Subpackaging method of connected subpackaging bag and connected subpackaging bag

Abstract

The invention provides a subpackaging method of a connected-bag subpackaging bag and the connected-bag subpackaging bag, and relates to the technical field of biological agent subpackaging, the subpackaging method of the connected-bag subpackaging bag comprises the steps that cell sap is input into the connected-bag subpackaging bag through a second connecting part, so that each bag body is filled with the cell sap from bottom to top; when it is determined that the bag body located at the head part is full of the cell sap, stopping inputting the cell sap; and carrying out heat sealing on the head end and the tail end of each bag body through a heat sealing instrument, so that the cell sap with a preset volume is subpackaged in each bag body. The cell sap is stored by adopting the connected subpackaging bags which are designed in series, and the cell sap is input into the bag bodies from bottom to top, so that the emptying treatment of bubbles is realized, the precise preparation with the minimum unit capacity can be realized, artificial preparation in a sterile environment is not needed, the high requirement of the precise preparation is met, and the portable subpackaging effect is achieved; and the labor cost is effectively reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of biological preparation packaging, and in particular to a packaging method of a bag-in-a-bag packaging bag and a bag-in-a-bag packaging bag. Background Art

[0002] At present, the packaging of biological preparations is an important part of the field of biomedical technology and is crucial to cell therapy and pharmaceutical preparation. When packaging cell preparations, high precision is required because the processed cells are very scarce. In addition, when the minimum unit volume of the preparation is very small, the existing technology usually performs manual packaging in a sterile environment, which is easy to be contaminated, making the packaging of cell preparations more difficult. Summary of the invention

[0003] The invention provides a packaging method for connected bag packaging bags, which is used to solve the problems of low packaging accuracy and easy contamination in the packaging technology in the prior art.

[0004] The present invention provides a method for packaging a continuous bag-packing bag, comprising:

[0005] Inputting cell fluid into the inside of the linked bag dispensing bag through the second connecting part, so that the cell fluid fills each bag from bottom to top;

[0006] When it is determined that the bag at the head is full of cell fluid, the input of cell fluid is stopped;

[0007] The head end and the tail end of each bag body are heat-sealed by a heat-sealing instrument, so that a predetermined volume of cell fluid is filled in each bag body.

[0008] According to a bag-in-a-bag dispensing method provided by an embodiment of the present invention, the liquid outlet of the drip bucket is connected to the second connecting part through the first pipeline, and before the step of inputting cell fluid into the bag-in-a-bag dispensing bag through the second connecting part, the method further includes:

[0009] The drip bucket is driven to rotate so that the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet, thereby allowing the cell fluid in the drip bucket to be input into the interior of the bag-linking packaging bag through the second connecting part.

[0010] According to an embodiment of the present invention, a bag-to-bag dispensing method is provided, before the step of driving the drip bucket to rotate, further comprising:

[0011] The cell solution is input into the dropper for degassing.

[0012] According to a method for dispensing a connected bag-type dispensing bag provided by an embodiment of the present invention, a bubble sensor or a light sensor is provided at the first connecting portion; the step of stopping the input of the cell fluid when it is determined that the bag body at the head portion is full of the cell fluid comprises:

[0013] When the bubble sensor or the light sensor detects that the cell fluid flows through, the pump body is controlled to stop pumping the cell fluid into the linked bag dispensing bag.

[0014] According to a method for dispensing a bag-in-a-bag dispensing bag provided by an embodiment of the present invention, the first pipeline is connected in series with a pressure sensor or a flow sensor; the step of stopping the input of cell fluid when it is determined that the bag body at the head is full of cell fluid comprises:

[0015] When the pressure of the first pipeline is greater than or equal to a preset pressure value, or when the flow rate of the first pipeline is greater than or equal to a preset flow rate, the pump body is controlled to stop pumping cell fluid into the linked bag packaging bag.

