Fermentation device special for lactic acid bacteria

Abstract

The utility model discloses a special fermentation device for lactic acid bacteria. The device is mainly divided into three parts, namely a fermentation tank body, an ozone sterilization device and an external circulation ultraviolet sterilization device, a tank cover is mounted at a top opening of the fermentation tank body, tank legs are arranged on the periphery of the bottom of the fermentation tank, a discharge port communicated with the inside and the outside of the fermentation tank is formed in the center of the bottom of the fermentation tank, and the fermentation tank is provided with a pH detection device and a temperature control device. According to the metabolic characteristics of lactic acid bacteria, equipment of the fermentation tank is optimized, a stirring device of the fermentation tank is reduced, the mass transfer effect is achieved through the external circulating pump, and high-temperature and high-pressure steam sterilization is replaced by a mode of combining ozone sterilization and ultraviolet sterilization; therefore, the equipment is further utilized to achieve the effect of uniform fermentation liquor while sterilization is achieved, the fermentation cost is reduced, the safety of the fermentation process is improved, and the method is generally suitable for lactobacillus fermentation.

Description

Technical Field

[0001] The utility model relates to the technical field of fermentation equipment, in particular to a special fermentation device for lactic acid bacteria. Background Art

[0002] Lactic acid bacteria is a general term for bacteria that can produce large amounts of lactic acid using fermentable carbohydrates. They are non-spore-forming, Gram-positive bacteria, and most lactic acid bacteria are aerobic and facultative anaerobes. Lactic acid bacteria have a strong ability to produce acid and resist acid. They continuously produce lactic acid, making the environment acidic and inhibiting a variety of acid-intolerant bacteria. The culture medium for lactic acid bacteria is mostly sterilized by steam in a fermentation tank, but steam sterilization will relatively damage the components of the culture medium, and steam sterilization is relatively dangerous, and operators need professional knowledge. In addition, general bacterial fermentation requires stirring to achieve mass transfer of materials, oxygen, etc. to meet the needs, while lactic acid bacteria grow better without stirring conditions and are more suitable for achieving high-density fermentation of lactic acid bacteria.

[0003] Fermentation tank refers to the device used for microbial fermentation in industry. As a common factory equipment, its main body is generally a double-layer main cylinder made of stainless steel plate. Steam sterilization is generally used for sterilization, which uses high-pressure saturated steam to denature the protein and nucleic acid in the microbial cells to kill the microorganisms. Fermentation tanks are generally equipped with steam generators, air filters and other equipment, and operators are required to have special operation certificates. Therefore, what kind of sterilization method can achieve the sterilization effect and be safe and easy to operate has become the key development direction of fermentation equipment.

[0004] Ozone sterilization uses ozone dissolved in water to decompose highly oxidizing oxygen ions, which oxidize and decompose the enzymes needed to degrade glucose inside bacteria, making the TCA cycle unable to proceed, resulting in the inability to supply ATP required for cell life activities, inactivating and killing bacteria, and achieving the purpose of sterilization. The half-life of ozone molecules at room temperature is about 16 minutes. After the sterilization is completed, the residual ozone in the culture medium will not cause harm to the seed bacteria. Ultraviolet sterilization is the most destructive to the molecular structure of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) in bacteria and viruses with ultraviolet wavelengths in the range of 240 to 280nm, thereby causing growth cell death and (or) regenerative cell death to achieve the purpose of sterilization, and the penetration of ultraviolet rays is not strong, so it can be controlled within a certain range. The above two sterilization methods are safer and more energy-efficient than steam sterilization. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide a lactic acid bacteria special fermentation device in view of the deficiencies of the prior art.

[0006] In order to solve the above technical problems, the utility model discloses a lactic acid bacteria special fermentation device, providing the following technical solutions:

[0007] A lactic acid bacteria fermentation device comprises: a fermentation tank body and an external circulation ultraviolet sterilization device; the external circulation ultraviolet sterilization device comprises an ultraviolet circulation pipeline connected to the fermentation tank body and an ultraviolet lamp located on one side of the ultraviolet circulation pipeline. Preferably, the lactic acid bacteria fermentation device has a size and shape suitable for the lactic acid bacteria fermentation process; the fermentation tank is made of food-grade stainless steel to ensure the sanitation and safety of the fermentation process.

