Method for controlling bacterial pollution in microalgae culture and culture device thereof

Abstract

The invention discloses a control method for bacterial pollution in microalgae culture and a culture device thereof, and relates to the field of microalgae culture, and the control method comprises the following steps: step 1, carbon dioxide supply: when microalgae are cultured in a transparent incubator, when a carbon dioxide sensor detects that carbon dioxide in the incubator is smaller than a preset interval, the carbon dioxide sensor detects that carbon dioxide in the incubator is smaller than the preset interval; the valve is opened, so that the carbon dioxide enters the air supply pipeline through the communicating pipe, the circular reducer pipe and the connecting pipe, and then is sprayed into the nutrient solution from the spray head; 2, carbon dioxide is dissolved, when the carbon dioxide passes through a round reducer pipe, a driving assembly in a treatment mechanism is driven, namely, an impeller rotates, the impeller transmits rotating force to a third straight gear, the rotating third straight gear drives a gear ring in a stirring assembly to rotate, and the carbon dioxide is stirred; a gear ring drives a stirring rod to stir a nutrient solution by controlling a horizontal plate to cooperate with sprayed bubbles, so that the dissolution of carbon dioxide is accelerated, the growth conditions of microalgae are optimized, and the growth of bacteria is inhibited; and step 3, cleaning the inner wall of the box: when a horizontal plate moves, pushing a pushing block in contact with the horizontal plate to move through a first sliding block, and pushing a second sliding block by the pushing block to enable a scraping plate to move along the inner wall of the transparent culture box, so as to clean the attached microalgae and ensure the light transmittance. According to the device, the stirring rod can be driven through the impeller and the gear system while carbon dioxide is provided, the sprayed carbon dioxide is promoted to be dissolved in a nutrient solution, the growth conditions of microalgae are optimized, the growth of bacteria is inhibited, manual intervention is reduced, and the culture efficiency and consistency are improved.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of microalgae cultivation, and in particular to a method for controlling bacterial contamination in microalgae cultivation and a cultivation device thereof. Background Art

[0002] Microalgae refers to algae groups that are tiny, between microorganisms and plants, and can carry out oxygenic photosynthesis. Through photosynthesis, microalgae cells can effectively utilize light energy to convert H2O, CO2 and inorganic salts into organic matter and release oxygen, thereby creating conditions for the survival of other organisms. In this sense, microalgae are an important source of life on Earth. Common contaminating organisms in the cultivation process of microalgae include zooplankton, bacteria, other microalgae and viruses. These contaminating organisms will affect the growth and yield of microalgae, so it is critical to prevent pollution during production.

[0003] An existing microalgae cultivation device and microalgae cultivation method include a transparent tank body, the diameter of which gradually increases from top to bottom, and the top of the tank body is designed to be open. A discharge pipe is connected to the center of the bottom of the tank body, and the diameter of the discharge pipe gradually increases from top to bottom. A tank cover is provided on the top of the tank body, and a transparent lampshade is rotatably inserted through the center of the top of the tank cover.

[0004] Although the "air ring" formed by the above technology prevents microalgae and impurities from adhering to the inner wall of the tank and ensures that external light can better irradiate the tank, manual intervention is required during operation, such as cleaning microalgae, which may reduce the convenience of operation and the consistency of the cultivation process. In addition, natural convection is less efficient in dissolving carbon dioxide, which will reduce the photosynthesis efficiency of microalgae and lead to increased bacterial contamination. Summary of the invention

[0005] Based on this, the purpose of the present invention is to provide a method for controlling bacterial contamination in microalgae culture and a culture device thereof, so as to solve the technical problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method for controlling bacterial contamination in microalgae culture comprises the following steps:

[0008] Step 1: Supplying carbon dioxide. When the microalgae are cultured in a transparent culture box, when the carbon dioxide sensor detects that the carbon dioxide in the box is less than a preset range, the valve is opened to allow the carbon dioxide to enter the gas supply pipeline through the connecting pipe, the circular reducer and the connecting pipe, and then sprayed from the nozzle into the nutrient solution;

[0009] Step 2: Carbon dioxide dissolves. When carbon dioxide passes through the circular reducer, the driving component in the processing mechanism is driven, that is, the impeller rotates, and the impeller transmits the rotational force to the third spur gear. The rotating third spur gear drives the gear ring in the stirring component to rotate. The gear ring drives the stirring rod to stir the nutrient solution by controlling the horizontal plate to cooperate with the ejected bubbles, thereby accelerating the dissolution of carbon dioxide, optimizing the growth conditions of microalgae and inhibiting bacterial growth.

