Multi-channel primer synthesizer

Abstract

The utility model discloses a multichannel primer synthesizer which comprises a working cabin, the interior of the working cabin is divided into a synthesis cabin and a liquid injection control cabin, and a synthesis carrier for placing a synthesis column, a translation driving mechanism capable of driving the synthesis carrier to translate along the X direction and a liquid injection device are arranged in the synthesis cabin; a plurality of reagent bottles are arranged on the working cabin, a plurality of electromagnetic valves are arranged in the liquid injection control cabin, the reagent bottles are connected with the electromagnetic valves through pipelines, the electromagnetic valves are connected with the liquid injection device through pipelines, the synthesis carrier is driven by the translation driving mechanism to translate to the position below the liquid injection device, and different reagents are injected into the synthesis column through the liquid injection device; the synthesis cabin is provided with a front door, one side of the front door is rotationally connected with the cabin wall of the synthesis cabin, and the other side of the front door is buckled to the cabin wall of the synthesis cabin through a locking mechanism. According to the utility model, the problems of quantity and scale required in a medium synthesis process can be solved; the synthesis with the quantity of 1 to 12 and the scale of 5noml to 200umo l level is supported; and the synthesis process is not influenced by the external environment.

Description

Technical Field

[0001] The utility model belongs to the technical field of gene synthesis supporting equipment, and particularly relates to a multi-channel primer synthesizer. Background Art

[0002] There are many types and sizes of primer synthesis equipment at present. Among them, the synthesizer for medium synthesis scale can only perform primer synthesis on a single number and a single size of synthesis column during the synthesis process, so there is a problem that the number and scale of the required synthesis column cannot meet the actual needs. In addition, there are also problems such as large size of the synthesizer, high energy consumption and low efficiency. Moreover, since the liquid injection device of the existing synthesizer is externally exposed to the external environment, the synthesizer is highly dependent on the external environment.

[0003] Therefore, according to the above requirements, we hope to study a multi-channel primer synthesizer that can solve the above problems. Utility Model Content

[0004] The main technical problem solved by the utility model is to provide a multi-channel primer synthesizer, which can solve the quantity and scale problems required in the medium synthesis process; supports the synthesis of 1-12 quantities and 5noml-200umol scales; and the synthesis process is not affected by the external environment.

[0005] In order to solve the above technical problems, a technical solution adopted by the utility model is as follows: the utility model provides a multi-channel primer synthesizer, including a working cabin, wherein the working cabin is divided into a synthesis cabin and a liquid injection control cabin, wherein a synthesis carrier for placing a synthesis column, a translation driving mechanism capable of driving the synthesis carrier to translate along the X direction and a liquid injection device are arranged in the synthesis cabin, and the synthesis column is detachably connected to the synthesis carrier;

[0006] The working cabin is provided with a plurality of reagent bottles, the liquid injection control cabin is provided with a plurality of solenoid valves, the reagent bottles are connected to the solenoid valves through pipelines, the solenoid valves are connected to the liquid injection device through pipelines, the synthetic carrier is driven to translate to the bottom of the liquid injection device through the translation driving mechanism, and different reagents are injected into the synthetic column through the liquid injection device;

[0007] The synthesis cabin is provided with a front door, one side of the front door is rotatably connected to the bulkhead of the synthesis cabin, and the other side of the front door is buckled with the bulkhead of the synthesis cabin through a locking mechanism.

[0008] Furthermore, a C-shaped bottle rack is provided on the top of the working cabin, and the reagent bottles are arranged at intervals on the C-shaped bottle rack.

[0009] Furthermore, the liquid pumping device includes a liquid pumping pipe, and the solenoid valve is connected to the corresponding liquid pumping pipe.

[0010] Furthermore, the synthetic carrier includes a carrier base and a plurality of carrier adjustment handles, the carrier base is provided with a long strip groove, the carrier adjustment handles pass through the side wall of the carrier base and the ends of the carrier adjustment handles can be pressed against the synthetic column so that the synthetic column can be detachably connected to the carrier base.

[0011] Furthermore, one side of the synthetic carrier is also connected to a waste liquid collection chamber, a mounting block is provided in the waste liquid collection chamber, the mounting block is provided with a plurality of channels, one end of the channel is connected to an external gas source and a cleaning liquid.

[0012] Furthermore, a hook is provided at the upper end of the front door, and the locking mechanism is fixed on the bulkhead of the synthetic cabin. The locking mechanism includes a locking drive cylinder, a fixed block, a moving block, a stop column and a pin shaft. The fixed block is fixed on the bulkhead of the synthetic cabin, and the push rod of the locking drive cylinder is connected to the moving block. The fixed block is provided with a guide groove, and the pin shaft is installed on the moving block. The moving block is located inside the fixed block and the pin shaft can move along the knife groove. The stop column is installed on the side of the moving block away from the locking drive cylinder, and the hook can hook the stop column.