[0016] According to an embodiment of the present invention, a method for dispensing a continuous bag dispensing bag is provided, wherein the continuous bag dispensing bag is connected to a weighing sensor, and the step of stopping the input of cell fluid when it is determined that the bag body at the head is full of cell fluid comprises:

[0017] Determine the weight of the cell fluid in the connected bag-packing bag according to the weight of the connected bag-packing bag and the total weight of the connected bag-packing bag after the cell fluid is input;

[0018] Determining the volume of the cell fluid in the bag-connecting dispensing bag according to the weight of the cell fluid in the bag-connecting dispensing bag;

[0019] When the cell fluid volume in the bag-linked dispensing bag is greater than or equal to a preset volume, the pump body is controlled to stop pumping the cell fluid into the bag-linked dispensing bag.

[0020] According to an embodiment of the present invention, a linked bag dispensing bag is provided, and the linked bag dispensing bag is based on a dispensing method of the linked bag dispensing bag described in any one of the above, comprising:

[0021] A plurality of bag bodies are connected end to end in sequence, and a connection passage for connecting the two adjacent bag bodies is formed at the connection point of the bag body located at the head, and a first connection part is provided at the bag body located at the tail, and a second connection part is provided.

[0022] According to a connected bag dispensing bag provided by an embodiment of the present invention, the capacity of each bag body is equal.

[0023] According to a connected bag dispensing bag provided by an embodiment of the present invention, the cross-section of the front end of each bag body gradually decreases along the first direction, and the cross-section of the rear end of the bag body gradually decreases along the second direction.

[0024] According to a bag-in-a-bag dispensing bag provided by an embodiment of the present invention, the first connecting part is used to communicate with a sterile filter, and the second connecting part is used to communicate with a liquid outlet of a drip bucket or a liquid outlet of a bubble removal container through a first pipeline.

[0025] The packaging method of the connected bag packaging bag provided in the embodiment of the present invention adopts a serially designed connected bag packaging bag to store cell fluid, and inputs the cell fluid into the bag body from bottom to top, thereby realizing the emptying of bubbles, and can achieve precise preparation of the minimum unit volume, without the need for manual preparation in a sterile environment, thus meeting the high requirements of precise preparation, having the effect of portable packaging, and effectively reducing labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 This is one of the flow diagrams of the method for packaging a continuous bag packaging bag provided by an embodiment of the present invention;

[0028] Figure 2 This is the second flow chart of the method for packaging the linked bag packaging bag provided by the embodiment of the present invention;

[0029] Figure 3 This is a third flow chart of a method for packaging a continuous bag-packing bag provided by an embodiment of the present invention;

[0030] Figure 4 This is a fourth flow chart of the method for packaging a continuous bag-packing bag provided by an embodiment of the present invention;

[0031] Figure 5 It is a schematic diagram of the side cross-sectional structure of the linked bag dispensing bag provided in an embodiment of the present invention.

[0032] Reference numerals: 100, bag-connecting dispensing bag; 110, bag body; 120, connecting channel; 130, head end; 140, tail end; 150, first connecting part; 160, second connecting part. DETAILED DESCRIPTION

[0033] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0034] In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships described in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limitations on the embodiments of the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0035] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0036] In the embodiments of the present invention, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0037] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0038] Figure 1 One of the flow charts of the method for packaging a continuous bag packing bag provided by an embodiment of the present invention is illustrated, such as Figure 1As shown, the packaging method of the continuous bag packaging bag comprises the following steps:

[0039] Step S100, injecting cell fluid into the inside of the linked bag-packaging bag 100 through the second connecting part 160, so that the cell fluid fills each bag body 110 from bottom to top;

[0040] The input of the cell fluid is completed by a pump body (not shown), which is connected in series to the first pipeline, that is, the pump body is connected in series between the liquid outlet of the drip bucket (not shown) and the second connection part 160. When the pump body is working, it squeezes the first pipeline, and the cell fluid is input into the inside of the bag-in-a-bag dispensing bag 100 under the action of pressure. In the process of continuously inputting the cell fluid into the bag-in-a-bag dispensing bag 100, the bag-in-a-bag dispensing bag 100 needs to be kept in a vertical state (the bag-in-a-bag dispensing bag 100 can be fixed by hanging vertically). At this time, the second connection part 160 is located at the bottom of the bag-in-a-bag dispensing bag 100, and the first connection part 150 is located at the top of the bag-in-a-bag dispensing bag 100. In the process of inputting the cell fluid, the cell fluid will fill each bag body 110 from bottom to top, and the bubbles will rise and be discharged through the first connection part 150 during the filling process, so that there are no bubbles inside the bag-in-a-bag dispensing bag 100 during the entire preparation process.

