Stem cell supernatant cryopreservation device

Abstract

The utility model provides a stem cell supernatant cryopreservation device which comprises a cryopreservation box and a sampling mechanism, a control top cover is installed on the surface of the top of the cryopreservation box, a covering cover is connected to the surface of the outer side of the control top cover through a hinge, a rotating shaft is rotatably connected to the surface of the inner side of the cryopreservation box, the sampling mechanism is arranged on the surface of the outer side of the cryopreservation box, and the sampling mechanism is connected with the control top cover through a hinge. Through the arrangement of the sampling mechanism, in the using process, efficient and large-range sampling of supernate is achieved, a control top cover does not need to be opened, temperature fluctuation is reduced, the stability of the cryopreservation environment can be maintained, the principle that labor is saved and the distance is wasted is adopted for the mechanism, and the working efficiency is improved. According to the stem cell cryopreservation device, large-range sampling is achieved more easily, a more flexible and efficient sampling mode is provided for researchers, the operation process of the stem cell cryopreservation device is optimized, the sampling efficiency of samples is improved, and meanwhile the influence of temperature changes on the cell quality is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field related to supernatant cryopreservation, and in particular to a stem cell supernatant cryopreservation device. Background Art

[0002] Stem cell supernatant cryopreservation is a key biomedical technology that aims to preserve the supernatant produced during stem cell culture, which contains many bioactive molecules and cytokines. The main purpose of this cryopreservation technology is to preserve the activity and effectiveness of these bioactive substances for future research, treatment or production purposes. During the process of stem cell culture, cells release a series of biomolecules, such as growth factors, cytokines and exosomes, which regulate and affect the surrounding cells while maintaining their own functions. By freezing the supernatant of stem cell culture, researchers can use these bioactive substances in future experiments or treatments to expand the application of stem cells. In the field of stem cell therapy, the freezing process usually involves the addition of appropriate cryoprotectants, such as dimethyl sulfoxide (DMSO), to prevent the biological molecules in the supernatant from being damaged during the freezing process. After freezing, the supernatant samples are stored in a liquid nitrogen tank at an extremely low temperature to ensure their long-term stability. The freezing of stem cell supernatant provides a powerful resource for future stem cell research and treatment, and helps to deepen the understanding of biological processes and treatment mechanisms. However, during use, there may be problems such as limited sampling area and difficulty in adjusting the sampling position during sampling, which limits the comprehensive sampling of the supernatant. Opening the top cover will cause heat loss in the freezing environment, so there is a special need for a stem cell supernatant freezing device.

[0003] However, in the existing stem cell supernatant freezing devices, most of the traditional stem cell supernatant freezing devices usually need to open the top cover when sampling, which will cause heat loss in the freezing environment and cause temperature fluctuations, which is not conducive to maintaining a constant low-temperature environment. During the operation, it may be necessary to open the control top cover multiple times, which increases the difficulty of sampling and also increases the impact on the freezing environment. Utility Model Content

[0004] The purpose of the utility model is to provide a stem cell supernatant freezing device to solve the problem of the existing stem cell supernatant freezing device proposed in the above-mentioned background technology. During use, the traditional freezing device usually needs to open the top cover when sampling, which will cause heat loss in the freezing environment and cause temperature fluctuations, which is not conducive to maintaining a constant low-temperature environment. During operation, it may be necessary to open the control top cover multiple times, which increases the difficulty of sampling and also increases the problem of the impact on the freezing environment.

[0005] To achieve the above-mentioned purpose, the utility model provides a stem cell supernatant freezing device, including a freezing box and a sampling mechanism, a control top cover is installed on the top surface of the freezing box, a covering cover is hingedly connected to the outer surface of the control top cover, the inner surface of the freezing box is rotatably connected to a rotating shaft, the outer surface of the freezing box is provided with a sampling mechanism, and the outer surface of the rotating shaft is provided with a liquid level mechanism.

[0006] Preferably, the sampling mechanism comprises a sampling slot, a driven gear, a driving gear, a flywheel, a crank, a slider, a sampling pipette and a guide rail. The outer surface of the freezing box is provided with a sampling slot, the outer surface of the rotating shaft is fixedly connected to the driven gear, and the inner surface of the freezing box is rotatably connected to the driving gear. The bottom surface of the driving gear is fixedly connected to the flywheel, the bottom surface of the flywheel is hingedly connected to the crank, the other end of the crank is hingedly connected to the slider, the inner surface of the slider is snap-connected to the sampling pipette, and the outer surface of the slider is slidably connected to the guide rail.

