Novel soil root-knot nematode separating device
By using a warm light to heat the samples in the soil root knot nematode separation device, the active movement of nematodes is promoted, and combined with the sliding pulling plate and partition control hose opening and closing, the problems of low separation efficiency, cumbersome operation and large space occupation in the prior art are solved, and efficient and simple nematode separation is achieved.
Patent Information
- Application Number
- CN202421173452.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-27
- Publication Date
- 2025-03-11
- Estimated Expiration
- 2034-05-27
AI Technical Summary
The existing Bellman funnel nematode separation method has low separation efficiency and long separation cycle. It is complicated to operate when separating multiple samples at the same time and takes up a large space.
A new type of soil root knot nematode separation device is designed to increase the sample temperature by installing a warm light in the test box, which promotes the active movement of nematodes and shortens the separation time. At the same time, the hose opening and closing of multiple groups of nematode separation instruments is controlled by slidable pulling plates and partitions, simplifying operation and reducing space occupation.
It improves the efficiency and speed of nematode separation, simplifies the operation process, reduces space occupation, and is suitable for simultaneous separation of multiple samples.
Smart Images
Figure CN222583401U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of root-knot nematode separation, in particular to a novel soil root-knot nematode separation device. Background Art
[0002] The Baermann funnel method is a classic nematode separation method. The specific operation of this method is: place the funnel above a small beaker, connect a rubber tube pointing to the inside of the small beaker to the bottom of the funnel, and use a water stop clamp to clamp the rubber tube; place a layer of wire mesh and gauze in the funnel, and then place a layer of nematode filter paper; during the test, evenly spread the soil sample or chopped plant material on the filter paper, and then add water to immerse the sample. After separating at room temperature for 12 to 24 hours, open the water stop clamp again, so that the filtrate accumulated in the rubber tube flows from the rubber tube to the small beaker, and finally the filtrate collected in the small beaker is made into a slide for counting. This method mainly uses the negative phototropism and gravity of nematodes, the active peristalsis of nematodes, and the natural sinking effect of nematodes to separate the nematodes from the soil and enter the filtrate.
[0003] However, when the funnel, rubber tube, water stop clamp and small beaker are used as a set of nematode separation instruments, it takes 12 to 24 hours to complete the separation, and the separation efficiency is low.
[0004] In addition, in the nematode separation test, multiple samples often need to be separated at the same time, so the test personnel need to operate the water-stop clamps of multiple groups of nematode separation instruments separately during the separation process, which is cumbersome. Space for operating the water-stop clamps needs to be reserved between each group of nematode separation instruments, otherwise it is easy to bump into each other and take up a lot of space. Utility Model Content
[0005] The utility model solves the problems of low separation efficiency and long separation cycle when using a funnel, a rubber hose, a water stop clamp and a small beaker as a nematode separation instrument, and the problem that when using the nematode separation instrument to separate multiple samples at the same time, the operation process is cumbersome and multiple groups of nematode separation instruments need to occupy a large amount of space. A new soil root-knot nematode separation device is provided to solve the technical problem. A warm light is installed in the test box to increase the sample temperature in the funnel and promote the nematodes to actively move to the hose at the bottom of the funnel, which can shorten the time required for nematode separation and improve the separation efficiency. The slidable pull-out plate and the partition cooperate with each other in the test box to control the opening and closing of each hose at the same time, so that multiple groups of nematode separation instruments can be conveniently controlled at the same time, simplifying the operation process. When the pull-out plate and the partition cooperate to control the opening and closing of the hose, there is no need to reserve space between each group of nematode separation instruments to operate the water stop clamp and take the beaker, which can reduce space occupation.
[0006] In order to solve the above technical problems, the technical solution of the utility model is as follows:
[0007] A novel soil root-knot nematode separation device comprises a plurality of nematode separation instruments and a test box for accommodating the plurality of nematode separation instruments. Each group of the nematode separation instruments comprises a beaker located at the lower layer of an inner cavity, a funnel located at the upper layer of the beaker, and a hose sleeved at the bottom end of the funnel. The top of the test box is open and movably connected to a box cover for covering the open opening. A warm light is provided on the side of the box cover facing the inside of the test box. Stacked horizontal partitions and push-pull plates are provided in the test box. The partitions and push-pull plates separate the inner cavity of the test box into an upper layer for placing the funnel and a lower layer for placing the funnel. The lower layer of the cup, the partition and the push-pull plate are respectively provided with a first through hole and a second through hole for each hose to pass through, and the first through hole and the second through hole for the same hose to pass through are defined as a group of through holes, the position of the partition is fixed, and the push-pull plate is slidably matched with the test box and the partition in the horizontal direction, and the relatively sliding push-pull plate and the partition are switched between a first state in which two through holes in the same group are aligned and a second state in which the two through holes in the same group are misaligned and clamp the hose, and a locking component is also provided between the push-pull plate and the partition for locking the relative position of the push-pull plate and the partition in the second state,
[0008] The side of the test box corresponding to the push-pull plate is provided with an avoidance hole for the push-pull plate to slide through, and the side of the test box corresponding to the lower layer of the inner cavity is also provided with an opening to facilitate taking and placing the beaker in the lower layer of the inner cavity.