[0008] Furthermore, the fermentation tank body is cylindrical with a flat bottom.

[0009] Furthermore, the external circulation ultraviolet sterilization device includes a delivery pump connected to the ultraviolet circulation pipeline.

[0010] Furthermore, a discharge port is provided at the bottom of the fermentation tank body, which is used to connect the inside and outside of the fermentation tank. The discharge port is provided with a three-way valve, and the three-way valve is provided with a third valve and a fourth valve; one direction of the three-way valve is connected to the discharge pipe, and the fourth valve is used to control the discharge of the feed liquid from the tank body to the outside of the tank body; the other direction is connected to the input end of the delivery pump of the external circulation ultraviolet sterilization device, and the third valve is used to control the feed liquid to flow from the fermentation tank body to the input end of the delivery pump.

[0011] Furthermore, the ultraviolet circulation pipeline has a lower pipe opening and an upper pipe opening; the lower pipe opening is connected to the output end of the delivery pump, and the feed liquid is pumped into the ultraviolet circulation pipeline by the delivery pump through the lower pipe opening; the upper pipe opening is connected to the side of the fermentation tank body, and the feed liquid in the ultraviolet circulation pipeline is controlled by the fifth valve to flow from the upper pipe opening into the fermentation tank body.

[0012] Furthermore, the lactic acid bacteria-specific fermentation device includes an ozone sterilization device; the ozone sterilization device includes an ozone generator, a microporous aeration pipe and an air supply pipe, the ozone generator is connected to the microporous aeration pipe through the air supply pipe, and the microporous aeration pipe is arranged at the inner bottom of the fermentation tank body.

[0013] Furthermore, the ozone sterilization device uses air as raw material, and produces ozone in the ozone generator using the ionization principle, with an ozone production efficiency of 24g / h. The produced ozone is added to the fermentation tank containing the raw materials through the ozone pressure pump through the air supply pipe, and the microporous aeration tube forms tiny bubbles of ozone, allowing the ozone molecules to fully dissolve in the culture solution, ensuring that the ozone concentration in the culture solution reaches 800mg / L / h, which lasts for 0.1-2h; the half-life of ozone molecules at room temperature is about 16min, and the ozone remaining in the culture solution after sterilization will not cause harm to the seed bacteria.

[0014] Furthermore, one end of the microporous aeration tube is connected to the fermentation tank body and aerates the fermentation tank body, and the other end is connected to the air supply pipe; aeration nozzles are evenly distributed on the outer surface of the microporous aeration tube where it is connected to the fermentation tank body.

[0015] Furthermore, the lactic acid bacteria-specific fermentation device includes a control device and an oxygen supply device.

[0016] Furthermore, the control device includes a temperature sensor, a pH sensor and a controller connected to the sensor; the controller is used to control the running speed of the delivery pump. The control device is used to monitor and adjust the temperature and pH value during the fermentation process to achieve precise control of the fermentation conditions to ensure that the lactic acid bacteria ferment in the most suitable environment. The oxygen supply device is used to provide an appropriate amount of air to the fermentation tank to promote the growth and metabolic process of the lactic acid bacteria.

[0017] Furthermore, a heater is provided at the bottom of the fermentation tank body, and an exhaust port is provided at the top of the fermentation tank body.

[0018] Beneficial effects:

[0019] The utility model discloses a special fermentation device for lactic acid bacteria, which aims to provide a fermentation device with reasonable structure, simple operation and high sterilization efficiency, so as to meet the requirements of the lactic acid bacteria fermentation process.

[0020] (1) Reasonable structure: The structural design of each component is reasonable, which is easy to install, maintain and clean, and improves the operability and stability of the device. Generally, bacterial fermentation requires stirring to achieve mass transfer of materials, oxygen, etc. to meet the needs. However, lactic acid bacteria grow better without stirring conditions and are more suitable for high-density fermentation of lactic acid bacteria. In view of this, the utility model removes the stirring device on the basis of the traditional stirring tank and uses an external circulation pump and an external circulation pipeline to achieve a mass transfer effect, which is more conducive to the fermentation of lactic acid bacteria.

