Rapid separation device for primary cell digestion
By designing a primary cell digestion and rapid separation device with a debugged tilt device, the problem of fixed rotation angle of the existing device is solved, and a more ideal stratification of cell precipitation fluid is achieved, which improves the practicality of separation.
Patent Information
- Application Number
- CN202421173180.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-27
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2034-05-27
AI Technical Summary
The rotation angle of the existing primary cell digestion rapid separation device cannot meet the ideal stratification needs of cell precipitate under different centrifugal conditions.
A device including a centrifugal box, a first motor, a centrifugal slot, a second motor, a threaded rod, a nut rack, a tooth plate and a half gear is designed. The threaded rod is driven to rotate through the second motor, adjust the inclination angle of the test tube, and pass the The first motor drives the centrifugal tank to rotate rapidly, achieving rapid separation of primary cells.
Different inclination angles can be set according to needs, the stratification of cell precipitate solution can be optimized, and different separation needs can be met, improving the practicality of separation.
Smart Images
Figure CN222428260U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of separation devices, in particular to a primary cell digestion and rapid separation device. Background Art
[0002] Primary cells refer to cells that are cultured immediately after being taken out of the body. The primary cell digestion culture method is a method for obtaining primary cells. It requires taking out tissue samples and placing tissue blocks in a digestion solution containing enzymes for digestion. After digestion, the digestion solution is centrifuged, the supernatant is removed, and the cell precipitate is left. This requires the use of a primary cell digestion rapid separation device;
[0003] During the centrifugation process, cells will be stratified and then precipitated according to their size, shape and density. The centrifugal angle, that is, the degree of inclination of the liquid during centrifugation, will affect the distribution of the layers and the aggregation pattern of the cells. Different inclination angles need to be set according to needs during centrifugation to obtain a more ideal cell precipitation liquid. Currently, some primary cell digestion rapid separation devices on the market have a fixed rotation angle and cannot meet the above requirements. Therefore, a primary cell digestion rapid separation device is proposed. Utility Model Content
[0004] The purpose of the utility model is to provide a primary cell digestion and rapid separation device to solve the problems raised in the above background technology.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a primary cell digestion and rapid separation device, comprising a centrifuge box, a first motor is fixedly connected to the lower surface of the inner wall of the centrifuge box, an output shaft end of the first motor is fixedly connected to a centrifugal groove, a second motor is fixedly connected to the lower surface of the inner wall of the centrifugal groove, a threaded rod is fixedly connected to the output shaft end of the second motor, a nut rack is threadedly connected to the outer wall of the threaded rod, two tooth plates are symmetrically welded to the outer wall of the nut rack, two rotating rods are rotatably arranged inside the centrifugal groove, both ends of the rotating rods are rotatably connected to support columns through bearings, the lower surface of the support column is fixedly connected to the lower surface of the inner wall of the centrifugal groove, the outer wall of the rotating rod is fixedly connected to a mounting seat, the upper surface of the mounting seat is fixedly connected to a clamping frame made of elastic material, the inner wall of the clamping frame is in close contact with a test tube, a half gear is welded to the outer wall of the rotating rod, and the half gear is meshed with the tooth plate.
[0006] As a further preferred embodiment of the present technical solution: a hinge is hingedly connected to the rear side wall of the centrifuge box, and a cover plate is hingedly connected to the front surface of the hinge.
[0007] As a further preferred embodiment of the present technical solution: a handle is welded on the upper surface of the cover plate.
[0008] As a further preferred embodiment of the technical solution: a sealing ring is bonded to the upper surface of the centrifuge box, and the lower surface of the cover plate is in movable contact with the upper surface of the sealing ring.
[0009] As a further preferred embodiment of the present technical solution: two guide rods are fixedly connected to the lower surface of the inner wall of the centrifugal groove, the outer wall of the guide rod is slidably connected to the nut rack, and the upper surface of the guide rod is fixedly connected to a limiting plate.
[0010] As a further preferred embodiment of the present technical solution: legs are uniformly welded on the lower surface of the centrifuge box.
[0011] As a further preferred embodiment of the present technical solution: a rubber stopper is threadedly connected to the upper surface of the test tube.
[0012] As a further preferred embodiment of the present technical solution: a first groove is fixedly connected to the lower surface of the centrifugal tank, a second groove is fixedly connected to the lower surface of the inner wall of the centrifugal box, and balls are evenly arranged between the first groove and the second groove.
