Portable immune micro-fluidic detector

Abstract

The utility model relates to a portable immune micro-fluidic detector, which comprises a detector body and is characterized in that the detector body comprises a shell, and a micro-fluidic chip reaction area, a reagent bag placing area, a liquid supply mechanism and a bag scratching mechanism capable of scratching a reagent bag are arranged in the shell. The detection instrument has the advantages that the micro-fluidic chip reaction area, the reagent bag placing area and the bag scratching mechanism are respectively and integrally arranged in the detection instrument, and the micro-fluidic chip reaction area and the reagent bag placing area can be respectively and detachably inserted through own sockets; rapid home immunodetection can be conveniently performed based on the micro-fluidic chip, the reagent grooves for placing the reagent bags are communicated through the connecting channels, and the bags are scratched one by one under the action of the bag scratching mechanism to perform multi-stage reaction, so that the sample adding speed can be increased, and the tedious problem caused by repeated sample adding can be avoided; and the size and the manufacturing cost of the detector can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic instant detection, in particular to a microfluidic chip detector capable of improving integration. Background Art

[0002] With the development of science and technology, the demand for home health testing has increased, and the demand for home testing has expanded, making POCT testing one of the main development directions in the field of testing in recent years. POCT refers to on-site sampling, on-site detection of results, and the use of portable and compact analytical instruments and supporting reagents to quickly obtain the target test object. For example, the Chinese invention patent application "POCT microfluidic chip, detection system, detection method and application" of the existing patent application CN202210493174.0 (publication number CN115007228A) provides a quick, lightweight and compact chip, which solves the problem that the reaction liquid needs to obtain the required temperature in each constant temperature chamber in the reaction pool, but there is only one inlet connected to the reaction pool, so that the sample must be added to each corresponding inlet according to the order of the reaction, which is relatively cumbersome in operation. In addition, at present, home detectors have not yet appeared significantly in the public eye. Therefore, the market needs a low-cost, fast and simple, safe and reliable, disposable detection device that can achieve precise control of the sample addition and detection process, and can be used without professional training. Utility Model Content

[0003] The technical problem to be solved by the utility model is to provide a detector which is easy to operate and can perform rapid home immune detection based on a microfluidic chip in response to the above-mentioned existing technical status.

[0004] The technical solution adopted by the utility model to solve the above technical problems is: the portable immunomicrofluidic detector includes an instrument body, characterized in that: the instrument body includes a shell, and the shell is provided with:

[0005] The reaction area of ​​the microfluidic chip includes a substrate and a mixing chamber arranged on the substrate, wherein at least two mixing chambers are arranged at intervals, each mixing chamber is connected to a reaction chamber downstream, each mixing chamber is provided with an inlet for liquid inlet upstream, and an outlet for liquid outlet is provided on the reaction chamber located at the most downstream;

[0006] Features:

[0007] A first socket is provided on the housing and can be inserted into the substrate in a detachable manner;

[0008] A reagent pack placement area is located on the substrate, comprising a base and reagent slots arranged in the base for placing reagent packs to be reacted, each reagent slot is connected through a connecting channel, the upstream of each connecting channel is connected to an upstream channel, and the ends of each connecting channel merge into a common confluence section, and the confluence section is fluidically connected to the inlet on the substrate;

[0009] A second socket is provided on the housing for the base to be removably inserted; and

[0010] The bag-scratching mechanism is located on the base platform and includes a knife capable of slashing the reagent bag containing the reaction solution and a driving component capable of driving the knife to move along the upstream channel and slash the reagent bags one by one.

[0011] In order to facilitate the integration of the stripping mechanism in a modular form within the shell, preferably, the stripping mechanism also includes a support assembly that supports the drive assembly, the support assembly includes a first support frame and a second support frame, and the drive assembly includes a drive source arranged on the first support frame and a transmission rod connected to the power output end of the drive source, the two ends of the transmission rod are respectively connected to the first support frame and the second support frame, and its setting direction is parallel to the upstream channel, and the stripping knife is connected to the transmission rod. Since the setting direction of the transmission rod is parallel to the upstream channel, it can drive the stripping knife to translate along the upstream channel, and then cut the reagent packs one by one, perform multi-stage reactions, and finally achieve efficient and rapid fluid mixing and reaction.

