Cordyceps militaris liquid strain large-scale culture method, cordyceps militaris cultured by cordyceps militaris liquid strain large-scale culture method and application of cordyceps militaris

Abstract

The invention discloses a cordyceps militaris liquid strain large-scale culture method, cordyceps militaris cultured by the cordyceps militaris liquid strain large-scale culture method and application of the cordyceps militaris liquid strain large-scale culture method, and belongs to the technical field of cordyceps militaris. The large-scale culture method of the cordyceps militaris liquid strain comprises the following steps: adding a liquid culture medium into a liquid strain fermentation tank, inoculating the cordyceps militaris strain according to 3-5% of the weight of the culture medium, and keeping the tank pressure at 0.05-0.07 MP; filtering air is continuously introduced, and the ventilation volume is 0.3-0.5 m < 3 > / h; the fermentation time is 72 to 84 hours; the cordyceps militaris strain is a cordyceps militaris strain CICC14014, a cordyceps militaris strain CICC14015 and / or a cordyceps militaris strain CICC14013. According to the method, the cordyceps militaris strain with vigorous hyphae, moderate sporocarp density, high yield and high cordycepin content is obtained by limiting the culture process, namely the composition of the culture medium, the strain inoculation amount, the ventilation amount and the fermentation time.

Description

Technical Field

[0001] The present application relates to a method for large-scale cultivation of liquid strains of Cordyceps militaris, the cultivated Cordyceps militaris and applications thereof, belonging to the technical field of Cordyceps militaris. Background Art

[0002] Cordyceps militaris, also known as northern cordyceps and northern winter cordyceps, is an insect-fungus complex formed by fungi parasitizing on the bodies of insects such as Lepidoptera. It is a complex composed of two parts: the fruiting body (i.e. the grass part) and the sclerotium (i.e. the body part of the insect). In winter, the larvae hibernate in the soil, and the fungi parasitize in them, absorbing nutrients, and the larvae die of mycelium. In summer, seedlings grow from the bodies of the larvae, which are grass-like and collected around the summer solstice. It is a nutritious product with a tonic effect. Cordyceps militaris contains a variety of biologically active substances, including cordycepin, adenosine and other nucleoside substances, as well as cordyceps polysaccharides, superoxide dismutase and amino acids. The most important active ingredient is cordycepin. Studies have shown that cordycepin has the effects of regulating immunity, anti-tumor, anti-inflammatory, improving metabolism, and scavenging free radicals. At this stage, cordycepin is becoming an extremely active field in pharmaceutical chemistry, anti-aging, beauty, and health products. Cordyceps militaris is distributed worldwide. The number of natural resources is very small, and the market demand is very large.

[0003] Therefore, achieving large-scale cultivation of Cordyceps militaris will produce huge economic and social benefits. Under what process conditions can Cordyceps militaris strains with vigorous mycelium, moderate fruiting body density, high yield and high cordycepin content be cultivated is an important issue that urgently needs to be solved in the field of Cordyceps militaris technology today. Summary of the invention

[0004] In order to solve the above problems, a method for large-scale cultivation of liquid strains of Cordyceps militaris, Cordyceps militaris cultivated therefrom and applications thereof are provided, wherein the Cordyceps militaris strains with vigorous mycelium, moderate fruiting body density, high yield and high cordycepin content are obtained by limiting the cultivation process, i.e., the composition of the culture medium, the amount of strain access, the ventilation volume and the fermentation time.

[0005] According to one aspect of the present application, a method for large-scale cultivation of liquid Cordyceps militaris spawn is provided, comprising the following steps:

[0006] (1) adding liquid culture medium into a liquid strain fermentation tank, inoculating Cordyceps militaris strains at a weight ratio of 3% to 5% of the culture medium, and the tank pressure is 0.05 to 0.07 MPa;

[0007] (2) Filtered air is continuously introduced with a ventilation volume of 0.3 to 0.5 m 3 / h; fermentation time is 72~84h;

[0008] The Cordyceps militaris strains are Cordyceps militaris strains CICC14014, CICC14015 and / or CICC14013.

