Environment-friendly efficient breeding feed for hermetia illucens and application of environment-friendly efficient breeding feed

Abstract

The invention discloses an environment-friendly high-efficiency breeding feed for hermetia illucens and application of the environment-friendly high-efficiency breeding feed. The hermetia illucens breeding feed comprises livestock and poultry manure and market wastes, the market waste comprises first waste and second waste, the first waste is stevia rebaudiana straw, and the second waste is soybean curb residue, fish viscera or chicken slaughter house waste. The efficient breeding feed for the hermetia illucens can remarkably improve the productivity of a hermetia illucens system, effectively reduces environmental gas emission in a biotransformation process, and is beneficial to synergistic interaction of environmental and economic benefits. Preferably, the first waste is stevia rebaudiana straw, the second waste is fish viscera, and the feed further comprises a compound probiotic liquid composed of aspergillus wendt, bacillus amyloliquefaciens and acinetobacter johnsonii, so that the productivity of a hermetia illucens system and the yield of polypide protein can be remarkably improved, environmental gas emission is reduced, and the environment pollution is reduced. The biotransformation rate, the waste reduction rate, the fat productivity and the insect sand seed germination index are improved.

Description

Technical Field

[0001] The invention relates to the technical field of resource utilization of organic waste and black soldier fly breeding, and in particular to a black soldier fly breeding feed capable of increasing production and reducing emissions and application thereof. Background Art

[0002] The rapid increase in the global population has led to a strong demand for animal products, especially poultry meat and eggs, which have high production efficiency and economy. Studies have shown that by 2050, the global demand for poultry and eggs will reach 283 million tons. China produces more than 40% of the world's eggs and has become the world's largest egg producer and consumer. Nearly 200 million tons of chicken manure are produced each year and cannot be effectively utilized. Traditional methods such as direct return to the field and simple fermentation will cause serious environmental pollution problems (odor nuisance, disease transmission and groundwater pollution), and nutrients cannot be fully utilized. Therefore, there is an urgent need to develop efficient and environmentally friendly treatment methods.

[0003] Black soldier fly ( Hermetia illucens L. ), also known as the bright-spotted flat-horned soldier fly, is a resource insect with strong environmental adaptability, high conversion efficiency and no disease transmission. Its larvae can quickly degrade and reduce chicken manure, and produce insect protein rich in protein and fat as animal feed. The residue can also be used as organic fertilizer or soil conditioner. However, due to the high moisture content and low nutrient content of chicken manure, the bioconversion rate of chicken manure consumed by black soldier fly larvae is only about 10%, resulting in a decrease in biomass yield and quality.

[0004] There are a large number of market wastes in rural areas of my country that are not properly handled, causing serious environmental pollution. However, these wastes (such as tail vegetables, tofu dregs, fish viscera, expired food and slaughter waste) are rich in cellulose, protein and (or) fat nutrients, and can be effectively used as nutritional supplements for black soldier fly feed. However, current research mainly focuses on the development of high-nitrogen feeds to improve the yield and quality of insects, but ignores the environmental problem that the conversion process may aggravate the emission of nitrogen-containing gases. Therefore, we aim to develop black soldier fly feeds with balanced nutrition, give full play to the advantages of material heterogeneity and aquaculture substrate structure adjustment achieved by a combination of multiple organic wastes, so as to achieve the synergistic progress of increased production and reduced environmental gas emissions, and provide technical support for the resource utilization and nutrient recovery of agricultural organic wastes in my country. Summary of the invention

[0005] In view of the above-mentioned prior art and its shortcomings, the purpose of the present invention is to provide a black soldier fly breeding feed and its application that has both the effects of increasing production and reducing emissions, so as to solve the problems of low yield and high emissions in the biological transformation process of black soldier flies, and effectively achieve a win-win situation of environmental and economic benefits.

[0006] The present invention provides a black soldier fly breeding feed, which includes livestock and poultry excrement and market waste; The market waste includes first waste and second waste, wherein the first waste is stevia stalks, and the second waste is tofu dregs, fish viscera or chicken slaughterhouse waste. As an example, the stevia stalks are naturally air-dried and then crushed and passed through a 60-mesh sieve.