[0041] Furthermore, in order to ensure that the cell fluid has a better emptying effect, during the process of inputting the cell fluid into the bag-in-a-bag dispensing bag 100 , it is necessary to ensure that the flow rate of the input cell fluid is stable so that the rising cell fluid can discharge the air at any point in the bag-in-a-bag dispensing bag 100 .

[0042] It should be noted that the pump body in this embodiment is a peristaltic pump. Of course, the pump body may also be other types of pump bodies.

[0043] Step S200, when it is determined that the bag body 110 at the head is full of cell fluid, the input of cell fluid is stopped;

[0044] Step S300 , heat-seal the head end 130 and the tail end 140 of each bag body 110 by a heat-seal device, so that a predetermined volume of cell fluid is filled in each bag body 110 .

[0045] Since the capacity of each bag body 110 is fixed, after the head end 130 and the tail end 140 of each bag body 110 are heat-sealed, the cell fluid capacity in each bag body 110 is also fixed, thereby obtaining a cell preparation bag of the required capacity.

[0046] It should be noted here that the capacity of each bag body 110 can be designed according to specific needs, and the minimum capacity can be within 10 ml.

[0047] The packaging method of the connected bag packaging bag provided in the embodiment of the present invention adopts a serially designed connected bag packaging bag 100 to store cell fluid, and inputs the cell fluid into the bag body 110 from bottom to top, thereby realizing the emptying of bubbles, and can achieve precise preparation of the minimum unit volume, without the need for manual preparation in a sterile environment, thus meeting the high requirements of precise preparation, having the effect of portable packaging, and effectively reducing labor costs.

[0048] In one embodiment of the present invention, the first connection part 150 and the second connection part 160 of the bag-in-a-bag dispensing bag 100 are both connecting tubes, specifically, the first connection part 150 is a liquid outlet connecting tube, and the second connection part 160 is a liquid inlet connecting tube. Of course, the first connection part 150 and the second connection part 160 can also be in the form of connecting ports, specifically, the first connection part 150 is a connecting outlet of the bag-in-a-bag dispensing bag 100, and the second connection part 160 is a connecting inlet of the bag-in-a-bag dispensing bag 100. Providing the connecting part can facilitate the connection of the bag-in-a-bag dispensing bag 100 with the pipeline, specifically, the first connection part 150 is used to communicate with the sterile filter, and the second connection part 160 is used to communicate with the liquid outlet of the drip bucket or the liquid outlet of the bubble removal container through the first pipeline.

[0049] In an embodiment of the present invention, the liquid outlet of the drip bucket is connected to the second connection part 160 through the first pipeline. Before the step of inputting the cell solution into the bag-in-a-bag dispensing bag 100 through the second connection part 160, the dispensing method of the bag-in-a-bag dispensing bag further includes:

[0050] The drip bucket is driven to rotate so that the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet, thereby allowing the cell fluid in the drip bucket to be input into the bag-connecting dispensing bag 100 through the second connecting portion 160 .

[0051] The rotation of the drop bucket is driven by the drop bucket rotating assembly, the drop bucket is connected to the drop bucket rotating assembly, the drop bucket rotating assembly is electrically connected to the control unit, and the control unit controls the rotation of the drop bucket rotating assembly to drive the drop bucket to rotate. The control unit can be a control circuit board, a controller or a PLC, and the drop bucket rotating assembly is a common component in the field of biological preparation packaging technology, so the specific structure of the drop bucket rotating assembly is not introduced in detail here.

