Magnetic stirring device and bioreactor

Abstract

The utility model relates to a magnetic stirring device which comprises a driving assembly and a stirring assembly, the stirring assembly comprises a magnetic assembly, a bearing seat assembly, a stirring shaft and a connecting piece; wherein the connecting piece comprises a driving connecting part, a cover plate connecting part, a lower magnetic cylinder connecting part, an oil seal connecting part, a bearing connecting part and a stirring shaft connecting part which are sequentially arranged from top to bottom, and a mounting hole is formed in the upper end of the connecting piece; the mounting hole penetrates through the driving connecting part and the cover plate connecting part in the axial direction of the connecting piece and extends into the lower magnetic cylinder connecting part; the magnetic component comprises an upper magnetic cylinder unit and a lower magnetic cylinder unit, the upper end of the upper magnetic cylinder unit is connected with the output shaft of the driving component, and the lower end of the upper magnetic cylinder unit is inserted into the mounting hole of the connecting piece. Cleanness can be guaranteed, cell pollution is avoided, and the service life is longer.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a magnetic stirring device and a bioreactor. Background Art

[0002] There are some problems with traditional stirring devices during use. On the one hand, due to the long-term impact and wear of the stirring shaft by the material, fatigue fracture or other damage problems are prone to occur. On the other hand, the upper and lower magnetic cylinders of the stirring device are externally placed, which easily produces other substances that affect the cultivation accuracy of the cultured materials in the tank. Therefore, a magnetic stirring device is proposed to address the above problems. Utility Model Content

[0003] The utility model aims to overcome the existing defects and provide a magnetic stirring device, which can ensure cleanliness, avoid cell contamination and have a longer service life.

[0004] The technical solution to achieve the above object is: a magnetic stirring device, comprising: a driving component and a stirring component;

[0005] The stirring assembly includes a magnetic assembly, a bearing seat assembly, a stirring shaft and a connecting piece;

[0006] The connecting member includes a driving connecting portion, a cover connecting portion, a lower magnetic cylinder connecting portion, an oil seal connecting portion, a bearing connecting portion and a stirring shaft connecting portion which are sequentially arranged from top to bottom, and a mounting hole is provided at the upper end of the connecting member, and the mounting hole penetrates the driving connecting portion and the cover connecting portion along the axial direction of the connecting member and extends to the lower magnetic cylinder connecting portion;

[0007] The magnetic assembly includes an upper magnetic cylinder unit and a lower magnetic cylinder unit, the upper end of the upper magnetic cylinder unit is connected to the output shaft of the driving assembly, the lower end of the upper magnetic cylinder unit is inserted into the mounting hole of the connecting piece, the lower magnetic cylinder unit is sleeved on the lower magnetic cylinder connecting part of the connecting piece, the bearing seat assembly is sleeved on the bearing connecting part of the connecting piece, and the upper end of the bearing seat assembly is connected to the lower end of the lower magnetic cylinder unit, and the lower end of the bearing seat assembly is connected to the upper end of the stirring shaft.

[0008] Preferably, the upper magnetic cylinder unit includes a first magnet base and a plurality of first permanent magnets, and the plurality of first permanent magnets are fixed in mounting grooves opened at the lower end of the first magnet base; the lower magnetic cylinder unit includes a second magnet base and a plurality of second permanent magnets, and the plurality of second permanent magnets are fixed in mounting grooves opened at the upper end of the second magnet base.

[0009] Preferably, a plurality of the first permanent magnets are evenly arranged along the circumference of the first magnet base, and the N poles and S poles of two adjacent first permanent magnets are staggered; a plurality of the second permanent magnets are evenly arranged along the circumference of the second magnet base, and the N poles and S poles of two adjacent second permanent magnets are staggered.

[0010] Preferably, the drive assembly includes a servo motor, which is connected to a motor seat, and the motor seat is connected to the driving connection part of the connecting member. A first connecting groove is provided on the magnet seat of the upper magnetic cylinder unit, and the first connecting groove is used to cooperate with the output shaft of the servo motor.

