Cell culture device

Abstract

The utility model discloses a cell culture device. The cell culture device comprises a cabinet body and an adaptive clamping mechanism, a rotating column is rotatably connected to the center of the bottom wall of the cabinet body, uniformly distributed turntables are fixedly connected to the upper end of the rotating column, uniformly distributed placement grooves are formed in the turntables, culture dishes are arranged in the placement grooves, and ventilation openings are formed in the left end and the right end of the cabinet body; the adaptive clamping mechanisms are arranged in the placement grooves, the adaptive clamping mechanisms and vertically adjacent culture dishes are installed in a matched mode, a PLC is arranged at the right end of the front side surface of the cabinet body, the input end of the PLC is electrically connected with an external power source, and each adaptive clamping mechanism comprises a fixing groove, a telescopic column, a spring, a clamping plate and an elastic clamp. According to the cell culture device, culture dishes of different specifications can be rapidly and stably clamped, meanwhile, the liquid injection requirements of the culture dishes of different specifications can be met, and the culture efficiency of the cell culture device is effectively improved.

Description

Technical Field

[0001] The utility model relates to the technical field of laboratory equipment, in particular to a cell culture device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture is also called cell cloning technology. The formal term in biology is cell culture technology. Cell culture itself is the large-scale cloning of cells. Cell culture technology can transform a cell into a simple single cell or a multi-cell with very little differentiation through large-scale culture. This is an indispensable part of cloning technology. Cell culture itself is the cloning of cells. Cell culture devices are the main equipment for cell culture. The performance of cell culture devices has an important influence on the results of cell culture.

[0003] Some existing cell culture devices use a clamping unit with fixed specifications to clamp and fix culture dishes of specific specifications, and some cell culture devices use artificial injection to supplement the nutrient solution inside the culture dish.

[0004] There are some problems now. For example, the use of a clamping unit with fixed specifications may limit the range of culture dishes applicable to the cell culture device, and the use of artificial injection may affect the efficiency of cell culture in the cell culture device. Utility Model Content

[0005] The technical problem to be solved by the utility model is to overcome the existing defects and provide a cell culture device that can realize fast and stable clamping of culture dishes of different specifications, and at the same time can meet the injection requirements of culture dishes of different specifications, effectively improve the culture efficiency of the cell culture device, and can effectively solve the problems in the background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture device, comprising a cabinet and an adaptive clamping mechanism;

[0007] Cabinet: The center of the bottom wall is rotatably connected with a rotating column, the upper end of the rotating column is fixedly connected with evenly distributed turntables, the inside of the turntables are provided with evenly distributed placement slots, the inside of the placement slots are provided with culture dishes, and the left and right ends of the cabinet are provided with ventilation holes;

[0008] Adaptive clamping mechanisms: They are all arranged inside the placement groove. The adaptive clamping mechanisms are installed in conjunction with the vertically adjacent culture dishes, which can achieve fast and stable clamping of culture dishes of different specifications, and at the same time can meet the injection requirements of culture dishes of different specifications, effectively improving the culture efficiency of the cell culture device.

[0009] Furthermore, a PLC controller is provided at the right end of the front surface of the cabinet, and an input end of the PLC controller is electrically connected to an external power supply to control various electrical appliances.

[0010] Furthermore, the adaptive clamping mechanism includes a fixed groove, a telescopic column, a spring, a splint and an elastic clip. The fixed grooves are arranged in the middle of the placement grooves, circular grooves are arranged at the front and rear ends of the fixed grooves, telescopic columns are arranged inside the circular grooves, and the telescopic ends of the telescopic columns are fixedly connected with splints. Two longitudinally adjacent splints are installed in cooperation with the lower end of the same culture dish, and springs are fixedly connected between the splints and the longitudinally adjacent circular grooves. The springs are movably mounted on the outer surfaces of the vertically adjacent telescopic columns. The upper ends of the placement grooves are provided with symmetrically distributed elastic clips, and two laterally adjacent elastic clips are installed in cooperation with the middle of the same culture dish to achieve rapid and stable clamping of the culture dish.

[0011] Furthermore, an electric push rod is provided at the front end of the bottom wall of the cabinet, the telescopic end of the electric push rod is fixedly connected to an adjustment frame, the upper end of the adjustment frame is provided with a guide tube, the middle part of the guide tube is provided with evenly distributed nozzles, the nozzles are installed in cooperation with vertically adjacent culture dishes, the input end of the electric push rod is electrically connected to the output end of the PLC controller to realize the position adjustment of the guide tube.

