Intelligent device for applying periodic pressure to cells

Abstract

The invention relates to the technical field of cell testing, in particular to an intelligent device for applying periodic pressure to cells, which comprises a pressure applying mechanism, the pressure applying mechanism comprises a base, a dust cover is arranged on the base, a detection piece is arranged on the base, a transfer mechanism is arranged on the dust cover, and the transfer mechanism is arranged on the detection piece. The transfer mechanism comprises a transfer hole formed in the dust cover, a transfer pipe is fixedly connected into the transfer hole and extends upwards out of the dust cover, a sealing cover is in threaded connection with the top end of the transfer pipe, a transfer box is arranged on the inner wall of the dust cover, and a communicating pipe is arranged at the top end of the transfer box in a communicating mode. And the communicating pipe can be communicated with the transfer hole.

Description

Technical Field

[0001] The invention relates to the technical field of cell testing, and in particular to an intelligent device for applying periodic pressure to cells. Background Art

[0002] Cells are very sensitive to mechanical stress in the surrounding environment, and they respond to external mechanical stimuli mainly through ion channels on the cell membrane, extracellular matrix and cytoskeleton. The response of cells to mechanical stimuli can be divided into proliferation, differentiation, morphological changes and apoptosis. Exploring the effects of mechanical stimuli under different conditions on cell behavior is a very important basic scientific issue.

[0003] At present, the mechanical forms that can stimulate cells can be divided into cyclic tensile stress, fluid shear stress, continuous air compression force and periodic liquid perfusion pressure. Since cell experiments require repeated testing, a simple and portable device with easy operation during the stress application process is required.

[0004] Although existing devices are also capable of applying periodic pressure to cells, these devices cannot be used in conjunction with cell storage devices. Cells usually need to be removed from the cell storage device and then moved to a pressure-applying device. During the transportation process, cells are easily exposed to the external environment for a long time, affecting the state of the cells.

[0005] Therefore, an intelligent device for applying periodic pressure to cells is needed to solve the above problems. Summary of the invention

[0006] In order to solve the above problem, that is, to solve the problem that cells are easily exposed to the external environment during the process of being transported to the device for applying pressure, the present invention provides an intelligent device for applying periodic pressure to cells.

[0007] An intelligent device for applying periodic pressure to cells comprises a pressure mechanism, wherein the pressure mechanism comprises a base, a dust cover is arranged on the base, a detection component is arranged on the base, a transfer mechanism is arranged on the dust cover, the transfer mechanism comprises a transfer hole opened on the dust cover, a transfer tube is fixedly connected in the transfer hole, the transfer tube extends upward out of the dust cover, a closing cover is threadedly connected to the top of the transfer tube, a transfer box is arranged on the inner wall of the dust cover, a connecting tube is arranged at the top of the transfer box, and the connecting tube can be connected to the transfer hole.

[0008] Specifically, when in use, the closing cap is unscrewed from the transfer tube, and then the cell preservation device is connected to the connecting tube, and then the connecting tube is connected to the transfer tube, and then the cells are transported to the transfer box through the connecting tube and the transfer tube. After the cell transport is completed, the connecting tube and the transfer tube are disconnected, and then the closing cap is screwed back on the transfer tube, and then the cells in the transfer box are transported to the detection piece, and the detection piece applies pressure test to the cells.

[0009] By setting up the transfer tube, cells can be directly transported from the storage device to the dust cover, reducing the exposure of cells to the external environment during the transfer process, avoiding the influence of the external environment on the cell state, and after the cell transfer is completed, the transfer tube can be closed by the closing cover to avoid external gas entering the dust cover through the transfer tube during the test and affecting the cell test, and by setting up the transfer box, cells transferred into the dust cover can be stored, which is convenient for the subsequent dispersion of cells in the test piece.

[0010] Preferably, the transfer mechanism also includes a storage component arranged in the transfer box, the storage component includes a first motor fixedly connected to the bottom wall of the transfer box, the output end of the first motor is fixedly connected to the storage box, four storage cavities with openings upward are evenly opened in the storage box along the circumferential direction, each of the storage cavities is provided with a discharge hole with an opening downward, and each of the discharge holes is provided with an electric opening and closing valve.

[0011] Preferably, the transfer mechanism also includes a discharge member, which includes a second motor fixedly connected to the bottom wall of the transfer box, a driving gear fixedly sleeved on the second motor, a bent discharge pipe is provided through the bottom wall of the transfer box, a driven gear is fixedly connected to the discharge pipe, the driven gear is meshed with the driving gear, and the discharge pipe can be connected to the discharge hole.

