Hydrogel antibacterial wound dressing and preparation method thereof

Abstract

The invention belongs to the field of medical dressing preparation, and particularly relates to a hydrogel antibacterial wound dressing and a preparation method thereof. The basic mixture and dipolyglycerol caprate are subjected to a polymerization reaction, a flexible polymer solution is obtained, polyethylene glycol and the flexible polymer solution are subjected to a heating reaction, and the obtained skin-attaching flexible colloid has high water absorption, water retention and elasticity; the antibacterial repair-promoting extract obtained after ultrasonic extraction and concentration is subjected to a synergistic effect with protamine and sodium sulfite, and the obtained stable antibacterial repair-promoting agent has an antibacterial effect and accelerates wound repair; the elastic collagen peptide solution and the skin-fitting flexible nanoparticles are mixed and then subjected to a cross-linking reaction by forming covalent bonds, hydrogel dressing is obtained, and the moisture absorption mucus mixture is evenly sprayed to the surface of the hydrogel dressing mixture. The prepared hydrogel antibacterial wound dressing is high in flexibility, not prone to breakage, high in antibacterial effect and water absorption, comfortable in skin feeling after being used for a long time and free of irritation.

Description

Technical Field

[0001] The invention belongs to the field of medical dressing preparation, and specifically refers to a hydrogel antibacterial wound dressing and a preparation method thereof. Background Art

[0002] Medical hydrogel dressing is a new type of biomedical material, mainly used for wound care. It can absorb and retain water molecules. The amount of water absorbed is closely related to the degree of cross-linking. Hydrogel dressings are widely used in the medical field, especially in drug delivery systems, burn recovery, and postoperative recovery in ophthalmology. The main feature of medical hydrogel dressings is that they can maintain the moist state of the local microenvironment of the wound and promote wound healing. Compared with the traditional "dry healing" method, the cell regeneration ability and migration speed in the wet healing environment are faster, and the recovery speed is faster than in a dry environment. Hydrogel dressings can keep the wound moist, increase cell growth and migration speed, accelerate wound healing, and prevent scab formation.

[0003] Medical hydrogel dressings should be waterproof but able to control water evaporation, maintain the physiological environment of the wound, prevent drying, facilitate the shedding and removal of necrotic tissue, keep the wound clean, reduce the risk of infection, effectively absorb excessive exudate, facilitate drainage and deodorization, facilitate cell migration and the formation of granulation tissue, be easy to remove without damaging the granulation tissue, do not cause pain, be comfortable and prevent abrasions, protect the wound from microbial invasion, and play a barrier protection role. Medical hydrogel dressings, with their unique moist healing properties and biocompatibility, play an increasingly important role in modern wound care and have broad market prospects.

[0004] At present, the existing hydrogel dressing preparation technology has the following problems: first, the existing hydrogel dressing has poor toughness, is easy to break, and has poor mechanical properties; second, the existing hydrogel dressing does not have antibacterial properties, has poor antibacterial ability, has weak adhesion properties, and cannot fit the skin, which limits its clinical use; third, the existing hydrogel dressing cannot be used for wounds with more exudate, has poor water absorption capacity, and feels uncomfortable to the skin after long-term use. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a hydrogel antibacterial wound dressing and a preparation method thereof. In order to solve the problems of poor toughness, easy breakage, poor antibacterial effect and water absorption, and uncomfortable skin feeling after long-term use of the existing hydrogel dressings, the present invention uses a mixture of skin-friendly flexible nanoparticles, antibacterial and repair-promoting microparticles and hygroscopic mucus to prepare a hydrogel antibacterial wound dressing, which has strong toughness, is not easy to break, has strong antibacterial effect and water absorption, and feels comfortable to the skin after long-term use.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention provides a hydrogel antibacterial wound dressing, and the raw materials for preparing the hydrogel antibacterial wound dressing specifically include the following components in parts by weight: 30-50 parts of skin-adhesive flexible nanoparticles, 12-21 parts of antibacterial and repair-promoting microparticles, 18-24 parts of porcine blood vessels, 5-8 parts of elastase, 2-3 parts of transglutaminase, 8-10 parts of cactus mucus, and 4-5 parts of costus lactone.

[0007] The raw materials for preparing the skin-adhering flexible nanoparticles include the following components in parts by weight: 13-17 parts of peach gum, 16-20 parts of glucosamine, 18-24 parts of diglycerol caprate, 3-4 parts of 4-dimethylaminopyridine, and 18-23 parts of polyethylene glycol; The raw materials for preparing the antibacterial and repair-promoting microparticles include the following components in parts by weight: 9-11 parts of juniper, 8-12 parts of red spruce, 9-13 parts of mugwort, 7-11 parts of protamine, 2-4 parts of sodium sulfite, and 14-23 parts of octenyl succinate starch.

[0008] Preferably, the method for preparing the skin-adherent flexible nanoparticles comprises the following steps: S1. Put peach gum into a powdering machine with a power of 1.2-1.5 kW, the powdering speed is 25000 r / min, the powdering time is 8-12 min, the powdering temperature is 25-30°C, and the powdered peach gum and glucosamine are put into a stirring tank with a power of 1.2-1.4 kW, the stirring temperature is 20-26°C, the stirring speed is 120-140 r / min, the stirring time is 10-13 min, and the basic mixture is obtained by stirring and mixing; S2, placing the basic mixture prepared in S1, diglycerol caprate, ultrapure water, and 4-dimethylaminopyridine into a reactor with a power of 2.2-2.6 kW, reacting at a temperature of 160° C. for 7-8 h and a reaction speed of 50-80 r / min to perform a polymerization reaction to obtain a flexible polymer solution; S3, putting polyethylene glycol and the flexible polymer solution prepared in S2 into a vacuum reactor with a power of 6-7 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 120-140°C, a reaction time of 1.5-3.5 h, a reaction speed of 60-90 r / min, and obtaining a skin-adherent flexible colloid after the reaction; S4. Place the skin-adhering flexible colloid prepared by S4 into a vacuum freeze-nano spray dryer with a power of 2.3-2.8 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5-3 h, and perform vacuum freeze-nano spray drying to obtain skin-adhering flexible nanoparticles.