[0052] After driving the drip bucket to rotate, the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet. At this time, the cell fluid is continuously input into the drip bucket, and the cell fluid at the drip bucket liquid outlet pushes the cell fluid in the first pipeline to the inside of the bag-connecting packaging bag 100 through the second connecting portion 160 under the action of internal pressure. Since the bag-connecting packaging bag 100 is in a vertical state, after the cell fluid is pushed to the inside of the bag-connecting packaging bag 100 through the second connecting portion 160, the air inside the bag-connecting packaging bag 100 moves upward under the pushing action of the cell fluid, and is finally discharged through the sterile filter. The sterile filter can prevent the air with bacteria from the outside from entering the inside of the bag-connecting packaging bag 100, thereby avoiding the contamination of the cell fluid in the bag-connecting packaging bag 100.

[0053] In an embodiment of the present invention, before the step of driving the drip bucket to rotate, the bag-to-bag dispensing method further includes:

[0054] The cell solution was transferred into the dropper for degassing.

[0055] Before the cell solution is input into the bag-connecting dispensing bag 100, the cell solution is input into the drip bucket for degassing to avoid bubbles in the cell solution and the pipeline. In order to achieve a better exhaust effect, the drip bucket can be placed vertically, and the cell solution flows into the drip bucket from the bottom of the drip bucket. After a small amount of liquid flows in, the bubbles in the pipeline are removed, and the bubbles rise and are discharged from the drip bucket.

[0056] In an embodiment of the present invention, the first connection portion 150 is provided with a bubble sensor or a light sensor, and the bubble sensor or the light sensor is electrically connected to the control unit.

[0057] The step of stopping the input of the cell fluid when it is determined that the bag body 110 at the head is full of the cell fluid comprises:

[0058] When the bubble sensor or the light sensor detects that the cell fluid flows through, the pump body is controlled to stop pumping the cell fluid into the linked bag dispensing bag 100 .

[0059] The bubble sensor or the light sensor is electrically connected to the control unit, and the control unit is electrically connected to the pump body. When the bag-in-a-bag dispensing bag 100 is full of cell fluid, the cell fluid will enter the first connecting portion 150. At this time, when the bubble sensor or the light sensor detects that the cell fluid flows through, the bubble sensor or the light sensor will output an electrical signal to the control unit, indicating that the bag-in-a-bag dispensing bag 100 is full of cell fluid. The control unit recognizes the electrical signal and controls the pump body to stop working, and the pump body stops pumping the cell fluid into the bag-in-a-bag dispensing bag 100.

[0060] In an embodiment of the present invention, the first pipeline is connected in series with a pressure sensor or a flow sensor; the step of stopping the input of the cell fluid when it is determined that the bag body 110 at the head is full of cell fluid includes:

[0061] When the pressure of the first pipeline is greater than or equal to a preset pressure value, or when the flow rate of the first pipeline is greater than or equal to a preset flow rate, the pump body is controlled to stop pumping the cell fluid into the linked bag dispensing bag 100 .

[0062] The pressure sensor or flow sensor is electrically connected to the control unit. The pressure sensor is used to detect the pressure in the first pipeline. Since the bag-in-a-bag dispensing bag 100 is arranged vertically, the pressure in the first pipeline is related to the volume of the cell fluid in the bag-in-a-bag dispensing bag 100. Specifically, the higher the height of the cell fluid in the bag-in-a-bag dispensing bag 100, the greater the pressure in the first pipeline. The control unit obtains the pressure value detected by the pressure sensor in real time, and compares the pressure value with the preset pressure value. When the pressure value of the first pipeline is greater than or equal to the preset pressure value, it means that the bag-in-a-bag dispensing bag 100 is full of cell fluid. The control unit controls the pump body to stop working, and the pump body stops pumping cell fluid into the bag-in-a-bag dispensing bag 100.

[0063] It should be noted here that the pressure sensor is not only arranged in the first pipeline, but can also be arranged in the second connecting part.

[0064] The flow sensor is used to detect the flow rate of the cell sap flowing through the first pipeline. When the model of the bag-in-a-bag dispensing bag 100 is known, the capacity of the bag-in-a-bag dispensing bag 100 is also known. Therefore, by detecting the flow rate of the cell sap flowing through the first pipeline by the flow sensor, the capacity of the cell sap in the bag-in-a-bag dispensing bag 100 can be calculated. When the flow rate of the first pipeline is greater than or equal to the preset flow rate, it means that the bag-in-a-bag dispensing bag 100 is full of cell sap, and the control unit controls the pump body to stop working, and the pump body stops pumping cell sap into the bag-in-a-bag dispensing bag 100.

