Cell culture bottle suitable for obtaining tissue cells

Abstract

The utility model provides a cell culture bottle suitable for obtaining tissue cells, which belongs to the technical field of tissue cell culture devices and is characterized in that a pressing plate is arranged in a bottle body, a first magnet is embedded in the pressing plate, slots are arranged on a bottom plate and a top plate, and the slots on the bottom plate are opposite to the slots on the top plate in position. A second magnet corresponding to the first magnet can be inserted into the slot; a plurality of bulges are uniformly arranged on the surface of the bottom plate; an arc baffle is arranged at the end, close to the bottle opening, of the bottom plate, and the convex face of the arc baffle faces the bottle opening. By arranging the pressing plate, more tissue blocks can be pressed and attached between the bottom plate and the pressing plate to grow in a wall-attached manner, so that the wall-attached growth of the tissue blocks is facilitated, and the cell migration and climbing-out efficiency is improved; and through the arrangement of the arc-shaped baffle plate, the impact effect of the culture solution on the tissue block is reduced during the solution changing operation, and the falling of the adherent tissue block is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue cell culture devices, in particular to a cell culture bottle suitable for obtaining tissue cells. Background Art

[0002] The statements in this section merely provide background technology related to the present invention and do not necessarily constitute prior art.

[0003] In vitro cultured cells are divided into two categories, adherent and suspended, according to their growth characteristics of adhering to the support. Most cultured cells are adherent. The primary culture of adherent cells mainly adopts tissue block culture method. For example, the traditional culture of adipose stem cells, umbilical cord mesenchymal stem cells, cardiomyocytes, fibroblasts, tumor cells, kidney cells, etc. all adopt the adherent tissue block culture method. In tissue adherent culture, the tissue is guaranteed to grow adherently, the cell growth cycle is short, and it is easy to crawl out of the cultured tissue block. After the tissue block is adhered to the wall and cultured for a period of time, the cells will slowly crawl out. Therefore, adhesion is critical.

[0004] The inventors have discovered that when using existing traditional cell culture bottles for tissue cell adherence culture, adding tissue blocks and culture medium together into the culture bottle has the following defects: first, the natural sedimentation adhesion method has a long adhesion time, and the number of adherent tissue blocks is small, resulting in less cell migration; second, low-density tissue blocks are easily floated on the upper layer of the culture medium due to their small size, and thus cannot grow adherently, resulting in slow cell growth and low efficiency of cell migration and crawling out; third, some adherent tissue blocks may also detach from the wall due to the shaking of the culture bottle during the fluid change operation, and the floating tissue blocks need to be centrifuged and collected and then planted back into the bottle, which increases the operation steps and may also cause contamination and affect the cell yield.

[0005] The Chinese utility model patent with the announcement number CN220597534U discloses a cell culture bottle for tissue block adherent culture, which is provided with a pressing net inside the bottle body, and the tissue is pressed to the bottom of the bottle by the gravity of the pressing net, so as to prevent the problem that the tissue block is unstable, easy to float, and easy to fall off during the liquid change operation. However, the pressing net may completely press the tissue block to the bottom of the bottle, causing it to grow slowly and affect the cells to crawl out, or the pressing net may be suspended in the culture medium and fail to play the pressing effect, and the tissue block is still suspended in the culture medium, and the tissue block will still float to the surface of the culture medium through the mesh of the pressing net. In addition, the pressing net is unstable in the bottle and may be displaced forward and backward, so that in the process of adding the tissue block culture medium, the culture medium will be distributed on both sides of the pressing net, and after the bottle body is placed normally, the tissue block may be located above the pressing net, so that the pressing net cannot play the pressing effect. Utility Model Content

[0006] The utility model aims to provide a cell culture bottle suitable for obtaining tissue cells, which is convenient for tissue block adherence culture, can reduce tissue block shedding, and is conducive to cell migration, so as to solve at least one technical problem existing in the above-mentioned background technology.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] The utility model provides a cell culture bottle suitable for obtaining tissue cells, comprising a bottle body, one end of the bottle body is an inclined bottle mouth, the bottle mouth is connected to a bottle cap, the bottle cap is provided with an air vent, and the air vent is provided with a filter membrane; the bottle body is a structure in which a bottle mouth, a bottom plate, a top plate, a bottle bottom, and two side plates are integrally formed; a pressing plate is provided inside the bottle body, and a first magnet is embedded inside the pressing plate; slots are provided on both the bottom plate and the top plate, and the positions of the slots on the bottom plate and the slots on the top plate are opposite; a second magnet corresponding to the first magnet can be inserted into the slot; a plurality of protrusions are evenly provided on the surface of the bottom plate; an arc-shaped baffle is provided on the end of the bottom plate close to the bottle mouth, and the convex surface of the arc-shaped baffle faces the bottle mouth.