[0010] Step 3: Clean the inner wall of the box. When the horizontal plate moves, the first slider will push the contacting push block to move. The push block pushes the second slider to move the scraper along the inner wall of the transparent culture box to clean the attached microalgae and ensure light transmittance.

[0011] According to the above technical scheme, a culture device for controlling bacterial contamination in microalgae culture is also provided, wherein an annular groove is provided on the top of the outer wall of the transparent culture box, an annular air supply pipeline is provided on the bottom of the transparent culture box, a plurality of nozzles are provided on the bottom wall of the air supply pipeline, a connecting pipe is threadedly installed on the outer wall of the air supply pipeline, the connecting pipe penetrates the wall of the transparent culture box and is located outside, the connecting pipe is connected to the connecting pipe through a circular reducer, and a through groove is provided on one side of the outer wall of the circular reducer;

[0012] The processing mechanism is composed of a driving component and a stirring component. The driving component includes an impeller. One side of the impeller passes through a through slot and is located in a circular reducer. A shaft is passed through the center of the impeller. A first spur gear is installed on the top of the shaft. The third spur gear is connected to a second spur gear through a transmission shaft. The second spur gear is meshed with the first spur gear.

[0013] The stirring assembly includes a gear ring, a plurality of stirring rods and two top plates. The inner ring of the gear ring is slidably set in an annular groove. The tooth surface of the gear ring is meshed with the tooth surface of the third spur gear. The upper surface of the gear ring is located on the wall outside the annular groove and a plurality of vertical plates are evenly fixed thereon. The top ends of the plurality of vertical plates are fixed with horizontal plates. The plurality of stirring rods are located in a transparent incubator and the top ends are fixed with bolts to the lower surfaces of the horizontal plates.

[0014] The technical solution is specific in that a protective box is installed at the through groove on the outer wall of the circular reducer by screws, a protective shell is installed on the upper surface of the protective box by screws, the bottom end of the shaft is rotatably connected to the inner bottom wall of the protective box, the top end of the shaft passes through the top wall of the protective box and extends into the protective shell, a horizontal partition is fixed inside the protective shell, the top end of the shaft is rotatably connected to the lower surface of the partition, the transmission shaft passes through the partition and is rotatably connected to the contact portion, and the top end of the transmission shaft is rotatably connected to the inner top wall of the protective shell.

[0015] Specifically, the third spur gear and the second spur gear are fixedly mounted on the top and bottom of the transmission shaft respectively, the diameter of the first spur gear is the same as the diameter of the third spur gear, and the diameter of the second spur gear is smaller than the diameter of the first spur gear.

[0016] Specifically, the gear ring is located on the upper and lower surfaces of the slide groove and ball bearings are evenly embedded thereon, a slide groove is provided on the top wall of the transparent incubator, and a first sliding block is fixed to one side of the lower surface of the plurality of horizontal plates close to the vertical plate, and the plurality of first sliding blocks are slidably connected to the slide groove.

[0017] Specifically, the lower surfaces of the two top plates are fixed with second sliders, the two second sliders are slidably connected to the slide groove, push blocks are slidably provided on both sides of the second slider in the slide groove, one end of each push block away from the second slider is in contact with the outer wall of the first slider, the width of the two top plates is greater than the wall thickness of the transparent incubator, scrapers are symmetrically provided on the inner wall of the transparent incubator, and the top ends of the two scrapers are fixedly connected to the lower surface of the top plate.

