Bearing device and sequencing system

Abstract

The utility model discloses a bearing device and a sequencing system. The bearing device comprises a bearing table, a flow cell assembly, a fluid connection assembly and a movement mechanism, the bearing table comprises a table top, the flow cell assembly is detachably located on the table top, the fluid connection assembly is connected with the movement mechanism, and the movement mechanism drives the fluid connection assembly to move between a first position and a second position when working. When the fluid connection assembly is located at the first position, the fluid connection assembly and the flow cell assembly form fluid communication; when the fluid connection assembly is in the second position, communication between the fluid connection assembly and the flow cell assembly is disconnected. According to the bearing device, the fluid connection assembly is driven by the movement mechanism to move relative to the flow cell assembly, connection and disconnection between the fluid connection assembly and the flow cell assembly can be achieved, and the bearing device is simple in structure and convenient to operate.

Description

Technical Field

[0001] The utility model relates to the field of gene sequencing, and in particular to a carrier device and a sequencing system. Background Art

[0002] Gene sequencing technology refers to the technical means of obtaining DNA or RNA base sequences through detection. The current dominant sequencing technology is high-throughput sequencing technology. In sequencing platforms based on sequencing by synthesis to achieve high-throughput sequencing, such as ILLUMINA's Miseq, Nextseq, and Novasek sequencing platforms, the general process of gene sequencing includes fixing the nucleic acid sample to be tested on a flow cell in a manner such as hybridization; then using PCR amplification to form nucleic acid molecule clusters from the nucleic acid sample to be tested; then adding sequencing reagents, such as bases with fluorescent groups, polymerases, and primers, to the flow cell, and through the principle of base complementary pairing, the bases with fluorescent groups are combined with the bases on the nucleic acid sample to be tested; finally, the fluorescent group is excited to produce fluorescence through an optical imaging system and the fluorescence is collected to form an image, and base recognition is performed through the image, thereby realizing the base sequence determination of the nucleic acid sample to be tested.

[0003] To achieve the above process, how to establish a fluid connection to enable reagents to flow into the flow cell and / or block the reagents from flowing into the flow cell is a technical issue that deserves attention. Utility Model Content

[0004] The utility model provides a carrying device and a sequencing system.

[0005] The carrying device of the embodiment of the present application includes a carrying platform, a flow pool assembly, a fluid connection assembly and a moving mechanism. The carrying platform includes a table top, the flow pool assembly is detachably located on the table top, the fluid connection assembly is connected to the moving mechanism, and the moving mechanism drives the fluid connection assembly to move between a first position and a second position when working. When the fluid connection assembly is located at the first position, the fluid connection assembly forms a fluid connection with the flow pool assembly; when the fluid connection assembly is located at the second position, the connection between the fluid connection assembly and the flow pool assembly is disconnected.

[0006] In the carrying device of the embodiment of the present application, the fluid connection component is driven to move relative to the flow pool component by the motion mechanism, so that the connection and disconnection between the fluid connection component and the flow pool component can be achieved. The carrying device has a simple structure and is easy to operate.

[0007] In some embodiments, the flow pool assembly includes a flow pool, with a fluid inlet and a fluid outlet respectively arranged at both ends of the flow pool, and the fluid connection assembly includes a manifold arranged corresponding to the fluid inlet and the fluid outlet, and the fluid connection assembly and the flow pool assembly form fluid communication with the fluid inlet and the fluid outlet through the manifold.

[0008] In this way, by connecting the fluid inlet and the fluid outlet to the manifold, communication between the fluid connection component and the flow pool component can be achieved, facilitating fluid circulation.

[0009] In certain embodiments, the fluid connection assembly includes a support member, and the manifold is disposed on the support member.

[0010] In this way, the support member can drive the manifold to move between the first position and the second position, thereby achieving connection and disconnection between the manifold and the flow pool.

[0011] In certain embodiments, the fluid connection assembly includes an elastic member connected to the manifold, and the elastic member is used to keep the manifold in a tendency to move toward the flow pool.

[0012] In this way, the elastic member can provide an elastic force to buffer the impact force between the flow cell and the manifold. At the same time, the elastic member can provide an elastic force to drive the manifold and the flow cell to press tightly together to ensure the sealing performance between the flow cell and the manifold.

[0013] In some embodiments, the motion mechanism includes a cam and a rotating shaft, the cam is connected to the rotating shaft, the rotating shaft can drive the cam to rotate, and the cam is also connected to the support member. When the cam rotates, it drives the manifold on the support member to move to the first position or the second position.

[0014] In this way, the cam can be driven to rotate by rotating the rotating shaft, thereby driving the support member and the manifold to move, thereby achieving connection and disconnection between the manifold and the flow pool.

[0015] In certain embodiments, a limiting hole is provided on the support member, and a cam is provided in the limiting hole. The limiting hole cooperates with the cam, so that when the cam rotates, it can drive the manifold on the support member to move to the first position or the second position.

