Magnetic immobilized enzyme recycling device

Abstract

The utility model discloses a magnetic immobilized enzyme recycling device, which relates to the field of immobilized enzyme recovery and comprises a reaction tank, a filler screen is arranged on the reaction tank, and the aperture of the filler screen is larger than the diameter of immobilized enzyme; the device further comprises a collecting mechanism, and the collecting mechanism is located on the movement stroke of the immobilized enzyme. According to the magnetic immobilized enzyme recycling device provided by the utility model, the aperture of the filler screen is set to be larger than the diameter of the immobilized enzyme, so that the airflow interaction amount of the filler screen is increased, and the circulation efficiency of substances is accelerated; and an independent collecting mechanism is designed, so that the immobilized enzyme flowing out of the sieve pores can be independently collected, and cyclic utilization is realized.

Description

Technical Field

[0001] The utility model relates to the field of immobilized enzyme recovery, in particular to a magnetic immobilized enzyme recycling device. Background Art

[0002] As is known to all, immobilized enzymes refer to enzymes that catalyze within a certain spatial range and can be used repeatedly and continuously. Usually, enzyme catalysis reactions are carried out in aqueous solutions, while immobilized enzymes are water-soluble enzymes treated by physical or chemical methods to make them insoluble in water but still have enzyme activity.

[0003] For example, the patent document with the authorization announcement number CN220056862U, the authorization date 2023-11-21, and the name "A kind of immobilized enzyme screen reactor" includes a reaction tank; the beneficial effect of the utility model is that the solution in the reaction tank enters the separation device, the filter mesh of the debris screen is larger than the filter mesh of the immobilized enzyme screen, the precipitate in the solution is filtered and retained by the debris screen, and the immobilized enzyme is filtered and retained by the immobilized enzyme screen, and the filtered solution is discharged through the second water outlet pipe. After the solution in the reaction tank is discharged through the second water outlet pipe, the discharge port is opened by moving the baffle upward, and the spiral blade is driven by the output end of the motor to rotate on the surface of the debris screen and the immobilized enzyme screen, and the rotating spiral blade disperses the material on the screen to the surroundings, and the dispersed material around is discharged to the outside of the cylinder through the discharge port, and the personnel collect the immobilized enzyme discharged from the lower discharge port, which is convenient for the next use of the immobilized enzyme and saves costs.

[0004] The shortcomings of the above-mentioned prior art are that, in some gas-liquid interaction reaction systems, small-pore screens or membrane structures have great restrictions on the gas-liquid flow rate, that is, it is difficult to take into account both the interception effect of the screen or membrane structure and the larger gas-liquid flow rate. In layman's terms, when the pore size of the screen is very small, large-flow gas-liquid interaction cannot be carried out, thereby affecting the flow efficiency of the substance. Utility Model Content

[0005] The purpose of the utility model is to provide a magnetic immobilized enzyme recycling device to solve the above-mentioned shortcomings in the prior art.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A magnetic immobilized enzyme recycling device comprises a reaction tank, wherein a filler screen is arranged on the reaction tank, and the pore size of the filler screen is larger than the diameter of the immobilized enzyme;

[0008] It also includes a collecting mechanism, which is located on the movement path of the immobilized enzyme.

[0009] In the above-mentioned magnetic immobilized enzyme recycling device, the collecting mechanism comprises a collecting bin, and an electromagnet is arranged on the outer side wall of the collecting bin.

[0010] In the above-mentioned magnetic immobilized enzyme recycling device, a stirring mechanism for mixing the immobilized enzyme with the solution is arranged inside the collection bin.

[0011] In the above-mentioned magnetic immobilized enzyme recycling device, the stirring mechanism comprises a driving motor arranged on the collecting bin, and a stirring shaft is fixedly connected to the output end of the driving motor.

[0012] The magnetic immobilized enzyme recycling device also includes a conveying mechanism for conveying the mixed solution to the reaction tank again.

[0013] In the above-mentioned magnetic immobilized enzyme recycling device, the conveying mechanism comprises a conveying pipe, both ends of which are respectively connected to the collecting bin and the reaction tank, and a circulating pump is arranged on the conveying pipe.

[0014] In the above-mentioned magnetic immobilized enzyme recycling device, the reaction tank is provided with a feed pipe and an air inlet pipe, the collection bin is provided with a discharge pipe, and the discharge pipe is provided with a first valve.

[0015] The magnetic immobilized enzyme recycling device further comprises a liquid distributor, which is arranged between the conveying pipe and the filler screen.

