Automatic film washing device for Western blot experiment

Abstract

The utility model provides an automatic membrane washing device for Western blot experiment, which relates to the technical field of Western blot experiment, and comprises a shell, both sides of the interior of the shell are fixedly connected with placing plates, and one side of each placing plate is provided with a membrane washing plate. According to the device, the threaded rod rotates to drive the two moving blocks to move, the inclined faces of the moving blocks make contact with the rotating rod, so that the rotating rod, the connecting rod and the rotating pressing plate rotate in sequence, one end of the rotating pressing plate rotates to be matched with the containing base, the end of a film can be fixed, and positioning of the film is achieved; the membrane is prevented from being poured away along with the solution after being cleaned; the motor works to drive the eccentric wheel to rotate, and the eccentric wheel is eccentrically arranged, continuously makes contact with and is separated from the push block and is matched with the spring, so that the film washing plate can be driven to reciprocate, a film in cleaning liquid is shaken and cleaned, and the cleaning effect on the film is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein blotting experiments, in particular to an automatic membrane washing device for Western blot experiments. Background Art

[0002] Protein blotting (immunoblotting test) is also known as Western Blot. It is a protein detection technology that transfers the total protein of cells or tissues separated by electrophoresis from the gel to a solid support NC membrane (nitrocellulose membrane) or PVDF membrane (polyvinylidene fluoride membrane), and then uses a specific antibody to detect a specific antigen. It is now widely used in many aspects such as gene expression research at the protein level, antibody activity detection and early diagnosis of diseases. Washing the membrane is a step in the Western Blot experiment and needs to be performed through a membrane washing device.

[0003] In the prior art, such as publication number CN205988933U, an automated membrane washing device for Western blot experiments is disclosed, including a first box body, the upper end of the first box body is connected to the second box body, and a TBST cleaning liquid storage tank is provided in one side of the inner cavity of the first box body, and the TBST cleaning liquid storage tank is through-connected with one end of a liquid storage tank through a liquid inlet pipe, a first solenoid valve and a first water pump are provided on the liquid inlet pipe, the liquid storage tank is rotatably connected to the second box body through a cylinder, and a cam device is provided between the liquid storage tank and the second box body to position and control the inclination angle of the liquid storage tank, a motor is provided on the cam device, and the bottom of the motor is fixedly connected to the upper end surface of the second box body.

[0004] Although the membrane can be washed by a membrane washing device, it is necessary to add membrane washing liquid and pour out waste liquid continuously and regularly for multiple times during each membrane washing stage. Since the operation is frequent, the experimenter needs to be on standby all the time, and the experimenter cannot carry out other work during the waiting time, which affects the work efficiency and the progress of the experiment. In addition, when pouring out the waste liquid, the membrane is easily poured out along with the waste liquid, which causes the membrane to be contaminated. Therefore, we propose an automated membrane washing device for Western blot experiments. Utility Model Content

[0005] The utility model aims to solve the problem in the prior art that it is inconvenient to effectively position the membrane for membrane washing, which leads to the membrane being easily poured out when the solution is poured, and proposes an automatic membrane washing device for Western blot experiments.

[0006] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: it includes a shell, both sides of the interior of the shell are fixedly connected with a placing plate, one side of the placing plate is provided with a film washing plate, both ends of one side of the film washing plate are fixedly connected with a placing base, one end of the placing base is rotatably connected with a connecting rod, the outside of the connecting rod is fixedly connected with a rotating pressure plate, one end of the connecting rod passes through the placing base and is fixedly connected with a rotating rod, a torsion spring is provided between one side of the rotating rod and one side of the placing base, the torsion spring is sleeved on the outside of the connecting rod, and a moving block is provided at one end of the rotating rod.

[0007] Furthermore, both ends of one side of the membrane washing plate are fixedly connected to a placement base, one side of the placement base is rotatably connected to a threaded rod, and the outside of the threaded rod is threadedly connected to the moving block.

[0008] Furthermore, a guide rod is fixedly connected to one side of the placement base, and the outside of the guide rod is slidably connected to the moving block.