[0012] In order to prevent the cutter from rotating along the transmission rod under the action of the driving source, preferably, the first support frame and the second support frame are also provided with a limiting member that can limit the cutter to move only along the transmission rod. The function of the limiting member is to make the cutter translate only along the upstream channel.

[0013] From the perspective of structure and simple coordination with the first supporting frame and the second supporting frame, preferably, the limiting member is two limiting rods arranged parallel to the transmission rod, and correspondingly, the cutting knife includes a cutting knife seat connected to the transmission rod, and the cutting knife seat is provided with through holes for the two limiting rods to pass through respectively.

[0014] Furthermore, an injection pump drive area is also provided in the shell, and the injection pump drive area includes a cavity of the injection pump, a water inlet pipe arranged on the cavity and connected to the outlet of the microfluidic chip reaction area, and a water outlet pipe arranged on the cavity. The liquid is discharged from the water outlet pipe through the reciprocating motion of the piston in the injection pump.

[0015] In order to facilitate the cleaning of the waste liquid collection chamber, preferably, a waste liquid collection area connected to the water outlet pipe is also provided in the shell, and the waste liquid collection area includes a waste liquid collection chamber. The shell is provided with a first opening corresponding to the waste liquid collection area, and the first opening can allow the waste liquid collection chamber of the waste liquid collection area to be removably inserted. Correspondingly, a handle exposed on the first opening is provided on the waste liquid collection chamber.

[0016] In order to improve the reaction speed and reaction accuracy in the reaction area of ​​the microfluidic chip, preferably, a micro-vibrator capable of enhancing mixing and a photoelectric detector and a photoelectric receiver capable of detecting reaction concentration are also arranged in the shell on the reaction area of ​​the microfluidic chip.

[0017] In order to facilitate user operation, preferably, the shell is also provided with buttons that can control the above-mentioned microfluidic chip reaction area, injection pump driving area, bagging mechanism and waste liquid collection area.

[0018] Compared with the prior art, the utility model has the advantages that: a microfluidic chip reaction area, a reagent pack placement area and a bag-scratching mechanism are respectively integrated in the detector; in addition, the microfluidic chip reaction area and the reagent pack placement area can be detachably inserted through their own sockets, respectively, so that rapid home immunoassays based on microfluidic chips can be conveniently performed; finally, the reagent slots in which the reagent packs are placed are connected through connecting channels, and are cut one by one for multi-stage reactions under the action of the bag-scratching mechanism, which can increase the speed of sample addition and avoid the cumbersome problems caused by multiple sample additions; it can also reduce the size and cost of the detector, which is of great significance for achieving true instant detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the detector in the embodiment of the utility model;

[0020] Figure 2 It is a partial cross-sectional view of the detector in the embodiment of the utility model;

[0021] Figure 3 It is a cross-sectional view of the detector in the embodiment of the utility model (blade omitted);

[0022] Figure 4 This is a schematic diagram of the structure of the microfluidic chip in the embodiment of the utility model;

[0023] Figure 5 It is a cross-sectional view of the internal structure of the detector in the embodiment of the utility model;

[0024] Figure 6 for Figure 5 The structural schematic diagram of the shell is omitted;

[0025] Figure 7A cross-sectional view of a local angle of the detector in the embodiment of the utility model;

[0026] Figure 8 It is a cross-sectional view of a part of the detector in the embodiment of the utility model at another angle. DETAILED DESCRIPTION

