL and subjecting the mixture to reflux extraction at a temperature of 80° C. and a time of 60 min; filtering the extract to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product;

[0061] (3) The first product and the second product are respectively placed at 6° C. for 12 h, centrifuged and filtered, and the centrifugation speed is 9000 r / min for 5 min. The products are then combined, sterilized at 70° C. for 25 min, and freeze-dried to obtain the medicinal and edible plant composition.

[0062] Example 4

[0063] The present embodiment provides a medicinal and edible plant composition, the preparation raw materials of which include: 2 parts of Pueraria root, 1 part of Hovenia dulcis, 1 part of Chrysanthemum morifolium, 1 part of Poria cocos and 1 part of Licorice.

[0064] The difference between the preparation method and Example 1 is that the raw materials are extracted in groups, which is as follows:

[0065] (1) Mixing kudzu root, poria cocos and liquorice crushed to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting to ultrasonic extraction, the ultrasonic extraction power is 200 W, the temperature is 50° C., and the time is 30 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting to reflux extraction, the temperature of the reflux extraction is 90° C., and the time is 40 min; filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0066] (2) Mixing the Hovenia dulcis and Chrysanthemum officinale crushed to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting the mixture to ultrasonic extraction. The power of the ultrasonic extraction is 200 W, the temperature is 50° C., and the time is 30 min. The extracted product is filtered to obtain a filter residue and a third filtrate. Mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting the mixture to reflux extraction at a temperature of 90° C. and a time of 40 min, the extracted product is filtered to obtain a fourth filtrate. The third filtrate and the fourth filtrate are combined to obtain a second product.

[0067] (3) The first product and the second product are respectively placed at 4° C. for 10 hours, centrifuged and filtered, and the centrifugation speed is 8000 r / min for 5 minutes. The products are then combined, sterilized at 70° C. for 25 minutes, and freeze-dried to obtain the medicinal and edible plant composition.

[0068] Example 5

[0069] The present embodiment provides a medicinal and edible plant composition, the preparation raw materials of which include: 2 parts of Pueraria root, 1 part of Hovenia dulcis, 1 part of Chrysanthemum morifolium, 1 part of Poria cocos and 1 part of Licorice.

[0070] The preparation method thereof is different from that of Example 1 only in that five raw materials are mixed and extracted simultaneously, as follows:

[0071] (1) Mixing kudzu root, Hovenia dulcis, chrysanthemum, Poria cocos and licorice crushed to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting to ultrasonic extraction, the ultrasonic extraction power is 200 W, the temperature is 50° C., and the time is 30 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting to reflux extraction, the temperature of the reflux extraction is 90° C., and the time is 40 min, filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0072] (2) The first product was allowed to stand at 4° C. for 10 hours, then centrifuged and filtered at a centrifugal speed of 8000 r / min for 5 minutes, then sterilized at 70° C. for 25 minutes, and freeze-dried to obtain the medicinal and edible plant composition.

[0073] Example 6

[0074] The present embodiment provides a medicinal and edible plant composition, the preparation raw materials of which include: 2 parts of Pueraria root, 1 part of Hovenia dulcis, 1 part of Chrysanthemum morifolium, 1 part of Poria cocos and 1 part of Licorice.

[0075] The preparation method thereof differs from that of Example 1 only in that a single reflux extraction method is adopted, which is specifically as follows:

[0076] (1) mixing kudzu root, Hovenia dulcis and chrysanthemum root crushed to 70 mesh with water at a ratio of 1:12 g / mL and performing reflux extraction, wherein the temperature of the reflux extraction is 90° C. and the time is 30 min; filtering the extract to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and performing reflux extraction, wherein the temperature of the reflux extraction is 90° C. and the time is 40 min, and filtering the extract to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0077] (2) mixing the Poria cocos and licorice powdered to 70 mesh with water at a ratio of 1:12 g / mL and subjecting the mixture to reflux extraction at a temperature of 90°C for 30 min; filtering the extract to obtain a filter residue and a third filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting the mixture to reflux extraction at a temperature of 90°C for 40 min; filtering the extract to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product;

[0078] (3) The first product and the second product are respectively placed at 4° C. for 10 hours, centrifuged and filtered, and the centrifugation speed is 8000 r / min for 5 minutes. The products are then combined, sterilized at 70° C. for 25 minutes, and freeze-dried to obtain the medicinal and edible plant composition.

