Composite biopolysaccharide magnetic microsphere preparation method

Abstract

The present invention relates to the preparation process of magnetic microballoon of composite polysaccharide. The magnetic microballoon with certain magnetic response property is prepared with tamarind gelatin or sweet wormwood gelatin and chitosan as material, ferroferric iron as magnetofluid, glutaraldehyde as cross-linking agent and o-aminobenzene sulfonic acid as aminating agent. The magnetic microballoon has surface functional radical of -OSO2(C6H4)N-C(CH2)3CHO. The magnetic microballoon of composite polysaccharide has less volume swelling, high acid tolerance and low cost.

Description

technical field

[0001] The invention relates to a preparation method of composite biological polysaccharide magnetic microspheres. Background technique

[0002] Magnetic polymer microspheres refer to polymer microspheres that contain magnetic metal or metal oxide ultrafine powder inside and have magnetic responsiveness. It is a new type of functional polymer material developed in the past two decades. Magnetic polymer microspheres can introduce a variety of reactive functional groups on the surface of the microspheres through chemical reactions such as copolymerization and surface modification, and have specific reactivity, and can bind enzymes, antibodies, cells and other biologically active substances through covalent bonds . Magnetic polymer microspheres have certain magnetic responsiveness, so they have good operability and production performance, and can be used as carriers of enzymes, antibodies, cells or other biological materials in different fields.

[0003] Comp...

Examples

Embodiment 1

[0034] A kind of tamarind gum composite magnetic microsphere preparation method of the present invention is as follows:

[0035] 1. Preparation of water-soluble magnetic fluid: Mix ferrous sulfate aqueous solution and ferric chloride aqueous solution, add polyethylene glycol with a molecular weight of 10,000 under the condition of pH>11, 50°C, raise the temperature to 80°C, and react for 30 minutes. Naturally cool to room temperature, neutralize to neutral with dilute hydrochloric acid, let stand, remove the supernatant, that is, the water-soluble magnetic fluid is prepared, and set aside;

[0036] Wherein, the molar concentration of ferrous sulfate aqueous solution is 1 mole / liter; The molar concentration of ferric chloride aqueous solution is 1 mole / liter; The volume ratio of ferrous sulfate aqueous solution and ferric chloride aqueous solution consumption is 1: 2; Molecular weight is The amount of 10000 polyethylene glycol added in the mixed aqueous solution of ferrous sulf...

Embodiment 2

[0055] A kind of tamarind gum composite magnetic microsphere preparation method of the present invention is as follows:

[0056] 1. Preparation of water-soluble magnetic fluid: Mix ferrous sulfate aqueous solution and ferric chloride aqueous solution, add polyethylene glycol with a molecular weight of 10,000 under the condition of pH>11 and 60°C, raise the temperature to 80°C, and react for 50 minutes , naturally cooled to room temperature, neutralized to neutral with dilute hydrochloric acid, allowed to stand, and removed the supernatant to obtain a water-soluble magnetic fluid for subsequent use;

[0057] Wherein, the molar concentration of ferrous sulfate aqueous solution is 1.25 mol / liter; The molar concentration of ferric chloride aqueous solution is 2 mol / liter; The volume ratio of ferrous sulfate aqueous solution and ferric chloride aqueous solution consumption is 1: 1.25; Molecular weight is The amount of polyethylene glycol of 10000 in the mixed aqueous solution of fe...

Embodiment 3

[0076] A kind of tamarind gum composite magnetic microsphere preparation method of the present invention is as follows:

[0077] 1. Preparation of water-soluble magnetic fluid: Mix ferrous sulfate aqueous solution and ferric chloride aqueous solution, add polyethylene glycol with a molecular weight of 10,000 under the condition of pH>11, 55°C, raise the temperature to 80°C, and react for 40 minutes. Naturally cool to room temperature, neutralize to neutral with dilute hydrochloric acid, let stand, remove the supernatant, that is, the water-soluble magnetic fluid is prepared, and set aside;

