Full-automatic dyeing machine for liquid-based cells

Abstract

The utility model discloses a liquid-based cell full-automatic dyeing machine which comprises a centrifugal machine, the top of the centrifugal machine is rotationally connected with a rotating disc, and a separation assembly is arranged on the rotating disc. The separation assembly comprises a first separation tube, a sample tube, a first reagent tube, a first electric rod, a first piston, a first connecting tube, a second separation tube, a second reagent tube, a second electric rod, a second piston, a liquid outlet tube, a second connecting tube, a waste liquid box, a liquid discharge tube and a glass slide, and the top of the first separation tube is fixedly connected with the sample tube and the first reagent tube; a first electric rod is fixedly connected to the top face of the interior of the first separation pipe, and a first piston is fixedly connected to the bottom of the first electric rod. By means of the structure, all operation devices are integrated together, the structure and operation of the device are simplified, dyeing is conducted through the same step, samples and reagents are more closed, and the pollution risk is greatly reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of medical detection equipment, in particular to a liquid-based cell full-automatic staining machine. Background Art

[0002] In biomedical testing, cells often need to be prepared and stained. The general preparation and staining process includes extracting samples, mixing samples with drug reagents and then centrifuging, discarding the supernatant, transferring the extract to the staining table, adding water to dilute it, pouring out the excess liquid, and staining on the slide.

[0003] The prior art generally divides the preparation and staining into three steps and uses three instruments in sequence to complete them. First, the sample is centrifuged using a centrifuge, and then the supernatant is manually discarded and transferred to a liquid injection device for dilution. After that, the upper liquid is manually poured out and transferred to a staining device for staining. This process is all completed manually, which is not only complicated and difficult to operate, but also has low work efficiency, and waste liquid is difficult to handle, and the probability of sample contamination is high. Utility Model Content

[0004] The purpose of the utility model is to provide a liquid-based cell automatic staining machine, which brings all operating devices together, simplifies the device structure and operation, performs staining in the same steps, and makes the sample and reagent more closed, greatly reducing the risk of contamination. A waste liquid tank is arranged inside the turntable, and the waste liquid can be discharged into the waste liquid tank, reducing the exposure of the waste liquid, facilitating subsequent centralized treatment, and optimizing the function of the equipment.

[0005] To achieve the above-mentioned purpose, a liquid-based cell automatic staining machine is provided, comprising: a centrifuge, wherein the top of the centrifuge is rotatably connected to a turntable, a separation assembly is arranged on the turntable, the separation assembly comprises a first separation tube, a sample tube, a first reagent tube, a first electric rod, a first piston, a first connecting tube, a second separation tube, a second reagent tube, a second electric rod, a second piston, a liquid outlet tube, a second connecting tube, a waste liquid tank, a liquid discharge tube and a glass slide, the top of the first separation tube is fixedly connected to the sample tube and the first reagent tube, the inner top surface of the first separation tube is fixedly connected to the first electric rod, the bottom of the first electric rod is fixedly connected to the first piston, and the The bottom of the first separation tube is fixedly connected to a first connecting tube, the end of the first connecting tube away from the first separation tube is fixedly connected to a second separation tube, the top of the second separation tube is fixedly connected to a second reagent tube, the inner top surface of the second separation tube is fixedly connected to a second electric rod, the bottom of the second electric rod is fixedly connected to a second piston, the bottom of the second separation tube is fixedly connected to a liquid outlet pipe and a second connecting pipe, the bottom of the liquid outlet pipe is fixedly connected to a waste liquid tank, a drain pipe is fixedly connected to the side wall of the waste liquid tank, the top of the turntable is fixedly connected to a glass slide, and the end of the second connecting tube away from the second separation tube is located above the glass slide.

[0006] According to the liquid-based cell automatic staining machine, the waste liquid tank is fixedly connected to the inner circumference of the turntable, and the end of the drain pipe away from the waste liquid tank passes through the turntable.

[0007] According to the liquid-based cell automatic staining machine, valves are arranged on the circumferential surfaces of the first separation tube, sample tube, first reagent tube, first connecting tube, second separation tube, second reagent tube, liquid outlet tube, second connecting tube and liquid discharge tube.

[0008] According to the liquid-based cell automatic staining machine, the sample tube and the bottom of the first reagent tube pass through the first piston, and the end of the first connecting tube away from the first separation tube and the bottom of the second reagent tube both pass through the second piston.

[0009] According to the liquid-based cell automatic staining machine, the top output end of the centrifuge is fixedly connected to the bottom of the waste liquid tank.

[0010] According to the liquid-based cell automatic staining machine, the first separation tube, the first connecting tube, the second separation tube and the second connecting tube all pass through the turntable and are fixedly connected to the turntable.

