Cell culture fluid container
By introducing a servo motor and heating pad into the cell culture medium container, the problem of culture medium and cell deposition in the cell culture flask is solved, ensuring the full growth environment and temperature stability of the cells.
Patent Information
- Application Number
- CN202420738565.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-10
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2034-04-10
AI Technical Summary
In existing cell culture flasks, culture medium and cells are easily deposited at the bottom of the flask, which makes it inconvenient to maintain a sufficient growth environment.
A cell culture liquid container including a transparent bottle, a motor box, a rotating assembly and a thermal insulation assembly was designed to drive the agitator rod to avoid deposition by a servo motor, and maintain a constant temperature through a heating pad and a temperature controller.
A uniform nutrient solution distribution is achieved, cell deposition is avoided, the cells are fully grown, and adverse reactions caused by temperature changes are prevented.
Smart Images
Figure CN222139142U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture fluid container. Background Art
[0002] Cell culture technology is also called cell cloning technology. The formal term in biology is cell culture technology. Cell culture is an indispensable process for both the entire bioengineering technology and one of its biological cloning technologies. Cell culture itself is the large-scale cloning of cells. Cell culture includes both the culture of microbial cells and cell culture technology. A cell can be cultured in large quantities to become a simple single cell or a multi-cell with very little differentiation. Usually, a cell culture medium container is required for cell culture.
[0003] A cell culture bottle disclosed in publication number CN219449697U is suitable for all cells that need to be cultured in a culture bottle and has the advantages of good use effect, convenient use and high work efficiency. The design of the bottle cap ensures that the cell culture bottle will not introduce other bacteria and impurities to affect the living environment of the cells.
[0004] However, the cell culture bottle has the following disadvantages: when the cell culture bottle is in use, the culture fluid and the cultured cells are easily deposited to the bottom of the culture bottle, and the culture fluid and the cultured cells are deposited and gathered together, which is not convenient for the cells at the bottom of the culture bottle to maintain a sufficient growth environment. Utility Model Content
[0005] 1. Technical issues to be resolved
[0006] The technical problem solved by the utility model is to provide a cell culture medium container which is highly practical, can be easily operated and has a relatively simple structure, thereby solving the problem in the above background technology that it is not convenient for cells at the bottom of the culture bottle to maintain a sufficient growth environment.
[0007] (II) Technical solution
[0008] To achieve the above objectives, the utility model is implemented through the following technical solutions: a cell culture fluid container, comprising a transparent bottle, the top surface of the transparent bottle is clamped with a bottle cap through a clamping block, the top surface of the bottle cap is fixedly connected to a motor box, a cavity is opened inside the motor box, a rotating component is fixedly connected inside the cavity, a fixed cylinder is clamped on the bottom surface of the transparent bottle, an insulation component is fixedly connected to the inner bottom wall of the fixed cylinder, and a plurality of clamping components are fixedly connected to the inner side wall of the fixed cylinder.
[0009] As a further solution of the utility model, the rotating assembly includes a servo motor fixedly connected to the inside of the cavity, and the output end of the servo motor is spline-connected to a transmission rod, and the transmission rod is used to transmit power.
[0010] As a further solution of the utility model, one end of the transmission rod is threadedly connected to a stirring rod, and one side of the servo motor is electrically connected to a battery A through a power line, and battery A is used to power the servo motor.
[0011] As a further solution of the utility model, the heat preservation component includes a heating pad fixedly connected to the bottom wall of the fixed cylinder, and one side of the heating pad is electrically connected to a battery B through a power line, and the battery B is used to supply power to the heating pad.
[0012] As a further solution of the utility model, the other side of the heating pad is electrically connected to a temperature controller via a power cord, and a gasket is provided on the top surface of the heating pad, which is used to reduce the buffering force of placing the transparent bottle on the heating pad.
[0013] As a further solution of the utility model, an anti-skid pad is fixedly connected to the bottom surface of the fixed cylinder. The anti-skid pad is made of rubber and is used for anti-skid.
