Compound microbial agent as well as preparation method and application thereof

Abstract

The invention relates to a compound microbial agent. The compound microbial agent mainly comprises the following components: 70-80 parts of a microbial solution, 2-5 parts of a microbial accelerant and 20-30 parts of organic and inorganic nutrient substances. The microbial bacterial liquid is prepared from bacillus subtilis, paenibacillus mucilaginosus, nitrogen-fixing bacteria and pichia pastoris powder; the microbial accelerant comprises one or more of rare earth elements, protease, amino acid and betaine. Four beneficial microorganisms are subjected to combined fermentation by adopting innovative strain fermentation equipment, and a microbial accelerant and organic and inorganic nutrient substances are added for compounding, so that the effects of the beneficial microorganisms are further promoted, the growth and development of plants can be promoted, the stress resistance of the plants can be improved, and the yield of the plants is increased. The requirement of soil for chemical nitrogen elements is reduced, soil microbial communities are enriched, a healthier ecological system is formed, and soil fertility can be maintained for a long time.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fertilizers, and in particular to a composite microbial agent and a preparation method and application thereof. Background Art

[0002] As the world pays more attention to sustainable development and environmental protection, the use of microorganisms to improve soil quality, promote plant growth and control environmental pollution is gaining more and more attention. Microorganisms play an important role in decomposing organic matter and recycling nutrients in nature, so the development of efficient microbial preparations is of great significance for improving agricultural production efficiency, reducing the use of chemical pesticides and protecting the ecological environment.

[0003] Composite microbial agents are microbial preparations made from two or more non-antagonistic microbial strains. It is a new type of biological disease-resistant fertilizer. Composite microbial agents generally contain a large number of beneficial live bacteria and a variety of natural fermentation active substances. They multiply in the root zone soil of crops to form dominant microbial flora that are beneficial to crop growth, regulate the root zone nutritional environment of crops, and restore the soil microecological balance. In addition, they can also produce various plant growth hormones in the process of their life activities to stimulate plant growth. At the same time, they also have the effect of resisting certain pathogenic microorganisms and inhibiting diseases. They are deeply loved by farmers.

[0004] There are a lot of mixed microbial agents on the market. The strains and functions of the microbial products are single, and the number of live bacteria in the agents is insufficient. In addition, the strain fermentation equipment on the market has the following problems: it uses a single agitator to stir the strains and fermentation liquid, and the stirring effect is poor. It is difficult to fully mix different strains and fermentation materials, and the stirring blades are difficult to mix and stir the composite microbial agents near the inner wall of the tank, and the strains are easily deposited, which affects the effect of the fermentation compounding.

[0005] Therefore, the prior art needs to be further improved. Summary of the invention

[0006] The present invention aims to solve the above problems and provides a composite microbial agent, which mainly includes the following components: 70-80 parts of microbial liquid, 2-5 parts of microbial promoter, and 20-30 parts of organic and inorganic nutrients. Preferably, it includes 74 parts of microbial liquid, 4 parts of microbial promoter, and 22 parts of organic and inorganic nutrients. The effective viable count of the composite microbial agent is ≥ 500 million / ml.

[0007] Furthermore, the microbial liquid includes: Bacillus subtilis, Paenibacillus jelly, nitrogen-fixing bacteria, and powdery Pichia pastoris; The microbial liquid is prepared by compounding Bacillus subtilis, Paenibacillus gelatinus, nitrogen-fixing bacteria and powdery Pichia pastoris in a mass ratio of 1:1:0.5:0.2.

[0008] Further preferably, the Bacillus subtilis, with a deposit number of ACCC62258, was purchased from the China Agricultural Microorganism Culture Collection Administration Center. The Paenibacillus mucilaginosus, with a deposit number of ACCC 01434, was purchased from the China Agricultural Microorganism Culture Collection Administration Center. The nitrogen-fixing bacteria is Azotobacter chroococcum, with a deposit number of ACCC 10096, purchased from the China Agricultural Microorganism Culture Collection Administration Center. The Pichia farinosa, with a deposit number of ACCC 21153, was purchased from the China Agricultural Microorganism Culture Collection Administration Center.

[0009] Furthermore, the microbial promoter includes: one or more of rare earth elements, protease, amino acid, and betaine. Among them, protease can be used to decompose complex organic fertilizers into simpler forms for easy absorption and utilization by microorganisms; amino acids include serine and lysine, and betaine can protect cell structures under extreme environmental conditions and enhance plant resistance.

[0010] Furthermore, the organic and inorganic nutrients include: 5-10 parts of potassium dihydrogen phosphate, 2-7 parts of calcium chloride, 3-5 parts of zinc sulfate, 10-12 parts of humic acid, and 50-80 parts of water.