[0021] (2) High sterilization efficiency: By combining the ozone device and ultraviolet sterilization, high-efficiency sterilization is achieved, and the sterilization rate of miscellaneous bacteria reaches more than 99%, which improves the fermentation success rate. The sterilization method of the utility model replaces the traditional high-temperature and high-pressure steam sterilization, and while achieving sterilization, the equipment is further used to achieve a uniform fermentation liquid effect, thereby reducing the fermentation cost and improving the safety of the fermentation process. It is generally applicable to lactic acid bacteria fermentation.

[0022] Therefore, the lactic acid bacteria special fermentation device has broad application prospects and economic benefits in the field of lactic acid bacteria fermentation production. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods, and the above and / or other advantages of the present invention will become more clear.

[0024] Figure 1This is a schematic diagram of a fermentation device for lactic acid bacteria. 1-tank body, 2-feeding port, 3-observation port, 4-control panel, 6-temperature electrode, 7-sampling port, 8-first valve, 9-exhaust port, 10-second valve, 11-pressure gauge, 12-discharge port, 13-third valve, 14-fourth valve, 15-bracket, 16-transfer pump, 17-ultraviolet lamp, 18-ultraviolet circulation pipeline, 19-fifth valve, 20-ozone generator, 21-ozone pressure pump, 22-sixth valve, 24-air supply pipe.

[0025] Figure 2 A schematic diagram of the three-dimensional structure of the lactic acid bacteria fermentation device. 5-heater, 23-microporous aeration pipe. DETAILED DESCRIPTION

[0026] The specific embodiment of the lactic acid bacteria-specific fermentation device described in this patent is described below.

[0027] The structural diagram of the lactic acid bacteria fermentation device is as follows: Figure 1 As shown, it includes the following components:

[0028] 1. Fermentation tank body 1: Made of food-grade stainless steel, with a capacity of 50L, supported by a bracket 15. The fermentation tank body 1 has a cylindrical shape and a flat bottom to ensure that the fermented material is fully mixed while meeting the requirements of static fermentation of lactic acid bacteria. The flat structure is more conducive to the lactic acid bacteria to be in uniform contact with the upper liquid after sinking to the bottom. The fermentation tank body 1 has no jacket on the tank wall and no stirring shaft in the tank. The fermentation temperature is controlled by the tank bottom heater 5. The heater 5 is a direct electric heater with a fast heating speed. Figure 2 As shown in the schematic diagram of the three-dimensional structure in the A direction. A feeding port 2, an observation port 3, a control panel 4 and an exhaust port 9 are provided on the top of the fermentation tank body 1. The exhaust port 9 is connected to one end of the exhaust pipe, and a second valve 10 and a pressure gauge 11 are also provided near the other end of the exhaust pipe. The sampling port 7 is arranged on the side of the fermentation tank body 1, and a first valve 8 is arranged near the sampling port 7 for controlling sampling. A discharge port 12 is provided at the bottom of the fermentation tank body 1. The discharge port 12 is provided with a three-way valve, one of which is connected to the discharge pipe, and the discharge is controlled by the fourth valve 14. The fermentation degree of the bacterial agent is observed through the observation port 3. After the sampling port takes a sample to determine the fermentation degree of the lactic acid bacteria, it is decided whether to open the fourth valve 14 for discharge. The other direction is connected to the external circulation ultraviolet sterilization device, and the third valve 13 is used to control whether the feed liquid is input into the external circulation ultraviolet sterilization device.