[0009] Optionally, in step (1), Cordyceps militaris spawn is inoculated into the culture medium at a weight ratio of 3%.

[0010] Optionally, in step (2), the ventilation volume is 0.5m 3 / h.

[0011] Optionally, in step (2), the fermentation time is 78 hours.

[0012] Optionally, the liquid culture medium comprises the following components: potatoes, glucose, peptone, glycine, KH2PO4, MgSO4, vitamin B1, water and fullerene suspension; wherein, by weight, the components are 8 to 10 parts of potatoes, 0.8 to 1 part of glucose, 0.3 to 0.5 part of peptone, 0.05 to 0.1 part of glycine, 0.1 to 0.15 part of KH2PO4, 0.05 to 0.07 part of MgSO4, 0.0005 to 0.001 part of vitamin B1, 45 to 55 parts of water and 0.0025 to 0.003 part of fullerene suspension.

[0013] Optionally, the method for preparing the fullerene suspension comprises the following steps:

[0014] (1) Weighing 8-10 g of starch, pyrolyzing the starch under normal pressure, heating at a rate of 10-15° C. / min, maintaining the temperature at 750-850° C. for 25-35 min, and then cooling to room temperature to obtain a pyrolysis carbon black product;

[0015] (2) mixing the pyrolysis carbon black product with concentrated sulfuric acid, reacting at room temperature for 24 to 30 hours, quenching after the reaction, washing with distilled water until neutral, and drying to obtain activated carbon black;

[0016] (3) placing activated carbon black and sodium borohydride in a quartz tube, sealing the tube, heating the tube at 850-950° C. for 3-3.5 h, and cooling the tube to obtain a fullerene product; washing the tube with acetone until no color reaction is observed on phenol red test paper, and drying the tube for later use;

[0017] (4) Mixing the dried fullerene product with polyethylene glycol, heating to 50-70° C., reacting for 0.5-1 h, and cooling to room temperature to obtain a fullerene suspension.

[0018] Optionally, in step (2), the mass ratio of the carbon black product to concentrated sulfuric acid is 1:35-37.

[0019] Optionally, in step (3), the mass ratio of activated carbon black to sodium borohydride is 1:10-12; and in step (4), the mass ratio of fullerene product to polyethylene glycol is 1:10-12.

[0020] According to another aspect of the present application, the present application also provides Cordyceps militaris cultured by the above-mentioned culture method.

[0021] According to another aspect of the present application, the present application also provides the use of the above-mentioned Cordyceps militaris in preparing products comprising Cordyceps militaris.

[0022] The beneficial effects of this application include but are not limited to:

[0023] 1. According to a method for large-scale cultivation of liquid Cordyceps militaris strains of the present application, by limiting the cultivation process, that is, the composition of the culture medium, the amount of strain access, the ventilation volume and the fermentation time, a Cordyceps militaris strain with vigorous mycelium, moderate fruiting body density, high yield and high cordycepin content is obtained.

[0024] 2. According to the liquid culture medium for large-scale cultivation of liquid strains of Cordyceps militaris of the present application, a culture medium suitable for the growth of Cordyceps militaris strains CICC14014, CICC14015 and / or CICC14013 is obtained through a specific ratio of specific components, so that the mycelium of Cordyceps militaris grows better and the cordycepin content is higher.

[0025] 3. According to the liquid culture medium for large-scale cultivation of liquid strains of Cordyceps militaris of the present application, a specific proportion of fullerene suspension is added to the liquid culture medium. On the one hand, fullerene has a large specific surface area and has micropores and defective structures, which can adsorb various nutrients and provide a richer and more stable biochemical environment for Cordyceps militaris; on the other hand, the fullerene surface has many aromatic rings and conjugated double bond structures, and Cordyceps militaris cells can be adsorbed on its surface through π-π stacking to form a three-dimensional network structure, providing a place for Cordyceps militaris to attach and grow.