[0009] Furthermore, in step S2, the volume ratio of the ultrapure water to dipolyglycerol caprate is 1.2-1.4:1.

[0010] Preferably, the method for preparing the antibacterial and repair-promoting microparticles comprises the following steps: L1. Put juniper, red spruce and mugwort into a pulverizer with a power of 1.5-1.8 kW, the pulverizing temperature is 23-28°C, the pulverizing time is 15-20 min, the pulverizing speed is 3500-4500 r / min, and after pulverizing, put them into an ultrasonic extractor with a power of 1.3-1.6 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40-45°C, the extraction time is 10-15 min, and after extraction, put them into a traditional Chinese medicine concentrator with a power of 2.5-3.5 kW, the concentration temperature is 40-60°C, the concentration time is 35-45 min, and the concentration speed is 10-20 r / min. After concentration, an antibacterial and repair-promoting extract is obtained; L2, put protamine, sodium sulfite and ultrapure water into a stirring tank with a power of 1.2-1.4 kW, the stirring temperature is 28-32°C, the stirring speed is 100-150 r / min, the stirring time is 12-16 min, after stirring and dissolving, add the antibacterial and pro-repair extract prepared in L1, the stirring temperature is 23-28°C, the stirring speed is 120-140 r / min, the stirring time is 10-16 min, and after stirring, a stable antibacterial and pro-repair agent is obtained; L3, put octenyl succinate starch ester and ultrapure water into a stirring tank with a power of 1.2-1.4 kW, the stirring temperature is 35°C, the stirring speed is 100-150 r / min, the stirring time is 18-22 min, and after stirring and dissolving, a wall material solution is obtained; L4. Put the stable antibacterial and repair-promoting agent prepared by L2 into the wall material solution prepared by L3, and put it into an ultrasonic agitator with a power of 1.8-2.5 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25-30°C, and a stirring time of 10-15 min. After uniform dispersion, put it into a spray dryer with a power of 3.5-3.8 kW, a spray drying temperature of 43-48°C, a spray drying time of 0.8-1.5 s, and a spray drying particle size of 25 mm to obtain antibacterial and repair-promoting microparticles.

[0011] Furthermore, in step L1, the weight ratio of the 70% ethanol solution to juniper is 0.7-0.9:1; in step L2, the mass fraction of the protamine in ultrapure water is 33-37%; in step L3, the mass fraction of the octenyl succinate starch in ultrapure water is 12-15%.

[0012] The present invention also provides a method for preparing a hydrogel antibacterial wound dressing, which specifically comprises the following steps: Step 1: Put the pig blood vessels into a grinder with a power of 1.2-1.5 kW, the grinding temperature is 22-28°C, the grinding time is 14-18 min, the grinding speed is 22000 r / min, and after the grinding, put them into an enzymolysis tank with a power of 1.5-1.8 kW with elastase and ultrapure water, the enzymolysis temperature is 25°C, the enzymolysis time is 6-7.5 h, the enzymolysis speed is 110-140 r / min, and the elastic collagen peptide solution is obtained after enzymolysis; Step 2: Place the skin-adhering flexible nanoparticles, transglutaminase and the elastic collagen peptide solution prepared in step 1 into a vacuum reactor with a power of 6-7 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 50-70°C, a reaction time of 2.5-3.5 h, and a reaction speed of 200-300 r / min to perform a cross-linking reaction to obtain a hydrogel dressing; Step 3: Place the hydrogel dressing prepared in step 2 and the antibacterial and repair-promoting microparticles into a stirring tank with a power of 1.2-1.4 kW, stir at a temperature of 23-28° C., stir at a speed of 100-150 r / min, and stir for 14-22 min, mix well, and obtain a hydrogel dressing mixture; Step 4: Place the cactus mucus and costus lactone into a stirring tank with a power of 1.2-1.4 kW, with a stirring temperature of 20-26° C., a stirring speed of 130-160 r / min, and a stirring time of 7-12 min to obtain a hygroscopic mucus mixture; Step 5: Use a sprayer with a power of 1.6-2.3 kW, spray the hygroscopic mucus mixture prepared in step 4 evenly on the surface of the hydrogel dressing mixture prepared in step 3, spray for 13-18 min, and spray at a temperature of 25-30°C. After spraying, place it under ventilation conditions at 23-28°C for 3-4 hours to obtain a hydrogel antibacterial wound dressing.

[0013] Preferably, in step 1, the weight ratio of the ultrapure water to the porcine blood vessel is 1.3-1.5:1.