[0007] In the above-mentioned black soldier fly breeding feed, the dry weight ratio of the livestock and poultry manure, the first waste and the second waste is 2:1:1; and / or, The livestock and poultry excrement is chicken excrement.

[0008] In the above-mentioned black soldier fly breeding feed, it is further preferred that the first waste is stevia straw, the second waste is fish viscera, and the feed also includes an effective viable bacteria count ratio of (0.37×10 8 ~0.42×10 8 ): (1.65×10 8 ~2.20×10 8 ): (0.73 × 10 8 ~0.92×10 8 Aspergillus wenti Aspergillus wentii )、Bacillus amyloliquefaciens( Bacillus amyloliquefaciens ) and Acinetobacter johnsonii ( Acinetobacter johnsonii ) The antagonism experiment confirmed that the mixed use of the strains had no antagonistic effect and had a synergistic effect.

[0009] As an example, the Aspergillus wenti Aspergillus wentii ) is numbered as CICC 40227, and the Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) is numbered as CICC 20604, the Acinetobacter johnsonii ( Acinetobacter johnsonii ) is numbered CICC 24792.

[0010] In the above-mentioned black soldier fly breeding feed, further, the livestock and poultry feces account for 50% of the total dry basis weight of the feed, the stevia straw accounts for 25% of the total dry basis weight of the feed, and the fish viscera accounts for 25% of the total dry basis weight of the feed; the total viable count of the composite probiotic bacterial liquid is ≥2.75×10 8 cfu / mL; the probiotic solution accounts for 1% to 3% (such as 3%) of the total mass of the feed; and / or, The method for preparing the composite probiotic bacterial liquid comprises the following steps: Aspergillus wentii )、Bacillus amyloliquefaciens( Bacillus amyloliquefaciens ) and Acinetobacter johnsonii ( Acinetobacter johnsonii) is inoculated into the enzyme production medium for cultivation to obtain the composite probiotic bacterial liquid. Further preferably, the enzyme production culture conditions are as follows: culture temperature is 30°C, initial pH is 7.0, seed liquid inoculation amount is 8%, rotation speed is 120 r / min, and culture time is 4 d.

[0011] As an example, the composition of the enzyme-producing culture medium is as follows: CMC-Na 5.0 g / L, (NH4)2SO41.4 g / L, MgSO4·7H2O 0.3 g / L, KH2PO42.0 g / L, CaCl20.3 g / L, FeSO4·7H2O 0.005 g / L, MnSO40.0016 g / L, ZnSO4·7H2O 0.0014 g / L, CoCl20.002 g / L, peptone 5 g / L, water as solvent, pH neutral.

[0012] The present invention optimizes the culture conditions of different bacterial liquids and screens the optimal temperature, pH, inoculation amount, culture speed and culture time. The results show that the optimal enzyme production culture conditions of the high-efficiency composite bacterial liquid are as follows: temperature 30°C, pH 7, inoculation amount 8%, 120 r / min, 4 d. Under this condition, the enzyme activity of the strain is the strongest, and the cellulase and protease yields reach 80.84±3.01 U / mL and 93.77±1.89 U / mL, respectively.

[0013] The present invention further provides the use of any one of the black soldier fly breeding feeds (excluding the composite probiotic solution) in at least one of the following A1) to A3): A1) Increase the production capacity of the black soldier fly system; A2) Improve the bioconversion rate of black soldier fly farming; A3) Reducing environmental gas emissions, including greenhouse gases and ammonia.

[0014] The present invention also provides a method for breeding black soldier flies, comprising the following steps: inoculating black soldier fly larvae into any of the above-mentioned black soldier fly breeding feeds (excluding the composite probiotic solution), breeding black soldier flies, and collecting insect bodies and insect sand after the breeding is completed. The method has at least one of the effects of A1) to A3).

[0015] In the above method, before the inoculation step, the total carbon-nitrogen ratio of the feed is adjusted to 20, and the moisture content is 70% to 75%. Furthermore, a carbon-containing material can be selected to adjust the total carbon-nitrogen ratio, such as sawdust.

