Mobile phase bottle for liquid chromatography and high performance liquid chromatography system

Abstract

The utility model relates to a mobile phase bottle for liquid chromatography. The mobile phase bottle comprises a liquid phase bottle body, a liquid adding funnel, a liquid adding opening bottle cap, a liquid inlet pipe, a mobile phase filter and a liquid inlet bottle cap, one end of the liquid adding funnel extends into the liquid phase bottle body, and the other end of the liquid adding funnel is connected with the liquid adding opening bottle cap; the input end of the liquid inlet pipe extends into the bottom of the liquid phase bottle body and is connected with the end of the mobile phase filter. The utility model also relates to a high performance liquid chromatography system using the mobile phase bottle for liquid chromatography. The mobile phase can be added at any time in the detection process of a high performance liquid chromatography system, so that the generation of bubbles can be reduced, the volume of the mobile phase always meets the dosage required by detection, the error rate of an instrument caused by insufficient mobile phase is reduced, and the detection success rate is improved.

Description

Technical Field

[0001] The utility model relates to the field of liquid replenishing devices for detection instruments, in particular to a mobile phase bottle for liquid chromatography, and also to a high performance liquid chromatography system with the mobile phase bottle for liquid chromatography. Background Art

[0002] High Performance Liquid Chromatography (HPLC) is also known as "high pressure liquid chromatography", "high speed liquid chromatography", "high resolution liquid chromatography", "modern column chromatography", etc. High performance liquid chromatography is an important branch of chromatography. It uses liquid as the mobile phase and a high pressure infusion system to pump single solvents with different polarities or mixed solvents with different proportions, buffers and other mobile phases into a chromatographic column with a stationary phase. After the components in the column are separated, they enter the detector for detection, thereby realizing the analysis of the sample. This method has become an important separation and analysis technology application in the fields of chemistry, medicine, industry, agronomy, commercial inspection and legal inspection.

[0003] In the prior art, the mobile phase for liquid chromatography is mostly stored in a glass solvent bottle. The mobile phase is connected to the high-performance liquid chromatograph through a hose with a suction filter head, and is extracted from the solvent bottle by a high-pressure infusion pump for use in the test. Most of the existing liquid chromatographs adopt a multi-channel structure, and each channel uses an independent solvent bottle. Due to the limited capacity of the solvent bottle, when the mobile phase flow rate required by the experiment is large or the detection duration is long, it is necessary to add the mobile phase to the solvent bottle in time. In addition, during the detection process, there may be instrument errors that require re-measurement, or insufficient mobile phase configuration, etc., which makes the required volume of the mobile phase exceed the estimate and is exhausted in advance, and ultimately causes the pump of the liquid chromatograph to be unable to inject the mobile phase and suspend work. Utility Model Content

[0004] The utility model aims to provide a mobile phase bottle for liquid chromatography, which solves the problem in the prior art that the mobile phase storage volume is small and liquid cannot be replenished in time during the detection process.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] The first aspect of the utility model provides a mobile phase bottle for liquid chromatography, comprising a liquid phase bottle body, a liquid adding funnel, a liquid adding port bottle cover, a liquid inlet tube, a mobile phase filter and a liquid inlet bottle cover; one end of the liquid adding funnel extends into the liquid phase bottle body, and the other end of the liquid adding funnel is connected to the liquid adding port bottle cover; the input end of the liquid inlet tube extends into the bottom of the liquid phase bottle body and is terminated with the mobile phase filter.

[0007] Furthermore, the liquid adding funnel comprises an integrally formed funnel body and a funnel neck, the funnel body comprises a buffer liquid storage cavity, and a drainage bevel is arranged at the lower end of the funnel neck.

[0008] Furthermore, the mobile phase filter is selected from one or more of a stainless steel filter, a ceramic filter, a glass sand core filter and a PEEK online filter.

[0009] Furthermore, the liquid adding port bottle cap and the liquid inlet bottle cap are screw caps or plastic bottle caps.

[0010] Furthermore, the material of the liquid filling port bottle cap and the liquid inlet bottle cap is selected from one or more of PTFE, polypropylene and Teflon.

[0011] Furthermore, there is a certain gap between the contact surface of the liquid inlet pipe and the liquid inlet bottle cap for ventilation.