[0026] 4. According to the large-scale cultivation method of Cordyceps militaris liquid spawn provided in this application, the process steps and equipment are simple, easy to operate, and have low production costs, and are suitable for use in general spawn factories. DETAILED DESCRIPTION

[0027] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0028] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. The reagents or raw materials used in the present invention can be purchased through conventional channels. Unless otherwise specified, the reagents or raw materials used in the present invention are used in a conventional manner in the art or in accordance with the product instructions. In addition, any method and material similar or equivalent to the described content can be applied to the method of the present invention. The preferred implementation methods and materials described in this patent are for demonstration purposes only.

[0029] The Cordyceps militaris strains used in this application have a deposit number of CICC14014, CICC14015 and / or CICC14013. The Latin name of Cordyceps militaris is Cordyceps militaris, and the strains CICC14014, CICC 14015 and / or CICC14013 are all purchased from the China Industrial Microbiological Culture Collection Administration Center.

[0030] Example 1

[0031] (1) adding liquid culture medium into a liquid strain fermentation tank, inoculating Cordyceps militaris strains at a weight ratio of 5% of the culture medium, and the tank pressure is 0.07 MPa;

[0032] The liquid culture medium includes: 10 parts of potato, 1 part of glucose, 0.5 parts of peptone, 0.1 parts of glycine, 0.15 parts of KH2PO4, 0.07 parts of MgSO4, 0.001 parts of vitamin B1, 55 parts of water and 0.003 parts of fullerene suspension;

[0033] (2) Filtered air is continuously introduced with a ventilation volume of 0.5m 3 / h; fermentation time is 84h;

[0034] The Cordyceps militaris strain is the Cordyceps militaris strain CICC14014.

[0035] The method for preparing the fullerene suspension comprises the following steps:

[0036] (1) Weighing 10 g of starch, pyrolyzing the starch under normal pressure, heating at a rate of 15° C. / min, maintaining the temperature at 850° C. for 35 min, and then cooling to room temperature to obtain a pyrolysis carbon black product;

[0037] (2) Mixing the pyrolysis carbon black product with concentrated sulfuric acid, reacting at room temperature for 30 hours, quenching after the reaction, washing with distilled water until neutral, and drying to obtain activated carbon black; the mass ratio of the carbon black product to the concentrated sulfuric acid is 1:37;

[0038] (3) placing activated carbon black and sodium borohydride in a quartz tube, sealing it, heating it at 950°C for 3.5 hours, and cooling it to obtain a fullerene product; washing it with acetone until no color reaction is observed on phenol red test paper, and drying it for later use; the mass ratio of activated carbon black to sodium borohydride is 1:12;

[0039] (4) The dried fullerene product is mixed with polyethylene glycol in a mass ratio of 1:12, heated to 70° C., reacted for 1 h, and cooled to room temperature to obtain a fullerene suspension.

[0040] Example 2

[0041] (1) adding liquid culture medium into a liquid strain fermentation tank, inoculating Cordyceps militaris strains at a weight ratio of 3% of the culture medium, and the tank pressure is 0.05 MPa;

[0042] The liquid culture medium includes: 8 parts of potato, 0.8 parts of glucose, 0.3 parts of peptone, 0.05 parts of glycine, 0.1 parts of KH2PO4, 0.05 parts of MgSO4, 0.0005 parts of vitamin B1, 45 parts of water and 0.0025 parts of fullerene suspension;

[0043] (2) Filtered air is continuously introduced with a ventilation volume of 0.3m 3 / h; fermentation time is 72h;

[0044] The Cordyceps militaris strain is the Cordyceps militaris strain CICC14013.