[0018] Specifically, the technical solution of the present invention is provided with a ring in the center between the two top plates, horizontal connecting rods are fixed on both sides of the outer wall of the ring, the ends of the two connecting rods are fixedly connected to the outer walls of the top plates, a vertical rod is passed through the ring, the bottom end of the vertical rod is located at the center of the air supply pipe, four support plates are evenly sleeved on the air supply pipe, and the outer walls of the four support plates are connected to the bottom outer wall of the vertical rod through a fixing rod.

[0019] Specifically, the transparent incubator is provided with transparent covers symmetrically on the top, the bottoms of the two transparent covers are both located below the annular groove, and one of the transparent covers is located on the wall of the protective shell and has a groove.

[0020] Specifically, the inner top walls of the two transparent covers are symmetrically mounted with fluorescent lamps, and the inner top wall of one of the transparent covers is mounted with a carbon dioxide sensor on one side of the fluorescent lamp.

[0021] Specifically, a base is fixed to the bottom of the transparent incubator, the diameter of the base is the same as the diameter of the transparent incubator, three installation grooves are symmetrically opened on both sides of the outer wall of the base, a telescopic plate is installed in each of the installation grooves, the telescopic end of each of the telescopic plates is located outside the installation groove, three side panels are evenly fixed to the outer walls of the two transparent covers, the bottom end of each of the side panels is fixed to the upper surface of the telescopic end of the telescopic plate by screws, and a ball bearing is embedded on the lower surface of the telescopic end of each of the telescopic plates.

[0022] In summary, the present invention mainly has the following beneficial effects: First, the carbon dioxide sensor detects the carbon dioxide content in the box in real time. When it is less than the preset range, the carbon dioxide supply is started, and the carbon dioxide enters the gas supply pipeline through the connecting pipe, the circular reducer and the connecting pipe, and enters and exits the nutrient solution from the nozzle. When the gas is supplied, the impeller is driven to rotate, and the stirring rod is driven to move slowly through the gear system, so as to promote the dissolution of the ejected carbon dioxide in the nutrient solution, optimize the growth conditions of microalgae, inhibit the growth of bacteria, reduce manual intervention, and improve the culture efficiency and consistency;

[0023] During the stirring process, the top plate and scraper are driven to move by the push block, and the scraper automatically cleans the inner wall of the box to remove the attached microalgae to maintain the light transmittance without providing an additional power source. After opening the transparent cover, the scraper can be taken out of the box, making it convenient for the staff to clean and maintain it. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a diagram of the method steps of the present invention;

[0025] Figure 2 is a schematic diagram of a culture device of the present invention;

[0026] Figure 3 For the present invention Figure 2 Top view;

[0027] Figure 4 It is a schematic diagram of the transparent incubator of the present invention;

[0028] Figure 5 For the present invention Figure 4 Top view;

[0029] Figure 6 It is a schematic diagram of the processing mechanism of the present invention;

[0030] Figure 7 It is a structural diagram of the stirring assembly of the present invention;

[0031] Figure 8 It is a schematic diagram of the scraper structure of the present invention.

[0032] Description of the drawings: 1. Transparent culture box; 101. Base; 1011. Mounting groove; 102. Slide groove; 103. Ring groove; 2. Air supply pipe; 201. Nozzle; 202. Support plate; 203. Connecting pipe; 204. Circular reducer; 204. Through groove; 205. Connecting pipe; 206. Fixed rod; 207. Vertical rod; 3. Protective box; 301. Protective shell; 3011. Partition; 4. Processing mechanism; 5. Driving assembly; 501. Shaft; 502. Impeller ; 503, first spur gear; 504, transmission shaft; 505, second spur gear; 506, third spur gear; 6, stirring assembly; 601, gear ring; 602, vertical plate; 603, horizontal plate; 6031, stirring rod; 6032, first slider; 604, push block; 605, second slider; 606, top plate; 6061, scraper; 607, connecting rod; 608, sleeve ring; 7, transparent cover; 701, side plate; 702, telescopic plate; 703, accommodating groove. DETAILED DESCRIPTION

[0033] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0034] The following describes an embodiment of the present invention based on its overall structure.