[0012] Preferably, the transfer mechanism further includes a moving part, which includes an annular electromagnetic slide rail fixedly connected to the top wall of the dust cover, an electromagnetic slider being slidably connected to the electromagnetic slide rail, and the electromagnetic slider is fixedly connected to the transfer box.

[0013] Preferably, the moving part also includes a rod sleeve fixedly connected to the top surface of the transfer box, an abutment rod is slidably connected in the rod sleeve, a spring is connected between the abutment rod and the transfer box, the top end of the abutment rod is a hemispherical surface, twenty abutment blocks are evenly and fixedly connected to the top wall of the dust cover along the circumferential direction, the bottom surface of the abutment block is a hemispherical surface, and the abutment rod can abut against the abutment block.

[0014] Preferably, the detection element comprises a placement table, a piezoelectric ceramic module is arranged in the placement table, a culture dish is arranged on the placement table, and a pressure applying module is arranged on the top wall of the dust cover.

[0015] Preferably, four cavities opening upward are evenly opened in the culture dish along the circumferential direction, and the pressure applying module includes four pressure heads evenly arranged along the circumferential direction, and the pressure heads can enter the cavities.

[0016] Preferably, the dust cover is made of a transparent material, a scraper rod is fixedly connected to the transfer box, and the scraper rod is in contact with the inner wall of the dust cover.

[0017] The beneficial effects of the present invention are:

[0018] 1. Through the setting of the transfer tube, the cells can be directly transported from the storage device to the dust cover, which reduces the exposure of the cells to the external environment during the transfer process and avoids the influence of the external environment on the cell state. After the cell transfer is completed, the transfer tube can be closed by the closing cover to avoid external gas entering the dust cover through the transfer tube during the test and affecting the cell test. In addition, through the setting of the transfer box, the cells transferred into the dust cover can be stored, which is convenient for the subsequent dispersion of the cells in the test piece.

[0019] 2. Through the setting of the storage parts, the cells transported into the transfer box can be separated, and through the setting of the electric opening and closing valves, the cells in the four storage chambers can be discharged in turn, which is convenient for pressure observation of different cells.

[0020] 3. Through the setting of the discharge piece, the cells can be discharged from the storage cavity into the detection piece in sequence, which is convenient for the pressure observation of different cells. Through the setting of the moving part, the transfer box can perform circular motion, and at the same time cooperate with the discharge tube to evenly distribute the cells in the detection piece, so that the detection result is more accurate. Through the setting of the abutment rod and the abutment block, the transfer box can vibrate during the rotation, thereby driving the discharge tube to vibrate, making it easier for the cells to be discharged from the discharge tube. Through the setting of the scraping rod, the inner wall of the dust cover can be scraped when the transfer box rotates, which is convenient for the user to observe the inside of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0022] Figure 2 It is a front view of the present invention;

[0023] Figure 3 For the present invention Figure 2 Isometric section view at AA;

[0024] Figure 4 For the present invention Figure 3 A partial enlarged view of point B in the middle;

[0025] Figure 5 It is a right side view of the present invention;

[0026] Figure 6 For the present invention Figure 5 Isometric section view at mid-CC;

[0027] Figure 7 For the present invention Figure 6 A partial enlarged view of point E in the middle;

[0028] Figure 8 For the present invention Figure 5 Isometric section view at mid DD;

[0029] Fig. 9 It is a schematic diagram of the three-dimensional structure of the culture dish in the present invention;

[0030] Fig.10 It is a schematic diagram of the three-dimensional structure of the pressure head in the present invention.

[0031] In the figure:

[0032] 1. Pressure applying mechanism; 11. Base; 12. Dust cover; 13. Detection piece; 131. Placement table; 132. Piezoelectric ceramic module; 133. Culture dish; 134. Pressure applying module;

[0033] 2. Transfer mechanism; 21. Transfer hole; 22. Closing cover; 23. Transfer box; 24. Connecting pipe; 25. Storage member; 251. First motor; 252. Storage box; 253. Storage chamber; 254. Discharge hole; 255. Electric opening and closing valve; 26. Discharge member; 261. Second motor; 262. Driving gear; 263. Discharge pipe; 264. Driven gear; 27. Moving member; 271. Electromagnetic slide rail; 272. Electromagnetic slider; 273. Rod sleeve; 274. Abutment rod; 275. Spring; 276. Abutment block; 28. Wiping rod. DETAILED DESCRIPTION