[0016] In this way, the cam is set in the limiting hole, which can limit the movement range of the support member, and further limit the movement range of the manifold, so that the manifold can move between the first position and the second position to achieve connection and disconnection between the manifold and the flow pool.

[0017] In some embodiments, the limiting hole is square and includes a first inner wall and a second inner wall. When the cam abuts against the first inner wall, the cam drives the manifold away from the flow pool; when the cam abuts against the second inner wall, the cam drives the manifold close to the flow pool.

[0018] In this way, by abutting the cam against the first inner wall and the second inner wall, the movement range of the support member can be limited, thereby limiting the movement range of the manifold, so that the manifold can move between the first position and the second position, thereby achieving connection and disconnection between the manifold and the flow pool.

[0019] In some embodiments, the limiting hole includes a third inner wall, which is arranged opposite to the first inner wall and closer to the manifold relative to the first inner wall. When the cam abuts against the third inner wall, the cam drives the manifold closer to the flow pool.

[0020] In this way, by abutting the cam against the third inner wall, the movement range of the support member can be limited, thereby limiting the movement range of the manifold, so that the manifold can move between the first position and the second position, thereby achieving connection and disconnection between the manifold and the flow pool.

[0021] In some embodiments, the carrying device includes a driving mechanism, and the driving mechanism is used to drive the rotating shaft to rotate.

[0022] In this way, the driving mechanism can drive the rotating shaft to rotate, thereby driving the cam to rotate, thereby driving the movement of the support member and the manifold, and realizing the connection and disconnection between the manifold and the flow pool.

[0023] In some embodiments, the driving mechanism includes a driving member and a transmission assembly, the transmission assembly connects the driving member and the rotating shaft, and the driving member is used to drive the rotating shaft to rotate through the transmission assembly.

[0024] In this way, the driving member drives the rotating shaft to rotate through the transmission assembly, thereby driving the cam to rotate, thereby driving the movement of the supporting member and the manifold, thereby achieving connection and disconnection between the manifold and the flow pool.

[0025] In some embodiments, the transmission assembly includes a first synchronous wheel, a second synchronous wheel and a synchronous belt, the first synchronous wheel is connected to the driving member, the second synchronous wheel is connected to the rotating shaft, and the synchronous belt is wound around the first synchronous wheel and the second synchronous wheel.

[0026] In this way, the rotating shaft can be rotated through the transmission assembly, thereby driving the cam to rotate relative to the flow pool, thereby driving the manifold to be connected to and disconnected from the flow pool fluid.

[0027] In some embodiments, the driving member is a manual driving member, including a rotating part and an operating part connected to the rotating part, and the rotating part is connected to the first synchronous wheel.

[0028] In this way, the rotation of the rotating part and the first synchronous wheel can be manually adjusted through the manual drive component, and the connection between the fluid connection component and the flow pool component can be manually controlled to achieve precise control of the fluid. The structure is simple, the operation is convenient, and the manufacturing and maintenance costs are low.

[0029] In some embodiments, the number of the carrying platform, the movement mechanism and the driving mechanism are all multiple and arranged in a one-to-one correspondence, and the driving members of the multiple driving mechanisms are arranged on the same side of the carrying device.

[0030] In this way, the number of the carrier, the motion mechanism and the driving mechanism are all multiple, and multiple flow cells can be fixed at the same time, which is convenient for sequencing the samples to be tested on multiple flow cells at the same time, thereby improving the sequencing efficiency. In addition, the driving parts of the multiple driving mechanisms are arranged on the same side of the carrier device, which can reduce the space occupied by the driving parts, making the structure of the carrier device compact, thereby improving the space utilization rate of the carrier device and reducing the volume of the carrier device.

[0031] In some embodiments, the bearing device includes a mounting platform, the bearing platform and the motion mechanism are respectively arranged on both sides of the mounting platform, and the support member is inserted into the mounting platform.

[0032] In this way, the mounting platform is connected and fixed to the carrier platform, the motion mechanism and the support member, which can improve the stability of the carrier device, and then improve the stability of the flow cell, thereby improving the sequencing accuracy.

[0033] In some embodiments, at least one first groove and at least one second groove are provided on the surface of the supporting platform, and the first groove is connected to the second groove.

[0034] In this way, by providing at least one first channel and at least one second channel in communication, a plurality of openings can be formed on the table surface for adsorbing the flow cell, thereby fixing the flow cell on the table surface.

[0035] In some embodiments, the first channel and the second channel are arranged to cross each other.

[0036] In this way, by arranging the first groove and the second groove in a cross manner, the coverage of the first groove and the second groove on the table surface can be improved, it can be suitable for flow cells of different sizes, and the adsorption effect of the carrier device can be improved.

[0037] In some embodiments, there are multiple first grooves, and the multiple first grooves are spaced apart and arranged in parallel, there are multiple second grooves, and the multiple second grooves are spaced apart and arranged in parallel, and the first grooves and the second grooves are perpendicular to each other.