[0014] The beneficial effects achieved by the present invention are as follows: The invention uses peach gum to be powdered and then mixed with glucosamine. Under the catalytic action of 4-dimethylaminopyridine, the sugar molecules contained in the obtained basic mixture and dipolyglycerol caprate can act as free radicals to initiate the polymerization of dipolyglycerol caprate monomers, and form new dipolyglycerol caprate chain segments on the sugar molecules. The dipolyglycerol caprate has biocompatibility and high elasticity. The peach gum and glucosamine have strong water absorption. The prepared flexible polymer has strong water absorption, toughness and improved elasticity. Polyethylene glycol and the flexible polymer solution are heated to react. The polyethylene glycol molecules contain a large amount of The ether bonds and hydroxyl groups can form hydrogen bonds with water molecules. The hydrophilicity of the polyethylene glycol molecules can reduce protein adsorption and cell adhesion on the surface of the polymer solution components, further improving its stability and biocompatibility in the body. The polyethylene glycol promotes the formation of a network structure by forming hydrogen bonds, thereby obtaining a skin-applicable flexible colloid with high water absorption and water retention. The skin-applicable flexible colloid is freeze-nano spray-dried, and the prepared skin-applicable flexible nanoparticles have strong stability during the preparation process; juniper, red spruce, and mugwort are crushed and extracted and concentrated with a 70% ethanol solution to prepare an anti- The bacteria-promoting repair extract contains antibacterial compounds that promote wound repair, such as hinokitiol, 4-methyl-1-(1-methylethyl) cyclohexene, terpinene, β-amyrin acetate, and the like. The antibacterial-promoting repair extract also contains polyphenolic compounds such as stigma biflavonoids, myricetin, and daidzein. The protamine and sodium sulfite synergistically act as antibacterial-promoting repair extracts. The obtained stable antibacterial-promoting repair agent will not deteriorate, has an anti-inflammatory effect, and accelerates wound repair. The antibacterial-promoting repair agent is dispersed in a wall material solution, and the prepared antibacterial-promoting repair microparticles are effective in antibacterial and accelerating wound repair. Pig blood vessels are rich in elastic collagen. The elastic collagen peptide solution obtained after enzymatic hydrolysis of pig blood vessels is mixed with skin-adapting flexible nanoparticles under the catalysis of transglutaminase to undergo cross-linking reaction by forming covalent bonds to obtain a hydrogel dressing. Cactus mucus and costus lactone have water absorption, antioxidant, anti-inflammatory, and anti-allergic effects, and are sticky. The hygroscopic mucus mixture is evenly sprayed on the surface of the hydrogel dressing mixture. The prepared hydrogel antibacterial wound dressing has strong toughness, is not easy to break, has strong antibacterial effect and water absorption, and is comfortable and non-irritating to the skin when used for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 This is a scanning electron microscope image of the internal structure of the hydrogel antibacterial wound dressing of the present invention; Figure 2 This is a graph showing the tensile strength results of the hydrogel dressing described in Experimental Example 1 of the present invention; Figure 3 This is a graph showing the bacterial inhibition rate results described in Experimental Example 2 of the present invention; Figure 4 This is a graph showing the water absorption rate of the hydrogel dressing described in Experimental Example 3 of the present invention. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0018] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0019] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0020] Example 1: This example provides a hydrogel antibacterial wound dressing and a preparation method thereof. The raw materials for preparing the hydrogel antibacterial wound dressing specifically include the following components in parts by weight: 30 parts of skin-adhesive flexible nanoparticles, 12 parts of antibacterial and repair-promoting microparticles, 18 parts of porcine blood vessels, 5 parts of elastase, 2 parts of transglutaminase, 8 parts of cactus mucus, and 4 parts of costus lactone.

[0021] The raw materials for preparing the skin-adhering flexible nanoparticles include the following components in parts by weight: 13 parts of peach gum, 16 parts of glucosamine, 18 parts of diglycerol caprate, 3 parts of 4-dimethylaminopyridine, and 18 parts of polyethylene glycol; The raw materials for preparing the antibacterial and repair-promoting microparticles include the following components in parts by weight: 9 parts of juniper, 8 parts of red spruce, 9 parts of mugwort, 7 parts of protamine, 2 parts of sodium sulfite, and 14 parts of octenyl succinate starch.

[0022] The method for preparing the skin-adherent flexible nanoparticles specifically comprises the following steps: S1. Put peach gum into a powdering machine with a power of 1.2 kW, the powdering speed is 25000 r / min, the powdering time is 8 min, the powdering temperature is 25°C, and the powdered peach gum and glucosamine are put into a stirring tank with a power of 1.2 kW, the stirring temperature is 20°C, the stirring speed is 120 r / min, the stirring time is 10 min, and the basic mixture is obtained by stirring and mixing; S2, placing the basic mixture prepared in S1, diglycerol caprate, ultrapure water, and 4-dimethylaminopyridine into a reactor with a power of 2.2 kW, the reaction temperature is 160°C, the reaction time is 7 h, the reaction speed is 50 r / min, and a polymerization reaction is carried out to obtain a flexible polymer solution; S3, putting polyethylene glycol and the flexible polymer solution prepared in S2 into a vacuum reactor with a power of 6 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 120°C, a reaction time of 1.5 h, and a reaction speed of 60 r / min, and obtaining a skin-adherent flexible colloid after the reaction; S4. Place the skin-adhering flexible colloid prepared by S4 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h, and perform vacuum freeze-nano spray drying to obtain skin-adhering flexible nanoparticles.

[0023] In step S2, the volume ratio of the ultrapure water to dipolyglycerol caprate is 1.2:1.

[0024] The method for preparing the antibacterial and repair-promoting microparticles specifically comprises the following steps: L1. Put juniper, red spruce and mugwort into a 1.5 kW grinder, the grinding temperature is 23°C, the grinding time is 15 min, the grinding speed is 3500 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.3 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40°C, the extraction time is 10 min, and after extraction, put them into a traditional Chinese medicine concentrator with a power of 2.5 kW, the concentration temperature is 40°C, the concentration time is 35 min, and the concentration speed is 10 r / min. After concentration, the antibacterial and repair-promoting extract is obtained; L2, put protamine, sodium sulfite and ultrapure water into a stirring tank with a power of 1.2 kW, the stirring temperature is 28°C, the stirring speed is 100 r / min, the stirring time is 12 min, after stirring and dissolving, add the antibacterial and repair-promoting extract prepared in L1, the stirring temperature is 23°C, the stirring speed is 120 r / min, the stirring time is 10 min, and after stirring, a stable antibacterial and repair-promoting agent is obtained; L3, put octenyl succinate starch ester and ultrapure water into a stirring tank with a power of 1.2 kW, the stirring temperature is 35°C, the stirring speed is 100 r / min, the stirring time is 18 min, and after stirring and dissolving, a wall material solution is obtained; L4. Put the stable antibacterial and repair-promoting agent prepared by L2 into the wall material solution prepared by L3, and put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min. After uniform dispersion, put it into a spray dryer with a power of 3.5 kW, a spray drying temperature of 43°C, a spray drying time of 0.8 s, and a spray drying particle size of 25 mm to obtain antibacterial and repair-promoting microparticles.

[0025] In step L1, the weight ratio of the 70% ethanol solution to juniper is 0.7:1; in step L2, the mass fraction of protamine in ultrapure water is 33%; in step L3, the mass fraction of octenyl succinate starch in ultrapure water is 12%.

