Detection target plate for microbial sample identification

Abstract

The utility model discloses a detection target plate for identifying a microorganism sample, which comprises a target plate main body, wherein the target plate main body is in a flat plate shape and is made of stainless steel; the stainless steel pipe is made of stainless steel and has the advantages of being resistant to weak corrosion media such as air, steam and water or stainless. The stainless steel has relatively high density and certain mass, so that the target plate main body can be effectively and stably placed on the identification platform and is not easy to loosen in the moving process, and the stability is enhanced; and the target plate main body is easy to process, so that the target plate surface is flat, and the precision is prevented from being influenced during detection. And the target plate main body made of stainless steel is hard in texture, not easy to damage and easy to clean.

Description

Technical Field

[0001] The utility model relates to a detection target plate for identifying microorganism samples, belonging to the field of detection target plates. Background Art

[0002] Matrix-assisted laser desorption / ionization time-offlight mass spectrometry (MALDI-TOF MS) technology, referred to as flight mass spectrometry technology, is one of the most representative technologies in the field of clinical microbial identification in recent years.

[0003] Each microorganism is composed of its own unique peptides / proteins. Through MALDI-TOF MS detection of microbial peptide / protein fingerprints, software processing and comparison analysis with the microbial database, the identification of microorganisms at the species and genus level can be completed within a few minutes. Compared with traditional methods for detecting microbial phenotypes and physiological and biochemical methods, MALDI-TOF MS has the advantages of being fast, accurate, high-throughput, and low-cost. In addition to identifying common bacteria and yeast-like fungi, it has greatly improved the efficiency and ability of clinical microbiology laboratories to identify difficult-to-identify microorganisms such as fastidious bacteria, anaerobic bacteria, filamentous fungi, and mycobacteria.

[0004] However, MALDI-TOF MS mass spectrometers cannot currently be used for direct detection of clinical specimens and mixed cultures. The objects of analysis must be pure microorganisms. Its high accuracy and repeatability in identifying microorganisms is based on the detection of microbial ribosomal proteins (relative molecular mass 2000-20000), which are a class of high-abundance proteins that are fixedly expressed in microbial cells. Therefore, in theory, different culture conditions will not lead to significant differences in the identification results of MALDI-TOF MS. However, in order to achieve more ideal identification results, laboratories should try to choose culture conditions that will not interfere with identification.

[0005] The pre-treatment step of MALDI-TOF MS identification is mainly to destroy the cell structure of microorganisms to achieve the purpose of inactivating microbial samples and extracting intracellular proteins. The structure and composition of microbial cell walls are the most important factors affecting the pre-treatment effect. Therefore, the sample wall breaking effect directly affects whether there is enough protein for mass spectrometry analysis, which is the prerequisite for determining whether the identification can be successful.

[0006] The detection target plate is a platform for placing the test sample. In the prior art, the target plate is mostly a split structure, which easily leads to an uneven target surface, thus affecting the sample results; and when cleaning the split target plate, cleaning liquid is easily left in the detection hole, which affects the accuracy of the detection. In addition, the split target plate is also prone to bending and damage, and has a short service life. Utility Model Content

[0007] In view of the deficiencies in the prior art, the utility model aims to provide a detection target plate for identifying microbial samples to solve the problem.

[0008] In order to achieve the above object, the utility model is implemented through the following technical scheme: a detection target plate for identifying microbial samples, comprising:

[0009] A target plate body, wherein the target plate body is in a flat plate shape and is made of stainless steel;

[0010] A target plate surface, which is arranged on the surface of the target plate body, and the target plate surface includes a frosted surface, a mirror surface, and a high-gloss surface;

[0011] The detection hole is arranged on the target plate surface. The detection hole is hemispherical and is used to store the detection sample. There are a plurality of detection holes, and the detection holes are distributed corresponding to the existing mass spectrometry rules.

[0012] Preferably, the detection holes are distributed in a rectangular matrix.

[0013] Preferably, a mark is provided on one side of the detection hole for locating the detection hole.

[0014] Preferably, the mark is arranged on one side of the detection holes in the first row and the first column.

[0015] Preferably, the target plate body is made of stainless steel with a chromium content of not less than 10.5% and a carbon content of not more than 1.2%.

[0016] Preferably, the detection hole is integrally formed with the target plate body.