[0029] 2. External circulation ultraviolet sterilization device: The external circulation ultraviolet sterilization device is arranged on the outer side of the tank body 1. The feed liquid in the fermentation tank body 1 is transported to the ultraviolet circulation pipe 18 through the discharge port 12 by the delivery pump 16 at a flow rate of 10L / min. The ultraviolet circulation pipe 18 is provided with an ultraviolet lamp 17 on one side. The ultraviolet circulation pipeline 18 has a lower pipe mouth and an upper pipe mouth; the lower pipe mouth is connected to the output end of the delivery pump 16, and the input end of the delivery pump 16 is connected to the discharge port 12 located at the bottom of the fermentation tank body 1. The third valve 13 is used to control the liquid to flow from the fermentation tank body 1 to the input end of the delivery pump 16, and then the delivery pump 16 is pumped into the ultraviolet circulation pipeline 18 through the lower pipe mouth; the upper pipe mouth is connected to the side of the fermentation tank body 1, and the fifth valve 19 is used to control the liquid in the ultraviolet circulation pipeline 18 to flow from the upper pipe mouth into the fermentation tank body 1. The third valve 13 and the fifth valve 19 are used to control the disconnection of the external circulation ultraviolet sterilization device and the fermentation tank body 1. The volume of the liquid in the ultraviolet circulation pipe 18 is small, the pipe diameter is small, the ultraviolet lamp 17 irradiates the liquid at a short distance, the ultraviolet light irradiates the entire surface, and the liquid flows and sterilizes, so that the liquid in the fermentation tank can be irradiated by ultraviolet rays multiple times and fully, and the sterilization is more thorough; the ultraviolet lamp 17 is powered on by the electric control device located above the ultraviolet circulation pipe 18, and the wavelength of the ultraviolet lamp 17 is selected to be 254nm, and the power size varies according to the capacity of different fermentation tanks, and the sterilization time is 0.1-2h. As the ultraviolet light propagates over a longer distance, its energy weakens and the sterilization effect weakens. The fermentation device described in this article realizes ultraviolet sterilization within a short distance (ultraviolet circulation pipe 18) through external circulation, and the sterilization effect is improved. In addition, the ultraviolet circulation pipe 18 and the delivery pump 16 in the external circulation ultraviolet sterilization device can also operate during the lactic acid bacteria fermentation process. The delivery pump 16 delivers the fermentation liquid in the fermentation tank body 1 to the ultraviolet circulation pipe 18, and then returns to the tank body 1. The ultraviolet lamp 17 is turned off during this process. The external circulation setting can make the strains in the fermentation process mix evenly, avoid the use of a stirring shaft that causes a decrease in the survival rate of the strains, etc., which is more conducive to the high-density fermentation of lactic acid bacteria.

[0030] 3. Ozone sterilization device: It includes an ozone generator 20, an air supply pipe 24 and a microporous aeration pipe 23. The ozone generator 20 is connected to the microporous aeration pipe 23 through the air supply pipe 24. The microporous aeration pipe 23 is arranged at the bottom of the inner side of the fermentation tank body 1. The sixth valve 22 controls the disconnection of ozone and the fermentation tank body 1. Aeration nozzles are evenly distributed on the outer surface of the microporous aeration pipe 23 where it is connected to the fermentation tank body 1. The ozone sterilization device uses air as raw material and produces ozone in the ozone generator 20 using the ionization principle. The ozone production efficiency is 24g / h. The produced ozone is added to the fermentation tank body 1 filled with feed liquid by the ozone pressure pump 21 through the air supply pipe 24. The microporous aeration pipe 23 makes the ozone form tiny bubbles, allowing the ozone molecules to fully dissolve in the feed liquid, ensuring that the ozone concentration in the feed liquid is ≥800mg / L / h, which lasts for 20 minutes; the half-life of ozone molecules at room temperature is about 16 minutes. After the sterilization is completed, the residual ozone in the culture liquid will not cause harm to the seed bacteria. The microporous aeration pipe 23 is fixed to the bottom of the inner side of the fermentation tank body 1, such as Figure 2 The A-axis three-dimensional structure is shown in the schematic diagram.

[0031] 4. Control device: including temperature sensor, temperature electrode 6, pH sensor, pH electrode and controller. The temperature sensor and pH sensor are connected to the controller and are respectively installed in the fermentation tank body 1 to monitor the temperature and pH value during the fermentation process in real time. The controller adjusts the pump speed of the delivery pump according to the data fed back by the sensor to keep the temperature and pH value within the set range. The fermentation tank body 1 is provided with a temperature electrode 6 for detecting the temperature of the bacterial agent fermentation in the fermentation tank body 1. The tank body 1 is also provided with a temperature control device, which can realize constant temperature cultivation according to relevant process requirements. The temperature control device uses direct electric heating of the heater 5, which has a fast heating speed and cyclic heating, avoiding the problem of scorching of the culture medium due to local overheating, and avoiding the problem of special equipment such as double-layer fermentation tanks and steam generators required for interlayer steam heating in the prior art, optimizing the overall equipment structure, reducing investment costs and production costs, and making the production process safer. A pH electrode is provided inside the fermentation tank body 1 to dynamically monitor the pH of the fermentation process. This fermentation tank is used as a special fermentation tank for lactic acid bacteria. The pH value of the fermentation liquid during the fermentation process can intuitively reflect the success of the fermentation. Therefore, it can greatly reduce the losses caused by the long culture cycle due to the failure to detect the fermentation failure in time, and maximize the use of the fermentation liquid of the failed fermentation.

