Optimized cell exosome culture system

Abstract

The utility model relates to the technical field of cell exosome culture, in particular to a cell exosome optimized culture system which comprises a culture box, a placing groove is formed in the culture box, a culture box is placed in the placing groove, a sealing cover covers the outer side of a groove opening of the placing groove, a sealing ring is pasted on the edge of the inner side of the sealing cover, and the sealing ring is connected with the culture box. First connecting holes are formed in the side surface of the incubator and are positioned at four corners of the outer side of the placing groove. The cell exosome culture box has the beneficial effects that the cell exosome in the culture box can be optimally cultured, the culture box can be conveniently taken out through the handle, the sealing cover and the connecting rod, the culture box is clean and sanitary, dust and bacteria are reduced, and the culture box is convenient to disassemble, replace and clean; the placing position of the culture box is subjected to air exchange and air pressure balance through the air exchange hole and the air exchange machine, the culture box is kept in a good culture environment, and cell exosome culture is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of cell exosome culture, and in particular to a cell exosome optimization culture system. Background Art

[0002] Exosomes are tiny membrane-bound vesicles (40 to 200 nm in diameter) that are released extracellularly by many different types of cells under physiological and pathological conditions. Exosomes also contain signaling proteins, microRNA, mRNA, DNA and lipids, which can regulate various life activities of recipient cells during transport.

[0003] Exosomes can be defined as dynamic biological components of cells. In addition to intercellular communication and transcellular transport of molecular cargo, exosomes are also involved in a series of functions to maintain intracellular homeostasis, including affecting tumor occurrence by affecting the tumor microenvironment, angiogenesis, metastasis and immune system regulation.

[0004] In order to improve the research on exosomes, people place the exosome mother solution in a culture device for culture.

[0005] For example, the prior art Chinese patent publication number "CN110423686A" provides a cell exosome optimization culture system and a cell exosome optimization culture method, including: a cell-carrying component, a PDMS deformable membrane and a transparent cover plate, the cell-carrying component includes a microfluidic chip body formed by PDMS soft etching and irreversible sealing, and a transparent glass support plate bonded to the bottom end surface of the microfluidic chip body; wherein a plurality of array-distributed microfluidic channels that are interconnected are prefabricated in the microfluidic chip body; the PDMS deformable membrane is connected to the top end surface of the microfluidic chip body that is away from the transparent glass support plate; the PDMS deformable membrane is suitable for convexly deforming toward the side away from the transparent glass support plate; and a plurality of array-distributed shading strips are prefabricated on the transparent cover plate.

[0006] The device significantly improves the yield and biological quality of exosomes secreted by cells cultured in a 3D culture environment with stress stimulation.

[0007] In the existing cell exosome optimization culture system, the culture box used for cell exosome optimization culture is not convenient to take out after it is placed, and it is easy to bring in dust and bacteria on the hands, which is not hygienic and inconvenient to take and place the culture box and cell exosomes. At the same time, during the water bath heating of the culture box and the cell exosomes inside, the air pressure in the culture box will increase, and ventilation cannot be performed to balance the air pressure. Utility Model Content

[0008] The purpose of the utility model is to solve the shortcomings of the prior art that the culture box for optimizing the culture of extracellular vesicles is inconvenient to take out after being placed, and it is easy to bring in dust and bacteria on the hands, which is not hygienic and inconvenient to take and place the culture box and extracellular vesicles, and to propose an optimized culture system for extracellular vesicles.

[0009] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0010] A cell exosome optimization culture system is designed, including a culture box, a placement groove is opened inside the culture box, a culture box is placed inside the placement groove, a sealing cover is covered on the outer side of the groove of the placement groove, a sealing ring is adhered to the inner edge of the sealing cover, a first connecting hole is opened on the side of the culture box and located at the four corners of the outer side of the placement groove, a second connecting hole is opened at the four corners of the sealing cover, a bolt passes through the inside of the second connecting hole, the inner end of the bolt is threadedly connected to the first connecting hole, a third connecting hole is opened on the outer surface of the sealing cover, a connecting rod is fixed on the outer side of the culture box, the connecting rod passes through the third connecting hole, nuts are connected to the surface of the connecting rod and located at both ends of the third connecting hole, and a handle is fixed in the middle of the outer side of the sealing cover.

[0011] Furthermore, a temperature sensor is embedded in the inner bottom end of the placement groove, a heat-conducting copper column is embedded in the bottom end of the incubator, a water bath heating box is placed at the bottom end of the incubator, an electric heating rod is installed inside the water bath heating box, a water pipe is connected to the top end of the side of the water bath heating box, and a first support plate is fixed to the bottom end of the incubator.