[0034] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0035] like Figure 1 , 2As shown in Figures 3, 4 and 8, an embodiment of the present invention discloses an intelligent device for applying periodic pressure to cells, comprising a pressure mechanism 1, wherein the pressure mechanism 1 comprises a base 11, wherein a dust cover 12 is provided on the base 11, wherein a detection member 13 is provided on the base 11, wherein a transfer mechanism 2 is provided on the dust cover 12, wherein the transfer mechanism 2 comprises a transfer hole 21 provided on the dust cover 12, wherein a transfer tube is fixedly connected to the transfer hole 21, wherein the transfer tube extends upward out of the dust cover 12, wherein a closing cover 22 is threadedly connected to the top end of the transfer tube, wherein a transfer box 23 is provided on the inner wall of the dust cover 12, wherein a connecting tube 24 is provided at the top end of the transfer box 23, and wherein the connecting tube 24 can be connected to the transfer hole 21.

[0036] Specifically, when in use, the closing cover 22 is unscrewed from the transfer tube, and then the cell preservation device is connected to the connecting tube, and then the connecting tube is connected to the transfer tube, and then the cells and the preservation nutrient solution are transported to the transfer box 23 through the connecting tube and the transfer tube. After the cell transport is completed, the connecting tube and the transfer tube are disconnected, and then the closing cover 22 is screwed onto the transfer tube again, and then the cells in the transfer box 23 are transported to the detection part 13, and the detection part 13 applies pressure test to the cells.

[0037] By setting up the transfer tube, the cells can be directly transported from the storage device to the dust cover 12, thereby reducing the exposure of the cells to the external environment during the transfer process and avoiding the influence of the external environment on the cell state. After the cell transfer is completed, the transfer tube can be closed by the closing cover 22 to avoid external gas entering the dust cover 12 through the transfer tube during the test and affecting the cell test. By setting up the transfer box 23, the cells transferred into the dust cover 12 can be stored, which facilitates the subsequent dispersion of the cells in the detection piece 13.

[0038] like Figure 3 , 4 As shown in Figures 7 and 8, the transfer mechanism 2 also includes a storage component 25 arranged in the transfer box 23, and the storage component 25 includes a first motor 251 fixedly connected to the bottom wall of the transfer box 23, and the output end of the first motor 251 is fixedly connected to a storage box 252, and four storage cavities 253 with openings upward are evenly opened in the storage box 252 along the circumferential direction, and each of the storage cavities 253 is provided with a discharge hole 254 with an opening downward, and each of the discharge holes 254 is provided with an electric opening and closing valve 255.

[0039] Specifically, during use, cells enter the transfer box 23 from the transfer tube. When the cells enter one of the storage chambers 253, the first motor 251 is started, and the output end of the first motor 251 rotates 90°. The output end of the first motor 251 drives the storage box 252 to rotate. Then, new cells enter the new storage chamber 253 from the transfer tube. After the four storage chambers 253 are filled with cells, the transportation of cells to the transfer box 23 is stopped, and then the electric opening and closing valves 255 connecting the four storage chambers 253 are opened in turn, and the cells contained in the four storage chambers 253 are discharged from the electric opening and closing valves 255 respectively.

[0040] By setting the storage part 25, the cells transported into the transfer box 23 can be separated, and by setting the electric opening and closing valve 255, the cells in the four storage chambers 253 can be discharged in turn, which is convenient for pressure observation of different cells.

[0041] Furthermore, the electric on-off valve 255 is a prior art and will not be described in detail.

[0042] like Figure 3 , 4 As shown in Figure 7, the transfer mechanism 2 also includes a discharge member 26, which includes a second motor 261 fixedly connected to the bottom wall of the transfer box 23, and a driving gear 262 is fixedly sleeved on the second motor 261. A bent discharge pipe 263 is penetrated through the bottom wall of the transfer box 23, and a driven gear 264 is fixedly connected to the discharge pipe 263. The driven gear 264 is meshed with the driving gear 262, and the discharge pipe 263 can be connected to the discharge hole 254.

