Cell screening device for stem cells

Abstract

The utility model discloses a cell screening device for stem cells, which relates to the related technical field of cell screening devices and comprises a scattering cylinder, a stirring motor, a stirring rod, a filter screen, a centrifugal cylinder and a collecting cylinder. A sealing cover is arranged on the scattering barrel, a cell liquid inlet and a physiological water inlet are formed in the sealing cover, the stirring rod is vertically arranged in the scattering barrel, the stirring motor is arranged on the sealing cover, and the output end of the stirring motor penetrates through the sealing cover and is arranged on the stirring rod; the lower end of the scattering barrel is in a funnel shape, the bottom of the scattering barrel is communicated with a liquid discharging pipe, a valve plate is arranged on the liquid discharging pipe, the filter screen is arranged at the bottom of the liquid discharging pipe, the filter screen is located on the upper portion of the interior of the centrifugal barrel, and the centrifugal barrel is arranged in the collecting barrel in a sleeved mode; the cell screening device can automatically screen out stem cells, and the filtering and separating efficiency of the stem cells is improved.

Description

Technical Field

[0001] The utility model relates to the technical field related to cell screening devices, in particular to a cell screening device for stem cells. Background Art

[0002] Stem cells are a type of cells with the potential for self-renewal and differentiation. After a long period of medical research and development, stem cells have been increasingly used. However, the extraction of stem cells has always been done by sampling from living bodies. Therefore, the samples taken out will certainly not only contain stem cells. Therefore, the screening of stem cells is particularly important. Although there are many different screening devices, the screening environment and the hygiene of the screening devices are still not rigorous enough.

[0003] After the cells proliferate, the sizes of the cells are different, so the cell clusters in the separated cell fluid are of different sizes, which may affect the research on stem cell differentiation;

[0004] In summary, there is an urgent need for a stem cell screening device to solve the above problems. Utility Model Content

[0005] The utility model aims to provide a cell screening device for stem cells, which can automatically screen out stem cells and improve the filtering and separation efficiency of stem cells.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell screening device for stem cells, comprising a spreading cylinder, a stirring motor, a stirring rod, a filter, a centrifugal cylinder, and a collecting cylinder; a sealing cover is provided on the spreading cylinder, a cell liquid inlet and a physiological water inlet are provided on the sealing cover, the stirring rod is vertically arranged in the spreading cylinder, the stirring motor is arranged on the sealing cover, and the output end of the stirring motor passes through the sealing cover and is arranged on the stirring rod; the lower end of the spreading cylinder is funnel-shaped, and the bottom of the spreading cylinder is connected to a drain pipe, a valve plate is provided on the drain pipe, the filter is arranged at the bottom of the drain pipe, the filter is located above the inside of the centrifugal cylinder, and the centrifugal cylinder is sleeved in the collecting cylinder.

[0007] In order to prevent cells from being contaminated, as a preferred stem cell screening device of the utility model, the cell liquid inlet and the physiological water inlet are provided with connecting covers, and sterilizing lamps are embedded on the inner walls of the cell liquid inlet and the physiological water inlet and on the bottom of the sealing cover.

[0008] In order to facilitate the disassembly and assembly of the filter screen, as a preferred cell screening device for stem cells of the utility model, the filter screen includes a filter screen layer and a support sleeve arranged on the edge of the filter screen layer, a thread groove of an annular structure is opened at the bottom of the drain pipe, and the top thread of the support sleeve is arranged in the thread groove.

[0009] In order to facilitate the screening of stem cells, a stem cell screening device of the utility model is preferably provided with a holder inside the collecting tube, a rotating motor is provided at the bottom inside the collecting tube, the output end of the rotating motor is provided at the bottom of the holder, a sleeve matching the holder is provided at the bottom of the centrifugal tube, and the holder is sleeved in the sleeve.