[0017] Preferably, a recess is provided on both sides of the target plate body.

[0018] Preferably, the gap between adjacent detection holes is larger than the hole radius of the detection hole.

[0019] Beneficial Effects

[0020] The target plate body of the utility model is made of stainless steel, which has the characteristics of being resistant to weakly corrosive media such as air, steam, water, etc. or being rust-proof. In addition, stainless steel has a large density and a certain quality, which can effectively and stably place the target plate body on the identification platform, and is not easy to loosen during movement, thereby enhancing stability; and the target plate body is easy to be processed, so that the target plate surface is flat, avoiding affecting the accuracy during detection. In addition, the target plate body made of stainless steel is hard, not easy to be damaged, and easy to clean. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Other features, objects and advantages of the present invention will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0022] Figure 1 This is a schematic diagram of the structure of a detection target plate for identifying microbial samples according to the utility model;

[0023] Figure 2 For this utility model Figure 1 A schematic diagram of a top view structure;

[0024] Figure 3 It is a structural schematic diagram of the detection hole of the utility model. DETAILED DESCRIPTION

[0025] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0026] See also Figure 1-Figure 3 The utility model provides a technical solution of a detection target plate for identifying microbial samples: comprising a target plate body 1, a target plate surface 2, and a detection hole 3.

[0027] The target plate body 1 is plate-shaped and made of stainless steel. Preferably, the target plate body 1 is made of stainless steel with a chromium content of not less than 10.5% and a carbon content of not more than 1.2%, so as to improve the sensitivity and reproducibility of mass spectrometry. Carbon elements may produce specific signals in mass spectrometry, which may overlap with the mass spectrometry signals of the sample, resulting in an increase in background noise and affecting the detection of low-abundance signals. Therefore, it is more preferred that the carbon content of the target plate body 1 does not exceed 0.5%, such as 304L stainless steel. Among them, stainless steel is the abbreviation of stainless acid-resistant steel, which has the characteristics of being resistant to weak corrosive media such as air, steam, and water or having stainless properties. And stainless steel has a large density and a certain quality, which can effectively and stably place the target plate body 1 on the identification platform, and is not easy to loosen during movement, thereby enhancing stability.

[0028] The target plate surface 2 is arranged on the surface of the target plate body 1 and is integrally formed with the target plate body 1. The target plate surface 2 is processed into a frosted surface, a mirror surface or a high-gloss surface by an existing process. In this way, according to the characteristics of different samples, the target plate surface 2 of different processes can be selected to improve the dissociation and ionization efficiency of the sample; at the same time, the interference from the matrix ions in the background technology can be reduced.

[0029] The detection holes 3 are arranged on the target plate surface 2, and the detection holes 3 are provided in a plurality, and the detection holes 3 correspond to the existing mass spectrometry regular distribution. In one embodiment, the detection holes 3 are distributed in a rectangular matrix, so that the detection holes 3 correspond to the existing matrix-assisted laser desorption ionization time-of-flight mass spectrometer, so that the sample can be put on the machine for operation after spotting, and no additional components are required to assist in sample detection, which reduces unnecessary connection operations, optimizes the sample spotting process, reduces possible errors, and is easy to operate.

[0030] In order to simplify the operation steps, the target plate body 1 is made of the stainless steel mentioned above, so the target plate body 1 has good conductivity and can efficiently conduct electrical energy, thereby transferring it to the sample to be tested, thereby promoting the desorption and ionization of the sample to be tested; in one of the embodiments, when using this solution to deal with the MALDI-TOF mass spectrometer target plate, there is no need to add additional matrix during sample configuration, and high-sensitivity mass spectrometry detection can be achieved, thereby simplifying the operation steps, saving sample preparation time and greatly improving detection efficiency.

[0031] In one embodiment, 8 rows and 12 columns of detection holes 3 are provided, which are sufficient to store a plurality of detection samples.

[0032] In order to enable the inspector to quickly locate a certain detection hole 3 for sampling, a mark is set on one side of the detection hole 3. Preferably, the mark is set on the side of the detection hole 3 in the first row and the first column, so that the inspector can quickly locate a certain detection hole 3 according to the marks in the first row and the first column. In one embodiment, each detection hole 3 in the first row (top row) is marked with Arabic numerals at its upper left corner, and each detection hole 3 in the first column (leftmost column) is marked with letters at its lower right corner, so that a certain detection hole 3 can be quickly matched.