[0012] Furthermore, a ventilation hole is opened at the top of the placement groove, and the ventilation hole passes through the top of the incubator. A ventilator is installed at the top of the incubator, and a second support plate is fixed to the top of the incubator.

[0013] Furthermore, the placement slot is a rectangular slot structure and is open, the sealing cover is detachably fastened and installed with the side of the incubator via the bolts, and the sealing ring is pressed against the outside of the slot of the placement slot to seal the placement slot.

[0014] Furthermore, the first connecting hole is a threaded hole structure and is aligned with the second connecting hole, and the sealing ring is a rectangular ring structure.

[0015] Furthermore, the connecting rod is a threaded rod structure, and is horizontally arranged, and is detachably fastened and installed with the sealing cover through the nut.

[0016] Furthermore, the temperature sensor is located at the bottom end of the culture box, the heat-conducting copper column is in contact with the top end of the water bath heating box, and the first support plate is located at both ends of the water bath heating box.

[0017] Furthermore, the ventilator is communicated with the ventilation hole, and the second support plate is located at both ends of the ventilator.

[0018] The utility model proposes an optimized culture system for exosomes, which has the following beneficial effects:

[0019] 1. The utility model sets two placement grooves which are not communicated with each other inside the incubator, and the culture box is installed on the inner side of the closed cover through a connecting rod. Thus, when the culture box is placed into the placement groove for cell exosome culture, the culture box is pushed into the placement groove through the closed cover, and the closed cover is set at the outer notch of the placement groove with a sealing ring and is fastened and installed by bolts. Therefore, the cell exosomes inside the culture box can be kept for optimized culture. Moreover, it is convenient and clean to take out the culture box through the handle, the closed cover and the connecting rod, which reduces the introduction of dust and bacteria, and is also convenient to disassemble, replace and clean the culture box.

[0020] 2. The utility model sets a water bath heating box at the bottom of the incubator, heats the culture box inside the incubator in a water bath through a heat-conducting copper column, and ventilates and balances the air pressure at the placement of the culture box through ventilation holes and a ventilator, thereby keeping the culture box in a good culture environment, which is conducive to the culture of cell exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0022] Figure 2 For this utility model Figure 1 A front view of

[0023] Figure 3 For this utility model Figure 1 A cross-sectional view of

[0024] Figure 4 For this utility model Figure 3 Enlarged view of point A.

[0025] In the figure: 1. incubator; 2. ventilator; 3. ventilation hole; 4. placement slot; 5. second support plate; 6. airtight cover; 7. handle; 8. first support plate; 9. water bath heating box; 10. electric heating rod; 11. heat-conducting copper column; 12. water pipe; 13. temperature sensor; 14. incubator box; 15. connecting rod; 16. nut; 17. third connecting hole; 18. bolt; 19. second connecting hole; 20. sealing ring; 21. first connecting hole. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0027] Example 1

[0028] See also Figure 1 , Figure 2 , Figure 3 and Figure 4 , a cell exosome optimization culture system shown in the figure includes a culture box 1, a placement groove 4 is opened inside the culture box 1, a culture box 14 is placed inside the placement groove 4, a sealing cover 6 is covered on the outside of the groove of the placement groove 4, a sealing ring 20 is adhered to the inner edge of the sealing cover 6, a first connecting hole 21 is opened on the side of the culture box 1 and located at the four corners of the outer side of the placement groove 4, a second connecting hole 19 is opened at the four corners of the sealing cover 6, a bolt 18 passes through the inside of the second connecting hole 19, and the inner end of the bolt 18 is threadedly connected with the first connecting hole 21, a third connecting hole 17 is opened on the outer surface of the sealing cover 6, a connecting rod 15 is fixed on the outer side of the culture box 14, the connecting rod 15 passes through the third connecting hole 17, nuts 16 are connected to the surface of the connecting rod 15 and located at both ends of the third connecting hole 17, and a handle 7 is fixed in the middle of the outer side of the sealing cover 6, and the handle 7 is an L-shaped structure, which is convenient for pushing and taking out the culture box 14.

