Composition and medicament for preventing and treating strawberry gray mold as well as preparation method and application of composition and medicament

Abstract

The invention belongs to the technical field of disease control, and particularly relates to a composition and a medicament for controlling strawberry gray mold as well as a preparation method and application of the composition and the medicament. The ferulic acid and the resveratrol both have a good control effect on the strawberry botrytis cinerea (botrytis cinerea), the EC50 of the ferulic acid is 40.313 mg / L, and the EC50 of the resveratrol is 26.900 mg / L. Ferulic acid and resveratrol are compounded according to the mass ratio of (1: 20)-(10: 1), a synergistic effect is shown, and the inhibition effect on strawberry botrytis cinerea (botrytis cinerea) can be further improved. The result of the embodiment shows that the ferulic acid and the resveratrol are compounded, and the co-toxicity coefficient on botrytis cinerea reaches 128.80 to 192.34. Moreover, the composition is used as an effective component for preparing a medicament, so that on the premise of synergistically preventing and treating the gray mold of the strawberries, no phytotoxicity is caused to the strawberries, and the production cost and the use cost of pesticides can be greatly reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of disease prevention and control, and specifically relates to a composition and an agent for preventing and controlling strawberry gray mold, and a preparation method and application thereof. Background Art

[0002] Strawberry is a plant of the Rosaceae family, Fragaria genus, a perennial herbaceous fruit, rich in nutrients such as protein, carbohydrates, pectin, organic acids, etc. It is a fruit with high nutritional value, high economic value, and sweet taste. It has multiple nutritional and health benefits and is deeply loved by consumers at home and abroad.

[0003] Strawberry gray mold is a fungal disease caused by Botrytis cinema, which belongs to the subphylum Ascomycota and is listed as a major disease worldwide. It is extremely harmful to strawberries grown in facilities. It is easy to occur, spreads quickly, and the pathogen is easy to develop drug resistance. Once the disease breaks out, it will cause great economic losses, reduce the yield by 40%, and in severe cases, it can cause a total loss of production.

[0004] The existing method of preventing and controlling strawberry gray mold usually uses chemical agents, such as bromoisocyanuric acid, pyrimethanil suspension, propiconazole, procymidone wettable powder and carbendazim. However, the existing antimicrobial agents have the following defects in the process of preventing and controlling strawberry gray mold: 1) After multiple frequencies and multiple doses of application, it is easy to cause strawberry gray mold fungi to develop a high degree of drug resistance (resistance), thus leading to the failure of strawberry gray mold prevention and control. 2) The amount of pesticide residues in strawberries is large, which is prone to strawberry pesticide damage, greatly hindering the improvement of strawberry quality. Summary of the invention

[0005] The purpose of the present invention is to provide a composition and an agent for preventing and controlling gray mold of strawberry, as well as a preparation method and application thereof, so as to improve the prevention and control effect of gray mold of strawberry, produce no adverse reactions to leaves, young fruits and tender branches, be safe for the growth of strawberry, and produce no phytotoxicity.

[0006] The invention provides a composition, which comprises ferulic acid and resveratrol; in the composition, the mass ratio of ferulic acid to resveratrol is (1:20) to (10:1).

[0007] Preferably, the mass ratio of ferulic acid to resveratrol in the composition is (1:15) to (7:1).

[0008] Preferably, the mass ratio of ferulic acid to resveratrol in the composition is (1:10) to (4:1).

[0009] Preferably, the mass ratio of ferulic acid to resveratrol in the composition is (1:7) to (1:1).

[0010] Preferably, the mass ratio of ferulic acid to resveratrol in the composition is 1:4.

[0011] The present invention also provides a medicament, which comprises auxiliary materials and the composition described in the above technical solution.

