Composition containing biological source insecticide and application thereof

Abstract

The invention belongs to the field of pesticides, and relates to a composition containing a biological source pesticide. The composition contains an active component A and an active component B, the component A is selected from a compound I-72, and the component B is selected from biological source insecticides and acaricides; the weight part ratio of the component A to the component B is (1: 99)-(99: 1); wherein the structural formula of the compound I-72 is shown in the specification, and the composition disclosed by the invention has an obvious synergistic effect and can be used for preventing and treating various pests and pest mites. # imgabs0 #

Description

[0001] This application is a divisional application of the Chinese invention patent application. The original application date is November 29, 2022, application number 202211511960.5, the name of the invention: A composition containing biogenic insecticides and its application, publication number: CN118104668A; Since the original application has the unity problem pointed out by the examiner, the applicant filed a divisional application. Technical Field

[0002] The invention belongs to the field of pesticides and relates to a composition containing a biogenic insecticide and application thereof. Background Art

[0003] At present, chemical pesticides are still the main means of controlling pests and diseases, but long-term testing of chemical pesticides has serious problems such as pesticide residues, environmental pollution, pest resistance and rampant pests. Therefore, it has become an urgent task to reduce the use and gradually eliminate chemical pesticides with toxic side effects, develop non-toxic and harmless pesticides with no harmful residues or low toxicity, and explore new methods of pest control.

[0004] With the continuous development of pesticides, biopesticides have become one of the hot spots for research and development of biopesticides at home and abroad due to their advantages of high selectivity, low toxicity, easy degradation, and low resistance. However, biopesticides also have limitations such as slow action speed and unstable prevention effect. By mixing chemical pesticides and biopesticides into mixed components in a certain proportion, the prevention effect can often be improved, the amount of active ingredients can be reduced, and costs can be saved. It is also one of the effective methods for controlling pests. However, there has been no report on the composite composition of compound I-72 and biopesticides so far.

[0005] Chinese invention patent CN112457288 discloses a piperic acid derivative and its application, among which compound I-72 has an outstanding control effect on pests. Summary of the invention

[0006] The purpose of the present invention is to provide a synergistic compound composition containing biogenic insecticides and application thereof.

[0007] To achieve the above purpose, the present invention adopts the following technical solution:

[0008] A composition containing a biogenic insecticide, the composition comprises two active components A and B, component A is selected from a piperic acid derivative compound of the following structure, and component B is selected from a biogenic insecticide and acaricide; the weight ratio of the two active components A and B is 1:99 to 99:1; wherein the piperic acid derivative compound has the following structural formula:

[0009]

[0010] The active component B is selected from microbial preparations, matrine, eucalyptol, mineral oil, star anise oil, allicin, ginkgo phenol, d-limonene, diatomaceous earth, rotenone, azadirachtin, celandine extract, cordyceps sinensis, Veratrum rhizome extract, Paecilomyces cerevisiae, Aspergillus niger, Verticillium truncatum, osthole, wolfsbane, tea saponin, oxymatrine, preparations containing nuclear polyhedrosis virus, preparations containing cytoplasmic polyhedrosis virus, preparations containing granular virus, quinoa or coronatine.

[0011] The microorganisms in the microbial preparation contained in the active component B are Bacillus thuringiensis, Bacillus thuringiensis israelii, methylotrophic Bacillus 9912, methylotrophic Bacillus LW-6, marine Bacillus, spherical Bacillus, Bacillus subtilis, Japanese beetle Bacillus, polymyxa Bacillus, Brevibacillus laterosporus A60, Bacillus brevis, Bacillus licheniformis, Bacillus amyloliquefaciens B1619, Bacillus amyloliquefaciens B7900, Bacillus amyloliquefaciens B1619, Bacillus amyloliquefaciens PQ21, Bacillus amyloliquefaciens LX-11, Bacillus amyloliquefaciens AT-332, Rhodopseudomonas palustris PSR-S, Bacillus thuringiensis G033A, Metarhizium anisopliae, Beauveria bassiana, Metarhizium anisopliae, Ear mold, Beauveria bassiana or Penicillium sphaerocephala;