[0012] Furthermore, a liquid storage tank is provided at the upper end of the cabinet, and an infusion pump is provided on the bottom wall of the liquid storage tank. The liquid outlet of the infusion pump is connected with the upper end of the guide tube through a hose, and a feed pipe is provided at the upper end of the liquid storage tank. The input end of the infusion pump is electrically connected to the output end of the PLC controller to provide driving force for the injection work.

[0013] Furthermore, the side walls of the cabinet are provided with symmetrically distributed heaters, the front end of the top wall of the cabinet is provided with a temperature and humidity sensor, the inside of the right vent is provided with an induced draft fan, the lower end of the cabinet is provided with a motor, the output shaft of the motor is fixedly connected to the lower end of the rotating column, the input ends of the motor, the heater and the induced draft fan are all electrically connected to the output end of the PLC controller, and the temperature and humidity sensor is electrically connected to the PLC controller in a bidirectional manner, so as to realize the control of the temperature and humidity inside the cabinet and ensure that the culture medium inside the culture dish is heated evenly.

[0014] Furthermore, the front end of the cabinet is hinged with a cabinet door through a hinge, the middle of the cabinet door is provided with an observation window, the lower end of the cabinet is fixedly connected with evenly distributed legs, and the lower ends of the legs are provided with evenly distributed mounting holes, which are convenient for the storage and retrieval of culture dishes and the observation of the cell culture status, while effectively ensuring the smooth progress of cell culture work.

[0015] Compared with the prior art, the beneficial effects of the present invention are: the cell culture device has the following advantages:

[0016] 1. Personnel place culture dishes containing cells to be cultured into the placement grooves one by one, and the lower end of the culture dish pushes the clamp to move in the direction away from the center of the vertically adjacent placement grooves. The clamps move toward each other so that the telescopic ends of the longitudinally adjacent telescopic columns contract, and at the same time, the longitudinally adjacent springs are elastically compressed. Under the elastic force of the springs, the longitudinally adjacent clamps can stably clamp the lower ends of the vertically adjacent culture dishes. At the same time, the middle part of the culture dish pushes the elastic clamp to move in the direction away from the center of the vertically adjacent placement grooves. Under the elastic action of the elastic clamp, the middle part of the vertically adjacent culture dish is stably clamped, which can realize fast and stable clamping of culture dishes of different specifications, and effectively improve the culture efficiency of the cell culture device.

[0017] 2. When liquid injection is required, the personnel controls the motor operation through the PLC controller. When the three culture dishes adjacent to each other in the longitudinal direction rotate to the liquid injection area, the PLC controller turns off the motor and realizes the operation of the electric push rod. The telescopic end of the electric push rod contracts, driving the adjustment frame to move downward, thereby causing the guide tube to move downward. The downward movement of the guide tube drives the evenly distributed nozzles to move downward. When the nozzle reaches the required height, the electric push rod is turned off by the PLC controller, and the infusion pump is operated. The infusion pump pumps the nutrient solution inside the storage tank into the inside of the guide tube through the hose, and then injects it into the corresponding culture dish through the nozzle. When the culture dish is filled, the PLC controller turns off the infusion pump and realizes the operation of the motor, moves the culture dish that has been filled out of the liquid injection area, and moves the culture dish to be filled to the liquid injection area. Then the PLC controller realizes the operation of the infusion pump to fill the nutrient solution, circulates in sequence, and realizes the nutrient solution filling work of all culture dishes. At the same time, it can meet the liquid injection needs of culture dishes of different specifications, effectively improving the culture efficiency of the cell culture device. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the utility model;

[0019] Figure 2 It is a schematic diagram of the cross-sectional structure inside the utility model;

[0020] Figure 3 It is a schematic diagram of the enlarged structure of point A of the utility model.

