Novel planting system construction method based on crop rotation of transgenic crops and common crops

Abstract

The invention relates to the field of agricultural planting, and particularly discloses a novel planting system construction method based on crop rotation of transgenic crops and common crops. Comprising the following steps: step 1, setting a test area: setting the test area at least comprising the following six planting treatments: transgenic soybean continuous cropping, common soybean continuous cropping, common corn continuous cropping, transgenic corn continuous cropping, common corn-transgenic soybean-transgenic soybean crop rotation, and transgenic corn-transgenic soybean-transgenic soybean crop rotation; according to the method, the influence of the transgenic crops on the soil and the crops is comprehensively evaluated by setting various planting treatment test areas containing the transgenic crops and the common crops, so that a scientific basis is favorably provided for reasonable planting and utilization of the transgenic crops; by means of crop rotation, intercropping and the like, a single-type crop planting mode is broken, so that the directed evolution risk of weeds and diseases and insect pests is reduced, the use amount of pesticides is reduced, and the agricultural production cost is reduced.

Description

Technical Field

[0001] The invention belongs to the field of agricultural planting, and specifically relates to a method for constructing a new planting system based on crop rotation of genetically modified crops and common crops. Background Art

[0002] Genetically modified crops are plants that have been transformed through modern biotechnology by transferring certain foreign genes into another plant, allowing it to effectively express the corresponding product (peptide or protein) and exhibit corresponding traits (such as insect resistance, disease resistance, stress resistance, improved nutritional content, etc.). The improvement of these traits enables genetically modified crops to have higher yields, better quality, and stronger adaptability in agricultural production.

[0003] However, long-term cultivation of a single type of crop, whether genetically modified or traditional, can easily lead to the directed evolution of weeds and pests, making traditional pesticides and control measures gradually ineffective, increasing agricultural production costs, and causing damage to the ecological environment. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a method for constructing a new planting system based on crop rotation of genetically modified crops and conventional crops, so as to solve the problem that the prior art involves long-term planting of a single type of crop, whether it is a genetically modified crop or a conventional crop, which easily leads to the directed evolution of weeds and pests, making traditional pesticides and control measures gradually ineffective, increasing agricultural production costs, and also causing damage to the ecological environment.

[0005] A new method for constructing a cropping system based on the rotation of genetically modified crops and conventional crops includes:

[0006] Step 1. Set up the test area: Set up a test area that includes at least the following six planting treatments: continuous cropping of genetically modified soybeans, continuous cropping of common soybeans, continuous cropping of common corn, continuous cropping of genetically modified corn, rotation of common corn-genetically modified soybeans-genetically modified soybeans, and rotation of genetically modified corn-genetically modified soybeans-genetically modified soybeans;

[0007] Step 2: Soil sample collection and monitoring:

[0008] Before sowing, at key growth points of crops, and after harvesting crops, soil samples of 0-20 cm, 20-40 cm, and 40-60 cm layers were collected in each of the above-mentioned treatment areas using a five-point sampling method; the physical and chemical properties of the soil samples were monitored, and the soil quality was quantitatively evaluated using a comprehensive evaluation model for soil physical and chemical properties;

[0009] Step 3: Microbial community diversity research: Use high-throughput sequencing methods to conduct in-depth analysis of the microbial community diversity of the collected soil samples, including the types, quantity, distribution, structure and functional characteristics of microorganisms, and use the microbial community diversity index calculation formula to quantitatively evaluate the diversity of microbial communities;

[0010] Step 4: Crop growth and yield monitoring: monitor and record crop growth and yield indicators in each treatment area at the same time, and use the following crop growth and yield comprehensive evaluation model to quantitatively evaluate crop growth and yield;

[0011] Step 5: Data analysis and system construction:

[0012] Based on the collected soil sample data and crop growth and yield data, statistical analysis was conducted to evaluate the effects of different planting treatments on soil quality and crop yield. Combined with the research results of microbial community diversity, a new planting system was constructed that can not only effectively solve the problem of wild soybean weeds caused by continuous soybean cropping, but also simulate the problems that may be caused by the large-scale promotion of genetically modified crops in the future, and solve the problem of directed evolution of weeds and pests caused by the planting of a single type of crop;

[0013] Step 6: Technology implantation and consideration: In the process of constructing the new cropping system, it is also necessary to consider the implantation of common corn and soybean herbicide general technology in the rotation of common corn-GM soybean-GM soybean, so as to further solve the problem of wild soybean weeds caused by continuous soybean cropping;

[0014] Step 7: Field trial verification: After the new planting system is constructed, long-term field trial verification is carried out to ensure its feasibility and stability in actual agricultural production.