[0011] Preferably, the bearing seat assembly comprises: a bearing, a limiting ring, a bearing seat and an oil seal;

[0012] The limiting ring is located between the two bearings, the limiting ring and the two bearings are both sleeved on the bearing connecting portion of the connecting piece, the bearing seat is sleeved on the outer sides of the two bearings, the upper end surface of the bearing seat is connected to the lower end surface of the lower magnetic cylinder unit, the lower end surface of the bearing seat is connected to the upper end surface of the stirring shaft, the oil seal is sleeved on the oil seal connecting portion of the connecting piece, and the upper end surface of the bearing seat is provided with an annular boss, and the annular boss is used to press and fix the oil seal.

[0013] Preferably, a first sealing ring is provided between the upper end surface of the bearing seat and the lower end surface of the lower magnetic cylinder unit;

[0014] A second sealing ring is arranged between the lower end surface of the bearing seat and the upper end surface of the stirring shaft.

[0015] Preferably, it further comprises a limiting nut and a fixing screw, the lower end of the connecting piece is provided with a threaded structure, the limiting nut is connected to the lower end of the connecting piece, and the fixing screw cooperates with the limiting nut to fix the bearing on the connecting piece.

[0016] Preferably, the connecting member is connected to the cover plate of the culture tank by bolts, and the size of the cover plate connecting portion is set according to the diameter of the through hole reserved on the cover plate.

[0017] Preferably, it also includes a paddle, wherein the paddle is mounted on the stirring shaft.

[0018] Accordingly, the utility model also provides a bioreactor, comprising: a culture tank and the magnetic stirring device as described above;

[0019] The culture tank comprises a tank body and a cover plate, wherein the cover plate is provided with a through hole, the stirring shaft of the magnetic stirring device extends into the tank body through the through hole, and the connecting piece of the magnetic stirring device is fixedly connected to the cover plate through a cover plate connecting portion.

[0020] The beneficial effects of the utility model are as follows: a stirring shaft is arranged to be fixed in the reactor, stirring is driven by magnetic force, and a connecting piece with a specific structure is designed to assemble a driving motor, an upper magnetic cylinder, a lower magnetic cylinder, a bearing and a stirring shaft together; when the motor drives the upper magnetic cylinder to rotate, the lower magnetic cylinder and the bearing seat fixed thereto and the stirring shaft rotate together; since the upper magnetic cylinder unit is wrapped by the connecting piece, foreign matter generated when the upper magnetic cylinder unit rotates will not enter the tank body; at the same time, since the lower magnetic cylinder unit and the bearing seat wrap the skeleton oil seal, the bearing and other components inside, impurities generated by these components will not enter the tank body; the magnetic stirring device not only has a low failure rate and a long service life, but also can prevent impurities from entering the tank body and contaminating cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the magnetic stirring device of the utility model;

[0022] Figure 2 It is a cross-sectional view of the magnetic stirring device of the utility model;

[0023] Figure 3 It is a cross-sectional view of the upper magnetic cylinder unit of the utility model;

[0024] Figure 4 It is a cross-sectional view of the lower magnetic cylinder unit of the utility model;

[0025] Figure 5 It is a cross-sectional view of the bearing seat of the utility model;

[0026] Figure 6 It is a cross-sectional view of the connector of the utility model;

[0027] Figure 7 It is a schematic diagram of the stirring assembly of the utility model.

[0028] In the figure: 1. limit nut; 2. stirring shaft; 3. bearing; 4. limit ring; 5. bearing seat; 6. oil seal; 7. lower magnetic cylinder unit; 8. connecting piece; 9. upper magnetic cylinder unit; 10. bolt; 11. motor seat; 12. servo motor; 13. sealing ring; 14. set screw. DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0030] The utility model will be further described below in conjunction with the accompanying drawings.

