Organ-like culture device
By designing telescopic clamping components in the organoid culture device, the problem of limited size of culture vessels in the prior art is solved, and stable clamping and rotation of test tubes of different sizes is achieved, which improves the practicality of the device and cell culture efficiency. .
Patent Information
- Application Number
- CN202421131710.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-23
- Publication Date
- 2025-02-25
- Estimated Expiration
- 2034-05-23
AI Technical Summary
When existing organoid cell culture devices are clamped and fixed against culture instruments of different sizes, they cannot be stable clamped, resulting in a very limited size of the culture vessel.
An organoid culture device is designed, using a telescopic clamping assembly, including an electric slider, an electric push rod and a clamping block, which slides in the circular slide through an electric slider. The electric push rod expands and contracts according to the distance of the test tube to make the clamping. The block is able to hold the test tubes securely, ensuring that the device remains stable during rotation and adapts to test tubes of different sizes.
The stable clamping and rotation of test tubes of different sizes is achieved, which improves the practicality of the device and its efficiency in cell culture.
Smart Images
Figure CN222524566U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to an organoid culture device. Background Art
[0002] Organoids are "micro-organs" cultivated by stem cells in an in vitro environment. They contain a variety of tissue-specific cell types and self-organize into three-dimensional tissue structures. They can partially simulate the structure and function of native organs. Currently, organoids are widely used in drug toxicity testing, efficacy evaluation and screening, and establishment of disease models to study genetic diseases, infectious diseases, cancer and other fields.
[0003] In the existing organoid cell culture process, multiple culture through-holes are usually used for culture. However, when the culture vessel is clamped and fixed on culture vessels of different sizes, it is impossible to clamp stably, which makes the size of the culture vessel very limited. Utility Model Content
[0004] The purpose of the utility model is to provide an organoid culture device to solve the problem raised in the above-mentioned background technology that, during the culture process, a plurality of culture through holes are usually used for culture, but the culture vessel cannot be stably clamped when clamping and fixing culture vessels of different sizes, so that the size of the culture vessel is very limited. To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an organoid culture device, comprising a device body, a culture dish is sleeved inside the device body, a test tube rack is movably connected inside the culture dish, a telescopic clamping assembly is arranged on the surface of the test tube rack, the telescopic clamping assembly comprises a circular slide groove, the circular slide groove is arranged on the surface of the test tube rack, an electric slider is movably connected inside the circular slide groove, a hole is arranged on one side of the electric slider, an electric push rod is movably connected inside the hole, a clamping block is sleeved inside the electric push rod, and by installing the telescopic clamping assembly, the electric slider can slide inside the circular slide groove to both sides of the test tube, the electric push rod is telescoped according to the distance between the test tubes, so that the clamping block can fix and clamp the test tube in the culture dish, so that the device can remain stable during rotation, and the device can also fix and clamp test tubes of different sizes, thereby improving the practicality of the device.
[0005] Further preferably, a test tube hole is provided on the surface of the test tube rack, a rotating disk is fixedly connected to the bottom of the culture dish, a limiting hole is provided on the surface of the rotating disk, a sponge block is fixedly connected to the inside of the limiting hole, and a rotating assembly is provided at the bottom of the rotating disk. By installing the sponge block, the test tube can be placed in the limiting hole on the surface of the rotating disk, so that the test tube can be fixed, and at the same time, the buffering force of the test tube during the rotation process is increased, thereby improving the practicability of the device.
[0006] Further preferably, the rotating assembly includes a mounting plate, the mounting plate is fixedly connected to the bottom wall inside the device body, the upper surface of the mounting plate is fixedly connected to a rotating motor, the bottom of the rotating disk is fixedly connected to a connecting plate, the driving end of the rotating motor is fixedly connected to a rotating rod, and the driving end of the rotating motor is fixedly connected to the connecting plate via the rotating rod. By installing the rotating assembly, the rotating motor can be started to drive the rotating rod to rotate, thereby enabling the test tube on the turntable to rotate, thereby accelerating the mixing of the nutrient solution inside the test tube, and allowing the bubbles generated inside the test tube to dissipate during rotation, thereby accelerating the cell culture inside the test tube, thereby improving the practicality of the device in cell culture.
[0007] Further preferably, an observation window is fixedly provided on the front of the device body, and transparent glass is fixedly connected to the inside of the observation window. A control panel is fixedly connected to the other side of the device body. By installing the transparent glass, researchers can observe and record the cell culture inside the test tube through the transparent glass, and it can also help to observe the growth of cells without interfering with them.
[0008] Further preferably, a display screen is fixedly connected to the front of the control panel, an adjustment button is fixedly connected to one side of the display screen, and a control switch is fixedly connected below the adjustment button. By installing the control panel, the motor speed in the rotating assembly of the device can be set and adjusted through the adjustment button, and the culture status of the culture dish inside the device can be displayed on the display screen, which is convenient for researchers to record. Finally, the device can be controlled to operate through the control switch, thereby improving the convenience of operating the device.