[0009] Furthermore, a motor is fixedly connected to the outer side of the shell, a connecting shaft is fixedly connected to the output end of the motor, an eccentric wheel is fixedly connected to one end of the connecting shaft, a push block is overlapped at one end of the eccentric wheel, and one end of the push block is fixedly connected to one side of the membrane washing plate.

[0010] Furthermore, both ends of one side of one of the placement plates are provided with sliding grooves, the interior of the sliding groove is slidably connected with a slider, and one side of the slider is fixedly connected to one side of the membrane washing plate.

[0011] Furthermore, a spring is provided between one end of the sliding block and one end of the sliding slot.

[0012] Furthermore, a top cover is provided on one side of the shell.

[0013] Furthermore, one side of the top cover is fixedly connected to a connecting pipe, and one side of the shell is fixedly connected to a liquid outlet pipe.

[0014] Compared with the prior art, the advantages and positive effects of the utility model are:

[0015] 1. In the utility model, the two moving blocks can be driven to move by rotating the threaded rod, and the inclined surface of the moving block contacts the rotating rod, so that the rotating rod, the connecting rod and the rotating pressure plate rotate in sequence, and one end of the rotating pressure plate rotates and cooperates with the placement base to fix the end of the strip skin, thereby realizing the positioning of the membrane and preventing the membrane from being poured out with the solution after cleaning.

[0016] 2. In the utility model, the eccentric wheel is driven to rotate by the motor. The eccentric wheel is eccentrically arranged, and is constantly in contact with and separated from the push block. It cooperates with the spring to drive the membrane washing plate to reciprocate, and the membrane in the cleaning liquid is shaken and cleaned, thereby improving the cleaning effect on the membrane. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The utility model provides a three-dimensional structural schematic diagram of an automatic membrane washing device for Western blot experiments;

[0018] Figure 2 The utility model provides a schematic diagram of the internal cross-sectional structure of a shell of an automated membrane washing device for Western blot experiments;

[0019] Figure 3 The utility model provides a schematic diagram of the connection structure of a membrane washing plate of an automated membrane washing device for Western blot experiments;

[0020] Figure 4 The utility model proposes an automatic membrane washing device for Western blot experiments Figure 3 A local enlarged structural schematic diagram;

[0021] Figure 5 The utility model provides a schematic diagram of the disassembly structure of a membrane washing plate of an automatic membrane washing device for Western blot experiments.

[0022] Legend: 1. Shell; 2. Membrane washing plate; 3. Eccentric wheel; 4. Top cover; 5. Connecting pipe; 6. Liquid outlet pipe; 7. Placement plate; 8. Rotating pressure plate; 9. Connecting shaft; 10. Motor; 11. Push block; 12. Threaded rod; 13. Guide rod; 14. Placement base; 15. Connecting rod; 16. Rotating rod; 17. Torsion spring; 18. Moving block; 19. Slide groove; 20. Spring; 21. Slider. DETAILED DESCRIPTION

[0023] In order to more clearly understand the above-mentioned purpose, features and advantages of the utility model, the utility model is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0024] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0025] Embodiment 1, as Figure 1-Figure 5As shown, the utility model provides an automated membrane washing device for Western blot experiments, comprising a shell 1, both sides of the interior of the shell 1 are fixedly connected with a placement plate 7, one side of the placement plate 7 is provided with a membrane washing plate 2, both ends of one side of the membrane washing plate 2 are fixedly connected with a placement base 14, one end of the placement base 14 is rotatably connected with a connecting rod 15, the outside of the connecting rod 15 is fixedly connected with a rotating pressing plate 8, one end of the connecting rod 15 passes through the placement base 14 and is fixedly connected with a rotating rod 16, a torsion spring 17 is provided between one side of the rotating rod 16 and one side of the placement base 14, the torsion spring 17 is sleeved on the outside of the connecting rod 15, and a moving block 18 is provided at one end of the rotating rod 16; both ends of one side of the membrane washing plate 2 are fixedly connected with the placement base 14, one side of the placement base 14 is rotatably connected with a threaded rod 12, and the outside of the threaded rod 12 is threadedly connected to the moving block 18; one side of the placement base 14 is fixedly connected with a guide rod 13, and the outside of the guide rod 13 is slidably connected to the moving block 18.