[0079] Example 7

[0080] The present embodiment provides a medicinal and edible plant composition, the preparation raw materials of which include: 2 parts of Pueraria root, 1 part of Hovenia dulcis, 1 part of Chrysanthemum morifolium, 1 part of Poria cocos and 1 part of Licorice.

[0081] The preparation method thereof differs from that of Example 1 only in that a single ultrasonic extraction method is used, which is as follows:

[0082] (1) Mixing kudzu root, Hovenia dulcis and chrysanthemum root crushed to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting to ultrasonic extraction, the power of ultrasonic extraction being 200 W, the temperature being 50° C. and the time being 30 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and then subjecting to ultrasonic extraction, the power of ultrasonic extraction being 200 W, the temperature being 50° C. and the time being 40 min, filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product;

[0083] (2) Mixing the Poria cocos and licorice powdered to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting the mixture to ultrasonic extraction, wherein the power of the ultrasonic extraction is 200 W, the temperature is 50° C., and the time is 30 min; filtering the extracted product to obtain a filter residue and a third filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and then subjecting the mixture to ultrasonic extraction, wherein the power of the ultrasonic extraction is 200 W, the temperature is 50° C., and the time is 40 min; filtering the extracted product to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product;

[0084] (3) The first product and the second product are respectively placed at 4° C. for 10 hours, centrifuged and filtered, and the centrifugation speed is 8000 r / min for 5 minutes. The products are then combined, sterilized at 70° C. for 25 minutes, and freeze-dried to obtain the medicinal and edible plant composition.

[0085] Comparative Example 1

[0086] This comparative example provides a medicinal and edible plant composition, the raw materials for its preparation differ from those in Example 1 only in that chrysanthemum is missing, and the reduced amount thereof is proportionally distributed to the mass portions of kudzu root, Hovenia dulcis, Poria cocos and liquorice. The preparation method thereof is referred to Example 1.

[0087] Comparative Example 2

[0088] This comparative example provides a medicinal and edible plant composition, the raw materials of which are different from those in Example 1 in that an equal amount of chrysanthemum is replaced by wolfberry, and other components and contents remain unchanged. The preparation method thereof is referred to Example 1.

[0089] Comparative Example 3

[0090] This comparative example provides a medicinal and edible plant composition, the raw materials for its preparation differ from those in Example 1 only in the lack of Pueraria lobata, Hovenia dulcis and Chrysanthemum morifolium, and the reduced portions thereof are proportionally distributed to the mass portions of Poria cocos and Licorice.

[0091] Its preparation method is:

[0092] (1) Mixing kudzu root, Hovenia dulcis and chrysanthemum root crushed to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting to ultrasonic extraction, the ultrasonic extraction power is 200 W, the temperature is 50° C., and the time is 30 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting to reflux extraction, the temperature of the reflux extraction is 90° C., and the time is 40 min, filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a product;

[0093] (2) The product was allowed to stand at 4° C. for 10 h, then centrifuged and filtered at a centrifugal speed of 8000 r / min for 5 min, then sterilized at 70° C. for 25 min, and freeze-dried to obtain the medicinal and edible plant composition.

[0094] Comparative Example 4

[0095] This comparative example provides a medicinal and edible plant composition, the raw materials for its preparation differing from those in Example 1 only in the lack of Poria cocos and Licorice, and the reduced portions thereof are proportionally distributed to the mass portions of Pueraria lobata, Hovenia dulcis and Chrysanthemum morifolium.

[0096] Its preparation method is:

[0097] (1) Mixing Poria cocos and licorice powdered to 70 mesh with water at a ratio of 1:12 g / mL and then subjecting the mixture to ultrasonic extraction, wherein the power of the ultrasonic extraction is 200 W, the temperature is 50° C., and the time is 30 min; filtering the extracted product to obtain a filter residue and a first filtrate; mixing the filter residue with water at a ratio of 1:12 g / mL and subjecting the mixture to reflux extraction, wherein the temperature of the reflux extraction is 90° C., and the time is 40 min; filtering the extracted product to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a product;

[0098] (2) The product was allowed to stand at 4° C. for 10 h, then centrifuged and filtered at a centrifugal speed of 8000 r / min for 5 min, then sterilized at 70° C. for 25 min, and freeze-dried to obtain the medicinal and edible plant composition.