[0013] Compared with the prior art, the beneficial effects of the utility model are:
[0014] The utility model starts the second motor to drive the threaded rod to rotate, drives the nut frame and the tooth plate to move, causes the half gear meshing with the tooth plate to rotate, and the half gear drives the rotating rod, the mounting seat and the clamping frame to rotate, thereby adjusting the inclination angle of the test tube, and then starts the first motor to drive the centrifugal tank to rotate quickly, thereby completing the rapid separation of primary cells after digestion. Different inclination angles can be set as needed to obtain a more ideal cell precipitation solution, so that the stratification of the cell precipitation solution better meets the needs of separation and is more practical. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a schematic diagram of the main structure of the utility model;
[0016] Figure 2 This is a schematic diagram of the internal structure of the centrifugal box in the utility model;
[0017] Figure 3 It is a structural schematic diagram of the threaded rod and the nut frame in the utility model;
[0018] Figure 4 This is a schematic diagram of the structure of the tooth plate and the half gear in the utility model;
[0019] Figure 5 It is a schematic cross-sectional structural diagram of the first motor and the centrifugal tank in the utility model.
[0020] In the figure: 1, centrifugal box; 2, first motor; 3, centrifugal tank; 4, second motor; 5, threaded rod; 6, nut rack; 7, tooth plate; 8, rotating rod; 9, supporting column; 10, mounting seat; 11, clamping frame; 12, test tube; 13, half gear; 14, hinge; 15, cover plate; 16, handle; 17, sealing ring; 18, guide rod; 19, limit plate; 20, support leg; 21, rubber plug; 22, first groove; 23, second groove; 24, ball. DETAILED DESCRIPTION
[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Examples of the embodiments are shown in the drawings, where the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present application, and cannot be understood as limitations on the present application. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.
[0022] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", and "set" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.
[0023] See also Figure 1-5The utility model provides a technical solution: a primary cell digestion and rapid separation device, comprising a centrifugal box 1, a first motor 2 is fixedly connected to the lower surface of the inner wall of the centrifugal box 1, a centrifugal tank 3 is fixedly connected to the output shaft end of the first motor 2, a second motor 4 is fixedly connected to the lower surface of the inner wall of the centrifugal tank 3, a threaded rod 5 is fixedly connected to the output shaft end of the second motor 4, a nut rack 6 is threadedly connected to the outer wall of the threaded rod 5, two tooth plates 7 are symmetrically welded to the outer wall of the nut rack 6, two rotating rods 8 are rotatably arranged inside the centrifugal tank 3, both ends of the rotating rod 8 are rotatably connected to support columns 9 through bearings, and the lower surface of the support column 9 is fixedly connected to the centrifugal tank 3. On the lower surface of the inner wall, the outer wall of the rotating rod 8 is fixedly connected with a mounting seat 10, and the upper surface of the mounting seat 10 is fixedly connected with a clamping frame 11 of elastic material, and the inner wall of the clamping frame 11 is in close contact with a test tube 12, and a half gear 13 is welded to the outer wall of the rotating rod 8, and the half gear 13 is meshed with the tooth plate 7; start the second motor 4 to drive the threaded rod 5 to rotate, drive the nut frame 6 and the tooth plate 7 to move, and rotate the half gear 13 meshed with the tooth plate 7, and the half gear 13 drives the rotating rod 8, the mounting seat 10 and the clamping frame 11 to rotate, so as to adjust the inclination angle of the test tube 12, and then start the first motor 2 to drive the centrifuge tank 3 to rotate rapidly, so as to complete the rapid separation after the primary cell digestion.
[0024] In this embodiment, specifically: a hinge 14 is hingedly connected to the rear side wall of the centrifugal box 1 , and a cover plate 15 is hingedly connected to the front surface of the hinge 14 ; this facilitates opening and closing of the centrifugal box 1 .
[0025] In this embodiment, specifically: a handle 16 is welded on the upper surface of the cover plate 15 to facilitate pulling the cover plate 15 .
[0026] In this embodiment, specifically: a sealing ring 17 is bonded to the upper surface of the centrifugal box 1 , and the lower surface of the cover plate 15 is in movable contact with the upper surface of the sealing ring 17 , so as to prevent dust from entering the interior of the centrifugal box 1 .
[0027] In this embodiment, specifically: two guide rods 18 are fixedly connected to the lower surface of the inner wall of the centrifugal groove 3, the outer wall of the guide rod 18 is slidably connected to the nut rack 6, and the upper surface of the guide rod 18 is fixedly connected to a limiting plate 19; so that the nut rack 6 does not rotate with the rotation of the threaded rod 5, but keeps moving in the direction of the guide rod 18.