[0015] Preferably, the key nodes include but are not limited to the seedling stage, flowering stage, and maturity stage.

[0016] Preferably, the physical and chemical properties include but are not limited to soil pH, organic matter content, and nutrient content.

[0017] Preferably, in step 2, the comprehensive evaluation model of soil physical and chemical properties is as follows:

[0018]

[0019] Among them, P soil is the comprehensive evaluation value of soil physical and chemical properties, z represents soil depth, pH(z), ORG(z), and NUT(z) represent the pH value, organic matter content, and nutrient content of the soil at depth z, respectively. pH (z),ω org (z),ω nut (z) is the weight function corresponding to the physicochemical properties.

[0020] Preferably, in step 3, the microbial community diversity index calculation formula is as follows:

[0021]

[0022] Among them, D micro is the diversity index of microbial communities, N is the total number of microbial species, and p i is the relative abundance of the i-th microorganism, ω func (i) is the weight of the functional characteristics of the i-th microorganism.

[0023] Preferably, in step 4, the crop growth and yield comprehensive evaluation model is as follows:

[0024]

[0025] Among them, G crop is the comprehensive evaluation value of crop growth and yield, M is the total number of crop growth indicators and yield indicators, g j (t) is the value of the j-th crop growth index at time t, y j (t) is the value of the j-th crop yield index at time t, ω grow (j,t),ω yield (j, t) are the weights of the corresponding growth index and yield index respectively.

[0026] Preferably, in step 4, the crop growth indicators include plant height, leaf area, and biomass;

[0027] The yield indicators include yield per unit area and thousand-grain weight.

[0028] Preferably, step seven further comprises:

[0029] Continuously monitor changes in microbial community diversity and quantify them using the above-mentioned microbial community diversity index to verify the long-term impact on soil health and crop growth;

[0030] Soil quality and crop growth are continuously monitored and evaluated using a comprehensive assessment model of soil physical and chemical properties and a comprehensive assessment model of crop growth and yield.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] Through crop rotation and intercropping, the pattern of planting a single type of crop is broken, thereby reducing the risk of directed evolution of weeds and pests, helping to reduce the use of pesticides, reduce agricultural production costs, and also help protect the ecological environment;

[0033] By setting up test areas with multiple planting treatments that include genetically modified crops and conventional crops, the impacts of genetically modified crops on soil and crops were comprehensively evaluated, which helps provide a scientific basis for the rational planting and utilization of genetically modified crops. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the method of the present invention. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] like Figure 1 As shown:

[0037] Embodiment 1: The present invention provides a method for constructing a new planting system based on crop rotation of transgenic crops and common crops, comprising:

[0038] Step 1. Set up the test area: Set up a test area that includes at least the following six planting treatments: continuous cropping of genetically modified soybeans, continuous cropping of common soybeans, continuous cropping of common corn, continuous cropping of genetically modified corn, rotation of common corn-genetically modified soybeans-genetically modified soybeans, and rotation of genetically modified corn-genetically modified soybeans-genetically modified soybeans;

[0039] Step 2: Soil sample collection and monitoring:

[0040] Before sowing, at key growth points of crops, and after harvesting crops, soil samples of 0-20 cm, 20-40 cm, and 40-60 cm layers were collected in each of the above-mentioned treatment areas using a five-point sampling method; the physical and chemical properties of the soil samples were monitored, and the soil quality was quantitatively evaluated using a comprehensive evaluation model for soil physical and chemical properties;