[0026] This embodiment also provides a method for preparing a hydrogel antibacterial wound dressing, which specifically comprises the following steps: Step 1: Put the pig blood vessels into a pulverizer with a power of 1.2 kW, the pulverizing temperature is 22°C, the pulverizing time is 14 minutes, the pulverizing speed is 22000 r / min, and after pulverizing, put them into an enzymatic hydrolysis tank with a power of 1.5 kW with elastase and ultrapure water, the enzymatic hydrolysis temperature is 25°C, the enzymatic hydrolysis time is 6 hours, the enzymatic hydrolysis speed is 110 r / min, and the elastic collagen peptide solution is obtained after enzymatic hydrolysis; Step 2: Place the skin-adhering flexible nanoparticles, transglutaminase and the elastic collagen peptide solution prepared in step 1 into a vacuum reactor with a power of 6 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 50°C, a reaction time of 2.5 h, and a reaction speed of 200 r / min to perform a cross-linking reaction to obtain a hydrogel dressing; Step 3: Place the hydrogel dressing prepared in step 2 and the antibacterial and repair-promoting microparticles into a stirring tank with a power of 1.2 kW, stir at a temperature of 23° C., stir at a speed of 100 r / min, stir for 14 min, mix well, and obtain a hydrogel dressing mixture; Step 4: Place the cactus mucus and costus lactone into a stirring tank with a power of 1.2 kW, with a stirring temperature of 20° C., a stirring speed of 130 r / min, and a stirring time of 7 min to obtain a hygroscopic mucus mixture; Step 5: Use a 1.6 kW sprayer to evenly spray the hygroscopic mucus mixture prepared in step 4 on the surface of the hydrogel dressing mixture prepared in step 3 for 13 min at a spraying temperature of 25°C. After spraying, place it at 23°C under ventilation conditions for 3 h to obtain a hydrogel antibacterial wound dressing.

[0027] In step 1, the weight ratio of the ultrapure water to the pig blood vessel is 1.3:1.

[0028] Example 2: This example provides a hydrogel antibacterial wound dressing and a preparation method thereof. The raw materials for preparing the hydrogel antibacterial wound dressing specifically include the following components in parts by weight: 40 parts of skin-friendly flexible nanoparticles, 18 parts of antibacterial and repair-promoting microparticles, 21 parts of porcine blood vessels, 6 parts of elastase, 2.5 parts of transglutaminase, 9 parts of cactus mucus, and 4.5 parts of costus lactone.

[0029] The raw materials for preparing the skin-adhering flexible nanoparticles include the following components in parts by weight: 15 parts of peach gum, 18 parts of glucosamine, 22 parts of diglycerol caprate, 3.5 parts of 4-dimethylaminopyridine, and 21 parts of polyethylene glycol; The raw materials for preparing the antibacterial and repair-promoting microparticles include the following components in parts by weight: 10 parts of juniper, 10 parts of red spruce, 11 parts of mugwort, 9 parts of protamine, 3 parts of sodium sulfite, and 17 parts of octenyl succinate starch.

[0030] The method for preparing the skin-adherent flexible nanoparticles specifically comprises the following steps: S1. Put peach gum into a powdering machine with a power of 1.3 kW, the powdering speed is 25000 r / min, the powdering time is 10 min, the powdering temperature is 28°C, and the powdered peach gum and glucosamine are put into a stirring tank with a power of 1.3 kW, the stirring temperature is 23°C, the stirring speed is 130 r / min, the stirring time is 12 min, and the basic mixture is obtained by stirring and mixing; S2, placing the basic mixture prepared in S1, diglycerol caprate, ultrapure water, and 4-dimethylaminopyridine into a reactor with a power of 2.4 kW, the reaction temperature is 160°C, the reaction time is 7.5 h, the reaction speed is 60 r / min, and a polymerization reaction is carried out to obtain a flexible polymer solution; S3, putting polyethylene glycol and the flexible polymer solution prepared in S2 into a vacuum reactor with a power of 6.5 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 130°C, a reaction time of 2.5 h, and a reaction speed of 80 r / min, and obtaining a skin-adherent flexible colloid after the reaction; S4. Place the skin-adhering flexible colloid prepared by S4 into a vacuum freeze-nano spray dryer with a power of 2.6 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.8 h, and perform vacuum freeze-nano spray drying to obtain skin-adhering flexible nanoparticles.

[0031] In step S2, the volume ratio of the ultrapure water to dipolyglycerol caprate is 1.3:1.

[0032] The method for preparing the antibacterial and repair-promoting microparticles specifically comprises the following steps: L1. Put juniper, red spruce and mugwort into a 1.6 kW grinder, the grinding temperature is 25°C, the grinding time is 18 min, the grinding speed is 4000 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.5 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 43°C, the extraction time is 13 min, and after extraction, put them into a traditional Chinese medicine concentrator with a power of 3 kW, the concentration temperature is 50°C, the concentration time is 40 min, and the concentration speed is 20 r / min. After concentration, the antibacterial and repair-promoting extract is obtained; L2, put protamine, sodium sulfite and ultrapure water into a stirring tank with a power of 1.3 kW, the stirring temperature is 30°C, the stirring speed is 130 r / min, the stirring time is 14 min, after stirring and dissolving, add the antibacterial and repair-promoting extract prepared in L1, the stirring temperature is 26°C, the stirring speed is 130 r / min, the stirring time is 13 min, and after stirring, a stable antibacterial and repair-promoting agent is obtained; L3, put octenyl succinate starch ester and ultrapure water into a stirring tank with a power of 1.3 kW, the stirring temperature is 35°C, the stirring speed is 130 r / min, the stirring time is 20 min, and after stirring and dissolving, a wall material solution is obtained; L4. Put the stable antibacterial and repair-promoting agent prepared by L2 into the wall material solution prepared by L3, and put it into an ultrasonic agitator with a power of 2.2 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 28°C, and a stirring time of 13 min. After uniform dispersion, put it into a spray dryer with a power of 3.6 kW, a spray drying temperature of 46°C, a spray drying time of 1.2 s, and a spray drying particle size of 25 mm to obtain antibacterial and repair-promoting microparticles.

[0033] In step L1, the weight ratio of the 70% ethanol solution to juniper is 0.8:1; in step L2, the mass fraction of protamine in ultrapure water is 35%; in step L3, the mass fraction of octenyl succinate starch in ultrapure water is 13%.