[0038] In this way, multiple first grooves and multiple second grooves are arranged vertically by crossing each other, so that the first grooves and the second grooves can form openings in a grid layout on the table. When the flow pool is placed on the table, the flow pool can be adsorbed and fixed by generating negative pressure at the openings of the first grooves and the second grooves.

[0039] In certain embodiments, the first channel and / or the second channel are connected to an external vacuum system.

[0040] In this way, the first channel and / or the second channel can generate negative pressure at the opening on the table, tightly joining the flow pool to the table, with a good fixing effect, while improving the flatness of the flow pool and reducing damage to the flow pool.

[0041] The sequencing system of the embodiment of the present application includes a carrying device.

[0042] In this way, the sequencing system is convenient for detecting the flow cell on the carrier device, is easy to operate, has high sequencing efficiency, and has high detection accuracy.

[0043] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0045] Figure 1 It is a structural schematic diagram of a carrying device according to an embodiment of the utility model;

[0046] Figure 2 It is a structural schematic diagram of a carrying device according to an embodiment of the utility model;

[0047] Figure 3 It is a structural schematic diagram of a carrying device according to an embodiment of the utility model;

[0048] Figure 4 It is a structural schematic diagram of a carrying device according to an embodiment of the utility model;

[0049] Figure 5 It is a structural schematic diagram of a carrying device according to an embodiment of the utility model;

[0050] Figure 6 It is a schematic structural diagram of a flow cell assembly according to an embodiment of the utility model;

[0051] Figure 7 It is a structural schematic diagram of a manifold according to an embodiment of the utility model;

[0052] Figure 8 yes Figure 5 An enlarged schematic diagram of part I of the carrying device;

[0053] Fig. 9 It is a schematic structural diagram of a support member according to an embodiment of the utility model.

[0054] Explanation of the reference numerals: 100, carrying device; 10, carrying platform; 11, table top; 12, first groove; 13, second groove; 20, flow pool assembly; 21, flow pool; 22, fluid inlet; 23, fluid outlet; 30, fluid connection assembly; 31, manifold; 311, fluid channel; 32, support member; 33, mounting hole; 34, elastic member; 35, groove; 36, limit hole; 37, first inner wall; 38, second inner wall; 39, third inner wall; 40, moving mechanism; 41, cam; 42, rotating shaft; 50, driving mechanism; 51, driving member; 52, transmission assembly; 53, first synchronous wheel; 54, second synchronous wheel; 55, synchronous belt; 56, rotating part; 57, operating part; 60, mounting platform. DETAILED DESCRIPTION

[0055] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0056] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0057] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0058] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0059] The disclosure below provides many different embodiments or examples for realizing different structures of the utility model. In order to simplify the disclosure of the utility model, the components and settings of specific examples are described below. Of course, they are merely examples, and the purpose is not to limit the utility model. In addition, the utility model may repeat reference numbers and / or reference letters in different examples, and such repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the utility model provides various specific examples of processes and materials, but those of ordinary skill in the art may be aware of the application of other processes and / or the use of other materials.

[0060] See also Figure 1-Figure 5 The carrier device 100 of the embodiment of the present application includes a carrier platform 10, a flow pool assembly 20, a fluid connection assembly 30 and a moving mechanism 40. The carrier platform 10 includes a table top 11. The flow pool assembly 20 is detachably located on the table top 11. The fluid connection assembly 30 is connected to the moving mechanism 40. When the moving mechanism 40 is working, it drives the fluid connection assembly 30 to move between a first position and a second position. When the fluid connection assembly 30 is located at the first position, the fluid connection assembly 30 forms a fluid connection with the flow pool assembly 20; when the fluid connection assembly 30 is located at the second position, the connection between the fluid connection assembly 30 and the flow pool assembly 20 is disconnected.

[0061] In the carrier device 100 of the embodiment of the present application, the fluid connection component 30 is driven to move relative to the flow pool component 20 by the motion mechanism 40, so that the connection and disconnection between the fluid connection component 30 and the flow pool component 20 can be achieved. The carrier device 100 has a simple structure and is easy to operate.

[0062] Specifically, the carrier platform 10 is used to carry the flow cell assembly 20. The carrier platform 10 may be in the shape of a cuboid, the table top 11 may be in the shape of a rectangle, and the size of the table top 11 may be smaller than the size of the flow cell assembly 20. The flow cell assembly 20 may be fixed on the table top 11 by a detachable connection method such as vacuum adsorption, magnetic adsorption, bonding, or clamping.

[0063] In some embodiments, the carrier device 100 may be provided with a temperature control component, which is at least partially in contact with the carrier platform 10. By providing the temperature control component to cooperate with the carrier platform 10, the temperature control component can detect and control the temperature of the carrier platform 10 to control the temperature of the flow pool component 20.