[0009] Further preferably, a top cover is movably connected to the top of the device body, a fixed block is fixedly connected to the top of the top cover, a handle is fixedly connected to the upper surface of the fixed block, and the device can be carried and moved via the handle, thereby improving the practicability of the device.
[0010] Compared with the prior art, the utility model has the following beneficial effects:
[0011] In the utility model, by installing a telescopic clamping assembly, the electric slider can slide inside the circular slide groove and slide to both sides of the test tube. The electric push rod is extended and retracted according to the distance between the test tubes, so that the clamping block can fix and clamp the test tube in the culture dish, so that the device can remain stable during the rotation process. At the same time, the device can also fix and clamp test tubes of different sizes, thereby improving the practicality of the device.
[0012] In the utility model, by installing a rotating component, the rotating motor can be started, and the rotating rod can be driven to rotate by the motor, so that the test tube on the turntable can be rotated, thereby accelerating the mixing of the nutrient solution inside the test tube, allowing the bubbles generated inside the test tube to dissipate during the rotation, thereby accelerating the cell culture inside the test tube and improving the practicability of the device in cell culture. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 The three-dimensional structure of the utility model is shown in FIG. Figure 1 ;
[0014] Figure 2 For this utility model Figure 1 A in the middle is an enlarged schematic diagram of the three-dimensional structure;
[0015] Figure 3 The three-dimensional structure of the utility model is shown in FIG. Figure 2 ;
[0016] Figure 4 This is a schematic diagram of the cross-sectional structure of the utility model;
[0017] Figure 5 For this utility model Figure 4 Enlarged cross-sectional structural diagram at point B in the middle.
[0018] In the figure: 1. device body; 2. culture dish; 3. test tube rack; 4. telescopic clamping assembly; 5. test tube hole; 6. rotating disk; 7. limit hole; 8. sponge block; 9. rotating assembly; 10. observation window; 11. transparent glass; 12. control panel; 13. top cover; 14. fixing block; 15. handle; 401. circular slide groove; 402. electric slider; 403. hole; 404. electric push rod; 405. clamping block; 901. mounting plate; 902. rotating motor; 903. connecting plate; 904. rotating rod; 1201. display screen; 1202. adjustment button; 1203. control switch. DETAILED DESCRIPTION
[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technical personnel in this field without creative work are within the scope of protection of the utility model.
[0020] See also Figure 1 - Figure 5The utility model provides a technical solution: an organoid culture device, including a device body 1, a culture dish 2 is sleeved inside the device body 1, a test tube rack 3 is movably connected inside the culture dish 2, a telescopic clamping component 4 is arranged on the surface of the test tube rack 3, the telescopic clamping component 4 includes a circular slide groove 401, the circular slide groove 401 is opened on the surface of the test tube rack 3, an electric slider 402 is movably connected inside the circular slide groove 401, a hole 403 is opened on one side of the electric slider 402, an electric push rod 404 is movably connected inside the hole 403, a clamping block 405 is sleeved inside the electric push rod 404, and the telescopic clamping component 4 is installed. When in use, the electric slider 402 slides inside the circular slide groove 401 to slide to both sides of the test tube, and the electric push rod 404 is retracted according to the distance between the test tubes, so that the clamping block 405 can fix and clamp the test tube in the culture dish 2.
[0021] In this embodiment, Figure 1 , Figure 2 and Figure 4 As shown, a test tube hole 5 is provided on the surface of the test tube rack 3, a rotating disk 6 is fixedly connected to the bottom of the culture dish 2, a limiting hole 7 is provided on the surface of the rotating disk 6, a sponge block 8 is fixedly connected to the inside of the limiting hole 7, and a rotating component 9 is provided at the bottom of the rotating disk 6. When in use, the test tube is fixed inside the culture dish 2 through the test tube hole 5, and at the same time, the bottom of the test tube is placed in the limiting hole 7 on the surface of the rotating disk 6 through the sponge block 8, so that the test tube is fixedly placed.
[0022] In this embodiment, Figure 4 and Figure 5 As shown, the rotating component 9 includes a mounting plate 901, which is fixedly connected to the bottom wall inside the device body 1, and a rotating motor 902 is fixedly connected to the upper surface of the mounting plate 901. A connecting plate 903 is fixedly connected to the bottom of the rotating disk 6, and a driving end of the rotating motor 902 is fixedly connected to a rotating rod 904. The driving end of the rotating motor 902 is fixedly connected to the connecting plate 903 through the rotating rod 904. By installing the rotating component 9, when in use, the rotating motor 902 is started, and the rotating rod 904 is driven to rotate by the rotating motor 902, so that the test tube on the rotating disk 6 can be rotated, thereby accelerating the mixing of the nutrient solution inside the test tube, and allowing the bubbles generated inside the test tube to dissipate during rotation.