[0034] This embodiment also provides a method for preparing a hydrogel antibacterial wound dressing, which specifically comprises the following steps: Step 1: Put the pig blood vessels into a pulverizer with a power of 1.3 kW, the pulverizing temperature is 26°C, the pulverizing time is 16 minutes, the pulverizing speed is 22000 r / min, and after pulverization, put them into an enzymatic hydrolysis tank with a power of 1.7 kW with elastase and ultrapure water, the enzymatic hydrolysis temperature is 25°C, the enzymatic hydrolysis time is 6.5 hours, the enzymatic hydrolysis speed is 130 r / min, and the elastic collagen peptide solution is obtained after enzymatic hydrolysis; Step 2: Place the skin-adhering flexible nanoparticles, transglutaminase and the elastic collagen peptide solution prepared in step 1 into a vacuum reactor with a power of 6.5 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 60°C, a reaction time of 3 hours, and a reaction speed of 200 r / min to perform a cross-linking reaction to obtain a hydrogel dressing; Step 3: Place the hydrogel dressing prepared in step 2 and the antibacterial and repair-promoting microparticles into a stirring tank with a power of 1.3 kW, stir at a temperature of 26° C., stir at a speed of 130 r / min, stir for 18 min, mix well, and obtain a hydrogel dressing mixture; Step 4: Place the cactus mucus and costus lactone into a stirring tank with a power of 1.3 kW, with a stirring temperature of 23° C., a stirring speed of 140 r / min, and a stirring time of 10 min to obtain a hygroscopic mucus mixture; Step 5: Use a 2.1 kW sprayer to evenly spray the hygroscopic mucus mixture prepared in step 4 on the surface of the hydrogel dressing mixture prepared in step 3 for 16 min at a spraying temperature of 28°C. After spraying, place it at 25°C for 3.5 hours under ventilation conditions to obtain a hydrogel antibacterial wound dressing.

[0035] In step 1, the weight ratio of the ultrapure water to the pig blood vessel is 1.4:1.

[0036] Example 3: This example provides a hydrogel antibacterial wound dressing and a preparation method thereof. The raw materials for preparing the hydrogel antibacterial wound dressing specifically include the following components in parts by weight: 50 parts of skin-adhesive flexible nanoparticles, 21 parts of antibacterial and repair-promoting microparticles, 24 parts of porcine blood vessels, 8 parts of elastase, 3 parts of transglutaminase, 10 parts of cactus mucus, and 5 parts of costus lactone.

[0037] The raw materials for preparing the skin-adhering flexible nanoparticles include the following components in parts by weight: 17 parts of peach gum, 20 parts of glucosamine, 24 parts of diglycerol caprate, 4 parts of 4-dimethylaminopyridine, and 23 parts of polyethylene glycol; The raw materials for preparing the antibacterial and repair-promoting microparticles include the following components in parts by weight: 11 parts of juniper, 12 parts of red spruce, 13 parts of mugwort, 11 parts of protamine, 4 parts of sodium sulfite, and 23 parts of octenyl succinate starch.

[0038] The method for preparing the skin-adherent flexible nanoparticles specifically comprises the following steps: S1. Put peach gum into a powdering machine with a power of 1.5 kW, the powdering speed is 25000 r / min, the powdering time is 12 min, the powdering temperature is 30°C, and the powdered peach gum and glucosamine are put into a stirring tank with a power of 1.4 kW, the stirring temperature is 26°C, the stirring speed is 140 r / min, the stirring time is 13 min, and the basic mixture is obtained by stirring and mixing; S2, placing the basic mixture prepared in S1, diglycerol caprate, ultrapure water, and 4-dimethylaminopyridine into a reactor with a power of 2.6 kW, the reaction temperature is 160°C, the reaction time is 8 h, the reaction speed is 80 r / min, and a polymerization reaction is carried out to obtain a flexible polymer solution; S3, putting polyethylene glycol and the flexible polymer solution prepared in S2 into a vacuum reactor with a power of 7 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 140°C, a reaction time of 3.5 h, and a reaction speed of 90 r / min, and obtaining a skin-adherent flexible colloid after the reaction; S4. Place the skin-adhering flexible colloid prepared by S4 into a vacuum freeze-nano spray dryer with a power of 2.8 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 3 h, and perform vacuum freeze-nano spray drying to obtain skin-adhering flexible nanoparticles.

[0039] In step S2, the volume ratio of the ultrapure water to dipolyglycerol caprate is 1.4:1.

[0040] The method for preparing the antibacterial and repair-promoting microparticles specifically comprises the following steps: L1. Put juniper, red spruce and mugwort into a 1.8 kW grinder, the grinding temperature is 28°C, the grinding time is 20 min, the grinding speed is 4500 r / min, and after grinding, put them into an ultrasonic extractor with a power of 1.6 kW with a 70% ethanol solution, the extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 45°C, the extraction time is 15 min, and after extraction, put them into a traditional Chinese medicine concentrator with a power of 3.5 kW, the concentration temperature is 60°C, the concentration time is 45 min, the concentration speed is 20 r / min, and the antibacterial and repairing extract is obtained after concentration; L2, put protamine, sodium sulfite and ultrapure water into a stirring tank with a power of 1.4 kW, the stirring temperature is 32°C, the stirring speed is 150 r / min, the stirring time is 16 min, after stirring and dissolving, add the antibacterial and repair-promoting extract prepared in L1, the stirring temperature is 28°C, the stirring speed is 140 r / min, the stirring time is 16 min, and after stirring, a stable antibacterial and repair-promoting agent is obtained; L3, put octenyl succinate starch ester and ultrapure water into a stirring tank with a power of 1.4 kW, the stirring temperature is 35°C, the stirring speed is 150 r / min, the stirring time is 22 min, and after stirring and dissolving, a wall material solution is obtained; L4. Put the stable antibacterial and repair-promoting agent prepared by L2 into the wall material solution prepared by L3, and put it into an ultrasonic agitator with a power of 2.5 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 30°C, and a stirring time of 15 min. After uniform dispersion, put it into a spray dryer with a power of 3.8 kW, a spray drying temperature of 48°C, a spray drying time of 1.5 s, and a spray drying particle size of 25 mm to obtain antibacterial and repair-promoting microparticles.