[0043] Specifically, when it is necessary to transport cells to the detection member 13 during use, the second motor 261 is started, and the output end of the second motor 261 drives the driving gear 262 to rotate, and the driving gear 262 drives the driven gear 264 to rotate, and the driven gear 264 drives the discharge pipe 263 to rotate, so that the lower end of the discharge pipe 263 moves to the upper side of the detection member 13, and then the first motor 251 is started, and the second motor 251 drives the storage chamber 253 to rotate, so that the upper end of the discharge pipe 263 is connected with the discharge hole 254, and then the electric opening and closing valve 255 is opened, so that the cells in the storage chamber 253 enter the discharge pipe 263, and then enter the detection member 13 from the discharge pipe 263, and then the first motor 251 continues to rotate, and the first motor 251 drives the storage chamber 253 to rotate, so that another discharge hole 254 is connected with the discharge pipe 263, so that other cells can be discharged into the detection member 13.

[0044] By disposing the discharge member 26, the cells can be discharged from the storage chamber 253 into the detection member 13 in sequence, so as to facilitate the pressure observation of different cells.

[0045] like Figure 3 , 4 As shown in , 6 and 8, the transfer mechanism 2 also includes a moving part 27, and the moving part 27 includes an annular electromagnetic slide rail 271 fixedly connected to the top wall of the dust cover 12, and an electromagnetic slider 272 is slidably connected in the electromagnetic slide rail 271, and the electromagnetic slider 272 is fixedly connected to the transfer box 23.

[0046] Specifically, when in use, the electromagnetic slider 272 is started, and the electromagnetic slider 272 moves in the electromagnetic slide rail 271 , and the electromagnetic slider 272 drives the transfer box 23 to move, and cooperates with the discharge tube 263 to evenly distribute the cells in the detection member 13 .

[0047] By setting the moving part 27, the transfer box 23 can perform circular motion, and at the same time cooperate with the discharge pipe 263 to evenly distribute the cells in the detection part 13, so that the detection result is more accurate.

[0048] Furthermore, the electromagnetic slide rail 271 and the electromagnetic slider 272 are prior art and will not be described in detail.

[0049] like Figure 4 , 8 As shown, the moving part 27 also includes a rod sleeve 273 fixedly connected to the top surface of the transfer box 23, and an abutment rod 274 is slidably connected in the rod sleeve 273. A spring 275 is connected between the abutment rod 274 and the transfer box 23. The top end of the abutment rod 274 is a hemispherical surface, and twenty abutment blocks 276 are evenly and fixedly connected to the top wall of the dust cover 12 along the circumferential direction. The bottom surface of the abutment block 276 is a hemispherical surface, and the abutment rod 274 can abut against the abutment block 276.

[0050] Specifically, during use, when the transfer box 23 rotates, the transfer box 23 drives the rod sleeve 273 to rotate, and the rod sleeve 273 drives the abutment rod 274 to rotate, and the abutment rod 274 continuously abuts against the abutment block 276, causing the transfer box 23 to vibrate.

[0051] By setting the abutment rod 274 and the abutment block 276 , the transfer box 23 can vibrate during the rotation process, thereby driving the discharge tube 263 to vibrate, making it easier for cells to be discharged from the discharge tube 263 .

[0052] like Figure 3 As shown, the detection member 13 includes a placement table 131 , a piezoelectric ceramic module 132 is disposed in the placement table 131 , a culture dish 133 is disposed on the placement table 131 , and a pressure applying module 134 is disposed on the top wall of the dust cover 12 .

[0053] Specifically, during use, after the cells enter the culture dish 133, the pressure applying module 134 is started, the pressure head of the pressure applying module 134 enters the culture dish 133, and then power is supplied to the piezoelectric ceramic module 132, the piezoelectric ceramic module 132 generates axial vibration, and applies periodic pressure to the bottom of the culture dish 133.

[0054] Furthermore, the detection element 13 is a prior art and will not be described in detail.

[0055] like Fig. 9 , 10 As shown, four cavities opening upward are evenly opened in the culture dish 133 along the circumferential direction, and the pressure applying module 134 includes four pressure heads evenly arranged along the circumferential direction, and the pressure heads can enter the cavities.

[0056] Specifically, the four pressure heads enter into the four cavities respectively and apply pressure to the four cavities respectively.

[0057] like Figure 3 , 4 As shown, the dust cover 12 is made of a transparent material, and a scraper rod 28 is fixedly connected to the transfer box 23 , and the scraper rod 28 is in contact with the inner wall of the dust cover 12 .