[0012] The nuclear polyhedrosis virus in the preparation containing nuclear polyhedrosis virus in the active component B is Spodoptera californica nuclear polyhedrosis virus, Spodoptera brassicae nuclear polyhedrosis virus, Spodoptera exigua nuclear polyhedrosis virus, Spodoptera litura nuclear polyhedrosis virus, Helicoverpa armigera nuclear polyhedrosis virus, Tea geometrid nuclear polyhedrosis virus or Spodoptera mulberry nuclear polyhedrosis virus;

[0013] The cytoplasmic polyhedrosis virus in the preparation containing cytoplasmic polyhedrosis virus is pine caterpillar cytoplasmic polyhedrosis virus;

[0014] The granular virus in the preparation containing granular virus is Pieris rapae granular virus, Pieris rapae granular virus, Spodoptera exigua granular virus, Pieris rapae granular virus, Plutella xylostella granular virus, Tea leaf roller granular virus, Armyworm granular virus or Rice leaf folder granular virus.

[0015] Component A in the composition is selected from piperic acid derivative compounds; component B is selected from Bacillus thuringiensis, matrine, d-limonene, rotenone, mineral oil, azadirachtin, green anisopliae, Beauveria bassiana, Veratrum rhizome extract, osthole, a preparation containing Spodoptera exigua nuclear polyhedrosis virus, and a preparation containing Spodoptera exigua nuclear polyhedrosis virus; the weight ratio of the two active components A and B is 1:60 to 60:1.

[0016] Component A in the composition is selected from piperic acid derivative compounds; component B is selected from matrine, d-limonene, rotenone, mineral oil, and azadirachtin; and the weight ratio of the two active components A and B is 1:20 to 60:1.

[0017] Component A in the composition is selected from piperic acid derivative compounds; component B is selected from matrine, d-limonene, and rotenone; and the weight ratio of the two active components of component A and component B is 1:10 to 10:1.

[0018] In the composition, component A is selected from piperic acid derivative compounds; component B is selected from azadirachtin; and the weight ratio of the two active components, component A and component B, is 1:20 to 20:1.

[0019] The application of the composition is to prepare insecticides and miticides for preventing and controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, and various plant diseases.

[0020] The present invention also includes the use of the inventive composition to prepare an insecticide and acaricide preparation for controlling agricultural and forestry pests. Specifically, it is suitable for the prevention and control of a variety of important agricultural pests, such as aphids, diamondback moths, armyworms, cotton bollworms, beet armyworms, armyworms, cabbage armyworms, American white moths, cutworms, geometrids, peach borers, pear borers, fall armyworms, tobacco budworms, geometrids, rice leaf rollers, corn borers, striped stem borers, yellow stem borers, grass borers, bean pod borers, peach borers, citrus leaf miners, grape codling moths, tussock moths, caterpillars, tea geometrids, tea caterpillars, leafhoppers, flower scale insects, potato beetles, wireworms, grubs, flea beetles, leaf beetles, weevils, pine caterpillars, underground pests, nematodes, locusts, western flower thrips, palm thrips, onion thrips, rice thrips, ginger thrips, mango thrips, whiteflies, gall midges, blind bugs, etc.