[0023] In this embodiment, Figure 1 As shown, an observation window 10 is fixedly provided on the front of the device body 1, and a transparent glass 11 is fixedly connected to the inside of the observation window 10. A control panel 12 is fixedly connected to the other side of the device body 1. When in use, the researcher observes and records the cell culture growth inside the test tube through the transparent glass 11 inside the observation window 10.
[0024] In this embodiment, Figure 1and Figure 3 As shown, a display screen 1201 is fixedly connected to the front of the control panel 12, an adjustment button 1202 is fixedly connected to one side of the display screen 1201, and a control switch 1203 is fixedly connected below the adjustment button 1202. By installing the control panel 12, when in use, the speed of the rotating motor 902 in the rotating component 9 of the device is set and adjusted by adjusting the adjustment button 1202 on the control panel 12, and at the same time, the culture status of the culture dish 2 inside the device is displayed on the display screen 1201, so that researchers can record it, and finally the device body 1 is started by the control switch 1203.
[0025] In this embodiment, Figure 3 and Figure 4 As shown, the top of the device body 1 is movably connected to a top cover 13, the top of the top cover 13 is fixedly connected to a fixing block 14, and the upper surface of the fixing block 14 is fixedly connected to a handle 15. When in use, the device body 1 is carried and moved by the handle 15.
[0026] The use method and advantages of the utility model: When the organoid culture device is used, the working process is as follows:
[0027] like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, when in use, the selected appropriate cell line is first placed in a test tube containing an organoid culture solution for culture, and the test tube is placed in the internal test tube rack 3 of the culture dish 2. The bottom of the test tube is placed in the limiting hole 7 on the surface of the rotating disk 6 through the sponge block 8. The upper part of the test tube slides inside the circular slide groove 401 through the electric slider 402, and slides to both sides of the test tube. The electric push rod 404 is extended and retracted according to the distance between the test tubes, so that the clamping block 405 can fix and clamp the test tube in the culture dish 2. At the same time, nutrient solution needs to be added to it during the culture process, and the adjustment button 120 on the control panel 12 is adjusted. The rotation speed of the rotating motor 902 in the rotating assembly 9 of the device is set and adjusted, and the culture status of the culture dish 2 inside the device is displayed on the display screen 1201, so that the researchers can record it. Finally, the rotating motor 902 is started by the control switch 1203, and the rotating rod 904 is driven to rotate by the rotating motor 902, so that the test tube on the rotating disk 6 can be rotated, the nutrient solution inside the test tube is mixed faster, and the bubbles generated inside the test tube can be dissipated during the rotation. At the same time, the cell culture growth inside the test tube can be observed and recorded through the transparent glass 11 inside the observation window 10.
[0028] The above shows and describes the basic principle, main features and advantages of the utility model. Technical staff in this industry should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model will have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.
Claims
1. An organoid culture device, comprising a device body (1), characterized in that: A culture dish (2) is sleeved inside the device body (1), and a test tube rack (3) is movably connected inside the culture dish (2). A telescopic clamping assembly (4) is arranged on the surface of the test tube rack (3). The telescopic clamping assembly (4) comprises a circular slide groove (401), and the circular slide groove (401) is provided on the surface of the test tube rack (3). An electric slider (402) is movably connected inside the circular slide groove (401), and a hole (403) is provided on one side of the electric slider (402). An electric push rod (404) is movably connected inside the hole (403), and a clamping block (405) is sleeved inside the electric push rod (404).
2. The organoid culture device according to claim 1, characterized in that: The surface of the test tube rack (3) is provided with a test tube hole (5); the bottom of the culture dish (2) is fixedly connected to a rotating disk (6); the surface of the rotating disk (6) is provided with a limiting hole (7); the interior of the limiting hole (7) is fixedly connected to a sponge block (8); and the bottom of the rotating disk (6) is provided with a rotating assembly (9).
3. The organoid culture device according to claim 2, characterized in that: The rotating assembly (9) comprises a mounting plate (901), wherein the mounting plate (901) is fixedly connected to the bottom wall inside the device body (1), a rotating motor (902) is fixedly connected to the upper surface of the mounting plate (901), a connecting plate (903) is fixedly connected to the bottom of the rotating disk (6), a driving end of the rotating motor (902) is fixedly connected to a rotating rod (904), and the driving end of the rotating motor (902) is fixedly connected to the connecting plate (903) via the rotating rod (904).
4. The organoid culture device according to claim 1, characterized in that: An observation window (10) is fixedly provided on the front of the device body (1), a transparent glass (11) is fixedly connected to the inside of the observation window (10), and a control panel (12) is fixedly connected to the other side of the device body (1).
5. The organoid culture device according to claim 4, characterized in that: A display screen (1201) is fixedly connected to the front of the control panel (12), an adjustment button (1202) is fixedly connected to one side of the display screen (1201), and a control switch (1203) is fixedly connected below the adjustment button (1202).
6. The organoid culture device according to claim 1, characterized in that: The top of the device body (1) is movably connected to a top cover (13), the top of the top cover (13) is fixedly connected to a fixing block (14), and the upper surface of the fixing block (14) is fixedly connected to a handle (15).