[0065] It should be noted here that the flow sensor is not only arranged in the first pipeline, but can also be arranged in the second connecting part.

[0066] In an embodiment of the present invention, the bag-to-bag dispensing bag 100 is connected to a weighing sensor, and the step of stopping the input of cell fluid when it is determined that the bag body 110 at the head is full of cell fluid includes:

[0067] Determine the weight of the cell solution in the connected bag dispensing bag 100 according to the weight of the connected bag dispensing bag 100 and the total weight of the connected bag dispensing bag 100 after the cell solution is input;

[0068] The weighing sensor is hooked and connected with the bag-connecting dispensing bag 100. In order to facilitate the fixation of the bag-connecting dispensing bag 100, a hanging part is provided on the bag-connecting dispensing bag 100. The weighing sensor is hooked and connected with the hanging part. The hanging part can be a through hole or a hanging ring.

[0069] Before the cell fluid is input into the bag-connecting dispensing bag 100, the weight detected by the weighing sensor is the weight of the bag-connecting dispensing bag 100. Of course, when the specifications of the bag-connecting dispensing bag 100 are known, the weight of the bag-connecting dispensing bag 100 is known, and the weight of the bag-connecting dispensing bag 100 can be taken as a known value. In the process of continuously inputting the cell fluid into the bag-connecting dispensing bag 100, the weight detected by the weighing sensor increases as the volume of the cell fluid increases. At this time, the weight detected by the weighing sensor is the total weight of the bag-connecting dispensing bag 100 after the cell fluid is input, that is, the sum of the weight of the cell fluid and the weight of the bag-connecting dispensing bag 100. The weight of the cell fluid in the bag-connecting dispensing bag 100 can be obtained by subtracting the weight of the bag-connecting dispensing bag 100 from the total weight of the bag-connecting dispensing bag 100 after the cell fluid is input into the bag-connecting dispensing bag 100.

[0070] Determine the volume of the cell fluid in the bag-connecting dispensing bag 100 according to the weight of the cell fluid in the bag-connecting dispensing bag 100;

[0071] Since the density of the cell fluid is known, after the weight of the cell fluid in the bag-in-a-bag dispensing bag 100 is obtained, the volume of the cell fluid in the bag-in-a-bag dispensing bag 100 can be calculated.

[0072] When the cell fluid volume in the bag-in-a-bag dispensing bag 100 is greater than or equal to the preset volume, the pump body is controlled to stop pumping the cell fluid into the bag-in-a-bag dispensing bag 100 .

[0073] When the cell fluid capacity in the bag-with-bag dispensing bag 100 is greater than or equal to the preset capacity, it means that the bag-with-bag dispensing bag 100 is full of cell fluid, and the control unit controls the pump body to stop working, and the pump body stops pumping cell fluid into the bag-with-bag dispensing bag 100.

[0074] In an embodiment of the present invention, after the step of heat-sealing the head end 130 and the tail end 140 of each bag body 110 by a heat sealer, the bag-to-bag packaging method further includes:

[0075] Put the connected bag 100 into a freezing device for freezing;

[0076] Placing the connected bag-packing bag 100 into a freezing device for freezing can ensure that the cell characteristics of the cell fluid are preserved, and the frozen connected bag-packing bag 100 is kept for subsequent use.

[0077] Combine the following Figure 2 Describe a specific embodiment of the present invention, Figure 2 The second flow chart of the method for packaging the connected bag packaging bag provided by the embodiment of the present invention is illustrated as follows: Figure 2 As shown, the packaging method of the bag-in-a-bag includes:

[0078] Step S100, inputting the cell fluid into the dripping funnel for degassing;

[0079] Step S200, driving the drip bucket to rotate so that the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet, so that the cell fluid in the drip bucket is input into the bag-connecting dispensing bag 100 through the second connecting portion 160;

[0080] Step S300, injecting cell fluid into the inside of the linked bag-packing bag 100 through the second connecting part 160, so that the cell fluid fills each bag body 110 from bottom to top;

[0081] Step S400, when the bubble sensor or the light sensor detects that the cell fluid is flowing, the pump body is controlled to stop pumping the cell fluid into the bag-connecting dispensing bag 100;

[0082] Step S500, heat-sealing the head end 130 and the tail end 140 of each bag body 110 by a heat-sealing device, so that a predetermined volume of cell fluid is filled in each bag body 110;

[0083] Step S600, placing the linked bag-packing bag 100 into a freezing device for freezing.