[0021] In particular, the insecticidal and acaricidal composition of the present invention can be used to control pests of the order Thysanoptera, and non-exhaustive examples of specific species include but are not limited to rice thrips, tobacco thrips, rice pipe thrips, wheat skin thrips, end-band thrips, tobacco thrips, flower thrips, yellow thrips, sugarcane thrips, cucurbit thrips, western flower thrips, palm thrips, etc.; the combination can be used to control pests of the order Homoptera, and non-exhaustive examples of specific species include but are not limited to species of the genus Aleuroptera: greenhouse whitefly, tobacco whitefly, silverleaf whitefly, sweet potato whitefly, cabbage whitefly, cabbage whitefly, spiral whitefly, soft-haired whitefly, cotton whitefly, lemon fruit brown leafhopper, mango leafhopper, long foam cicada species, foam cicada; gray planthopper, small brown planthopper, rice brown planthopper, brown planthopper, corn planthopper, corn flower-winged planthopper, White-backed rice hopper, white-backed planthopper; aster leafhopper, aster leafhopper, raspberry leafhopper, black-tailed leafhopper species, leafhopper, black-tailed leafhopper, large green leafhopper, white-winged leafhopper, large green leafhopper, cotton leafhopper, elm leafhopper, small green leafhopper, Indian cotton leafhopper, spotted wax cicada, etc.; the combination can be used to control pests of Hemiptera, and non-exhaustive examples of specific species include but are not limited to pear web bugs, banana web bugs, fine-horned flower bugs, tiny flower bugs, needle-edged bugs, large rice-edged bugs, rice spider bugs, rice brown bugs, rice black bugs, rice green bugs, green stink bugs, alfalfa stink bugs, middle black stink bugs, wheat root stink bugs, three-spotted stink bugs, pasture stink bugs, litchi stink bugs; the combination can be used to control pests of Lepidoptera, and non-exhaustive examples of specific species include but are not limited to grain moths, clothes moths, yellow thorn moths, brown thorn moths, flat thorn moth, wheat moth, cotton bollworm, tobacco budworm, sweet potato moth, diamondback moth, soybean borer, peach borer, hemp borer, pear borer, pear borer, codling moth, apple leaf roller, apple leaf roller, apple leaf roller, yellow leaf roller, apple nest moth, brown-banded leaf roller, yellow leaf roller, black-spotted brown leaf roller, litchi butterfly, dimorphic Stem borer, bean pod borer, corn borer, yellow stem borer, Taiwan rice borer, cabbage borer, rice leaf roller, millet ash borer, duckweed ash borer, duckweed borer, stripe borer, cotton leaf roller, sweet potato stem borer, one-spotted grain borer, peach borer, sunflower borer, yellow stem borer, two-spotted stem borer, white stem borer, white-belt stem borer, grass borer, armyworm, armyworm, silver-striped armyworm, pit-wing armyworm, quinoa armyworm, jute armyworm, ramie armyworm, mouth kettle armyworm moth, mango horizontal line tail armyworm, cabbage armyworm, beet armyworm, show armyworm, loquat yellow caterpillar, ramie red butterfly, ramie yellow butterfly, rice stem borer, cotton small bridge borer, large borer, cotton bollworm, cotton leaf roller, soybean leaf roller, peach borer, Dingdian diamond, emerald diamond, Egyptian diamond, small cutworm, large cutworm, yellow cutworm, white-edged cutworm, warning-marked cutworm, The thief moth, gypsy moth, sweet potato hawkmoth, bean hawkmoth, straight-striped rice hesperid, hidden-striped rice hesperid, citrus swallowtail, jade-banded swallowtail, cabbage butterfly, double-banded night butterfly, rice leaf night butterfly, powdery moth, tea caterpillar, geometrid, tea moth, big moth, small moth, tea tip moth, tea branch sickle moth, wild silkworm, mulberry caterpillar, tent caterpillar, walnut moth, persimmon calyx moth, citrus leaf miner, banana hesperid;The combination can be used to control pests of the order Coleoptera, and non-exhaustive examples of specific species include, but are not limited to, bean lycopersicon, yellow mealworm, black mealworm, red flour beetle, mixed flour beetle, large flour beetle, saw-shaped flour beetle, long-horned flat flour beetle, black wrinkled gill beetle, clouded gill beetle, yellow-brown beautiful beetle, copper-green beautiful beetle, dark beetle, North China large black gill beetle, mulberry longhorn beetle, star longhorn beetle, orange brown longhorn beetle, peach red neck longhorn beetle, red neck longhorn beetle, sugarcane saw-shaped longhorn beetle, ramie longhorn beetle, hemp longhorn beetle, brown longhorn beetle, light shield green longhorn beetle, big monkey leafworm, small monkey leafworm, rice root leaf beetle, millet scale leaf beetle, hemp leaf beetle, malignant leaf beetle, orange leaf beetle, citrus leaf beetle, yellow melon, yellow curved striped flea beetle, beet flea beetle, grain flea beetle, mung bean weevil, pea weevil, broad bean weevil, corn weevil, rice weevil, root nodule weevil, tea beautiful pattern weevil, tea Seed weevils, beet weevils, rape stem weevils, pear tigers, apricot tigers, peach tigers, black velvet beetles, net-meshed ground beetles, Mongolian ground beetles, rice weevils, rice mudworms, corn iron beetles, sweet potato green beetles, spike-backed glycoside turtles, light-backed glycoside turtles, yellow leafworms, banana weevils, banana double-black-banded weevils; the combination can be used to control pests of the order Diptera, and non-exhaustive examples of specific species include but are not limited to wheat red midges, wheat yellow midges, rice gall midges, rice chironomids, rice stalk flies, rice leaf miners, rice water flies, citrus fruit flies, citrus fruit flies, citrus fruit flies, melon fruit flies, wheat leaf gray leafminers, American spotted leafminers, bean stalk black leafminers, wheat stalk flies, seed flies, onion flies, radish flies, sorghum awn flies, millet stalk flies, astragalus leafminers, duckweed chironomids, beet leafminers, beet root maggots, and radish flies. Homoptera (aphids, scale insects, whiteflies, leafhoppers). A non-exhaustive list of these pests includes, but is not limited to: pea aphid, pea aphid, Aphid spp., Aphid spp., Aphid spp., potato aphid, potato aphid, wheat aphid, English wheat aphid, rose aphid, rose aphid, Aphid spp., cotton aphid, eggplant aphid, foxglove aphid, wheat aphid, Russian aphid, asparagus aphid, asparagus aphid, plantago aphid, apple aphid, woolly apple aphid. , apple aphid, wheat aphid, rose wheat aphid, peach aphid, green peach aphid, grape phylloxera, grape phylloxera, cabbage aphid, cabbage aphid, Aphis spp., aphid, corn leaf aphid, corn aphid, cereal aphid, wheat aphid, green aphid; Whitefly spp., whitefly, silverleaf whitefly, Bemisia tabaci, sweet potato whitefly, cabbage whitefly, cabbage whitefly, spiral whitefly, soft-haired whitefly, cotton whitefly;Species of the genus Aphrophora, Amrasca bigutellabigutella, Species of the genus Aphrophora, Leafhoppers, Orange Round Kidney Scale, Orange Round Scale, Rice Flour Scale, Species of the genus Snow Shield, Scale Insects, Red Wax Scale, Red Wax Scale, Species of the genus Snow Shield, Scale Insects, Species of the genus Brown Round Shield, Scale Insects, Species of the genus Soft Scale, Scale Insects, Blowing Scale, Blowing Scale, Species of the genus Oyster Shield, Bran Flake Scale, Bran Flake Scale, Black Shield scale, ebony scale, spruce ball scale, spruce round shield scale, mealybug species, mealybug, mealybug species, mealybug, pineapple mealybug, pineapple mealybug, pear round scale, St. Joseph scale, black helmet scale species, scale insects, olive black helmet scale, black scale insects, arrow scale species, scale insects, arrow scale, arrow scale, and Zulia entreriana. ;