[0012] The second aspect of the utility model provides a high performance liquid chromatography system, comprising a mobile phase bottle for liquid chromatography, a pump, an automatic sampler, a chromatographic column, a detector and a data acquisition and processing system, wherein the output end of the liquid inlet tube in the mobile phase bottle for liquid chromatography is connected to the input end of the pump, the output end of the pump is connected to the input end of the automatic sampler, the output end of the automatic sampler is connected to the input end of the chromatographic column, the output end of the chromatographic column is connected to the input end of the detector, and the output end of the detector is connected to the data acquisition and processing system.

[0013] Due to the application of the above technical solution, the utility model has the following advantages compared with the prior art:

[0014] The utility model discloses a mobile phase bottle for liquid chromatography, in which a liquid adding port is provided on the side of a conventional liquid phase bottle, and a liquid adding funnel is connected to the liquid adding port and extends into the liquid phase bottle. With this arrangement, the amount of mobile phase can be added at any time according to the actual detection needs during the detection process, so that the volume of the mobile phase always meets the required amount for the detection and is maintained until the end of the measurement; at the same time, the liquid adding funnel extends below the liquid surface of the mobile phase, plays a liquid sealing role, and effectively reduces the generation of bubbles when the mobile phase is added in the middle. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Hereinafter, some specific embodiments of the present invention will be described in detail in an exemplary and non-limiting manner with reference to the accompanying drawings. The same reference numerals in the accompanying drawings indicate the same or similar components or parts. It should be understood by those skilled in the art that these drawings are not necessarily drawn to scale. In the accompanying drawings:

[0016] Figure 1 This is a device diagram of a mobile phase bottle for liquid chromatography provided by the utility model;

[0017] Figure 2This is a schematic diagram of the structure of a high performance liquid chromatography system provided by the utility model;

[0018] The reference numerals are described as follows:

[0019] 1. Liquid phase bottle body; 2. Liquid addition funnel; 3. Liquid addition bottle cap; 4. Liquid inlet tube; 5. Mobile phase filter; 6. Liquid inlet bottle cap; 7. Pump; 8. Automatic sampler; 9. Chromatographic column; 10. Detector; 11. Data acquisition and processing system. DETAILED DESCRIPTION

[0020] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See also Figure 1 As shown, the mobile phase bottle is composed of a liquid phase bottle body 1, a liquid adding funnel 2, a liquid adding port bottle cover 3, a liquid inlet tube 4, a mobile phase filter 5 and a liquid inlet bottle cover 6.

[0022] The liquid phase bottle body 1 is used to hold the mobile phase or organic solvent, and is generally made of high borosilicate glass. In special cases, a liquid phase bottle made of polyvinyl chloride is used to store the mobile phase. In addition, when storing the mobile phase, the color of the liquid phase bottle body 1 will change according to the characteristics of the mobile phase. For example, when storing the water phase, a brown liquid phase bottle is often used to avoid light to reduce the growth rate of bacteria.

[0023] In this embodiment, a liquid replenishment port is provided on the side of the liquid phase bottle body 1, and a liquid replenishment port bottle cap 3 is provided on the liquid replenishment port. A liquid adding funnel 2 extending into the liquid phase bottle body 1 is fixedly connected below the liquid adding port bottle cap 3. The liquid adding funnel 2 includes an integrally formed funnel body 21 and a funnel neck 22. The funnel body 21 includes a buffer liquid storage chamber 211. The buffer liquid storage chamber 211 uses a pear-shaped port, which can effectively buffer the impact of the original mobile phase in the liquid phase bottle body 1 during liquid replenishment to prevent the mobile phase from splashing and generating bubbles. A drainage bevel 221 is provided at the lower end of the funnel neck 22 to facilitate drainage of liquid.

[0024] A liquid inlet bottle cap 6 is provided on the liquid phase bottle body 1, and the liquid inlet bottle cap 6 is used in conjunction with the liquid phase bottle body 1. The liquid inlet tube 4 passes through the hole on the liquid inlet bottle cap 6 and is inserted into the liquid phase bottle body 1. There is a certain gap between the liquid inlet tube 4 and the hole on the liquid inlet bottle cap 6 for ventilation to prevent the negative pressure in the liquid phase bottle body 1 from being too low, resulting in the mobile phase being unable to be smoothly output to the pump body of the high performance liquid chromatography system.