[0041] In step L1, the weight ratio of the 70% ethanol solution to juniper is 0.9:1; in step L2, the mass fraction of protamine in ultrapure water is 37%; in step L3, the mass fraction of octenyl succinate starch in ultrapure water is 15%.

[0042] This embodiment also provides a method for preparing a hydrogel antibacterial wound dressing, which specifically comprises the following steps: Step 1: Put the pig blood vessels into a pulverizer with a power of 1.5 kW, the pulverizing temperature is 28°C, the pulverizing time is 18 minutes, the pulverizing speed is 22000 r / min, and after pulverization, put them into an enzymatic hydrolysis tank with a power of 1.8 kW with elastase and ultrapure water, the enzymatic hydrolysis temperature is 25°C, the enzymatic hydrolysis time is 7.5 hours, the enzymatic hydrolysis speed is 140 r / min, and the elastic collagen peptide solution is obtained after enzymatic hydrolysis; Step 2: Place the skin-adhering flexible nanoparticles, transglutaminase and the elastic collagen peptide solution prepared in step 1 into a vacuum reactor with a power of 7 kW, a vacuum degree of -0.09 MPa, a reaction temperature of 70°C, a reaction time of 3.5 hours, and a reaction speed of 300 r / min to perform a cross-linking reaction to obtain a hydrogel dressing; Step 3: Place the hydrogel dressing prepared in step 2 and the antibacterial and repair-promoting microparticles into a stirring tank with a power of 1.4 kW, stir at a temperature of 28° C., stir at a speed of 150 r / min, stir for 22 min, mix well, and obtain a hydrogel dressing mixture; Step 4: Place the cactus mucus and costus lactone into a stirring tank with a power of 1.4 kW, with a stirring temperature of 26° C., a stirring speed of 160 r / min, and a stirring time of 12 min to obtain a hygroscopic mucus mixture; Step 5: Use a 2.3 kW sprayer to evenly spray the hygroscopic mucus mixture prepared in step 4 on the surface of the hydrogel dressing mixture prepared in step 3 for 18 min at a spraying temperature of 30°C. After spraying, place it at 28°C for 4 hours under ventilation to obtain a hydrogel antibacterial wound dressing.

[0043] In step 1, the weight ratio of the ultrapure water to the pig blood vessel is 1.5:1.

[0044] Comparative Example 1: This comparative example provides a hydrogel wound dressing and a preparation method thereof, which is different from Example 1 only in that diglycerol caprate is not added to the prepared skin-adhering flexible nanoparticles, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0045] Comparative Example 2: This comparative example provides a hydrogel wound dressing and a preparation method thereof, which is different from Example 1 only in that no antibacterial and pro-repair extract is added to the prepared antibacterial and pro-repair microparticles, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0046] Experimental Example 1: Tensile strength measurement test.

[0047] The steps for testing the tensile strength of the hydrogel antibacterial wound dressing prepared in Examples 1-3 of the present invention before and after recovery are as follows: (1) Weigh 8 g of the hydrogel antibacterial wound dressings prepared in Examples 1-3 as the experimental group, weigh 8 g of the hydrogel wound dressings prepared in Comparative Examples 1-2 as the comparative group, and weigh 8 g of commercially available hydrogel wound dressings (purchased from Wuhan Huawei Medical Device Co., Ltd.) as the CK control group. Weigh 8 parallels for each group; (2) The water-absorbent materials of the experimental group, comparative group and control group were molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". Specifically, a 2 kN fixture was used, the inlet force was 0.1 N, the tensile rate was 30 mm / min, and 8 specimens were stretched in each group to obtain the average value. The tensile strength of the hydrogel antibacterial wound dressing of the experimental group, the hydrogel wound dressing of the comparative group and the commercially available hydrogel wound dressing of the control group before recovery was obtained; (3) soaking the experimental group hydrogel antibacterial wound dressing, the comparative group hydrogel wound dressing and the control group commercially available hydrogel wound dressing, which have been subjected to the tensile strength test in step (2), in a sufficient amount of ultrapure water until the volume no longer changes, and then placing them in a drying oven for drying until the experimental group hydrogel antibacterial wound dressing, the comparative group hydrogel wound dressing and the control group commercially available hydrogel wound dressing recover to their original weight, thereby obtaining the recovered experimental group hydrogel antibacterial wound dressing, the comparative group hydrogel wound dressing and the control group commercially available hydrogel wound dressing; (4) The recovered hydrogel antibacterial wound dressings in the experimental group, the hydrogel wound dressings in the comparative group and the commercially available hydrogel wound dressings in the control group were molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". Specifically, a 2 kN clamp was selected, the inlet force was 0.1 N, the tensile rate was 30 mm / min, and 8 specimens were stretched in each group to obtain the average value. The tensile strength of the recovered hydrogel antibacterial wound dressings in the experimental group, the hydrogel wound dressings in the comparative group and the commercially available hydrogel wound dressings in the control group was obtained.

[0048] Result analysis: Figure 2This is a tensile strength result diagram of the hydrogel dressing described in Experimental Example 1 of the present invention. As shown in the figure, the hydrogel antibacterial wound dressings prepared in Examples 1-3 are molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths are 8.1 MPa, 8.6 MPa, and 9.4 MPa. After recovery, the hydrogel antibacterial wound dressings prepared in Examples 1-3 are molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths are 8.0 MPa, 8.4 MPa, and 9.0 MPa. The hydrogel wound dressings prepared in Comparative Example 1 are molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T The hydrogel wound dressing prepared in the comparative example 1 group after recovery was molded into a dumbbell-shaped tensile specimen specimen, and the tensile strength was 4.1 MPa. The hydrogel wound dressing prepared in the comparative example 2 group was molded into a dumbbell-shaped tensile specimen specimen, and the tensile strength was 8.1 MPa. The hydrogel wound dressing prepared in the comparative example 1 group after recovery was molded into a dumbbell-shaped tensile specimen specimen, and the tensile strength was 7.9 MPa according to the method described in the standard GB / T 1040.3-2006. The commercially available hydrogel wound dressing in the control group was molded into a dumbbell-shaped tensile specimen specimen, and the tensile strength was 2.7 according to the method described in the standard GB / T 1040.3-2006. MPa, and after recovery, the commercially available hydrogel wound dressing in the control group was molded into dumbbell-shaped tensile test specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strength was 1.8 MPa, indicating that the hydrogel antibacterial wound dressing prepared by the present invention has strong toughness, is not easy to break, and has excellent mechanical properties.