[0028] In this embodiment, specifically: the lower surface of the centrifuge box 1 is evenly welded with supporting legs 20 for supporting the centrifuge box 1 .
[0029] In this embodiment, specifically: a rubber stopper 21 is threadedly connected to the upper surface of the test tube 12 to prevent the cell fluid in the test tube 12 from splashing out.
[0030] In this embodiment, specifically: a first groove 22 is fixedly connected to the lower surface of the centrifugal groove 3, a second groove 23 is fixedly connected to the lower surface of the inner wall of the centrifugal box 1, and balls 24 are evenly arranged between the first groove 22 and the second groove 23; so that the centrifugal groove 3 rotates more smoothly.
[0031] The working principle of the utility model is: starting the second motor 4 drives the threaded rod 5 to rotate, drives the nut frame 6 and the tooth plate 7 to move, and rotates the half gear 13 meshing with the tooth plate 7, and the half gear 13 drives the rotating rod 8, the mounting seat 10 and the clamping frame 11 to rotate, so as to adjust the inclination angle of the test tube 12, and then starts the first motor 2 to drive the centrifugal tank 3 to rotate rapidly, so as to complete the rapid separation after the primary cell digestion, thereby forming a primary cell digestion rapid separation device, and different inclination angles can be set as needed to obtain a more ideal cell precipitation solution, so that the stratification of the cell precipitation solution is more in line with the separation needs, and the practicality is stronger.
[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A primary cell digestion and rapid separation device, comprising a centrifuge box (1), characterized in that: The lower surface of the inner wall of the centrifugal box (1) is fixedly connected to a first motor (2), the output shaft end of the first motor (2) is fixedly connected to a centrifugal groove (3), the lower surface of the inner wall of the centrifugal groove (3) is fixedly connected to a second motor (4), the output shaft end of the second motor (4) is fixedly connected to a threaded rod (5), the outer wall of the threaded rod (5) is threadedly connected to a nut rack (6), the outer wall of the nut rack (6) is symmetrically welded with two tooth plates (7), the inner part of the centrifugal groove (3) is rotatably provided with two rotating rods (8), the Both ends of the rotating rod (8) are rotatably connected to support columns (9) via bearings; the lower surface of the support column (9) is fixedly connected to the lower surface of the inner wall of the centrifugal tank (3); the outer wall of the rotating rod (8) is fixedly connected to a mounting seat (10); the upper surface of the mounting seat (10) is fixedly connected to a clamping frame (11) made of elastic material; the inner wall of the clamping frame (11) is in close contact with a test tube (12); a half gear (13) is welded to the outer wall of the rotating rod (8); and the half gear (13) is meshingly connected to the toothed plate (7).
2. The primary cell digestion rapid separation device according to claim 1, characterized in that: A hinged member (14) is hingedly connected to the rear side wall of the centrifugal box (1), and a cover plate (15) is hingedly connected to the front surface of the hinged member (14).
3. The primary cell digestion and rapid separation device according to claim 2, characterized in that: A handle (16) is welded to the upper surface of the cover plate (15).
4. The primary cell digestion and rapid separation device according to claim 2, characterized in that: A sealing ring (17) is bonded to the upper surface of the centrifugal box (1), and the lower surface of the cover plate (15) is in movable contact with the upper surface of the sealing ring (17).
5. The primary cell digestion and rapid separation device according to claim 2, characterized in that: Two guide rods (18) are fixedly connected to the lower surface of the inner wall of the centrifugal groove (3), the outer wall of the guide rod (18) is slidably connected to the nut frame (6), and the upper surface of the guide rod (18) is fixedly connected to a limiting plate (19).
6. The primary cell digestion and rapid separation device according to claim 2, characterized in that: The lower surface of the centrifuge box (1) is evenly welded with supporting legs (20).
7. The primary cell digestion and rapid separation device according to claim 6, characterized in that: The upper surface of the test tube (12) is threadedly connected with a rubber stopper (21).
8. The primary cell digestion and rapid separation device according to claim 6, characterized in that: A first groove (22) is fixedly connected to the lower surface of the centrifugal tank (3), a second groove (23) is fixedly connected to the lower surface of the inner wall of the centrifugal box (1), and balls (24) are evenly arranged between the first groove (22) and the second groove (23).