[0007] Preferably, the sampling slot is covered by a cover, the driven gear is meshed with the driving gear, and the number of teeth of the driven gear is greater than the number of teeth of the driving gear.

[0008] Preferably, the sampling straw passes through the slider and the guide rail, and the guide rail is fixedly connected to the inner surface of the freezing box.

[0009] Preferably, the liquid level mechanism includes a threaded groove, a limit groove, a storage cylinder, a knob and a compression spring, a threaded groove is provided on the outer surface of the rotating shaft, a limit groove is provided on the outer surface of the rotating shaft, a storage cylinder is slidably connected to the outer surface of the rotating shaft, a knob is threadedly connected to the outer surface of the rotating shaft, and a compression spring is fixedly connected to the top surface of the knob.

[0010] Preferably, the storage tube is slidably connected to the limiting groove, and the knob is threadedly connected to the thread groove.

[0011] Preferably, the other end of the compression spring is fixedly connected to the storage cylinder.

[0012] Compared with the prior art, the utility model has the following beneficial effects: the stem cell supernatant freezing device, through the setting of the sampling mechanism, realizes efficient and large-scale sampling of the supernatant during use, without opening the control top cover, reducing temperature fluctuations, and helping to maintain the stability of the freezing environment. The mechanism adopts the principle of saving effort and distance, and more easily realizes large-scale sampling, providing researchers with a more flexible and efficient sampling method, optimizing the operating process of the stem cell freezing device, improving the efficiency of sample collection, and reducing the impact of temperature changes on cell quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a side view schematic diagram of the overall structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the structure of the control top cover and the covering cover cooperating with each other in the utility model;

[0015] Figure 3 This is a schematic diagram of the structure of the cooperation between the driven gear and the driving gear of the utility model;

[0016] Figure 4 This is a schematic diagram of the structure of the driving gear and the crank cooperating with each other in the utility model;

[0017] Figure 5 This is a schematic diagram of the structure of the slide block and the sampling pipette cooperating with each other in the utility model;

[0018] Figure 6 This is a schematic diagram of the structure of the storage tube and the compression spring cooperating with each other in the utility model;

[0019] Figure 7 This is a schematic diagram of the structure in which the rotating shaft and the storage cylinder cooperate with each other in the utility model.

[0020] In the figure: 1. cryostat; 2. control top cover; 3. cover cover; 4. rotating shaft; 5. sampling mechanism; 501. sampling slot; 502. driven gear; 503. driving gear; 504. flywheel; 505. crank; 506. slider; 507. sampling pipette; 508. guide rail; 6. liquid level mechanism; 601. threaded groove; 602. limit groove; 603. storage cylinder; 604. knob; 605. compression spring. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the implementation regulations described are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1-7The utility model provides a stem cell supernatant freezing device: it includes a freezing box 1 and a sampling mechanism 5, a control top cover 2 is installed on the top surface of the freezing box 1, the outer surface of the control top cover 2 is hingedly connected to a covering cover 3, the inner surface of the freezing box 1 is rotatably connected to a rotating shaft 4, the outer surface of the freezing box 1 is provided with a sampling mechanism 5, the outer surface of the rotating shaft 4 is provided with a liquid level mechanism 6, a freezing component is installed inside the freezing box 1 to maintain internal stability, it is set by the control top cover 2 and can be opened for interaction with the internal supernatant, and the covering cover 3 covers the surface of the sampling slot 501 to prevent temperature dissipation when sampling is not required.