[0058] Specifically, during use, when the transfer box 23 rotates, the transfer box 23 drives the scraping rod 28 to rotate, and the scraping rod 28 scrapes the inner wall of the transfer box 23.

[0059] By providing the scraping rod 28 , the inner wall of the dust cover 12 can be scraped when the transfer box 23 rotates, so that the user can observe the inside of the device conveniently.

[0060] It should be noted that in the description of the present invention, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings, which are only for the convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0061] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] The term "comprise" or any other similar term is intended to cover a non-exclusive inclusion, such that a process, article, or apparatus / device that includes a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, article, or apparatus / device.

[0063] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. An intelligent device for applying periodic pressure to cells, characterized in that: The invention comprises a pressure-applying mechanism (1), wherein the pressure-applying mechanism (1) comprises a base (11), a dust cover (12) is arranged on the base (11), a detection component (13) is arranged on the base (11), a transfer mechanism (2) is arranged on the dust cover (12), the transfer mechanism (2) comprises a transfer hole (21) provided on the dust cover (12), a transfer pipe is fixedly connected in the transfer hole (21), the transfer pipe extends upward out of the dust cover (12), a closing cover (22) is threadedly connected to the top end of the transfer pipe, a transfer box (23) is arranged on the inner wall of the dust cover (12), a connecting pipe (24) is arranged at the top end of the transfer box (23), and the connecting pipe (24) can be connected to the transfer hole (21).

2. The intelligent device for applying periodic pressure to cells according to claim 1, characterized in that: The transfer mechanism (2) further comprises a storage component (25) arranged in the transfer box (23), the storage component (25) comprising a first motor (251) fixedly connected to the bottom wall of the transfer box (23), the output end of the first motor (251) being fixedly connected to a storage box (252), the storage box (252) being provided with four storage cavities (253) opening upward evenly along a circumferential direction, each of the storage cavities (253) being provided with a discharge hole (254) opening downward, and each of the discharge holes (254) being provided with an electric opening and closing valve (255).

3. The intelligent device for applying periodic pressure to cells according to claim 2, characterized in that: The transfer mechanism (2) further comprises a discharge member (26), the discharge member (26) comprising a second motor (261) fixedly connected to the bottom wall of the transfer box (23), a driving gear (262) fixedly sleeved on the second motor (261), a bent discharge pipe (263) penetrating the bottom wall of the transfer box (23), a driven gear (264) fixedly connected to the discharge pipe (263), the driven gear (264) meshing with the driving gear (262), and the discharge pipe (263) capable of communicating with the discharge hole (254).

4. The intelligent device for applying periodic pressure to cells according to claim 3, characterized in that: The transfer mechanism (2) also includes a moving part (27), and the moving part (27) includes an annular electromagnetic slide rail (271) fixedly connected to the top wall of the dust cover (12), and an electromagnetic slider (272) is slidably connected in the electromagnetic slide rail (271), and the electromagnetic slider (272) is fixedly connected to the transfer box (23).

5. The intelligent device for applying periodic pressure to cells according to claim 4, characterized in that: The moving part (27) further comprises a rod sleeve (273) fixedly connected to the top surface of the transfer box (23); an abutment rod (274) is slidably connected in the rod sleeve (273); a spring (275) is connected between the abutment rod (274) and the transfer box (23); the top end of the abutment rod (274) is a hemispherical surface; twenty abutment blocks (276) are evenly and fixedly connected to the top wall of the dust cover (12) along the circumferential direction; the bottom surface of the abutment block (276) is a hemispherical surface; and the abutment rod (274) can abut against the abutment block (276).

6. The intelligent device for applying periodic pressure to cells according to claim 5, characterized in that: The detection element (13) comprises a placement table (131), a piezoelectric ceramic module (132) is arranged in the placement table (131), a culture dish (133) is arranged on the placement table (131), and a pressure applying module (134) is arranged on the top wall of the dust cover (12).

7. The intelligent device for applying periodic pressure to cells according to claim 6, characterized in that: The culture dish (133) is provided with four cavities evenly opened upward in a circumferential direction, and the pressure applying module (134) comprises four pressure heads evenly arranged in a circumferential direction, and the pressure heads are capable of entering the cavities.

8. The intelligent device for applying periodic pressure to cells according to claim 7, characterized in that: The dust cover (12) is made of a transparent material, and a scraper rod (28) is fixedly connected to the transfer box (23), and the scraper rod (28) is in contact with the inner wall of the dust cover (12).

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