[0045] The method for preparing the fullerene suspension comprises the following steps:

[0046] (1) Weighing 8 g of starch, pyrolyzing the starch under normal pressure, heating at a rate of 10° C. / min, maintaining the temperature at 750° C. for 25 min, and then cooling to room temperature to obtain a pyrolysis carbon black product;

[0047] (2) Mixing the pyrolysis carbon black product with concentrated sulfuric acid, reacting at room temperature for 24 hours, quenching after the reaction, washing with distilled water until neutral, and drying to obtain activated carbon black; the mass ratio of the carbon black product to the concentrated sulfuric acid is 1:35;

[0048] (3) placing activated carbon black and sodium borohydride in a quartz tube, sealing it, heating it at 850°C for 3 hours, and cooling it to obtain a fullerene product; washing it with acetone until there is no color reaction on the phenol red test paper, and drying it for later use; the mass ratio of activated carbon black to sodium borohydride is 1:10;

[0049] (4) The dried fullerene product is mixed with polyethylene glycol in a mass ratio of 1:10, heated to 50° C., reacted for 0.5 h, and cooled to room temperature to obtain a fullerene suspension.

[0050] Example 3

[0051] (1) adding liquid culture medium into a liquid strain fermentation tank, inoculating Cordyceps militaris strains at a weight ratio of 4% of the culture medium, and the tank pressure is 0.06 MPa;

[0052] The liquid culture medium includes: 9 parts of potato, 0.9 parts of glucose, 0.4 parts of peptone, 0.07 parts of glycine, 0.12 parts of KH2PO4, 0.06 parts of MgSO4, 0.0007 parts of vitamin B1, 50 parts of water and 0.0027 parts of fullerene suspension;

[0053] (2) Filtered air is continuously introduced with a ventilation volume of 0.4m 3 / h; fermentation time is 78h;

[0054] The Cordyceps militaris strain is the Cordyceps militaris strain CICC14015.

[0055] The method for preparing the fullerene suspension comprises the following steps:

[0056] (1) Weighing 9 g of starch, pyrolyzing the starch under normal pressure, heating at a rate of 12° C. / min, maintaining the temperature at 800° C. for 30 min, and then cooling to room temperature to obtain a pyrolysis carbon black product;

[0057] (2) Mixing the pyrolysis carbon black product with concentrated sulfuric acid, reacting at room temperature for 26 hours, quenching after the reaction, washing with distilled water until neutral, and drying to obtain activated carbon black; the mass ratio of the carbon black product to the concentrated sulfuric acid is 1:36;

[0058] (3) placing activated carbon black and sodium borohydride in a quartz tube, sealing it, heating it at 900°C for 3h, and cooling it to obtain a fullerene product; washing it with acetone until no color reaction is observed on phenol red test paper, and drying it for later use; the mass ratio of activated carbon black to sodium borohydride is 1:11;

[0059] (4) The dried fullerene product is mixed with polyethylene glycol in a mass ratio of 1:11, heated to 60° C., reacted for 0.7 h, and cooled to room temperature to obtain a fullerene suspension.

[0060] Example 4

[0061] (1) adding liquid culture medium into a liquid strain fermentation tank, inoculating Cordyceps militaris strains at a weight ratio of 3% of the culture medium, and the tank pressure is 0.05 MPa;

[0062] The liquid culture medium includes: 9 parts of potato, 0.9 parts of glucose, 0.4 parts of peptone, 0.07 parts of glycine, 0.12 parts of KH2PO4, 0.06 parts of MgSO4, 0.0007 parts of vitamin B1, 50 parts of water and 0.0027 parts of fullerene suspension;

[0063] (2) Filtered air is continuously introduced with a ventilation volume of 0.5m 3 / h; fermentation time is 78h;

[0064] The Cordyceps militaris strain is the Cordyceps militaris strain CICC14015.