[0014] (III) Beneficial effects
[0015] The utility model provides a cell culture fluid container, which has the following beneficial effects:
[0016] 1. The cell culture liquid container, through the setting of the rotating component, when in use, connects the battery A, turns on the servo motor, and the servo motor rotates slowly. The servo motor drives the transmission rod, and the transmission rod drives the stirring rod to prevent cells, other particles and nutrient solution from being deposited at the bottom of the transparent bottle, thereby achieving the effect of uniform nutrient solution and maintaining a sufficient growth environment for the cells at the bottom.
[0017] 2. The cell culture fluid container is provided with a heat preservation component. When in use, the battery B is connected, the heating pad is turned on, the temperature controller is turned on, the temperature controller adjusts the heating pad to a suitable temperature, the heating pad heats the transparent bottle, and the temperature change is avoided to cause adverse reactions of the cells, so that the cell culture container can be taken out of the incubator and can be kept in a similar temperature environment. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0019] Figure 2 This is a schematic diagram of the transparent bottle and bottle cap structure of the utility model;
[0020] Figure 3 This is a schematic diagram of the structure of the rotating assembly of the utility model;
[0021] Figure 4 This is a schematic diagram of the structure of the thermal insulation component and the clamping component of the utility model.
[0022] In the figure: 1. transparent bottle; 2. bottle cap; 3. motor box; 4. rotating assembly; 401. servo motor; 402. transmission rod; 403. stirring rod; 404. battery A; 5. fixing cylinder; 6. insulation assembly; 601. heating pad; 602. battery B; 603. temperature controller; 604. gasket; 7. clamping assembly; 701. spring; 702. buffer pad; 703. pressure plate; 8. anti-slip pad; 9. sealing ring. DETAILED DESCRIPTION
[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0024] See also Figures 1 to 4 The utility model provides a technical solution: a cell culture liquid container, comprising a transparent bottle 1, a bottle cap 2 is clamped on the top surface of the transparent bottle 1 through a clamping block, a motor box 3 is fixedly connected to the top surface of the bottle cap 2, a cavity is opened inside the motor box 3, a rotating assembly 4 is fixedly connected inside the cavity, and through the setting of the rotating assembly 4, when in use, a battery A404 is connected, a servo motor 401 is turned on, the servo motor 401 rotates slowly, the servo motor 401 drives a transmission rod 402, and the transmission rod 402 drives a stirring rod 403 to prevent cells, other particles and nutrient solution from being deposited on the bottom of the transparent bottle 1, thereby achieving uniform nutrition. Liquid, to maintain the effect of sufficient growth environment for the bottom cells, the bottom surface of the transparent bottle 1 is clamped with a fixed cylinder 5, the inner bottom wall of the fixed cylinder 5 is fixedly connected with a heat preservation component 6, through the setting of the heat preservation component 6, when in use, connect the battery B602, turn on the heating pad 601, turn on the temperature controller 603, the temperature controller 603 adjusts the heating pad 601 to a suitable temperature, the heating pad 601 heats the transparent bottle 1, avoids the temperature change causing adverse reactions of the cells, so that the cell culture container can be taken out of the incubator and can be kept in a similar temperature environment, and the inner side wall of the fixed cylinder 5 is fixedly connected with a plurality of groups of clamping components 7;
[0025] See also Figure 3 The rotating assembly 4 includes a servo motor 401 fixedly connected to the inside of the cavity, and the output end of the servo motor 401 is spline-connected to a transmission rod 402. Through the setting of the rotating assembly 4, a rotating stirring function is achieved;
[0026] See also Figure 3One end of the transmission rod 402 is threadedly connected to a stirring rod 403, and one side of the servo motor 401 is electrically connected to a battery A404 through a power line. The stirring rod 403 is provided to achieve a stirring effect;
[0027] See also Figure 4 The heat preservation component 6 includes a heating pad 601 fixedly connected to the inner bottom wall of the fixed cylinder 5. One side of the heating pad 601 is electrically connected to a battery B602 through a power line. The heat preservation component 6 plays a role of heat preservation;
[0028] See also Figure 4 The other side of the heating pad 601 is electrically connected to a temperature controller 603 through a power line, and a gasket 604 is provided on the top surface of the heating pad 601. The heating pad 601 plays a heating role;
[0029] See also Figure 4 The bottom surface of the fixed tube 5 is fixedly connected with an anti-skid pad 8, and the material of the anti-skid pad 8 is rubber. The setting of the anti-skid pad 8 plays an anti-skid role.