[0023] Furthermore, the sampling mechanism 5 includes a sampling groove 501, a driven gear 502, a driving gear 503, a flywheel 504, a crank 505, a slider 506, a sampling pipette 507 and a guide rail 508. The sampling groove 501 is opened on the outer surface of the freezing box 1, the outer surface of the rotating shaft 4 is fixedly connected to the driven gear 502, and the inner surface of the freezing box 1 is rotatably connected to the driving gear 503. The bottom surface of the driving gear 503 is fixedly connected with a flywheel 504, the bottom surface of the flywheel 504 is hingedly connected with a crank 505, the other end of the crank 505 is hingedly connected with a slider 506, the inner surface of the slider 506 is snap-connected with a sampling pipette 507, the outer surface of the slider 506 is slidably connected with a guide rail 508, through the arrangement of the sampling slot 501, the driven gear 502, the driving gear 503, the flywheel 504, the crank 505, the slider 506, the sampling pipette 507 and the guide rail 508, during use, the sampling slot 501 is exposed after the cover 3 is opened, the sampling pipette 507 is passed through the sampling slot 501 and inserted into the slider 506, at this time, the supernatant in the storage cylinder 603 can be sampled through the sampling pipette 507, and after sampling the current area, the sampling pipette 507 can be pushed and pulled to remove the supernatant. The tube 507 moves the slider 506, and the slider 506 is limited in its moving path by the guide rail 508, and a crank slider mechanism is formed through the crank 505 to drive the flywheel 504 to rotate, and the flywheel 504 drives the driving gear 503 to rotate and drives it to rotate by meshing with the driven gear 502. Since the number of teeth of the driven gear 502 is greater than the number of teeth of the driving gear 503, it has the effect of saving effort and distance. The driving gear 503 rotates several circles and the driven gear 502 rotates one circle. In this way, the rotation angle of the driven gear 502 can be better controlled and the storage cylinder 603 can be driven to rotate through the bottom shaft 4. In this way, the sampling pipette 507 can have a larger sampling area in the storage cylinder 603 without opening the control top cover 2, so as to prevent the outer cover from being opened when sampling is needed at other positions, resulting in heat loss and reducing the freezing effect.

[0024] Furthermore, the sampling slot 501 is covered by the covering cover 3, the driven gear 502 is meshed with the driving gear 503, the number of teeth of the driven gear 502 is greater than the number of teeth of the driving gear 503, and the covering cover 3 covers the surface of the sampling slot 501 to prevent temperature dissipation when sampling is not needed. The large transmission ratio saves effort and distance.

[0025] Furthermore, the sampling pipette 507 passes through the slider 506 and the guide rail 508 , and the guide rail 508 is fixedly connected to the inner surface of the freezing box 1 , and the supernatant in the storage cylinder 603 is sampled through the sampling pipette 507 .

[0026] Furthermore, the liquid level mechanism 6 includes a threaded groove 601, a limiting groove 602, a storage cylinder 603, a knob 604 and a compression spring 605. The outer surface of the rotating shaft 4 is provided with a threaded groove 601, the outer surface of the rotating shaft 4 is provided with a limiting groove 602, the outer surface of the rotating shaft 4 is slidably connected with the storage cylinder 603, the outer surface of the rotating shaft 4 is threadedly connected with the knob 604, and the top surface of the knob 604 is fixedly connected with the compression spring 605. Through the arrangement of the threaded groove 601, the limiting groove 602, the storage cylinder 603, the knob 604 and the compression spring 605, during use, the rotating shaft 4 is respectively connected to the storage cylinder 603 and the knob 604 through the threaded groove 601 and the limiting groove 602, and the limiting groove 602 can prevent the storage cylinder 603 from being locked. The cylinder 603 rotates on the shaft 4 without affecting the up and down movement, so that the shaft 4 can be driven to rotate synchronously when it rotates. The position of the knob 604 can be locked and connected to the top storage cylinder 603 through the compression spring 605. When the supernatant in the storage cylinder 603 is less, the storage cylinder 603 squeezes the compression spring 605 less, and the storage cylinder 603 is in a higher position. When the supernatant inside the storage cylinder 603 increases, the bottom compression spring 605 will be compressed and in a lower position. In this way, no matter how much the supernatant capacity inside the storage cylinder 603 is, the topmost liquid level will always be maintained at the required position for easy removal or sampling. The knob 604 controls the height of the liquid level by moving on the shaft 4 and driving the compression spring 605 to move.

[0027] Furthermore, the storage tube 603 is slidably connected to the limiting groove 602 , the knob 604 is threadedly connected to the thread groove 601 , and the rotating shaft 4 is connected to the storage tube 603 and the knob 604 respectively through the thread groove 601 and the limiting groove 602 .

