Dog brain tissue sampling and storing device

Abstract

The utility model discloses a dog brain tissue sampling and storing device, and mainly relates to the field of animal sample collection. Comprising a collection assembly and a storage assembly. The collection assembly comprises an operation tube and a collection tube, and the bottom end of the operation tube is in threaded connection with the top end of the collection tube; the storage assembly comprises a storage pipe and a sealing cover which are matched with each other, and the cover bottom of the sealing cover is provided with an insertion groove which is movably connected with the top end of the operation pipe in an inserted mode. The dog brain tissue sample collecting device has the advantages that the dog brain tissue sample collecting operation is simpler, and sanitation and safety of the operation link are improved.

Description

Technical Field

[0001] The utility model relates to the field of animal sample collection, in particular to a canine brain tissue sampling and storage device. Background Art

[0002] Rabies is a disease that requires close inspection and prevention and control, and its onset is irregular. Therefore, the collection of samples from high-risk animals and the testing of samples for rabies virus nucleic acid and rabies virus antigen are the key business contents of this work.

[0003] When collecting, it is most appropriate to use living tissue specimens or fresh tissue specimens from dead animals, using the pipette method recommended by the World Health Organization (WHO) and the World Organization for Animal Health (OIE). After the dead animal is processed according to the prescribed procedures, a straw-shaped tool is used to insert it from the foramen magnum to one side of the eye, and then the outer end of the pipette tool is manually blocked, the pipette is pulled out, and the end containing the brain tissue is cut off about 2-3 cm with scissors and sealed in a sample storage tube / nucleic acid storage tube, and then it can be sent for inspection. The process of this operation mainly involves manual operation of both ends of the pipette. During the entire collection and cutting process, the top pipette needs to be blocked by hand, and it is easy to cause the collected sample to leak and be contaminated due to loose fingers. At the same time, during the process of cutting the bottom end, the plastic straw is easy to splash and drop the sample outside due to the elasticity and brittleness of its thin plastic, making the controllability during the operation very poor and easily causing pollution to the surrounding environment. Utility Model Content

[0004] The utility model aims to provide a canine brain tissue sampling and storage device, which simplifies the sample collection operation of canine brain tissue and improves the sanitation and safety of the operation link.

[0005] In order to achieve the above-mentioned purpose, the utility model is realized through the following technical solutions:

[0006] A canine brain tissue sampling and preservation device, comprising a collection component and a preservation component;

[0007] The collection assembly includes an operating tube and a collection tube, and the bottom end of the operating tube is threadedly connected to the top end of the collection tube;

[0008] The storage component comprises a matching storage tube and a sealing cover, and the bottom of the sealing cover is provided with a slot movably plugged with the top of the operating tube.

[0009] An upper pipe opening is arranged at the top end of the operating pipe, the diameter of the upper pipe opening corresponds to the inner diameter of the slot, and a sealing pad is arranged at the bottom of the slot.

[0010] The bottom end of the collection tube is provided with a collection port, and the collection port is an inclined pipe port structure.

[0011] The top of the collection tube is provided with a tube sleeve integral therewith, the inner wall of the tube sleeve is provided with an internal thread, and the outer wall of the bottom end of the operation tube is provided with an external thread.

[0012] An annular seat is provided at the top of the preservation tube, and protrusions are symmetrically provided on the inner wall of the annular seat. The protrusions are arched, and their arc sides are connected and fixed to the inner wall of the annular seat. A vertical end face is provided on the inner side of the protrusion. The pipe sleeve is non-circular, and the pipe sleeve can be inserted between two protrusions and stopped circumferentially by the end faces.

[0013] The sleeve is polygonal, special-shaped or elliptical, and:

[0014] When the sleeve is elliptical, the short axis of the sleeve is adapted to the distance between the end faces of the projections;

[0015] When the sleeve is of a special shape, the sleeve has two parallel and symmetrical cut surfaces on its circumference, and the distance between the two cut surfaces is adapted to the distance between the end surfaces of the protrusions;

[0016] When the sleeve is a quadrilateral, the width of the sleeve is adapted to the spacing between the end faces of the projections.

[0017] A connecting portion located above the protrusion is provided on the upper portion of the annular seat, and the connecting portion is threadedly connected and matched with the sealing cover.

