Disc type intermittent immersion plant bioreactor

Abstract

The utility model discloses a disc type intermittent immersion plant bioreactor, which relates to the field of plant tissue culture and comprises a box body, a cabin wall is arranged in the box body, the box body is divided into a culture cabin and a motor cabin which are mutually sealed, a bottom opening of the culture cabin is used for being externally connected with a water pipe and introducing or discharging a culture solution into or out of the culture cabin, and a culture disc is obliquely arranged in the culture cabin. The plants are uniformly arranged in the culture tray along the circumferential direction, so that at least one part of the plants is immersed in the culture solution; a motor is fixedly mounted in the motor cabin, and the shaft end of the motor penetrates through the cabin wall and then is connected with and drives the culture tray, so that each part of the plant is intermittently immersed into the culture solution. According to the utility model, the motor is utilized to drive the culture tray to uniformly rotate in the culture cabin (water receiving tray), so that each part of the plant is intermittently immersed in the culture solution, the whole reactor is driven and controlled only by the motor, the structure is simple, the control is efficient, and the requirements of most plants with medium and low immersion frequency and some plants with high immersion frequency can be met.

Description

Technical Field

[0001] The utility model relates to the field of plant tissue culture, in particular to a disc-type intermittent immersion plant bioreactor. Background Art

[0002] Plant tissue culture refers to the technology of isolating the required tissues, organs or cells, protoplasts, etc. from the plant body, inoculating them on a culture medium containing various nutrients and plant hormones under sterile conditions for culturing to obtain regenerated complete plants or produce other products with economic value.

[0003] At present, in the field of plant tissue culture, there are two simple culture methods that are most widely used and easy to manage and implement. The first is solid or semi-solid culture using agar as a support in a culture bottle. This culture method requires a large amount of agar and a large number of containers need to be handled during filling and cleaning, so it consumes a lot of labor, resulting in high production costs. The second is the liquid culture method. Although this culture method has the advantages of easy updating, no need to change containers, convenient filtration and sterilization, easy cleaning, convenient large container application, and reduced seedling transfer time, the disadvantages of liquid culture are also relatively concentrated, such as easy hypoxia, long-term immersion in liquid, easy vitrification of plant organs, sensitivity of plant organs to stirring force, and the need for complex equipment.

[0004] Chinese patent document (CN203333668U) discloses a combined intermittent submerged plant bioreactor, including a reaction chamber and a liquid storage chamber, wherein the reaction chamber and the liquid storage chamber are separated by a partition, and a connecting hole connecting the reaction chamber and the liquid storage chamber is provided on the partition, and a first time controller and a first air pump are provided on the reaction chamber, and the first air pump is sealed and connected to a first air inlet pipe of the reaction chamber, and a first air filter is provided on the first air inlet pipe, and a second time controller and a second air pump are provided on the liquid storage chamber, and the second air pump is sealed and connected to a second air inlet pipe of the liquid storage chamber, and a second air filter is provided on the second air inlet pipe.

[0005] However, this technical solution requires the installation of multiple time controllers and multiple air pumps, which is relatively complex to control and requires many purchased parts, resulting in high production and use costs of the reactor, which is not conducive to popularization and application. If any of the components fails, the reactor will fail, and the reliability is low, which needs to be improved. Utility Model Content

[0006] The utility model aims to overcome the shortcomings of the prior art and provide a disc-type intermittent immersion plant bioreactor, which can meet the needs of most low and medium immersion frequency plants and some high immersion frequency plants.

[0007] The purpose of the utility model is achieved through the following technical solutions: this disc-type intermittent immersion plant bioreactor comprises a box body, a bulkhead is arranged in the box body, and the box body is divided into a culture chamber and a motor chamber which are sealed with each other, the bottom opening of the culture chamber is used for connecting an external water pipe to introduce or discharge culture solution into the culture chamber, a culture disc is arranged obliquely in the culture chamber, and plants are evenly arranged in the culture disc along the circumferential direction so that at least a part of the plants is immersed in the culture solution; a motor is fixedly installed in the motor chamber, and the motor shaft end passes through the bulkhead and is connected to drive the culture disc so that various parts of the plant are intermittently immersed in the culture solution.

[0008] As a further technical solution, a water receiving tray is provided at the bottom of the culture chamber for containing the culture solution, and the water receiving tray is connected to the water pipe.