[0033] In order to facilitate the taking of the target plate body 1 , a recess 4 is provided on both sides of the target plate body 1 , so that after the inspection, the target plate body 1 can be carried by holding the recess 4 .

[0034] In another embodiment, if the amount of bacteria spotted is too small, it is easy to cause insufficient analysis signals, resulting in identification failure, and too much spotted bacteria or uneven coating may also lead to identification failure. Therefore, in order to avoid excessive spotted bacteria, resulting in cross-infection after sample overflow, the gap between adjacent detection holes 3 is larger than the hole radius of the detection hole 3, so that there is enough gap between adjacent detection holes 3, so that even if the sample overflows, it is controlled within a certain range and will not cause cross-infection. Figure 3 Preferably, the gap between adjacent detection holes 3 is defined as L, and the hole radius of the detection hole 3 is defined as R, wherein 3R>=L>R. More preferably, 2R>=L>=1.5R.

[0035] In order to avoid the uneven target surface affecting the sample detection results, the target plate body 1 is made of stainless steel and can be easily made into a flat plate by existing CNC machine tools, thereby avoiding errors caused by the uneven target surface.

[0036] In order to facilitate the cleaning of the target plate, the detection hole 3 is hemispherical, so that it is easier to clean after use and there is no dead corner.

[0037] Example 1

[0038] Take horse myocardial hemoglobin and bovine serum albumin standard solutions as an example:

[0039] 1. Mix the horse myocardial hemoglobin standard solution and CHCA matrix solution in an equal volume of 1:1, and take 2uL of the mixed solution for later use. 2. Dilute the bovine serum albumin standard solution to 1.3umol / L, mix it with sinapinic acid matrix in an equal volume of 1:1, and take 2uL of the mixed solution for later use;

[0040] Use an existing pipette to take 1uL of the microbial sample, drip it on the detection hole 3 in turn, spread it evenly, wait for it to dry, and then drip 1uL of CHCA matrix solution to cover it, and dry it; use a pipette to take 2uL of the horse myocardial hemoglobin and bovine serum albumin standard protein mixture and drip it on the detection hole 3, spread it evenly, wait for it to dry, and then it can be tested on the machine; in the machine detection, the CP Light-1000 microbial identification mass spectrometry system can be used for data processing. A 337nm laser with a frequency of 200Hz is used, the laser grating is set to 15, and the positive ion mode is used for detection. This detection method is not the only use scheme. In actual applications, it may need to be adjusted and optimized according to the specific situation to ensure the effectiveness and feasibility of the scheme. People in this field should conduct sufficient evaluation and testing before implementation to determine the most suitable scheme.

[0041] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0042] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A detection target plate for identification of microbial samples, characterized in that: include: A target plate body, wherein the target plate body is in a flat plate shape and is made of stainless steel; A target plate surface, which is arranged on the surface of the target plate body, and the target plate surface includes a frosted surface, a mirror surface, and a high-gloss surface; The detection hole is arranged on the target plate surface. The detection hole is hemispherical and is used to store the detection sample. There are a plurality of detection holes, and the detection holes are distributed corresponding to the existing mass spectrometry rules.

2. The detection target plate for identifying microbial samples according to claim 1, characterized in that: The detection holes are distributed in a rectangular matrix.

3. The detection target plate for identifying microbial samples according to claim 2, characterized in that: A mark is provided on one side of the detection hole for locating the detection hole.

4. The detection target plate for identifying microbial samples according to claim 3, characterized in that: The mark is arranged on one side of the detection hole in the first row and the first column.

5. The detection target plate for identification of microbial samples according to claim 1, characterized in that: The target plate body is made of stainless steel with a chromium content of not less than 10.5% and a carbon content of not more than 1.2%.

6. The detection target plate for identification of microbial samples according to claim 2, characterized in that: The detection hole is integrally formed with the target plate body.

7. The detection target plate for identification of microbial samples according to claim 1, characterized in that: A recess is provided on both sides of the target plate body.

8. The detection target plate for identification of microbial samples according to claim 1, characterized in that: The gap between adjacent detection holes is larger than the hole radius of the detection hole.

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