Golden tea composition with whitening and repairing effects and application

Abstract

The invention relates to the field of cosmetics, in particular to a platinum tea composition with whitening and repairing effects and application. The platinum tea composition is prepared from the following components in parts by weight: 5 to 15 parts of golden flower white tea fermentation product filtrate, 1 to 6 parts of carob seed extract and 1 to 2 parts of decarboxylated carnosine hydrochloride. The golden flower white tea fermentation product filtrate, the carob seed extract and the decarboxylated carnosine hydrochloride are compounded, the synergistic effect is achieved, the whitening, antioxidant and repairing effects of the composition can be effectively enhanced, and the cosmetics prepared by adding the composition into the cosmetics are safe, high in stability and good in transdermal absorbability and have the efficient whitening and repairing effects.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a platinum tea composition with whitening and repairing effects and an application thereof. Background Art

[0002] At present, scientific research on white tea mainly focuses on the fields of physical and chemical component analysis, antibacterial, antimutagenic, anticancer, and obesity prevention, while there are few studies on white tea's in vitro antioxidant and skin anti-aging effects. There is a lack of application in cosmetics, and its skin care value has not yet been fully utilized. Although tea polyphenols have good skin care effects, they cannot fully exert their maximum skin care effects due to their poor stability and relatively limited application concentration. Relying solely on the antioxidant components of white tea itself may have problems such as single effect and inability to meet complex skin needs. Although carob seed extract has the effect of activating the skin's antioxidant enzyme system, it faces the problem that the active ingredients are easily affected by factors such as light, temperature, and pH value and are easily deactivated. It is necessary to develop appropriate formulas and technologies to ensure its stability, so as to give full play to its antioxidant effect and meet the use needs of skin care products under different environmental conditions. The role of decarboxylated carnosine hydrochloride in single anti-glycation may not be able to comprehensively improve the overall condition of the skin. It needs to be used in conjunction with other ingredients with different effects to achieve better skin care effects. How to further optimize its application in skin care products, improve its anti-glycation effect and ensure safety is a technical problem that needs to be solved urgently. Summary of the invention

[0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a platinum tea composition with high-efficiency whitening and high-efficiency repairing effects.

[0004] To achieve the above object, the technical solution adopted by the present invention is:

[0005] In a first aspect, the present invention provides a platinum tea composition, comprising the following components in parts by weight: 5-15 parts of filtrate of chrysanthemi white tea fermentation product, 1-6 parts of carob seed extract, and 1-2 parts of decarboxylated carnosine hydrochloride.

[0006] Old white tea, also known as platinum tea, is white tea that has been stored for many years. The "many years" here means within a reasonable shelf life, such as 10 to 20 years. White tea is a slightly fermented tea. Studies have shown that the flavonoids in white tea are higher than those in other teas, making it a special treasure among Chinese teas. Fresh white tea leaves undergo solid-state aerobic fermentation to obtain the fermentation filtrate of golden flower white tea. The polyphenolic compounds and flavonoid compounds contained in white tea have strong antioxidant activity and can remove free radicals in the body. Tea polyphenols, also known as antioxidant spirits, are a class of polyhydroxy compounds contained in tea leaves. They have strong antioxidant and physiological activity and are free radical scavengers in the human body. One milligram of tea polyphenols can remove excess free radicals that are harmful to the human body, which is equivalent to 9 micrograms of superoxide dismutase (SOD), which is much higher than other similar substances. Carob seed extract can activate the antioxidant enzyme system in the skin, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). These enzymes can catalyze the decomposition of free radicals, reduce the concentration of free radicals, and protect skin cells from oxidative damage. Decarboxylated carnosine hydrochloride can inhibit glycosylation reaction. During the glycosylation reaction, some active carbonyl compounds, such as methylglyoxal (MGO) and glyoxal, etc., are produced. These compounds react with biological macromolecules such as proteins and nucleic acids, resulting in the formation of glycosylation end products (AGEs). Decarboxylated carnosine hydrochloride has a strong nucleophilicity and can be combined with active carbonyl compounds to form a stable complex, thereby preventing them from reacting with biological macromolecules and reducing the generation of AGEs. The present invention compounds the filtrate of the fermentation product of golden flower white tea, the extract of long beans seeds, and the decarboxylated carnosine hydrochloride, which has a synergistic effect, can solve the shortcomings of the three components being applied to cosmetics separately, and enables the three components to exert the maximum skin care effect. The three components of golden flower white tea fermentation product filtrate, long bean seed extract and decarboxylated carnosine hydrochloride all have strong antioxidant properties. Decarboxylated carnosine hydrochloride can inhibit glycation reactions. The combination of the three has the efficacy of high-efficiency whitening and high-efficiency repair, and the antioxidant effect is more comprehensive, bringing new possibilities for the application of cosmetics.