[0013] Furthermore, the front door is provided with a front perspective window, and the upper part of the synthetic cabin is provided with an upper perspective window.

[0014] Furthermore, a sealing structure is provided on the front door and the bulkhead of the synthesis cabin, and the front door can be sealingly buckled with the synthesis cabin.

[0015] Furthermore, a damping structure is provided between the front door and the bulkhead of the synthetic cabin.

[0016] The beneficial effects of the utility model are:

[0017] 1. The utility model has a compact and ingenious structure. The liquid injection device and synthesis vehicle are all built into the synthesis cabin, that is, the synthesis cabin and the liquid injection control cabin are built with various devices, which can avoid the influence of the external environment and solve the problem of the synthesizer's high dependence on the external environment;

[0018] 2. Solve the problems of large size, high energy consumption and low efficiency of the synthesizer;

[0019] 3. This device can achieve the synthesis of 1-12 quantities and 5-200umol scale;

[0020] 4. High degree of automation, no need for personnel supervision throughout the process, and can be expanded to support online detection;

[0021] 5. The synthesis column of the synthesis carrier is easy to replace, making it more compatible with various specifications of synthesis columns and synthesis requirements on the market;

[0022] 6. The built-in waste liquid collection chamber has cleaning and purging functions to solve the problems of crystallization and dripping during the synthesis process, and to solve the phenomenon of insufficient or no beating caused by crystallization during the synthesis process. Purging can solve the problems caused by dripping during the synthesis process.

[0023] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is the appearance diagram of the utility model;

[0025] Figure 2 It is one of the structural schematic diagrams of the utility model;

[0026] Figure 3 This is the second structural diagram of the utility model;

[0027] Figure 4 It is a schematic diagram of the internal structure of the utility model (the liquid pumping device and the translation drive mechanism are not shown);

[0028] Figure 5 This is one of the structural schematic diagrams of the synthetic carrier of the utility model;

[0029] Figure 6 is a cross-sectional view of the synthetic carrier of the utility model;

[0030] Figure 7 This is the second structural schematic diagram of the synthetic carrier of the utility model;

[0031] Figure 8 This is one of the structural schematic diagrams of the locking mechanism of the utility model;

[0032] Fig. 9 This is the second structural diagram of the locking mechanism of the utility model (the fixing block is not installed);

[0033] The reference numerals are as follows:

[0034] Synthetic column 10;

[0035] Working cabin 1, C-type bottle rack 11;

[0036] Composite cabin 2, front door 21, hook 211, front perspective window 212, upper perspective window 22, damping structure 23;

[0037] Liquid injection control cabin 3, solenoid valve 31;

[0038] Composite carrier 4, carrier base 41, carrier adjustment handle 42, waste liquid collection chamber 43, collection chamber height adjustment handle 431, mounting block 44, channel 441;

[0039] Translation drive mechanism 5;

[0040] Liquid pumping device 6;

[0041] Reagent bottle 7;

[0042] Locking mechanism 8, locking drive cylinder 81, fixed block 82, moving block 83, blocking column 84, pin shaft 85. DETAILED DESCRIPTION

[0043] The following is a specific embodiment of the present invention, and those skilled in the art can easily understand the advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented in other different ways, that is, different modifications and changes can be made without departing from the scope disclosed by the present invention.

[0044] Embodiment: A multi-channel primer synthesizer, such as Figures 1 to 9 As shown, it comprises a working cabin 1, which is divided into a synthesis cabin 2 and a liquid injection control cabin 3. The synthesis cabin is provided with a synthesis carrier 4 for placing a synthesis column 10, a translation driving mechanism 5 capable of driving the synthesis carrier to translate along the X direction, and a liquid injection device 6. The synthesis column is detachably connected to the synthesis carrier;

[0045] The working cabin is provided with a plurality of reagent bottles 7, and the liquid injection control cabin is provided with a plurality of solenoid valves 31. The reagent bottles are connected to the solenoid valves through pipelines, and the solenoid valves are connected to the liquid injection device through pipelines. The synthetic carrier is driven to translate to the bottom of the liquid injection device through the translation driving mechanism, and different reagents are injected into the synthetic column through the liquid injection device;

[0046] like Figures 1 to 3 As shown, the synthetic cabin is provided with a front door 21, one side of the front door is rotatably connected to the wall of the synthetic cabin, and the other side is buckled to the wall of the synthetic cabin through a locking mechanism 8. In this embodiment, the translation drive mechanism is a linear drive module, which is a known technology and will not be described in detail, and can drive the synthetic vehicle to translate accurately.