[0032] The lactic acid bacteria-specific fermentation device of the utility model also includes an oxygen supply device and the like, which is a common device for fermentation tanks.

[0033] The utility model has a simple overall structure, low manufacturing cost and high cost performance. In this embodiment, the external circulation ultraviolet sterilization and the ozone steam sterilization are operated simultaneously, and the dual sterilization of ultraviolet and ozone can achieve a sterilization rate of more than 99%.

[0034] Example 1

[0035] The operation process of the embodiment of the lactic acid bacteria special fermentation device is as follows:

[0036] 1. Dissolve the fermentation raw materials with hot water, sterilize with high-pressure steam, and then add them into the fermentation tank body 1 through the feeding port 2 on the top of the fermentation tank body 1.

[0037] 2. Connect 1.0×10 7 CFU / mL of Escherichia coli strains, start the control system, and begin monitoring the temperature and pH value during the fermentation process.

[0038] 3. Simultaneously start the external circulation ultraviolet sterilization device and the ozone sterilization device. The ozone sterilization device uses an ozone pressure pump 21 to pump the ozone prepared by the ozone generator 20 into the microporous aeration tube 23 located at the bottom of the fermentation tank body 1 through the air supply pipe 24. The microporous aeration tube 23 causes the ozone to form tiny bubbles, allowing the ozone molecules to fully dissolve in the culture solution in the fermentation tank body 1. The ozone generator 20 has an ozone production efficiency of 24g / h, and the ozone concentration in the culture solution in the tank body 1 reaches 800mg / L / h. At the same time, the third valve 13 located below the fermentation tank body 1 and the fifth valve 19 located on the side of the fermentation tank body 1 are opened, and the culture medium in the fermentation tank body 1 can be transported to the ultraviolet circulation pipe 18 at a flow rate of 10L / min by the delivery pump 16 through the discharge port 12. The culture medium is irradiated and sterilized by the 254nm ultraviolet lamp 17 in the ultraviolet circulation pipe, and then the culture medium is flowed into the fermentation tank body 1 from the upper pipe port of the ultraviolet circulation pipe 18 by the delivery pump 16 through the external circulation ultraviolet sterilization device. The external circulation ultraviolet sterilization device can realize the flow sterilization of the culture solution. The sterilization operation is performed for 20 minutes.

[0039] 4. After sterilization, open the first valve 8, take 1 mL of sample from the sampling port 7 and dilute 10 4 times, take 100 μL and spread it on LB solid culture medium, culture it at 37°C overnight, and record the number of single colonies on the plate as 0, which means the sterilization rate reaches 100.0%.

[0040] Example 2 Fermentation of Lactobacillus plantarum

[0041] 1. Activation of strains: Take Lactobacillus plantarum strains (glycerol tube) and streak inoculate on MRS solid plates and culture at 37°C for 48 hours;

[0042] 2. Seed culture: Pick a mature single colony and transfer it into 500mL, filled with 200mL MRS liquid culture medium, the culture conditions are stirring speed 100r / min, culture temperature 37℃, and culture time 48h;

[0043] 3. Fermentation preparation: Dissolve the fermentation raw materials with hot water and add them into the fermentation tank body 1 through the feeding port 2 on the top of the fermentation tank body 1; start the ozone sterilization device and the external circulation ultraviolet sterilization device in the manner described in Example 1, and perform the sterilization operation for 20 minutes.

[0044] 4. Fermentation culture: The seed culture liquid is connected to the fermentation tank body 1 through the feeding port 2 at an inoculation rate of 10% by volume, the control system is started, and the controller begins to monitor the temperature and pH value during the fermentation process; according to the data fed back by the sensor, the controller adjusts the pump speed of the delivery pump 16 to 10L / min to keep the temperature and pH value stable during the fermentation process; during the fermentation process, the temperature, pH value and the state of the fermentation product are regularly monitored as needed, and the control system parameters are adjusted to maintain the stability of the fermentation process; the pressure gauge 11 is tested throughout the fermentation process to prevent the pressure in the tank from being too high. After the fermentation is completed, the control system and the oxygen supply device are turned off, the second valve 10 is opened, the exhaust gas is discharged through the exhaust port 9, the fourth valve 14 is opened to discharge the waste liquid, the fermentation tank is cleaned, and the entire fermentation cycle is completed.

