Cell identifying, selecting and transplanting device

Abstract

The utility model relates to a cell identifying, selecting and transplanting device which comprises a marble bottom plate, an XYZ three-axis cell extraction module, a separating and screening module, a visual detection module and a blanking module, the XYZ three-axis cell extraction module comprises an XYZ three-axis moving module and an extraction needle module; the separating and screening module comprises an inverted microscope and an objective table; the extraction needle module is arranged above the objective table; the inverted microscope is arranged below the objective table; the visual detection module is used for guiding and positioning the XYZ three-axis cell extraction module and realizing displacement compensation; after the separating and screening module completes screening through vision, the XYZ three-axis cell extraction module sucks the cells to the discharging module; the discharging module is provided with a culture dish secondary positioning mechanism. Compared with manual cell selection, the efficiency of manual cell extraction can be improved; operations such as boring cell selection and easy errors are omitted, and the yield of manual cell selection is improved; and the device has strong universality, universality and use value in different fields and different products.

Description

Technical Field

[0001] The utility model relates to the technical field of visual detection, in particular to a cell identification, selection and transplantation device. Background Art

[0002] Most traditional cell picking methods involve observing cells under a microscope and then manually aspirating the cells with a handheld aspiration device. However, manual picking methods usually have the following disadvantages:

[0003] 1. Operation accuracy and stability issues: Since the operation process needs to be performed manually, it involves the skills and experience of the operator. There may be differences in operation accuracy and stability between different personnel, which may lead to inaccurate or unstable cell aspiration. In addition, long-term manual operation may cause fatigue, further affecting the accuracy of the operation.

[0004] 2. Efficiency issue: Manual handheld aspiration of cells is usually a relatively slow process, especially when the amount or position of aspiration needs to be precisely controlled. This inefficiency may not be suitable for experiments or application scenarios that require rapid processing of a large number of samples.

[0005] 3. Risk of cross contamination: In the process of aspirating cells, if the operation is improper or the equipment is not cleaned thoroughly, cross contamination between samples may occur. This contamination may not only affect the accuracy of the experimental results, but also cause irreversible effects on the research.

[0006] 4. Potential damage to cells: Due to the limitation of manual operation, it may not be possible to accurately control the suction force and speed, which may cause damage or destruction to cells. For experiments with high requirements on cell status, this damage may be fatal.

[0007] 5. Poor repeatability and consistency: Due to the variability and uncertainty of manual operation, the results of cell aspiration using manual handheld aspiration devices may perform poorly in terms of repeatability and consistency. This is an important issue for studies that require accurate comparison of different samples or experimental conditions. Utility Model Content

[0008] The technical problem to be solved by the utility model is to provide a cell identification, selection and transplantation device, which has the characteristics of good continuity, stability and high precision.

[0009] The utility model adopts the following technical scheme to solve the technical problems: a cell identification, selection and transplantation device, comprising a marble bottom plate, on which an XYZ three-axis cell extraction module, a separation and screening module, a visual inspection module and a material discharge module are arranged; the XYZ three-axis cell extraction module comprises an XYZ three-axis moving module and an extraction needle module; the separation and screening module comprises an inverted microscope and a stage; the extraction needle module is arranged above the stage; the inverted microscope is arranged below the stage; the visual inspection module guides and positions the XYZ three-axis cell extraction module and realizes displacement compensation; after the separation and screening module completes the screening by vision, the XYZ three-axis cell extraction module absorbs the cells to the material discharge module; the material discharge module is provided with a secondary positioning mechanism for a culture dish.

[0010] Furthermore, the XYZ three-axis moving module described in the utility model includes an X-axis moving module, a Y-axis moving module is arranged on the X-axis moving module, and a Z-axis moving module is arranged on the Y-axis moving module; the extraction needle module is arranged on the Z-axis moving module.

[0011] Furthermore, the extraction needle module described in the utility model includes an extraction needle, and the extraction needle is connected to a plunger pump and a peristaltic pump respectively through a Luer connector and a pump adapter.

[0012] Furthermore, the loading platform of the present invention comprises an XY electric module, a culture dish is placed on the loading platform, and the culture dish is moved by the XY electric module.

[0013] Furthermore, the visual inspection module described in the utility model includes a frame, a Z-axis imaging inspection module is arranged on the top of the frame, and an XY-axis imaging inspection module is arranged on one side of the frame.