[0028] Furthermore, the other end of the compression spring 605 is fixedly connected to the storage cylinder 603 , and the storage cylinder 603 will always maintain the topmost liquid level at a desired position through the compression spring 605 .

[0029] Working principle: A stem cell supernatant freezing device, a freezing box 1 is installed with a freezing component to keep the interior stable, a sampling slot 501 is exposed after opening the cover 3, a sampling pipette 507 is passed through the sampling slot 501 and inserted into the slider 506, at this time, the supernatant in the storage cylinder 603 can be sampled through the sampling pipette 507, and the slider 506 can be moved by pushing and pulling the sampling pipette 507, the slider 506 is limited in its moving path by the guide rail 508, and a crank slider mechanism is formed by the crank 505 to drive the flywheel 504 to rotate, the flywheel 504 drives the driving gear 503 to rotate and drives it to rotate by meshing with the driven gear 502, and the bottom The rotating shaft 4 drives the storage cylinder 603 to rotate. The storage cylinder 603 has a larger sampling area. When the supernatant in the storage cylinder 603 is less, the storage cylinder 603 squeezes the compression spring 605 less, and the storage cylinder 603 is in a higher position. When the supernatant inside the storage cylinder 603 increases, the bottom compression spring 605 will be compressed and in a lower position. In this way, no matter how much the supernatant capacity inside the storage cylinder 603 is, the topmost liquid level will always be maintained at the required position for easy removal or sampling. The knob 604 controls the height of the liquid level by moving on the rotating shaft 4 and driving the compression spring 605 to move, thereby completing a stem cell supernatant freezing device.

[0030] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to the implementation rules without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell supernatant cryopreservation device, comprising a cryopreservation box (1) and a sampling mechanism (5), characterized in that: The top surface of the freezing box (1) is provided with a control top cover (2), the outer surface of the control top cover (2) is hingedly connected to a covering cover (3), the inner surface of the freezing box (1) is rotatably connected to a rotating shaft (4), the outer surface of the freezing box (1) is provided with a sampling mechanism (5), and the outer surface of the rotating shaft (4) is provided with a liquid level mechanism (6); The sampling mechanism (5) comprises a sampling groove (501), a driven gear (502), a driving gear (503), a flywheel (504), a crank (505), a slider (506), a sampling pipette (507) and a guide rail (508). The outer surface of the freezing box (1) is provided with a sampling groove (501), the outer surface of the rotating shaft (4) is fixedly connected to the driven gear (502), the inner surface of the freezing box (1) is rotatably connected to the driving gear (503), the bottom surface of the driving gear (503) is fixedly connected to the flywheel (504), the bottom surface of the flywheel (504) is hingedly connected to the crank (505), the other end of the crank (505) is hingedly connected to the slider (506), the inner surface of the slider (506) is snap-connected to the sampling pipette (507), and the outer surface of the slider (506) is slidably connected to the guide rail (508).

2. A stem cell supernatant freezing device according to claim 1, characterized in that: The sampling slot (501) is covered by a cover (3), the driven gear (502) is meshed with the driving gear (503), and the number of teeth of the driven gear (502) is greater than the number of teeth of the driving gear (503).

3. The stem cell supernatant cryopreservation device according to claim 1, characterized in that: The sampling pipette (507) passes through the slider (506) and the guide rail (508), and the guide rail (508) is fixedly connected to the inner surface of the freezing box (1).

4. The stem cell supernatant cryopreservation device according to claim 1, characterized in that: The liquid level mechanism (6) comprises a thread groove (601), a limit groove (602), a storage cylinder (603), a knob (604) and a compression spring (605); the outer surface of the rotating shaft (4) is provided with a thread groove (601); the outer surface of the rotating shaft (4) is provided with a limit groove (602); the outer surface of the rotating shaft (4) is slidably connected to the storage cylinder (603); the outer surface of the rotating shaft (4) is threadedly connected to the knob (604); and the top surface of the knob (604) is fixedly connected to the compression spring (605).

5. A stem cell supernatant freezing device according to claim 4, characterized in that: The storage tube (603) is slidably connected to the limiting groove (602), and the knob (604) is threadedly connected to the thread groove (601).

6. The stem cell supernatant cryopreservation device according to claim 4, characterized in that: The other end of the compression spring (605) is fixedly connected to the storage cylinder (603).

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