[0041] Step 3: Microbial community diversity research: Use high-throughput sequencing methods to conduct in-depth analysis of the microbial community diversity of the collected soil samples, including the types, quantity, distribution, structure and functional characteristics of microorganisms, and use the microbial community diversity index calculation formula to quantitatively evaluate the diversity of microbial communities;

[0042] Step 4: Crop growth and yield monitoring: monitor and record crop growth and yield indicators in each treatment area at the same time, and use the following crop growth and yield comprehensive evaluation model to quantitatively evaluate crop growth and yield;

[0043] Step 5: Data analysis and system construction:

[0044] Based on the collected soil sample data and crop growth and yield data, statistical analysis was conducted to evaluate the effects of different planting treatments on soil quality and crop yield. Combined with the research results of microbial community diversity, a new planting system was constructed that can not only effectively solve the problem of wild soybean weeds caused by continuous soybean cropping, but also simulate the problems that may be caused by the large-scale promotion of genetically modified crops in the future, and solve the problem of directed evolution of weeds and pests caused by the planting of a single type of crop;

[0045] Step 6: Technology implantation and consideration: In the process of constructing a new cropping system, it is also necessary to consider the implantation of common corn and soybean herbicide general technology in the rotation of common corn-GM soybean-GM soybean to further solve the problem of wild soybean weeds caused by continuous soybean cropping;

[0046] Step 7: Field trial verification: After the new planting system is constructed, long-term field trial verification is carried out to ensure its feasibility and stability in actual agricultural production.

[0047] As can be seen from the above, the present invention proposes a new planting system construction method based on the rotation of transgenic crops and ordinary crops. The method aims to solve the problems of weed management, soil quality decline and directed evolution of crop diseases and insect pests faced in current agricultural production through systematic experimental design and scientific data analysis. Specifically, the method first sets up a test area containing multiple planting treatments such as transgenic soybeans, ordinary soybeans, transgenic corn and ordinary corn to comprehensively evaluate the effects of different planting patterns on soil and crops;

[0048] During the experiment, soil samples were collected at different soil depths to monitor their physical and chemical properties, and a comprehensive assessment model was used to quantitatively evaluate soil quality. At the same time, high-throughput sequencing technology was used to conduct an in-depth analysis of soil microbial community diversity to reveal the effects of different planting treatments on microbial community structure and function. In addition, crop growth and yield indicators were monitored to evaluate crop production performance under different planting patterns.

[0049] Based on the above data, the present invention evaluates the effects of different planting treatments on soil quality and crop yield through statistical analysis methods, and constructs a new planting system in combination with the research results of microbial community diversity; this system can not only effectively solve the problem of wild soybean weeds caused by continuous soybean cropping, but also simulate the challenges that may be brought about by the large-scale promotion of genetically modified crops in the future, and solve the problem of directed evolution of weeds and pests caused by the planting of a single type of crop;

[0050] In the process of constructing a new cropping system, the present invention also considers the implantation of general herbicide technology in ordinary corn-transgenic soybean rotation to further improve the effect of weed management. Finally, through long-term field test verification, the feasibility and stability of the new cropping system in actual agricultural production are ensured, providing useful reference and guidance for agricultural production.

[0051] Embodiment 2: This embodiment is basically the same as the previous embodiment, except that key nodes include but are not limited to seedling stage, flowering stage, and maturity stage.

[0052] Specifically, the physical and chemical properties include, but are not limited to, soil pH, organic matter content, and nutrient content.

[0053] Specifically, in step 2, the comprehensive evaluation model of soil physical and chemical properties is as follows:

[0054]

[0055] Among them, P soil is the comprehensive evaluation value of soil physical and chemical properties, z represents soil depth, pH(z), ORG(z), and NUT(z) represent the pH value, organic matter content, and nutrient content of the soil at depth z, respectively. pH (z),ω org (z),ω nut (z) is the weight function corresponding to the physicochemical properties.

[0056] Specifically, in step three, the calculation formula of the microbial community diversity index is as follows:

[0057]

[0058] Among them, D micro is the diversity index of microbial communities, N is the total number of microbial species, and p i is the relative abundance of the i-th microorganism, ω func (i) is the weight of the functional characteristics of the i-th microorganism.