[0009] Optionally, the shape of one end of the pressing plate matches the concave surface of the arc-shaped baffle, the length of the pressing plate is smaller than the distance between the arc-shaped baffle and the bottle bottom; and the width of the pressing plate is smaller than the distance between the two side plates.

[0010] Optionally, there are gaps between the two ends of the arc-shaped baffle and the side plates, through which the culture fluid can flow.

[0011] Optionally, the side plates and the bottle bottom are both provided with slide grooves, and the sides of the pressing plate are provided with ear plates matching the slide grooves.

[0012] Optionally, a through hole is provided on the pressing plate.

[0013] Optionally, the protrusion is made integrally with the base plate.

[0014] Optionally, the protrusion is in an arc shape.

[0015] Optionally, the pressing plate is made of polyethylene material.

[0016] Optionally, the bottle body is made of polyethylene material.

[0017] Optionally, the number of the slots on the top plate is 4, the number of the slots on the bottom plate is 4, and the number of the first magnets embedded in the pressure plate is 4, corresponding to the positions of the slots.

[0018] The utility model has the following beneficial effects: by setting a pressing plate, embedding a first magnet in the pressing plate, and cooperating with a second magnet inserted in a slot of the top plate, the pressing plate is adsorbed on one side of the top plate, and when adding tissue blocks and culture fluid, it is ensured that the tissue blocks are located on the same side of the pressing plate, that is, between the pressing plate and the bottom plate, and when placed for culture, more tissue blocks can be pressed and attached to the wall between the bottom plate and the pressing plate for growth; after adding culture fluid, the second magnet in the slot of the top plate is pulled out and inserted into the slot of the bottom plate, and the pressing plate is adsorbed on the bottom plate by the second magnet, and in cooperation with the protrusions on the bottom plate, the tissue blocks are confined to the gaps between the protrusions, which is conducive to the wall-adhering growth of the tissue blocks and improves the efficiency of cell migration and crawling out; by setting an arc-shaped baffle, when performing a fluid replacement operation, the impact of the culture fluid on the tissue blocks is reduced, and the falling off of the wall-adhering tissue blocks is avoided.

[0019] Additional aspects and advantages of the present invention will be partially given in the following description, which will become apparent from the following description or be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is a three-dimensional structural diagram of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0022] Figure 2 This is a front view of the structure of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0023] Figure 3 This is a left-side structural diagram of a cell culture flask suitable for obtaining tissue cells described in an embodiment of the utility model.

[0024] Figure 4 This is a right view of the structure of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0025] Figure 5 This is a top view of the structure of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0026] Figure 6 This is a bottom view of the structure of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0027] Figure 7This is a structural diagram of the interior of a cell culture bottle suitable for obtaining tissue cells described in an embodiment of the utility model from a first viewing angle.

[0028] Figure 8 This is a structural diagram of the interior of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model, viewed from a second viewing angle.

[0029] Fig. 9 This is a cross-sectional view of the internal structure of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0030] Fig.10 This is a longitudinal structural cross-sectional view of a cell culture bottle suitable for obtaining tissue cells according to an embodiment of the utility model.

[0031] Among them: 1-bottle body; 2-bottle mouth; 3-bottle cap; 4-vent; 5-bottom plate; 6-top plate; 7-bottom of bottle; 8-side plate; 9-pressing plate; 10-slot; 11-second magnet; 12-protrusion; 13-arc baffle; 14-gap; 15-slide; 16-ear plate; 17-through hole; 18-guiding surface. DETAILED DESCRIPTION

[0032] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below by the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be interpreted as limiting the present invention.

[0033] Those skilled in the art will understand that, unless otherwise defined, all terms (including technical terms and scientific terms) used herein have the same meaning as generally understood by those skilled in the art to which the present invention belongs.

[0034] It should also be understood that terms, such as those defined in commonly used dictionaries, should be understood to have a meaning consistent with that in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless as defined herein.

