Polysaccharide polar separation system

Abstract

The utility model provides a polysaccharide polarity separation system which comprises a sample introduction unit, a solvent unit, an elution unit, a multi-position pump, a separation column, a sample pool and a detector, the sample introduction unit, the solvent unit and the elution unit are respectively connected with the liquid inlet end of the separation column through the multi-position pump, and the liquid outlet end of the separation column is connected with the sample pool. And a sample in the sample pool is conveyed to the detector through the infusion pump. Multi-position pump switching is adopted, automation of the whole process of online liquid preparation, online elution, online detection and online collection is achieved through an upper computer system customized by the company, unmanned operation of the instrument can be achieved, 24-hour online operation can be achieved, the working efficiency of researchers is improved, automatic control is achieved, and the working efficiency of the researchers is improved. The stability of the whole process is enhanced, so that the stability of a polysaccharide component detection result is improved, and the efficiency is greatly improved.

Description

Technical Field

[0001] The utility model relates to a separation system, in particular to a polysaccharide polarity separation system. Background Art

[0002] Polysaccharides are a kind of biological macromolecules. According to the different charges of polysaccharides, they can be divided into neutral polysaccharides, acidic polysaccharides and alkaline polysaccharides. Conventional separation uses ion exchange fillers for separation, and manually prepares sodium chloride elution solutions with different gradient concentrations for elution. The received components are collected tube by tube using the phenol-sulfuric acid method or the anthrone-sulfuric acid method and tested. In this process, it is almost entirely operated manually, which wastes the precious time of researchers and also has a certain impact on the stability of the results. Based on the above problems, our company has developed a polysaccharide polarity separation system. Utility Model Content

[0003] The purpose of the utility model is to provide a polysaccharide polarity separation system to solve the problems raised in the above background technology.

[0004] In order to solve the above technical problems, the utility model provides the following technical solutions: a polysaccharide polarity separation system, comprising an injection unit, a solvent unit, an elution unit, a multi-position pump, a separation column, a sample pool and a detector, wherein the injection unit, the solvent unit and the elution unit are respectively connected to the liquid inlet end of the separation column through the multi-position pump, and the liquid outlet end of the separation column is connected to the sample pool, and the sample in the sample pool is transported to the detector through an infusion pump.

[0005] Furthermore, it also includes a splitter and a collector, and the liquid outlet end of the separation column is connected to the sample pool and the collector respectively through the splitter.

[0006] Furthermore, it also includes a display unit, and the display unit is connected to the sample pool and then connected to the detector.

[0007] Furthermore, the solvent unit comprises a pure water injector and an ionic liquid injector, wherein the pure water injector and the ionic liquid injector are respectively provided with pure water and ionic solution.

[0008] Furthermore, a sodium hydroxide solution is provided in the elution unit.

[0009] Furthermore, the display unit includes a first display pool and a second display pool, and reagent A and reagent B are respectively disposed in the first display pool and the second display pool.

[0010] Furthermore, the detector is a full wavelength scanning detector or an ultraviolet detector.

[0011] Compared with the prior art, the beneficial effects achieved by the utility model are:

[0012] 1. This application adopts multi-position pump switching, and realizes the automation of the whole process of online liquid preparation, online elution, online detection and online collection through the customized upper computer system of our company. The instrument can realize unmanned operation and can realize 24-hour online operation, which improves the work efficiency of scientific researchers. The automated control enhances the stability of the whole process, thereby improving the stability of the polysaccharide component detection results and greatly improving the efficiency;

[0013] 2. The polysaccharide polarity separation system of the present application adopts a modular design. The system is easy to maintain and provides researchers with flexibility to add or delete functions according to their needs in the future, thereby fully and efficiently utilizing instruments and equipment and saving valuable time costs. The pump module is made of peek material, which is resistant to alkali, salt and corrosion, and fully considers the application environment of researchers to extend the service life. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. In the accompanying drawings:

[0015] Figure 1 This is a schematic structural diagram of Example 1 of a polysaccharide polarity separation system of the utility model;

[0016] Figure 2 This is a schematic structural diagram of Example 2 of a polysaccharide polarity separation system of the utility model;

[0017] In the figure: 1 injection unit; 2 solvent unit; 21 pure water injector; 22 ionic liquid injector; 3 elution unit; 4 multi-position pump; 5 separation column; 6 splitter; 7 sample cell; 8 collector; 9 display unit; 91 first display cell; 92 second display cell; 10 detector. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] Example 1