[0047] In this embodiment, Figure 1 As shown, a C-shaped bottle rack 11 is provided on the top of the working cabin, and the reagent bottles are arranged at intervals on the C-shaped bottle rack, which is beautiful and saves space.

[0048] In this embodiment, Figure 4As shown, the liquid pumping device 6 includes a liquid pumping tube; the solenoid valve is connected to the corresponding liquid pumping tube. The reagents in different reagent bottles are finally pumped into the synthesis column through the liquid pumping tube under the control of the solenoid valve.

[0049] In this embodiment, Figures 5 to 7 As shown, the synthetic carrier 4 includes a carrier base 41 and a plurality of carrier adjustment handles 42, wherein the carrier base is provided with a long strip groove, and the carrier adjustment handles pass through the side wall of the carrier base and the ends of the carrier adjustment handles can be pressed against the synthetic column so that the synthetic column can be detachably connected to the carrier base, so as to facilitate the disassembly and replacement of the synthetic column.

[0050] In this embodiment, Figure 5 As shown, a waste liquid collection cabin 43 is also connected to one side of the synthetic carrier, and a mounting block 44 is provided in the waste liquid collection cabin, and the mounting block is provided with a plurality of channels 441, and one end of the channel is externally connected to an air source and a cleaning liquid. A collection cabin height adjustment handle is also provided at the bottom of the waste liquid collection cabin, and its front end can be fixed on the carrier base. A waist-shaped hole extending in a vertical direction is provided at the bottom of the waste liquid collection cabin, and the collection cabin height adjustment handle can move along the waist-shaped hole to adjust the height of the waste liquid collection cabin.

[0051] In this embodiment, Figure 8 and Fig. 9 As shown, a hook 211 is provided at the upper end of the front door, the locking mechanism is fixed on the bulkhead of the synthetic cabin, the locking mechanism 8 comprises a locking drive cylinder 81, a fixed block 82, a moving block 83, a stopper 84 and a pin 85, the fixed block is fixed on the bulkhead of the synthetic cabin, the push rod of the locking drive cylinder is connected to the moving block, the fixed block is provided with a guide groove, the pin is installed on the moving block, the moving block is located inside the fixed block and the pin can move along the knife groove, the stopper is installed on the side of the moving block away from the locking drive cylinder, and the hook can hook the stopper. Preferably, a sensor is also provided at the locking structure for sensing the position of the front door.

[0052] In this embodiment, Figure 1 As shown, the front door is provided with a front perspective window 212, and the upper part of the synthetic cabin is provided with an upper perspective window 22.

[0053] In this embodiment, a sealing structure is provided on the front door and the bulkhead of the synthesis cabin, and the front door can be sealingly buckled with the synthesis cabin.

[0054] In this embodiment, Figure 3 As shown, a damping structure 23 is further provided between the front door and the bulkhead of the synthetic cabin. There can be many kinds of damping structures, as long as the front door can be hovered after opening a certain angle.

[0055] The front perspective window and front door can be opened quickly. By opening the front door, you can take out and place the synthetic column, clean the needle, and perform routine maintenance. Since the opening angle of the front door is very large, it is very convenient to operate.

[0056] The front perspective window and the upper perspective window provide a good observation field of view, and the changes in the synthesis process can be well viewed with the light source inside the synthesis cabin.

[0057] The synthesis column is inserted into the carrier. The synthesizer supports the synthesis of primers with 12 channels within 15mm without interfering with each other. Synthesis of 6 columns within 30mm is synchronized without interfering with each other. It supports mixing of large and small synthesis columns. The overall synthesis process is completed in the synthesis cabin, which is a sealed design, and the liquid injection device is built into the synthesis cabin, and there is always inert gas protection inside. Therefore, the environmental dependence of the synthesis process is low.

[0058] The injection control cabin provides an installation location for the solenoid valve that controls the micro-flow, while protecting the solenoid valve from corrosive liquids and isolating it from the electrical control part. This part can provide accurate reagent injection of more than 1uml through internal algorithm control. It is a key component for the accurate synthesis of the synthesizer.

[0059] Bottle racks provide a hanging location for reagent bottles.

[0060] The solution of using various devices built into the synthesis cabin and the liquid injection control cabin can avoid the influence of the external environment and make the structure simple.

[0061] The synthesis column of the synthesis carrier is easy to replace, making it more compatible with various specifications of synthesis columns and synthesis requirements on the market.