[0035] Those skilled in the art will appreciate that, unless otherwise stated, the singular forms "a", "an", "said" and "the" used herein may also include plural forms. It should be further understood that the term "comprising" used in the specification of the present utility model refers to the presence of the features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements and / or groups thereof.

[0036] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples, unless they are contradictory.

[0037] In the description of this specification, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this utility model, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0038] In the description of this specification, the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present technology and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present technology.

[0039] Unless otherwise clearly specified or limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, they can be fixedly connected or set, or detachably connected or set, or integrally connected or set. For ordinary technicians in this field, the specific meanings of the above terms in this technology can be understood according to specific circumstances.

[0040] To facilitate the understanding of the present invention, the present invention is further explained below with reference to specific embodiments in conjunction with the accompanying drawings, and the specific embodiments do not constitute a limitation on the embodiments of the present invention.

[0041] Those skilled in the art should understand that the drawings are merely schematic diagrams of embodiments, and the components in the drawings are not necessarily necessary for implementing the present invention.

[0042] like Figures 1 to 9As shown, an embodiment of the utility model provides a cell culture bottle suitable for obtaining tissue cells, including a bottle body 1, one end of the bottle body 1 is an inclined bottle mouth 2, the bottle mouth 2 is connected to a bottle cap 3, a vent 4 is provided on the bottle cap 3, and a filter membrane is provided in the vent 4. The inclined bottle mouth 2 can be conveniently added with culture fluid, and it is convenient to insert a straw to absorb nutrient solution, which is convenient for liquid replacement operation. The bottle mouth 2 is provided with an external thread, and the bottle cap 3 is provided with an internal thread matching the external thread. The bottle mouth 2 and the bottle cap 3 are connected by threads. When the culture fluid and tissue blocks are added and cultured, the vent 4 on the bottle cap 3 can keep sufficient air in the bottle, and the filter membrane can filter impurities in the air into the bottle to prevent pollution.

[0043] The bottle body 1 is composed of a bottom plate 5, a top plate 6, a bottle bottom 7 and two left and right side plates 8. One end of the side plate 8 is inclined and arranged to be close to the middle and connected to the bottle mouth 2, so that the space near the bottle mouth 2 becomes smaller. At the same time, the position of the bottle mouth 2 is closer to the top plate 6. An inclined guide surface 18 is arranged between the lower edge of the bottle mouth 2 and the bottom plate 5 to ensure that during the process of adding culture solution, the culture solution can slowly flow into the bottle along the inclined guide surface 18. At the same time, one end of the side plate 8 is inclined and approaches the middle to reduce the space near the bottle mouth 2, so that the culture solution slowly expands in the bottle along the inclined part of the side plate 8. Combined with the effect of the guide surface 18, splashing of the culture solution is prevented and the degree of agitation of the culture solution during the addition process is reduced.

[0044] In the production of the bottle body 1, the bottom plate 5, the top plate 6, the bottle bottom 7 and the two side plates 8 can be connected by bonding to form the bottle body 1. Alternatively, the bottle body 1 is formed by the bottle mouth 2, the bottom plate 5, the top plate 6, the bottle bottom 7 and the two side plates 8 in one piece. The bottom plate 5, the top plate 6, the bottle bottom 7 and the two side plates 8 can be made of acrylic board or polyethylene material. In practical applications, the materials for making the bottle mouth 2, the bottom plate 5, the top plate 6, the bottle bottom 7 and the two side plates 8 are not limited by the acrylic board material and the polyethylene material, and those skilled in the art can select a suitable bottle body making material according to actual conditions.