[0010] In order to prevent liquid from splashing outside the collecting tube, as a preferred cell screening device for stem cells of the utility model, a connecting sleeve is provided on the outer wall of the discharge pipe, the connecting sleeve is located below the valve plate, and the upper part of the outer wall of the collecting tube is threadedly connected to the inner wall of the connecting sleeve; a limiting groove is provided at the bottom of the connecting sleeve, and the top of the centrifugal tube is sleeved in the limiting groove.

[0011] In order to enhance the stability of the scattering barrel, as a preferred stem cell screening device of the utility model, the scattering barrel is provided with a support frame, a counterweight base is provided at the bottom of the support frame, and the collecting barrel is located above the counterweight base.

[0012] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0013] The utility model comprises a scattering cylinder, a stirring motor, a stirring rod, a filter screen, a centrifugal cylinder and a collecting cylinder. By starting the stirring motor, the stirring rod rotates, the stirring rod can evenly mix the cell fluid and the physiological water, and can also scatter the cells to separate the cells. The valve plate on the discharge pipe is opened, and the liquid in the scattering cylinder flows downward, the filter screen plays a filtering role, so that larger tissues and impurities cannot pass through the filter screen, and the liquid flows into the centrifugal cylinder. When the centrifugal cylinder rotates, small cells can be separated from the centrifugal cylinder, and the collecting cylinder plays a role in collecting liquid and small cells, so that the cell clusters in the separated cell fluid are of the same size, which will not affect the research on stem cell differentiation. The cell screening device can automatically screen out the stem cells, greatly improving the stem cell filtering and separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of the utility model;

[0015] Figure 2 for Figure 1 AA structural diagram;

[0016] Figure 3 It is a schematic cross-sectional view of the physiological water inlet of the utility model.

[0017] In the figure: 1. Sprinkling cylinder; 2. Stirring motor; 3. Stirring rod; 4. Filter screen; 5. Centrifugal cylinder; 6. Collecting cylinder; 7. Sealing cover; 8. Cell inlet; 9. Physiological water inlet; 10. Drain pipe; 11. Valve plate; 12. Connecting cover; 13. Sterilization lamp; 14. Filter screen layer; 15. Support sleeve; 16. Threaded groove; 17. Clamping seat; 18. Rotating motor; 19. Clamping sleeve; 20. Connecting sleeve; 21. Limiting groove; 22. Support frame; 23. Counterweight base. DETAILED DESCRIPTION

[0018] See also Figures 1 to 3 A cell screening device for stem cells comprises a spreading cylinder 1, a stirring motor 2, a stirring rod 3, a filter screen 4, a centrifugal cylinder 5 and a collecting cylinder 6; a sealing cover 7 is provided on the spreading cylinder 1, a cell inlet 8 and a physiological water inlet 9 are provided on the sealing cover 7, the stirring rod 3 is vertically arranged in the spreading cylinder 1, the stirring motor 2 is arranged on the sealing cover 7, and the output end of the stirring motor 2 passes through the sealing cover 7 and is arranged on the stirring rod 3; the lower end of the spreading cylinder 1 is funnel-shaped, and a drain pipe 10 is connected to the bottom of the spreading cylinder 1, a valve plate 11 is provided on the drain pipe 10, the filter screen 4 is arranged at the bottom of the drain pipe 10, the filter screen 4 is located above the filter screen 4 inside the centrifugal cylinder 5, and the centrifugal cylinder 5 is sleeved in the collecting cylinder 6.

[0019] In this embodiment, the cell fluid and the physiological saline are respectively transported to the spreading cylinder 1 through the cell fluid inlet and the physiological water inlet 9, the stirring motor 2 is started, and the stirring rod 3 is rotated. The stirring rod 3 can make the cell fluid and the physiological water evenly mixed together, and at the same time can also break up the cells and separate the cells. The valve plate 11 on the discharge pipe 10 is opened, and the liquid in the spreading cylinder 1 flows downward. The filter screen 4 plays a filtering role, so that larger tissues and impurities cannot pass through the filter screen 4. The liquid flows into the centrifugal cylinder 5. When the centrifugal cylinder 5 rotates, small cells can be separated from the centrifugal cylinder 5. The collecting cylinder 6 plays the role of collecting liquid and small cells, so that the cell clusters in the separated cell fluid are of the same size, which will not affect the research on stem cell differentiation. The cell screening device can automatically screen out the stem cells, which greatly improves the efficiency of stem cell filtration and separation.