[0084] Combine the following Figure 3 Describe a specific embodiment of the present invention, Figure 3 The third flow chart of the method for packaging the connected bag packaging bag provided by the embodiment of the present invention is illustrated, as shown in FIG. Figure 3 As shown, the packaging method of the bag-in-a-bag includes:

[0085] Step S100, inputting the cell fluid into the dripping funnel for degassing;

[0086] Step S200, driving the drip bucket to rotate so that the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet, so that the cell fluid in the drip bucket is input into the bag-connecting dispensing bag 100 through the second connecting portion 160;

[0087] Step S300, injecting cell fluid into the inside of the linked bag-packing bag 100 through the second connecting part 160, so that the cell fluid fills each bag body 110 from bottom to top;

[0088] Step S400, when the pressure of the first pipeline is greater than or equal to a preset pressure value, or when the flow rate of the first pipeline is greater than or equal to a preset flow rate, the pump body is controlled to stop pumping the cell solution into the linked bag dispensing bag 100;

[0089] Step S500, heat-sealing the head end 130 and the tail end 140 of each bag body 110 by a heat-sealing device, so that a predetermined volume of cell fluid is filled in each bag body 110;

[0090] Step S600, placing the linked bag-packing bag 100 into a freezing device for freezing.

[0091] Combine the following Figure 4Describe a specific embodiment of the present invention, Figure 4 The fourth flow chart of the method for packaging the connected bag packaging bag provided by the embodiment of the present invention is illustrated, as shown in FIG. Figure 4 As shown, the packaging method of the bag-in-a-bag includes:

[0092] Step S100, inputting the cell fluid into the dripping funnel for degassing;

[0093] Step S200, driving the drip bucket to rotate so that the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet, so that the cell fluid in the drip bucket is input into the bag-connecting dispensing bag 100 through the second connecting portion 160;

[0094] Step S300, injecting cell fluid into the inside of the linked bag-packing bag 100 through the second connecting part 160, so that the cell fluid fills each bag body 110 from bottom to top;

[0095] Step S400, determining the weight of the cell fluid in the connected bag dispensing bag 100 according to the weight of the connected bag dispensing bag 100 and the total weight of the connected bag dispensing bag 100 after the cell fluid is input;

[0096] Step S500, determining the volume of the cell fluid in the bag-connecting dispensing bag 100 according to the weight of the cell fluid in the bag-connecting dispensing bag 100;

[0097] Step S600, when the cell fluid volume in the bag-in-a-bag dispensing bag 100 is greater than or equal to the preset volume, the pump body is controlled to stop pumping the cell fluid into the bag-in-a-bag dispensing bag 100;

[0098] Step S700, heat-sealing the head end 130 and the tail end 140 of each bag body 110 by a heat-sealing device, so that a predetermined volume of cell fluid is filled in each bag body 110;

[0099] Step S800, placing the linked bag-packing bag 100 into a freezing device for freezing.

[0100] The packaging method of the connected bag packaging bag provided in the embodiment of the present invention adopts a serially designed connected bag packaging bag to store cell fluid, and inputs the cell fluid into the bag body from bottom to top, thereby realizing the emptying of bubbles, and can achieve precise preparation of the minimum unit volume, without the need for manual preparation in a sterile environment, thus meeting the high requirements of precise preparation, having the effect of portable packaging, and effectively reducing labor costs.