[0062] The built-in waste liquid collection chamber has a combination of cleaning and purging functions to solve the problems of crystallization and dripping during the synthesis process, and to solve the phenomenon of insufficient or no beating caused by crystallization during the synthesis process. Purging solves the problem caused by dripping during the synthesis process.

[0063] In a normal synthesis process, when the external electrical fluid supply is good, the general process is as follows: first open the front door, select the appropriate synthesis column according to the synthesis requirements, install the synthesis column and synthesis carrier; import the program, close the front door and start the synthesis. At the beginning of the synthesis, the synthesizer will self-check whether all indicators are normal. Then the synthesizer will perform the synthesis step by step according to the process requirements, including the translation drive mechanism driving the synthesis carrier to the bottom of the liquid injection device, and the various required reagents are injected into each synthesis column one by one; and the waste liquid dripping from the liquid injection device will fall into the waste liquid collection chamber, and the external cleaning liquid and gas will clean and blow the liquid injection tube. If there is a problem in the synthesis process, the machine will automatically alarm, and it will also prompt that the synthesis is completed after the synthesis is completed.

[0064] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure made using the contents of the specification and drawings of the present invention, or directly or indirectly used in other related technical fields, is also included in the patent protection scope of the present invention.

Claims

1. A multi-channel primer synthesizer, characterized in that: The invention comprises a working cabin (1), wherein the working cabin is divided into a synthesis cabin (2) and a liquid injection control cabin (3), wherein a synthesis carrier (4) for placing a synthesis column (10), a translation driving mechanism (5) capable of driving the synthesis carrier to translate along the X direction, and a liquid injection device (6) are arranged in the synthesis cabin, and the synthesis column is detachably connected to the synthesis carrier; The working cabin is provided with a plurality of reagent bottles (7), the liquid injection control cabin is provided with a plurality of electromagnetic valves (31), the reagent bottles are connected to the electromagnetic valves via pipelines, the electromagnetic valves are connected to the liquid injection device via pipelines, the synthetic carrier is driven to translate to the bottom of the liquid injection device via a translation driving mechanism, and different reagents are injected into the synthetic column via the liquid injection device; The synthesis cabin is provided with a front door (21), one side of the front door is rotatably connected to the cabin wall of the synthesis cabin, and the other side of the front door is buckled with the cabin wall of the synthesis cabin through a locking mechanism (8).

2. The multichannel primer synthesizer according to claim 1, characterized in that: A C-shaped bottle rack (11) is provided on the top of the working cabin, and the reagent bottles are arranged on the C-shaped bottle rack at intervals.

3. The multi-channel primer synthesizer according to claim 1, characterized in that: The liquid pumping device (6) comprises a plurality of liquid pumping tubes, and the solenoid valve is connected to the corresponding liquid pumping tubes.

4. The multi-channel primer synthesizer according to claim 1, characterized in that: The synthetic carrier (4) comprises a carrier base (41) and a plurality of carrier adjustment handles (42), wherein the carrier base is provided with a long strip groove, and the carrier adjustment handles pass through the side wall of the carrier base and the ends of the carrier adjustment handles can be pressed against the synthetic columns so that the synthetic columns can be detachably connected to the carrier base.

5. The multi-channel primer synthesizer according to claim 1, characterized in that: One side of the synthetic carrier is also connected to a waste liquid collection chamber (43), a mounting block (44) is provided in the waste liquid collection chamber, and the mounting block is provided with a plurality of channels (441), one end of the channel is connected to an external gas source and a cleaning liquid.

6. The multi-channel primer synthesizer according to claim 1, characterized in that: A hook (211) is provided at the upper end of the front door, the locking mechanism is fixed on the bulkhead of the synthetic cabin, the locking mechanism (8) comprises a locking drive cylinder (81), a fixed block (82), a moving block (83), a stop column (84) and a pin (85), the fixed block is fixed on the bulkhead of the synthetic cabin, the push rod of the locking drive cylinder is connected to the moving block, the fixed block is provided with a guide groove, the pin is installed on the moving block, the moving block is located inside the fixed block and the pin can move along the knife groove, the stop column is installed on the side of the moving block away from the locking drive cylinder, and the hook can hook the stop column.

7. The multi-channel primer synthesizer according to claim 1, characterized in that: The front door is provided with a front perspective window (212), and the upper part of the synthesis cabin is provided with an upper perspective window (22).

8. The multi-channel primer synthesizer according to claim 1, characterized in that: The front door and the bulkhead of the synthesis cabin are provided with a sealing structure, and the front door can be sealed and buckled with the synthesis cabin.

9. The multi-channel primer synthesizer according to claim 1, characterized in that: A damping structure (23) is also provided between the front door and the bulkhead of the synthetic cabin.

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