[0027] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0028] like Figures 1 to 8 The portable immunomicrofluidic detector of this embodiment includes an instrument body 1, which includes a shell 11, and a microfluidic chip reaction area 2 is arranged in the shell 11, including a substrate 21 and a mixing chamber 22 arranged on the substrate 21, and at least two mixing chambers 22 are arranged at intervals, and each mixing chamber 22 is connected to a reaction chamber 23 downstream, and each mixing chamber 22 is provided with an inlet 24 for liquid inlet upstream, and an outlet 25 for liquid outlet is provided on the reaction chamber 23 located at the most downstream, and correspondingly, a first socket 12 is provided on the shell 11 for the substrate 21 to be removably inserted, so that the user can realize the replacement according to the use requirements. The focus of this embodiment is that the reaction liquid for liquid supply is integrated in the reagent package placement area 3, and the reagents containing the reaction liquid are scratched one by one by the bag scratching mechanism 4. The reagent package, specifically: the reagent package placement area 3 is integrated in the shell 11 at a position above the substrate 21, including a base 31 and a reagent tank 32 arranged in the base 31 for placing the reagent package to be reacted, each reagent tank 32 is connected through a connecting channel 33, and is connected to an upstream channel 34 upstream of each connecting channel 33, and the ends of each connecting channel 33 converge with a common confluence section 35, and the confluence section 35 is fluidically connected to the inlet 24 on the substrate 21. Of course, a second socket 13 into which the base 31 can be removably inserted is also provided on the shell 11. Correspondingly, the package cutting mechanism 4 is arranged at a position above the base 31, which includes a cutting knife 41 that can cut the reagent package containing the reaction solution, and a driving component that can drive the cutting knife 41 to move along the upstream channel 34 and cut the reagent packages one by one. In addition, the shell 11 also integrates the microfluidic chip reaction area 2, the bagging mechanism 4, the injection pump drive area 6, the waste liquid collection area 7 and the buttons 30 for controlling each area, which can conveniently perform rapid home immune detection based on the microfluidic chip. It is one of the important technical means to realize home health management in the future. It can also be associated with a smartphone APP account to realize the storage of health data, real-time and dynamic viewing of health data, establishment of personal health records, push of post-management intervention plans, and recommended diet and exercise plans.

[0029] In order to facilitate the integration of the bagging mechanism 4 in a modular form in the housing 11, the bagging mechanism 4 of this embodiment also includes a support assembly 5 for supporting the driving assembly, the support assembly 5 includes a first support frame 51 and a second support frame 52, and the driving assembly includes a driving source 42 arranged on the first support frame 51 and a transmission rod 43 connected to the power output end of the driving source 42, the two ends of the transmission rod 43 are respectively connected to the first support frame 51 and the second support frame 52, and the setting direction is parallel to the upstream channel 34, and the blade 41 is connected to the transmission rod 43, since the transmission rod 43 is set in a direction parallel to the upstream channel 34, it can drive the cutting knife 41 to translate along the upstream channel 34, and then cut through the reagent packages one by one, perform multi-stage reactions, and finally achieve efficient and rapid fluid mixing and reaction. In addition, the first support frame 51 and the second support frame 52 are also provided with a limiting member that can limit the movement of the cutting knife 41 only along the transmission rod 43. The function of the limiting member is to make the cutting knife 41 translate only along the upstream channel 34, thereby preventing the cutting knife 41 from rotating along the transmission rod 43 under the action of the driving source 42. In this embodiment, the limiting member is considered to be two limiting rods 44 arranged parallel to the transmission rod 43 from the perspective of structure and simple coordination with the first support frame 51 and the second support frame 52. Correspondingly, the cutting knife 41 includes a cutting knife seat 40 connected to the transmission rod 43, and the cutting knife seat 40 is provided with through holes 401 for the two limiting rods 44 to pass through respectively, and an injection pump driving area 6 is also arranged in the shell 11. The injection pump driving area 6 includes a cavity 61 of the injection pump, a water inlet pipe 62 arranged on the cavity 61 and connected to the outlet 25 of the microfluidic chip reaction area 2, and a water outlet pipe 63 arranged on the cavity 61. The liquid is discharged from the water outlet pipe 63 by the reciprocating motion of the piston in the injection pump. A waste liquid collection area 7 connected to the water outlet pipe 63 is also provided in the shell 11. The waste liquid collection area 7 includes a waste liquid collection chamber 71. The shell 11 is provided with a first opening 14 corresponding to the waste liquid collection area 7. The first opening 14 can be used for the waste liquid collection chamber 71 of the waste liquid collection area 7 to be removably inserted. Correspondingly, a handle 72 exposed on the first opening 14 is provided on the waste liquid collection chamber 71. The user can easily clean the waste liquid collection chamber 71 by pulling the handle 72. Finally, in order to improve the reaction speed and reaction accuracy in the microfluidic chip reaction area 2, a micro-vibrator 8 that can enhance mixing and a photoelectric detector 9 and a photoelectric receiver 10 that can detect the reaction concentration are also provided in the shell 11 on the microfluidic chip reaction area 2.