[0029] When placing the culture box 14 into the placement groove 4 for cell extraosome culture, the culture box 14 is pushed into the placement groove 4 through the sealed cover 6. The sealed cover 6 is arranged at the outer notch of the placement groove 4 with a sealing ring 20 and is fastened and installed by bolts 18. Therefore, the cell extraosomes inside the culture box 14 can be kept for optimized culture. Moreover, it is convenient and clean to take out the culture box 14 through the handle 7, the sealed cover 6 and the connecting rod 15, which reduces the introduction of dust and bacteria and is also convenient for disassembly, replacement and cleaning of the culture box 14.

[0030] A temperature sensor 13 is embedded in the inner bottom end of the placement groove 4, a heat-conducting copper column 11 is embedded in the bottom end of the incubator 1, a water bath heating box 9 is placed at the bottom end of the incubator 1, an electric heating rod 10 is installed inside the water bath heating box 9, a water pipe 12 is connected to the top end of the side of the water bath heating box 9, and a first support plate 8 is fixed to the bottom end of the incubator 1.

[0031] A water bath heating box 9 is arranged at the bottom of the incubator 1 , and a culture box 14 inside the incubator 1 is heated by a water bath through a heat-conducting copper column 11 .

[0032] The placement slot 4 is a rectangular slot structure and is open. The sealed cover 6 is detachably fastened and installed with the side of the incubator 1 through the bolts 18, and the sealing ring 20 is pressed against the outside of the slot of the placement slot 4 to seal the placement slot 4.

[0033] The first connecting hole 21 is a threaded hole structure and is aligned with the second connecting hole 19. The sealing ring 20 is a rectangular ring structure. The connecting rod 15 is a threaded rod structure and is horizontally arranged and detachably fastened and installed with the sealing cover 6 through the nut 16.

[0034] The temperature sensor 13 is located at the bottom end of the culture box 14 , the heat-conducting copper column 11 is in contact with the top end of the water bath heating box 9 , and the first support plate 8 is located at both ends of the water bath heating box 9 .

[0035] Example 2

[0036] See also Figure 1 , Figure 2 , Figure 3 and Figure 4 , a cell exosome optimization culture system shown in the figure includes a culture box 1, a placement groove 4 is opened inside the culture box 1, a culture box 14 is placed inside the placement groove 4, a sealing cover 6 is covered on the outside of the groove of the placement groove 4, a sealing ring 20 is adhered to the inner edge of the sealing cover 6, a first connecting hole 21 is opened on the side of the culture box 1 and located at the four corners of the outer side of the placement groove 4, a second connecting hole 19 is opened at the four corners of the sealing cover 6, a bolt 18 passes through the inside of the second connecting hole 19, and the inner end of the bolt 18 is threadedly connected with the first connecting hole 21, a third connecting hole 17 is opened on the outer surface of the sealing cover 6, a connecting rod 15 is fixed on the outer side of the culture box 14, the connecting rod 15 passes through the third connecting hole 17, nuts 16 are connected to the surface of the connecting rod 15 and located at both ends of the third connecting hole 17, and a handle 7 is fixed in the middle of the outer side of the sealing cover 6, and the handle 7 is an L-shaped structure, which is convenient for pushing and taking out the culture box 14.

[0037] When placing the culture box 14 into the placement groove 4 for cell extraosome culture, the culture box 14 is pushed into the placement groove 4 through the sealed cover 6. The sealed cover 6 is arranged at the outer notch of the placement groove 4 with a sealing ring 20 and is fastened and installed by bolts 18. Therefore, the cell extraosomes inside the culture box 14 can be kept for optimized culture. Moreover, it is convenient and clean to take out the culture box 14 through the handle 7, the sealed cover 6 and the connecting rod 15, which reduces the introduction of dust and bacteria and is also convenient for disassembly, replacement and cleaning of the culture box 14.

[0038] A temperature sensor 13 is embedded in the inner bottom end of the placement groove 4, a heat-conducting copper column 11 is embedded in the bottom end of the incubator 1, a water bath heating box 9 is placed at the bottom end of the incubator 1, an electric heating rod 10 is installed inside the water bath heating box 9, a water pipe 12 is connected to the top end of the side of the water bath heating box 9, and a first support plate 8 is fixed to the bottom end of the incubator 1.

[0039] A ventilation hole 3 is provided at the top of the placement slot 4, and the ventilation hole 3 passes through the top of the incubator 1. A ventilator 2 is installed at the top of the incubator 1, and a second support plate 5 is fixed to the top of the incubator 1; the ventilator 2 is connected to the ventilation hole 3, and the second support plate 5 is located at both ends of the ventilator 2.