[0018] The outer wall of the sealing cover is provided with a wave texture.

[0019] Compared with the prior art, the beneficial effects of the present invention are:

[0020] Through this device, based on the cooperation of each independent component, the operation process of sampling brain tissue of dogs and cats can be optimized. The improvements mainly include the following two aspects:

[0021] On the one hand, the sealing cover cooperates with the operating tube and the storage tube respectively, so that after being inserted into the corresponding position, the sealing cover can be matched with the top of the operating tube, thereby replacing the fingertips to block the tube opening, so that there is no need to keep the hand in the same position and the same hand shape in subsequent operations, which greatly reduces the difficulty of operation.

[0022] On the other hand, the sampling process is completed through a detachable collection tube. After sampling, the brain tissue remains in the collection tube. The two can be separated by rotating the collection tube and the operating tube without the aid of and contamination of other tools. The rotational separation process is easier to control than cutting the plastic tube, which greatly reduces sample splashing and leakage and improves the safety and hygiene of the operation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of each component of the utility model.

[0024] Figure 2 It is a schematic diagram of the assembly of the components of the utility model (when the sample is transferred into the storage tube).

[0025] Figure 3 It is a schematic diagram of the storage tube of the utility model.

[0026] Figure 4 It is a schematic diagram of the utility model storage tube after being closed.

[0027] Figure 5 It is a schematic diagram of the utility model for disassembling the sampling tube by cooperating the operating tube with the storage tube.

[0028] Figure 6 It is a schematic diagram of the combination of the operating tube and the sampling tube of the utility model when transferring samples.

[0029] Figure 7 It is a schematic diagram of the combination of the operating tube and the sampling tube of the utility model when being inserted.

[0030] Numbers shown in the accompanying drawings:

[0031] 1. Operating tube; 2. Upper tube opening; 3. Lower tube opening; 4. Collection tube; 5. Collection opening; 6. Tube sleeve; 7. Cut surface; 8. Sealing cover; 9. Slot; 10. Storage tube; 11. Ring seat; 12. Step; 13. Bump; 14. End face; 15. Connecting part. DETAILED DESCRIPTION

[0032] The present invention is further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the present application.

[0033] The instruments, reagents, materials, etc. involved in the following embodiments, unless otherwise specified, are all conventional instruments, reagents, materials, etc. in the prior art and can be obtained through regular commercial channels. The experimental methods, detection methods, etc. involved in the following embodiments, unless otherwise specified, are all conventional experimental methods, detection methods, etc. in the prior art.

[0034] Example 1: A canine brain tissue sampling and storage device

[0035] Includes collection components and storage components.

[0036] 1. Collection components

[0037] Collection Component The collection component includes an operating tube 1 and a collection tube 4 extending in a straight column.

[0038] Operation tube 1: The operation tube 1 is provided with a cylindrical tube cavity penetrating in the same direction thereof, the top end of the operation tube 1 is provided with an upper tube opening 2, and the bottom end of the operation tube 1 is provided with a lower tube opening 3;

[0039] Collection tube 4: A collection port 5 is provided at the bottom end of the collection tube 4. The collection port 5 is an inclined tube port, so that the bottom end has a sharp structure for easy insertion.

[0040] A pipe sleeve 6 is provided at the top of the collection tube 4. The pipe sleeve 6 and the collection tube 4 are integrally formed by penetrating from top to bottom. An internal thread is provided on the inner wall of the pipe sleeve 6. The pipe sleeve 6 is threadedly connected to the bottom end of the operating tube 1. By relative rotation of the collection tube 4 and the operating tube 1, the top end of the collection tube 4 can be connected or separated from the bottom end of the operating tube 1.

[0041] Two symmetrical cut surfaces 7 are arranged on the outer wall of the pipe sleeve 6, and the two cut surfaces 7 are arranged in parallel.

[0042] The collection tube 4 is provided with a collection cavity running through it from top to bottom, and the top of the collection cavity is coaxially connected with the tube sleeve 6. By tightening the tube sleeve 6 on the bottom end of the operating tube 1, a connected tube cavity and collection cavity can be obtained, and the upper tube port 2 and the collection port 5 are connected.

[0043] 2. Save the component

[0044] The storage assembly comprises a storage tube 10 and a sealing cap 8 .