[0049] Experimental Example 2: Antibacterial ability determination test.

[0050] The antibacterial ability test steps of the hydrogel antibacterial wound dressing prepared in Examples 1-3 of the present invention are as follows: (1) Take out Staphylococcus aureus and Streptococcus from the -80℃ refrigerator as test strains; (2) In a sterile clean bench, take 10 mL of Staphylococcus aureus and Streptococcus liquid respectively and inoculate them into test tubes containing 10 mL of sterile liquid malt extract medium. Take 18 tubes of each and divide them into 6 groups, with 3 tubes in each group. (3) placing the test tube containing the Staphylococcus aureus and Streptococcus culture liquid in step (2) in a constant temperature shaker at 37°C and a rotation speed of 120 r / min, and shaking culture for 24 hours to obtain cultured Staphylococcus aureus and Streptococcus culture liquid; (4) In a sterile clean bench, 2 g of the hydrogel antibacterial wound dressing prepared in Example 1-3 and the hydrogel wound dressing prepared in Comparative Example 1-2 were added to 6 groups of cultured Staphylococcus aureus liquid and 6 groups of Streptococcus liquid, respectively. A CK control group was formed by adding 2 g of commercially available hydrogel wound dressing (purchased from Wuhan Huawei Medical Instrument Co., Ltd.); (5) All the test tubes of the experimental group, comparative group, and CK group in step (4) were placed in a constant temperature shaker at 37°C and 120 r / min for 30 min; (6) Take 35 mL of the liquid in the test tube in step (5) and place it on a sterile solid malt extract agar plate. Use a sterile spreading rod to evenly spread the plate. After spreading, seal the plate with a sealing film. (7) Place the sealed sterile solid malt extract agar plate in step (6) upside down in a constant temperature incubator at 37°C and incubate for 24 h. Observe the number of colonies on the plate and calculate the inhibition rate. Take the average value. Inhibition rate (%) = number of colonies in the control group - number of colonies in the experimental group / number of colonies in the control group × 100%. Record the result.

[0051] Result analysis: Figure 3This is a result graph of the bacterial inhibition rate described in Experimental Example 2 of the present invention. As shown in the figure, the hydrogel antibacterial wound dressings prepared in Examples 1-3 were placed in a Staphylococcus aureus solution, and the inhibition rates of Staphylococcus aureus for oscillation culture were 95.9%, 96.9%, and 98.6%, respectively. The hydrogel antibacterial wound dressings prepared in Examples 1-3 were placed in a Streptococcus solution, and the inhibition rates of Streptococcus for oscillation culture were 97.7%, 98.1%, and 98.9%, respectively. The hydrogel wound dressing prepared in Comparative Example 1 was placed in a Staphylococcus aureus solution, and the inhibition rate of Staphylococcus aureus for oscillation culture was 93.7%. The hydrogel wound dressing prepared in Comparative Example 1 was placed in a Streptococcus solution, and the inhibition rate of Streptococcus for oscillation culture was 93.7%. The inhibition rate of cocci is 95.8%, the hydrogel wound dressing prepared in comparative example 2 is placed in a Staphylococcus aureus liquid, and the inhibition rate of Staphylococcus aureus by oscillation culture is 50.3%, the hydrogel wound dressing prepared in comparative example 2 is placed in a streptococcus liquid, and the inhibition rate of streptococcus by oscillation culture is 52.5%, the commercially available hydrogel wound dressing in the control group is placed in a Staphylococcus aureus liquid, and the inhibition rate of Staphylococcus aureus by oscillation culture is 25.9%, the commercially available hydrogel wound dressing in the control group is placed in a streptococcus liquid, and the inhibition rate of streptococcus by oscillation culture is 27.8%, indicating that the hydrogel antibacterial wound dressing prepared by the present invention has antibacterial properties, effectively inhibits the growth of bacteria, and accelerates wound repair.

[0052] Experimental Example 3: Water absorption and skin irritation test.

[0053] The test steps for measuring water absorption and skin irritation of the hydrogel antibacterial wound dressing prepared in Examples 1-3 of the present invention are as follows: (1) Weigh 8 g of the hydrogel antibacterial wound dressings prepared in Examples 1-3 as the experimental group, weigh 8 g of the hydrogel wound dressings prepared in Comparative Examples 1-2 as the comparative group, and weigh 8 g of commercially available hydrogel wound dressings (purchased from Wuhan Huawei Medical Device Co., Ltd.) as the CK control group. Weigh 8 parallels for each group; (2) The water absorption rate of the hydrogel was tested according to GB / T8939-2008, and then calculated according to the following formula: water absorption rate (g / g) = (mass after saturated water absorption - mass before absorption) / mass before absorption; (3) Sixty healthy white male guinea pigs weighing 700 g were randomly divided into six groups of 10; (2) Conventional hair removal was performed on both sides of the back of white guinea pigs, with a hair removal range of 3 cm × 3 cm. After 24 hours of hair removal, the local skin was observed to be intact and without damage. The experiment used the left and right sides of the same guinea pig for self-comparison. The left side of the back of the guinea pigs in the experimental groups 1-3 was smeared with the hydrogel antibacterial wound dressings prepared in Examples 1-3, the left side of the back of the guinea pigs in the comparative example group was smeared with the hydrogel wound dressings prepared in Comparative Example 1-2, and the left side of the back of the guinea pigs in the control group was smeared with a commercially available hydrogel wound dressing. The guinea pigs in each group were smeared once a day for 10 hours each time. After continuous use for 10 days, the treated area was cleaned with warm water and observed; (3) Observe with the naked eye 24, 48, and 72 hours after cleaning with warm water to see if there is any erythema or edema on the treated area on the left side of the guinea pig's back. Calculate the average score of each group of guinea pigs at each observation time point according to Table 1, and evaluate the intensity of stimulation according to Table 2.