[0016] In the above method, as an example, the black soldier fly larvae are black soldier fly larvae of several days old, such as 3 to 5 days old; the inoculation amount of the black soldier fly larvae is 500 / kg feed; the breeding is carried out in a light-proof open culture under a constant temperature of 20°C and a constant humidity of 70%.

[0017] The present invention further provides the use of any of the above preferred black soldier fly breeding feeds (comprising the composite probiotic bacterial liquid) in at least one of the following B1) to B8): B1) Increase the production capacity of the black soldier fly system; B2) Increase the protein production of insects; B3) reducing environmental gas emissions, wherein the environmental gases are greenhouse gases and ammonia; B4) Increase the growth rate of black soldier flies; B5) Improve the bioconversion rate of black soldier fly farming; B6) Improve waste reduction rates in black soldier fly farming; B7) Improve the fat productivity of black soldier flies; B8) Improve the germination index of insect sand seeds.

[0018] The present invention further provides a method for breeding black soldier flies, comprising the following steps: inoculating black soldier fly larvae into any of the above-mentioned preferred feeds, breeding black soldier flies, and collecting insect bodies and insect sand after breeding. The method has at least one of the effects of B1) to B8).

[0019] In the above method, before the inoculation step, sawdust is used to adjust the total carbon-nitrogen ratio of the feed to 20 and the moisture content to 70% to 75%; further, carbon-containing materials can be selected to adjust the total carbon-nitrogen ratio, such as sawdust.

[0020] In the above method, as an example, the black soldier fly larvae are black soldier fly larvae of several days old, such as 3 to 5 days old; the inoculation amount of the black soldier fly larvae is 500 / kg feed; the breeding is carried out in a light-proof open culture under a constant temperature of 20°C and a constant humidity of 70%.

[0021] The present invention has the following beneficial effects: (1) The present invention first adds any single market waste such as broccoli tails, stevia stalks, fish viscera, chicken slaughterhouse waste, tofu dregs, peanut and sesame food factory scraps, etc. to fresh chicken manure, and screens out single market waste with good gas emission reduction effect or insect body production increase effect in the process of black soldier flies converting chicken manure. Further, on the basis of chicken manure, stevia stalks are compounded with tofu dregs, fish viscera or chicken slaughterhouse waste to feed black soldier flies, and it is found that the production capacity of the black soldier fly system can be further improved, and ammonia emissions and greenhouse gas emissions in the conversion process can be reduced.

[0022] (2) The present invention further feeds black soldier flies with a compound mixture of chicken manure, stevia straw, fish viscera and a compound probiotic solution, which can significantly increase the production capacity and insect protein yield of the black soldier fly system, and reduce ammonia emissions and greenhouse gas emissions during the conversion process. The specific performance is as follows: 1) The black soldier fly high-efficiency breeding feed includes fresh chicken manure, stevia straw, fish viscera and a compound probiotic solution, among which our organic materials are all biomass waste with high yield but low utilization rate, and are different from traditional livestock and poultry manure and food waste, which significantly exert the heterogeneity of protein, fat and cellulose nutritional components, effectively improve the production capacity of the black soldier fly system through the interaction of nutrients, and reduce environmental gas emissions during the conversion process; 2) The compound probiotic solution is prepared in equal proportions using Aspergillus niger, Bacillus amyloliquefaciens and Acinetobacter johnsonii. The bacterial liquid not only has no antagonistic effect between the strains, but can also synergistically improve the decomposition and fermentation efficiency of the substrate. At the same time, it brings into play the degradation and conversion capabilities of different strains for substrate proteins, fats, lignin, cellulose and other substances, providing sufficient activated nutrients for the indigenous microbial community and larvae, and improving the nitrogen retention capacity in the substrate. It has a positive regulatory effect on the intestines of black soldier flies and substrate microorganisms, reducing the emission of gases such as methane, ammonia, and nitrous oxide, making the feed environmentally friendly and highly efficient, and providing help to improve the development of black soldier flies and even insect biotransformation in my country. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The figure shows the yield and greenhouse gas emissions of feeding black soldier flies with different single market wastes combined with chicken manure in Example 1 of the present invention.

[0024] Figure 2 It shows the yield and bioconversion efficiency of black soldier flies fed with the high-efficiency compound breeding feed in Example 2 of the present invention.