[0016] The magnetic immobilized enzyme recycling device further comprises a gas distributor, and the gas distributor is located between the filler screen and the collection bin.

[0017] The magnetic immobilized enzyme recycling device mentioned above has a partition plate inside the collecting bin, which divides the collecting bin into a left chamber and a right chamber. A second valve is provided on the inlet of the left chamber and the right chamber, and a third valve is provided on the outlet of the left chamber and the right chamber.

[0018] In the above technical scheme, the utility model provides a magnetic immobilized enzyme recycling device, in which the aperture of the filler screen is set to be larger than the diameter of the immobilized enzyme, thereby increasing the airflow interaction of the filler screen, thereby accelerating the flow efficiency of the substance;

[0019] Moreover, a separate collecting mechanism is designed to separately collect the immobilized enzyme flowing out of the sieve holes to realize recycling. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0021] Figure 1 The present invention is a cross-sectional structural schematic diagram provided for another embodiment of the present invention.

[0022] Description of reference numerals:

[0023] 1. Reaction tank; 2. Filling screen; 3. Collection bin; 301. Left chamber; 302. Right chamber; 4. Electromagnet; 5. Drive motor; 6. Stirring shaft; 7. Delivery pipe; 8. Circulation pump; 9. Feed pipe; 10. Air inlet pipe; 11. Discharge pipe; 12. First valve; 13. Liquid distributor; 14. Gas distributor; 15. Partition; 16. Second valve; 17. Third valve. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0025] In the description of the present utility model, it is necessary to understand that Figure 1 The position of the liquid distributor 13 relative to the gas distributor 14 is above, and vice versa. The terms "center", "up", "down", "left", "right", "top", "bottom", "inside", "outside", "circumferential", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model.

[0026] Reference Figure 1 A magnetic immobilized enzyme recycling device provided by an embodiment of the utility model includes a reaction tank 1, on which a filler screen 2 is provided, and the pore size of the filler screen 2 is larger than the diameter of the immobilized enzyme; and also includes a collecting mechanism, which is located on the movement stroke of the immobilized enzyme.

[0027] Specifically, the reaction tank 1 is a cylinder or a square cylinder, and the hollow area inside it is the filler and gas-liquid interaction area. The filler screen 2 is arranged at the bottom end of the reaction tank 1. In order to make the immobilized enzyme recyclable, the aperture of the filler screen 2 is set to be smaller than the diameter of the immobilized enzyme (this method will reduce the amount of airflow interaction of the filler screen 2). During the reaction, the immobilized enzyme remains in the reaction tank 1 to achieve the collection and reuse of the immobilized enzyme. This is a prior art and will not be repeated. One of the core innovations of the utility model is that two filler screens 2 are provided, and the two filler screens 2 are respectively arranged at the top and bottom of the reactor. The aperture of the filler screen 2 is set to be larger than the diameter of the immobilized enzyme, and the collecting mechanism can be an existing structure such as a filter bag. The effect of such a setting is that the gas enters the gas-liquid interaction area from the bottom end of the reaction tank 1, and the liquid enters the gas-liquid interaction area from the top end of the reaction tank 1. The filler screen 2 can remove large impurities in the incoming material without affecting the passage of the solution and the immobilized enzyme. Since the mesh of the filler screen 2 is large, the airflow interaction amount is increased, thereby accelerating the circulation efficiency of the material. After the reaction is completed, the immobilized enzyme and the solution flow from the reaction tank 1 into the collecting mechanism, so that the immobilized enzyme flowing out of the sieve hole can be collected separately to achieve recycling.

[0028] Preferably, the collecting mechanism comprises a collecting bin 3, the outer wall of the collecting bin 3 is provided with an electromagnet 4. Specifically, the immobilized enzyme is a magnetic immobilized enzyme, the collecting bin 3 is an internally hollow barrel having a liquid inlet and a liquid outlet, and a plurality of electromagnets 4 are provided and are evenly arranged on the outer wall of the collecting bin 3. The purpose of such a setting is that when the magnetic immobilized enzyme in the solution is collected, the electromagnet 4 is energized, and the magnetic immobilized enzyme in the solution will be adsorbed on the inner wall of the collecting bin 3 under the action of magnetic force, so as to realize the uniform collection of the magnetic immobilized enzyme, and the solution after the reaction is discharged from the liquid outlet.