[0021] In the figure: 1 cabinet, 2 rotating column, 3 turntable, 4 adaptive clamping mechanism, 41 fixed slot, 42 telescopic column, 43 spring, 44 clamp, 45 elastic clip, 5 placement slot, 6 culture dish, 7 motor, 8 guide tube, 9 adjustment frame, 10 electric push rod, 11 infusion pump, 12 liquid storage tank, 13 temperature and humidity sensor, 14 heater, 15 vent, 16 induced draft fan, 17 cabinet door, 18 PLC controller, 19 support leg. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figure 1-3 ,This embodiment provides a technical solution: a cell culture device, including a cabinet 1 and an adaptive clamping mechanism 4;

[0024] Cabinet 1: A rotating column 2 is rotatably connected to the center of its bottom wall, and a uniformly distributed turntable 3 is fixedly connected to the upper end of the rotating column 2. The inside of the turntable 3 is provided with uniformly distributed placement slots 5, and the inside of the placement slots 5 is provided with culture dishes 6. Ventilation holes 15 are provided at both left and right ends of the cabinet 1. A PLC controller 18 is provided at the right end of the front surface of the cabinet 1. The input end of the PLC controller 18 is electrically connected to an external power supply. A cabinet door 17 is hinged at the front end of the cabinet 1 through a hinge, and an observation window is provided in the middle of the cabinet door 17. The lower end of the cabinet 1 is fixedly connected to uniformly distributed legs 19, and the lower ends of the legs 19 are provided with uniformly distributed mounting holes;

[0025] Adaptive clamping mechanism 4: They are all arranged inside the placement groove 5, and the adaptive clamping mechanism 4 is installed in cooperation with the vertically adjacent culture dish 6. The adaptive clamping mechanism 4 includes a fixed groove 41, a telescopic column 42, a spring 43, a clamping plate 44 and an elastic clamp 45. The fixed groove 41 is arranged in the middle of the placement groove 5, and circular grooves are arranged at the front and rear ends of the fixed groove 41. Telescopic columns 42 are arranged inside the circular grooves, and the telescopic ends of the telescopic columns 42 are fixedly connected with clamping plates 44. Two longitudinally adjacent clamping plates 44 are installed in cooperation with the lower end of the same culture dish 6, and springs 43 are fixedly connected between the clamping plates 44 and the longitudinally adjacent circular grooves. The springs 43 are movably sleeved on the outer surfaces of the vertically adjacent telescopic columns 42, and the upper ends of the placement grooves 5 are provided with symmetrically distributed elastic clamps. 45, two elastic clips 45 adjacent in the horizontal direction are installed in cooperation with the middle part of the same culture dish 6, and the culture dishes 6 containing the cells to be cultured are placed in the placement groove 5 in sequence, and the lower end of the culture dish 6 pushes the clamping plate 44 to move in the direction away from the center of the vertically adjacent placement groove 5, and the clamping plates 44 move towards each other so that the telescopic ends of the longitudinally adjacent telescopic columns 42 are contracted, and at the same time, the longitudinally adjacent springs 43 are elastically compressed. Under the elastic force of the springs 43, the longitudinally adjacent clamping plates 44 are stably clamped for the lower ends of the vertically adjacent culture dishes 6, and at the same time, the middle part of the culture dish 6 pushes the elastic clips 45 to move in the direction away from the center of the vertically adjacent placement groove 5, and under the elastic action of the elastic clips 45, the middle part of the vertically adjacent culture dishes 6 is stably clamped;

[0026] Among them: the front end of the bottom wall of the cabinet 1 is provided with an electric push rod 10, the telescopic end of the electric push rod 10 is fixedly connected with an adjustment frame 9, the upper end of the adjustment frame 9 is provided with a guide tube 8, the middle part of the guide tube 8 is provided with uniformly distributed nozzles, and the nozzles are installed in coordination with the vertically adjacent culture dishes 6, the input end of the electric push rod 10 is electrically connected to the output end of the PLC controller 18, the upper end of the cabinet 1 is provided with a liquid storage tank 12, the bottom wall of the liquid storage tank 12 is provided with an infusion pump 11, and the liquid outlet of the infusion pump 11 The cabinet 1 is connected to the upper end of the guide pipe 8 through a hose, a feed pipe is provided at the upper end of the liquid storage tank 12, and the input end of the infusion pump 11 is electrically connected to the output end of the PLC controller 18; the side wall of the cabinet 1 is provided with symmetrically distributed heaters 14, the front end of the top wall of the cabinet 1 is provided with a temperature and humidity sensor 13, the inside of the right ventilation port 15 is provided with an induced draft fan 16, the lower end of the cabinet 1 is provided with a motor 7, the output shaft of the motor 7 is fixedly connected to the lower end of the rotating column 2, and the motor 7, the heater 14 and the induced draft fan 16 are connected to the cabinet 1. The input ends of the fan 16 are electrically connected to the output ends of the PLC controller 18, the temperature and humidity sensor 13 is bidirectionally electrically connected to the PLC controller 18, the electric push rod 10 is running, the telescopic end of the electric push rod 10 is contracted, driving the adjustment frame 9 to move downward, and then the guide tube 8 is moved downward, and the guide tube 8 moves downward to drive the uniformly distributed nozzles to move downward. When the nozzle reaches the required height, the electric push rod 10 is closed by the PLC controller 18, and the infusion pump 11 is operated. The infusion pump 11 pumps the nutrient solution inside the liquid storage tank 12 into the inside of the guide tube 8 through the hose, and then injects it into the corresponding culture dish 6 through the nozzle. When the culture dish 6 is filled, the PLC controller 18 turns off the infusion pump 11 and realizes the operation of the motor 7, moves the culture dish 6 that has been filled out of the injection area, and moves the culture dish 6 to be filled to the injection area, and then the PLC controller 18 realizes the operation of the infusion pump 11 to fill the nutrient solution, and circulates in sequence to realize the nutrient solution filling work of all culture dishes 6.