[0065] The method for preparing the fullerene suspension comprises the following steps:

[0066] (1) Weighing 10 g of starch, pyrolyzing the starch under normal pressure, heating at a rate of 10° C. / min, maintaining the temperature at 800° C. for 30 min, and then cooling to room temperature to obtain a pyrolysis carbon black product;

[0067] (2) Mixing the pyrolysis carbon black product with concentrated sulfuric acid, reacting at room temperature for 24 hours, quenching after the reaction, washing with distilled water until neutral, and drying to obtain activated carbon black; the mass ratio of the carbon black product to the concentrated sulfuric acid is 1:36;

[0068] (3) placing activated carbon black and sodium borohydride in a quartz tube, sealing it, heating it at 900°C for 3h, and cooling it to obtain a fullerene product; washing it with acetone until no color reaction is observed on phenol red test paper, and drying it for later use; the mass ratio of activated carbon black to sodium borohydride is 1:10;

[0069] (4) The dried fullerene product is mixed with polyethylene glycol in a mass ratio of 1:10, heated to 60° C., reacted for 0.5 h, and cooled to room temperature to obtain a fullerene suspension.

[0070] Comparative Example 1

[0071] The difference between Comparative Example 1 and Example 4 is that the ventilation volume is 0.3m 3 / h, and the rest are the same.

[0072] Comparative Example 2

[0073] The difference between Comparative Example 2 and Example 4 is that the ventilation volume is 0.8m 3 / h, and the rest are the same.

[0074] Comparative Example 3

[0075] The difference between Comparative Example 3 and Example 4 is that the inoculation amount is 1%, and the rest are the same.

[0076] Comparative Example 4

[0077] The difference between Comparative Example 4 and Example 4 is that the inoculation amount is 5%, and the rest are the same.

[0078] Comparative Example 5

[0079] The difference between Comparative Example 5 and Example 4 is that the inoculation amount is 8%, and the rest are the same.

[0080] Comparative Example 6

[0081] The difference between Comparative Example 6 and Example 4 is that the culture time is 60 h, and the rest are the same.

[0082] Comparative Example 7

[0083] The difference between Comparative Example 7 and Example 4 is that the culture time is 72 hours, and the rest are the same.

[0084] Comparative Example 8

[0085] The difference between Comparative Example 8 and Example 4 is that the culture time is 84 hours, and the rest are the same.

[0086] Comparative Example 9

[0087] The difference between Comparative Example 9 and Example 4 is that the culture time is 90 hours, and the rest are the same.

[0088] Comparative Example 10

[0089] The difference between Comparative Example 10 and Example 4 is that the liquid culture medium does not include the fullerene suspension, and the rest is the same.

[0090] Comparative Example 11

[0091] The difference between Comparative Example 11 and Example 4 is that in the preparation method of the fullerene suspension, the mass ratio of the fullerene product to the polyethylene glycol is 1:5, and the rest are the same.

[0092] Comparative Example 12

[0093] The difference between Comparative Example 12 and Example 4 is that in the preparation method of the fullerene suspension, the mass ratio of the fullerene product to the polyethylene glycol is 1:15, and the rest are the same.

[0094] Experimental Example 1 Effects of different ventilation rates on mycelial growth and cordycepin content of Cordyceps militaris strain CICC14015

[0095] The liquid strains obtained in Comparative Examples 1 to 2 and Example 4 were inoculated onto wheat grain cultivation medium, and the mycelium growth and cordycepin content were examined according to the provisions of the Ministry of Agriculture and Rural Affairs standard NY / T2116-2012. The results are shown in Table 1.

[0096] Table 1 Effects of different ventilation volumes

[0097]

[0098]

[0099] As shown in Table 1, with the increase of ventilation, the growth rate of the fungus increased, and the dry weight of mycelium and the content of cordycepin increased first and then decreased with the increase of ventilation. 3 / h condition, that is, Example 4 has the best mycelium growth state, and the cordycepin and mycelium dry weight are the highest. This may be because Cordyceps militaris is an aerobic bacteria, and the lower ventilation cannot meet the growth of functional Cordyceps militaris strains, resulting in anaerobicity and weakened mycelium growth. Excessive ventilation causes excessive dissolved oxygen, resulting in mycelium elongation, poor mycelium growth and state, and premature mycelium aging, which in turn affects the accumulation of mycelium dry matter and the content of active substances. Therefore, the optimal ventilation volume is 0.3-0.5m 3 / h.