[0030] At the same time, a magnet 20 is provided at the bottom of the reaction chamber 23, and the magnet 20 can adsorb the conjugate produced by the immune reaction into the reaction chamber 23. Generally, in immune reactions, reagent balls having capture reagent balls and labeling reagent balls are often used, wherein the capture reagent balls have magnetic beads coated with antibodies, antigens or secondary antibodies, and the labeling reagent balls are fluorescent microspheres coated with antibodies, antigens or secondary antibodies; the sample enters the mixing chamber 22 to dissolve the reagent balls to obtain a mixed sample, and the mixed sample enters the reaction chamber 23 to generate an immune reaction. At this time, the magnet 20 located at the bottom of the reaction chamber 23 adsorbs the conjugate of magnetic beads coated with antibodies, antigens or secondary antibodies produced by the immune reaction into the reaction chamber 23, and the reaction chamber 23 only contains the adsorbed magnetic bead conjugate.

[0031] In summary, by integrating the microfluidic chip reaction area 2, the reagent pack placement area 3 and the liquid supply mechanism in the detector, in addition, the microfluidic chip reaction area 2 and the reagent pack placement area 3 can be detachably inserted through their own sockets, so that rapid home immune detection based on the microfluidic chip can be conveniently performed. The detection process of the reagent pack in combination with the microfluidic chip is as follows (taking reagent packs No. 1, No. 2, No. 3 and No. 4 as examples):

[0032] ①, the substrate 21 corresponding to the reaction area 2 of the microfluidic chip is inserted from the first socket 12, and at the same time, the base 31 containing the No. 1, No. 2, No. 3 and No. 4 reagent packs is inserted from the second socket 13. When the user operates the corresponding button 30, the bag-scratching mechanism 4 can be driven to make the knife 41 slash the No. 1 reagent pack containing the reaction solution along the upstream channel 34;

[0033] ② The reaction liquid can flow along the ends of the connecting channels 33 to the common confluence section 35 and enter the inlet 24 on the substrate 21 to contact with the sample;

[0034] ③. When you hear the beep, press the detection switch

[0035] ④. Trigger the micro vibrator 8 and vibrate and incubate for 5 minutes;

[0036] ⑤. Remove the micro vibrator 8, switch the electromagnet 20, and touch the bottom for 3 seconds;

[0037] ⑥. Cut open the No. 2 reagent pack and drain all the liquid inside the chip to the waste liquid collection area 7;

[0038] ⑦. Remove the electromagnet 20 and push in the micro vibrator 8;

[0039] ⑧. Scratch the No. 3 reagent pack and incubate for 5 minutes;

[0040] ⑨. Remove the micro vibrator 8, switch the electromagnet 20, and touch the bottom for 3 seconds;

[0041] ⑩. After cutting open the No. 4 reagent package, push all the liquid into the reaction chamber 23. The reaction chamber 23 detects the values ​​of different absorbances and displays the data on the LCD. At the same time, your test data results will pop up on the mobile phone APP.