[0025] Moreover, the liquid adding port bottle cap 3 and the liquid inlet bottle cap 6 are screw caps or plastic caps, which are beneficial to ensure the sealing of the liquid phase bottle; the material of the liquid adding port bottle cap 3 and the liquid inlet bottle cap 6 is generally selected from PTFE, polypropylene or Teflon.

[0026] The input end of the liquid inlet tube 4 extends into the bottom of the liquid phase bottle body 1 and is connected to the mobile phase filter 5; the mobile phase filter 5 is generally a stainless steel filter, a ceramic filter, a glass sand core filter or a PEEK online filter.

[0027] See also Figure 2 As shown, when the high performance liquid chromatography system in the utility model is working, the working process is as follows: the pump 7 provides power, and the mobile phase is extracted from the liquid inlet pipe 4 in the liquid phase bottle body 1 through the mobile phase filter 5 to reach the automatic sampler 8, and then the mobile phase sends the sample to be detected into the chromatographic column 9 for separation, and the separated components flow into the detector 10 in turn, and the detection data is collected by the data acquisition and processing system 11; if an error is encountered during the operation of the system and re-measurement is required, or the remaining volume of the mobile phase in the liquid phase bottle body 1 cannot meet the required volume of the sample for measurement, the liquid filling port bottle cap 3 can be opened, and the newly configured mobile phase can be added to the liquid phase bottle body 1 through the liquid addition funnel 2, which will not affect the continuous operation and content determination of the high performance liquid chromatography system.

[0028] The above embodiments are only for illustrating the technical concept and features of the utility model, and their purpose is to enable people familiar with this technology to understand the content of the utility model and implement it accordingly. They cannot be used to limit the protection scope of the utility model. All equivalent changes or modifications made according to the spirit of the utility model should be included in the protection scope of the utility model.

Claims

1. A mobile phase bottle for liquid chromatography, characterized in that: The liquid phase bottle comprises a liquid phase bottle body (1), a liquid adding funnel (2), a liquid adding port bottle cover (3), a liquid inlet tube (4), a mobile phase filter (5) and a liquid inlet bottle cover (6); one end of the liquid adding funnel (2) extends into the liquid phase bottle body (1), and the other end of the liquid adding funnel (2) is connected to the liquid adding port bottle cover (3); the input end of the liquid inlet tube (4) extends into the bottom of the liquid phase bottle body (1) and is connected to the mobile phase filter (5).

2. A mobile phase bottle for liquid chromatography according to claim 1, characterized in that, The liquid adding funnel (2) comprises an integrally formed funnel body (21) and a funnel neck (22); the funnel body (21) comprises a buffer liquid storage cavity (211); and a drainage bevel (221) is provided at the lower end of the funnel neck (22).

3. A mobile phase bottle for liquid chromatography according to claim 1, characterized in that, The mobile phase filter (5) is selected from a stainless steel filter, a ceramic filter, a glass sand core filter and a PEEK online filter.

4. A mobile phase bottle for liquid chromatography according to claim 1, characterized in that: The liquid adding port bottle cap (3) and the liquid inlet bottle cap (6) are screw caps or plastic caps.

5. A mobile phase bottle for liquid chromatography according to claim 4, characterized in that: The material of the liquid filling port bottle cap (3) and the liquid inlet bottle cap (6) is selected from one of PTFE, polypropylene and Teflon.

6. A mobile phase bottle for liquid chromatography according to claim 1, characterized in that: There is a certain gap between the contact surfaces of the liquid inlet pipe (4) and the liquid inlet bottle cap (6) for ventilation.

7. A high performance liquid chromatography system, comprising the mobile phase bottle for liquid chromatography according to any one of claims 1 to 6, characterized in that: The invention also comprises a pump (7), an automatic sample injector (8), a chromatographic column (9), a detector (10) and a data acquisition and processing system (11), wherein the output end of the liquid inlet tube (4) in the mobile phase bottle for liquid chromatography is connected to the input end of the pump (7), the output end of the pump (7) is connected to the input end of the automatic sample injector (8), the output end of the automatic sample injector (8) is connected to the input end of the chromatographic column (9), the output end of the chromatographic column (9) is connected to the input end of the detector (10), and the output end of the detector (10) is connected to the data acquisition and processing system (11).

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