[0020] As a technical optimization solution of the utility model, a connecting cover 12 is provided on the cell inlet 8 and the physiological water inlet 9, and a sterilizing lamp 13 is embedded on the inner walls of the cell inlet 8 and the physiological water inlet 9 and the bottom of the sealing cover 7.

[0021] In this embodiment: the connecting cover 12 enhances the sealing performance of the cell liquid inlet 8 and the physiological water inlet 9 to prevent the entry of other foreign matter, and the sterilization lamp 13 is turned on to sterilize the cell liquid inlet 8, the physiological water inlet 9, and the spreading tube 1 to prevent the cells from being contaminated.

[0022] As a technical optimization solution of the utility model, the filter screen 4 includes a filter screen layer 14 and a support sleeve 15 arranged on the edge of the filter screen layer 14. A thread groove 16 of an annular structure is opened at the bottom of the drain pipe 10, and the top thread of the support sleeve 15 is arranged in the thread groove 16.

[0023] In this embodiment, the support sleeve 15 is threadedly installed in the thread groove 16, so that the filter screen 4 can be easily disassembled, replaced and sterilized and cleaned.

[0024] As a technical optimization solution of the utility model, a holder 17 is arranged inside the collecting cylinder 6, a rotating motor 18 is arranged at the bottom inside the collecting cylinder 6, the output end of the rotating motor 18 is arranged at the bottom of the holder 17, a sleeve 19 adapted to the holder 17 is arranged at the bottom of the centrifugal cylinder 5, and the holder 17 is sleeved in the sleeve 19.

[0025] In this embodiment, the ferrule 19 below the centrifugal cylinder 5 is sleeved on the holder 17. When the rotary motor 18 is started, the holder 17 can drive the centrifugal cylinder 5 to rotate, which is easy to screen cells. The holder 17 has a polygonal structure.

[0026] As a technical optimization solution of the utility model, a connecting sleeve 20 is provided on the outer wall of the discharge pipe 10, and the connecting sleeve 20 is located below the valve plate 11. The upper part of the outer wall of the collecting cylinder 6 is threadedly connected to the inner wall of the connecting sleeve 20; a limiting groove 21 is provided at the bottom of the connecting sleeve 20, and the top of the centrifugal cylinder 5 is sleeved in the limiting groove 21.

[0027] In this embodiment: the thread on the collecting cylinder 6 is installed in the connecting sleeve 20, and the connecting sleeve 20 plays a sealing role to prevent the liquid from splashing to the outside of the collecting cylinder 6 during centrifugal separation; and the upper end of the centrifugal cylinder 5 is sleeved in the limiting groove 21, and the stability of the rotation of the centrifugal cylinder 5 can be enhanced when the centrifugal cylinder 5 is rotated to separate cells.

[0028] As a technical optimization solution of the utility model, a support frame 22 is provided on the scattering barrel 1 , a counterweight base 23 is provided at the bottom of the support frame 22 , and the collecting barrel 6 is located above the counterweight base 23 .

[0029] In this embodiment, the support frame 22 supports and fixes the spreading cylinder 1 , and the counterweight base 23 enhances the stability of the spreading cylinder 1 to prevent the spreading cylinder 1 from shaking or tipping over.

