Centrifugal sedimentation device and bacteriophage display device with same

Abstract

The utility model provides a centrifugal sedimentation device and a bacteriophage display device with the centrifugal sedimentation device. The rotating motor is fixedly assembled on the centrifugal device bracket; the centrifugal rotor is fixedly connected to a rotating shaft of the rotating motor, a plurality of bottle body fixing cups are arranged on the centrifugal rotor, and the bottle body fixing cups have at least two kinds of caliber sizes so that at least two kinds of centrifugal bottles of different specifications can be placed and positioned. The centrifugal sedimentation device can be suitable for centrifugal sedimentation operation of at least two centrifugal bottles with different specifications at the same time, different centrifugal sedimentation devices do not need to be configured for the centrifugal bottles with different specifications, the centrifugal sedimentation device is high in integration degree, the requirement for the installation space can be effectively lowered, and the equipment manufacturing and using cost is lowered.

Description

Technical Field

[0001] The utility model belongs to the technical field of phage display equipment design, and in particular relates to a centrifugal sedimentation device and a phage display device having the same. Background Art

[0002] Phage is a type of tiny life form that infects and replicates inside bacteria. Using synthetic biology tools, the genetic information of phage can be reprogrammed, making phage or infected bacteria an important tool for drug screening, protein optimization, and biomarker screening.

[0003] Phage display technology is one of the two major development platforms for all approved antibody drugs in the world. Due to its mature research system, low cost, and simple operation, phage display technology has attracted widespread attention from researchers and has extremely important commercial value.

[0004] In the field of antibody preparation, phage display technology provides a simple and fast genetic engineering antibody preparation route for fully human recombinant antibodies: a complete set of antibody heavy chain variable region (VH) and light chain variable region (VL) genes are amplified from human immune cells using PCR technology and expressed on the phage surface in the form of fusion proteins.

[0005] Various types of antibodies can be screened through phage display technology. Compared with monoclonal antibodies produced by immunizing animals, monoclonal antibodies produced using this technology have the advantages of higher efficiency and faster production.

[0006] The current phage display technology is implemented by a combination of manual operations of equipment such as a full-temperature vibration incubator, a centrifuge, and a cloning and panning device. The aforementioned equipment is a single-function and independent device, and requires operator participation, which leads to the risk of cross-contamination during the culture and panning process. The manual participation will also lead to manual selection deviations, reducing the stability of the process and results. In addition, the independent application of single-function modules and the low degree of equipment integration lead to time-consuming and high-cost screening and display of phages. It should be noted that the centrifugal sedimentation device in the prior art is an independent application device, and the centrifugal sedimentation device is used to position and match with a centrifuge bottle of a single specification. Therefore, it cannot meet the multifunctional requirements of centrifugal sedimentation of sample liquids in centrifuge bottles of different specifications in an automated phage device. Based on the above-mentioned deficiencies in the prior art, the utility model proposes a centrifugal sedimentation device used in the phage display device. Utility Model Content

[0007] Therefore, the technical problem to be solved by the present invention is to provide a centrifugal sedimentation device and a phage display device having the same, so as to solve the technical problem that the centrifugal sedimentation device in the prior art cannot meet the multifunctional requirements of centrifugal sedimentation of sample liquids in centrifuge bottles of different specifications in an automated phage device.

[0008] In order to solve the above problems, the utility model provides a centrifugal sedimentation device, comprising:

[0009] Centrifugal device support;

[0010] A rotating motor, fixedly assembled on the centrifugal device bracket;

[0011] The centrifugal rotor is fixedly connected to the rotating shaft of the rotating motor and is provided with a plurality of bottle fixing cups. The caliber sizes of each of the bottle fixing cups are at least two, so as to place and position centrifugal bottles of at least two different specifications.

[0012] In some embodiments, the bottle fixing cups are spaced apart around the rotating shaft of the rotating motor and the central axes of the cup bodies of the bottle fixing cups are on the same circle.

[0013] In some embodiments, the centrifugal rotor has a plurality of open grooves extending radially and penetrating the outer peripheral wall, each of the bottle body fixing cups has a rotating shaft on the outer wall of the cup mouth, and the bottle body fixing cup is rotatably mounted on the two side groove walls of the open groove through the rotating shaft.