[0035] Example

[0036] See also Figure 1-8 As shown, a method for controlling bacterial contamination in microalgae culture comprises the following steps:

[0037] Step 1: Supplying carbon dioxide. When the microalgae are cultured in the transparent culture box 1, when the carbon dioxide sensor detects that the carbon dioxide in the box is less than a preset range, the valve is opened to allow the carbon dioxide to enter the gas supply pipe 2 through the connecting pipe 205, the circular reducer 204 and the connecting pipe 203, and then sprayed from the nozzle 201 into the nutrient solution;

[0038] Step 2: Carbon dioxide dissolves. When carbon dioxide passes through the circular reducer 204, the driving assembly 5 in the processing mechanism 4 is driven, that is, the impeller 502 rotates, and the impeller 502 transmits the rotational force to the third spur gear 506. The rotating third spur gear 506 drives the gear ring 601 in the stirring assembly 6 to rotate. The gear ring 601 drives the stirring rod 6031 to stir the nutrient solution by controlling the horizontal plate 603 to cooperate with the ejected bubbles, thereby accelerating the dissolution of carbon dioxide, optimizing the growth conditions of microalgae and inhibiting the growth of bacteria.

[0039] Step 3: Cleaning the inner wall of the box. When the horizontal plate 603 moves, the first slider 6032 pushes the contacting push block 604 to move. The push block 604 pushes the second slider 605 to move the scraper 6061 along the inner wall of the transparent culture box 1 to clean the attached microalgae and ensure the light transmittance.

[0040] The connecting pipe 205 in the present application is connected to the carbon dioxide supply equipment, the carbon dioxide sensor adopts the GS-Y5 model, and all electrical components are controlled by the control chip in the external controller, and the structures in the processing mechanism 4 are made of lightweight materials, such as polycarbonate (PC) board and polymethyl methacrylate (PMMA) board, which have good light transmittance.

[0041] See also Figure 2-7 As shown, according to the above embodiments, a culture device for controlling bacterial contamination in microalgae culture will also be provided, an annular groove 103 is provided on the top of the outer wall of the transparent culture box 1, an annular air supply pipe 2 is provided on the bottom of the transparent culture box 1, a plurality of nozzles 201 are provided on the bottom wall of the air supply pipe 2, a connecting pipe 203 is threadedly installed on the outer wall of the air supply pipe 2, the connecting pipe 203 passes through the wall of the transparent culture box 1 and is located outside, the connecting pipe 203 is connected to the connecting pipe 205 through a circular reducer 204, and a through groove 2041 is provided on one side of the outer wall of the circular reducer 204; wherein the outer wall on one side of the bottom of the transparent culture box 1 is connected with a discharge pipe.

[0042] The processing mechanism 4 is composed of a driving component 5 and a stirring component 6. The driving component 5 includes an impeller 502. One side of the impeller 502 passes through the through slot 2041 and is located in the circular reducer 204. A shaft 501 is passed through the center of the impeller 502. A first spur gear 503 is installed on the top of the shaft 501. The third spur gear 506 is connected to the second spur gear 505 through the transmission shaft 504. The second spur gear 505 is meshed with the first spur gear 503.

[0043] The stirring assembly 6 includes a gear ring 601, a plurality of stirring rods 6031 and two top plates 606. The inner ring of the gear ring 601 is slidably set in the annular groove 103. The tooth surface of the gear ring 601 is meshed with the tooth surface of the third spur gear 506. The upper surface of the gear ring 601 is located on the wall outside the annular groove 103, and a plurality of vertical plates 602 are evenly fixed thereon. The top ends of the plurality of vertical plates 602 are all fixed with horizontal plates 603. The plurality of stirring rods 6031 are all located in the transparent incubator 1 and the top ends are bolted to the lower surface of the horizontal plate 603.