[0011] Based on the same invention, the present invention also provides a method for preparing the above-mentioned composite microbial agent, and the preparation method of the composite microbial agent comprises the following steps: S1: Preparation of bacterial activation, preparation of microbial promoters, and preparation of organic and inorganic nutrients; S2: inoculating the activated strains into the strain fermentation equipment according to proportion for fermentation; S3: Add microbial promoters and organic and inorganic nutrients to obtain a composite microbial agent.

[0012] Based on the above technical solution, the strain fermentation equipment used in step S2 includes a fermentation tank, which is connected to a stirring device, the stirring device is connected to a lifting device connected to the fermentation tank, and the lifting device is connected to a supporting culture device connected to the fermentation tank; an air inlet pipe is provided on the supporting culture device, and an air outlet device is provided on the fermentation tank.

[0013] Based on the above technical solution, the fermentation tank includes a U-shaped tank body and a tank cover detachably connected to the top of the U-shaped tank body; a temperature control interlayer is arranged in the U-shaped tank body, and the temperature control interlayer is connected to a temperature control inlet and a temperature control outlet fixed at both ends of the U-shaped tank body.

[0014] Based on the above technical solution, the temperature control inlet and the temperature control outlet at both ends of the U-shaped tank body are respectively rotatably connected to the two side plates of the equipment bracket, and the two ends of the bottom of the U-shaped tank body are respectively connected to the bottom plate of the equipment bracket through a supporting spring body.

[0015] Furthermore, the stirring device includes a motor, which is installed on the fermentation tank. The motor is connected to a drive shaft that is rotated in the fermentation tank. The drive shaft penetrates into the fermentation tank and is equipped with one or more flipping blades.

[0016] Furthermore, the driving shaft passes through the shaft body outside the fermentation tank and is keyed to the driving wheel, the driving wheel is connected to the input wheel in the jacking device through a driving belt, the input wheel is keyed to the worm, the worm rotated on the force-bearing bracket is meshed with the worm wheel fixed on the output shaft, the output shaft is rotatably connected to the fermentation tank through the force-bearing bracket, the output shaft is fixed in the eccentric hole of the eccentric jacking wheel, and the eccentric jacking wheel is driven to connect to the load-bearing culture device.

[0017] Furthermore, the load-bearing culture device includes a load-bearing gear fixed on the force-bearing horizontal axis, and the lower end of the load-bearing gear is meshed with the driving wheel; the force-bearing horizontal axis is connected to two linkage frames, and the two linkage frames are connected to the upper ends of two lifting vertical plates. The middle parts of the two lifting vertical plates are sealed and slid on the fermentation tank, and the lower ends of the two lifting vertical plates are inserted into the fermentation tank and connected to a U-shaped load-bearing frame arranged in a U-shaped groove inside the fermentation tank; the rectangular chamber arranged inside the lifting vertical plate is connected to the U-shaped chamber arranged inside the U-shaped load-bearing frame, and the U-shaped chamber is connected to multiple medium ports arranged on the U-shaped surface inside the U-shaped load-bearing frame, and ultrafiltration nets are provided in the multiple medium ports; the rectangular chamber of the lifting vertical plate is connected to the air inlet pipe that is threaded and sealed and connected to the outer side of the lifting vertical plate.

[0018] Furthermore, the supporting culture device also includes a two-way water pump, and the two ends of the two-way water pump are respectively fixed to a horizontal pipeline and a vertical pipeline. The horizontal pipeline is fixed on the inner side of the lifting vertical plate and is connected to the rectangular chamber of the lifting vertical plate; the middle part of the longitudinal pipeline is sealed and slid on the fermentation tank, and the lower end of the longitudinal pipeline is inserted into the fermentation tank.

[0019] Furthermore, a drain pipe is fixedly connected to the side of the longitudinal pipeline, and a pipe plug is connected to the outer end of the drain pipe through a threaded seal; a valve is provided on the longitudinal pipeline, and the valve is located below the drain pipe.

[0020] Furthermore, the air outlet device includes an air outlet straight pipe, the middle part of which is sealed and fixed in the exhaust hole at the top of the fermentation tank, the top of the air outlet straight pipe is sealably and slidably connected to a cylindrical sealing plug, the cylindrical sealing plug is connected to the lifting vertical shaft, the lower end of the lifting vertical shaft is fixed on the lifting disk, the lifting disk is slidably connected to the middle parts of multiple vertical sliding shafts, the tops of the multiple vertical sliding shafts are fixedly connected to an annular seat, the annular seat is connected to the top surface of the fermentation tank by bolts, and multiple air outlet compression springs fixed between the lifting disk and the annular seat are sleeved on the multiple vertical sliding shafts.