[0014] In some embodiments, the bottom of the centrifuge bottle is a conical structure, and the shape of the bottle body fixing cup is the same as the outer shape of the centrifuge bottle; and / or,

[0015] A rotating shaft pressing block is detachably connected to the top surface of each of the open grooves, and each of the rotating shafts is located between the rotating shaft pressing block and the top surface of the centrifugal rotor.

[0016] In some embodiments, the centrifuge support includes a support plate body, and a plurality of Peltier assemblies for refrigeration are provided on the support plate body.

[0017] In some embodiments, a refrigeration sleeve is provided on the top side of the support plate body, the refrigeration sleeve is arranged around the outer circumference of the centrifugal rotor, the refrigeration sleeve is made of heat-conducting material and the bottom end surface is connected to the support plate body for heat exchange; and / or,

[0018] The cold end of the Peltier assembly is connected to the bracket plate body by heat exchange, and the hot end of the Peltier assembly is wrapped with a water cooling box, which is cyclically connected to the water cooling module.

[0019] In some embodiments, a protective sleeve is further included, which is disposed around the refrigeration sleeve and is located radially outside the refrigeration sleeve.

[0020] In some embodiments, a needle washing structure is further provided on the bracket plate body, and the needle washing structure includes a needle washing pool and a waste liquid buffer cavity. The needle washing pool is a blind hole with an opening facing upward. When the pipette needle is controlled to be inserted into the blind hole during the needle washing process, the waste liquid overflowing from the opening can be collected by the waste liquid buffer cavity; and / or, a plurality of support columns are connected to the bottom side surface of the bracket plate body, and a shock-absorbing column is provided between each of the support columns and the bracket plate body.

[0021] In some embodiments, the needle washing structure also includes a liquid passage cavity, which is arranged at intervals around the needle washing pool, and a cache shell is provided in the lower area of ​​the liquid passage cavity, the cache shell is connected with the liquid passage cavity and the waste liquid cache cavity is formed therebetween, a waste liquid pipe joint is provided at the bottom of the cache shell, and the waste liquid pipe joint is controllably connected with the waste liquid pool; and / or the central axis of the needle washing pool and the central axis of the cup body of each of the bottle body fixing cups are on the same circle.

[0022] The utility model also provides a phage display device, comprising the above-mentioned centrifugal sedimentation device.

[0023] The utility model provides a centrifugal sedimentation device and a phage display device having the same. Since bottle fixing cups with at least two caliber sizes are arranged on the same centrifugal rotor, the centrifugal sedimentation device of the utility model can be simultaneously applicable to the centrifugal sedimentation operations of at least two centrifuge bottles of different specifications, and there is no need to configure different centrifugal sedimentation devices for centrifuge bottles of different specifications. The centrifugal sedimentation device has a high degree of integration, can effectively reduce the demand for installation space, and reduce the equipment manufacturing and use costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 A schematic diagram of the top view of the centrifugal sedimentation device according to an embodiment of the utility model;

[0025] Figure 2 for Figure 1 Cross-sectional view of JJ;

[0026] Figure 3 for Figure 1 Cross-section of the middle LL;

[0027] Figure 4 for Figure 1 A bottom view of the centrifugal sedimentation device in FIG.

[0028] Figure 5 for Figure 1 Cross-sectional view of the needle washing pool structure.

[0029] The reference numerals are:

[0030] 31. Centrifugal device bracket; 311. Bracket plate; 312. Peltier assembly; 3121. Water cooling box; 313. Refrigeration sleeve; 314. Support column; 315. Shock-absorbing column; 32. Rotating motor; 33. Centrifugal rotor; 331. Bottle fixing cup; 3311. Rotating shaft; 3312. Rotating shaft pressing block; 34. Protective sleeve; 35. Needle washing structure; 351. Needle washing pool; 352. Waste liquid buffer chamber; 353. Buffer shell; 3531. Waste liquid pipe joint; 354. Liquid passage chamber; 300. Centrifugal bottle. DETAILED DESCRIPTION