[0007] Preferably, the platinum tea composition comprises the following components in parts by weight: 7-15 parts of filtrate of chrysanthemi white tea fermentation product, 3-6 parts of carob seed extract, and 1.4-2 parts of decarboxylated carnosine hydrochloride.

[0008] More preferably, the platinum tea composition comprises the following components in parts by weight: 15 parts of filtrate of chrysanthemi white tea fermentation product, 5 parts of carob seed extract, and 2 parts of decarboxylated carnosine hydrochloride.

[0009] In a second aspect, the present invention provides use of the platinum tea composition in cosmetics.

[0010] In a third aspect, the present invention provides a cosmetic comprising the above-mentioned platinum tea composition.

[0011] Preferably, the platinum tea composition is added in an amount of 7.00%-23.00% in cosmetics.

[0012] More preferably, the platinum tea composition is added in an amount of 22.00% in cosmetics.

[0013] Preferably, the dosage form of the cosmetic includes water, lotion, and cream.

[0014] Preferably, the cosmetics also include auxiliary materials acceptable in daily chemical products.

[0015] Preferably, the auxiliary material includes at least one of an emollient, a dispersant, a humectant, a preservative, a thickener, and a pH adjuster.

[0016] The beneficial effects of the present invention are:

[0017] The invention compounds filtrate of golden flower white tea fermentation product, carob seed extract and decarboxylated carnosine hydrochloride, has synergistic effect, can solve the disadvantages of the three components being applied to cosmetics separately, and enables the three components to exert maximum skin care effect.

[0018] The three components of golden flower white tea fermentation product filtrate, long bean seed extract and decarboxylated carnosine hydrochloride all have strong antioxidant properties. Decarboxylated carnosine hydrochloride can inhibit glycation reactions. The combination of the three has the efficacy of high-efficiency whitening and high-efficiency repair, and the antioxidant effect is more comprehensive, bringing new possibilities for the application of cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is the platinum tea composition sample of Example 10. DETAILED DESCRIPTION

[0020] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0021] The raw materials used in the present invention come from:

[0022] The filtrate of Jinhua white tea fermentation product was purchased from Bozhou Mingqi Biotechnology Co., Ltd.; the longan seed extract was purchased from Guangzhou Fuzhilin Biotechnology Co., Ltd.; and the decarboxylated carnosine hydrochloride was purchased from Zhejiang Paipeptide Biotechnology Co., Ltd.

[0023] The appearance of the platinum tea composition sample prepared by the present invention is as follows Figure 1 As shown (taking Example 10 as an example).

[0024] After the cosmetics prepared by the present invention are prepared, the materials are discharged and left to stand, and semi-finished products are inspected; after the inspection is qualified, the cosmetics are filled and packaged; and finally, the finished product is inspected to obtain the final product.

[0025] Embodiment 1-12:

[0026] An embodiment of the platinum tea composition of the present invention; the components by weight of the platinum tea composition are shown in Table 1.