[0022] The composition of the present invention is applicable to, but not limited to, the following crops or plants: cereal crops, such as wheat, barley, rye, oats, rice, corn, sorghum, millet, etc.; tuber crops: including sweet potato, yam, potato, etc.; legume crops: including soybean, broad bean, pea, mung bean, etc.; fiber crops: including cotton, jute, kenaf, hemp, flax, sisal, mat grass, etc.; oil crops: including oilseed, rapan, mustard, peanut, sesame, hemp, sunflower, etc.; Sugar crops: including sugar cane, sugar beet, etc.; beverage crops: including tea, coffee, cocoa, etc.; dye crops: including indigo, saffron, etc.; spice crops: including lemongrass, mint, rose; fodder crops: including alfalfa, sweet clover, astragalus, vetch, clover, etc.; fodder crops: including ryegrass, oat grass, Sudan grass, etc.; green manure crops, such as vetch, astragalus, yellow alfalfa, sweet clover, tamarind, sesbania, amorpha, etc.; fruits, such as apples, pear, plum, peach, almond, cherry, citrus, mango, litchi, banana, grape; or berry, such as strawberry, raspberry or blackberry mustard, poppy, olive, coconut, castor, etc.; vegetable, such as cucumber, loofah, watermelon, melon, pumpkin, hanging melon, spinach, celery, kale, cabbage, cauliflower, gourd, pepper, eggplant, tomato, onion, ginger, garlic, leek, lettuce, kidney bean, cowpea, radish, carrot, yam, etc.; the composition of the present invention can also be used as a For any ornamental plants, including flowers, shrubs, broadleaf trees and evergreens, such as violets, begonias, dahlias, gerberas, hydrangeas, verbenas, roses, kalanchoe, poinsettias, aster, cornflowers, coreopsis, delphiniums, monopetalum, phlox, rhodiola, sedum, petunias, violas, impatiens, geraniums, chrysanthemums, ranunculus, fuchsias, sage, wild woodruff, rosemary, sage, St. John's wort, mint. When used to control urban pests, it can be used in homes, various public places, offices, and trees, etc.