[0021] Furthermore, the top of the cylindrical sealing plug is fixedly connected to a limit plate, and the lower surface of the limit plate abuts against the air outlet straight pipe; the first air hole at the bottom of the cylindrical sealing plug and the second air hole on the outer side of the cylindrical sealing plug are both connected to the air cavity inside the cylindrical sealing plug, and the sealing sleeve coaxially fixedly connected on the lower surface of the limit plate is sealingly slidably fitted on the outer side of the air outlet straight pipe, and a plurality of third air holes are provided on the side of the sealing sleeve.

[0022] Based on the same invention, the present invention also provides the use of the composite microbial agent as described above and / or the composite microbial agent prepared by the preparation method as described above in promoting plant growth.

[0023] Furthermore, the dosage of the composite microbial agent is 3-10 L / mu.

[0024] Furthermore, the composite microbial agent can be applied by flushing, drip irrigation, root irrigation, foliar spraying, etc. The dilution multiple for root irrigation is 200-500 times; the dilution multiple for foliar spraying is 200-500 times; The invention has the following advantages: 1. The present invention is a liquid composite microbial agent, which contains four beneficial bacteria, among which brown ball nitrogen-fixing bacteria is an important autogenous nitrogen-fixing bacteria, which can fix nitrogen in the air without plant symbiosis, thereby providing an additional nitrogen source for the soil and reducing the demand for chemical nitrogen fertilizers. In addition, brown ball nitrogen-fixing bacteria can also secrete a variety of plant growth regulators such as cytokinins and indoleacetic acid (IAA), which help stimulate root development and enhance the efficiency of plant nutrient absorption. The introduction of brown ball nitrogen-fixing bacteria can enrich the soil microbial community, form a healthier ecosystem, and help maintain soil fertility in the long term.

[0025] 2. In addition, the liquid composite microbial agent of the present invention also includes powdered Pichia pastoris, which can decompose complex organic matter, thereby helping to improve the physical properties of the soil and improve soil aeration and water retention capacity. Enzymes and other secondary metabolites produced during yeast metabolism can help accelerate the decomposition of organic matter in the soil and release more mineral nutrients for plant absorption and utilization, such as phosphorus, potassium and other elements. By forming a symbiotic relationship with plant roots or indirectly affecting the structure of soil microbial communities, it helps to maintain a healthy and diverse soil ecosystem, thereby improving overall soil fertility. When preparing composite microbial fertilizers, powdered Pichia pastoris can also be used together with other beneficial microorganisms to act as a carrier or auxiliary ingredient to stabilize the product formula and ensure the effective survival rate and activity of other functional bacteria.

[0026] 3. The present invention also innovatively provides a composite microbial inoculant bacterial strain fermentation equipment, in which the stirring device inside is used for mixing and stirring, and can also cooperate with the lifting device to drive the carrying culture device to slide in the up and down directions, so that the carrying culture device drives the carried bacterial strains and fermentation liquid to shake, thereby improving the mixing uniformity of the bacterial strains and fermentation liquid, and the carrying culture device can control the fermentation liquid in the fermentation tank to circulate, thereby improving the effect of the fermentation liquid at different positions on the bacterial strain cultivation.

[0027] 4. The present invention adopts innovative bacterial fermentation equipment to jointly ferment four beneficial microorganisms, and adds microbial promoters and organic and inorganic nutrients for compounding, which further promotes the role of beneficial microorganisms, can promote plant growth and development, improve plant stress resistance, reduce the demand for chemical nitrogen elements in the soil, enrich soil microbial communities, form a healthier ecosystem, and is conducive to long-term maintenance of soil fertility. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only one embodiment of the present invention, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0029] Figure 1 A schematic diagram of a bacterial fermentation device from a first perspective provided in an embodiment of the present invention; Figure 2 A cross-sectional view of a bacterial fermentation device provided in an embodiment of the present invention Figure 1 ; Figure 3 A cross-sectional view of a bacterial fermentation device provided in an embodiment of the present invention Figure 2 ; Figure 4 A schematic diagram of a fermentation tank provided in an embodiment of the present invention; Figure 5 A cross-sectional view of a fermentation tank provided by an embodiment of the present invention; Figure 6 A schematic diagram of a device support provided by an embodiment of the present invention; Figure 7 A schematic diagram of a stirring device provided in an embodiment of the present invention; Figure 8 A schematic diagram of a lifting device provided in an embodiment of the present invention; Fig. 9 A partial diagram of a lifting device provided in an embodiment of the present invention; Fig.10 A schematic diagram of an air outlet device provided in an embodiment of the present invention; Fig.11 A partial diagram of an air outlet device provided in an embodiment of the present invention; Fig.12 A schematic diagram of a carrier culture device provided in an embodiment of the present invention; Fig.13 A cross-sectional view of a supporting culture device provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. The following embodiments are only for illustrating the present invention, and are not intended to limit the scope of the present invention in any way. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention. The experimental methods used in the present invention are conventional methods unless otherwise specified. The materials, reagents, etc. used in the present invention, unless otherwise specified, can be obtained from commercial channels. In addition, other terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art.