[0045] In order to prevent the tissue block from floating on the surface of the culture solution or suspending, a pressing plate 9 is arranged inside the bottle body 1, and the tissue block in the culture solution is pressed into the culture solution through the pressing plate 9 and attached to the bottom plate 5 to prevent it from floating on the surface of the culture solution or suspending in the culture solution and not being able to grow on the wall. In the process of adding the culture solution, in order to prevent the culture solution from being added to both sides of the pressing plate 9, a first magnet is embedded inside the pressing plate 9, and at the same time, a second magnet 11 corresponding to the first magnet is arranged on the top plate 6. Through the attraction of the first magnet and the second magnet 11, the pressing plate 9 is adsorbed on the top plate 6, so that when the culture solution is added, it can be ensured that the culture solution is added on the same side of the pressing plate 9. A slot 10 is arranged on the top plate 6, and the second magnet 11 can be movably inserted into the slot 10. After the culture solution is added, the second magnet 11 in the slot 10 on the top plate 6 is pulled out, and the pressing plate 9 descends under the action of gravity to press the tissue block on the bottom plate 5. In order to prevent the pressing plate 9 from completely pressing the tissue block on the bottom plate 5 and destroying its growth, a protrusion 12 is provided on the bottom plate 5, and the protrusion 12 can be made integrally with the bottom plate 5. The protrusion 12 can separate the pressing plate 9 from the bottom plate 5, and the tissue block can fall into the space between the pressing plate 9 and the bottom plate 5. At the same time, the protrusion 12 can separate the tissue block, and the tissue block is attached to the space between several adjacent protrusions 12, or to the protrusion 12 or the bottom plate 5. By providing the pressing plate 9 and the protrusion 12, the adhesion rate of the tissue block is improved, and the efficiency and quality of cell climbing out are improved. Since the pressing plate 9 descends in the culture solution due to gravity, it may descend slowly and require a long waiting time. A slot 10 is also provided on the outer surface of the bottom plate 5, into which a second magnet 11 can be movably inserted. When the culture solution is added, the second magnet 11 in the slot 10 on the top plate 6 is pulled out and inserted into the slot 10 on the bottom plate 5. Under the action of suction, the pressing plate 9 descends faster. At the same time, a through hole 17 is provided on the pressing plate 9 to ensure that the culture solution can flow to the top of the pressing plate 9 through the through hole 17 during the descent of the pressing plate 9.

[0046] In a specific embodiment, the number of the first magnets on the pressing plate 9 is 4, all of which are embedded in the pressing plate 9 and located at the four corners of the pressing plate 9. Correspondingly, the positions of the slots 10 on the bottom plate 5 and the top plate 6 correspond to the positions of the first magnets on the pressing plate 9, and the number of the slots 10 also corresponds.

[0047] In actual applications, the number of first magnets is not limited to the above four. Those skilled in the art can set a suitable number according to actual conditions. Correspondingly, the setting position is not limited to the four corners. For example, one first magnet can be set at the middle of the pressing plate 9, two first magnets can be set at the two ends of the pressing plate 9, or three, five or more first magnets can be set. Correspondingly, the number and position of the slots 10 on the bottom plate 5 and the top plate 6 preferably correspond to the number and position of the first magnets. When the culture medium is added to the bottle, the second magnets 11 are inserted into the slots 10 on the top plate 6. When the culture medium is added and the tissue culture is performed, the second magnets 11 on the top plate 6 are pulled out and inserted into the slots 10 on the bottom plate 5.

[0048] In order to prevent the tissue blocks attached to the bottom plate 5 from being washed away and falling off when the culture medium is added during the liquid replacement process, an arc-shaped baffle 13 is provided at one end of the bottom plate 5 close to the bottle mouth 2, and the convex surface of the arc-shaped baffle 13 faces the direction of the bottle mouth 2. There are gaps 14 between the two ends of the arc-shaped baffle 13 and the two side plates 8. When new culture medium is added during the liquid replacement operation, the culture medium is prevented from directly flowing into the middle area of ​​the bottle bottom 5 under the blocking effect of the arc-shaped baffle 13. The culture medium flows in from the gap 14 between the arc-shaped baffle 13 and the side plates 8, thereby avoiding the washing of the tissue blocks in the middle area of ​​the bottom plate 5 and preventing the tissue blocks attached to the wall from falling off.

[0049] like Fig.10 As shown, the side of the arc baffle 13 opposite to the bottle mouth is an inclined surface, that is, the thickness of the arc baffle 13 gradually narrows from top to bottom. At the same time, the bottom plate area between the arc baffle 13 and the guide surface 18 is an inclined surface, that is, the thickness of the bottom plate gradually decreases from the side close to the bottle mouth to the arc baffle side.

[0050] The shape of one end of the pressing plate 9 matches the concave surface of the arc-shaped baffle 13, the length of the pressing plate 9 is less than the distance between the arc-shaped baffle 13 and the bottle bottom 7, and the width of the pressing plate 9 is less than the distance between the two side plates 8. In this way, the pressing plate 9 is located between the arc-shaped baffle 13 and the bottle bottom 7, and the arc-shaped baffle 13 will not block the descent of the pressing plate 9. The area between the arc-shaped baffle 13 and the guide surface 18 may not be provided with a protrusion 12 to avoid blocking the culture fluid. At the same time, a slide groove 15 is provided on the side plate 8 and the bottle bottom 7, and an ear plate 16 matching the slide groove 15 is provided on the side of the pressing plate 9. The ear plate 16 can slide in the slide groove 15. Through the cooperation of the slide groove 15 and the ear plate 16, the stability of the pressing plate 9 during descent is prevented, and its shaking or rollover is prevented, so as to ensure the stable and reliable pressure attachment of the tissue block.