[0059] Specifically, in step 4, the comprehensive evaluation model of crop growth and yield is as follows:

[0060]

[0061] Among them, G crop is the comprehensive evaluation value of crop growth and yield, M is the total number of crop growth indicators and yield indicators, g j (t) is the value of the j-th crop growth index at time t, y j (t) is the value of the j-th crop yield index at time t, ω grow (j,t),ω yield(j, t) are the weights of the corresponding growth index and yield index respectively.

[0062] As can be seen from the above, this embodiment emphasizes the importance of soil and crop monitoring at key nodes in the crop growth process (such as seedling stage, flowering stage, maturity stage, etc.), and lists in detail the specific contents of soil physical and chemical properties, including key indicators such as soil pH value, organic matter content, and nutrient content;

[0063] In the soil sample collection and monitoring steps, a comprehensive assessment model for soil physical and chemical properties was introduced. This model integrates the physical and chemical property data of different soil depths and assigns them corresponding weight functions, so as to more comprehensively assess soil quality. This quantitative assessment method helps to accurately reflect the changes in soil under different planting treatments and provide a scientific basis for the subsequent construction of planting systems.

[0064] At the same time, this embodiment also describes in detail the calculation method of the microbial community diversity index, which uses high-throughput sequencing technology to obtain soil microbial community information, and calculates parameters such as the total number of microbial species, relative abundance, and functional characteristic weights to obtain the microbial community diversity index. This index can objectively reflect the diversity and complexity of soil microbial communities, and provides a powerful tool for evaluating the impact of different planting treatments on soil health;

[0065] In addition, this embodiment also proposes a comprehensive evaluation model for crop growth and yield, which comprehensively considers the growth indicators and yield indicators of crops and assigns them corresponding weights, so as to more comprehensively evaluate the growth performance and yield level of crops. This evaluation method helps to accurately reflect the impact of different planting treatments on crop growth and yield, and provides an important reference for optimizing the planting system.

[0066] Embodiment 3: This embodiment is basically the same as the previous embodiment, except that in step 4, the crop growth indicators include plant height, leaf area, and biomass;

[0067] Yield indicators include yield per unit area and thousand-grain weight.

[0068] Specifically, step seven also includes:

[0069] Continuously monitor changes in microbial community diversity and quantify them using the above-mentioned microbial community diversity index to verify the long-term impact on soil health and crop growth;

[0070] Soil quality and crop growth are continuously monitored and evaluated using a comprehensive assessment model of soil physical and chemical properties and a comprehensive assessment model of crop growth and yield.

[0071] As can be seen from the above, based on the previous embodiments, this embodiment further refines the specific contents of the crop growth index and the yield index. In step 4, the crop growth index includes plant height, leaf area and biomass, which can directly reflect the growth status and growth rate of the crop; the yield index includes yield per unit area and thousand-grain weight, which are directly related to the economic value and yield stability of the crop;

[0072] In addition, this embodiment adds continuous monitoring and evaluation of microbial community diversity, soil quality and crop growth in step seven. By continuously monitoring the changes in microbial community diversity and using the microbial community diversity index for quantitative evaluation, we can gain an in-depth understanding of the long-term changing trends in soil health and the effects of different planting treatments on soil microbial communities. At the same time, by using the comprehensive evaluation model for soil physical and chemical properties and the comprehensive evaluation model for crop growth and yield to continuously monitor and evaluate soil quality and crop growth, we can promptly discover and resolve potential problems and ensure the stability and sustainability of the new planting system.