[0078] Wherein, the molar concentration of ferrous sulfate aqueous solution is 1.12 mol / liter; The molar concentration of ferric chloride aqueous solution is 1.5 mol / liter; The volume ratio of ferrous sulfate aqueous solution and ferric chloride aqueous solution consumption is 1: 1.5; Molecular weight is The amount of polyethylene glycol of 10000 in the mixed aqueous solution of ferrous su...

technical field

[0001] The invention relates to a preparation method of composite biological polysaccharide magnetic microspheres. Background technique

[0002] Magnetic polymer microspheres refer to polymer microspheres that contain magnetic metal or metal oxide ultrafine powder inside and have magnetic responsiveness. It is a new type of functional polymer material developed in the past two decades. Magnetic polymer microspheres can introduce a variety of reactive functional groups on the surface of the microspheres through chemical reactions such as copolymerization and surface modification, and have specific reactivity, and can bind enzymes, antibodies, cells and other biologically active substances through covalent bonds . Magnetic polymer microspheres have certain magnetic responsiveness, so they have good operability and production performance, and can be used as carriers of enzymes, antibodies, cells or other biological materials in different fields.

[0003] Comp...

Examples

Embodiment 1

[0034] A kind of tamarind gum composite magnetic microsphere preparation method of the present invention is as follows:

[0035] 1. Preparation of water-soluble magnetic fluid: Mix ferrous sulfate aqueous solution and ferric chloride aqueous solution, add polyethylene glycol with a molecular weight of 10,000 under the condition of pH>11, 50°C, raise the temperature to 80°C, and react for 30 minutes. Naturally cool to room temperature, neutralize to neutral with dilute hydrochloric acid, let stand, remove the supernatant, that is, the water-soluble magnetic fluid is prepared, and set aside;

[0036] Wherein, the molar concentration of ferrous sulfate aqueous solution is 1 mole / liter; The molar concentration of ferric chloride aqueous solution is 1 mole / liter; The volume ratio of ferrous sulfate aqueous solution and ferric chloride aqueous solution consumption is 1: 2; Molecular weight is The amount of 10000 polyethylene glycol added in the mixed aqueous solution of ferrous sulf...

Embodiment 2

[0055] A kind of tamarind gum composite magnetic microsphere preparation method of the present invention is as follows:

[0056] 1. Preparation of water-soluble magnetic fluid: Mix ferrous sulfate aqueous solution and ferric chloride aqueous solution, add polyethylene glycol with a molecular weight of 10,000 under the condition of pH>11 and 60°C, raise the temperature to 80°C, and react for 50 minutes , naturally cooled to room temperature, neutralized to neutral with dilute hydrochloric acid, allowed to stand, and removed the supernatant to obtain a water-soluble magnetic fluid for subsequent use;

[0057] Wherein, the molar concentration of ferrous sulfate aqueous solution is 1.25 mol / liter; The molar concentration of ferric chloride aqueous solution is 2 mol / liter; The volume ratio of ferrous sulfate aqueous solution and ferric chloride aqueous solution consumption is 1: 1.25; Molecular weight is The amount of polyethylene glycol of 10000 in the mixed aqueous solution of fe...

Embodiment 3

[0076] A kind of tamarind gum composite magnetic microsphere preparation method of the present invention is as follows:

[0077] 1. Preparation of water-soluble magnetic fluid: Mix ferrous sulfate aqueous solution and ferric chloride aqueous solution, add polyethylene glycol with a molecular weight of 10,000 under the condition of pH>11, 55°C, raise the temperature to 80°C, and react for 40 minutes. Naturally cool to room temperature, neutralize to neutral with dilute hydrochloric acid, let stand, remove the supernatant, that is, the water-soluble magnetic fluid is prepared, and set aside;

[0078] Wherein, the molar concentration of ferrous sulfate aqueous solution is 1.12 mol / liter; The molar concentration of ferric chloride aqueous solution is 1.5 mol / liter; The volume ratio of ferrous sulfate aqueous solution and ferric chloride aqueous solution consumption is 1: 1.5; Molecular weight is The amount of polyethylene glycol of 10000 in the mixed aqueous solution of ferrous su...

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