[0040] A water bath heating box 9 is provided at the bottom of the incubator 1, and the culture box 14 inside the incubator 1 is heated by a water bath through a heat-conducting copper column 11, and the ventilation holes 3 and the ventilator 2 are used to ventilate and balance the air pressure at the placement of the culture box 14, so as to keep the culture box 14 in a good culture environment, which is conducive to the culture of cell exosomes.

[0041] Working method: by setting two placement grooves 4 that are not connected to each other inside the incubator 1, and the culture box 14 is installed on the inner side of the closed cover 6 through the connecting rod 15, so that when the culture box 14 is placed in the placement groove 4 for cell extracellular vesicle culture, the culture box 14 is pushed into the placement groove 4 through the closed cover 6, and the closed cover 6 is padded with a sealing ring 20 and is set at the outer notch of the placement groove 4, and is fastened and installed by bolts 18, so that the cell extracellular vesicles inside the culture box 14 can be kept for optimized culture, and it is convenient to take out the culture box 14 through the handle 7, the closed cover 6 and the connecting rod 15, which is clean and hygienic, reduces the introduction of dust and bacteria, and is also convenient to disassemble, replace and clean the culture box 14.

[0042] A water bath heating box 9 is provided at the bottom of the incubator 1, and the culture box 14 inside the incubator 1 is heated by a water bath through a heat-conducting copper column 11, and the ventilation holes 3 and the ventilator 2 are used to ventilate and balance the air pressure at the placement of the culture box 14, so as to keep the culture box 14 in a good culture environment, which is conducive to the culture of cell exosomes.

[0043] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell exosome optimization culture system, comprising an incubator (1), characterized in that: The culture box (1) has a placement groove (4) formed inside, a culture box (14) is placed inside the placement groove (4), a sealing cover (6) is provided on the outside of the groove opening of the placement groove (4), a sealing ring (20) is adhered to the inner edge of the sealing cover (6), a first connection hole (21) is formed on the side of the culture box (1) and at the four corners outside the placement groove (4), second connection holes (19) are formed at the four corners of the sealing cover (6), and the inside of the second connection hole (19) passes through a A bolt (18), the inner end of the bolt (18) is threadedly connected to the first connecting hole (21), a third connecting hole (17) is opened on the outer surface of the sealed cover (6), a connecting rod (15) is fixed to the outer side of the culture box (14), the connecting rod (15) passes through the third connecting hole (17), nuts (16) are connected to the surface of the connecting rod (15) and located at both ends of the third connecting hole (17), and a handle (7) is fixed in the middle of the outer side of the sealed cover (6).

2. The cell exosome optimization culture system according to claim 1, characterized in that: A temperature sensor (13) is embedded in the bottom of the placement groove (4), a heat-conducting copper column (11) is embedded in the bottom of the incubator (1), a water bath heating box (9) is placed at the bottom of the incubator (1), an electric heating rod (10) is installed inside the water bath heating box (9), a water pipe (12) is connected to the top of the side of the water bath heating box (9), and a first support plate (8) is fixed to the bottom of the incubator (1).

3. The cell exosome optimization culture system according to claim 1, characterized in that: A ventilation hole (3) is provided at the top of the placement groove (4), and the ventilation hole (3) passes through the top of the incubator (1). A ventilator (2) is installed at the top of the incubator (1), and a second support plate (5) is fixed at the top of the incubator (1).

4. The cell exosome optimization culture system according to claim 1, characterized in that: The placement groove (4) is a rectangular groove structure and is open. The sealing cover (6) is detachably fastened and installed with the side of the incubator (1) via the bolts (18), and the sealing ring (20) is pressed against the outside of the groove opening of the placement groove (4), thereby sealing the placement groove (4).

5. The cell exosome optimization culture system according to claim 1, characterized in that: The first connection hole (21) is a threaded hole structure and is aligned with the second connection hole (19); the sealing ring (20) is a rectangular ring structure.

6. The cell exosome optimization culture system according to claim 1, characterized in that: The connecting rod (15) is a threaded rod structure and is arranged horizontally, and is detachably fastened and installed with the sealing cover (6) via the nut (16).

7. The cell exosome optimization culture system according to claim 2, characterized in that: The temperature sensor (13) is located at the bottom end of the culture box (14), the heat-conducting copper column (11) is in contact with the top end of the water bath heating box (9), and the first support plate (8) is located at both ends of the water bath heating box (9).

8. The cell exosome optimization culture system according to claim 3, characterized in that: The ventilator (2) is in communication with the ventilating hole (3), and the second supporting plate (5) is located at both ends of the ventilator (2).

Images