[0044] When the microalgae are cultivated in the transparent culture box 1, when the carbon dioxide sensor detects that the carbon dioxide concentration in the box is less than the preset range, the valve of the carbon dioxide supply device will be opened, so that the carbon dioxide enters the gas supply pipe 2 through the connecting pipe 205, the circular reducer 204 and the connecting pipe 203, and then is sprayed from the nozzle 201 into the nutrient solution. When the carbon dioxide passes through the circular reducer 204, the gas velocity will decrease due to the change in the pipe diameter, and then it may increase again, which can optimize the gas velocity reduction effect, thereby better controlling the supply of carbon dioxide. When the impeller 502 is rotated, the impeller 502 rotates and drives the first spur gear 503 to rotate through the shaft 501, and the first spur gear 503 drives the meshed second spur gear 505 to rotate, and the second spur gear 505 drives the third spur gear 506 to rotate through the transmission shaft 504, and the third spur gear 506 drives the meshed gear ring 601 to rotate along the ring groove 103, and the gear ring 601 drives the horizontal plate 603 to move through the multiple vertical plates 602, and the multiple horizontal plates 603 drive the stirring rod 6031 to move, so as to promote the dissolution of the ejected carbon dioxide in the nutrient solution, and stir the microalgae to increase the contact with the carbon dioxide;

[0045] Therefore, the present application can drive the stirring rod 6031 to move slowly through the impeller 502 and the gear system, promote the dissolution of the ejected carbon dioxide in the nutrient solution, optimize the growth conditions of microalgae, inhibit bacterial growth, reduce manual intervention, and improve culture efficiency and consistency.

[0046] See also Figure 4-8 As shown, the outer wall of the circular reducer 204 is located at the through groove 2041 and a protective box 3 is installed by screws, and a protective shell 301 is installed on the upper surface of the protective box 3 by screws, the bottom end of the shaft rod 501 is rotatably connected to the inner bottom wall of the protective box 3, and the top end of the shaft rod 501 penetrates the top wall of the protective box 3 and extends into the protective shell 301, and a horizontal partition 3011 is fixed inside the protective shell 301, and the top end of the shaft rod 501 is rotatably connected to the lower surface of the partition 3011, the transmission shaft 504 penetrates the partition 3011 and is rotatably connected to the contact part, and the top end of the transmission shaft 504 is rotatably connected to the inner top wall of the protective shell 301, and the third spur gear 506 and the second spur gear 505 are respectively fixedly sleeved on the top and bottom of the transmission shaft 504, and the diameter of the first spur gear 503 is the same as the diameter of the third spur gear 506, and the diameter of the second spur gear 505 is smaller than the diameter of the first spur gear 503;

[0047] The gear ring 601 is evenly embedded with balls on the upper and lower surfaces of the slide groove 102. The top wall of the transparent incubator 1 is provided with a slide groove 102. The lower surfaces of the plurality of horizontal plates 603 are all fixed with first sliders 6032 on one side close to the vertical plates 602. The plurality of first sliders 6032 are all slidably connected with the slide groove 102.

[0048] The lower surfaces of the two top plates 606 are fixed with second sliders 605, and the two second sliders 605 are slidably connected with the slide groove 102. Push blocks 604 are slidably arranged on both sides of the second sliders 605 in the slide groove 102. One end of each push block 604 away from the second slider 605 contacts the outer wall of the first slider 6032. The width of the two top plates 606 is greater than the wall thickness of the transparent incubator 1. Scrapers 6061 are symmetrically arranged on the inner wall of the transparent incubator 1. The top ends of the two scrapers 6061 are in contact with the outer wall of the top plate 606. The lower surface is fixedly connected, and a ring 608 is provided in the center between the two top plates 606. Horizontal connecting rods 607 are fixed on both sides of the outer wall of the ring 608. The ends of the two connecting rods 607 are fixedly connected to the outer wall of the top plate 606. A vertical rod 207 is passed through the ring 608. The bottom end of the vertical rod 207 is located at the center of the air supply pipe 2. Four support plates 202 are evenly sleeved on the air supply pipe 2. The outer walls of the four support plates 202 are connected to the bottom outer wall of the vertical rod 207 through the fixing rod 206.