[0012] Preferably, the concentration of the composition in the medicament is 2 to 30 wt.%;

[0013] The auxiliary materials include one or more of a wetting agent, a dispersant, a thickener, a stabilizer, a preservative, a defoaming agent, an antifreeze agent and water;

[0014] The wetting agent includes polyether sulfonate and / or block polyether;

[0015] The dispersant includes a modified sodium salt of lignin;

[0016] The thickener includes xanthan gum and / or magnesium aluminum silicate;

[0017] The stabilizer includes white carbon black;

[0018] The preservative includes sorbic acid;

[0019] The defoamer comprises an organosilicon defoamer;

[0020] The antifreeze agent includes glycerol.

[0021] The present invention also provides a method for preparing the medicament described in the above technical solution, comprising the following steps: after mixing the auxiliary material and the composition, performing a first grinding, shearing and a second grinding process to obtain the medicament.

[0022] The present invention also provides the composition or agent described in the above technical solution or its use in inhibiting Botrytis cinerea and / or preventing and controlling strawberry gray mold.

[0023] The present invention also provides a method for preventing and controlling strawberry gray mold. In the early stage of strawberry gray mold, the agent described in the above technical solution is applied; the application amount of the agent is 1 to 15 g / mu, and the application amount of the agent is calculated based on the mass of the composition in the agent.

[0024] Beneficial effects:

[0025] Both ferulic acid and resveratrol have good control effects on strawberry gray mold (Botrytis cinerea). 50 The EC of resveratrol is 40.313 mg / L. 50It is 26.900 mg / L. The present invention compounds ferulic acid and resveratrol in a mass ratio of (1:20) to (10:1), showing a synergistic effect, which can further improve the inhibitory effect on strawberry gray mold (Botrytis cinerea). The results of the embodiment show that ferulic acid and resveratrol are compounded in a mass ratio of (1:20) to (10:1), and the co-toxicity coefficient to Botrytis cinerea reaches 128.80 to 192.34. And the preparation of the medicament with the composition of the present invention as the active ingredient will not cause phytotoxicity to strawberries under the premise of synergistically preventing and controlling strawberry gray mold, which can greatly reduce the production cost and use cost of pesticides. DETAILED DESCRIPTION

[0026] The invention provides a composition, which comprises ferulic acid and resveratrol; in the composition, the mass ratio of ferulic acid to resveratrol is (1:20) to (10:1).

[0027] In the present invention, as one embodiment, the mass ratio of ferulic acid to resveratrol in the composition is (1:15) to (7:1); as another embodiment, the mass ratio of ferulic acid to resveratrol in the composition is (1:10) to (4:1); as another embodiment, the mass ratio of ferulic acid to resveratrol in the composition is (1:7) to (1:1); as another embodiment, the mass ratio of ferulic acid to resveratrol in the composition is 1:4.

[0028] Both ferulic acid and resveratrol have good control effects on strawberry gray mold (Botrytis cinerea). When ferulic acid and resveratrol are compounded in a mass ratio of (1:20) to (10:1), a synergistic effect is exhibited, which can further enhance the inhibitory effect on strawberry gray mold (Botrytis cinerea).

[0029] The present invention also provides a medicament, which comprises auxiliary materials and the composition described in the above technical solution.

[0030] In the present invention, as one embodiment, the concentration of the composition in the medicament is 2 to 30 wt.%; as another embodiment, the concentration of the composition in the medicament is 5 to 25 wt.%; as another embodiment, the concentration of the composition in the medicament is 8 to 21 wt.%; as another embodiment, the concentration of the composition in the medicament is 11 to 16 wt.%.

[0031] In the present invention, as one embodiment, the auxiliary materials include one or more of a wetting agent, a dispersant, a thickener, a stabilizer, a preservative, a defoaming agent, an antifreeze agent and water; as another embodiment, the auxiliary materials include a wetting agent, a dispersant, a thickener, a stabilizer, a preservative, a defoaming agent, an antifreeze agent and water.