[0064] The fluid connection assembly 30 can be located at both ends of the carrier 10 and on one side of the flow cell assembly 20 close to the carrier 10. The fluid connection assembly 30 can move relative to the flow cell assembly 20 to connect and separate from the flow cell assembly 20. When the fluid connection assembly 30 is connected to the flow cell assembly 20, the fluid connection assembly 30 and the flow cell assembly 20 form a fluid connection; when the fluid connection assembly 30 is separated from the flow cell assembly 20, the connection between the fluid connection assembly 30 and the flow cell assembly 20 is disconnected. The first position is the position of the fluid connection assembly 30 when the fluid connection assembly 30 is connected to the flow cell assembly 20, and the second position is the position of the fluid connection assembly 30 when the fluid connection assembly 30 is separated from the flow cell assembly 20.

[0065] The motion mechanism 40 can be located on the side of the fluid connection component 30 away from the flow pool component 20. The motion mechanism 40 can be a cam mechanism, a crank slider mechanism, a gear rack mechanism, etc. The motion mechanism 40 can drive the fluid connection component 30 to perform reciprocating linear motion between the first position and the second position.

[0066] See also Figure 4 and Figure 6 In some embodiments, the flow pool assembly 20 includes a flow pool 21, and a fluid inlet 22 and a fluid outlet 23 are respectively arranged at both ends of the flow pool 21. The fluid connection assembly 30 includes a manifold 31 arranged corresponding to the fluid inlet 22 and the fluid outlet 23. The fluid connection assembly 30 and the flow pool assembly 20 form a fluid connection with the fluid inlet 22 and the fluid outlet 23 through the manifold 31.

[0067] In this way, by connecting the fluid inlet 22 and the fluid outlet 23 to the manifold 31, the fluid connection assembly 30 and the flow cell assembly 20 can be connected to facilitate fluid circulation.

[0068] Specifically, the flow cell 21 is used to provide a biochemical reaction site. The flow cell 21 can be a chip. The flow cell 21 has a space for accommodating liquid, which can accommodate the target analyte so that the target analyte and the reaction liquid undergo a biochemical reaction. The fluid inlet 22 and the fluid outlet 23 can be round holes or square holes. The number of the fluid inlet 22 and the fluid outlet 23 can be multiple, such as two, three, four, etc., and the multiple fluid inlets 22 and the multiple fluid outlets 23 are arranged in a one-to-one correspondence.

[0069] The manifold 31 can be made of metal, plastic and other materials, and the manifold 31 can be integrally injection molded by two rectangular parallelepipeds. The manifold 31 can include a plurality of fluid channels 311, and the plurality of fluid channels 311 are arranged at intervals along the length direction of the manifold 31. The cross-sectional shape of the fluid channels 311 can be circular or square, and the fluid channels 311 can be bent. The number of manifolds 31 can be multiple, wherein the plurality of fluid channels 311 of some manifolds 31 are arranged in a one-to-one correspondence with the plurality of fluid inlets 22, and the plurality of fluid channels 311 of another manifold 31 are arranged in a one-to-one correspondence with the plurality of fluid outlets 23, and the fluid can enter the flow pool 21 through the fluid channels 311 and the fluid inlets 22, and the fluid can also flow out of the flow pool 21 through the fluid channels 311 and the fluid outlets 23.

[0070] See also Figure 4 In certain embodiments, the fluid connection assembly 30 includes a support member 32 on which the manifold 31 is disposed.

[0071] In this way, the support member 32 can drive the manifold 31 to move between the first position and the second position, thereby achieving connection and disconnection between the manifold 31 and the flow pool 21 .

[0072] Specifically, the support member 32 may be in a T-shaped structure, and the support member 32 and the manifold 31 may be fixedly connected by fasteners such as bolts and screws. The support member 32 and the manifold 31 may be formed with mounting holes 33, and the mounting holes 33 may be circular in shape. The number of mounting holes 33 may be multiple, such as two, three, four, etc., and the multiple mounting holes 33 are arranged at intervals along the length direction of the support member 32 and extend along the height direction of the support member 32, and the screws and bolts are screwed into the mounting holes 33. The number of the support members 32 may be multiple, and the multiple support members 32 are arranged one by one with the multiple manifolds 31.

[0073] See also Figure 4 and Figure 7In some embodiments, the fluid connection assembly 30 includes an elastic member 34 , which is connected to the manifold 31 . The elastic member 34 is used to keep the manifold 31 moving toward the flow pool 21 .

[0074] In this way, the elastic member 34 can provide elastic force to buffer the impact force between the flow pool 21 and the manifold 31 . At the same time, the elastic member 34 can provide elastic force to drive the manifold 31 and the flow pool 21 to press tightly to ensure the sealing performance between the flow pool 21 and the manifold 31 .