[0009] As a further technical solution, the bulkhead is an inclined surface or a curved surface.

[0010] As a further technical solution, the culture plate is formed by a plurality of arc surfaces with gradually increasing diameters connected in sequence along the axial direction, and a plurality of water-permeable holes are provided on the arc surfaces.

[0011] As a further technical solution, a scale line is provided on the side wall of the culture chamber to indicate the liquid level of the culture solution, and the immersion frequency scale is marked on the scale line.

[0012] As a further technical solution, the shaft end of the motor and the bulkhead are sealed by a sealing ring.

[0013] As a further technical solution, the maximum immersion frequency of the bioreactor is 1h / 5h.

[0014] The beneficial effects of the utility model are:

[0015] 1. The motor is used to drive the culture tray to rotate evenly in the culture chamber (water tray) to ensure that each part of the plant is intermittently immersed in the culture solution. The entire reactor only needs the motor for drive control, with a simple structure and efficient control, which effectively reduces production and use costs and is beneficial to promotion;

[0016] 2. Intermittent immersion culture reduces vitrification compared to liquid culture, making tissue culture seedlings healthier; sufficient gas exchange provides sufficient oxygen for the growth of tissue culture seedlings; nutrients are adequately mixed and fully utilized: limiting the shear force level; semi-automatic operation, and can easily and conveniently replace fresh culture medium to reduce the contamination rate;

[0017] 3. Scale lines are set on the side wall of the culture chamber. Since the immersion frequency depends on the proportion of the area of ​​the culture plate immersed in the culture solution, that is, it depends on the water level depth, the scale lines can indicate the liquid level and the immersion frequency at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of the utility model.

[0019] Figure 2 The structure of the culture tray in the utility model is shown in FIG. Figure 1 .

[0020] Figure 3 The structure of the culture tray in the utility model is shown in FIG. Figure 2 .

[0021] Explanation of the reference numerals: culture chamber 1, chamber wall 2, water receiving tray 3, culture tray 4, motor 5, water pipe 6, arc surface 7, water permeable hole 8, scale line 9, sealing ring 10, box body 11, motor chamber 12, culture solution 13. DETAILED DESCRIPTION

[0022] The utility model will be described in detail below with reference to the accompanying drawings:

[0023] Example: As attached Figures 1 to 3 As shown, the disc-type intermittent immersion plant bioreactor includes a culture chamber 1, a chamber wall 2, a water receiving tray 3, a culture tray 4, a motor 5, a water pipe 6, an arc surface 7, a water permeable hole 8, a scale line 9, a sealing ring 10, a box body 11, a motor chamber 12 and a culture solution 13.

[0024] Reference Figure 1 The bioreactor adopts a box-type structure, and a bulkhead 2 is arranged in the box body 11 (preferably, the bulkhead 2 is arranged as an inclined surface or an arc surface to avoid interference with the motor 5). The bulkhead 2 divides the box body 11 into two mutually sealed culture chambers 1 and a motor chamber 12, and the culture chamber 1 is located above the motor chamber 12. The bottom opening of the culture chamber 1 is used to connect an external water pipe 6, and the water pipe 6 is used to pass or discharge the culture liquid 13 into the culture chamber 1. As an optional technical solution, a water receiving tray 3 can be arranged at the bottom of the culture chamber 1 to accommodate the culture liquid 13, and the water receiving tray 3 is connected to the water pipe 6.

[0025] Furthermore, a culture tray 4 is tilted in the culture cabin 1, and the plants to be cultured are evenly arranged in the culture tray 4 along the circumferential direction. Since the culture tray 4 is tilted, at least a part of the plants is immersed in the culture solution 13. A motor 5 is fixedly installed in the motor cabin 12, and the shaft end of the motor 5 passes through the cabin wall 2 and is connected to the bottom of the culture tray 4, driving the culture tray 4 to rotate, so that various parts of the plants are intermittently immersed in the culture solution 13. Preferably, the shaft end of the motor 5 and the cabin wall 2 are sealed by a sealing ring 10 to prevent leakage of the culture solution 13.

[0026] like Figure 2 , 3As shown, the culture tray 4 is formed by a plurality of arc surfaces 7 with gradually increasing diameters connected in sequence along the axial direction, and a plurality of water-permeable holes 8 are provided on the arc surfaces 7 to facilitate the culture solution 13 to enter and exit the culture tray 4 .