[0031] Example 1: Microbial culture screening pot experiment 1.1 Experimental Materials Test plants: Tomato ( Solanum lycopersicum L. ), the variety was Qianxi, purchased from Jiangsu Yimutian Vegetable Seedling Co., Ltd.; Test soil: taken from Gaotang County, Liaocheng City, Shandong Province.

[0032] 1.2 Experimental methods The pot experiment was conducted in flower pots with an inner diameter of 20 cm, an inner diameter of 14 cm and a height of 16 cm. Each pot contained 3 kg of soil. After the tomato seedlings were planted, the microbial solution was diluted 500 times and used for root irrigation. The experimental group settings are shown in Table 1: Table 1: Experimental group settings for Example 1

[0033] In the experimental settings in Table 1, 30 tomato seedlings were used for each treatment, and more than 3 groups of parallel repeated experiments were set up for each treatment.

[0034] 1.3 Measurement indicators Seven days after the tomato seedlings were planted, the indicators were measured, and 15-20 tomato seedlings with uniform growth were selected from each group to measure the experimental data. The height of the tomato plants was measured with a tape measure, and the stem diameter was measured with a vernier caliper.

[0035] 1.4 Experimental Data The test data are shown in Table 2.

[0036] Table 2: Experimental data of Example 1

[0037] 1.5 Experimental Results and Analysis The height and stem thickness of the plant can directly reflect the growth status of the plant. By analyzing the experimental data in Table 2, we can get: In the present application, the plant growth of the experimental treatments of T1-T9 with the addition of beneficial strains was significantly better than that of the control, especially the plant height of the T9 experimental group of the present application increased by 67.44% compared with the CK1 group, and the stem diameter increased by 72.68% compared with the CK1 group. Therefore, T9 was selected as the best microbial strain combination in the present application.

[0038] Example 2: Composite microbial agent small sample potted plant screening test 2.1 Experimental Materials Same as Example 1 2.2 Experimental methods Based on the combination of the T9 microbial liquid obtained in Example 1, microbial promoters and organic and inorganic nutrients were added to prepare composite microbial agent samples to verify the growth-promoting effect of the fertilizer samples. The microbial agent samples were prepared using conventional strain fermentation equipment and the strain fermentation equipment of the present application, and the fertilizer efficiency was compared.

[0039] Put 3kg of soil in each pot. After the tomato seedlings are planted, dilute the compound microbial agent 500 times and irrigate the roots.

[0040] The experimental group settings are shown in Table 3: Table 3: Experimental group settings of Example 2

[0041] In the experimental settings in Table 3, each treatment had 15 tomato seedlings, and each treatment had more than 3 groups of parallel repeated experiments.

[0042] 2.3 Test indicators 14 days after tomato planting, the indicators were measured, and 10 to 12 tomato seedlings with uniform growth were selected from each group to measure the experimental data. The height of the tomato plants was measured with a tape measure, and the stem diameter was measured with a vernier caliper.

[0043] 2.4 Experimental Data The test data are shown in Table 4.

[0044] Table 4: Experimental data of Example 2

[0045] 2.5 Experimental Results and Analysis The height and stem thickness of the plant can directly reflect the growth status of the plant. By analyzing the experimental data in Table 4, we can get: The plant growth of all treatment groups added with microbial promoters in Table 4 was significantly better than that of CK2 group. Among them, the plant height of group T12, which used the fermentation equipment of the present application, increased by 15.87% and the stem diameter increased by 14.37% compared with that of group CK2; while the plant height of group T15, which used conventional fermentation equipment, increased by 11.57% and the stem diameter increased by 9.31% compared with that of group CK2. The plant height of group T12 increased by 3.85% and the stem diameter increased by 4.62% compared with that of group T15.

[0046] Therefore, the treatment of group T12 had the best growth-promoting effect on tomato plants.

[0047] Example 3: Field experiment of composite microbial agent product (tomato) 3.1 Experimental Materials Test plants: Tomato ( Solanum lycopersicum L. ), the variety is Provence.

[0048] Test location: Tomato planting shed of farmers in Gaotang County, Liaocheng City, Shandong Province.

[0049] The control product is a liquid microbial inoculant product with a single strain of bacteria commonly used by farmers.