Claims

1. A portable immunomicrofluidic detector, comprising an instrument body (1), characterized in that: The instrument body (1) comprises a housing (11), and the housing (11) is provided with: A microfluidic chip reaction zone (2), comprising a substrate (21) and a mixing chamber (22) arranged on the substrate (21), wherein at least two mixing chambers (22) are arranged at intervals, each mixing chamber (22) is connected to a reaction chamber (23) downstream, each mixing chamber (22) is provided with an inlet (24) for liquid inlet upstream, and an outlet (25) for liquid outlet is provided on the reaction chamber (23) located at the most downstream; Features: A first socket (12) is provided on the housing (11) and is capable of allowing the substrate (21) to be removably inserted therein; A reagent pack placement area (3), located on the substrate (21), comprises a base (31) and reagent slots (32) arranged in the base (31) for placing reagent packs to be reacted, each reagent slot (32) is connected via a connecting channel (33), the upstream of each connecting channel (33) is connected to an upstream channel (34), and the ends of each connecting channel (33) merge into a common confluence section (35), and the confluence section (35) is fluidically connected to an inlet (24) on the substrate (21); A second socket (13) is provided on the housing (11) for the base (31) to be removably inserted; and The bag-scratching mechanism (4) is located on the base (31) and comprises a knife (41) capable of slashing a reagent bag containing a reaction solution, and a driving component capable of driving the knife (41) to move along the upstream channel (34) and slash the reagent bags one by one.

2. The portable immunomicrofluidic detector according to claim 1, characterized in that: The striping mechanism (4) also includes a supporting assembly (5) for supporting the driving assembly, the supporting assembly (5) includes a first supporting frame (51) and a second supporting frame (52), and the driving assembly includes a driving source (42) arranged on the first supporting frame (51) and a transmission rod (43) connected to the power output end of the driving source (42), the two ends of the transmission rod (43) are respectively connected to the first supporting frame (51) and the second supporting frame (52), and the setting direction thereof is parallel to the upstream channel (34), and the striping knife (41) is connected to the transmission rod (43).

3. The portable immunomicrofluidic detector according to claim 2, characterized in that: The first support frame (51) and the second support frame (52) are also provided with a limiting member capable of limiting the blade (41) to move only along the transmission rod (43).

4. The portable immunomicrofluidic detector according to claim 3, characterized in that: The limiting member is two limiting rods (44) arranged parallel to the transmission rod (43). Correspondingly, the cutting knife (41) includes a cutting knife seat (40) connected to the transmission rod (43), and the cutting knife seat (40) is provided with through holes (401) for the two limiting rods (44) to pass through respectively.

5. The portable immunomicrofluidic detector according to any one of claims 1 to 4, characterized in that: The housing (11) is also provided with a syringe pump drive area (6), the syringe pump drive area (6) comprising a syringe pump cavity (61), a water inlet pipe (62) provided on the cavity (61) and connected to an outlet (25) of a microfluidic chip reaction area (2), and a water outlet pipe (63) provided on the cavity (61), wherein the liquid is discharged from the water outlet pipe (63) by the reciprocating motion of the piston in the syringe pump.

6. The portable immunomicrofluidic detector according to claim 5, characterized in that: The shell (11) is also provided with a waste liquid collection area (7) connected to the water outlet pipe (63), the waste liquid collection area (7) includes a waste liquid collection chamber (71), the shell (11) is provided with a first opening (14) corresponding to the waste liquid collection area (7), the first opening (14) can be used for the waste liquid collection chamber (71) of the waste liquid collection area (7) to be removably inserted, and correspondingly, the waste liquid collection chamber (71) is provided with a handle (72) exposed on the first opening (14).

7. The portable immunomicrofluidic detector according to claim 6, characterized in that: A micro vibrator (8) capable of enhancing mixing, as well as a photoelectric detector (9) and a photoelectric receiver (10) capable of detecting reaction concentration are also arranged in the housing (11) on the microfluidic chip reaction area (2).

8. The portable immunomicrofluidic detector according to claim 6 or 7, characterized in that: The housing (11) is also provided with buttons (30) capable of controlling the microfluidic chip reaction area (2), the injection pump driving area (6), the bagging mechanism (4) and the waste liquid collection area (7).

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