[0023] The technical solution also includes the use of the insecticide and acaricide composition of the present invention in preparing agricultural insecticide and acaricide drugs.

[0024] The insecticide and acaricide composition of the present invention can be applied at an effective dose to the pests / mites to be controlled and / or their habitats.

[0025] The advantages of the present invention are as follows: the binary composition obtained by compounding compound I-72 with a biogenic insecticide in an appropriate ratio has a good synergistic effect, thereby improving the control effect, reducing the dosage, and expanding the insecticide spectrum. Specifically,

[0026] 1. The two active components A and B in the compound have different mechanisms of action, which can delay the development of resistance of pests and mites. 2. The insecticide spectrum has been expanded, and it can be used to control a variety of pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton and other crops; 3. The amount of pesticides used is reduced, the amount of pesticide residues on crops is reduced, and the friendliness to the environment is enhanced. 4. The two active components A and B are compounded within an appropriate ratio range, which improves the control effect of pests and mites such as the striped stem borer, rice brown planthopper, thrips, and peach aphid. DETAILED DESCRIPTION

[0027] The following examples are used to explain the present invention in detail, so as to facilitate further understanding of the present invention. The active component A is selected from compound I-72 (prepared according to the description in the patent document CN112457288, with a content of 97%); the active component B and other raw materials are all commercially available products. The active components in the formula examples are metered and added after 100%, and each component is measured by weight percentage.

[0028] Indoor Activity Assay Example

[0029] Example 1: Indoor joint toxicity test of compound I-72 and d-limonene on Bemisia tabaci

[0030] Test subjects: Bemisia tabaci (Gennadius), adults, sensitive strain reared indoors.

[0031] Preparation of drug solution: According to different test needs, use an electronic analytical balance to accurately weigh the test samples, dissolve the original drug in acetone, and then use 0.1% Tween 80 water to dilute it into a series of drug solutions with a certain concentration gradient according to the designed experimental dosage.

[0032] Test conditions: Temperature: 25-27°C, Humidity: RH 60%, Light: L:D=14:10

[0033] Test method: Use the spray method, select greenhouse bean seedlings, spray evenly in the order of low dose to high dose according to the test design, cover with homemade glass cover after natural shade drying, and fill with test insects, 20-30 heads per treatment, 3 replicates, and set up a blank control. Investigate the number of dead and alive insects after 72 hours. Use Abbott formula to calculate the corrected mortality rate, use DPS data processing software for statistical analysis, and calculate the toxicity LC of the test single agent and the test mixture at the test ratio 50 value.

[0034] Evaluation method: Sun YP method was used to calculate the co-toxicity coefficient of each mixture ratio and evaluate the type of combined action of each mixture.

[0035] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0036]

[0037] Where: ATI-actual toxicity index of the mixture; S-LC of the standard agent 50 ; LC of M-mixture 50 .

[0038] TTI=TIA×PA+TIB×PB

[0039] In the formula: TTI-theoretical toxicity index of mixture; TIA-toxicity index of A agent; percentage of PA-A agent in the mixture; TIB-toxicity index of B agent; percentage of PB-B agent in the mixture.