[0073] The standard parts used in the present invention can all be purchased from the market, and the special-shaped parts can be customized according to the description and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0074] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0075] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0076] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0077] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0078] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0079] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for constructing a new planting system based on crop rotation of genetically modified crops and ordinary crops, characterized in that: include: Step 1. Set up the test area: Set up a test area that includes at least the following six planting treatments: continuous cropping of genetically modified soybeans, continuous cropping of common soybeans, continuous cropping of common corn, continuous cropping of genetically modified corn, rotation of common corn-genetically modified soybeans-genetically modified soybeans, and rotation of genetically modified corn-genetically modified soybeans-genetically modified soybeans; Step 2: Soil sample collection and monitoring: Before sowing, at key growth points of crops, and after harvesting crops, soil samples of 0-20 cm, 20-40 cm, and 40-60 cm layers were collected in each of the above-mentioned treatment areas using a five-point sampling method; the physical and chemical properties of the soil samples were monitored, and the soil quality was quantitatively evaluated using a comprehensive evaluation model for soil physical and chemical properties; Step 3: Microbial community diversity research: Use high-throughput sequencing methods to conduct in-depth analysis of the microbial community diversity of the collected soil samples, including the types, quantity, distribution, structure and functional characteristics of microorganisms, and use the microbial community diversity index calculation formula to quantitatively evaluate the diversity of microbial communities; Step 4: Crop growth and yield monitoring: monitor and record crop growth and yield indicators in each treatment area at the same time, and use the following crop growth and yield comprehensive evaluation model to quantitatively evaluate crop growth and yield; Step 5: Data analysis and system construction: Based on the collected soil sample data and crop growth and yield data, statistical analysis was conducted to evaluate the effects of different planting treatments on soil quality and crop yield. Combined with the research results of microbial community diversity, a new planting system was constructed that can effectively solve the problem of wild soybean weeds caused by continuous soybean cropping, simulate the problems that may be caused by the large-scale promotion of genetically modified crops in the future, and solve the problem of directed evolution of weeds and pests caused by the planting of a single type of crop; Step 6: Technology implantation and consideration: In the process of constructing the new cropping system, it is also necessary to consider the implantation of common corn and soybean herbicide general technology in the rotation of common corn-GM soybean-GM soybean, so as to further solve the problem of wild soybean weeds caused by continuous soybean cropping; Step 7: Field trial verification: After the new planting system is constructed, long-term field trial verification is carried out to ensure its feasibility and stability in actual agricultural production.

2. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 1, characterized in that: The key nodes include but are not limited to the seedling stage, flowering stage, and maturity stage.

3. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 2, characterized in that: The physical and chemical properties include, but are not limited to, soil pH, organic matter content, and nutrient content.

4. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 3, characterized in that: In step 2, the comprehensive evaluation model of soil physical and chemical properties is as follows: Among them, P soil is the comprehensive evaluation value of soil physical and chemical properties, z represents soil depth, pH(z), ORG(z), and NUT(z) represent the pH value, organic matter content, and nutrient content of the soil at depth z, respectively. pH (z),ω org (z),ω nut (z) is the weight function corresponding to the physicochemical properties.

5. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 4, characterized in that: In step 3, the microbial community diversity index is calculated as follows: Among them, D micro is the diversity index of microbial communities, N is the total number of microbial species, and p i is the relative abundance of the i-th microorganism, ω func (i) is the weight of the functional characteristics of the i-th microorganism.

6. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 5, characterized in that: In step 4, the comprehensive evaluation model of crop growth and yield is as follows: Among them, G crop is the comprehensive evaluation value of crop growth and yield, M is the total number of crop growth indicators and yield indicators, g j (t) is the value of the j-th crop growth index at time t, y j (t) is the value of the j-th crop yield index at time t, ω grow (j,t),ω yield (j, t) are the weights of the corresponding growth index and yield index respectively.

7. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 1, characterized in that: In step 4, the crop growth indicators include plant height, leaf area, and biomass; The yield indicators include yield per unit area and thousand-grain weight.

8. The method for constructing a new planting system based on crop rotation of genetically modified crops and common crops as claimed in claim 1, characterized in that: Step seven also includes: Continuously monitor changes in microbial community diversity and quantify them using the above-mentioned microbial community diversity index to verify the long-term impact on soil health and crop growth; Soil quality and crop growth are continuously monitored and evaluated using a comprehensive assessment model of soil physical and chemical properties and a comprehensive assessment model of crop growth and yield.

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