[0099] Test Example 1

[0100] Superoxide anion clearance assay:

[0101] First, the medicinal and edible plant compositions prepared in Examples 1-7 and Comparative Examples 1-4 were diluted with distilled water to a concentration of 30% to prepare sample solutions of different groups. 4.5 mL of Tris-HCl buffer (0.1 mol / L, pH=8.2), 1 mL of sample solutions of different groups, and 2.5 mL of distilled water were added to a 10 mL colorimetric tube in sequence, and allowed to stand for 10 min, 0.1 mL of pyrogallol (6 mmol / L) was added, and allowed to stand for 3 min after shaking, and 0.1 mL of HCl (10 mmol / L) was added to terminate the reaction, and the absorbance of the solution was measured at 325 nm, which was recorded as A1. The above experiment was carried out with an equal volume of distilled water instead of the sample solution as a blank group, and the absorbance was recorded as A0. According to the formula: clearance rate (%) = (A0-A1) / A0×100%, calculation was performed, and the results were measured three times in parallel. The results are shown in Table 1.

[0102] Table 1

[0103] Group Superoxide anion clearance rate (%) Example 1 69.82 Example 2 67.68 Example 3 64.91 Example 4 61.3 Example 5 60.77 Example 6 54.18 Example 7 56.60 Comparative Example 1 42.69 Comparative Example 2 40.21 Comparative Example 3 32.92 Comparative Example 4 35.83

[0104] As can be seen from the data results in Table 1, compared with the comparative example, the medicinal and edible plant composition of the present invention has a higher level of superoxide anion scavenging ability, among which Examples 1-3 have better effects, indicating that a specific preparation process can give the product better antioxidant properties. Alcohol will produce superoxide anions or other active oxygen species during human metabolism, which can damage cell membranes, proteins, etc., and have adverse effects on human health. Therefore, the medicinal and edible plant composition of the present invention also has the potential to develop products with alcohol sobering and liver protection effects.

[0105] Test Example 2

[0106] Determination of activation rate of alcohol dehydrogenase and acetaldehyde dehydrogenase:

[0107] First, the medicinal and edible plant compositions obtained in Examples 1-7 and Comparative Examples 1-4 were diluted with distilled water to a concentration of 50% to prepare sample solutions of different groups. The determination method was based on Li Fan's master's thesis "Preparation and Efficacy Study of Binhai Baishouwu Enzyme", which is as follows:

[0108] (1) Method for determining the activation rate of alcohol dehydrogenase (ADH): Add 1.5 mL of sodium pyrophosphate solution (pH 8.8) (32 mM) to a test tube, then add 1.0 mL of 0.027 mol / L oxidized coenzyme I (NAD+) (27 mM), 0.5 mL of 11.5% ethanol solution, and 0.1 mL of sample solution. Mix well and place in a 25°C water bath for 5 min. After the insulation is completed, immediately add 0.1 mL of ADH (0.25 U / mL) to the test tube. After mixing well, immediately measure the absorbance value A at a wavelength of 340 nm using a spectrophotometer. Then record A every 10 seconds for 5 minutes. Use 0.5 mL of distilled water instead of 11.5% ethanol solution as a blank control for zero adjustment, use 0.1 mL of distilled water instead of the extract as a negative control, and use the control product as a positive control. Take the initial linear part to draw a graph, with A as the ordinate and time as the abscissa, calculate the increase in A0 per minute (ΔA0) value, and calculate the activity unit of ADH based on the molar extinction coefficient of the generated NADH at 340nm of 6.2. The activity of ADH is expressed in nanomoles of NADH generated per minute (nmol / min). The ADH activity and ADH activation rate are calculated according to the following formula:

[0109] ADH activity (U / mL) = ΔA / 10s × (total reaction volume / enzyme amount) × 10 6 / (6.2×10 3 )

[0110] ADH activation rate (%) = (U1-U0) / U0×100%

[0111] Where: U1. is the ADH activity of the extract sample, and U0 is the ADH activity of the negative control group.

[0112] The results were determined three times in parallel.