[0025] Figure 3 Shown is a comparison of ammonia and greenhouse gas emissions from feeding black soldier flies with the high-efficiency compound breeding feed in Example 2 of the present invention.

[0026] Figure 4 Shown are the results of the antagonism experiment of different probiotics in Example 3 of the present invention.

[0027] Figure 5 The figure shows the yield, protein yield and greenhouse gas emissions of black soldier flies fed with the high-efficiency compound breeding feed after adding different probiotics in Example 3 of the present invention. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0029] The methods used in the following examples, unless otherwise specified, are all conventional methods, carried out according to the techniques or conditions described in the literature in the art or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial sources.

[0030] The broccoli tails in the following embodiments come from the vegetable market and the roadside of the picking place, the chicken slaughterhouse waste comes from the laying hen and chicken farm, and the tails and chicken slaughterhouse waste need to be crushed to about 3 cm by machinery for standby use; the tofu dregs come from the mill or soy product processing factory, and the fish viscera are various discarded fish wastes from the market fish stall, without additional treatment; the fresh chicken manure is obtained from the conveyor belt breeding farm, and no pesticides are sprayed. Stevia stalks are naturally air-dried and crushed, passed through a 60-mesh sieve, and set aside. Black soldier fly eggs were purchased from Zhengzhou Kuocheng Ecological Agriculture Technology Co., Ltd.

[0031] Example 1 In this embodiment, black soldier flies are fed with a feed consisting of fresh chicken manure and single market waste to screen single market waste with better gas emission reduction effect and insect body yield increase effect.

[0032] The single market wastes used are: broccoli leaves (CMV), stevia straw (CMS), fish offal (CMO), chicken slaughterhouse waste (CMC), tofu dregs (CMT), and peanut and sesame food factory scraps (CMF).

[0033] The specific steps are as follows: (1) Hatching larvae: Incubate purchased eggs at 30°C and 30% relative humidity, transfer to coarse wheat bran and culture in the dark until they are 3 to 5 days old.

[0034] (2) Raw material mixing: Mix fresh chicken manure and various single market wastes in a dry basis weight ratio of 1:1, adjust the total carbon-nitrogen ratio to 20:1 with sawdust, control the moisture content to 70%-75%, and spread it on a 1 m 2 In cement pool.

[0035] (3) Larvae inoculation and index determination: 3-5 day old black soldier fly larvae were added at a dosage of 500 / kg and placed in an artificial climate chamber. They were cultured in an open environment away from light at a constant temperature of 20°C and a constant humidity of 70%. Greenhouse gas detection was performed on each treatment every two days using a dynamic chamber method. The piles were then turned to improve the permeability of the pile and the activity of the larvae. A small amount of water was added to maintain a suitable moisture content that was conducive to the growth of the larvae. The experiment was terminated when 50% of the larvae reached the prepupa stage. After screening the black soldier fly bodies (separating the black soldier flies from the worm sand residue), the bodies and worm sand were weighed separately, and the total body production, total productivity and greenhouse gas emissions were calculated.

[0036]

[0037] Figure 1 The total productivity and greenhouse gas emissions of black soldier flies fed with chicken manure combined with different single market wastes were compared. The results showed that compared with the pure chicken manure control group, the total insect production of black soldier flies fed with chicken manure combined with different single market wastes increased by 3.22%-69.55%, and the greenhouse gas emissions during the conversion process decreased by 2.70%-44.21%. Among them, stevia straw had the best effect in reducing greenhouse gas emissions during the conversion process, and tofu dregs, fish viscera and chicken slaughterhouse waste had a good effect in increasing insect production. Subsequently, the single market wastes with better gas emission reduction and insect production increase effects were mixed to prepare composite breeding feed.

[0038] Example 2 In this example, the single market waste with good gas emission reduction effect and insect yield increase effect screened out in Example 1 was mixed to prepare a high-efficiency composite breeding feed, and its production increase and emission reduction effect was investigated, including the effects of feeding black soldier flies with high-efficiency breeding feed and traditional chicken manure feeding on the production capacity and environmental gas emissions of the black soldier fly bioconversion system.