[0032] In the present invention, as an embodiment, the wetting agent includes polyether sulfonate and / or block polyether; as another embodiment, the wetting agent includes polyether sulfonate and block polyether. As an embodiment, the concentration of polyether sulfonate in the agent is 1 to 5wt.%; as another embodiment, the concentration of polyether sulfonate in the agent is 1.5wt.%. As an embodiment, the polyether sulfonate is purchased from Nanjing Lv'ai New Materials Co., Ltd. As an embodiment, the concentration of block polyether in the agent is 1 to 5wt.%; as another embodiment, the concentration of block polyether in the agent is 1.5wt.%. As an embodiment, the block polyether is purchased from Nanjing Lv'ai New Materials Co., Ltd.

[0033] In the present invention, as an embodiment, the concentration of the modified sodium lignin salt in the agent is 1-5wt.%; as another embodiment, the concentration of the modified sodium lignin salt in the agent is 2wt.%. As an embodiment, the modified sodium lignin salt is purchased from Nanjing Jierun Technology Co., Ltd.

[0034] In the present invention, as an embodiment, the thickener includes xanthan gum and / or magnesium aluminum silicate; as another embodiment, the thickener includes xanthan gum and magnesium aluminum silicate. As an embodiment, the concentration of xanthan gum in the agent is 0.1-3wt.%; as another embodiment, the concentration of xanthan gum in the agent is 0.2wt.%. As an embodiment, the xanthan gum is purchased from Guangdong Hengtian Biotechnology Co., Ltd. As an embodiment, the concentration of magnesium aluminum silicate in the agent is 0.1-3wt.%; as another embodiment, the concentration of magnesium aluminum silicate in the agent is 1wt.%. As an embodiment, the magnesium aluminum silicate is purchased from Wuhan Jiyesheng Chemical Co., Ltd.

[0035] In the present invention, as an embodiment, the stabilizer includes white carbon black. As an embodiment, the concentration of white carbon black in the agent is 0.1-2wt.%; as another embodiment, the concentration of white carbon black in the agent is 1wt.%. As an embodiment, the white carbon black is purchased from Jinan World Chemical Co., Ltd.

[0036] In the present invention, as an embodiment, the preservative includes sorbic acid. As an embodiment, the concentration of sorbic acid in the agent is 0.1-1wt.%; as another embodiment, the concentration of sorbic acid in the agent is 0.5wt.%. As an embodiment, the sorbic acid is purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0037] In the present invention, as an embodiment, the defoamer includes an organosilicon defoamer. As an embodiment, the concentration of the organosilicon defoamer in the agent is 0.1-1wt.%; as another embodiment, the concentration of the organosilicon defoamer in the agent is 0.4wt.%. As an embodiment, the organosilicon defoamer is purchased from Momentive Advanced Materials Group.

[0038] In the present invention, as an embodiment, the antifreeze agent includes glycerol. As an embodiment, the concentration of glycerol in the agent is 3-5wt.%; as another embodiment, the concentration of glycerol in the agent is 4wt.%. As an embodiment, the glycerol is purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0039] The present invention also provides a method for preparing the medicament described in the above technical solution, comprising the following steps: after mixing the auxiliary material and the composition, performing a first grinding, shearing and a second grinding process to obtain the medicament.

[0040] In the present invention, as an embodiment, the temperature of the first grinding is 0 to 30°C; as another embodiment, the temperature of the first grinding is 5 to 25°C; as another embodiment, the temperature of the first grinding is 10 to 20°C. As an embodiment, the rotation speed of the first grinding is 2000r / min. As an embodiment, the time of the first grinding is 4 to 10h; as another embodiment, the time of the first grinding is 5 to 8h. As an embodiment, the shearing speed is 1500rpm. As an embodiment, the second grinding method is: sand milling after mixing the zirconia beads and the sheared material. As an embodiment, the mass volume of the zirconia beads and the sheared material is 0.6g:1mL. As an embodiment, the sand milling uses a vertical sand mill.