[0030] Embodiment 1:
[0031] See also Figure 2 The inside of the bottle cap 2 is fixedly connected with a sealing ring 9, and a hole is opened on the top surface of the bottle cap 2, and the hole is adapted to the transmission rod 402. The setting of the sealing ring 9 achieves a sealing effect;
[0032] Embodiment 2:
[0033] See also Figure 4 Several groups of clamping components 7 include springs 701 fixedly connected to the inner wall of the fixed tube 5, a buffer pad 702 is clamped inside the spring 701, and one end of the spring 701 is fixedly connected to a pressing piece 703. Through the setting of the clamping component 7, the transparent bottle 1 is fixed.
[0034] In the utility model, the working steps of the device are as follows:
[0035] Step 1: When in use, connect the battery A404, turn on the servo motor 401, the servo motor 401 rotates slowly, the servo motor 401 drives the transmission rod 402, and the transmission rod 402 drives the stirring rod 403;
[0036] The second step: when in use, connect the battery B602, turn on the heating pad 601, turn on the temperature controller 603, the temperature controller 603 adjusts the heating pad 601 to a suitable temperature, and the heating pad 601 heats the transparent bottle 1.
[0037] It should be noted that the equipment structure and drawings of the utility model mainly describe the principle of the utility model. In terms of the technology of the design principle, the settings of the power mechanism, power supply system and control system of the device are not fully described. On the premise that the technical personnel in this field understand the principle of the above utility model, the details of the power mechanism, power supply system and control system can be clearly known. The control method of the application document is automatic control through a controller, and the control circuit of the controller can be realized by simple programming by the technical personnel in this field;
[0038] The standard parts used therein can all be purchased from the market and can be customized according to the instructions and drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the structures and principles of the components known to technical personnel in this field can be known by these technical personnel through technical manuals or through conventional experimental methods.
[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A cell culture fluid container, comprising a transparent bottle (1), characterized in that: The top surface of the transparent bottle (1) is clamped with a bottle cap (2) via a clamping block, the top surface of the bottle cap (2) is fixedly connected to a motor box (3), a cavity is provided inside the motor box (3), a rotating assembly (4) is fixedly connected inside the cavity, a fixing cylinder (5) is clamped with the bottom surface of the transparent bottle (1), a heat preservation assembly (6) is fixedly connected to the inner bottom wall of the fixing cylinder (5), and a plurality of clamping assemblies (7) are fixedly connected to the inner side wall of the fixing cylinder (5).
2. A cell culture medium container according to claim 1, characterized in that: The rotating assembly (4) comprises a servo motor (401) fixedly connected to the interior of the cavity, and the output end of the servo motor (401) is spline-connected to a transmission rod (402).
3. A cell culture fluid container according to claim 2, characterized in that: One end of the transmission rod (402) is threadedly connected to a stirring rod (403), and one side of the servo motor (401) is electrically connected to a storage battery A (404) via a power line.
4. A cell culture fluid container according to claim 1, characterized in that: The heat preservation component (6) comprises a heating pad (601) fixedly connected to the inner bottom wall of the fixed cylinder (5), and one side of the heating pad (601) is electrically connected to a storage battery B (602) via a power line.
5. A cell culture fluid container according to claim 4, characterized in that: The other side of the heating pad (601) is electrically connected to a temperature controller (603) via a power line, and a gasket (604) is provided on the top surface of the heating pad (601).
6. A cell culture fluid container according to claim 1, characterized in that: The bottom surface of the fixed cylinder (5) is fixedly connected with an anti-skid pad (8), and the material of the anti-skid pad (8) is rubber.