Centrifugal tube for extracting DNA (Deoxyribose Nucleic Acid) of plant tissue

Abstract

The utility model discloses a centrifuge tube for extracting DNA (deoxyribonucleic acid) of plant tissues, which belongs to the technical field of test articles and comprises a hollow tube body, two ends of the tube body are open, and a division indicating line is arranged on the tube body and divides the tube body into a grinding area and a separation area. A plurality of convex parts which are arranged in a staggered manner and are convenient for the grinding beads to grind plant materials are arranged in the grinding area; according to the utility model, the two ends of the hollow tube body are respectively connected with the caps, so that the interior of the tube body is sealed, and only the caps connected with the grinding area need to be opened when a grinding operation or a separation operation process is carried out; and the plant material grinding effect is improved through the protruding parts arranged in the grinding area, it is guaranteed that grinding and separation treatment do not conflict with each other through the two areas in the pipe body, and separation operation treatment is not affected while the grinding efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental supplies, in particular to a centrifuge tube used for extracting DNA from plant tissues. Background Art

[0002] The extraction of plant tissue DNA is mainly to analyze the gene structure, function and expression of the plant, understand the genetic characteristics and variation of the plant, and then identify the plant species and conduct ecological research. In the process of plant tissue DNA extraction, centrifuge tubes are often used, and centrifuge tubes play an important role in the whole process. They are used to hold plant tissue samples and various extraction reagents, and are indispensable in steps such as breaking cells and separating different components.

[0003] A fixed amount of plant material is selected and placed in a centrifuge tube, and then grinding beads are added to the centrifuge tube. A vibration device is then used to utilize the collision between the grinding beads and the plant material to crush the plant material. The grinding beads are then taken out, and additives are added. A centrifuge is used to separate the supernatant containing DNA from the lysate. However, after adding the grinding beads, when the plant material in the centrifuge tube is crushed using a vibration device, because the inner wall of the centrifuge tube is relatively smooth and the grinding beads are spherical, a grinding dead angle is generated between the grinding beads and the inner wall of the centrifuge tube when grinding the plant material, thereby reducing the efficiency of grinding the plant material and requiring a longer time to grind the plant material. Utility Model Content

[0004] The embodiment of the utility model provides a centrifuge tube for extracting DNA from plant tissues to solve the above-mentioned technical problems.

[0005] The utility model embodiment adopts the following technical solutions: including

[0006] A hollow tube body, both ends of which are open, a split indicator line is provided on the tube body, the split indicator line divides the tube body into a grinding area and a separation area, and the grinding area is provided with a plurality of staggered protrusions for facilitating grinding beads to grind plant materials;

[0007] A cover cap is movably connected to both ends of the tube body and is used to seal the tube body.

[0008] Furthermore, the raised portion is hemispherical with the curved surface facing the central axis of the tube body.

[0009] Furthermore, the raised portion is a vertical strip that curves toward the central axis of the tube body.

[0010] Furthermore, the raised portion is in the form of a transverse strip that curves toward the central axis of the tube body.

[0011] Furthermore, the diameter of the cap gradually decreases from a position close to the tube body to a direction away from the tube body.

[0012] Furthermore, the movable connection between the cap and the ports at both ends of the tube body is a threaded connection.

[0013] Furthermore, the movable connection between the cap and the tube body port near the grinding area is a snap connection.

[0014] At least one of the above technical solutions adopted in the embodiment of the utility model can achieve the following beneficial effects:

[0015] In the utility model, caps are respectively connected to the two ends of the hollow tube body to seal the inside of the tube body. When performing a grinding operation or a separation operation, it is only necessary to open the cap connected to the grinding area, and the raised portion arranged in the grinding area improves the grinding effect on the plant material. The two areas in the tube body ensure that there is no conflict during the grinding and separation processes, thereby improving the grinding efficiency without affecting the separation operation process, greatly saving the grinding time, and improving the grinding and separation process efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0017] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0018] Figure 2 This is a schematic structural diagram of the first embodiment of the raised portion of the utility model;

[0019] Figure 3 For the utility model Figure 2 The enlarged structural diagram at A in the middle;

[0020] Figure 4 This is a schematic structural diagram of a second embodiment of the raised portion of the utility model;

[0021] Figure 5 This is a schematic structural diagram of a third embodiment of the raised portion of the utility model;

[0022] Figure 6 This is a schematic diagram of the blocking structure of the utility model;

[0023] Figure 7 The utility model is a cross-sectional view Figure 2 ;

[0024] Figure 8For the utility model Figure 7 Enlarged structural diagram at B in the middle.