[0041] The present invention uses the composition described in the technical solution as an effective ingredient and compounding it with auxiliary materials to prepare a medicament. Under the premise of synergistically preventing and controlling strawberry gray mold, it will not cause phytotoxicity to strawberries, thereby greatly reducing the production cost and use cost of pesticides.

[0042] In view of the advantages of the composition and agent provided by the present invention, the use of the composition or agent in inhibiting Botrytis cinerea and / or preventing and controlling strawberry gray mold also falls within the protection scope of the present invention.

[0043] The present invention also provides a method for preventing and controlling gray mold of strawberry. In the early stage of gray mold of strawberry, the agent described in the above technical solution is applied; the application amount of the agent is 1 to 15 g / mu, and the application amount of the agent is calculated based on the mass of the composition in the agent. As an embodiment, the application amount of the agent is 2 to 8 g / mu; as another embodiment, the application amount of the agent is 5 g / mu.

[0044] The method provided by the present invention shows obvious control effect on strawberry gray mold, the diseased fruit rate is significantly reduced, the control effect is as high as 86.58%, and there is no adverse reaction of the strawberry leaves, young fruits and tender branches to the agent. The scheme provided by the present invention is safe for strawberry growth.

[0045] To further illustrate the present invention, a composition and agent for preventing and controlling gray mold of strawberry provided by the present invention, as well as a preparation method and application thereof are described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0046] Example 1

[0047] 1. Experimental materials:

[0048] Botrytis cinereaPers, provided by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences;

[0049] 2. Experimental Reagents

[0050] Potato dextrose agar (PDA): Wash and peel the potatoes and cut them into small pieces. Weigh 200g and add 1000mL distilled water and boil for 30min. Then filter with four layers of gauze. Add 20g glucose to the filtrate. Continue heating and stirring to mix. After cooling slightly, add distilled water to 1000mL. Add 15-20g agar powder and boil. After packaging, sterilize at 121℃ for 20min and set aside after cooling.

[0051] 98wt.%Ferulic acid (original powder): purchased from Zhejiang Delekang Food Co., Ltd.

[0052] 59wt.% Resveratrol (original powder): purchased from Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.

[0053] 3. Experimental instruments and equipment

[0054] Electronic balance, beaker, culture dish, pipette, pressure steam sterilizer, clean bench, electric blast drying oven, biochemical incubator, etc.

[0055] 4. Experimental Location

[0056] Bioassay Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0057] 5. Experimental Methods

[0058] (1) Preparation of medicines

[0059] 98wt.% ferulic acid and 59wt.% resveratrol were first dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution. Single-dose mother solutions were prepared respectively, and 11 groups of proportions were designed according to the purpose of mixing and the activity of the drug. Each single dose and each group of mixed proportions were prepared in 5 series of mass concentrations according to the equal ratio method to obtain a compound drug;

[0060] (2) Target preparation

[0061] Activate Botrytis cinerea on PDA medium, and keep it aside after the mycelium fills the culture dish.

[0062] (3) Chemical treatment

[0063] In a clean bench, add 5 mL of the sterilized agent in step (1) to 45 mL of PDA culture medium cooled to 45-50°C, mix well, pour into a plate (90 mm in diameter), cool, and repeat for 3 plates (recorded as the treatment group). Use a culture medium to which an equal amount of sterile water containing the same solvent emulsifier is added instead of the sterilized agent as a control; when treating, treat the control group first, and then treat the sterilized agent in order from low concentration to high concentration. Mark each treatment immediately after completion and place them neatly.

[0064] (4) Vaccination

[0065] The bacterial mass (6 mm in diameter) from the culture dish covered with mycelium in step (2) was picked up and placed upside down in the center of the plates of the treatment group and the control group in step (3). The culture dish was sealed and cultured in a constant temperature biochemical incubator at 26°C. Each treatment was repeated 3 times, with 1 dish per repeat.