[0101] Figure 5 The schematic diagram of the side cross-sectional structure of the linked bag dispensing bag provided by the embodiment of the present invention is illustrated as follows: Figure 5As shown, the second aspect of the present invention further provides a connected bag-packing bag 100, which is based on the packaging method of the connected bag-packing bag described in any one of the above embodiments. The connected bag-packing bag 100 includes a plurality of bag bodies 110, and the plurality of bag bodies 110 are connected end to end in sequence, that is, the head end 130 of the previous bag body 110 is connected to the tail end 140 of the next bag body 110, and a connecting channel 120 for connecting the two adjacent bag bodies 110 is formed at the connection between the two adjacent bag bodies 110, and a first connecting portion 150 is provided at the head bag body 110, and the first connecting portion 150 is connected to the head bag body 110, and a second connecting portion 160 is provided at the tail bag body 110, and the second connecting portion 160 is connected to the tail bag body 110.

[0102] It should be noted that the connection channel 120 between two adjacent bag bodies 110 can have various structural forms. The connection channel 120 can be a flow channel formed at the connection between two adjacent bag bodies 110, or can be a pipeline arranged between two adjacent bag bodies 110.

[0103] In an embodiment of the present invention, the capacity of each bag body 110 is equal, so that after the head end 130 and the tail end 140 of each bag body 110 are heat-sealed, the cell fluid capacity in each bag body 110 is equal. Of course, the capacity of the bag body 110 can also be different, for example, the capacity of a part of the bag body 110 is 10ml, and the capacity of the other part of the bag body 110 is 15ml. After packaging, cell preparation bags of different specifications can be obtained. When the capacity of the bag body 110 is different, and the capacity of different bag bodies 110 is close, it is difficult to judge the capacity of the bag body 110 by observing the size of the bag body 110. For example, the capacity of a part of the bag body is 11ml, and the capacity of the other part of the bag body is 10ml. The two are mixed, and it is difficult to directly judge the capacity of the bag body 110. In order to facilitate the judgment of the capacity of the bag body 110, a mark can be set on the surface of the bag body 110 for identification, and the mark can be a number or a symbol of different colors.

[0104] In the embodiment of the present invention, the plurality of bag bodies 110 are on the same straight line, the plurality of connecting channels 120 are also on the same straight line, and the shapes of the bag bodies 110 are the same. The cross section of the head end 130 of each bag body gradually decreases along the first direction, and the cross section of the tail end 140 of the bag body gradually decreases along the second direction, and the first direction is opposite to the second direction. The first direction refers to the direction from the tail end 140 to the head end 130, and the second direction refers to the direction from the head end 130 to the tail end 140. Figure 5As shown, the cross section of the head end 130 of each bag body 110 gradually decreases in the left direction, and the cross section of the tail end 140 of the bag body 110 gradually decreases in the right direction. Since the cross section of the head end 130 and the cross section of the tail end 140 of each bag body 110 gradually decrease in opposite directions, the connection between two adjacent bag bodies 110 shrinks inwards, that is, the inner walls of the connection between two adjacent bag bodies 110 are gathered inwards, and the connection between two adjacent bag bodies 110 forms a V-shaped connection structure, which can facilitate the heat sealing of the bag body 110, avoiding the deviation of the heat sealing position, resulting in deviation of the capacity of the bag body 110.

[0105] It should be noted that the cross section of the bag body 110 refers to the cross section of the bag body 110 along the Figure 5 The longitudinal section of the bag body 110 refers to the section made along the front-to-back direction. Figure 5 Cross section in the left-right direction.

[0106] Furthermore, in order to prevent the actual heat-sealing position from deviating from the designed heat-sealing position during the heat-sealing process of the bag body 110, an identification line or identification area can be set at the connection between two adjacent bag bodies 110. During the heat-sealing process of the bag body 110, as long as the heat sealer is aligned with the identification line or identification area, the bag body 110 can be accurately packaged, thereby improving the packaging accuracy of the bag body 110.

[0107] In an embodiment of the present invention, the cross-sectional shape of each bag body 110 is the same, and the longitudinal cross-sectional shape of each bag body 110 is the same. The longitudinal cross-sectional shape of the bag body 110 can be a rhombus, a sphere, a regular hexagon or other shapes. Since the cross-sectional shape of the head end 130 of each bag body 110 gradually decreases, during the process of the cell fluid rising in the bag-connecting and packaging bag 100, the bubbles in the bag body 110 have no place to hide and can only be pushed upward by the cell fluid and finally discharged through the sterile filter. Compared with the bag body 110 with a flat head end 130, the bag body 110 of the present invention has a better exhaust effect.