[0027] As a preferred technical solution, Figure 1 As shown, a scale line 9 is set on the side wall of the culture chamber 1 to indicate the liquid level of the culture solution 13. Since the immersion frequency depends on the proportion of the area of ​​the culture tray 4 immersed in the culture solution 13, that is, it depends on the water level depth, the immersion frequency scale corresponding to the liquid level can be marked on the scale line 9 at the same time, so as to facilitate the adjustment of the appropriate liquid level according to different plants during use. In addition, the culture tray 4 is designed to be relatively flat, and the gravity effect is good during the plant cultivation process, which is conducive to its growth.

[0028] In this embodiment, the top opening diameter of the culture tray 4 is 40 cm, the culture tray 4 is arranged at an inclination angle of 24°, and the maximum immersion frequency of the bioreactor is 1 h / 5 h.

[0029] The working process of the utility model is as follows: when conducting plant tissue culture, the plants to be cultured are first evenly arranged in the culture tray 4 along the circumferential direction, and then the culture tray 4 is tilted and placed into the culture chamber 1, so that the shaft end of the motor 5 is sealed and connected with the culture tray 4. The height of the culture solution 13 to be added is determined according to the plants to be cultured, and the culture solution 13 is injected into the culture chamber 1 (water receiving tray 3) through the water pipe 6, and the scale line 9 is compared until the appropriate liquid level is reached. Start the motor 5 to drive the culture tray 4 to rotate, and the various parts of the plant can be intermittently immersed in the culture solution 13 according to the immersion frequency.

[0030] The advantage of the disc-type intermittent immersion plant bioreactor provided by the utility model is that it does not require a particularly complex control system. It only needs to control the motor speed to allow the plants therein to be immersed evenly and intermittently. Since the immersion frequency depends on the water level depth, an immersion frequency scale can be added to the water level scale on the inner wall of the culture chamber. In addition, since the culture disc is relatively flat, the gravity effect is good during the plant cultivation process, which is conducive to its growth. The immersion frequency of the disc-type intermittent immersion plant bioreactor is within a certain range, and the maximum immersion frequency is 1h / 5h, which can meet the needs of most medium and low immersion frequency plants and some high immersion frequency plants.

[0031] It is understandable that, for those skilled in the art, any equivalent replacement or change to the technical solution and the concept of the utility model should fall within the protection scope of the claims attached to the utility model.

Claims

1. A disc-type intermittent submerged plant bioreactor, characterized in that: The invention comprises a box body (11), wherein a bulkhead (2) is arranged inside the box body (11) to separate the box body (11) into a culture chamber (1) and a motor chamber (12) which are sealed from each other; the bottom opening of the culture chamber (1) is used to connect an external water pipe (6) to allow a culture solution (13) to be introduced into or discharged from the culture chamber (1); a culture tray (4) is arranged obliquely inside the culture chamber (1); plants are evenly arranged in the culture tray (4) along a circumferential direction so that at least a part of the plants is immersed in the culture solution (13); and a motor (5) is fixedly installed inside the motor chamber (12); the shaft end of the motor (5) passes through the bulkhead (2) and is connected to drive the culture tray (4) so ​​that various parts of the plants are intermittently immersed in the culture solution (13).

2. The disc-type intermittent submerged plant bioreactor according to claim 1, characterized in that: A water receiving tray (3) is arranged at the bottom of the culture chamber (1) for containing culture liquid (13), and the water receiving tray (3) is connected to a water pipe (6).

3. The disc-type intermittent submerged plant bioreactor according to claim 1, characterized in that: The bulkhead (2) is an inclined surface or a curved surface.

4. The disc-type intermittent submerged plant bioreactor according to claim 1, characterized in that: The culture plate (4) is formed by a plurality of arc surfaces (7) with gradually increasing diameters connected in sequence along the axial direction, and a plurality of water-permeable holes (8) are provided on the arc surfaces (7).

5. The disc-type intermittent submerged plant bioreactor according to claim 1, characterized in that: A scale line (9) is arranged on the side wall of the culture chamber (1) for indicating the liquid level of the culture liquid (13), and an immersion frequency scale is marked on the scale line (9).

6. The disc-type intermittent submerged plant bioreactor according to claim 1, characterized in that: The shaft end of the motor (5) and the bulkhead (2) are sealed via a sealing ring (10).

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