[0031] See also Figures 1 to 5 As shown, according to an embodiment of the utility model, a centrifugal sedimentation device is provided, comprising: a centrifugal device bracket 31; a rotating motor 32, fixedly assembled on the centrifugal device bracket 31, specifically, the fixed shell of the rotating motor 32 is assembled on the centrifugal device bracket 31 through a corresponding motor support frame; a centrifugal rotor 33, fixedly connected to the rotating shaft of the rotating motor 32, so that when the rotating motor 32 is running, it can follow the rotating shaft of the rotating motor 32 to rotate at a high speed, thereby generating centrifugal force, and having a plurality of bottle body fixing cups 331 thereon, each of the bottle body fixing cups 331 has at least two caliber sizes, so as to place and position at least two centrifuge bottles 300 of different specifications, specifically refer to Figure 1 As shown in the figure, there are four bottle body fixing cups 331 with larger diameters and two bottle body fixing cups 331 with smaller diameters. In a specific embodiment, the four bottle body fixing cups 331 with larger diameters are respectively used to load 500ml centrifuge bottles, while the bottle body fixing cups 331 with smaller diameters are respectively used to load 5ml centrifuge bottles.

[0032] In this technical solution, since at least two bottle fixing cups 331 with different caliber sizes are provided on the same centrifugal rotor 33, the centrifugal sedimentation device of the utility model can be simultaneously applicable to the centrifugal sedimentation operation of at least two centrifuge bottles 300 with different specifications, and there is no need to configure different centrifugal sedimentation devices for centrifuge bottles with different specifications. The centrifugal sedimentation device has a high degree of integration, which can effectively reduce the demand for installation space and reduce the equipment manufacturing and use costs.

[0033] In some embodiments, each of the bottle body fixing cups 331 is arranged at intervals around the rotating shaft of the rotating motor 32 and the central axis of the cup body of each bottle body fixing cup 331 is on the same circle. Such a design can achieve corresponding adjustment of the position of the centrifuge bottle 300 fixedly arranged in each of the bottle body fixing cups 331 and the same pipetting needle by controlling the rotation angle of the rotating motor 32, and the position of the pipetting needle can be fixed, thereby eliminating the need to separately set a corresponding position adjustment structure for the pipetting needle, simplifying the design difficulty and reducing the manufacturing cost.

[0034] In some embodiments, see Figure 1 As shown, the centrifugal rotor 33 has a plurality of open grooves (not marked in the figure) extending along its radial direction and penetrating to the outer peripheral wall. The outer wall of the cup mouth of each bottle body fixing cup 331 is provided with a rotating shaft 3311. The bottle body fixing cup 331 is rotatably mounted on the groove walls on both sides of the open groove through the rotating shaft 3311. In this way, when the rotating motor 32 rotates, under the action of centrifugal force, each bottle body fixing cup 331 will be turned outward from a vertical state to a horizontal state, and the centrifugal force acts on the sample liquid in the centrifuge bottle 300 to form the centrifugal sedimentation of the internal substances, and the centrifugal sedimentation efficiency is higher.

[0035] In a preferred embodiment, the bottom of the centrifuge bottle 300 is a conical structure, and the shape of the bottle body fixing cup 331 is the same as the external shape of the centrifuge bottle 300. The centrifuge bottle 300 with a conical bottom structure can effectively reduce the dead volume in the bottle and ensure full utilization of the sample liquid. At the same time, the shape of the bottle body fixing cup 331 is the same as the external shape of the centrifuge bottle 300, which can ensure that the bottle body fixing cup 331 effectively supports the centrifuge bottle 300 and prevents the centrifuge bottle 300 from breaking under excessive centrifugal force.

[0036] In order to prevent the accidental separation of each bottle fixing cup 331 and the centrifugal rotor 33 during the centrifugal operation, in a preferred embodiment, a shaft pressing block 3312 is detachably connected to the top surface of each opening groove, and each shaft 3311 is located between the shaft pressing block 3312 and the top surface of the centrifugal rotor 33.