[0039] The compositions of the high-efficiency compound breeding feed are: chicken manure + stevia straw (CMS) (1:1), chicken manure + stevia straw + tofu dregs (CMST) (2:1:1), chicken manure + stevia straw + fish viscera (CMSO) (2:1:1), and chicken manure + stevia straw + chicken slaughterhouse waste (CMSC) (2:1:1).

[0040] The specific steps are as follows: (1) The methods of raw material pretreatment, larvae hatching and raw material mixing are the same as those in Example 1. The addition amount of each component in different treatments is equal to the dry weight ratio, and the raw materials are thoroughly mixed with a shovel.

[0041] (2) Larvae inoculation and index determination: The inoculation method and index determination were the same as in Example 2, and the calculation was performed according to the following formula: bioconversion efficiency (%) = total dry weight of black soldier fly bodies after the culture was completed / (dry weight of the initial added substrate - dry weight of the remaining insect sand after the culture was completed) × 100%. The ammonia emission rate and cumulative emission during the conversion process were monitored using the boric acid absorption method.

[0042] Figure 2 It shows the comparison of the yield and bioconversion efficiency of black soldier flies fed with high-efficiency compound breeding feed. Figure 3 Comparison of ammonia and greenhouse gas emissions from black soldier flies fed a high-efficiency composite aquaculture diet.

[0043]

[0044] Depend on Figure 2 and Figure 3 It can be seen that the high-efficiency compound breeding feed of the present invention can significantly improve the total productivity of black soldier flies by 45.37%-92.24% compared with the control group of pure chicken manure and stevia straw, and the bioconversion rate is increased by 6.35%-93.55%. Among them, the chicken manure + stevia straw + fish viscera (CMSO) has the best comprehensive effect of increasing production and reducing emissions. Compared with other compound breeding feed groups, ammonia emissions are significantly reduced by 10.75%-21.68%, and greenhouse gas emissions are reduced by 3.86%. This feed will be selected for further research.

[0045] Example 3 In this example, different probiotics are further added to the high-efficiency compound breeding feed in Example 2, and the effect of increasing production and reducing emissions is investigated, including the effect of feeding black soldier flies with high-efficiency breeding feed added with different probiotics on the production capacity, insect body quality and environmental gas emissions of the black soldier fly bioconversion system.

[0046] The different probiotics are: Aspergillus wente ( Aspergillus wentii ) bacterial solution (S1), Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) bacterial solution (S2), Acinetobacter johnsonii ( Acinetobacter johnsonii ) bacterial solution (S3), Aspergillus wentifolia ( Aspergillus wentii ) + Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens )+Acinetobacter johnsonii ( Acinetobacter johnsonii ) Mixed bacterial solution (Mix). Among them, the strain number of Aspergillus wentei is CICC 40227, the strain number of Bacillus amyloliquefaciens is CICC 20604, and the strain number of Acinetobacter johnsonii is CICC 24792, all of which were purchased from China Industrial Microbiological Culture Collection Center (CICC).

[0047] The specific steps are as follows: (1) The methods of raw material pretreatment, larvae hatching, raw material mixing, larvae inoculation and index determination are the same as those in Example 1. The composite breeding feed used is the optimally treated chicken manure + stevia straw + fish viscera (CMSO) obtained in Example 2, and the average protein content of the collected insect bodies is determined by the Kjeldahl method.

[0048] (2) Preparation and application of bacterial solution: Aspergillus wentei ( Aspergillus wentii ), Bacillus amyloliquefaciens and Acinetobacter johnsonii, and conventional PDA and LB media were used for strain subculture and propagation.

[0049] The three stable strains were streaked in pairs on PDA and BPM solid culture media. When the strains grew into stripes, the inhibition zones were observed at the intersections of different strains to test whether the strains could be used to construct composite bacterial strains. Figure 4 It can be seen that the strains all grow well at the intersection, without antagonism, and can be combined and compounded.