[0027] Table 1 Components by weight of the platinum tea composition described in each embodiment

[0028]

[0029]

[0030] Comparative Examples 1-4:

[0031] Comparative Examples 1-4 are comparative examples of the platinum tea composition of the present invention; wherein Comparative Example 4 is a green tea composition.

[0032] The weight parts of the components of each comparative example composition are shown in Table 2.

[0033] Table 2 Components by weight of each comparative example composition

[0034]

[0035] Among them, the only difference between Comparative Examples 1-4 and Example 10 is that: Comparative Example 1 does not add the filtrate of the fermentation product of golden flower white tea; Comparative Example 2 does not add the longan seed extract; Comparative Example 3 does not add decarboxylated carnosine hydrochloride; the missing components are replaced by an equal weight of water; Comparative Example 4 replaces the filtrate of the fermentation product of golden flower white tea with an equal weight of the filtrate of the fermentation product of green tea.

[0036] Application Examples 1-12:

[0037] The application example of the cosmetics of the present invention; the cosmetics is specifically a facial cream; the cosmetics includes a platinum tea composition.

[0038] The formula of the cosmetic is shown in Table 3; the content of the platinum tea composition added in each application example is shown in Table 4.

[0039] Table 3 Formula of the cosmetics described in Application Example 1

[0040]

[0041]

[0042] Table 4 Content of platinum tea composition added in each application example

[0043]

[0044]

[0045] The preparation method of the cosmetic comprises the following steps:

[0046] (1) Add appropriate amount of water, glycerin and carbomer into an emulsifier in sequence, stir at 800-1200 rpm, then homogenize at 2000-2500 rpm for 1-3 min, heat to 80-85° C., and keep warm for 10-15 min until completely dissolved without gel;

[0047] (2) Add caprylic / capric triglyceride, cetearyl alcohol, C10-18 fatty acid triglycerides, glyceryl stearate / PEG-100 stearate, shea butter, and polydimethylsiloxane into the oil phase pot, stir at 800-1200 rpm, raise the temperature to 80-85° C., homogenize at 800-1200 rpm for 5-8 minutes, and stir to completely dissolve;

[0048] (3) Cool down to 50-55°C, add pre-dispersed butanediol and p-hydroxyacetophenone in sequence, stir at 800-1200 rpm, add polyacrylamide / C13-14 isoparaffin / laureth-7, homogenize at 800-1200 rpm for 5-8 minutes, and stir until completely dissolved.

[0049] (4) Cool down to 45°C, add the dispersed aminomethyl propanol that has been heated and dissolved at 50-60°C, stir evenly at 800-1200 rpm for 3-8 minutes, then add the remaining water and stir evenly at 800-1200 rpm until it is completely emulsified and there is no oil on the surface, then start cooling.

[0050] (5) Add the pre-dispersed platinum tea composition and phenoxyethanol in small amounts and multiple times, stir evenly at 600-1000 rpm, cool to 38° C., filter the material (100 mesh filter bag) after the central control is qualified, and obtain the cosmetics.

[0051] Application Comparative Example 1:

[0052] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically a facial cream; the difference between this application comparison example and application example 10 is that the platinum tea composition of example 10 contained in application example 10 is replaced with an equal amount of the platinum tea composition of comparison example 1, and the other components are the same as application example 10.

[0053] The preparation method of the cosmetic is the same as that of the application example.

[0054] Application Comparative Example 2:

[0055] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically a facial cream; the difference between this application comparison example and application example 10 is that the platinum tea composition of example 10 contained in application example 10 is replaced with an equal amount of the platinum tea composition of comparison example 2, and the other components are the same as application example 10.

[0056] The preparation method of the cosmetic is the same as that of the application example.

[0057] Application Comparative Example 3:

[0058] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically a facial cream; the difference between this application comparison example and application example 10 is that the platinum tea composition of example 10 contained in application example 10 is replaced with an equal amount of the platinum tea composition of comparison example 3, and the other components are the same as application example 10.

[0059] The preparation method of the cosmetic is the same as that of the application example.