[0031] like Figure 1-7 A magnetic stirring device is shown, comprising: a driving assembly and a stirring assembly; the stirring assembly comprises a magnetic assembly, a bearing seat assembly, a stirring shaft 2 and a connecting member 8; wherein the connecting member 8 comprises a driving connecting portion 82, a cover connecting portion 83, a lower magnetic cylinder connecting portion 84, an oil seal connecting portion 85, a bearing connecting portion 86 and a stirring shaft connecting portion 87 which are arranged in sequence from top to bottom, and a mounting hole 81 is opened at the upper end of the connecting member 8, and the mounting hole 81 penetrates the driving connecting portion 82 and the cover connecting portion 83 along the axial direction of the connecting member 8 And extends to the lower magnetic cylinder connecting part 84; the magnetic assembly includes an upper magnetic cylinder unit 9 and a lower magnetic cylinder unit 7, the upper end of the upper magnetic cylinder unit 9 is connected to the output shaft of the driving assembly, the lower end of the upper magnetic cylinder unit 9 is inserted into the mounting hole 81 of the connecting member 8, the lower magnetic cylinder unit 7 is sleeved on the lower magnetic cylinder connecting part 84 of the connecting member 8, the bearing seat assembly is sleeved on the bearing connecting part 86 of the connecting member 8, and the upper end of the bearing seat assembly is connected to the lower end of the lower magnetic cylinder unit 7, and the lower end of the bearing seat assembly is connected to the upper end of the stirring shaft 2.

[0032] Specifically, both the upper magnetic cylinder unit 9 and the lower magnetic cylinder unit 7 use permanent magnets, and the permanent magnets of each component are fixed by a magnet base, that is, the permanent magnets are installed in a magnet base, and the magnet base of the upper magnetic cylinder unit 9 is matched and connected with the output shaft / stirring shaft of the servo motor 12. The upper magnetic cylinder unit 9 drives the lower magnetic cylinder unit 7 to rotate by magnetic coupling, and a gap is left between the upper magnetic cylinder unit 9 and the lower magnetic cylinder unit 7 to avoid affecting the induction of magnetic force.

[0033] like Figure 3 As shown, the upper magnetic cylinder unit 9 includes a first magnet base and a plurality of first permanent magnets 9a, and the plurality of first permanent magnets 9a are fixed in the mounting grooves opened at the lower end of the first magnet base; Figure 4 As shown, the lower magnetic cylinder unit 7 includes a second magnet base and a plurality of second permanent magnets 7a, and the plurality of second permanent magnets 7a are fixed in mounting grooves opened at the upper end of the second magnet base.

[0034] Preferably, a plurality of first permanent magnets 9a are evenly arranged along the circumference of the first magnet base, and the N poles and S poles of two adjacent first permanent magnets 9a are alternately arranged.

[0035] Preferably, a plurality of second permanent magnets 7a are evenly arranged along the circumference of the second magnet base, and the N poles and S poles of two adjacent second permanent magnets 7a are alternately arranged.

[0036] Please refer to Figure 2 , Figure 3 and Figure 6 The driving assembly includes a servo motor 12, which is connected to a motor base 11. The motor base 11 is connected to a driving connection portion 82 of a connecting member 8. A first connecting groove is provided on a magnet base of an upper magnetic cylinder unit 9, and the first connecting groove is used to cooperate with an output shaft of the servo motor 12.

[0037] Please refer to Figure 2 , Figure 5 and Figure 6 The bearing seat assembly includes: a bearing 3, a limiting ring 4, a bearing seat 5 and an oil seal 6; the limiting ring 4 is located between the two bearings 3, the limiting ring 4 and the two bearings 3 are both sleeved on the bearing connecting portion 86 of the connecting piece 8, the bearing seat 5 is sleeved on the outer sides of the two bearings 3, the upper end surface of the bearing seat 5 is connected to the lower end surface of the lower magnetic cylinder unit 7, the lower end surface of the bearing seat 5 is connected to the upper end surface of the stirring shaft 2, the oil seal 6 is sleeved on the oil seal connecting portion 85 of the connecting piece 8, and the upper end surface of the bearing seat 5 is provided with an annular boss, which is used to press and fix the oil seal 6.