[0020] See also Figure 1A polysaccharide polarity separation system comprises an injection unit 1, a solvent unit 2, an elution unit 3, a multi-position pump 4, a separation column 5, a splitter 6, a sample pool 7, a collector 8, a display unit 9 and a detector 10. The injection unit 1, the solvent unit 2 and the elution unit 3 are respectively connected to the liquid inlet end of the separation column 5 by the multi-position pump 4. The set amount of polysaccharide sample and auxiliary liquid are respectively output by the injection unit 1 and the solvent unit 2 through the infusion pump, and are mixed by the multi-position pump 4 and transported to the separation column 5 through a pipeline to separate different polysaccharide components. The different polysaccharide components are output from the separation column 5 in chronological order. Each component is split into a set trace amount and a residual amount by the splitter 6. The residual amount enters the collector 8, and the trace amount of the component enters the sample pool 7. The polysaccharide in the sample pool 7 The components and the indicator in the display unit 9 are quantitatively mixed by the output of the infusion pump, and enter the detector 10 through the pipeline through the diverter 6 for component detection. The detector 10 is a full-wavelength scanning detector or an ultraviolet detector. After the detection of the various different components separated from the polysaccharide is completed, the eluent in the elution unit 3 is transported by the infusion pump and input into the separation column 5 by the multi-position pump 4 to clean its internal impurities, and the cleaning liquid enters the collector 8 after being discharged from the separation column 5. The above separation process adopts the switching of the multi-position pump 4, and the whole process automation of online liquid preparation, online elution, online detection and online collection is realized through the customized upper computer system of our company. This instrument system can realize unmanned operation and can realize 24-hour online operation to fully and efficiently utilize the equipment.

[0021] Example 2

[0022] See also Figure 2 On the basis of the technical solution of Example 1, the solvent unit 2 includes a pure water injector 21 and an ion liquid injector 22, wherein the pure water injector 21 and the ion liquid injector 22 are respectively provided with pure water and ion solution, and the gradient ratio of pure water and ion solution can be realized through the upper computer system customized by our company, and the ion water solution after gradient ratio and the polysaccharide sample are respectively quantitatively output and mixed by the infusion pump and then transported to the separation column 5 by the pipeline to separate different polysaccharide components, and the sodium hydroxide solution provided in the elution unit 3 washes away the salt substances in the separation column 5, thereby ensuring the column efficiency of the separation column 5 and ensuring the accuracy of the entire detection result. Stability, the display unit 9 includes a first display pool 91 and a second display pool 92, and reagents A and B are respectively arranged in the first display pool 91 and the second display pool 92, and reagents A and reagent B are conventional detection reagents in the field, which are common knowledge of those skilled in the art. The upper computer system of our company can also realize the gradient ratio of reagents A and reagents B. The display agent after gradient ratio and the polysaccharide components in the sample pool 7 are quantitatively transported to the detector 10 for sample component detection through an infusion pump, and the display agent formed by the mixture of reagents A and reagent B can enhance the detection signal of the polysaccharide component, thereby ensuring the effect of polysaccharide component detection.

[0023] It should be noted that relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises", or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article, or device.

[0024] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A polysaccharide polarity separation system, characterized in that: The invention comprises an injection unit, a solvent unit, an elution unit, a multi-position pump, a separation column, a sample pool and a detector. The injection unit, the solvent unit and the elution unit are respectively connected to the liquid inlet end of the separation column through the multi-position pump, and the liquid outlet end of the separation column is connected to the sample pool. The sample in the sample pool is transported to the detector through an infusion pump.

2. A polysaccharide polarity separation system according to claim 1, characterized in that: It also includes a splitter and a collector, and the liquid outlet end of the separation column is connected to the sample pool and the collector respectively through the splitter.

3. A polysaccharide polarity separation system according to claim 1 or 2, characterized in that: It also includes a display unit, which is connected to the detector after being connected to the sample pool.

4. A polysaccharide polarity separation system according to claim 1, characterized in that: The solvent unit comprises a pure water injector and an ion liquid injector, wherein the pure water injector and the ion liquid injector are respectively provided with pure water and ion solution.

5. A polysaccharide polarity separation system according to claim 1, characterized in that: The elution unit is provided with a sodium hydroxide solution.

6. A polysaccharide polarity separation system according to claim 3, characterized in that: The display unit includes a first display pool and a second display pool, wherein reagent A and reagent B are respectively disposed in the first display pool and the second display pool.

7. A polysaccharide polarity separation system according to claim 1, characterized in that: The detector is a full wavelength scanning detector or an ultraviolet detector.

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