[0100] Experimental Example 2 Effects of different inoculation amounts on mycelial growth and cordycepin content of Cordyceps militaris strain CICC14015

[0101] The liquid strains obtained in Comparative Examples 3 to 5 and Example 4 were inoculated onto wheat grain cultivation medium, and the mycelium growth and cordycepin content were examined according to the provisions of the Ministry of Agriculture and Rural Affairs standard NY / T2116-2012. The results are shown in Table 2.

[0102] Table 2 Effect of different inoculum amounts

[0103]

[0104]

[0105] As shown in Table 2, with the increase of inoculation amount, the growth rate of the strain increased. When the inoculation amount was 5%, the dry weight of the mycelium reached the maximum, but a few mycelium showed autolysis. The cordycepin content was the highest at 3% inoculation amount. Therefore, the optimal inoculation amount was 3% to 5%.

[0106] Experimental Example 3 Effects of different culture times on mycelial growth and cordycepin content of Cordyceps militaris strain CICC14015

[0107] The liquid strains obtained in Comparative Examples 6 to 9 and Example 4 were inoculated onto wheat grain cultivation medium, and the mycelium growth and cordycepin content were examined according to the provisions of the Ministry of Agriculture and Rural Affairs standard NY / T2116-2012. The results are shown in Table 3.

[0108] Table 3 Effects of different culture times

[0109]

[0110]

[0111] As shown in Table 3, with the extension of culture time, the amount of mycelium and the content of cordycepin gradually increased. When the mycelium state was cultivated for 72 hours, it reached the optimal state. At this time, the cordycepin content in the mycelium was the highest, but the dry weight of the mycelium was low. When the mycelium was cultivated for 78 hours, the dry weight of the mycelium was the largest, and the mycelium growth was optimal. The growth of the strain and the cordycepin content did not change much from 78 hours to 84 hours. After that, the growth of the strain entered a decline period, and the cordycepin content in the fruiting body decreased significantly. Because the optimal culture time is 78 to 84 hours.

[0112] Experimental Example 4 Effects of different liquid culture media on mycelial growth and cordycepin content of Cordyceps militaris strain CICC14015

[0113] The liquid strains obtained in Comparative Examples 10 to 12 and Examples 1 to 3 were inoculated onto wheat grain cultivation medium, and the mycelium growth and cordycepin content were examined according to the provisions of the Ministry of Agriculture and Rural Affairs standard NY / T2116-2012. The results are shown in Table 4.

[0114] Table 4 Effect of liquid culture medium

[0115]

[0116]

[0117] As can be seen from Table 4, the growth of mycelium, mycelium dry weight and cordycepin content in Comparative Example 10 are lower than those in Examples 1 to 4. The reason for this may be that no fullerene suspension was added to the liquid culture medium in Comparative Example 10, which has a certain impact on the large-scale cultivation of Cordyceps militaris. On the one hand, it is not conducive to the growth and attachment of Cordyceps militaris. On the other hand, it is not conducive to the adsorption of nutrients, providing Cordyceps militaris with a rich and stable biochemical environment, thereby affecting the growth of mycelium and the cordycepin content of Cordyceps militaris; Comparative Examples 11 and 12 changed the mass ratio of fullerene product to polyethylene glycol in the fullerene suspension, which also had an adverse effect on the growth of mycelium and the cordycepin content; in Comparative Example 11, the mass ratio of fullerene product to polyethylene glycol was 1:5, compared with Example 4, the proportion of fullerene products is increased, and the growth of mycelium and the content of cordycepin are both reduced. The reason for the analysis is that there are too many fullerene products, and the dispersibility is affected, resulting in fullerene agglomeration and even sedimentation, which in turn affects the growth of Cordyceps militaris; in Comparative Example 12, the mass ratio of fullerene products to polyethylene glycol is 1:15, compared with Example 4, the proportion of fullerene products is reduced, and the growth of mycelium and the content of cordycepin are both reduced. The reason for the analysis is that the fullerene products are reduced and the content is insufficient, which cannot meet the actual needs. Therefore, the mass ratio of fullerene products to polyethylene glycol in the present application is 1:(10-12), which can meet the actual needs on the one hand, and on the other hand, ensure its good dispersibility, which is conducive to the growth of Cordyceps militaris.