[0113] (2) Method for determining the activation rate of acetaldehyde dehydrogenase (ALDH): Take a clean test tube, add 1.6 mL of sodium pyrophosphate buffer (100 mmol / L, pH 9.5), then add 1.0 mL of oxidized coenzyme I (3.6 mmol / L) and 0.1 mL of acetaldehyde (100 mmol / L), and finally add 0.1 mL of sample solution. Mix well and preheat in a 30°C water bath for 5 min. Quickly add 0.2 mL of ALDH and shake well. Immediately measure the absorbance at 340 nm and read the value every 1 min until the A is 0.1% per minute. 340 The increase in the amount of sample group ΔA remains unchanged. 340 / min, denoted as A i Replace the sample solution with 0.1 mL of distilled water and repeat the above steps to measure the ΔA of the blank group. 340 / min, recorded as A0, and the ALDH activation rate was calculated according to the following formula:

[0114] ALDH activation rate (%) = (A i -A0) / A0×100%

[0115] The results are shown in Table 2.

[0116] Table 2

[0117] Group Alcohol dehydrogenase activation rate (%) Acetaldehyde dehydrogenase activation rate (%) Example 1 90.91 83.10 Example 2 87.69 82.23 Example 3 85.33 80.86 Example 4 82.28 76.93 Example 5 80.77 72.76 Example 6 76.24 68.97 Example 7 78.64 70.22 Comparative Example 1 56.89 43.56 Comparative Example 2 53.87 39.46 Comparative Example 3 42.11 33.80 Comparative Example 4 46.87 36.41

[0118] As can be seen from the data results in Table 2, compared with the comparative example, the medicinal and edible plant composition involved in the present invention has a higher level of alcohol dehydrogenase and acetaldehyde dehydrogenase activation ability, among which the effects of Examples 1-3 are better, indicating that the specific preparation process affects the above functions of the product to a certain extent. After alcohol enters the human body, about 80% is metabolized by alcohol dehydrogenase. Acetaldehyde dehydrogenase is a key enzyme for acetaldehyde metabolism. Increased acetaldehyde dehydrogenase activity can reduce the acetaldehyde content in the blood and reduce the risk of alcohol poisoning and liver damage. Therefore, the medicinal and edible plant composition involved in the present invention also has the potential to develop products with alcohol sobering and liver protection effects.

[0119] Application Example 1

[0120] This application example provides a plant beverage, the components of which include: 6 parts of the medicinal and edible plant composition of Example 1, 3 parts of inulin, and 100 parts of drinking water.

[0121] The preparation method comprises the following steps: mixing the medicinal and edible plant composition, inulin and drinking water in proportion, homogenizing the mixture, and sterilizing the mixture to obtain the product.

[0122] Application Example 2

[0123] This application example provides a plant beverage, the components of which include: 6 parts of the medicinal and edible plant composition of Example 2, 3 parts of L-arabinose, and 100 parts of drinking water.

[0124] The preparation method comprises the following steps: mixing the medicinal and edible plant composition, L-arabinose and drinking water in proportion, homogenizing the mixture, and sterilizing the mixture to obtain the product.

[0125] Application Example 3

[0126] This application example provides a plant beverage, the components of which include: 6 parts of the medicinal and edible plant composition of Example 3, 16 parts of lemon juice, 33 parts of orange juice, and 51 parts of drinking water.

[0127] The preparation method comprises the following steps: mixing the medicinal and edible plant composition, lemon juice, orange juice and drinking water in proportion, homogenizing the mixture, and sterilizing the mixture to obtain the product.

[0128] Application Example 4-7

[0129] This application example provides four plant beverages, the components of which differ from those of Application Example 1 only in that the medicinal and edible plant composition of Example 1 is replaced by the medicinal and edible plant compositions of Examples 4-7 in equal amounts, respectively, and the other components, contents and preparation methods remain unchanged.

[0130] Comparative application example 1

[0131] This application example provides a plant beverage, the components of which differ from those of application example 1 only in that an equal amount of inulin is replaced with drinking water, and other components, contents and preparation methods remain unchanged.

[0132] Test Example 3

[0133] In this experiment, 10 technicians were selected to conduct sensory evaluation on the products of Example 1-7 and Comparative Application Example 1, including 5 males and 5 females. They scored from 4 aspects: color, taste, texture, and coordination. The evaluation results were averaged. The sensory evaluation standards and results are shown in Table 3 and Table 4:

[0134] Table 3

[0135]

[0136] Table 4

[0137]

[0138]

[0139] From the data results in Table 4, it can be seen that the taste and aroma scores of comparative application example 1 are lower because it has a distinct Chinese medicine taste, and the bitterness and astringency are more obvious, which affects the taste of the product. The products of application examples 1-7 are added with natural plant-derived excipients, which can mask the bitterness and astringency, reduce the Chinese medicine taste, give the product a special aroma, and improve the taste and aroma scores. In addition, compared with application examples 4-7, application examples 1-3 have higher scores in color and texture state, indicating that the preparation process of the composition involved in the present invention also optimizes the color and texture state of the product to a certain extent.