[0050] 3.2 Experimental methods The fertilizer samples of T12 and T15 in Example 2 were made into composite microbial agent products, and the composite microbial agent was drip-irrigated according to the experimental setting dosage. In the greenhouse, each ridge was about 0.02 mu. Two ridges were set for each treatment in the experiment, and more than three groups of repeated tests were set in the entire greenhouse.

[0051] The experimental group settings are shown in Table 5: Table 5: Experimental group settings for Example 3

[0052] 3.3 Test indicators Plant height, stem diameter, chlorophyll, and leaf thickness of tomatoes 7 days after planting; the first yield measurement, the second yield measurement, and the fruit weight of the second yield measurement.

[0053] Plant height (cm): Use a tape measure to measure from the surface soil to the highest point of the plant's main stem; Stem diameter (mm): The stem diameter was measured at the flattest part of the stem using a vernier caliper; Following up on the first two yield measurements, the yield data in the table refers to the yield of each ridge (about 0.02 mu); Single fruit weight: For each treatment, select fruits with uniform growth and weigh and count them, and repeat for more than 3 times.

[0054] 3.4 Experimental Data The experimental data are shown in Table 6.

[0055] Table 6: Experimental data of Example 3

[0056] 3.5 Experimental Results and Analysis From the experimental data analysis of Table 6, it can be obtained that compared with the conventional liquid microbial product on the market in the CK3 group, the product of the present invention in the T17 group increased plant height by 43.93%, stem diameter by 59.47%, the first yield measurement increased by 44.13%, the second yield measurement increased by 51.03%, and the single fruit weight increased by 44.16%.

[0057] In addition, compared with the microbial agent prepared by the conventional fermentation equipment of the T19 group, which used the composite microbial agent prepared by the strain fermentation equipment of the present application, the plant height of the T17 group of the present application increased by 11.42%, the stem thickness increased by 10.54%, the yield increased by 8.19% in the first measurement, increased by 6.82% in the second measurement, and the single fruit weight increased by 6.37%.

[0058] Example 4: Field experiment of composite microbial agent product (soybean) 4.1 Experimental Materials Test plants: soybean (Glycine max (L.) Merr.), variety Zhonghuang 35.

[0059] Test location: farmers' fields in Gaotang County, Liaocheng City, Shandong Province.

[0060] The control product is the base fertilizer product commonly used by farmers.

[0061] 4.2 Experimental methods The composite microbial agent was used as base fertilizer according to the experimental setting dosage. The experimental group settings are shown in Table 7: Table 7: Experimental group settings of Example 4

[0062] 4.3 Production data and analysis Table 8: Experimental data of Example 4

[0063] According to the experimental data analysis in Table 8, the composite microbial agent of the present application increased yield by 77.06% and 29.43% respectively when used at a dosage of 10L / mu (T21) compared with the conventional treatments of farmers (CK4, CK5). It can be seen that the composite microbial agent of the present application can replace urea and farmyard manure to play a corresponding role and is more convenient to use.

[0064] Example 5: A bacterial fermentation device for a composite microbial agent like Figure 1-13 As shown, a strain fermentation equipment of a composite microbial agent includes a fermentation tank 100, wherein a stirring device 200 is connected inside the fermentation tank 100, wherein the stirring device 200 is connected to a lifting device 300 connected to the fermentation tank 100, and the lifting device 300 is connected to a supporting culture device 400 connected to the fermentation tank 100; an air inlet pipe 500 is provided on the supporting culture device 400, and an air outlet device 600 is provided on the fermentation tank 100. In the strain fermentation equipment of a composite microbial agent of the present invention, when the strain is fermented and cultivated, the fermentation liquid can be sent into the fermentation tank 100 through the supporting culture device 400, and carried by the supporting culture device 400; a strain introduction port with a cover is provided on the top of the fermentation tank 100, so that the strain is easily introduced into the fermentation tank 100; an observation port is provided on the top of the fermentation tank 100, so that the fermentation state of the strain can be observed; during fermentation, the stirring device 200 can be started, and the stirring device 200 mixes and stirs the strain and the fermentation liquid, so as to improve the uniformity of strain dispersion and ensure the fermentation efficiency. When the stirring device 200 is mixing and stirring, it can also drive the lifting device 300 to operate. When the lifting device 300 is in operation, it can intermittently press the supporting culture device 400 upward, thereby controlling the supporting culture device 400 to drive the bacteria and the fermentation liquid to shake in the fermentation tank 100, thereby improving the mixing uniformity of the bacteria and the fermentation liquid. The supporting culture device 400 is provided with an air inlet pipe 500 to facilitate the injection of gas required for fermentation and to allow the gas to fully contact and mix with the fermentation liquid. The fermentation tank 100 is provided with a gas outlet device 600 to facilitate the discharge of gas generated during fermentation.