[0040]

[0041] Where: CTC-co-toxicity coefficient; ATI-actual toxicity index of mixture; TTI-theoretical toxicity index of mixture.

[0042] The co-toxicity coefficient (CTC) of the compound agent ≥120 shows a synergistic effect; CTC ≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0043] As can be seen from the table (Table 1), compound I-72 and d-limonene mixed at a ratio of 10:1-1:10 have a significant synergistic effect on adults of Bemisia tabaci.

[0044] Table 1 Results of indoor combined toxicity test of compound I-72 and d-limonene against Bemisia tabaci adults

[0045]

[0046] Example 2: Indoor combined toxicity test of compound I-72 and matrine against green peach aphid

[0047] Test subjects: Myzus persicae, a mixed aphid, a sensitive strain reared indoors.

[0048] Test method: Use the insect leaf spray method. Take cabbage leaves with a certain number of peach aphids, and spray the prepared solution evenly on the front and back of the leaves in the order of the experimental design concentration from low to high. Place it in a culture dish with a filter paper with a diameter of 6 cm. After natural drying, place it in an observation room. The test is repeated 3 times, and a blank control is set up. The number of dead and living insects is investigated after 48 hours. The Abbott formula is used to calculate the corrected mortality rate, and the DPS data processing software is used for statistical analysis to calculate the toxicity LC of the test single agent and the test ratio of the mixed agent. 50 value.

[0049] The test conditions, solution preparation and evaluation method are the same as those in Example 1.

[0050] As can be seen from the table (Table 2), compound I-72 and matrine mixed at a ratio of 10:1-1:10 have a significant synergistic effect on green peach aphids.

[0051] Table 2 Results of indoor combined toxicity test of compound I-72 and matrine against aphids

[0052]

[0053] Example 3: Indoor joint toxicity test of compound I-72 and rotenone mixed with yellow flea beetle

[0054] Test target: Yellow-striped flea beetle Phyllotreta striolata (Walker), adult indoor rearing strain.

[0055] Test method: Use the immersion method. Select adult flea beetles of the same size and put them into the immersion cage. According to the test design, immerse them for 10 seconds in the order from low dose to high dose. 30 heads are placed in a 9 cm diameter culture dish with filter paper. Each treatment is repeated 3 times, and a blank control is set. The treated test materials are placed in an observation room, and the temperature, humidity and light of the observation room can be adjusted as needed.

[0056] The test conditions, solution preparation and evaluation method are the same as those in Example 1.

[0057] As can be seen from the table (Table 3), compound I-72 and rotenone mixed at a ratio of 1:10-10:1 showed a synergistic effect on the yellow flea beetle.

[0058] Table 3 Results of indoor combined toxicity test of compound I-72 and rotenone on yellow flea beetle

[0059]

[0060] Example 4: Indoor combined toxicity test of compound I-72 and azadirachtin against Chilo suppressalis

[0061] Test target: Chilo suppressalis (Walker), 2nd instar larvae, collected from Nanchang, Jiangxi Province, 11th generation of indoor continuous culture, with high resistance to amides such as chlorantraniliprole.

[0062] Test method: Spray method was used. Rice stalks of uniform length and thickness were selected, cut to the same length, and sprayed evenly in the order from low dose to high dose according to the test design. 1.5 mL of the solution was placed in a culture dish with a diameter of 6 cm and filter paper for each treatment. After natural drying, neat healthy test insects were inoculated. Ten insects were treated for each treatment, and four replicates were performed for each treatment. A blank control was also set up. The number of dead and live insects was investigated after 72 hours. The adjusted mortality rate was calculated using the Abbott formula, and statistical analysis was performed using the DPS data processing software to calculate the toxicity LC of the test single agent and the test mixture at the test ratio. 50 value.

[0063] The test conditions, solution preparation and evaluation method are the same as those in Example 1.

[0064] As can be seen from the table (Table 4), compound I-72 and azadirachtin mixed at a ratio of 1:20-20:1 showed a synergistic effect against western flower thrips.