[0140] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0141] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0142] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A medicinal and edible plant composition, characterized in that: The medicinal and edible plant composition is a water-extracted product prepared from the following raw materials: kudzu root, Hovenia dulcis, chrysanthemum, poria and liquorice.

2. The medicinal and edible plant composition according to claim 1, characterized in that: The raw materials for preparing the medicinal and edible plant composition are calculated by weight: 1-4 parts of Pueraria root, 0.5-2 parts of Hovenia dulcis, 0.5-2 parts of Fetal Chrysanthemum, 0.5-1.5 parts of Poria and 0.5-1.5 parts of Licorice.

3. The medicinal and edible plant composition according to claim 1, characterized in that: The medicinal and edible plant composition is prepared by a preparation method comprising the following steps: (1) mixing the crushed Pueraria root, Hovenia dulcis and Chrysanthemum officinale with water and performing ultrasonic extraction, filtering the extract to obtain a filter residue and a first filtrate; mixing the filter residue with water and performing reflux extraction, filtering the extract to obtain a second filtrate; combining the first filtrate and the second filtrate to obtain a first product; (2) mixing the crushed Poria cocos and licorice with water and performing ultrasonic extraction, filtering the extract to obtain a filter residue and a third filtrate; mixing the filter residue with water and performing reflux extraction, filtering the extract to obtain a fourth filtrate; combining the third filtrate and the fourth filtrate to obtain a second product; (3) combining the first product and the second product to obtain the medicinal and edible plant composition; There is no distinction between the order of steps (1) and (2).

4. The medicinal and edible plant composition according to claim 3, characterized in that: The crushing in step (1) and step (2) means that each group is crushed independently to 40-100 mesh; Preferably, the material-liquid ratio of the plant material to water in step (1) and step (2) is independently 1:(8-18) g / mL; Preferably, the power of the ultrasonic extraction in step (1) and step (2) is independently 100-300 W, the temperature of the ultrasonic extraction is independently 40-55° C., and the time of the ultrasonic extraction is independently 20-40 min.

5. The medicinal and edible plant composition according to claim 3 or 4, characterized in that: The material-liquid ratio of the filter residue to water in step (1) and step (2) is independently 1:(8-18) g / mL; Preferably, the temperature of the reflux extraction in step (1) and step (2) is independently 80-100° C., and the time of the reflux extraction is independently 20-60 min.

6. The medicinal and edible plant composition according to any one of claims 3 to 5, characterized in that: After obtaining the first filtrate and the second filtrate in step (1), the filtrate is allowed to stand at low temperature, centrifuged and filtered, and then combined to obtain a first product; or after obtaining the first product in step (1), the filtrate is allowed to stand at low temperature, centrifuged and filtered; After obtaining the third filtrate and the fourth filtrate in step (2), the filtrate is allowed to stand at low temperature, centrifuged and filtered, and then combined to obtain the second product; or after obtaining the second product in step (1), the filtrate is allowed to stand at low temperature, centrifuged and filtered; Preferably, the low temperature standing is performed at 2-10°C for 4-12 hours; Preferably, the centrifugation is performed at a speed of 7000-10000 r / min for 3-8 min; Preferably, the medicinal and edible plant composition is also sterilized during the preparation process; Preferably, the sterilization treatment conditions are: sterilization at 60-80°C for 20-40 minutes; Preferably, in step (3), the first product and the second product are combined and then concentrated and dried.

7. Use of the medicinal and edible plant composition according to any one of claims 1 to 6 in preparing products with antioxidant effects.

8. Use of the edible and medicinal plant composition according to any one of claims 1 to 6 in the preparation of an alcohol dehydrogenase activator and / or an acetaldehyde dehydrogenase activator.

9. Use of the medicinal and edible plant composition according to any one of claims 1 to 6 in preparing a product for sobering up and protecting the liver.

10. A plant beverage, characterized in that: The components of the plant beverage include the medicinal and edible plant composition according to any one of claims 1 to 6 and auxiliary additives; Preferably, the auxiliary additive comprises any one of inulin, L-arabinose or fruit juice, or a combination of at least two thereof; Preferably, the plant beverage is prepared by mixing and homogenizing the medicinal and edible plant composition and auxiliary additives.

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