[0108] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for packaging a continuous bag, characterized in that: include: Inputting cell fluid into the interior of the linked bag dispensing bag (100) through the second connecting portion (160) so that the cell fluid fills each bag body (110) from bottom to top; When it is determined that the bag body (110) at the front is full of cell fluid, the input of cell fluid is stopped; The head end (130) and the tail end (140) of each bag body (110) are heat-sealed by a heat-sealing device, so that a predetermined volume of cell fluid is contained in each bag body (110).

2. The method for packaging a continuous bag-packing bag according to claim 1, characterized in that: The liquid outlet of the drip bucket is connected to the second connection part (160) through the first pipeline. Before the step of inputting the cell solution into the inside of the bag-connecting dispensing bag (100) through the second connection part (160), the step further includes: The drip bucket is driven to rotate so that the height of the drip bucket liquid inlet is greater than the height of the drip bucket liquid outlet, thereby allowing the cell fluid in the drip bucket to be input into the interior of the linked bag dispensing bag (100) through the second connecting portion (160).

3. The method for dispensing the linked bag dispensing bag according to claim 2, characterized in that: Before the step of driving the drip bucket to rotate, the method further includes: The cell solution is input into the dropper for degassing.

4. The method for packaging a continuous bag-packing bag according to any one of claims 1 to 3, characterized in that: The first connection portion is provided with a bubble sensor or a light sensor; the step of stopping the input of cell fluid when it is determined that the bag body (110) located at the head portion is full of cell fluid comprises: When the bubble sensor or the light sensor detects the cell fluid flowing through, the pump body is controlled to stop pumping the cell fluid into the linked bag dispensing bag (100).

5. The method for packaging a linked bag packing bag according to claim 2 or 3, characterized in that: The first pipeline is serially connected with a pressure sensor or a flow sensor; the step of stopping the input of cell fluid when it is determined that the bag body (110) located at the head is full of cell fluid comprises: When the pressure of the first pipeline is greater than or equal to a preset pressure value, or when the flow rate of the first pipeline is greater than or equal to a preset flow rate, the pump body is controlled to stop pumping cell fluid into the linked bag dispensing bag (100).

6. The method for packaging a continuous bag-packing bag according to any one of claims 1 to 3, characterized in that: The bag-connecting dispensing bag (100) is connected to a weighing sensor, and the step of stopping the input of cell fluid when it is determined that the bag body (110) located at the head is full of cell fluid comprises: Determining the weight of the cell fluid in the connected bag dispensing bag (100) according to the weight of the connected bag dispensing bag (100) and the total weight of the connected bag dispensing bag (100) after the cell fluid is input; Determining the volume of the cell fluid in the linked bag dispensing bag (100) according to the weight of the cell fluid in the linked bag dispensing bag (100); When the cell fluid volume in the bag-linked dispensing bag (100) is greater than or equal to a preset volume, the pump body is controlled to stop pumping the cell fluid into the bag-linked dispensing bag (100).

7. A linked bag dispensing bag (100), wherein the linked bag dispensing bag (100) is based on the dispensing method of the linked bag dispensing bag according to any one of claims 1 to 6, characterized in that: include: A plurality of bag bodies (110) are connected end to end in sequence, a connection passage (120) for connecting the two adjacent bag bodies (110) is formed at the connection point of the bag body (110) located at the head portion is provided with a first connection portion, and a second connection portion (160) is provided at the bag body (110) located at the tail portion.

8. The linked bag dispensing bag according to claim 7, characterized in that: The capacity of each bag body (110) is equal.

9. The linked bag dispensing bag according to claim 7, characterized in that: The cross section of the head end (130) of each bag body (110) gradually decreases along the first direction, and the cross section of the tail end (140) of the bag body (110) gradually decreases along the second direction.

10. The linked bag dispensing bag according to claim 7, characterized in that: The first connection part is used to communicate with the sterile filter, and the second connection part (160) is used to communicate with the liquid outlet of the drip bucket or the liquid outlet of the bubble removal container through the first pipeline.

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