[0066] (5) Result detection

[0067] Efficacy calculation: After the colonies in the blank control have grown sufficiently, the colony diameters of each treatment were measured by the cross method, and the relative inhibition rate was calculated according to the following formula. The results were then subjected to regression analysis using SPSS20 software to obtain the EC 50 , the structure is shown in Table 1.

[0068]

[0069] Among them, colony expansion diameter (mm) = colony diameter average (mm) - 6 (mm).

[0070] Calculation of co-toxicity coefficient: The co-toxicity coefficient was calculated using the Sun Yunpei method. Ferulic acid in the mixture was used as the standard agent, and its toxicity index was 100. The co-toxicity coefficient (CTC) was calculated according to the following steps to determine the synergistic effect of the mixture:

[0071] Single-dose toxicity index = (EC 50 / Single dose EC 50 )×100;

[0072] Theoretical toxicity index (TTI) = toxicity index of single agent A × the proportion of single agent A in the mixture + toxicity index of single agent B × the proportion of single agent B in the mixture;

[0073] Measured Toxicity Index (ATI) = (EC 50 Value / EC of mixture 50 value)×100;

[0074] Co-toxicity coefficient (CTC) = (measured toxicity index / theoretical toxicity index) × 100;

[0075] Among them, when CTC is greater than 120, the mixture has synergistic effects, when CTC is less than 80, it is antagonistic, and when CTC is between 80 and 120, it is additive. The specific test results are shown in Table 1.

[0076] Table 1 Results of toxicity test of ferulic acid and resveratrol combination against Botrytis cinerea

[0077]

[0078]

[0079] According to Table 1, both single agents and their compound preparations have good control effects on strawberry gray mold (Botrytis cinerea), among which the EC of ferulic acid 50 The EC of resveratrol is 40.313 mg / L. 50 The ratio of ferulic acid to resveratrol was 26.900 mg / L. The compounding of ferulic acid and resveratrol in the ratio of (1:20) to (10:1) showed synergistic effect, among which the activity was the highest when ferulic acid:resveratrol = 1:4, and the co-toxicity coefficient was 192.34.

[0080] Example 2

[0081] Preparation of 10wt.% Ferulic Acid·1wt.% Resveratrol Suspension

[0082] (1) Preparation formula: 10wt.% ferulic acid, 1wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0083] (2) Preparation method: Weigh an appropriate amount of water, add polyether sulfonate LV-Pss, modified lignin sodium salt U3A and block polyether BC0606 according to the formula amount, add 98wt.% ferulic acid powder and 59wt.% resveratrol powder according to the formula amount after fully dissolved, then add magnesium aluminum silicate, white carbon black, glycerol, sorbic acid, silicone defoamer according to the formula amount, mix well, and then control the material temperature to 0-30°C in a sand mill, sand grind at a speed of 2000r / min, and grind for 4-10h. Slowly add xanthan gum to 10% water while stirring to prepare xanthan gum mother liquor, and then add it to the above mixture according to the formula amount for high-speed shearing at a speed of 1500rpm; then use a vertical sand mill to start grinding, sand grinding is to add zirconium oxide beads with a particle size of 0.8-1mm in the sand mill for sand grinding, the volume ratio of zirconium oxide beads to material liquid is 0.6:1, and the particle size D is detected. 90 Less than 4 microns.

[0084] Example 3

[0085] Preparation of 7wt.% Ferulic Acid·1wt.% Resveratrol Suspension

[0086] (1) Preparation formula: 7wt.% ferulic acid, 1wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0087] (2) Preparation method: same as Example 2.

[0088] Example 4

[0089] Preparation of 4wt.% Ferulic Acid·1wt.% Resveratrol Suspension

[0090] (1) Preparation formula: 4wt.% ferulic acid, 1wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0091] (2) Preparation method: same as Example 2.