[0029] Furthermore, a stirring mechanism for mixing the magnetic immobilized enzyme with the solution is provided inside the collecting bin 3; the stirring mechanism comprises a driving motor 5 provided on the collecting bin 3, a stirring shaft 6 is fixedly connected to the output end of the driving motor 5, specifically, the driving motor 5 is fixedly connected to the outer wall of the collecting bin 3, the stirring shaft 6 penetrates the side wall of the collecting bin 3 and extends to the inside thereof, and a plurality of stirring rods are evenly arranged on the outer peripheral surface of the stirring shaft 6, the purpose of such a setting is that, in order to facilitate the removal of the magnetic immobilized enzyme from the side wall of the collecting bin 3, the liquid outlet and the liquid inlet of the collecting bin 3 are closed, and at the same time, the electromagnet 4 is powered off, and the magnetic immobilized enzyme will fall from the side wall of the collecting bin 3 into the solution, at which time the collecting bin 3 contains a mixed solution of the magnetic immobilized enzyme, and at this time the driving motor 5 is started, and the driving motor 5 drives the stirring shaft 6 and the stirring rod at its output end to rotate, so that the magnetic immobilized enzyme can be evenly distributed in the solution, and after the stirring is completed, the mixed solution is discharged from the liquid outlet to obtain a mixed solution of the magnetic immobilized enzyme, thereby realizing the recovery of the magnetic immobilized enzyme.

[0030] Furthermore, it also includes a conveying mechanism for conveying the mixed solution to the reaction tank 1 again, the conveying mechanism includes a conveying pipe 7, the two ends of the conveying pipe 7 are respectively connected to the collection bin 3 and the reaction tank 1, and a circulation pump 8 is arranged on the conveying pipe 7. Specifically, one end of the conveying pipe 7 is connected to the bottom of the collection bin 3, and the other end is connected to the top of the reaction tank 1. The purpose of such arrangement is that after the stirring mechanism fully stirs the magnetic immobilized enzyme, the liquid outlet of the collection bin 3 is opened, and then the circulation pump 8 is turned on. The circulation pump 8 conveys the magnetic immobilized enzyme solution inside the collection bin 3 to the inside of the reaction tank 1 again to realize the recycling of the magnetic immobilized enzyme. After the conveying is completed, the electromagnet 4 is turned on again, and the stirring mechanism is turned off to prepare for the next collection of the magnetic immobilized enzyme.

[0031] Furthermore, the reaction tank 1 is provided with a feed pipe 9 and an air inlet pipe 10, the collecting bin 3 is provided with a discharge pipe 11, and the discharge pipe 11 is provided with a first valve 12. Specifically, the discharge pipe 11 is provided at the top of the reaction tank 1, and the feed pipe 9 is staggered with the liquid inlet of the reaction tank 1, and the discharge pipe 11 is provided at the bottom of the collecting bin 3, and is staggered with the liquid outlet of the collecting bin 3. The first valve 12 is preferably a spherical valve. The effect of such a setting is that during the reaction process, the liquid can be discharged through The feed pipe 9 enters into the reaction tank 1, and the gas can enter into the reaction tank 1 through the air inlet pipe 10. The added gas reacts with the liquid in the reaction tank 1, and the first valve 12 on the discharge pipe 11 is opened simultaneously, and the waste liquid after the reaction flows out from the discharge pipe 11 to avoid excessive solution accumulation in the reaction tank 1 so that the reaction process can continue. In the process of taking out the magnetic immobilized enzyme from the collection bin 3, the first valve 12 is closed to prevent the mixed solution containing the magnetic immobilized enzyme from flowing out of the discharge pipe 11.

[0032] Furthermore, it also includes a liquid distributor 13, which is arranged between the delivery pipe 7 and the packing screen 2. Specifically, the liquid distributor 13 is used to divide the liquid into multiple streams at the top of the packing screen 2 so that the liquid is evenly distributed inside the reaction tank 1, thereby making the liquid and the gas contact more fully.

[0033] Furthermore, it also includes a gas distributor 14, which is located between the packing screen 2 and the collecting bin 3. Specifically, the gas distributor 14 is a device that evenly distributes the introduced gas over the entire cross section, so that the gas is evenly distributed inside the reaction tank 1, thereby making the gas and liquid contact more fully.