[0025] Reference numerals

[0026] Tube body 1, splitting indicator line 2, grinding area 201, separation area 202, cover cap 3, raised portion 4, stopper 7, elastic member 8. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be clearly and completely described below in combination with the specific embodiments of the utility model and the corresponding drawings. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] The technical solutions provided by various embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0029] The utility model provides a centrifuge tube for extracting DNA from plant tissues, comprising:

[0030] The hollow tube body 1 has openings at both ends. When the tube body 1 is in use, plant materials and grinding beads can be placed into the tube body 1 from both ends. A dividing indicator line 2 is provided on the tube body 1, and the dividing indicator line 2 divides the tube body 1 into a grinding area 201 and a separation area 202. The grinding area 201 is provided with a plurality of staggered protrusions 4 for facilitating the grinding of the plant materials by the grinding beads.

[0031] The cap 3 is movably connected to both ends of the tube body 1 and is used to seal the tube body 1. The cap 3 connected to the grinding area 201 is also provided with a raised portion 4 that is the same as that of the grinding area 201. The purpose is to facilitate the tube body 1 to achieve the same effect as the grinding area 201 during crushing and grinding. When the cap 3 connected to the separation area 202 is removed, the unground plant material and grinding beads can be placed into the tube body 1 through the exposed port of the tube body 1. Then the removed cap 3 is connected to the tank body, and then the tube body 1 is vibrated by a vibration device. During the vibration, the grinding beads and the plant material collide with each other, and the plant material is crushed in the process. Under the action of the raised portion 4, the friction between the grinding beads and the plant material is increased, thereby improving the contact state, strengthening the squeezing force between the grinding beads and the plant material, and also strengthening the effect of uniform grinding, thereby increasing the collision, squeezing and crushing of the plant material, so that in the same time, the grinding effect of the tube body 1 with the raised portion 4 is better than that of the tube body 1 without the raised portion 4.

[0032] After the grinding beads grind the plant material in the grinding area 201, the grinding beads are removed, and then the tube body 1 is re-sealed with the cap 3. At this time, the tube body 1 is turned upside down, and the crushed plant material will slide to the separation area 202. Then, the cap 3 connected to the separation area 202 is removed, and additives are added to the inside of the tube body 1 through the exposed port of the tube body 1. Then, the removed cap 3 is reconnected to the port of the tube body 1, and then the tube body 1 is placed in a centrifuge for centrifugal treatment.

[0033] The first embodiment of the raised portion 4 of the utility model is as follows Figure 2 As shown, the raised portion 4 is hemispherical with the curved surface facing the central axis of the tube body 1. When the grinding beads come into contact with the hemispherical raised portion 4, the hemispherical surface can ensure a relatively uniform contact area with the grinding balls, so that the collision force can be more evenly distributed in the contact area, which helps to achieve a smoother and more effective grinding action.

[0034] The second embodiment of the raised portion 4 of the utility model is as follows Figure 4 As shown, the raised portion 4 is a vertical bar that curves toward the central axis of the tube body 1. When the grinding beads collide with the raised portion 4 in the shape of a vertical bar, the contact area between the grinding beads and the inner wall of the tube body 1 will be increased, thereby increasing the grinding area of ​​the plant material. The vertical bar will guide the grinding beads to vibrate up and down with a larger amplitude. During this process, the plant material will be flipped over, and a turbulent effect will be formed locally, further enhancing the interaction between the grinding balls and the material, improving the grinding efficiency, and thereby increasing the grinding time of the plant material and improving the grinding effect of the plant material.