[0045] The liquid filling coefficient in the fermentation tank is 60% v / v (MRS medium), and the culture conditions are: ventilation by an oxygen supply device, the ventilation volume is 2L / min, the culture temperature is 37°C, and the culture is carried out for 48 hours. When the pH drops below 4.0, the plant lactobacillus fermentation liquid is obtained, and the number of viable bacteria in the fermentation liquid is 10 9 CFU / mL.

[0046] Through this embodiment, the lactic acid bacteria-specific fermentation device can stably and efficiently carry out the fermentation production of lactic acid bacteria, thereby improving production efficiency and product quality, and has high practical value.

[0047] Example 3 Fermentation of mixed bacteria

[0048] 1. Activation of strains: Take Saccharomyces cerevisiae and Lactobacillus plantarum strains (glycerol tubes) and streak them on PDA and MRS solid plates respectively, and culture them at 37°C for 48 hours;

[0049] 2. Seed culture: Pick mature single colonies of Saccharomyces cerevisiae and Lactobacillus plantarum and transfer them into 500 mL of liquid culture medium containing 200 mL of PDA and MRS. The culture conditions are stirring speed of 100 r / min, culture temperature of 37°C, and culture time of 48 h.

[0050] 3. Fermentation preparation: dissolve the fermentation raw materials with hot water and add them into the fermentation tank through the top cover of the fermentation tank; start the ozone sterilization device and the external circulation ultraviolet sterilization device to perform sterilization operations.

[0051] 4. Fermentation tank culture: Saccharomyces cerevisiae and Lactobacillus plantarum seed culture solution are respectively inoculated in a volume ratio of 2% and 8% through the feed port 2 on the top of the tank body 1 into the fermentation tank body 1, the control system is started, and the controller begins to monitor the temperature and pH value during the fermentation process; according to the data fed back by the sensor, the controller adjusts the pump speed of the delivery pump 16 to 10L / min to keep the temperature and pH value stable during the fermentation process; during the fermentation process, the temperature, pH value and the state of the fermentation product are regularly monitored as needed, and the control system parameters are adjusted to maintain the stability of the fermentation process; the pressure gauge 11 is tested throughout the fermentation process to prevent the pressure in the tank from being too high. After the fermentation is completed, the control system and the oxygen supply device are turned off, the second valve 10 is opened, the exhaust gas is discharged through the exhaust port 9, the fourth valve 14 is opened to discharge the waste liquid, the fermentation tank is cleaned, and the entire fermentation cycle is completed.

[0052] The liquid filling coefficient in the fermentation tank is 60% by volume (molasses medium: molasses 20g / L, sodium acetate 5g / L, peptone 5g / L, yeast extract 5g / L, NaCl 5g / L, natural pH), and the culture conditions are: ventilation by an oxygen supply device, the ventilation volume is 2L / min, the culture temperature is 37°C, and the culture is carried out for 48h. When the pH drops below 4.0, a mixed bacterial fermentation liquid is obtained, and the viable counts of saccharomyces cerevisiae and Lactobacillus plantarum in the fermentation liquid are detected by sampling port 7, respectively. 8 CFU / mL, 10 9 CFU / mL.

[0053] Comparative Example 1 UV sterilization only

[0054] 1. Dissolve the fermentation raw materials with hot water, sterilize with high-pressure steam, and then add them into the fermentation tank body 1 through the feeding port 2 on the top of the fermentation tank body 1.

[0055] 2. Connect 1.0×10 7 CFU / mL of Escherichia coli strains, start the control system, and the controller begins to monitor the temperature and pH value during the fermentation process.

[0056] 3. According to the method described in Example 1, only the ultraviolet sterilization device is started and the sterilization operation is performed for 20 minutes.

[0057] 4. After sterilization, open the first valve 8, take 1 mL of sample from the sampling port 7 and dilute 10 4 times, take 100 μL and spread it on LB solid culture medium, culture it at 37°C overnight, and record the number of single colonies on the plate. If 15 colonies are found, the sterilization rate is 85.0%.

[0058] Comparative Example 2 Ozone sterilization only

[0059] 1. Dissolve the fermentation raw materials with hot water, sterilize with high-pressure steam, and then add them into the fermentation tank body 1 through the feeding port 2 on the top of the fermentation tank body 1.