[0034] As another embodiment of the utility model, a partition 15 is provided inside the collecting bin 3, and the partition 15 divides the collecting bin 3 into a left bin 301 and a right bin 302. A second valve 16 is provided on the inlet of the left bin 301 and the right bin 302, and a third valve 17 is provided on the outlet of the left bin 301 and the right bin 302. Specifically, two stirring mechanisms and two conveying mechanisms are provided, and the two stirring mechanisms are arranged corresponding to the left bin 301 and the right bin 302, respectively. The two conveying mechanisms are also arranged corresponding to the left bin 301 and the right bin 302, respectively. 2 is connected to the packing screen 2 at the bottom 1 end of the reaction tank through a pipeline, and the second valve 16 and the third valve 17 are both electric valves. The purpose of such a setting is that when the device is not running, all the electromagnets 4, the stirring mechanism and the conveying mechanism are in a closed state. When the device starts to run, the second valve 16 and the third valve 17 of the left chamber 301 are opened (the operation sequence of the left chamber 301 and the right chamber 302 is not required, that is, the second valve 16 and the third valve 17 of the right chamber 302 can also be opened first), the second valve 16 and the third valve 17 of the right chamber 302 are closed, the conveying mechanisms on the left and right sides remain closed, and the electromagnet 4 on the left side is turned on. The left chamber 301 is opened, the electromagnet 4 on the right side remains closed, the stirring mechanisms on the left and right sides remain closed, the liquid containing the magnetic immobilized enzyme in the gas-liquid interaction area flows into the left chamber 301, the magnetic immobilized enzyme in the liquid flow is adsorbed on the inner wall of the left chamber 301, and the remaining liquid flows into the downstream of the device through the discharge pipe 11. When the magnetic immobilized enzyme adsorbed on the inner wall of the left chamber 301 is too much, the second valve 16 and the third valve 17 on the left chamber 301 are closed, the second valve 16 and the third valve 17 on the right chamber 302 are opened, the conveying mechanism on the left side is opened, and the conveying mechanism on the right side remains closed, the stirring mechanism on the left side is opened, and the stirring mechanism on the right side remains closed, The electromagnet 4 on the left is automatically closed, and the electromagnet 4 on the right is automatically opened, and the magnetic immobilized enzyme on the inner wall of the left chamber 301 falls off and is mixed in the solution under the action of the stirring mechanism, and then is transported to the reaction tank 1 by the conveying mechanism on the left. The process occurring in the right chamber 302 is the same as the process of the previous step of the left chamber 301, that is, the liquid containing the magnetic immobilized enzyme in the reaction tank 1 flows into the right chamber 302, and the magnetic immobilized enzyme in the liquid flow is adsorbed on the inner wall of the right chamber 302, and the remaining liquid flows into the downstream of the device, and so on. The left chamber 301 and the right chamber 302 are alternately adsorbed, and the recycling of the magnetic immobilized enzyme can be realized without stopping the machine.

[0035] The above only describes some exemplary embodiments of the present invention by way of illustration. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A magnetic immobilized enzyme recycling device, comprising a reaction tank, characterized in that: The reaction tank is provided with a filler screen, and the pore size of the filler screen is larger than the diameter of the immobilized enzyme; It also includes a collecting mechanism, which is located on the movement path of the immobilized enzyme; The collecting mechanism comprises a collecting bin, and an outer side wall of the collecting bin is provided with an electromagnet.

2. A magnetic immobilized enzyme recycling device according to claim 1, characterized in that: The collection chamber is provided with a stirring mechanism for mixing the immobilized enzyme with the solution.

3. A magnetic immobilized enzyme recycling device according to claim 2, characterized in that: The stirring mechanism comprises a driving motor arranged on the collecting bin, and a stirring shaft is fixedly connected to an output end of the driving motor.

4. A magnetic immobilized enzyme recycling device according to claim 1, characterized in that: It also includes a conveying mechanism for conveying the mixed solution back into the reaction tank.

5. A magnetic immobilized enzyme recycling device according to claim 4, characterized in that: The conveying mechanism comprises a conveying pipe, two ends of which are respectively connected to the collecting bin and the reaction tank, and a circulating pump is arranged on the conveying pipe.

6. The magnetic immobilized enzyme recycling device according to claim 1, characterized in that: The reaction tank is provided with a feed pipe and an air inlet pipe, the collecting bin is provided with a discharge pipe, and the discharge pipe is provided with a first valve.

7. The magnetic immobilized enzyme recycling device according to claim 5, characterized in that: It also includes a liquid distributor, which is arranged between the conveying pipe and the filler screen.

8. The magnetic immobilized enzyme recycling device according to claim 5, characterized in that: It also includes a gas distributor, which is located between the filler screen and the collection bin.

9. The magnetic immobilized enzyme recycling device according to claim 1, characterized in that: A partition is provided inside the collecting bin, and the partition divides the collecting bin into a left chamber and a right chamber. A second valve is provided on the inlet of the left chamber and the right chamber, and a third valve is provided on the outlet of the left chamber and the right chamber.

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