[0065] The fermentation tank 100 includes a U-shaped tank body 101 and a tank cover 102 detachably connected to the top of the U-shaped tank body 101. The arrangement of the tank cover 102 makes the U-shaped tank body 101 easy to clean or repair, and the two can be sealed and connected by bolts; a temperature control interlayer 103 is arranged inside the U-shaped tank body 101, and the temperature control interlayer 103 is connected to a temperature control inlet 104 and a temperature control outlet 105 fixed at both ends of the U-shaped tank body 101, so that media of different temperatures can be injected into the temperature control interlayer 103 through the temperature control inlet 104 to adjust the temperature inside the fermentation tank 100, and the medium can be discharged through the temperature control outlet 105.

[0066] The temperature control inlet 104 and the temperature control outlet 105 at both ends of the U-shaped tank body 101 are respectively rotatably connected to the two side plates of the equipment bracket 700, and the two ends of the bottom of the U-shaped tank body 101 are respectively connected to the bottom plate of the equipment bracket 700 through a supporting spring body 800, so as to reduce the vibration generated by the internal stirring device 200 of the present invention during operation, play a role of shock absorption and buffering to a certain extent, and facilitate the shaking of the U-shaped tank body 101. A discharge outlet is provided at the bottom of one side of the U-shaped tank body 101, which is convenient for discharging fermentation liquid or waste water during cleaning, and the discharge effect can be improved by controlling the tilt of the U-shaped tank body 101.

[0067] The stirring device 200 includes a motor 201, which is installed on the fermentation tank 100. The motor 201 is connected to a drive shaft 202 that is rotated in the fermentation tank 100. The drive shaft 202 penetrates into the shaft body of the fermentation tank 100 and is installed with one or more flipping blades 203. After the motor 201 is started, the drive shaft 202 is driven to rotate. When the drive shaft 202 rotates, it can drive one or more flipping blades 203 to rotate in the fermentation tank 100, thereby mixing the bacteria and fermentation liquid in the fermentation tank 100; the drive shaft 202 penetrates out of the fermentation tank 100 and is keyed to the drive wheel 204 on the shaft body outside the fermentation tank 100, thereby driving the drive wheel 204 to rotate when rotating. The drive wheel 204 is connected to the input wheel 301 in the jacking device 300 through a drive belt, thereby driving the input wheel 301 to rotate, and the input wheel 301 is keyed to the worm gear 206. The worm 302 is mounted on the force-bearing bracket 303, so that when it rotates, it drives the worm 302 to rotate. The worm 302 rotated on the force-bearing bracket 303 is meshed with the worm wheel 305 fixed on the output shaft 304, so that when the worm 302 rotates, the meshing worm wheel 305 rotates. The output shaft 304 is rotatably connected to the fermentation tank 100 through the force-bearing bracket 303. When the worm wheel 305 rotates, the output shaft 304 is synchronously driven to rotate. The output shaft 304 is fixed in the eccentric hole of the eccentric lifting wheel 306. The eccentric lifting wheel 306 is driven to connect the load-bearing culture device 400. When the output shaft 304 rotates, the eccentric lifting wheel 306 can be driven to rotate. Since the output shaft 304 is connected to the eccentric part of the eccentric lifting wheel 306, when the eccentric lifting wheel 306 rotates with the axis of the output shaft 304 as the center, it can be pressed to drive the load-bearing culture device 400 to move upward.

[0068] The load-bearing culture device 400 includes a load-bearing gear 401 fixed on a force-bearing transverse axis 402, and the lower end of the load-bearing gear 401 is meshed with a driving wheel 204; the force-bearing transverse axis 402 is connected to two linkage frames 403, and the two linkage frames 403 are connected to the upper ends of two lifting vertical plates 404, and the middle parts of the two lifting vertical plates 404 are sealed and slid on the fermentation tank 100, and the lower ends of the two lifting vertical plates 404 are inserted into the fermentation tank 100 and connected to a U-shaped load-bearing frame 405 arranged in a U-shaped groove inside the fermentation tank 100; the rectangular chamber arranged inside the lifting vertical plate 404 is connected to the U-shaped chamber arranged inside the U-shaped load-bearing frame 405, and the U-shaped chamber is connected to a plurality of medium ports 406 arranged on the U-shaped surface inside the U-shaped load-bearing frame 405, and ultrafiltration nets are arranged in the plurality of medium ports 406; the rectangular chamber of the lifting vertical plate 404 is connected to the air inlet pipe 500 which is threadedly sealed and connected to the outer side of the lifting vertical plate 404.