[0014] Furthermore, the culture dish secondary positioning mechanism of the utility model comprises a base plate and a positioning block and a limiting block arranged on the base plate; the limiting block is fixed on the base plate, and the positioning block is movably arranged on the base plate through an elastic member.

[0015] The beneficial effect of the utility model is that it solves the defects existing in the background technology. Compared with manual operation of selecting cells, the utility model can screen and mark all cells in the well plate at one time, confirm the cells to be selected by an inverted microscope, and extract the cells into the culture dish in batches, thereby improving the efficiency of manual cell extraction; compared with manual operation of selecting cells, some areas are repeatedly selected, and cells in some areas are missed, the utility model can save the boring and error-prone operations of selecting cells, and improve the yield rate of manual cell selection; the utility model has strong versatility, extensiveness and use value in different fields and different products. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the utility model;

[0017] Figure 2 It is a structural schematic diagram of the rotary suction module of the utility model;

[0018] Figure 3 It is a structural schematic diagram of the separation and screening module of the utility model;

[0019] Figure 4 It is a structural schematic diagram of the visual inspection module and the material handling module of the utility model;

[0020] In the figure: 1. XYZ three-axis cell extraction module; 2. Separation and screening module; 3. Visual inspection module; 4. Unloading module; 5. Marble bottom plate;

[0021] 11. XYZ three-axis moving module; 12. Extraction needle module; 13. X-axis moving module; 14. Y-axis moving module; 15. Z-axis moving module; 16. Extraction needle; 17. Luer connector;

[0022] 21. Stage; 22. Inverted microscope module; 23. XY motorized module; 24. Culture dish;

[0023] 31. Frame; 32. Z-axis imaging detection module; 33. XY-axis imaging detection module;

[0024] 41. Secondary positioning mechanism; 42. Base plate; 43. Positioning block; 44. Limiting block. DETAILED DESCRIPTION

[0025] Now the utility model is further described in detail in conjunction with the accompanying drawings and preferred embodiments. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the utility model in a schematic manner, and therefore only show the components related to the utility model.

[0026] like Figure 1 The cell identification, selection and transplantation device shown is applicable to cells or bacteria and other similar microbial monomers; it includes an XYZ three-axis cell extraction module 1, a separation and screening module 2, a visual inspection module 3 and a down-transport module 4 arranged on a marble bottom plate 5; the device has very high precision, and the overall repeatability can reach ±0.005MM, which can more accurately absorb cells. The bottom plate of the device is made of marble, which has high processing precision, small deformation, and can prevent the machine from shaking, thereby ensuring the detection accuracy.

[0027] like Figure 2As shown, the XYZ three-axis cell extraction module 1 includes an XYZ three-axis moving module 11 and an extraction needle module 12; the XYZ three-axis moving module 11 includes an X-axis moving module 13, on which a Y-axis moving module 14 is arranged, and on which a Z-axis moving module 15 is arranged; the extraction needle module is arranged on the Z-axis moving module.

[0028] The extraction needle module 12 includes an extraction needle 16, which is connected to a plunger pump and a peristaltic pump respectively through a Luer connector 17 and a pump adapter. The liquid suction device used uses a combination of an inexpensive plunger pump and a peristaltic pump to reduce costs while ensuring micro-aspiration in a stable manner. The liquid suction device controls the cell extraction needle in a micro-amount through the plunger pump. In order to remove air from the cell needle and the pipeline, another peristaltic pump is used to fill the liquid into the plunger pump and the pipeline. After the pipeline is filled with liquid, the valve is closed to isolate the peristaltic pump from the plunger pump liquid to prevent the liquid in the plunger pump from flowing back.

[0029] like Figure 3 As shown, the separation and screening module 2 includes a stage 21 and an inverted microscope module 22 disposed below the stage; the stage includes an XY electric module 23, a culture dish 24 is placed on the stage, and the culture dish is moved by the XY electric module. A constant temperature device is also disposed on the stage.

[0030] The inverted microscope used has an XY stage, which can center the cells to be absorbed directly above the camera. There is a switch between bright field and fluorescence mode. In the bright field state, the light source is turned on; in the fluorescence state, the light source is turned off. It can be applied to a variety of scenarios. It can switch between 4x, 10x, 20x, and 40x microscopes to observe cells under multiple microscopes. The rotatable light source can avoid absorbing cell structures and save equipment space. The constant temperature table that can maintain the temperature of the cells keeps the temperature at room temperature and the cells remain active.