[0045] The outer wall of the sealing cover 8 is provided with a wave texture, which is non-slip and easy to rotate and hold. The bottom side of the sealing cover 8 is provided with a slot 9 that is movably plugged with the upper tube mouth 2. The inner diameter of the slot 9 corresponds to the outer diameter of the operating tube 1. By inserting the upper tube mouth 2, the upper tube mouth 2 is blocked. The bottom of the slot 9 is provided with a rubber sealing pad to improve the sealing performance of the blockage, thereby replacing the operation of blocking the top of the straw with the belly of the finger. During the entire operation, the fingers are released from the restriction of the top tube mouth, and there is no need to press the tube mouth. The operating tube 1 can be directly held to cooperate with the pull-out and movement, which is convenient for operating the operating tube 1 more safely and flexibly.

[0046] The storage tube 10 is a transparent plastic bottle tube structure with a closed bottom end, and a circular open structure is provided at the top of the storage tube 10 . The diameter of the storage tube 10 is 1.5-3 times the diameter of the collection tube 4 .

[0047] An annular seat 11 is provided at the top of the preservation tube 10. The annular seat 11 is a circular ring component coaxial with the preservation tube 10. A step 12 is provided on the inner ring of the annular seat 11. The inner ring of the annular seat 11 located below the step 12 corresponds to the outer diameter of the preservation tube 10 and is used for sleeve-fitting and sealing (can be bonded) and fixed to the top of the preservation tube 10. The top end edge of the preservation tube 10 abuts against the step 12, which is convenient for processing and assembly.

[0048] The inner wall of the annular seat 11 is symmetrically provided with protrusions 13, the protrusions 13 are arched, and the arc side thereof is connected and fixed to the inner wall of the annular seat 11, and a vertical end face 14 is provided on one side of the protrusion 13 close to the center of the annular seat 11, the end faces 14 of the two protrusions 13 are parallel, and the distance between the two end faces 14 is 2 mm larger than the distance between the two cut surfaces 7, so as to facilitate the insertion of the pipe sleeve 6.

[0049] The upper part of the annular seat 11 is provided with a connecting portion 15 located above the protrusion 13, which is used to cooperate with the sealing cover 8, and can be plugged in or threaded to achieve the sealing closure of the storage tube 10. At the same time, the operation of the protrusion 13 can be shielded to ensure that the tube sleeve is located in the annular seat 11 when the tube sleeve is rotated to loosen, further strengthening the protection of the sample and preventing leakage.

[0050] When the collection tube 4 is inserted until the tube sleeve 6 is located between the two protrusions 13, and the cut surface 7 of the tube sleeve 6 is facing the end surface 14 of the protrusion 13, when the operating tube 1 is rotated, the collection tube 4 cannot rotate under the restriction of the two protrusions 13, so that the threaded cooperation between the tube sleeve 6 and the bottom of the operating tube 1 is reversely loosened, so that the collection tube 4 at the bottom is separated from the operating tube 1 and falls into the storage tube 10. This operation method is simpler and safer than the existing operation of cutting the bottom with scissors. It does not need to use and contaminate other tools (scissors), and the sample will not splash or leak.

[0051] The operation method of this tool is:

[0052] The operation tube 1, the collection tube 4, the storage tube 10, and the sealing cap 8 are independent components. After removing them from the packaging, perform the following operations:

[0053] 1) First, tighten the lower pipe opening 3 of the operating pipe 1 (with external threads nearby that are clearly identifiable) with the pipe sleeve 6 at one end of the collection pipe 4 to connect the operating pipe 1 with the collection pipe 4;

[0054] 2) Holding the upper part of the operating tube 1, insert it into the treated head from the foramen magnum toward one eye;

[0055] 3) Align the slot 9 of the sealing cover 8 with the upper pipe opening 2 and insert it tightly to seal the top of the operating pipe 1 through the sealing cover 8;

[0056] 4) Pull out the operation tube 1. The top of the operation tube 1 is blocked by the sealing cap 8, so that the brain sample is retained in the collection tube 4.