[0026] The effect achieved by the entire embodiment 1 is as follows: open the top cover 4, put the membrane that needs to be washed into the shell 1, place the end of the membrane on the placement base 14, rotate the threaded rod 12, and the threads at both ends of the threaded rod 12 are set in opposite directions, driving the two moving blocks 18 to move, and the inclined edge of the moving block 18 contacts the rotating rod 16, pressing the two rotating rods 16 to rotate, and the torsion spring 17 is deformed by force, and the connecting rod 15 and the rotating pressure plate 8 rotate accordingly, and one end of the rotating pressure plate 8 is tilted to fix the end of the membrane to prevent the membrane from moving, thereby positioning the membrane and preventing the membrane from being poured out with the solution after cleaning. When the membrane needs to be taken out, the threaded rod 12 is rotated in the opposite direction to drive the moving block 18 to move and separate from the rotating rod 16, and the torsion spring 17 in the deformed state drives the rotating rod 16 to rotate, so that the rotating pressure plate 8 moves to restore its original state, and the membrane can be loosened and taken out.

[0027] Embodiment 2, as Figure 1 , Figure 2 and Figure 5 As shown, the difference between this embodiment and embodiment 1 is that a motor 10 is fixedly connected to the outer side of the shell 1, a connecting shaft 9 is fixedly connected to the output end of the motor 10, one end of the connecting shaft 9 is fixedly connected to the eccentric wheel 3, one end of the eccentric wheel 3 is overlapped with a push block 11, and one end of the push block 11 is fixedly connected to one side of the membrane washing plate 2; one end of one of the placement plates 7 is provided with a slide groove 19 at both ends, a slider 21 is slidably connected inside the slide groove 19, and one side of the slider 21 is fixedly connected to one side of the membrane washing plate 2; a spring 20 is provided between one end of the slider 21 and one end of the slide groove 19; a top cover 4 is provided on one side of the shell 1; one side of the top cover 4 is fixedly connected to a connecting pipe 5, and one side of the shell 1 is fixedly connected to a liquid outlet pipe 6.

[0028] The effect achieved by the entire embodiment 2 is that the motor 10 drives the connecting shaft 9 and the eccentric wheel 3 to rotate in sequence, the eccentric end of the eccentric wheel 3 rotates to contact with the push block 11, pushes the push block 11 to move, and the film washing plate 2 moves accordingly, the slider 21 slides inside the slide groove 19, the spring 20 stretches, and then the eccentric wheel 3 rotates to separate from the push block 11, the spring 20 elastic force drives the slider 21 to slide, and the film washing plate 2 moves to the initial position, and the eccentric wheel 3 rotates to continuously contact and separate from the push block 11, which can drive the film washing plate 2 to reciprocate, and the film fixed on the film washing plate 2 is washed. Shaking cleaning can improve the membrane washing effect. By setting a connecting pipe 5 to connect with the cleaning liquid delivery equipment, the cleaning liquid can be delivered to the inside of the shell 1 to wash the membrane inside. After five minutes of cleaning, a liquid outlet pipe 6 is provided on the outside of the shell 1, and a valve is installed at one end of the liquid outlet pipe 6. The valve is controlled to open to deliver the cleaning liquid inside the shell 1. The cleaning liquid inside the shell 1 can be cleaned, and then new cleaning liquid is delivered into the shell 1 through the connecting pipe, and then the membrane is cleaned. Multiple automatic membrane washing of the membrane washing device can be realized to improve the membrane washing effect.