[0011] According to the liquid-based cell automatic staining machine, the first separation tube, the first connecting tube, the second separation tube and the second connecting tube are all transparent glass tubes.

[0012] The above scheme has the following beneficial effects:

[0013] 1. By arranging a centrifuge, a turntable, a first separation tube, a sample tube, a first reagent tube, a first electric rod, a first piston, a first connecting tube, a second separation tube, a second reagent tube, a second electric rod, a second piston, a liquid outlet tube and a second connecting tube, all operating devices are concentrated together, the device structure and operation are simplified, and staining is performed in the same steps, the sample and the reagent are more closed, and the risk of contamination is greatly reduced;

[0014] 2. By setting up a waste liquid tank and a drain pipe, a waste liquid tank is set inside the turntable, and the waste liquid can be discharged into the waste liquid tank to reduce the exposure of the waste liquid, so as to facilitate subsequent centralized treatment and optimize the function of the equipment.

[0015] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention is further described below with reference to the accompanying drawings and embodiments;

[0017] Figure 1 This is a front view of a liquid-based cell fully automatic staining machine of the utility model;

[0018] Figure 2 This is a structural diagram of the separation components of a liquid-based cell automatic staining machine of the utility model;

[0019] Figure 3 This is a cross-sectional view of the first separation tube of a liquid-based cell automatic staining machine of the utility model;

[0020] Figure 4 This is a cross-sectional view of the second separation tube of a liquid-based cell automatic staining machine of the utility model;

[0021] Figure 5 The utility model is a cross-sectional view of a turntable of a liquid-based cell automatic staining machine.

[0022] Legend:

[0023] 1. Centrifuge; 2. Turntable; 3. First separation tube; 4. Sample tube; 5. First reagent tube; 6. First electric rod; 7. First piston; 8. First connecting tube; 9. Second separation tube; 10. Second reagent tube; 11. Second electric rod; 12. Second piston; 13. Liquid outlet tube; 14. Second connecting tube; 15. Waste liquid tank; 16. Drain tube; 17. Glass slide. DETAILED DESCRIPTION

[0024] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0025] Reference Figure 1-5The utility model embodiment is a liquid-based cell automatic staining machine, which includes: a centrifuge 1, the top of the centrifuge 1 is rotatably connected to a turntable 2, and a separation component is arranged on the turntable 2, the separation component includes a first separation tube 3, a sample tube 4, a first reagent tube 5, a first electric rod 6, a first piston 7, a first connecting tube 8, a second separation tube 9, a second reagent tube 10, a second electric rod 11, a second piston 12, a liquid outlet pipe 13, a second connecting pipe 14, a waste liquid tank 15, a liquid discharge pipe 16 and a slide glass 17, the top of the first separation tube 3 is fixedly connected to the sample tube 4 and the first reagent tube 5, the inner top surface of the first separation tube 3 is fixedly connected to the first electric rod 6, the bottom of the first electric rod 6 is fixedly connected to the first piston 7, the bottoms of the sample tube 4 and the first reagent tube 5 pass through the first piston 7, the first separation tube 3 is fixedly connected to the first reagent tube 5, and the bottoms of the first separation tube 3 are connected to the first reagent tube 5. A first connecting tube 8 is fixedly connected to the bottom of the turntable 2, an end of the first connecting tube 8 away from the first separation tube 3 is fixedly connected to the second separation tube 9, a second reagent tube 10 is fixedly connected to the top of the second separation tube 9, a second electric rod 11 is fixedly connected to the inner top surface of the second separation tube 9, a second piston 12 is fixedly connected to the bottom of the second electric rod 11, an end of the first connecting tube 8 away from the first separation tube 3 and the bottom of the second reagent tube 10 both penetrate the second piston 12, a liquid outlet pipe 13 and a second connecting pipe 14 are fixedly connected to the bottom of the second separation tube 9, a waste liquid tank 15 is fixedly connected to the bottom of the liquid outlet pipe 13, a drain pipe 16 is fixedly connected to the side wall of the waste liquid tank 15, a glass slide 17 is fixedly connected to the top of the turntable 2, and an end of the second connecting tube 14 away from the second separation tube 9 is located above the glass slide 17.

[0026] Valves are provided on the circumferential surfaces of the first separation tube 3, the sample tube 4, the first reagent tube 5, the first connecting tube 8, the second separation tube 9, the second reagent tube 10, the liquid outlet tube 13, the second connecting tube 14 and the liquid discharge tube 16. The first separation tube 3, the first connecting tube 8, the second separation tube 9 and the second connecting tube 14 are all transparent glass tubes for convenient observation and liquid collection.