[0037] See also Figure 4 As shown, in some embodiments, the centrifuge support 31 includes a support plate 311, on which a plurality of Peltier assemblies 312 for cooling are disposed, and the plurality of Peltier assemblies 312 are evenly spaced around the rotating shaft of the rotating motor 32 to ensure uniform temperature adjustment.

[0038] See Figure 2 and Figure 3As shown, in some embodiments, a refrigeration sleeve 313 is provided on the top side surface of the support plate 311, and the refrigeration sleeve 313 is arranged around the outer periphery of the centrifugal rotor 33. The refrigeration sleeve 313 is made of a heat-conducting material (such as aluminum or copper) and the bottom end surface is heat-exchanged with the support plate 311. The refrigeration sleeve 313 can surround the centrifugal rotor 33 on the one hand, and can protect the bottle fixing cups 331 and the centrifugal bottles 300 that are rotating at high speed in a centrifugal state from detachment or rupture. More importantly, it can more efficiently conduct the cold generated by the Peltier components 312 at the bottom to the upper space, thereby ensuring the refrigeration efficiency and refrigeration effect.

[0039] The cold end of the Peltier component 312 is connected to the bracket plate body 311 in a heat exchange manner, and the hot end of the Peltier component 312 is wrapped with a water cooling box 3121, and the water cooling box 3121 is cyclically connected to a water cooling heat dissipation module (not shown in the figure). Specifically, the aforementioned water cooling box 3121 is fixed to the bottom side of the bracket plate body 311 by a corresponding hoop (not marked in the figure), and the aforementioned water cooling heat dissipation module can be a water cooling row, which is equipped with a corresponding fan to be able to efficiently dissipate the cooling water flowing into it.

[0040] In some embodiments, the centrifugal sedimentation device further includes a protective sleeve 34, which is arranged around the refrigeration sleeve 313 and is located radially outside the refrigeration sleeve 313. The protective sleeve 34 surrounding the outside of the refrigeration sleeve 313 can further improve the operating safety of the centrifugal sedimentation device.

[0041] See also Figure 1 and Figure 5 As shown, in some embodiments, a needle washing structure 35 is further provided on the bracket plate body 311, and the needle washing structure 35 includes a needle washing pool 351 and a waste liquid buffer chamber 352. The needle washing pool 351 is a blind hole with an opening facing upward. When the pipette needle is controlled to be inserted into the blind hole during the needle washing process, the waste liquid overflowing from the opening can be collected by the waste liquid buffer chamber 352. In this technical solution, the needle washing pool 351 is a blind hole. When the pipette needle is inserted into the blind hole and the cleaning liquid is sprayed, the sprayed cleaning liquid will collide with the bottom wall of the blind hole and overflow upward along the vertical wall of the blind hole. The overflow process can clean the outer wall of the pipette needle, and the overflowed cleaning liquid is stored and collected in the waste liquid buffer chamber 352.

[0042] In some embodiments, the needle washing structure 35 also includes a liquid passage chamber 354, which is arranged at intervals around the needle washing pool 351, and a cache shell 353 is provided in the lower area of ​​the liquid passage chamber 354. The cache shell 353 is connected to the liquid passage chamber 354 and forms the waste liquid cache chamber 352 therebetween. A waste liquid pipe joint 3531 is provided at the bottom of the cache shell 353, and the waste liquid pipe joint 3531 is controllably connected to the waste liquid pool (not shown in the figure). In this technical solution, the cache shell 353 can be threadedly connected to form an assembly, thereby forming the aforementioned waste liquid cache chamber 352. Such a design can reduce the structural design.

[0043] The central axis of the needle washing pool 351 and the central axis of the cup body of each of the bottle body fixing cups 331 are on the same circle to achieve the purpose of in-situ cleaning of the aforementioned liquid-absorbing needles.

[0044] A plurality of support columns 314 are connected to the bottom side of the support plate body 311, and a shock-absorbing column 315 is provided between each of the support columns 314 and the support plate body 311. The shock-absorbing column 315 can specifically be a shock-absorbing rubber column, which can absorb the vibration generated during the operation of the centrifugal rotor 33 to a certain extent and reduce the noise level of the device.

[0045] According to an embodiment of the utility model, a phage display device is also provided, comprising the above-mentioned centrifugal sedimentation device.