[0075] Specifically, the elastic member 34 can be an elastic element such as a spring, and the manifold 31 can be formed with a groove 35. The shape of the groove 35 can be circular. The number of the grooves 35 can be multiple, such as two, three, four, etc. The multiple grooves 35 are arranged at intervals along the length direction of the manifold 31 and extend along the height direction of the manifold 31. One end of the elastic member 34 abuts against the groove 35.

[0076] See also Figure 5 and Figure 8 In some embodiments, the motion mechanism 40 includes a cam 41 and a rotating shaft 42. The cam 41 is connected to the rotating shaft 42. The rotating shaft 42 can drive the cam 41 to rotate. The cam 41 is also connected to the support member 32. When the cam 41 rotates, it drives the manifold 31 on the support member 32 to move to the first position or the second position.

[0077] In this way, the cam 41 can be driven to rotate by rotating the rotating shaft 42 , thereby driving the support member 32 and the manifold 31 to move, thereby achieving connection and disconnection between the manifold 31 and the flow pool 21 .

[0078] Specifically, the cam 41 may be a component with a curved profile, and the cam 41 may rotate clockwise or counterclockwise around the central axis of the rotating shaft 42 along with the rotating shaft 42. The rotating shaft 42 may be a circular shaft, and the number of the cams 41 may be multiple, such as two, three, four, etc., and the multiple cams 41 are arranged in a one-to-one correspondence with the multiple support members 32. The size of the cam 41 may be designed according to the relative distance between the first position and the second position.

[0079] See also Figure 5 and Fig. 9 In some embodiments, a limiting hole 36 is provided on the support member 32, and a cam 41 is provided in the limiting hole 36. The limiting hole 36 cooperates with the cam 41, so that when the cam 41 rotates, it can drive the manifold 31 on the support member 32 to move to the first position or the second position.

[0080] In this way, the cam 41 is set in the limiting hole 36, which can limit the movement range of the support member 32, and further limit the movement range of the manifold 31, so that the manifold 31 can move between the first position and the second position, thereby realizing the connection and disconnection between the manifold 31 and the flow pool 21.

[0081] Specifically, the shape of the limiting hole 36 can be square or circular, and the size of the limiting hole 36 can be designed according to the size of the cam 41, so that the cam 41 can rotate in the limiting hole 36 and drive the support member 32 to move. There can be multiple limiting holes 36, and multiple limiting holes 36 are arranged in a one-to-one correspondence with multiple cams 41.

[0082] See also Figure 5 and Fig. 9 In some embodiments, the limiting hole 36 is square and includes a first inner wall 37 and a second inner wall 38. When the cam 41 abuts against the first inner wall 37, the cam 41 drives the manifold 31 away from the flow pool 21; when the cam 41 abuts against the second inner wall 38, the cam 41 drives the manifold 31 close to the flow pool 21.

[0083] In this way, by abutting the cam 41 against the first inner wall 37 and the second inner wall 38, the movement range of the support member 32 can be limited, and then the movement range of the manifold 31 can be limited, so that the manifold 31 can move between the first position and the second position, thereby achieving connection and disconnection between the manifold 31 and the flow pool 21.

[0084] Specifically, the angle formed between the first inner wall 37 and the second inner wall 38 may be 90°, the first inner wall 37 may be parallel to the table 11, and the second inner wall 38 may be perpendicular to the table 11. The size of the first inner wall 37 and the second inner wall 38 may be designed according to the size of the cam 41.

[0085] See also Figure 5 and Fig. 9 In some embodiments, the limiting hole 36 includes a third inner wall 39, which is arranged opposite to the first inner wall 37 and closer to the manifold 31 relative to the first inner wall 37. When the cam 41 abuts against the third inner wall 39, the cam 41 drives the manifold 31 to approach the flow pool 21.

[0086] In this way, the movement range of the support member 32 can be limited by the contact between the cam 41 and the third inner wall 39, thereby limiting the movement range of the manifold 31, so that the manifold 31 can move between the first position and the second position, thereby achieving connection and disconnection between the manifold 31 and the flow cell 21. In addition, when the elastic member 34 cannot provide an elastic force to drive the manifold 31 and the flow cell 21 to press against each other, the contact between the cam 41 and the third inner wall 39 can drive the manifold 31 and the flow cell 21 to press against each other, thereby ensuring the sealing performance between the flow cell 21 and the manifold 31.

[0087] Specifically, the angle formed between the third inner wall 39 and the second inner wall 38 may be 90°, and the third inner wall 39 may be parallel to the first inner wall 37. The size of the third inner wall 39 may be consistent with the size of the first inner wall 37.

[0088] See also Figure 5 In some embodiments, the carrying device 100 includes a driving mechanism 50, and the driving mechanism 50 is used to drive the rotating shaft 42 to rotate.

[0089] In this way, the driving mechanism 50 can drive the rotating shaft 42 to rotate, thereby driving the cam 41 to rotate, thereby driving the movement of the support member 32 and the manifold 31 to achieve connection and disconnection between the manifold 31 and the flow pool 21.