[0027] The working principle of a cell culture device provided by the utility model is as follows: when working, firstly, the mounting holes evenly distributed at the lower ends of the legs 19 are stably connected with the threaded holes in the horizontal working area by bolts, thereby realizing the stable installation of the cabinet 1, the liquid storage tank 12 and other mechanisms. After the installation is stable, the personnel will place the culture dishes 6 containing the cells to be cultured into the placement grooves 5 in sequence, and the lower ends of the culture dishes 6 push the clamping plates 44 to move away from the center of the vertically adjacent placement grooves 5. The clamping plates 44 move towards each other, causing the telescopic ends of the longitudinally adjacent telescopic columns 42 to contract, and at the same time, the longitudinally adjacent springs 43 are elastically compressed. Under the elastic force of the springs 43, the longitudinally adjacent The clamping plate 44 stably clamps the lower ends of the vertically adjacent culture dishes 6, and the middle parts of the culture dishes 6 push the elastic clamps 45 to move away from the center of the vertically adjacent placement slots 5. Under the elastic action of the elastic clamps 45, the middle parts of the vertically adjacent culture dishes 6 are stably clamped. After the culture dishes 6 are all placed stably, the personnel close the cabinet door 17, and operate the temperature and humidity sensor 13 through the PLC controller 18 to monitor the temperature and humidity inside the cabinet 1 in real time, and transmit the monitoring information to the PLC controller 18. The PLC controller 18 adjusts the temperature and humidity inside the cabinet 1 by controlling the operation of the heater 14 and the induced draft fan 16. At the same time, the PLC controller The controller 18 realizes the operation of the motor 7. The output shaft of the motor 7 rotates to drive the rotating column 2 to rotate, and then drives the evenly distributed turntable 3 to rotate, so that the culture dishes 6 all rotate around the central axis of the rotating column 2, so that the culture medium inside the culture dishes 6 is evenly heated, which is convenient for cell culture. When liquid injection is required, the PLC controller 18 controls the motor 7 to operate. When the three culture dishes 6 adjacent to each other in the longitudinal direction rotate to the liquid injection area, the PLC controller 18 turns off the motor 7 and realizes the operation of the electric push rod 10. The telescopic end of the electric push rod 10 contracts, driving the adjustment frame 9 to move downward, thereby causing the guide tube 8 to move downward. The downward movement of the guide tube 8 drives the evenly distributed nozzles to move downward. When the nozzles reach the required height, The electric push rod 10 is turned off by the PLC controller 18, and the infusion pump 11 is operated. The infusion pump 11 pumps the nutrient solution in the storage tank 12 into the inside of the guide tube 8 through the hose, and then injects it into the corresponding culture dish 6 through the nozzle. When the culture dish 6 is filled, the PLC controller 18 turns off the infusion pump 11 and operates the motor 7, moves the filled culture dish 6 out of the injection area, and moves the culture dish 6 to be filled to the injection area. Then the PLC controller 18 operates the infusion pump 11 to fill the nutrient solution, circulates in sequence, and realizes the nutrient solution filling work of all the culture dishes 6. After all the filling is completed, the PLC controller 18 realizes the normal operation of the motor 7.