[0065] Table 4 Results of indoor combined toxicity test of compound I-72 and azadirachtin against Chilo suppressalis

[0066]

[0067] Field test example

[0068] Example 5: Field efficacy test of compound I-72 and Bacillus thuringiensis against rice leaf folder

[0069] This experiment was arranged in the suburbs of Jurong City, Jiangsu Province. The conventional spraying method was used. The survey method adopted 5-point sampling and a total of 25 rice clumps were surveyed. The survey was conducted 20 days after the application of the pesticide. The leaf curling rate was compared with that of the control area, and the relative control effect was calculated. The water consumption per mu was 45L.

[0070] The test agents were A: 8000 IU / μL Bacillus thuringiensis suspension (Qingdao, Shandong Audis Biotechnology Co., Ltd.), B: compound I-72 (dissolved in acetone, added with 0.1% Tween 80, and prepared into 10% stock solution for use).

[0071] Leaf curling rate = number of curled leaves surveyed / total number of leaves surveyed*100

[0072] Control effect = (CK-PT) / CK*100

[0073] Where: CK is the leaf curling rate of the blank control area after drug application

[0074] PT is the leaf curling rate after drug treatment in the area

[0075] As can be seen from the table (Table 5), the combination of compound I-72 and Bacillus thuringiensis is significantly better than the use of a single agent in controlling rice leaf folder.

[0076] Table 5 Field efficacy test results of compound I-72 Bacillus thuringiensis against rice leaf folder

[0077]

[0078] Example 6: Field efficacy test of compound I-72 and beet armyworm nuclear polyhedrosis virus against beet armyworm

[0079] This experiment was arranged in the field test shed area of ​​Wuhan Vegetable Research Institute. The spraying method was used for pesticide application. The survey method adopted 5-point sampling. Two plants were fixed at each point. A total of 10 plants were surveyed in each plot. Four replicates were conducted. The insect population base was surveyed before pesticide application. Surveys were conducted 3 days and 7 days after pesticide application. The water consumption per mu was 30L. The test agent was the efficacy calculation method as follows:

[0080]

[0081] Test agents: A: 3 billion PIB beet armyworm nuclear polyhedrosis virus suspension (Henan Jiyuan Baiyun Industrial Co., Ltd.); B: compound I-72 (dissolved in acetone, added with 0.1% Tween 80, and prepared into 10% stock solution for use).

[0082] As can be seen from the table (Table 6), the combination of compound I-72 and beet armyworm nuclear polyhedrosis virus has a good control effect on beet armyworm, and the effect of combined use is significantly better than that of a single agent.

[0083] Table 6 Field efficacy test results of compound I-72 and beet armyworm nuclear polyhedrosis virus against beet armyworm

[0084]

[0085]

[0086] Example 7: Field efficacy test of compound I-72 and Beauveria bassiana against tea green leafhopper

[0087] This test was arranged to be carried out in the tea garden of Yuhang District, Hangzhou. The spraying method was adopted. The survey method adopted 5-point sampling, 2 plants were fixed at each point, and 10 plants were checked in each plot. Four times were repeated. The number of false-eyed small green leafhopper nymphs on the back of 100 tender leaves in each plot was randomly surveyed. The spraying volume was 75L / mu. The base number was surveyed before the spraying, and the number of live small green leafhoppers in each treatment sample point was surveyed 3, 7, and 14 days after the spraying. The test agent was the same as the efficacy calculation method in Example 6.

[0088] Test agents: A: 40 billion spores / g Beauveria bassiana wettable powder (Jiangxi Tianren Ecological Co., Ltd.); B: Compound I-72 (dissolved in acetone, added with 0.1% Tween 80, and prepared into 10% stock solution for use).

[0089] As can be seen from the table (Table 7), the combination of compound I-72 and Beauveria bassiana has a good control effect on tea green leafhoppers, and the control effect of the combination is significantly better than that of a single agent.