[0060] 2. Connect 1.0×10 7 CFU / mL of Escherichia coli strains, start the control system, and the controller begins to monitor the temperature and pH value during the fermentation process.

[0061] 3. Start only the ozone sterilization device in the manner described in Example 1 and perform the sterilization operation for 20 minutes.

[0062] 4. After sterilization, take 1 mL of sample from the sampling port and dilute it 10 4 times, take 100 μL and spread it on LB solid culture medium, culture it at 37℃ overnight, and record the number of single colonies on the plate. If there are 10, the sterilization rate is 90.0%.

[0063] The above are only preferred specific implementation methods of the utility model, but the protection scope of the utility model is not limited to this. Any technician familiar with the technical field within the technical scope disclosed by the utility model, who makes equivalent replacements or changes based on the technical scheme and utility model concept of the utility model, should be covered by the protection scope of the utility model.

Claims

1. A lactic acid bacteria fermentation device, characterized in that: include: A fermentation tank body (1) and an external circulation ultraviolet sterilization device, wherein the external circulation ultraviolet sterilization device is located on the outer side of the fermentation tank body (1); the external circulation ultraviolet sterilization device comprises an ultraviolet circulation pipeline (18) connected to the fermentation tank body (1) and an ultraviolet lamp (17) located on one side of the ultraviolet circulation pipeline (18); the lactic acid bacteria-specific fermentation device also comprises an ozone sterilization device; the ozone sterilization device comprises an ozone generator (20), a microporous aeration pipe (23) and an air supply pipe (24); the ozone generator (20) is connected to the microporous aeration pipe (23) via the air supply pipe (24), and the microporous aeration pipe (23) is arranged at the bottom of the fermentation tank body (1).

2. The lactic acid bacteria-specific fermentation device according to claim 1, characterized in that: The external circulation ultraviolet sterilization device comprises a delivery pump (16) connected to the ultraviolet circulation pipeline (18).

3. The lactic acid bacteria-specific fermentation device according to claim 2, characterized in that: The bottom of the fermentation tank body (1) is provided with a discharge port (12), the discharge port (12) is provided with a three-way valve, and the three-way valve is provided with a third valve (13) and a fourth valve (14); one direction of the three-way valve is connected to the discharge pipe, and the fourth valve (14) is used to control the flow of liquid from the tank body (1) to the outside of the tank body (1); the other direction of the three-way valve is connected to the input end of a delivery pump (16) of an external circulation ultraviolet sterilization device, and the third valve (13) is used to control the flow of liquid from the fermentation tank body (1) to the input end of the delivery pump (16).

4. The lactic acid bacteria-specific fermentation device according to claim 3, characterized in that: The ultraviolet circulation pipeline (18) has a lower pipe opening and an upper pipe opening; the lower pipe opening is connected to the output end of the delivery pump (16), and the feed liquid is pumped into the ultraviolet circulation pipeline (18) through the lower pipe opening by the delivery pump (16); the upper pipe opening is connected to the side of the fermentation tank body (1), and the feed liquid in the ultraviolet circulation pipeline (18) is controlled by a fifth valve (19) to flow from the upper pipe opening into the fermentation tank body (1).

5. The lactic acid bacteria-specific fermentation device according to claim 1, characterized in that: One end of the microporous aeration pipe (23) is connected to the fermentation tank body (1) and aerates the fermentation tank body (1), and the other end is connected to the air supply pipe (24); aeration nozzles are evenly distributed on the outer surface of the microporous aeration pipe (23) at the position where it is connected to the fermentation tank body (1).

6. The lactic acid bacteria-specific fermentation device according to claim 1, characterized in that: The lactic acid bacteria-specific fermentation device comprises a control device and an oxygen supply device.

7. The lactic acid bacteria-specific fermentation device according to claim 6, characterized in that: The control device comprises a temperature sensor, a pH sensor and a controller connected to the sensors; the controller is used to control the running speed of the delivery pump (16).

8. The lactic acid bacteria-specific fermentation device according to claim 1, characterized in that: A heater (5) is arranged at the bottom of the fermentation tank body (1).

9. The lactic acid bacteria-specific fermentation device according to claim 1, characterized in that: The top of the fermentation tank body (1) is provided with an exhaust port (9).

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