[0090] Specifically, the driving method of the driving mechanism 50 includes but is not limited to manual driving, electric driving, pneumatic driving, hydraulic driving, etc. The driving mechanism 50 can be a cylinder assembly, or a motor assembly, such as a motor and a screw rod, or a motor and a belt. The driving mechanism 50 can be connected to the rotating shaft 42 by fasteners such as bolts and screws.

[0091] See also Figure 5 In some embodiments, the driving mechanism 50 includes a driving member 51 and a transmission assembly 52 . The transmission assembly 52 connects the driving member 51 and the rotating shaft 42 . The driving member 51 is used to drive the rotating shaft 42 to rotate through the transmission assembly 52 .

[0092] In this way, the driving member 51 drives the rotating shaft 42 to rotate through the transmission assembly 52, thereby driving the cam 41 to rotate, thereby driving the movement of the support member 32 and the manifold 31 to achieve connection and disconnection between the manifold 31 and the flow pool 21.

[0093] Specifically, the driving member 51 can be a motor or a cylinder, and the driving member 51 can drive the transmission assembly 52 to move, thereby driving the rotating shaft 42 to rotate. The transmission assembly 52 can be fixedly connected to the driving member 51 and the rotating shaft 42 by fasteners such as bolts and screws.

[0094] See also Figure 5 In some embodiments, the transmission assembly 52 includes a first synchronous wheel 53, a second synchronous wheel 54 and a synchronous belt 55, the first synchronous wheel 53 is connected to the driving member 51, the second synchronous wheel 54 is connected to the rotating shaft 42, and the synchronous belt 55 is wound around the first synchronous wheel 53 and the second synchronous wheel 54.

[0095] In this way, the rotating shaft 42 can be rotated through the transmission assembly 52, thereby driving the cam 41 to rotate relative to the flow pool 21, thereby driving the manifold 31 to be connected to and disconnected from the fluid of the flow pool 21.

[0096] Specifically, the first synchronous wheel 53 and the second synchronous wheel 54 can be made of materials such as steel, aluminum alloy, cast iron and brass, and the inner holes of the first synchronous wheel 53 and the second synchronous wheel 54 can be round holes, D-shaped holes and tapered holes. The inner circumferential surface of the synchronous belt 55 is provided with teeth with equal spacing with the first synchronous wheel 53 and the second synchronous wheel 54. When moving, the teeth of the synchronous belt 55 mesh with the tooth grooves of the first synchronous wheel 53 and the second synchronous wheel 54 to transmit motion and power. By setting the gear ratio of the first synchronous wheel 53 and the second synchronous wheel 54, the speed ratio of the second synchronous wheel 54 to the first synchronous wheel 53 can be adjusted.

[0097] After the driving member 51 is started, the driving member 51 drives the first synchronous wheel 53 to rotate, the tooth grooves of the first synchronous wheel 53 mesh with the teeth of the synchronous belt 55, the rotation of the first synchronous wheel 53 drives the synchronous belt 55 to move, the teeth of the synchronous belt 55 mesh with the tooth grooves of the second synchronous wheel 54, the movement of the synchronous belt 55 drives the second synchronous wheel 54 to rotate, the second synchronous wheel 54 is coaxially arranged with the rotating shaft 42, and the rotation of the second synchronous wheel 54 drives the rotating shaft 42 to rotate.

[0098] See also Figure 5 In some embodiments, the driving member 51 is a manual driving member, including a rotating portion 56 and an operating portion 57 connected to the rotating portion 56 , and the rotating portion 56 is connected to the first synchronous wheel 53 .

[0099] In this way, the rotation of the rotating part 56 and the first synchronous wheel 53 can be manually adjusted through the manual drive component, and the connection between the fluid connection component 30 and the flow pool component 20 can be manually controlled to achieve precise control of the fluid, with a simple structure, easy operation, and low manufacturing and maintenance costs.

[0100] Specifically, the rotating part 56 can be a cylinder, and the rotating part 56 extends outward along the diameter direction to form the operating part 57. The operating part 57 can be a cuboid. The rotating part 56 and the operating part 57 can be integrally formed or fixedly connected by welding. The rotating part 56 is coaxially arranged with the first synchronous wheel 53. The operating part 57 is manually operated to drive the rotating part 56 to rotate, thereby driving the first synchronous wheel 53 to rotate.

[0101] See also Figure 3 and Figure 5 In some embodiments, the number of the supporting platform 10 , the moving mechanism 40 and the driving mechanism 50 are all multiple and arranged in one-to-one correspondence, and the driving members 51 of the multiple driving mechanisms 50 are arranged on the same side of the supporting device 100 .