[0090] Table 7 Field efficacy test results of compound I-72 and Beauveria bassiana against tea green leafhopper

[0091]

Claims

1. A composition containing a biogenic insecticide, characterized in that: The composition comprises two active components, A and B, wherein component A is selected from the piperic acid derivative compounds of the following structure, and component B is selected from insecticides and miticides of biological origin; the weight ratio of the two active components, A and B, is 1:99 to 99:1; wherein the structural formula of the piperic acid derivative compounds is as follows: The active component B is selected from microbial preparations, matrine, eucalyptol, mineral oil, star anise oil, allicin, ginkgo phenol, diatomaceous earth, rotenone, azadirachtin, celandine extract, cordyceps sinensis, Veratrum rhizome extract, Paecilomyces cerevisiae, Aspergillus niger, Verticillium truncatum, osthole, wolfsbane, tea saponin, oxymatrine, preparations containing nuclear polyhedrosis virus, preparations containing cytoplasmic polyhedrosis virus, preparations containing granular virus, quinoa or coronatine.

2. The composition according to claim 1, characterized in that: The microorganisms in the microbial preparation contained in the active component B are Bacillus thuringiensis, Bacillus thuringiensis israelii, methylotrophic Bacillus 9912, methylotrophic Bacillus LW-6, marine Bacillus, spherical Bacillus, Bacillus subtilis, Japanese beetle Bacillus, polymyxa Bacillus, Brevibacillus laterosporus A60, Bacillus brevis, Bacillus licheniformis, Bacillus amyloliquefaciens B1619, Bacillus amyloliquefaciens B7900, Bacillus amyloliquefaciens B1619, Bacillus amyloliquefaciens PQ21, Bacillus amyloliquefaciens LX-11, Bacillus amyloliquefaciens AT-332, Rhodopseudomonas palustris PSR-S, Bacillus thuringiensis G033A, Metarhizium anisopliae, Beauveria bassiana, Metarhizium anisopliae, Ear mold, Beauveria bassiana or Penicillium sphaerocephala; The nuclear polyhedrosis virus in the preparation containing nuclear polyhedrosis virus in the active component B is Spodoptera californica nuclear polyhedrosis virus, Spodoptera brassicae nuclear polyhedrosis virus, Spodoptera exigua nuclear polyhedrosis virus, Spodoptera litura nuclear polyhedrosis virus, Helicoverpa armigera nuclear polyhedrosis virus, Tea geometrid nuclear polyhedrosis virus or Spodoptera mulberry nuclear polyhedrosis virus; The cytoplasmic polyhedrosis virus in the preparation containing cytoplasmic polyhedrosis virus is pine caterpillar cytoplasmic polyhedrosis virus. The granular virus in the preparation containing granular virus is Pieris rapae granular virus, Pieris rapae granular virus, Spodoptera exigua granular virus, Pieris rapae granular virus, Plutella xylostella granular virus, Tea leaf roller granular virus, Armyworm granular virus or Rice leaf folder granular virus.

3. The composition according to claim 1 or 2, characterized in that: Component A in the composition is selected from piperic acid derivative compounds; component B is selected from Bacillus thuringiensis, matrine, rotenone, mineral oil, azadirachtin, green anisopliae, Beauveria bassiana, Veratrum rhizome extract, osthole, a preparation containing californica nuclear polyhedrosis virus, and a preparation containing cabbage looper nuclear polyhedrosis virus; the weight ratio of the two active components A and B is 1:60 to 60:

1.

4. The composition according to claim 3, characterized in that: Component A in the composition is selected from piperic acid derivative compounds; component B is selected from matrine, rotenone, mineral oil and azadirachtin; and the weight ratio of the two active components of component A and component B is 1:20 to 60:

1.

5. The composition according to claim 3, characterized in that: Component A in the composition is selected from piperic acid derivative compounds; component B is selected from matrine and rotenone; The weight ratio of the two active components A and B is 1:10 to 10:

1.

6. The composition according to claim 3, characterized in that: Component A in the composition is selected from piperic acid derivative compounds; component B is selected from azadirachtin; The weight ratio of the two active components, component A and component B, is 1:20 to 20:

1.

7. The use of the composition according to claim 1, characterized in that: The composition is used for preparing insecticides and miticides for preventing and controlling pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, and various plant diseases.

Images