[0046] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0047] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred implementations of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A centrifugal sedimentation device, characterized in that: include: Centrifugal device support (31); A rotating motor (32) is fixedly assembled on the centrifugal device support (31); The centrifugal rotor (33) is fixedly connected to the rotating shaft of the rotating motor (32) and has a plurality of bottle fixing cups (331) thereon. The caliber sizes of each of the bottle fixing cups (331) are at least two, so as to place and position centrifugal bottles (300) of at least two different specifications.

2. The centrifugal sedimentation device according to claim 1, characterized in that: The bottle body fixing cups (331) are arranged at intervals around the rotating shaft of the rotating motor (32), and the central axes of the cup bodies of the bottle body fixing cups (331) are located on the same circle.

3. The centrifugal sedimentation device according to claim 2, characterized in that: The centrifugal rotor (33) has a plurality of open grooves extending in its radial direction and penetrating to the outer peripheral wall. The outer wall of the cup opening of each bottle body fixing cup (331) is provided with a rotating shaft (3311). The bottle body fixing cup (331) is rotatably mounted on the groove walls on both sides of the open groove via the rotating shaft (3311).

4. The centrifugal sedimentation device according to claim 3, characterized in that: The bottom of the centrifuge bottle (300) is a conical structure, and the shape of the bottle body fixing cup (331) is the same as the external shape of the centrifuge bottle (300); and / or, A rotating shaft pressing block (3312) is detachably connected to the top surface of each of the open grooves, and each of the rotating shafts (3311) is located between the rotating shaft pressing block (3312) and the top surface of the centrifugal rotor (33).

5. The centrifugal sedimentation device according to claim 1, characterized in that: The centrifuge support (31) comprises a support plate body (311), and a plurality of Peltier assemblies (312) for cooling are arranged on the support plate body (311).

6. The centrifugal sedimentation device according to claim 5, characterized in that: A refrigeration sleeve (313) is provided on the top side of the support plate body (311), the refrigeration sleeve (313) is arranged around the outer circumference of the centrifugal rotor (33), the refrigeration sleeve (313) is made of heat-conducting material, and the bottom end surface is connected to the support plate body (311) by heat exchange; and / or, The cold end of the Peltier component (312) is connected to the support plate body (311) by heat exchange, and the hot end of the Peltier component (312) is wrapped with a water cooling box (3121), and the water cooling box (3121) is cyclically connected to a water cooling and heat dissipation module.

7. The centrifugal sedimentation device according to claim 6, characterized in that: It also includes a protective sleeve (34), which is arranged around the refrigeration sleeve (313) and is located radially outside the refrigeration sleeve (313).

8. The centrifugal sedimentation device according to claim 5, characterized in that: The support plate body (311) is also provided with a needle washing structure (35), and the needle washing structure (35) includes a needle washing pool (351) and a waste liquid buffer chamber (352), and the needle washing pool (351) is a blind hole with an opening facing upwards, and when the aspiration needle is controlled to be inserted into the blind hole during the needle washing process, the waste liquid overflowing from the opening can be collected by the waste liquid buffer chamber (352); and / or, A plurality of support columns (314) are connected to the bottom side of the support plate body (311), and a shock absorbing column (315) is provided between each of the support columns (314) and the support plate body (311).

9. The centrifugal sedimentation device according to claim 8, characterized in that: The needle washing structure (35) further comprises a liquid passage cavity (354), wherein the liquid passage cavity (354) is arranged at intervals around the needle washing pool (351), and a cache shell (353) is provided in the lower area of ​​the liquid passage cavity (354), wherein the cache shell (353) is connected with the liquid passage cavity (354) and the waste liquid cache cavity (352) is formed therebetween, and a waste liquid pipe joint (3531) is provided at the bottom of the cache shell (353), and the waste liquid pipe joint (3531) is controllably connected with the waste liquid pool; and / or, The central axis of the needle washing pool (351) and the central axis of the cup body of each bottle body fixing cup (331) are located on the same circle.

10. A phage display device, characterized in that: A centrifugal sedimentation device comprising any one of claims 1 to 9.

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