[0102] In this way, the number of the carrier 10, the motion mechanism 40 and the drive mechanism 50 are all multiple, and multiple flow cells 21 can be fixed at the same time, which is convenient for sequencing the samples to be tested on multiple flow cells 21 at the same time, thereby improving the sequencing efficiency. In addition, the drive members 51 of the multiple drive mechanisms 50 are arranged on the same side of the carrier device 100, which can reduce the space occupied by the drive members 51, making the structure of the carrier device 100 compact, thereby improving the space utilization of the carrier device 100 and reducing the volume of the carrier device 100.

[0103] Specifically, the number of the support platform 10, the motion mechanism 40 and the driving mechanism 50 can be two, the number of the flow pool assembly 20 and the fluid connection assembly 30 can also be two, the two support platforms 10 can fix the two flow pools 21 at the same time, so as to facilitate the processing and imaging of the two flow pools 21 at the same time, and the two driving mechanisms 50 respectively drive the two motion mechanisms 40 to move, thereby respectively driving the two fluid connection assemblies 30 to move relative to the two flow pools 21, so as to realize the separate control of the two fluid connection assemblies 30 and the two flow pools 21.

[0104] See also Figure 3 and Figure 5 In some embodiments, the carrying device 100 includes a mounting platform 60 , the carrying platform 10 and the moving mechanism 40 are respectively arranged on both sides of the mounting platform 60 , and the support member 32 is penetrated in the mounting platform 60 .

[0105] In this way, the mounting platform 60 is connected and fixed to the carrier platform 10, the motion mechanism 40 and the support member 32, which can improve the stability of the carrier device 100, and further improve the stability of the flow cell 21, thereby improving the sequencing accuracy.

[0106] Specifically, the shape of the mounting platform 60 can be a rectangular parallelepiped, the motion mechanism 40 can drive the support member 32 to move in the height direction relative to the mounting platform 60, the number of mounting platforms 60 can be two, and the two mounting platforms 60 are arranged in a one-to-one correspondence with the two supporting platforms 10 and the two motion mechanisms 40.

[0107] See also Figure 3 In some embodiments, at least one first groove 12 and at least one second groove 13 are provided on the table surface 11 of the supporting platform 10 , and the first groove 12 is connected to the second groove 13 .

[0108] In this way, by providing at least one first channel 12 and at least one second channel 13 in communication, a plurality of openings can be formed on the table 11 for adsorbing the flow cell 21 , thereby fixing the flow cell 21 on the table 11 .

[0109] Specifically, the number of the first groove 12 and the second groove 13 can be one, two, three, four, etc. When the number of the first groove 12 and the second groove 13 is more than one, the adsorption range of the table 11 can be further improved, thereby improving the stability of the carrier device 100 in adsorbing the flow cell 21. The cross-sectional shape of the first groove 12 and the second groove 13 can be rectangular or arc-shaped.

[0110] See also Figure 3 In some embodiments, the first groove 12 and the second groove 13 are arranged crosswise.

[0111] In this way, by arranging the first groove 12 and the second groove 13 in a cross manner, the coverage of the first groove 12 and the second groove 13 on the table 11 can be improved, which can be applicable to flow cells 21 of different sizes and improve the adsorption effect of the carrier 100.

[0112] Specifically, the angle between the first groove 12 and the second groove 13 may be an acute angle, a right angle, or an obtuse angle.

[0113] See also Figure 3 In some embodiments, there are multiple first grooves 12, and the multiple first grooves 12 are spaced apart and arranged in parallel, there are multiple second grooves 13, and the multiple second grooves 13 are spaced apart and arranged in parallel, and the first grooves 12 and the second grooves 13 are perpendicular to each other.

[0114] In this way, multiple first grooves 12 and multiple second grooves 13 are arranged vertically by crossing each other, so that the first grooves 12 and the second grooves 13 can form openings in a grid layout on the table 11. When the flow pool 21 is placed on the table 11, the flow pool 21 can be adsorbed and fixed by generating negative pressure at the openings of the first grooves 12 and the second grooves 13.

[0115] Specifically, the number of the first grooves 12 can be three, four, five, etc., and the number of the second grooves 13 can be five, six, seven, etc. The first grooves 12 can be parallel to the length direction of the table top 11, and the second grooves 13 can be parallel to the width direction of the table top 11; or the first grooves 12 can be parallel to the width direction of the table top 11, and the second grooves 13 can be parallel to the length direction of the table top 11.

[0116] In certain embodiments, the first channel 12 and / or the second channel 13 are connected to an external vacuum system.

[0117] In this way, the first groove 12 and / or the second groove 13 can generate negative pressure at the opening on the table 11, tightly joining the flow pool 21 to the table 11, with a good fixing effect, while improving the flatness of the flow pool 21 and reducing damage to the flow pool 21.

[0118] Specifically, the first channel 12 may be connected to an external vacuum system, the second channel 13 may be connected to an external vacuum system, or the first channel 12 and the second channel 13 may be connected to an external vacuum system. The external vacuum system includes but is not limited to a vacuum pump, a vacuum suction cup, and the like.