[0031] The visual inspection module 3 guides the rotation absorption module to locate and realize the displacement compensation; Figure 4 As shown, the visual inspection module includes a frame 31, a Z-axis imaging inspection module 32 is arranged on the top of the frame, and an XY-axis imaging inspection module 33 is arranged on one side of the frame.

[0032] After the cell extraction needle is manually installed, the visual guidance station is required to guide the needle height and position and realize displacement compensation. The XY axis imaging detection module detects the X and Y axis coordinates of the needle; the Z axis imaging detection module detects the Z axis height of the needle.

[0033] The unloading module 4 includes a secondary positioning mechanism 41 for a culture dish, which includes a base plate 42, a positioning block 43 and a limit block 44 arranged on the base plate; the limit block is fixed on the base plate, and the positioning block is movably arranged on the base plate through an elastic member. During the secondary positioning, the culture dish is placed in the gap between the positioning block and the limit block, and then the positioning block is pushed by the elastic member to make the culture dish close to the limit block.

[0034] The utility model takes pictures through an inverted microscope camera, and the electric stage moves through the XY axis, uses vision to classify and mark cells, and obtains the cell XYZ coordinates through an algorithm. The three-axis linear motor module moves to the position according to the known coordinates, and then uses the plunger pump to micro-absorb cells into the needle tip, and puts the target cells into the culture dish after the absorption is completed. The specific action process is as follows: Step 1: Manually place the culture dish A to be screened on the stage; Step 2: Manually place the culture dish B to secondary positioning; Step 3: Manually install the cell extraction needle; Step 4: The electric stage moves through the XY axis, and uses vision to select the required cells; Step 5: The cell extraction needle moves to the visual detection calibration coordinates through the XYZ three-axis manipulator; Step 6: The cell extraction needle passes through the XYZ three-axis manipulator to the culture dish A to absorb the selected cells; Step 7: After absorbing the cells, put the cells into the culture dish B; Step 8: After completing the cell selection of all target cells, prompt to manually take away the culture dish B.

[0035] The above description only describes the specific implementation methods of the utility model. Various examples do not limit the essential content of the utility model. Ordinary technicians in the relevant technical field can modify or deform the specific implementation methods described above after reading the description without departing from the essence and scope of the utility model.

Claims

1. A cell identification, selection and transplantation device, characterized in that: It comprises a marble bottom plate, on which an XYZ three-axis cell extraction module, a separation and screening module, a visual inspection module and a material discharge module are arranged; the XYZ three-axis cell extraction module comprises an XYZ three-axis moving module and an extraction needle module; the separation and screening module comprises an inverted microscope and a stage; the extraction needle module is arranged above the stage; the inverted microscope is arranged below the stage; the visual inspection module guides and positions the XYZ three-axis cell extraction module and realizes displacement compensation; after the separation and screening module completes the screening by vision, the XYZ three-axis cell extraction module absorbs the cells to the material discharge module; the material discharge module is provided with a secondary positioning mechanism for the culture dish.

2. A cell identification, selection and transplantation device as claimed in claim 1, characterized in that: The XYZ three-axis moving module comprises an X-axis moving module, a Y-axis moving module is arranged on the X-axis moving module, a Z-axis moving module is arranged on the Y-axis moving module; and the extraction needle module is arranged on the Z-axis moving module.

3. The cell identification, selection and transplantation device according to claim 1, characterized in that: The extraction needle module comprises an extraction needle, and the extraction needle is connected to a plunger pump and a peristaltic pump respectively through a Luer connector and a pump adapter.

4. The cell identification, selection and transplantation device according to claim 1, characterized in that: The stage comprises an XY electric module, a culture dish is placed on the stage, and the culture dish is moved by the XY electric module.

5. The cell identification, selection and transplantation device according to claim 1, characterized in that: The visual inspection module comprises a frame, a Z-axis imaging inspection module is arranged on the top of the frame, and an XY-axis imaging inspection module is arranged on one side of the frame.

6. The cell identification, selection and transplantation device according to claim 1, characterized in that: The culture dish secondary positioning mechanism comprises a base plate and a positioning block and a limiting block arranged on the base plate; the limiting block is fixed on the base plate, and the positioning block is movably arranged on the base plate through an elastic member.

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