[0060] Application Comparative Example 4:

[0061] An application comparison example of the cosmetic of the present invention; the cosmetic is specifically a facial cream; the difference between this application comparison example and application example 10 is that 22.00% of the platinum tea composition in application example 10 is replaced with 22.00% of the green tea composition of comparison example 4, and the remaining components are the same as application example 10.

[0062] The preparation method of the cosmetic is the same as that of the application example.

[0063] Application Comparative Example 5:

[0064] An application comparison example of the cosmetics of the present invention; the cosmetics are specifically a facial cream; the difference between this application comparison example and application example 10 is only that: the platinum tea composition in application example 10 is replaced with an equal amount of water, and the other components are the same as application example 10.

[0065] The preparation method of the cosmetic is the same as that of the application example.

[0066] Test Example 1: Safety Patch Test

[0067] Test sample: a facial cream sample containing the platinum tea composition prepared in Example 10.

[0068] Subjects: 9 males and 26 females, a total of 35 people, aged 22 to 45 years old, with an average age of 26.1±5.6 years old, who met the voluntary inclusion criteria.

[0069] The spot test method is as follows:

[0070] Select qualified patch test equipment and use the closed patch test method to place the patch product containing the test sample (cut into the size of the patch tester, about 50mm 2 ) was placed in a patch tester, and a hypoallergenic tape was applied to the back of the subject. After 24 hours, the test sample was removed, and the skin reaction was observed at 0.5, 24, and 48 hours after removal. A negative control (blank, no treatment) was set up at the same time. The results were recorded according to the skin reaction grading standard of the skin occlusion patch test in Chapter 7 of the "Technical Specifications for Safety of Cosmetics" (2015 edition). The safety patch test results are shown in Table 5.

[0071] Table 5

[0072]

[0073] The results of human skin patch tests showed that among 35 subjects, no adverse skin reactions occurred, indicating that the platinum tea composition prepared by the present invention is highly safe.

[0074] Test Example 2: Antioxidant Test (DPPH Method)

[0075] Test samples: platinum tea compositions prepared in Examples 1-12 and Comparative Examples 1-4, and blank control.

[0076] Test principle: The test is based on the characteristics of DPPH free radicals, which have a single electron, strong absorption at 517nm, and purple alcohol solution. When there is a free radical scavenger, it pairs with the single electron in the DPPH free radical, causing its absorption to gradually disappear. The degree of fading is quantitatively related to the number of electrons it accepts, so a spectrophotometer can be used for rapid quantitative analysis.

[0077] 1. Reagents and materials:

[0078] (1.1) Reagents:

[0079] 0.2 mM DPPH solution (1,1-diphenyl-2-trinitrophenylhydrazine, C 18 H 12 N5O6): Weigh 0.007875 g of DPPH powder and dissolve it in 100 mL of anhydrous ethanol to prepare a 0.2 mM DPPH solution.

[0080] Vitamin C standard control solution (positive control group): dilute VC with deionized water to 0.001 mg / mL, 0.002 mg / mL, 0.004 mg / mL, 0.006 mg / mL, 0.008 mg / mL, 0.01 mg / mL, 0.02 mg / mL and 0.5 mg / mL in sequence and place in a brown bottle for later use.

[0081] (1.2) Instruments and equipment: electronic balance (brand: Sartiorius, accurate to 0.001 g), UV spectrophotometer (brand: HITACHI).

[0082] 2. Test steps:

[0083] (2.1) Test four sample tubes and add samples in sequence according to Table 6.

[0084] Table 6

[0085] Reagents T-sample tube <![CDATA[T0 - Sample Background]]> C—DPPH tube <![CDATA[C0 - Solvent background]]> Sample solution (mL) 2 2 / / Water or 95% ethanol solvent (mL) / 2 2 2 DPPH ethanol solution (mL) 2 / 2 /

[0086] (2.2) After mixing the solutions in each tube, react them at room temperature in the dark for 30 minutes, adjust the wavelength to zero with distilled water at 517nm, measure the absorbance with a spectrophotometer, and record the data (in data processing, it should be noted that the absolute difference between two independent measurement results obtained under repeatability conditions should not exceed 10% of the arithmetic mean). Calculate the clearance rate (P) according to formula (1).