[0029] Working principle: first open the top cover 4, put the membrane that needs to be washed into the shell 1, place the end of the membrane on the placement base 14, rotate the threaded rod 12, drive the two moving blocks 18 to move, the inclined edge of the moving block 18 contacts the rotating rod 16, presses the two rotating rods 16 to rotate, the torsion spring 17 is deformed by force, and the connecting rod 15 and the rotating pressure plate 8 rotate accordingly. One end of the rotating pressure plate 8 is tilted to fix the end of the membrane to prevent the membrane from moving. One end of the connecting pipe 5 is connected to the external cleaning liquid delivery equipment to deliver the cleaning liquid to the inside of the shell 1. The motor 10 drives the connecting shaft 9 and the eccentric wheel 3 to rotate, and the eccentric end of the eccentric wheel 3 rotates to It contacts the push block 11, pushes the push block 11 to move, and the membrane washing plate 2 moves accordingly, the slider 21 slides inside the slide groove 19, the spring 20 stretches, and then the eccentric wheel 3 rotates to separate from the push block 11, the elastic force of the spring 20 drives the slider 21 to slide, and the membrane washing plate 2 moves to the initial position, and the eccentric wheel 3 rotates to push the membrane washing plate 2 to reciprocate, and the membrane fixed on the membrane washing plate 2 is shaken and cleaned. After cleaning is completed, the valve on the liquid outlet pipe 6 is opened to directly release the cleaning liquid. By repeatedly sending the cleaning liquid into the shell 1 and sending the cleaned cleaning liquid out of the liquid outlet pipe 6, multiple automatic membrane washing of the membrane washing device can be achieved, thereby improving the membrane washing efficiency.

[0030] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. An automated membrane washing device for Western blot experiments, comprising a housing (1), characterized in that: Both sides of the interior of the shell (1) are fixedly connected with a placement plate (7), one side of the placement plate (7) is provided with a film washing plate (2), both ends of one side of the film washing plate (2) are fixedly connected with a placement base (14), one end of the placement base (14) is rotatably connected with a connecting rod (15), the outside of the connecting rod (15) is fixedly connected with a rotating pressure plate (8), one end of the connecting rod (15) passes through the placement base (14) and is fixedly connected with a rotating rod (16), a torsion spring (17) is provided between one side of the rotating rod (16) and one side of the placement base (14), the torsion spring (17) is sleeved on the outside of the connecting rod (15), and one end of the rotating rod (16) is provided with a moving block (18).

2. The automatic membrane washing device for Western blot experiments according to claim 1, characterized in that: Both ends of one side of the membrane washing plate (2) are fixedly connected to a placement base (14), one side of the placement base (14) is rotatably connected to a threaded rod (12), and the outside of the threaded rod (12) is threadedly connected to a moving block (18).

3. The automatic membrane washing device for Western blot experiments according to claim 2, characterized in that: A guide rod (13) is fixedly connected to one side of the placement base (14), and the outside of the guide rod (13) is slidably connected to the moving block (18).

4. The automatic membrane washing device for Western blot experiments according to claim 3, characterized in that: A motor (10) is fixedly connected to the outer side of the shell (1), the output end of the motor (10) is fixedly connected to a connecting shaft (9), one end of the connecting shaft (9) is fixedly connected to an eccentric wheel (3), one end of the eccentric wheel (3) is overlapped with a push block (11), and one end of the push block (11) is fixedly connected to one side of the membrane washing plate (2).

5. The automatic membrane washing device for Western blot experiments according to claim 4, characterized in that: One side of one of the placement plates (7) is provided with a slide groove (19) at both ends, the interior of the slide groove (19) is slidably connected with a slider (21), and one side of the slider (21) is fixedly connected to one side of the membrane washing plate (2).

6. The automatic membrane washing device for Western blot experiments according to claim 5, characterized in that: A spring (20) is provided between one end of the slider (21) and one end of the slide groove (19).

7. The automatic membrane washing device for Western blot experiments according to claim 6, characterized in that: A top cover (4) is provided on one side of the housing (1).

8. The automatic membrane washing device for Western blot experiments according to claim 7, characterized in that: One side of the top cover (4) is fixedly connected to a connecting pipe (5), and one side of the shell (1) is fixedly connected to a liquid outlet pipe (6).

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