[0027] The waste liquid tank 15 is fixedly connected to the inner circumference of the turntable 2, the top output end of the centrifuge 1 is fixedly connected to the bottom of the waste liquid tank 15, the end of the discharge pipe 16 away from the waste liquid tank 15 passes through the turntable 2, the first separation tube 3, the first connecting tube 8, the second separation tube 9 and the second connecting tube 14 all pass through the turntable 2 and are fixedly connected to the turntable 2.

[0028] Working principle: add samples and reagents from the sample tube 4 and the first reagent tube 5, start the centrifuge 1 to fully mix the sample in the first separation tube 3 to form upper and lower liquid layers, then start the first electric rod 6 to push the first piston 7 downward, so that the bottom solution in the first separation tube 3 enters the second separation tube 9, then add reagents from the second reagent tube 10, start the centrifuge 1 again, so that the solution in the second separation tube 9 is fully mixed to form upper and lower liquid layers, then start the second electric rod 11 to push the second piston 12 downward, so that the bottom solution in the second separation tube 9 falls onto the slide 17 through the second connecting tube 14 for staining, when the staining is completed, start the first electric rod 6 and the second electric rod 11 to concentrate all waste liquid in the second separation tube 9, then open the liquid outlet pipe 13, so that all waste liquid falls into the waste liquid tank 15, and is discharged through the drain pipe 16.

[0029] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A liquid-based cell automatic staining machine, comprising: A centrifuge (1), characterized in that the top of the centrifuge (1) is rotatably connected to a turntable (2), and a separation assembly is arranged on the turntable (2), wherein the separation assembly comprises a first separation tube (3), a sample tube (4), a first reagent tube (5), a first electric rod (6), a first piston (7), a first connecting tube (8), a second separation tube (9), a second reagent tube (10), a second electric rod (11), a second piston (12), a liquid outlet pipe (13), a second connecting pipe (14), a waste liquid tank (15), a liquid discharge pipe (16) and a glass slide (17), wherein the top of the first separation tube (3) is fixedly connected to the sample tube (4) and the first reagent tube (5), the inner top surface of the first separation tube (3) is fixedly connected to the first electric rod (6), the bottom of the first electric rod (6) is fixedly connected to the first piston (7), and the first separation tube (3) is fixedly connected to the bottom of the first electric rod (6). ) is fixedly connected to a first connecting tube (8) at the bottom, one end of the first connecting tube (8) away from the first separation tube (3) is fixedly connected to a second separation tube (9), the top of the second separation tube (9) is fixedly connected to a second reagent tube (10), the inner top surface of the second separation tube (9) is fixedly connected to a second electric rod (11), the bottom of the second electric rod (11) is fixedly connected to a second piston (12), the bottom of the second separation tube (9) is fixedly connected to a liquid outlet pipe (13) and a second connecting tube (14), the bottom of the liquid outlet pipe (13) is fixedly connected to a waste liquid tank (15), a liquid drain pipe (16) is fixedly connected to the side wall of the waste liquid tank (15), a glass slide (17) is fixedly connected to the top of the turntable (2), and the end of the second connecting tube (14) away from the second separation tube (9) is located above the glass slide (17).

2. A liquid-based cell automatic staining machine according to claim 1, characterized in that: The waste liquid tank (15) is fixedly connected to the inner circumferential surface of the turntable (2), and the end of the liquid discharge pipe (16) away from the waste liquid tank (15) passes through the turntable (2).

3. A liquid-based cell automatic staining machine according to claim 1, characterized in that: Valves are provided on the circumferential surfaces of the first separation tube (3), the sample tube (4), the first reagent tube (5), the first connecting tube (8), the second separation tube (9), the second reagent tube (10), the liquid outlet tube (13), the second connecting tube (14) and the liquid discharge tube (16).

4. A liquid-based cell automatic staining machine according to claim 1, characterized in that: The bottoms of the sample tube (4) and the first reagent tube (5) penetrate the first piston (7), and the end of the first connecting tube (8) away from the first separation tube (3) and the bottom of the second reagent tube (10) both penetrate the second piston (12).

5. A liquid-based cell automatic staining machine according to claim 1, characterized in that: The top output end of the centrifuge (1) is fixedly connected to the bottom of the waste liquid tank (15).

6. A liquid-based cell automatic staining machine according to claim 1, characterized in that: The first separation tube (3), the first connecting tube (8), the second separation tube (9) and the second connecting tube (14) all pass through the rotating disk (2) and are fixedly connected to the rotating disk (2).

7. A liquid-based cell automatic staining machine according to claim 1, characterized in that: The first separation tube (3), the first connecting tube (8), the second separation tube (9) and the second connecting tube (14) are all transparent glass tubes.

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