[0050] Aspergillus wenti Aspergillus wentii ) was inoculated into potato (PDA) liquid medium (HB0233-8 potato liquid medium from Haibo Biotechnology Co., Ltd., prepared according to the concentration in the instruction manual), Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) and Acinetobacter johnsonii ( Acinetobacter johnsonii ) was inoculated into beef extract peptone (BPM) liquid medium (Haibo Biotechnology Co., Ltd., HB9141 beef extract peptone medium, prepared according to the concentration in the instruction manual). The first generation strain was obtained by shaking at 28°C and 120 rpm for 24 h, and the activated strains were inoculated into liquid growth medium (CMC-Na 15.0 g, NH4NO3 1.0 g, MgSO4·7H2O 0.5 g, KH2PO4 1.0 g, NaCl 1.0 g, peptone 1.0 g, yeast extract powder 1.0 g, distilled water 1 L, pH neutral) shake flasks at 25°C and 120 rpm for 24 h to obtain seed solutions (S1, S2, S3). The seed solutions of each strain were mixed in equal volume ratios (each accounting for 33.3%) to obtain a composite seed solution (Mix); Each sub-liquid was inoculated into a liquid enzyme-producing medium (CMC-Na 5.0 g, (NH4)2SO4 1.4 g, MgSO4·7H2O 0.3 g, KH2PO4 2.0 g, CaCl2 0.3 g, FeSO4·7H2O 0.005 g, MnSO4 0.0016 g, ZnSO4·7H2O 0.0014 g, CoCl2 0.002 g, peptone 5 g, distilled water 1 L, pH neutral) at an inoculum volume of 8% and cultured at 30°C and an initial pH of 7.0 for 4 d (120 r / min) to prepare the corresponding inoculum solution. The total viable count of the single bacterial solution S1 was 1.12-1.27×10-1 8 cfu / mL, the total viable count of single bacterial solution S2 was 2.89~4.07×10 8 cfu / mL, and the total viable count of single bacterial solution S3 was 2.57-2.96×10 8 cfu / mL, the composite bacterial solution Mix contains 0.37~0.42×10 8 cfu / mL of Aspergillus wenti Aspergillus wentii )、1.65~2.20×10 8 Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) and 0.73~0.92×10 8 Acinetobacter johnsonii ( Acinetobacter johnsonii ), total viable count ≥ 2.75 × 10 8 cfu / mL.

[0051] The liquid bacterial solution was weighed according to 3% of the total substrate mass and diluted with sterile physiological saline, and then the different bacterial solutions were evenly sprayed on the substrate surface before inoculating the insects. The remaining steps were the same as those in Example 2, including hatching larvae, mixing raw materials, inoculating larvae, and determining indicators.

[0052] Figure 5 Comparison of yield, protein production and greenhouse gas emissions of black soldier flies fed with different probiotics added to high-efficiency compound aquaculture feed. Figure 5 It can be seen that compared with other probiotic groups, the total productivity of insects treated with Mix was significantly increased by more than 8.26%, the protein productivity of insects was significantly increased by more than 16.91%, and greenhouse gas emissions were significantly reduced by more than 3.50%.

[0053]

[0054] Table 1 compares the properties of black soldier fly growth rate, bioconversion rate, waste reduction rate, fat productivity, total nitrogen content of insect sand and insect sand seed germination index after adding different probiotics to high-efficiency compound breeding feed.

[0055]

[0056]

[0057] As can be seen from Table 1, the growth rate of the insect body in the Mix treatment increased by more than 4.50%, the bioconversion rate and waste reduction rate increased by more than 4.91% and 2.68% respectively, and the fat productivity of the insect body increased significantly by more than 12.75%. At the same time, the total nitrogen content in the insect sand residue after the insect body was harvested was also maintained at a high level of about 2.75%, and the seed germination index was much higher than the requirement of ≥70% in the national organic fertilizer industry standard NY / T525-2021, achieving a synergistic improvement in the production capacity and emission reduction of the black soldier fly system, and the properties of the Mix treatment group were better than those of the S1, S2 and S3 treatment groups using only one of the strains, which further demonstrated the synergistic effect of the three strains.

[0058] The above examples show that the environmentally friendly black soldier fly efficient breeding feed of the present invention can significantly improve the production capacity of the black soldier fly system and effectively reduce the environmental gas emissions during the bioconversion process, which is beneficial to the synergistic enhancement of environmental and economic benefits.