[0069] The outer wheel surface of the eccentric lifting wheel 306 is an outer gear ring structure, so that it can mesh with the load-bearing gear 401 to rotate. When the eccentric lifting wheel 306 rotates with the axis of the output shaft 304 as the center, since the output shaft 304 is connected to the eccentric part of the eccentric lifting wheel 306, when the wheel surface of the eccentric lifting wheel 306 farther from the output shaft 304 contacts the load-bearing gear 401, the load-bearing gear 401 can be driven to move upward by pressing. When the load-bearing gear 401 moves upward, the cooperation of the force-bearing horizontal axis 402 and the two linkage frames 403 can drive the two lifting vertical plates 404 to slide sealed on the fermentation tank 100, and the U-shaped load-bearing frame 405 can be driven up and down in the fermentation tank 100 through the two lifting vertical plates 404. The outer side surface of the support frame 405 is attached to the inner side surface of the fermentation tank 100 to ensure that it can drive the bacteria and fermentation liquid to move up and down, thereby shaking; when the gas used for bacteria fermentation is fed into the air inlet pipe 500, the gas can enter the rectangular chamber of the lifting vertical plate 404 through the air inlet pipe 500, and enter the U-shaped chamber of the U-shaped support frame 405 through the rectangular chamber of the lifting vertical plate 404, and finally output through the multiple medium ports 406 on the U-shaped surface inside the U-shaped support frame 405. At this time, while feeding the gas, the gas flow can turn over the bacteria and fermentation liquid carried on the U-shaped support frame 405, thereby improving the mixing effect of the bacteria and the fermentation liquid while preventing the bacteria from settling, thereby ensuring the fermentation effect.

[0070] The carrying and cultivating device 400 also includes a two-way water pump 407, and the two ends of the two-way water pump 407 are respectively fixedly connected to a horizontal pipeline 408 and a vertical pipeline 409, the horizontal pipeline 408 is fixed on the inner side of the lifting vertical plate 404, and is connected to the rectangular chamber of the lifting vertical plate 404; the middle part of the vertical pipeline 409 is sealed and slid on the fermentation tank 100, and the lower end of the vertical pipeline 409 is inserted into the fermentation tank 100. The carrying and cultivating device 400 can not only be used for carrying and assisting air intake, but also can be used to control the circulation flow of fermentation liquid. At this time, the two-way water pump 407 is turned on in a first direction, and the two-way water pump 407 cooperates with the vertical pipeline 409 to draw in the fermentation liquid, and transports it to the rectangular chamber of the lifting vertical plate 404 through the horizontal pipeline 408, and can also fully contact with the gas sent in by the air inlet pipe 500. A one-way valve is provided on the air inlet pipe 500, so that the gas can only The gas and fermentation liquid are mixed and enter the rectangular chamber of the lifting vertical plate 404, and are output to the fermentation tank 100 through multiple medium ports 406 on the U-shaped surface inside the U-shaped support frame 405, so as to facilitate circulation; a drain pipe 410 is fixedly connected to the side of the longitudinal pipeline 409, and the outer end of the drain pipe 410 is threaded and sealed to connect the pipe plug, so as to facilitate the discharge of the fermentation liquid; a valve is provided on the longitudinal pipeline 409, and the valve is located below the drain pipe 410. When discharging the fermentation liquid, the valve on the longitudinal pipeline 409 is controlled to be closed, and at this time, the two-way water pump 407 is controlled to be opened in the second direction, so that the fermentation liquid in the fermentation tank 100 is pumped into the rectangular chamber of the lifting vertical plate 404 through multiple medium ports 406, and enters the upper part of the valve of the longitudinal pipeline 409 through the horizontal pipeline 408, so as to be discharged through the drain pipe 410 with the pipe plug removed and in an open state.