[0057] 5) Control the operating tube 1 and insert the collection tube 4 at the bottom thereof into the storage tube 10 until the two cut surfaces 7 of the tube sleeve 6 are at the same height and in the same direction as the inner end surface 14 of the protrusion 13. Fix the storage tube 10 with one hand and rotate the operating tube 1 with the other hand to make the bottom end of the operating tube 1 and the tube sleeve 6 rotate relative to each other and disengage the threaded engagement. The collection tube 4 with the sample tissue falls into the storage tube 10;

[0058] 6) Remove the sealing cover 8 at the top of the operation tube 1, and tightly cover the sealing cover 8 with the annular seat 11 at the top of the storage tube 10, so that the storage tube 10 can seal and store the sample tissue.

[0059] Based on the above equipment and operation, the optimization of brain tissue sampling and preservation of dead dogs is achieved. The operation process is mainly improved in the following two aspects. First, the upper tube mouth 2 can be blocked by the cooperation of components, ensuring that the tube can be stably closed during subsequent extraction and movement, simplifying the operation. Second, the collection tube 4 and the operation tube 1 are designed to be detachable, and the collection tube 4 can be separated from the operation tube 1 through the annular seat 11 at the top of the preservation tube 10 without getting your hands wet, without the help of other tools, and compared with the cutting operation, it is less likely to leak sample tissue, greatly improving the hygiene and safety of the entire operation.

[0060] Example 2: A canine brain tissue sampling and storage device

[0061] Includes collection components and storage components.

[0062] The main difference from Example 1 lies in the pipe sleeve 6, which has a rectangular shape. The length of the pipe sleeve 6 is smaller than the inner diameter of the storage tube 10 and can be easily inserted into the annular seat 11. The width of the pipe sleeve 6 is adapted to the end face 14 on the inner side of the protrusion 13. Similarly, the end face 14 of the protrusion 13 can prevent the pipe sleeve 6 from rotating, so that when the operating tube 1 is rotated, the two can be separated.

Claims

1. A canine brain tissue sampling and storage device, characterized in that: Includes a collection component and a storage component; The collection assembly includes an operating tube and a collection tube, and the bottom end of the operating tube is threadedly connected to the top end of the collection tube; The storage component comprises a matching storage tube and a sealing cover, and the bottom of the sealing cover is provided with a slot movably plugged with the top of the operating tube.

2. A canine brain tissue sampling and storage device according to claim 1, characterized in that: An upper pipe opening is arranged at the top end of the operating pipe, the diameter of the upper pipe opening corresponds to the inner diameter of the slot, and a sealing pad is arranged at the bottom of the slot.

3. A canine brain tissue sampling and storage device according to claim 1, characterized in that: The bottom end of the collection tube is provided with a collection port, and the collection port is an inclined pipe port structure.

4. A canine brain tissue sampling and storage device according to claim 1, characterized in that: The top of the collection tube is provided with a tube sleeve integral therewith, the inner wall of the tube sleeve is provided with an internal thread, and the outer wall of the bottom end of the operation tube is provided with an external thread.

5. A canine brain tissue sampling and storage device according to claim 4, characterized in that: An annular seat is provided at the top of the preservation tube, and protrusions are symmetrically provided on the inner wall of the annular seat. The protrusions are arched, and their arc sides are connected and fixed to the inner wall of the annular seat. A vertical end face is provided on the inner side of the protrusion. The pipe sleeve is non-circular, and the pipe sleeve can be inserted between two protrusions and stopped circumferentially by the end faces.

6. A canine brain tissue sampling and storage device according to claim 5, characterized in that: The sleeve is polygonal, special-shaped or elliptical, and: When the sleeve is elliptical, the short axis of the sleeve is adapted to the distance between the end faces of the projections; When the sleeve is of a special shape, the sleeve has two parallel and symmetrical cut surfaces on its circumference, and the distance between the two cut surfaces is adapted to the distance between the end surfaces of the protrusions; When the sleeve is a quadrilateral, the width of the sleeve is adapted to the spacing between the end faces of the projections.

7. A canine brain tissue sampling and storage device according to claim 5, characterized in that: A connecting portion located above the protrusion is provided on the upper portion of the annular seat, and the connecting portion is threadedly connected and matched with the sealing cover.

8. A canine brain tissue sampling and storage device according to claim 1, characterized in that: The outer wall of the sealing cover is provided with a wave texture.

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