[0087]

[0088] Where:

[0089] T—absorbance value of sample tube, i.e. absorbance value of solution after reaction between sample and DPPH;

[0090] T0—sample background absorbance;

[0091] C—DPPH tube absorbance, that is, the absorbance of DPPH solution without adding sample;

[0092] C0—Solvent background absorbance.

[0093] 3. Experimental results:

[0094] Table 7

[0095]

[0096]

[0097] The experimental results are shown in Table 7. The antioxidant experiment verified that the platinum tea compositions prepared in the embodiments of the present invention all have good free radical scavenging and antioxidant effects. Among them, the platinum tea composition of Example 10 has the best antioxidant effect, indicating that the increase in the content of the filtrate of the fermentation product of golden flower white tea helps it to synergize with other components and improve the antioxidant effect of the platinum tea composition.

[0098] Test Example 3: Test for inhibition of tyrosinase activity

[0099] Test samples: platinum tea compositions prepared in Examples 1-12 and Comparative Examples 1-4, and blank control.

[0100] Experimental principle: Tyrosinase is the rate-limiting enzyme in the melanin synthesis pathway, which mainly affects the production of melanin by affecting the conversion of tyrosine to DOPA and the oxidation of DOPA to DOPAQUINONE. The principle is that tyrosine or DOPA is converted into DOPAQUINONE under the action of tyrosinase. This reaction is a color reaction, which is determined by colorimetry to determine the inhibition rate of different brightening agents on tyrosinase activity.

[0101] 1. Reagents and materials:

[0102] (1.1) Reagents: tyrosinase (25 kU), L-dopa, ≥98%, α-arbutin, ≥99%

[0103] (1.2) Reagent preparation:

[0104] (1.2.1) Preparation of 0.05 mol / L PBS (pH = 6.8):

[0105] Solution A: Weigh 7.099 g of disodium hydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L Na2HPO4 solution;

[0106] Solution B: Weigh 6.803 g of potassium dihydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L KH2PO4 solution;

[0107] Take 50mL of liquid A and 50mL of liquid B and mix them.

[0108] (1.2.2) Tyrosinase solution: Prepare tyrosinase solution to 100 U / mL in PBS buffer at pH 6.8.

[0109] (1.2.3) DOPA solution: Weigh 0.04 g DOPA and dissolve it in 40 mL PBS buffer (pH 6.8) and store in a dark place.

[0110] (1.2.4) α-Arbutin: Dilute α-Arbutin to 100 μM using PBS buffer at pH 6.8.

[0111] 2. Instruments and equipment: analytical balance (accurate to 0.0001 g), 1L volumetric flask, 50mL measuring cylinder, 1000μL pipette, constant temperature water bath, UV-visible spectrophotometer.

[0112] 3. Determination steps

[0113] Prepare the test solution according to Table 8:

[0114] Add the test sample, 0.05M PBS buffer (pH 6.8), and tyrosinase solution into the test tube in sequence;

[0115] Table 8 Reaction solution composition (mL)

[0116]

[0117] After preparing the test solution, place the test tube in a 37°C water bath for 10 minutes; then, add 2 mL of DOPA solution, react for 5 minutes, and measure the absorbance at 475 nm. The absolute difference between two independent determination results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean. Calculate the tyrosinase inhibition rate (%) according to formula (2).

[0118] Tyrosinase inhibition rate (%) = [1-(OD C -OD D ) / (OD A -OD B )]×100%——Formula (2)

[0119] In the above formula, OD A is the absorbance value of the enzyme solution control group; OD B is the absorbance value of the enzyme solution control blank group; OD C is the absorbance value of the sample group; OD D is the absorbance value of the blank control of the sample group.