[0071] The gas outlet device 600 includes a gas outlet straight pipe 601, the middle part of the gas outlet straight pipe 601 is sealed and fixed in the exhaust hole at the top of the fermentation tank 100, the top of the gas outlet straight pipe 601 is sealed and slidably connected to a cylindrical sealing plug 602, the cylindrical sealing plug 602 is connected to a lifting vertical shaft 603, the lower end of the lifting vertical shaft 603 is fixed to a lifting disc 604, the lifting disc 604 is slidably connected to the middle part of a plurality of vertical sliding shafts 605, the top of the plurality of vertical sliding shafts 605 is fixedly connected to an annular seat 606, the annular seat 606 is connected to the inner top surface of the fermentation tank 100 by bolts, and is fixed to the lifting disc 604 and the annular seat Multiple air outlet pressure springs 607 between 606 are sleeved on multiple vertical sliding shafts 605; the top of the cylindrical sealing plug 602 is fixedly connected to the limit plate 608, and the lower surface of the limit plate 608 is in contact with the air outlet straight pipe 601; the first air hole 609 at the bottom of the cylindrical sealing plug 602 and the second air hole 610 on the outer side of the cylindrical sealing plug 602 are both connected to the air cavity inside the cylindrical sealing plug 602, and the sealing sleeve 611 coaxially fixedly connected on the lower surface of the limit plate 608 is sealingly slidably matched on the outer side of the air outlet straight pipe 601, and a plurality of third air holes 612 are provided on the side of the sealing sleeve 611. As the fermentation gas is continuously injected into the fermentation tank 100, when a large amount of gas is generated after fermentation in the fermentation tank 100, the gas enters into the gas outlet straight pipe 601 and generates a large pressure on the cylindrical sealing plug 602. The cylindrical sealing plug 602 slides upward and drives the lifting vertical shaft 603 to move upward. The upward movement of the lifting vertical shaft 603 drives the lifting disc 604 to slide on the multiple vertical sliding shafts 605 and compresses the multiple gas outlet pressure springs 607, so that the second air hole 610 on the side of the cylindrical sealing plug 602 gradually separates from the gas outlet straight pipe 601. The gas in the fermentation tank 100 can be output to the outside of the gas outlet straight pipe 601 through the first air hole 609, the air cavity inside the cylindrical sealing plug 602 and the second air hole 610, and is discharged through the multiple third air holes 612 on the side of the sealing sleeve 611. The setting of the sealing sleeve 611 improves the sealing performance when no air is exhausted.

[0072] It is understandable that those skilled in the art can make equivalent substitutions or changes based on the technical solution and concept of the present invention, and all these changes or substitutions should fall within the protection scope of the claims attached to the present invention.

Claims

1. A composite microbial agent, characterized in that: The composite microbial agent mainly comprises the following components: 70-80 parts of microbial liquid, 2-5 parts of microbial promoter, and 20-30 parts of organic and inorganic nutrients.

2. A composite microbial agent according to claim 1, characterized in that: The microbial bacterial liquid comprises: Bacillus subtilis, Paenibacillus jelly, nitrogen-fixing bacteria and powdery Pichia pastoris.

3. A composite microbial agent according to claim 1, characterized in that: The microbial promoter includes one or more of rare earth elements, proteases, amino acids and betaine.

4. A composite microbial agent according to claim 1, characterized in that: The organic and inorganic nutrients include: 5-10 parts of potassium dihydrogen phosphate, 2-7 parts of calcium chloride, 3-5 parts of zinc sulfate, 10-12 parts of humic acid, and 50-80 parts of water.

5. A method for preparing the composite microbial agent according to any one of claims 1 to 4, characterized in that: The preparation method of the composite microbial agent comprises the following steps: S1: Preparation of bacterial activation, preparation of microbial promoters, and preparation of organic and inorganic nutrients; S2: inoculating the activated strains into the strain fermentation equipment according to proportion for fermentation; S3: Add microbial promoters and organic and inorganic nutrients to obtain a composite microbial agent.

6. The preparation method according to claim 5, characterized in that: The bacterial strain fermentation equipment used in step S2 comprises a fermentation tank (100), wherein a stirring device (200) is connected inside the fermentation tank (100), the stirring device (200) is connected to a lifting device (300) connected to the fermentation tank (100), and the lifting device (300) is connected to a supporting culture device (400) connected to the fermentation tank (100); an air inlet pipe (500) is provided on the supporting culture device (400), and an air outlet device (600) is provided on the fermentation tank (100).

7. The preparation method according to claim 6, characterized in that: The fermentation tank (100) comprises a U-shaped tank body (101) and a tank cover (102) detachably connected to the top of the U-shaped tank body (101); a temperature control interlayer (103) is provided inside the U-shaped tank body (101), and the temperature control interlayer (103) is communicated with a temperature control inlet (104) and a temperature control outlet (105) fixedly provided at both ends of the U-shaped tank body (101).

8. The preparation method according to claim 7, characterized in that: The temperature adjustment inlet (104) and the temperature adjustment outlet (105) at both ends of the U-shaped tank body (101) are respectively rotatably connected to the two side plates of the equipment bracket (700), and the two ends of the bottom of the U-shaped tank body (101) are respectively connected to the bottom plate of the equipment bracket (700) via a supporting spring body (800).

9. Use of the composite microbial agent according to any one of claims 1 to 4 and / or the composite microbial agent prepared by the preparation method according to any one of claims 5 to 8 in promoting plant growth.

10. The use according to claim 9, characterized in that: The dosage of the composite microbial agent is 3-10 L / mu.

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