The invention relates to the technical field of electrostatic spinning, in particular to an air flow assisting handheld portable electrostatic spinning device and application thereof. The electrostatic spinning device comprises an insulating shell, a large-capacity secondary battery, a charging slot, a high-voltage wire, a high-voltage static electricity generating unit, a high-voltage static electricity generating switch, an air flow guide pipe connector, an air flow guide pipe, an injector fixing support, a disposable injector, a needle fixing support, a stainless steel needle and an air flow oriented cover. The large-capacity secondary battery is adopted as the power supply, and high-voltage static electricity is generated through the high-voltage static electricity generating unit; theelectrostatic spinning device is externally connected with an air flow generator through the air flow guide pipe connector, the stainless steel needle and the air flow guide pipe are fixedly locatedon the same axis, and electrostatic spinning is conducted with the aid of air flow. The electrostatic spinning device is high in integration degree, convenient to carry and capable of being held by one hand, and the orientation, uniformity and high-efficiency deposition of electrostatic spinning fibers are realized with the aid of air flow.