[0092] Example 5

[0093] Preparation of 1wt.% Ferulic Acid·1wt.% Resveratrol Suspension

[0094] (1) Preparation formula: 1wt.% ferulic acid, 1wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0095] (2) Preparation method: same as Example 2.

[0096] Example 6

[0097] Preparation of 1wt.% Ferulic Acid·4wt.% Resveratrol Suspension

[0098] (1) Preparation formula: 1wt.% ferulic acid, 4wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0099] (2) Preparation method: same as Example 2.

[0100] Example 7

[0101] Preparation of 1wt.% Ferulic Acid·7wt.% Resveratrol Suspension

[0102] (1) Preparation formula: 1wt.% ferulic acid, 7wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0103] (2) Preparation method: same as Example 2.

[0104] Example 8

[0105] Preparation of 1wt.% Ferulic Acid·10wt.% Resveratrol Suspension

[0106] (1) Preparation formula: 1wt.% ferulic acid, 10wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0107] (2) Preparation method: same as Example 2.

[0108] Example 9

[0109] Preparation of 1wt.% Ferulic Acid·15wt.% Resveratrol Suspension

[0110] (1) Preparation formula: 1wt.% ferulic acid, 15wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0111] (2) Preparation method: same as Example 2.

[0112] Example 10

[0113] Preparation of 1wt.% Ferulic Acid·20wt.% Resveratrol Suspension

[0114] (1) Preparation formula: 1wt.% ferulic acid, 20wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0115] (2) Preparation method: same as Example 2.

[0116] Comparative Example 1

[0117] Preparation of 5wt.% Ferulic Acid Suspension

[0118] (1) Preparation formula: 5wt.% ferulic acid, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0119] (2) Preparation method: same as Example 2.

[0120] Comparative Example 2

[0121] Preparation of 5wt.% Resveratrol Suspension

[0122] (1) Preparation formula: 5wt.% resveratrol, 1.5wt.% polyether sulfonate LV-Pss, 2wt.% modified lignin sodium salt U3A, 1.5wt.% block polyether BC0606, 0.2wt.% xanthan gum, 1wt.% magnesium aluminum silicate, 1wt.% white carbon black, 4wt.% glycerol, 0.5wt.% sorbic acid, 0.4wt.% silicone defoamer and the balance water.

[0123] (2) Preparation method: same as Example 2.

[0124] Test Example 1

[0125] With reference to NY / T 2989-2016 Pesticide Registration Product Specification System, the technical indexes of the suspension concentrates of Examples 2 to 10 and Comparative Examples 1 and 2 were tested, and all met the requirements of Table 2.

[0126] Table 2 Control indicators of different suspension agents

[0127]

[0128] Test Example 2

[0129] Field test on the efficacy of pesticides against gray mold of strawberry

[0130] 1. Test crops: Strawberry (variety: Hongyan).

[0131] 2. Test object: Strawberry gray mold (pathogen: Botrytis cinereaPers).

[0132] 3. Test location: Strawberry Garden, Fuxing Road, Mancheng District, Baoding City, Hebei Province. The soil fertility of the test site is medium, the terrain is flat, the fertility is uniform, and the irrigation conditions are good.

[0133] 4. Test treatment: According to the guidelines for field efficacy tests of pesticides (II) Part 120 Fungicides for the control of strawberry gray mold GB / T 17980.120-2004.

[0134] The experimental site was randomly divided into a blank control area and a treatment area. Each plot was arranged in a randomized block group with an area of ​​20m 2 , repeated 4 times, applied at the early stage of strawberry gray mold, and applied once during the entire test. The treatment area was applied with the suspension concentrates of Examples 2 to 10 and Comparative Examples 1 and 2, and the application amount was 5 g / mu based on the mass of the active ingredients (ferulic acid and resveratrol); the blank control area was applied with clean water.

[0135] 5. Investigation method: Investigate the disease base number before applying the pesticide, sample five points on the diagonal of each plot, investigate 50 fruits at each point, and record the total number of fruits and the number of diseased fruits. Investigate the control effect 7 days after applying the pesticide.