[0054] Table 1. Skin irritation response scoring criteria

[0055] Table 2. Skin irritation intensity evaluation criteria

[0056] Table 3. Skin irritation reaction results

[0057] Result analysis: Figure 1 This is a scanning electron microscope image of the internal structure of the hydrogel antibacterial wound dressing of the present invention. Figure 4 This is a graph showing the water absorption rate of the hydrogel dressing described in Experimental Example 3 of the present invention. Figure 4As shown in Table 3, the hydrogel antibacterial wound dressings prepared in Examples 1-3 were tested for water absorption according to GB / T8939-2008, and the water absorption was 685 g / g, 691 g / g, and 703 g / g, respectively. The hydrogel wound dressing prepared in Comparative Example 1 was tested for water absorption according to GB / T8939-2008, and the water absorption was 425 g / g. The hydrogel wound dressing prepared in Comparative Example 2 was tested for water absorption according to GB / T8939-2008, and the water absorption was 679 g / g. The commercially available hydrogel wound dressing in the control group was tested for water absorption according to GB / T8939-2008, and the water absorption was 382 g / g, the physiological structure of guinea pig skin is similar to that of human skin, the antibacterial hydrogel wound dressings prepared in Example 1-3 and the antibacterial hydrogel wound dressings prepared in Comparative Example 1-2 were applied to the back of guinea pigs for a long time, and no erythema and edema appeared on the back of the white guinea pigs, while the commercially available hydrogel wound dressings in the control group were applied to the back of guinea pigs for a long time, and mild irritation appeared on the back of the white guinea pigs, indicating that the antibacterial hydrogel wound dressing prepared in the present invention has strong water absorption, can be used for wounds with more exudate, and has good skin feel and no irritation after long-term use.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0059] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A hydrogel antibacterial wound dressing, characterized in that: The raw materials for preparing the hydrogel antibacterial wound dressing specifically include the following components in parts by weight: 30-50 parts of skin-friendly flexible nanoparticles, 12-21 parts of antibacterial and repair-promoting microparticles, 18-24 parts of porcine blood vessels, 5-8 parts of elastase, 2-3 parts of transglutaminase, 8-10 parts of cactus mucus, and 4-5 parts of costus lactone; The raw materials for preparing the skin-adhering flexible nanoparticles include the following components in parts by weight: 13-17 parts of peach gum, 16-20 parts of glucosamine, 18-24 parts of diglycerol caprate, 3-4 parts of 4-dimethylaminopyridine, and 18-23 parts of polyethylene glycol; The raw materials for preparing the antibacterial and repair-promoting microparticles include the following components in parts by weight: 9-11 parts of juniper, 8-12 parts of red spruce, 9-13 parts of mugwort, 7-11 parts of protamine, 2-4 parts of sodium sulfite, and 14-23 parts of octenyl succinate starch.

2. The hydrogel antibacterial wound dressing according to claim 1, characterized in that: The method for preparing the skin-adherent flexible nanoparticles specifically comprises the following steps: S1, grinding peach gum into powder, and mixing with glucosamine to obtain a basic mixture; S2, polymerizing the basic mixture prepared in S1 with dipolyglycerol caprate, ultrapure water, and 4-dimethylaminopyridine to obtain a flexible polymer solution; S3, mixing polyethylene glycol with the flexible polymer solution prepared in S2 to obtain a skin-adherent flexible colloid; S4. The skin-adhering flexible colloid prepared in S4 is subjected to vacuum freeze nano-spray drying to obtain skin-adhering flexible nanoparticles.

3. The hydrogel antibacterial wound dressing according to claim 2, characterized in that: In step S2, the volume ratio of the ultrapure water to dipolyglycerol caprate is 1.2-1.4:

1.

4. The hydrogel antibacterial wound dressing according to claim 3, characterized in that: The method for preparing the antibacterial and repair-promoting microparticles specifically comprises the following steps: L1. After crushing juniper, red spruce and mugwort, ultrasonic extraction and concentration were performed with 70% ethanol solution to obtain an antibacterial and repair-promoting extract; L2, dissolving protamine and sodium sulfite in ultrapure water, adding the antibacterial and repair-promoting extract prepared in L1, stirring and mixing to obtain a stable antibacterial and repair-promoting agent; L3, fully dissolving octenyl succinate starch ester in ultrapure water to obtain a wall material solution; L4, uniformly dispersing the stable antibacterial and repair-promoting agent prepared in L2 in the wall material solution prepared in L3, and spray-drying the solution to obtain antibacterial and repair-promoting microparticles.

5. The hydrogel antibacterial wound dressing according to claim 4, characterized in that: In step L1, the weight ratio of the 70% ethanol solution to juniper is 0.7-0.9:

1.

6. The hydrogel antibacterial wound dressing according to claim 5, characterized in that: In step L2, the mass fraction of protamine in ultrapure water is 33-37%.

7. The hydrogel antibacterial wound dressing according to claim 6, characterized in that: In step L3, the mass fraction of the octenyl succinate starch ester in ultrapure water is 12-15%.

8. A method for preparing the hydrogel antibacterial wound dressing according to any one of claims 1 to 7, characterized in that: The specific steps include: Step 1: crush the pig blood vessels, mix them with elastase and ultrapure water, and then enzymatically hydrolyze them to obtain an elastic collagen peptide solution; Step 2: Mix the skin-adhering flexible nanoparticles and transglutaminase with the elastic collagen peptide solution prepared in step 1, and perform a cross-linking reaction to obtain a hydrogel dressing; Step 3, mixing the hydrogel dressing prepared in step 2 with the antibacterial and repair-promoting microparticles to obtain a hydrogel dressing mixture; Step 4: Evenly mix the cactus mucus and costus lactone, and evenly spray them on the surface of the hydrogel dressing mixture prepared in step 3. After spraying, place them at 23-28° C. under ventilation conditions for 3-4 hours to obtain a hydrogel antibacterial wound dressing.

9. The method for preparing the hydrogel antibacterial wound dressing according to claim 8, characterized in that: In step 1, the weight ratio of the ultrapure water to the pig blood vessel is 1.3-1.5:1.

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