[0038] Specifically, the bearing 3 is installed in the inner space of the bearing seat 5, and the limiting ring 4 is selected to locate the middle of the bearing 3, and the TC skeleton oil seal 6 is selected to ensure that the bearing 3 can run stably. A first sealing ring 13 is arranged between the upper end surface of the bearing seat 5 and the lower end surface of the lower magnetic cylinder unit 7; a second sealing ring 16 is arranged between the lower end surface of the bearing seat 5 and the upper end surface of the stirring shaft 2.

[0039] Please continue to refer to Figure 2 The magnetic stirring device also includes a limit nut 1 and a set screw 14. The lower end of the connecting piece 8 is provided with a threaded structure. The limit nut 1 is connected to the lower end of the connecting piece 8. The set screw 14 cooperates with the limit nut 1 to fix the bearing 3 on the connecting piece 8. During the stirring process, the limit nut 1 can effectively prevent the force generated by the stirring material from affecting the stirring shaft and causing the stirring shaft to shake.

[0040] In this embodiment, the magnetic stirring device further comprises a plurality of blades, and the blades are mounted on the stirring shaft 2. Preferably, the plurality of blades are detachably mounted on the stirring shaft 2. Further, the plurality of blades are divided into two groups, which are respectively arranged at the upper and lower positions.

[0041] Correspondingly, the present application also provides a bioreactor, comprising: a culture tank and the magnetic stirring device as described above; the culture tank comprises a tank body and a cover plate, the cover plate is provided with a through hole, the stirring shaft 2 of the magnetic stirring device extends into the tank body through the through hole, and the connecting piece 8 of the magnetic stirring device is fixedly connected to the cover plate through the cover plate connecting part 83.

[0042] Specifically, Figure 2 As shown, the size of the cover plate connecting part 83 is set according to the diameter of the through hole reserved on the cover plate, and a bolt hole is opened on the cover plate connecting part 83. The bolt 10 is set in the bolt hole. The cover plate connecting part 83 is connected to the cover plate of the culture tank by bolt connection.

[0043] Please refer to the above for the magnetic stirring device, which will not be described here. When the motor drives the upper magnetic cylinder unit 9 to rotate, the lower magnetic cylinder unit 7, the bearing seat 5 and the stirring shaft 2 rotate together. Since the upper magnetic cylinder unit 9 is wrapped by the connecting piece 8, the foreign matter generated when the upper magnetic cylinder unit 9 rotates will not enter the tank body to contaminate the cells. At the same time, since the lower magnetic cylinder unit 7 and the bearing seat 5 wrap the skeleton oil seal 6, the bearing 3 and other components inside, the impurities generated by these components will not enter the tank body to contaminate the cells.

[0044] The above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model is described in detail with reference to the above embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the above embodiments, or replace some or all of the technical features therein with equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model.

Claims

1. A magnetic stirring device, characterized in that: include: Driving assembly and stirring assembly; The stirring assembly comprises a magnetic assembly, a bearing seat assembly, a stirring shaft (2) and a connecting piece (8); The connecting member (8) comprises a driving connecting portion (82), a cover connecting portion (83), a lower magnetic cylinder connecting portion (84), an oil seal connecting portion (85), a bearing connecting portion (86) and a stirring shaft connecting portion (87) which are arranged in sequence from top to bottom. A mounting hole (81) is provided at the upper end of the connecting member (8). The mounting hole (81) penetrates the driving connecting portion (82) and the cover connecting portion (83) along the axial direction of the connecting member (8) and extends to the lower magnetic cylinder connecting portion (84). The magnetic assembly comprises an upper magnetic cylinder unit (9) and a lower magnetic cylinder unit (7), the upper end of the upper magnetic cylinder unit (9) is connected to the output shaft of the driving assembly, the lower end of the upper magnetic cylinder unit (9) is inserted into the mounting hole (81) of the connecting member (8), the lower magnetic cylinder unit (7) is sleeved on the lower magnetic cylinder connecting portion (84) of the connecting member (8), the bearing seat assembly is sleeved on the bearing connecting portion (86) of the connecting member (8), and the upper end of the bearing seat assembly is connected to the lower end of the lower magnetic cylinder unit (7), and the lower end of the bearing seat assembly is connected to the upper end of the stirring shaft (2).