[0028] It is worth noting that the motor 7 disclosed in the above embodiment can choose a 60M-01330F5-A17 servo motor, the electric push rod 10 can choose a JC35W3 electric telescopic rod, the infusion pump 11 can choose an SB-8 electric liquid pump, the temperature and humidity sensor 13 can choose an OSA temperature and humidity sensor, the heater 14 can choose an XQM-YT1 heating belt, the induced draft fan 16 can choose a 15051 axial flow fan, and the PLC controller 18 can choose a KV-N40DTP programmable controller. The PLC controller 18 controls the motor 7, the electric push rod 10, the infusion pump 11, the temperature and humidity sensor 13, the heater 14 and the induced draft fan 16 using methods commonly used in the prior art.

[0029] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A cell culture device, characterized in that: It comprises a cabinet (1) and an adaptable clamping mechanism (4); Cabinet (1): a rotating column (2) is rotatably connected to the center of the bottom wall thereof, the upper end of the rotating column (2) is fixedly connected to evenly distributed turntables (3), evenly distributed placement grooves (5) are arranged inside the turntables (3), and culture dishes (6) are arranged inside the placement grooves (5), and ventilation holes (15) are arranged at both left and right ends of the cabinet (1); Adaptive clamping mechanisms (4): they are all arranged inside the placement groove (5), and the adaptive clamping mechanisms (4) are all installed in coordination with the vertically adjacent culture dishes (6).

2. A cell culture device according to claim 1, characterized in that: A PLC controller (18) is provided at the right end of the front surface of the cabinet (1), and an input end of the PLC controller (18) is electrically connected to an external power supply.

3. A cell culture device according to claim 1, characterized in that: The adaptable clamping mechanism (4) comprises a fixed groove (41), a telescopic column (42), a spring (43), a clamping plate (44) and an elastic clamp (45); the fixed groove (41) is arranged in the middle of the placement groove (5); circular grooves are arranged at the front and rear ends of the fixed groove (41); a telescopic column (42) is arranged inside the circular groove; the telescopic ends of the telescopic column (42) are fixedly connected to the clamping plate (44); two longitudinally adjacent clamping plates (44) are mounted in cooperation with the lower end of the same culture dish (6); a spring (43) is fixedly connected between the clamping plate (44) and the longitudinally adjacent circular groove; the spring (43) is movably sleeved on the outer surface of the vertically adjacent telescopic column (42); the upper end of the placement groove (5) is provided with symmetrically distributed elastic clamps (45); and two transversely adjacent elastic clamps (45) are mounted in cooperation with the middle of the same culture dish (6).

4. A cell culture device according to claim 2, characterized in that: The front end of the bottom wall of the cabinet (1) is provided with an electric push rod (10), the telescopic end of the electric push rod (10) is fixedly connected to an adjustment frame (9), the upper end of the adjustment frame (9) is provided with a flow guide tube (8), the middle part of the flow guide tube (8) is provided with uniformly distributed nozzles, the nozzles are all installed in cooperation with vertically adjacent culture dishes (6), and the input end of the electric push rod (10) is electrically connected to the output end of the PLC controller (18).

5. A cell culture device according to claim 4, characterized in that: A liquid storage tank (12) is provided at the upper end of the cabinet (1), an infusion pump (11) is provided on the bottom wall of the liquid storage tank (12), a liquid outlet of the infusion pump (11) is connected to the upper end of the guide tube (8) through a hose, a feed pipe is provided at the upper end of the liquid storage tank (12), and an input end of the infusion pump (11) is electrically connected to an output end of a PLC controller (18).

6. A cell culture device according to claim 2, characterized in that: The side walls of the cabinet (1) are provided with symmetrically distributed heaters (14), the front end of the top wall of the cabinet (1) is provided with a temperature and humidity sensor (13), the interior of the right ventilation opening (15) is provided with an induced draft fan (16), the lower end of the cabinet (1) is provided with a motor (7), the output shaft of the motor (7) is fixedly connected to the lower end of the rotating column (2), the input ends of the motor (7), the heater (14) and the induced draft fan (16) are all electrically connected to the output end of a PLC controller (18), and the temperature and humidity sensor (13) is bidirectionally electrically connected to the PLC controller (18).

7. A cell culture device according to claim 1, characterized in that: The front end of the cabinet body (1) is hingedly connected to a cabinet door (17) via a hinge, the middle of the cabinet door (17) is provided with an observation window, and the lower end of the cabinet body (1) is fixedly connected to evenly distributed supporting legs (19), and the lower ends of the supporting legs (19) are all provided with evenly distributed mounting holes.

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