[0049] When the gear ring 601 is driven by the third spur gear 506, the friction with the ring groove 103 is reduced by the ball bearing to ensure the movement process. When the horizontal plate 603 follows the vertical plate 602 and the gear ring 601 to move, it will also drive the first slider 6032 to move along the slide groove 102. Part of the first slider 6032 will push the contacting push block 604 to move during the movement, and the push block 604 will push the second slider 605 to move. At this time, the second slider 605 will drive the top plate 606 to move, and the top plate 606 drives the scraper 6061 to move along the inner box wall of the transparent culture box 1 to clean up the attached microalgae. Since the horizontal plate 603 moves slowly, the damage to the microalgae caused by the scraper 6061 during cleaning can be reduced or avoided, so as to maintain the light transmittance without providing an additional power source.

[0050] When the two horizontal plates 603 move, the connecting rod 607 and the ring 608 are also driven to move, so that the ring 608 rotates along the vertical rod 207. When the transparent cover 7 moves to both sides to expose the top of the transparent incubator 1, the staff can pull up the ring 608 or the connecting rod 607 by hand to remove the top plate 606 and the scraper 6061 from the box for cleaning and maintenance.

[0051] See also Figure 2-4 As shown, transparent covers 7 are symmetrically provided on the top of the transparent incubator 1, the bottoms of the two transparent covers 7 are both located below the annular groove 103, one transparent cover 7 is located on the wall of the protective shell 301 and a groove is provided, fluorescent lamps are symmetrically installed on the inner top walls of the two transparent covers 7, and a carbon dioxide sensor is installed on the inner top wall of one transparent cover 7 on one side of the fluorescent lamp; the fluorescent lamp can provide light at night or in gloomy weather.

[0052] A base 101 is fixed to the bottom of the transparent incubator 1, and the diameter of the base 101 is the same as the diameter of the transparent incubator 1. Three installation grooves 1011 are symmetrically opened on both sides of the outer wall of the base 101, and a telescopic plate 702 is installed in each installation groove 1011. The telescopic end of each telescopic plate 702 is located outside the installation groove 1011. Three side panels 701 are evenly fixed to the outer walls of the two transparent covers 7. The bottom end of each side panel 701 is screwed to the upper surface of the telescopic end of the telescopic plate 702, and a ball bearing is embedded on the lower surface of the telescopic end of each telescopic plate 702.

[0053] When it is necessary to clean and maintain the scraper 6061 and the inside of the transparent incubator 1, the staff pushes the transparent cover 7 to both sides, and the two transparent covers 7 will mobilize the side panels 701 to move. The moving side panels 701 stretch the telescopic ends of the telescopic plates 702 connected to the bottom. Since the lower surface of the telescopic ends is provided with balls, it can not only play a supporting role but also reduce the friction with the ground to ensure smooth movement. At this time, the two transparent covers 7 are separated to cancel the covering seal of the transparent incubator 1, which is convenient for the staff to carry out cleaning and maintenance.

[0054] The working principle of the present invention is:

[0055] When the microalgae are cultivated in the transparent culture box 1, when the carbon dioxide sensor detects that the carbon dioxide concentration in the box is less than the preset range, the valve of the carbon dioxide supply device will be opened, so that the carbon dioxide enters the gas supply pipe 2 through the connecting pipe 205, the circular reducer 204 and the connecting pipe 203, and then is sprayed from the nozzle 201 into the nutrient solution. When the carbon dioxide passes through the circular reducer 204, the gas velocity will decrease due to the change in the pipe diameter, and then it may increase again, which can optimize the gas velocity reduction effect, thereby better controlling the supply of carbon dioxide. When the carbon dioxide passes through the circular reducer 204, The impeller 502 is driven to rotate, and the impeller 502 rotates to drive the first spur gear 503 to rotate through the shaft 501, and the first spur gear 503 drives the meshed second spur gear 505 to rotate, and the second spur gear 505 drives the third spur gear 506 to rotate through the transmission shaft 504, and the third spur gear 506 drives the meshed gear ring 601 to rotate along the ring groove 103, and the friction with the ring groove 103 is reduced by the ball bearing to ensure the movement process, and the gear ring 601 drives the horizontal plate 603 to move through the multiple vertical plates 602, and the multiple horizontal plates 603 drive the stirring rod 6031 to move, so as to promote the dissolution of the ejected carbon dioxide in the nutrient solution;