[0136] 6. Drug efficacy evaluation: The drug efficacy was calculated according to the following formula. The results are shown in Table 3.

[0137]

[0138] Table 3 Results of field efficacy test on strawberry gray mold

[0139] Drug Name Diseased fruit rate (%) Control effect (%) Example 2 19.60 61.54d Example 3 18.44 65.45d Example 4 12.10 74.34bc Example 5 11.50 72.42bc Example 6 7.70 86.58a Example 7 12.20 78.13b Example 8 13.30 76.23b Example 9 14.20 73.45bc Example 10 15.90 72.53bc Comparative Example 1 23.40 52.38e Comparative Example 2 19.40 67.55cd Blank control 53.90 -

[0140] Note: The diseased fruit rate and control efficacy in the table are the average values ​​of 4 repeated groups, and lowercase letters represent significant differences at the 5% level.

[0141] According to Table 3, the mixed agents of Examples 2 to 10 all showed obvious control effects on strawberry gray mold. Compared with the blank control, the diseased fruit rate was significantly reduced. Compared with the single-dose comparative example 1 and the single-dose comparative example 2, the control effects were significantly different. Among them, Example 6 had the best control effect, reaching 86.58%, with obvious synergistic effect. In addition, no adverse reactions to the agents occurred in leaves, young fruits, and tender branches during the test, and the strawberry growth was safe.

[0142] Based on the above content, it can be seen that the combination of ferulic acid and resveratrol can reduce production costs and usage costs when synergistically preventing and controlling strawberry gray mold, will not cause phytotoxicity to strawberries, and is safe for strawberries.

[0143] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A composition, characterized in that The composition comprises ferulic acid and resveratrol; the mass ratio of ferulic acid to resveratrol in the composition is (1:20) to (10:1).

2. The composition according to claim 1, characterized in that The mass ratio of ferulic acid to resveratrol in the composition is (1:15) to (7:1).

3. The composition according to claim 2, characterized in that The mass ratio of ferulic acid to resveratrol in the composition is (1:10) to (4:1).

4. The composition according to claim 3, characterized in that The mass ratio of ferulic acid to resveratrol in the composition is (1:7) to (1:1).

5. The composition according to claim 4, characterized in that The mass ratio of ferulic acid to resveratrol in the composition is 1:

4.

6. A medicament, characterized in that: The medicament comprises excipients and the composition according to any one of claims 1 to 5.

7. The medicament according to claim 6, characterized in that The concentration of the composition in the medicament is 2 to 30 wt.%; The auxiliary materials include one or more of a wetting agent, a dispersant, a thickener, a stabilizer, a preservative, a defoaming agent, an antifreeze agent and water; The wetting agent includes polyether sulfonate and / or block polyether; The dispersant includes a modified sodium salt of lignin; The thickener includes xanthan gum and / or magnesium aluminum silicate; The stabilizer includes white carbon black; The preservative includes sorbic acid; The defoamer comprises an organosilicon defoamer; The antifreeze agent includes glycerol.

8. The method for preparing the medicament according to claim 6 or 7, characterized in that: The method comprises the following steps: after mixing the auxiliary material and the composition, performing a first grinding, shearing and a second grinding process to obtain the medicament.

9. Use of the composition according to any one of claims 1 to 5, the agent according to claim 6 or 7, or the agent obtained by the preparation method according to claim 8 in inhibiting Botrytis cinerea and / or preventing and controlling gray mold of strawberry.

10. A method for preventing and controlling gray mold of strawberry, characterized in that: At the early stage of strawberry gray mold disease, apply the agent described in claim 6 or 7 or the agent obtained by the preparation method described in claim 8; the application amount of the agent is 1 to 15 g / mu, and the application amount of the agent is calculated based on the mass of the composition in the agent.

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