[0120] Table 9

[0121] Test samples Tyrosinase inhibition rate (%) Example 1 18.76 Example 2 20.45 Example 3 21.21 Example 4 23.00 Example 5 21.76 Example 6 22.36 Example 7 23.65 Example 8 20.82 Example 9 23.22 Example 10 24.32 Embodiment 11 23.44 Example 12 19.54 Comparative Example 1 2.87 Comparative Example 2 9.76 Comparative Example 3 10.43 Comparative Example 4 16.46 Blank control (water) -5.09

[0122] The test results are shown in Table 9. Compared with the comparative example, the platinum tea composition prepared in the embodiment of the present invention has significant tyrosinase inhibition activity, that is, has good whitening and brightening effect. Among them, the platinum tea composition of Example 10 has the best effect, that is, the optimal combination ratio is filtrate of golden flower white tea fermentation product: long bean seed extract: decarboxylated carnosine hydrochloride = 15:0.1:1.

[0123] Test Example 4: Repair performance test (cell migration experiment)

[0124] Experimental principle: The ability of cell migration directly reflects the activity level of cells. If cells can proliferate and migrate quickly, they can form a protective mechanism as soon as possible in response to exogenous stimuli, thereby repairing damaged cells. The cell scratch (repair) method is a simple method to measure cell migration and repair ability. It is similar to the in vitro wound healing model. On a monolayer of adherent cells cultured in an in vitro culture dish or plate, a line is drawn in the central area of ​​cell growth with a micropipette tip or other hard objects to remove the cells in the central part. Then, the cells are cultured for the time set in the experiment, and the cell culture plate is removed to observe whether the surrounding cells grow (repair) to the central scratch area. In this way, the repair ability of the cells is judged. The experiment usually requires the setting of a normal control group and an experimental group. The experimental group is a group to which certain treatment factors, drugs, exogenous genes, etc. are added. The ability of cells in different groups to repair the scratch area can be used to judge the migration and repair ability of each group of cells.

[0125] 1. Experimental materials:

[0126] (1) Equipment: cell counter;

[0127] (2) Reagents: culture medium, trypsin, trypan blue, PBS;

[0128] (3) Consumables: cell counting chamber, transwell plate.

[0129] 2. Prepare reagents for test group treatment:

[0130] (1) Negative control: DMEM culture medium containing 10% serum.

[0131] (2) Sample group: the platinum tea compositions of Examples 1-12 and Comparative Examples 1-4, and a blank control; the platinum tea composition was prepared into a 2% by mass solution using DMEM culture medium containing 10% serum as a solvent.

[0132] (3) Positive control: Use DMEM culture medium containing 10% serum as solvent to prepare a 10% by mass epidermal growth factor (EGF) solution.

[0133] 3. Test steps:

[0134] (1) Cell inoculation and culture: Inoculate the cell suspension with adjusted concentration into a pre-prepared culture plate (12-well plate), add an appropriate volume (e.g., 2 mL or 1 mL) of cell suspension to each well, ensure that the cells are evenly distributed at the bottom of the well, and culture overnight in a 37°C, 5% CO2 incubator to allow the cells to adhere to the wall and grow to form a monolayer of cells.

[0135] (2) Scratch operation: Take out the culture plate and use a sterile micropipette tip or a special scratching tool in an ultra-clean workbench to gently scratch a straight line in the same direction in the central area of ​​the monolayer of adherent cells. The width of the scratch should be kept relatively consistent (about 0.5-1 mm), and try to avoid scratching the bottom of the culture plate.

[0136] Wash the cells gently 2-3 times with PBS to remove the scratched cell debris, then replace with serum-free DMEM culture medium and continue to culture in the incubator for 0-2 hours (starvation treatment, the time can be adjusted according to the cell type) to synchronize the cells in the stationary phase and reduce the effect of serum on cell migration.