[0035] The third embodiment of the raised portion 4 of the utility model is as follows Figure 5 As shown, the raised portion 4 is a transverse bar that curves toward the central axis of the tube body 1. When the grinding beads collide with the transverse bar-shaped raised portion 4, the contact area between the grinding beads and the inner wall of the tube body 1 and the grinding area with the plant material will increase, and the transverse bar will guide the grinding beads to move laterally with a larger amplitude, so that the ground plant material and the unground plant material are fully mixed, thereby increasing the grinding time of the plant material and improving the grinding effect of the plant material.

[0036] Among them, the diameter of the cap 3 gradually decreases from the position close to the tube body 1 to the direction away from the tube body 1. The purpose is that when the tube body 1 is filled with plant materials, the plant materials will naturally and evenly precipitate and gather at the closed bottom of the tube body 1, so that when additives are added to the tube body 1, the plant materials and the additives can be better mixed, and when the plant tissue DNA mixture is separated, it is better to separate.

[0037] In a first embodiment of the connection method of the cap 3 of the utility model, the movable connection method between the cap 3 and the two end ports of the tube body 1 is a threaded connection. When the cap 3 and the tube body 1 are threadedly connected, the threaded connection method can ensure that when the tube body 1 is separated, the stability of the tail of the tube body 1 is ensured, so that the cap 3 will not be easily separated from the tube body 1. The threaded connection method ensures the stability of the connection between the cap 3 and the tube body 1.

[0038] The second embodiment of the connection method of the cap 3 of the utility model is as follows: Figure 7 as well as Figure 8 As shown, the movable connection between the cap 3 and the end of the tube body 1 near the grinding area 201 is a snap connection. In order to facilitate the installation and removal of the cap 3 connected to the grinding area 201, the cap 3 and the tube body 1 can be snap-connected, and an elastic member 8 is provided on the cap 3, and the elastic member 8 is in active contact with the outer wall of the tube body 1, thereby increasing the friction between the cap 3 and the tube body 1, thereby making the cap 3 more firmly connected to the tube body 1. When in use, it is only necessary to pull out the cap 3 connected to the grinding area 201 to open one end of the tube body 1. While being convenient to use, it also saves time to remove the cap 3, and does not affect the processing of the tube body 1 during the grinding operation and the centrifugal operation. In order to make the tube body 1 more stable when separated, a stopper 7 is connected to the position of the tube body 1 near the grinding area 201, which is used to replace the cap 3 with the centrifugal device for snap connection. When placed in the centrifuge, the snap-connected cap 3 will be detached from the tube body 1.

[0039] The above description is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the scope of the claims of the present invention.

Claims

1. A centrifuge tube for extracting DNA from plant tissues, characterized in that: include A hollow tube body (1), both ends of the tube body (1) are open, a dividing indicator line (2) is provided on the tube body (1), the dividing indicator line (2) divides the tube body (1) into a grinding area (201) and a separation area (202), and a plurality of staggered protrusions (4) are provided in the grinding area (201) for facilitating grinding beads to grind plant materials; A cover cap (3), the cover cap (3) being movably connected to both ends of the tube body (1) and used to seal the tube body (1).

2. A centrifuge tube for extracting DNA from plant tissue according to claim 1, characterized in that: The raised portion (4) is in the shape of a hemisphere with a curved surface facing the central axis of the tube body (1).

3. A centrifuge tube for extracting DNA from plant tissue according to claim 1, characterized in that: The raised portion (4) is in the shape of a vertical strip that curves toward the central axis of the tube body (1).

4. A centrifuge tube for extracting DNA from plant tissue according to claim 1, characterized in that: The raised portion (4) is in the form of a transverse strip that curves toward the central axis of the tube body (1).

5. A centrifuge tube for extracting DNA from plant tissue according to claim 1, characterized in that: The diameter of the cover cap (3) gradually decreases from a position close to the tube body (1) towards a direction away from the tube body (1).

6. A centrifuge tube for extracting DNA from plant tissue according to claim 1, characterized in that: The movable connection between the cover cap (3) and the two end ports of the tube body (1) is a threaded connection.

7. A centrifuge tube for extracting DNA from plant tissue according to claim 1, characterized in that: The movable connection between the cover cap (3) and the end of the tube body (1) close to the grinding area (201) is achieved by snap-fitting.

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