[0118] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A method for large-scale cultivation of liquid Cordyceps militaris spawn, characterized in that: The following steps are involved: (1) adding liquid culture medium into a liquid strain fermentation tank, inoculating Cordyceps militaris strains at a weight ratio of 3% to 5% of the culture medium, and the tank pressure is 0.05 to 0.07 MPa; (2) Filtered air is continuously introduced with a ventilation volume of 0.3 to 0.5 m 3 / h; fermentation time is 72~84h; The Cordyceps militaris strains are Cordyceps militaris strains CICC14014, CICC14015 and / or CICC14013.

2. The culture method according to claim 1, characterized in that In step (1), Cordyceps militaris spawn is inoculated into the culture medium at a weight ratio of 3%.

3. The culture method according to claim 1, characterized in that In step (2), the ventilation volume is 0.5m 3 / h.

4. The culture method according to claim 1, characterized in that In step (2), the fermentation time is 78h.

5. The culture method according to claim 1, characterized in that The liquid culture medium comprises the following components: potatoes, glucose, peptone, glycine, KH2PO4, MgSO4, vitamin B1, water and fullerene suspension; wherein, by weight, the components include 8 to 10 parts of potatoes, 0.8 to 1 part of glucose, 0.3 to 0.5 part of peptone, 0.05 to 0.1 part of glycine, 0.1 to 0.15 part of KH2PO4, 0.05 to 0.07 part of MgSO4, 0.0005 to 0.001 part of vitamin B1, 45 to 55 parts of water and 0.0025 to 0.003 part of fullerene suspension.

6. The culture method according to claim 5, characterized in that: The preparation method of the fullerene suspension comprises the following steps: (1) Weighing 8-10 g of starch, pyrolyzing the starch under normal pressure, heating at a rate of 10-15° C. / min, maintaining the temperature at 750-850° C. for 25-35 min, and then cooling to room temperature to obtain a pyrolysis carbon black product; (2) mixing the pyrolysis carbon black product with concentrated sulfuric acid, reacting at room temperature for 24 to 30 hours, quenching after the reaction, washing with distilled water until neutral, and drying to obtain activated carbon black; (3) placing activated carbon black and sodium borohydride in a quartz tube, sealing the tube, heating the tube at 850-950° C. for 3-3.5 h, and cooling the tube to obtain a fullerene product; washing the tube with acetone until no color reaction is observed on phenol red test paper, and drying the tube for later use; (4) Mixing the dried fullerene product with polyethylene glycol, heating to 50-70° C., reacting for 0.5-1 h, and cooling to room temperature to obtain a fullerene suspension.

7. The culture method according to claim 6, characterized in that: In step (2), the mass ratio of the carbon black product to the concentrated sulfuric acid is 1:35-37.

8. The culture method according to claim 6, characterized in that: In step (3), the mass ratio of activated carbon black to sodium borohydride is 1:10-12; and in step (4), the mass ratio of fullerene product to polyethylene glycol is 1:10-12.

9. Cordyceps militaris obtained by the cultivation method according to any one of claims 1 to 8.

10. Use of the Cordyceps militaris according to claim 9 in preparing products comprising Cordyceps militaris.

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