[0137] (3) After the starvation treatment, carefully remove the serum-free DMEM culture medium and add samples according to the experimental design:

[0138] Negative control group: DMEM culture medium containing 10% serum was added.

[0139] Sample group: a solution prepared by adding the platinum tea composition of Examples 1-12 and Comparative Examples 1-4 at a mass percentage of 2% (using DMEM culture medium containing 10% serum as solvent).

[0140] Positive control group: 10% by mass of epidermal growth factor (EGF) solution (using DMEM culture medium containing 10% serum as solvent) was added.

[0141] The sample should be added slowly dropwise to avoid dispersing cells or causing excessive local concentration.

[0142] (4) The culture plates were taken out at set time points of 0 hour (i.e., immediately after adding the sample) and 24 hours after culture for observation and photographing, and three areas were randomly selected to calculate the mean scratch area between cells. The cell migration rate (wound healing rate) was calculated by the mean scratch area between cells at 0 hour and 18 hour, and the repair effect of the sample was expressed by the cell migration rate (wound healing rate). The larger the cell migration rate (wound healing rate), the better the repair effect.

[0143] The cell migration rate (wound healing rate) % was calculated according to formula (3).

[0144] Cell migration rate (%) = (initial scratch width or area - scratch width or area at observation time point) / initial scratch width or area × 100%

[0145] ——Formula (3)

[0146] Table 10

[0147]

[0148]

[0149] The test results are shown in Table 10. Compared with the blank control group, the platinum tea compositions prepared in the embodiments of the present invention and the comparative examples all have certain repairing abilities and can promote the migration of HaCaT human keratinocytes. Among them, the repairing effect of the platinum tea compositions of Examples 1-12 is better than that of the platinum tea compositions of Comparative Examples 1-4, indicating that the compounding of the filtrate of the fermentation product of golden flower white tea, the extract of long bean seeds, and decarboxylated carnosine hydrochloride has a synergistic effect and can effectively enhance the repairing effect of the final product. Without any of the components, the repairing effect of the obtained platinum tea compositions is poor. Among them, the platinum tea composition of Example 10 has the best effect, that is, the optimal combination ratio is the filtrate of the fermentation product of golden flower white tea: the extract of long bean seeds: the decarboxylated carnosine hydrochloride = 15:5:2.

[0150] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A platinum tea composition, characterized in that: The invention comprises the following components in parts by weight: 5-15 parts of filtrate of chrysanthemi chinensis fermentation product, 1-6 parts of carob seed extract and 1-2 parts of decarboxylated carnosine hydrochloride.

2. The platinum tea composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 7-15 parts of filtrate of chrysanthemi chinensis fermentation product, 3-6 parts of carob seed extract and 1.4-2 parts of decarboxylated carnosine hydrochloride.

3. The platinum tea composition according to claim 2, characterized in that The invention comprises the following components in parts by weight: 15 parts of filtrate of chrysanthemi chrysanthemi fermentation product, 5 parts of carob seed extract and 2 parts of decarboxylated carnosine hydrochloride.

4. Use of the platinum tea composition according to any one of claims 1 to 3 in cosmetics.

5. A cosmetic, characterized in that: The invention comprises the platinum tea composition as described in any one of claims 1 to 3.

6. The cosmetic according to claim 5, characterized in that The addition amount of the platinum tea composition in cosmetics is 7.00%-23.00%.

7. The cosmetic according to claim 6, characterized in that The addition amount of the platinum tea composition in cosmetics is 22.00%.

8. The cosmetic according to claim 5, characterized in that The dosage forms of the cosmetics include water, milk and cream.

9. The cosmetic according to claim 5, characterized in that: The cosmetics also include auxiliary materials acceptable in daily chemical products.

10. The cosmetic according to claim 9, characterized in that The auxiliary materials include at least one of an emollient, a dispersant, a humectant, a preservative, an emulsion stabilizer, a thickener, and a pH regulator.

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