[0059] The present invention has been described in detail above. For those skilled in the art, without departing from the purpose and scope of the present invention, can implement the present invention in a wider range under equivalent parameters, concentrations and conditions. Although the present invention provides specific embodiments, it should be understood that the present invention can be further improved. In a word, according to the principle of the present invention, the application is intended to include any changes, uses or improvements to the present invention, including departing from the disclosed scope in the application and the changes made with conventional techniques known in the art.

Claims

1. A black soldier fly breeding feed, characterized in that: Including livestock and poultry manure and market waste; The market waste includes first waste and second waste, wherein the first waste is stevia straw, and the second waste is tofu dregs, fish viscera or chicken slaughterhouse waste.

2. The black soldier fly breeding feed according to claim 1, characterized in that: The dry weight ratio of the livestock and poultry manure, the first waste and the second waste is 2:1:1; and / or, The livestock and poultry excrement is chicken excrement.

3. The black soldier fly breeding feed according to any one of claims 1-2, characterized in that: The first waste is stevia stalks, the second waste is fish viscera, and the feed also includes an effective live bacteria count ratio of (0.37×10 8 ~0.42×10 8 ): (1.65×10 8 ~2.20×10 8 ): (0.73 × 10 8 ~0.92×10 8 Aspergillus wenti Aspergillus wentii )、Bacillus amyloliquefaciens( Bacillus amyloliquefaciens ) and Acinetobacter johnsonii ( Acinetobacter johnsonii ) composed of a composite probiotic liquid.

4. The black soldier fly breeding feed according to claim 3, characterized in that: The livestock and poultry excrement accounts for 50% of the total dry basis weight of the feed, the stevia straw accounts for 25% of the total dry basis weight of the feed, and the fish viscera accounts for 25% of the total dry basis weight of the feed; the total viable bacterial count of the composite probiotic solution is ≥2.75×10 8 cfu / mL; the probiotic solution accounts for 1% to 3% of the total mass of the feed; and / or, The method for preparing the composite probiotic bacterial liquid comprises the following steps: seed liquids of Aspergillus wentii, Bacillus amyloliquefaciens and Acinetobacter johnsonii are respectively inoculated into enzyme-producing culture medium for culturing to obtain the composite probiotic bacterial liquid; preferably, the enzyme-producing culture conditions are as follows: culture temperature of 30°C, initial pH of 7.0, seed liquid inoculation amount of each strain of 8%, rotation speed of 120 r / min, and culture time of 4 d.

5. Use of the black soldier fly breeding feed according to any one of claims 1-2 in at least one of the following A1)-A3): A1) Increase the production capacity of the black soldier fly system; A2) Improve the bioconversion rate of black soldier fly farming; A3) Reducing environmental gas emissions, including greenhouse gases and ammonia.

6. A method for breeding black soldier flies, characterized in that: The method comprises the following steps: inoculating black soldier fly larvae into the feed described in any one of claims 1 to 2, breeding black soldier flies, and collecting insect bodies and insect sand after the breeding is completed.

7. The method according to claim 6, characterized in that: Before the inoculation step, the total carbon-nitrogen ratio of the feed is adjusted to 20, and the moisture content is adjusted to 70% to 75%.

8. Use of the black soldier fly breeding feed according to any one of claims 3 to 4 in at least one of the following B1) to B8): B1) Increase the production capacity of the black soldier fly system; B2) Increase the protein production of insects; B3) reducing environmental gas emissions, wherein the environmental gases are greenhouse gases and ammonia; B4) Increase the growth rate of black soldier flies; B5) Improve the bioconversion rate of black soldier fly farming; B6) Improve waste reduction rates in black soldier fly farming; B7) Improve the fat productivity of black soldier flies; B8) Improve the germination index of insect sand seeds.

9. A method for breeding black soldier flies, characterized in that: The method comprises the following steps: inoculating black soldier fly larvae into the feed described in any one of claims 3-4, breeding black soldier flies, and collecting insect bodies and insect sand after the breeding is completed.

10. The method according to claim 9, characterized in that: Before the inoculation step, the total carbon-nitrogen ratio of the feed is adjusted to 20, and the moisture content is adjusted to 70% to 75%.

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