2. The magnetic stirring device according to claim 1, characterized in that: The upper magnetic cylinder unit (9) comprises a first magnet base and a plurality of first permanent magnets (9a), wherein the plurality of first permanent magnets (9a) are fixed in mounting grooves opened at the lower end of the first magnet base; the lower magnetic cylinder unit (7) comprises a second magnet base and a plurality of second permanent magnets (7a), wherein the plurality of second permanent magnets (7a) are fixed in mounting grooves opened at the upper end of the second magnet base.

3. The magnetic stirring device according to claim 2, characterized in that: A plurality of the first permanent magnets (9a) are evenly arranged along the circumference of the first magnet base, and the N poles and S poles of two adjacent first permanent magnets (9a) are arranged alternately; a plurality of the second permanent magnets (7a) are evenly arranged along the circumference of the second magnet base, and the N poles and S poles of two adjacent second permanent magnets (7a) are arranged alternately.

4. The magnetic stirring device according to claim 2, characterized in that: The drive assembly comprises a servo motor (12), the servo motor (12) is connected to a motor base (11), the motor base (11) is connected to a drive connection portion (82) of the connecting member (8), and a first connection groove is provided on the magnet base of the upper magnetic cylinder unit (9), the first connection groove is used to cooperate with and connect the output shaft of the servo motor (12).

5. The magnetic stirring device according to claim 1, characterized in that: The bearing seat assembly comprises: a bearing (3), a limiting ring (4), a bearing seat (5) and an oil seal (6); The limiting ring (4) is located between the two bearings (3). The limiting ring (4) and the two bearings (3) are both sleeved on the bearing connection part (86) of the connecting member (8). The bearing seat (5) is sleeved on the outer sides of the two bearings (3). The upper end surface of the bearing seat (5) is connected to the lower end surface of the lower magnetic cylinder unit (7). The lower end surface of the bearing seat (5) is connected to the upper end surface of the stirring shaft (2). The oil seal (6) is sleeved on the oil seal connection part (85) of the connecting member (8). The upper end surface of the bearing seat (5) is provided with an annular boss, and the annular boss is used to press and fix the oil seal (6).

6. The magnetic stirring device according to claim 5, characterized in that: A first sealing ring (13) is provided between the upper end surface of the bearing seat (5) and the lower end surface of the lower magnetic cylinder unit (7); A second sealing ring (16) is provided between the lower end surface of the bearing seat (5) and the upper end surface of the stirring shaft (2).

7. The magnetic stirring device according to claim 5, characterized in that: It also includes a limiting nut (1) and a set screw (14); the lower end of the connecting piece (8) is provided with a threaded structure; the limiting nut (1) is connected to the lower end of the connecting piece (8); the set screw (14) cooperates with the limiting nut (1) to fix the bearing (3) on the connecting piece (8).

8. The magnetic stirring device according to claim 1, characterized in that: The connecting piece (8) is connected to the cover plate of the culture tank via bolts (10), and the size of the cover plate connecting portion (83) is set according to the diameter of the through hole reserved on the cover plate.

9. The magnetic stirring device according to claim 1, characterized in that: It also comprises a paddle, wherein the paddle is mounted on the stirring shaft (2).

10. A bioreactor, characterized in that: include: A culture tank and a magnetic stirring device as claimed in any one of claims 1 to 9; The culture tank comprises a tank body and a cover plate, wherein the cover plate is provided with a through hole, and the stirring shaft (2) of the magnetic stirring device extends into the tank body through the through hole, and the connecting piece (8) of the magnetic stirring device is fixedly connected to the cover plate via a cover plate connecting portion (83).

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