[0056] The multiple horizontal plates 603 will also drive the first slider 6032 to move along the slide groove 102. During the movement, part of the first slider 6032 will push the contacting push block 604 to move, and the push block 604 will push the second slider 605 to move. At this time, the second slider 605 will drive the top plate 606 to move, and the top plate 606 will drive the scraper 6061 to move along the inner box wall of the transparent culture box 1 to clean up the attached microalgae.

[0057] Although an embodiment of the present invention has been shown and described, this specific embodiment is merely an explanation of the present invention and is not a limitation of the invention. The specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions and variations to the embodiments without creative contributions as needed without departing from the principles and purpose of the present invention. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A method for controlling bacterial contamination in microalgae culture, characterized in that , including the following steps: Step 1: supplying carbon dioxide. When the microalgae are cultured in the transparent culture box (1), when the carbon dioxide sensor detects that the carbon dioxide in the box is less than a preset range, the valve is opened to allow the carbon dioxide to enter the gas supply pipe (2) through the connecting pipe (205), the circular reducer (204) and the connecting pipe (203), and then be sprayed from the nozzle (201) into the nutrient solution; Step 2: dissolving carbon dioxide. When carbon dioxide passes through the circular reducer (204), the driving assembly (5) in the processing mechanism (4) is driven, that is, the impeller (502) rotates. The impeller (502) transmits the rotational force to the third spur gear (506). The rotating third spur gear (506) drives the gear ring (601) in the stirring assembly (6) to rotate. The gear ring (601) drives the stirring rod (6031) to stir the nutrient solution by controlling the horizontal plate (603) to cooperate with the ejected bubbles, thereby accelerating the dissolution of carbon dioxide, optimizing the growth conditions of microalgae and inhibiting the growth of bacteria. Step 3: Cleaning the inner wall of the box. When the horizontal plate (603) moves, the first slider (6032) pushes the contacting push block (604) to move. The push block (604) pushes the second slider (605) to move the scraper (6061) along the inner wall of the transparent culture box (1), thereby cleaning the attached microalgae and ensuring the light transmittance.

2. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 1, characterized in that: The top of the outer wall of the transparent culture box (1) is provided with an annular groove (103); the bottom of the inner wall of the transparent culture box (1) is provided with an annular air supply pipe (2); the bottom wall of the air supply pipe (2) is provided with a plurality of nozzles (201); the outer wall of the air supply pipe (2) is threadedly mounted with a connecting pipe (203); the connecting pipe (203) penetrates the wall of the transparent culture box (1) and is located outside; the connecting pipe (203) is connected to the connecting pipe (205) via a circular reducer (204); a through groove (2041) is provided on one side of the outer wall of the circular reducer (204); The processing mechanism (4) is composed of a driving component (5) and a stirring component (6), wherein the driving component (5) comprises an impeller (502), one side of the impeller (502) passes through a through slot (2041) and is located in a circular reducer (204), a shaft (501) is passed through the center of the impeller (502), a first spur gear (503) is installed at the top end of the shaft (501), the third spur gear (506) is connected to a second spur gear (505) via a transmission shaft (504), and the second spur gear (505) is meshedly connected with the first spur gear (503); The stirring assembly (6) comprises a gear ring (601), a plurality of stirring rods (6031) and two top plates (606); the inner ring of the gear ring (601) is slidably arranged in the annular groove (103); the tooth surface of the gear ring (601) is meshedly connected with the tooth surface of the third spur gear (506); and the upper surface of the gear ring (601) is located on the wall outside the annular groove (103) and is evenly fixed with a plurality of vertical plates (602); the top ends of the plurality of vertical plates (602) are all fixed with horizontal plates (603); and the plurality of stirring rods (6031) are all located in the transparent culture box (1) and the top ends are fixed to the lower surface of the horizontal plates (603) by bolts.

3. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 2, characterized in that: The outer wall of the circular reducer (204) is located at the through groove (2041) and is installed with a protection box (3) by screws, and the upper surface of the protection box (3) is installed with a protection shell (301) by screws, the bottom end of the shaft rod (501) is rotatably connected to the inner bottom wall of the protection box (3), the top end of the shaft rod (501) passes through the top wall of the protection box (3) and extends into the protection shell (301), a horizontal partition (3011) is fixed inside the protection shell (301), the top end of the shaft rod (501) is rotatably connected to the lower surface of the partition (3011), the transmission shaft (504) passes through the partition (3011) and is rotatably connected to the contact part, and the top end of the transmission shaft (504) is rotatably connected to the inner top wall of the protection shell (301).

4. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 3, characterized in that: The third spur gear (506) and the second spur gear (505) are respectively fixedly sleeved on the top and bottom of the transmission shaft (504); the diameter of the first spur gear (503) is the same as the diameter of the third spur gear (506), and the diameter of the second spur gear (505) is smaller than the diameter of the first spur gear (503).

5. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 2, characterized in that: The gear ring (601) is evenly embedded with balls on the upper and lower surfaces of the slide groove (102); the top wall of the transparent culture box (1) is provided with a slide groove (102); the lower surfaces of the plurality of horizontal plates (603) are all fixed with first sliding blocks (6032) on one side close to the vertical plates (602); and the plurality of first sliding blocks (6032) are all slidably connected to the slide groove (102).

6. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 5, characterized in that: A second slider (605) is fixed to the lower surface of the two top plates (606), and the two second sliders (605) are slidably connected to the slide groove (102). Push blocks (604) are slidably provided on both sides of the second slider (605) in the slide groove (102), and one end of each push block (604) away from the second slider (605) contacts the outer wall of the first slider (6032). The width of the two top plates (606) is greater than the wall thickness of the transparent incubator (1), and scrapers (6061) are symmetrically provided on the inner wall of the transparent incubator (1), and the top ends of the two scrapers (6061) are fixedly connected to the lower surface of the top plate (606).

7. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 6, characterized in that: A collar (608) is provided in the center between the two top plates (606), and horizontal connecting rods (607) are fixed on both sides of the outer wall of the collar (608), and the ends of the two connecting rods (607) are fixedly connected to the outer wall of the top plate (606). A vertical rod (207) is passed through the collar (608), and the bottom end of the vertical rod (207) is located at the center of the air supply pipe (2). Four support plates (202) are evenly sleeved on the upper part of the air supply pipe (2), and the outer walls of the four support plates (202) are connected to the bottom outer wall of the vertical rod (207) through the fixing rod (206).

8. The culture device for controlling bacterial contamination in microalgae culture according to claim 2, characterized in that: A transparent cover (7) is symmetrically arranged on the top of the transparent culture box (1), the bottoms of the two transparent covers (7) are both located below the annular groove (103), and one of the transparent covers (7) is located on the wall of the protective shell (301) and is provided with a groove.

9. The culture device for controlling bacterial contamination in microalgae culture according to claim 8, characterized in that: Fluorescent lamps are symmetrically mounted on the inner top walls of the two transparent covers (7), and a carbon dioxide sensor is mounted on the inner top wall of one of the transparent covers (7) on one side of the fluorescent lamp.

10. The culture device of the method for controlling bacterial contamination in microalgae culture according to claim 8, characterized in that: A base (101) is fixed to the bottom of the transparent culture box (1), the diameter of the base (101) is the same as the diameter of the transparent culture box (1), three installation grooves (1011) are symmetrically opened on both sides of the outer wall of the base (101), a telescopic plate (702) is installed in each of the installation grooves (1011), and the telescopic end of each of the telescopic plates (702) is located outside the installation groove (1011), and three side plates (701) are evenly fixed to the outer walls of the two transparent covers (7), the bottom end of each of the side plates (701) is screwed to the upper surface of the telescopic end of the telescopic plate (702), and a ball bearing is embedded on the lower surface of the telescopic end of each of the telescopic plates (702).

Images