[0119] The sequencing system of the embodiment of the present application includes a carrier device 100 .

[0120] In this way, the sequencing system is convenient for detecting the flow cell 21 on the carrier device 100, and has convenient operation, high sequencing efficiency, and high detection accuracy. The sequencing system includes but is not limited to a nucleic acid sequence determination system.

[0121] In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiments or examples are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0122] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A carrying device, characterized in that: include: A carrying platform, the carrying platform comprising a table top; A flow cell assembly, the flow cell assembly being detachably located on the table; A fluid connection component and a motion mechanism, wherein the fluid connection component is connected to the motion mechanism, and when the motion mechanism is in operation, the fluid connection component is driven to move between a first position and a second position; When the fluid connection assembly is located at the first position, the fluid connection assembly is in fluid communication with the flow cell assembly; When the fluid connection assembly is located at the second position, the communication between the fluid connection assembly and the flow cell assembly is disconnected.

2. The carrying device according to claim 1, characterized in that: The flow cell assembly comprises a flow cell, and two ends of the flow cell are respectively provided with a fluid inlet and a fluid outlet; The fluid connection assembly includes a manifold arranged corresponding to the fluid inlet and the fluid outlet; The fluid connection assembly and the flow cell assembly are in fluid communication with the fluid inlet and the fluid outlet through the manifold.

3. The carrying device according to claim 2, characterized in that: The fluid connection assembly includes a support member on which the manifold is disposed.

4. The carrying device according to claim 3, characterized in that: The fluid connection assembly includes an elastic member connected to the manifold, and the elastic member is used to keep the manifold in a tendency to move toward the flow pool.

5. The carrying device according to claim 3 or 4, characterized in that: The motion mechanism comprises a cam and a rotating shaft; The cam is connected to the rotating shaft, and the rotating shaft can drive the cam to rotate; The cam is also connected to the support member, and when the cam rotates, it drives the manifold on the support member to move to the first position or the second position.

6. The carrying device according to claim 5, characterized in that: A limiting hole is arranged on the support member, and the cam is arranged in the limiting hole. The limiting hole cooperates with the cam, so that when the cam rotates, it can drive the manifold on the support member to move to the first position or the second position.

7. The carrying device according to claim 6, characterized in that: The limiting hole is square and includes a first inner wall and a second inner wall. When the cam abuts against the first inner wall, the cam drives the manifold away from the flow pool; When the cam abuts against the second inner wall, the cam drives the manifold to approach the flow pool.

8. The carrying device according to claim 7, characterized in that: The limiting hole comprises a third inner wall, the third inner wall is arranged opposite to the first inner wall and is arranged closer to the manifold than the first inner wall; When the cam abuts against the third inner wall, the cam drives the manifold to approach the flow pool.

9. The carrying device according to claim 5, characterized in that: The carrying device comprises a driving mechanism, and the driving mechanism is used for driving the rotating shaft to rotate.

10. The carrying device according to claim 9, characterized in that: The driving mechanism includes a driving member and a transmission assembly. The transmission assembly connects the driving member and the rotating shaft. The driving member is used to drive the rotating shaft to rotate through the transmission assembly.

11. The carrying device according to claim 10, characterized in that: The transmission assembly includes a first synchronous wheel, a second synchronous wheel and a synchronous belt, the first synchronous wheel is connected to the driving member, the second synchronous wheel is connected to the rotating shaft, and the synchronous belt is wound around the first synchronous wheel and the second synchronous wheel.

12. The carrying device according to claim 11, characterized in that: The driving member is a manual driving member, comprising a rotating part and an operating part connected to the rotating part, and the rotating part is connected to the first synchronous wheel.

13. The carrying device according to any one of claims 10 to 12, characterized in that: The number of the carrying platform, the movement mechanism and the driving mechanism are all multiple and are arranged in one-to-one correspondence, and the driving members of the multiple driving mechanisms are arranged on the same side of the carrying device.

14. The carrying device according to claim 3, characterized in that: The bearing device comprises a mounting platform, the bearing platform and the motion mechanism are respectively arranged on two sides of the mounting platform, and the support member is penetrated in the mounting platform.

15. The carrying device according to claim 1, characterized in that: At least one first groove and at least one second groove are provided on the surface of the supporting platform, and the first groove is connected to the second groove.

16. The carrying device according to claim 15, characterized in that: The first groove and the second groove are arranged crosswise.

17. The carrying device according to claim 15 or 16, characterized in that: There are multiple first grooves, and the multiple first grooves are arranged in parallel and at intervals. There are multiple second grooves, and the multiple second grooves are arranged in parallel and at intervals. The first grooves and the second grooves are perpendicular to each other.

18. The carrying device according to claim 15, characterized in that: The first channel and / or the second channel are connected to an external vacuum system.

19. A sequencing system, characterized in that: Comprising the carrying device according to any one of claims 1-18.

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