[0051] In a specific embodiment, the shape of the protrusions 12 on the bottom plate 5 is an arc, and the arrangement can be 4 or 5 or more protrusions 12 are arranged in a group, and multiple groups are evenly distributed on the bottom plate 5. In practical applications, the shape of the protrusions is not limited to the above arc, and those skilled in the art can select the shape of the protrusions according to actual conditions, such as cone, triangular prism, etc.

[0052] The pressing plate 9 is made of polyethylene material. In practical applications, the material of the pressing plate 9 is not limited to the above materials, and those skilled in the art can select a suitable material according to actual conditions.

[0053] Although the above describes the specific implementation methods of the utility model in conjunction with the accompanying drawings, it is not intended to limit the scope of protection of the utility model. Technical personnel in the relevant field should understand that on the basis of the technical solution disclosed in the utility model, various modifications or deformations that can be made by technical personnel in this field without creative work should be included in the scope of protection of the utility model.

Claims

1. A cell culture bottle suitable for obtaining tissue cells, comprising a bottle body (1), one end of the bottle body being an inclined bottle mouth (2), a bottle cap (3) being connected to the bottle mouth (2), a vent hole (4) being provided on the bottle cap (3), and a filter membrane being provided in the vent hole (4); the bottle body (1) is an integrally formed structure of a bottle mouth (2), a bottom plate (5), a top plate (6), a bottle bottom (7), and two side plates (8); the characteristics are: A pressing plate (9) is provided inside the bottle body (1), and a first magnet is embedded inside the pressing plate (9); a slot (10) is provided on both the bottom plate (5) and the top plate (6), and the slot (10) on the bottom plate (5) and the slot (10) on the top plate (6) are opposite to each other; a second magnet (11) corresponding to the first magnet can be inserted into the slot (10); a plurality of protrusions (12) are evenly provided on the surface of the bottom plate (5); an arc-shaped baffle (13) is provided at one end of the bottom plate (5) close to the bottle mouth (2), and the convex surface of the arc-shaped baffle (13) faces the bottle mouth (2); the shape of one end of the pressing plate (9) matches the concave surface of the arc-shaped baffle (13), and the length of the pressing plate (9) is less than the distance between the arc-shaped baffle (13) and the bottle bottom (7); and the width of the pressing plate (9) is less than the distance between the two side plates (8).

2. The cell culture flask suitable for obtaining tissue cells according to claim 1, characterized in that: There are gaps (14) between the two ends of the arc-shaped baffle (13) and the side plate (8), through which culture fluid can flow.

3. The cell culture flask suitable for obtaining tissue cells according to claim 1, characterized in that: The side plate (8) and the bottle bottom (7) are both provided with a slide groove (15), and the side edge of the pressing plate (9) is provided with an ear plate (16) matching the slide groove (15).

4. The cell culture flask suitable for obtaining tissue cells according to claim 1, characterized in that: The pressing plate (9) is provided with a through hole (17).

5. The cell culture flask suitable for obtaining tissue cells according to claim 1, characterized in that: The protrusion (12) and the bottom plate (5) are made integrally.

6. The cell culture flask suitable for obtaining tissue cells according to any one of claims 1 to 5, characterized in that: The protrusion (12) is in an arc shape.

7. The cell culture flask suitable for obtaining tissue cells according to any one of claims 1 to 5, characterized in that: The pressing plate (9) is made of polyethylene material.

8. The cell culture flask suitable for obtaining tissue cells according to any one of claims 1 to 5, characterized in that: The bottle body (1) is made of polyethylene material.

9. The cell culture flask suitable for obtaining tissue cells according to claim 1, characterized in that: The number of slots (10) on the top plate (6) is 4, and the number of slots (10) on the bottom plate (5) is 4.

10. The cell culture flask suitable for obtaining tissue cells according to claim 9, characterized in that: The number of the first magnets embedded in the pressing plate (9) is four, corresponding to the positions of the slots (10).

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