[0030] Working principle: First, the connecting sleeve 20 at the bottom of the centrifugal cylinder 5 is sleeved on the seat 17 in the collecting cylinder 6, and the collecting cylinder 6 is threadedly installed in the connecting sleeve 20. During installation, the upper end of the centrifugal cylinder 5 is sleeved in the limiting groove 21; then the valve plate 11 is closed, and the cell fluid and the physiological saline are respectively transported to the spreading cylinder 1 through the cell fluid inlet and the physiological water inlet 9, and the stirring motor 2 is started, and the stirring rod 3 is rotated. The stirring rod 3 can make the cell fluid and the physiological water evenly mixed together, and at the same time, the cells can be scattered and separated. Then the valve plate 11 on the discharge pipe 10 is opened, and the liquid in the spreading cylinder 1 flows downward, and the filter screen 4 plays a filtering role, so that larger tissues and impurities cannot pass through the filter screen 4, and the liquid flows into the centrifugal cylinder 5. When the centrifugal cylinder 5 rotates, small cells can be separated from the centrifugal cylinder 5, and the collecting cylinder 6 plays a role in collecting liquid and small cells, so that the cell groups in the separated cell fluid are of the same size, which will not affect the research on stem cell differentiation. The cell screening device can automatically screen out the stem cells, which greatly improves the filtration and separation efficiency of stem cells.

[0031] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell screening device for stem cells, characterized in that: The invention comprises a spreading cylinder (1), a stirring motor (2), a stirring rod (3), a filter (4), a centrifugal cylinder (5), and a collecting cylinder (6); the spreading cylinder (1) is provided with a sealing cover (7), the sealing cover (7) is provided with a cell liquid inlet (8) and a physiological water inlet (9), the stirring rod (3) is vertically arranged in the spreading cylinder (1), the stirring motor (2) is arranged on the sealing cover (7), and the output end of the stirring motor (2) penetrates the sealing cover (7) and is arranged on the stirring rod (3); the lower end of the spreading cylinder (1) is funnel-shaped, and the bottom of the spreading cylinder (1) is connected to a drainage pipe (10), the drainage pipe (10) is provided with a valve plate (11), the filter (4) is arranged at the bottom of the drainage pipe (10), the filter (4) is located above the inside of the centrifugal cylinder (5), and the centrifugal cylinder (5) is sleeved in the collecting cylinder (6).

2. A stem cell screening device according to claim 1, characterized in that: The cell liquid inlet (8) and the physiological water inlet (9) are provided with a connecting cover (12), and the inner walls of the cell liquid inlet (8) and the physiological water inlet (9) and the bottom of the sealing cover (7) are embedded with a sterilizing lamp (13).

3. The stem cell screening device according to claim 1, characterized in that: The filter screen (4) comprises a filter screen layer (14) and a support sleeve (15) arranged on the edge of the filter screen layer (14); a thread groove (16) of an annular structure is provided at the bottom of the drain pipe (10); and a top of the support sleeve (15) is threadedly arranged in the thread groove (16).

4. The stem cell screening device according to claim 1, characterized in that: A holder (17) is arranged inside the collecting cylinder (6), a rotating motor (18) is arranged at the bottom of the collecting cylinder (6), an output end of the rotating motor (18) is arranged at the bottom of the holder (17), a sleeve (19) adapted to the holder (17) is arranged at the bottom of the centrifugal cylinder (5), and the holder (17) is sleeved in the sleeve (19).

5. The stem cell screening device according to claim 1, characterized in that: A connecting sleeve (20) is provided on the outer wall of the liquid discharge pipe (10), the connecting sleeve (20) is located below the valve plate (11), and the upper part of the outer wall of the collecting cylinder (6) is threadedly connected to the inner wall of the connecting sleeve (20); a limiting groove (21) is provided at the bottom of the connecting sleeve (20), and the top of the centrifugal cylinder (5) is sleeved in the limiting groove (21).

6. The stem cell screening device according to claim 1, characterized in that: A support frame (22) is provided on the scattering cylinder (1), a counterweight base (23) is provided at the bottom of the support frame (22), and the collecting cylinder (6) is located above the counterweight base (23).

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