[0009] Preferably, the locking assembly includes an internal threaded seat connected to the push-pull plate and a stud threadedly matched with the internal threaded seat, the screwing direction of the stud is parallel to the sliding direction of the push-pull plate, and one end of the stud abuts against the partition.
[0010] Preferably, the locking assembly includes a positioning pin and a pin hole formed on the push-pull plate and the partition. When the push-pull plate and the partition are in the second state, the pin holes of the push-pull plate and the partition are aligned for the positioning pin to be inserted.
[0011] Preferably, the first through hole and the second through hole are both rectangular holes, and when the push-pull plate and the partition are in the second state, the first through hole and the second through hole are parallel to each other against the hole wall of the side of the hose.
[0012] Preferably, a pull-out box for supporting all beakers is placed at the lower layer of the inner cavity of the test box, the pull-out box is slidably matched with the test box, and the pull-out box slides into or out of the inner cavity of the test box through the side opening of the test box.
[0013] Preferably, a support plate for supporting the funnel is provided on the upper layer of the inner cavity of the test box, and the support plate is provided with a plurality of receiving holes for placing the funnel, and the diameter of each of the receiving holes is smaller than the diameter of the top end of the funnel and larger than the diameter of the bottom end of the funnel.
[0014] Preferably, the bottom end of the hose of the test box extends downward through the partition and is inserted into the mouth of the beaker.
[0015] Beneficial technical effects of the technical solution of the utility model:
[0016] (I) The test box with an open top contains multiple sets of nematode separation instruments. The beaker of the nematode separation instrument is located below the funnel, and a hose is connected to the bottom of the beaker. A warm light is installed on the side of the lid at the top of the test box facing the inside of the test box. When the nematodes need to be separated, the warm light is used to illuminate the beaker to heat up the sample inside the beaker, which promotes the nematodes to actively move downward to the bottom of the funnel and enter the hose, which can accelerate the completion of nematode separation, shorten the time required for nematode separation, and improve separation efficiency.
[0017] The test box is also equipped with a partition and a pull-out plate for separating the funnel and the beaker. The pull-out plate and the partition are provided with multiple pairs of through holes for the hoses of each group of nematode separation instruments to pass through. By controlling the relative sliding of the pull-out plate and the partition, when the through holes in the same group are misaligned, the hole walls of the two through holes in the same group can be used to clamp the hoses passing through the through holes to close the hoses. Each group of misaligned through holes can clamp the hoses of a group of nematode separation instruments respectively, that is, the opening and closing of each hose can be controlled at the same time, and the staff no longer needs to open and close the water stop clamps on each hose one by one, simplifying the operation process. In addition, a push-pull plate is used to control the opening and closing of the hoses of each group of nematode separation instruments, and there is no need to reserve space for the staff to operate the water stop clamps. Adjacent nematode separation instruments can be densely placed to reduce space occupancy.
[0018] (ii) The through hole is a rectangular hole. When the push-pull plate and the partition are in the second state, the first through hole and the second through hole are parallel to each other against the hole walls on the side of the hose. The hose is clamped by two parallel hole walls to ensure that the hose is completely clamped and closed to prevent leakage of the clamped hose.
[0019] (III) The pull-out box and the placement box are slidably matched, and all beakers are placed in the pull-out box. When the beaker needs to be transferred, the pull-out box can be directly slid into or out of the placement box, so that the beaker with the filtrate collected can be conveniently transferred. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 It shows a schematic structural diagram of a novel soil root-knot nematode separation device in an embodiment of the utility model;
[0021] Figure 2 The schematic diagram of the structure of the supporting plate, the partition plate, the push-pull plate and the nematode separation instrument in the embodiment of the utility model is shown.
[0022] Markings in the accompanying drawings:
[0023] 1-test box; 11-box cover; 111-warm light; 12-support plate; 13-pull-out box; 2-partition plate; 21-first through hole; 22-push-pull plate; 221-second through hole; 23-internal thread seat; 231-stud; 3-funnel; 31-hose; 32-beaker. DETAILED DESCRIPTION
[0024] In order to make the purpose, technical scheme and advantages of the utility model clearer, the following is a further detailed description of a novel soil root-knot nematode separation device proposed by the utility model in combination with the accompanying drawings and specific embodiments. According to the following description, the advantages and features of the utility model will be clearer. It should be noted that the accompanying drawings adopt a very simplified form and use non-precise proportions, which are only used to conveniently and clearly assist in explaining the purpose of the implementation method of the utility model. In order to make the purpose, features and advantages of the utility model more obvious and easy to understand, please refer to the accompanying drawings. It should be noted that the structure, proportion, size, etc. illustrated by the accompanying drawings of this specification are only used to match the content disclosed in the specification, so that people familiar with this technology can understand and read, and are not used to limit the limiting conditions for the implementation of the utility model, so it has no technical substantive significance. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effect that the utility model can produce and the purpose that can be achieved, should still fall within the scope of the technical content disclosed by the utility model.
[0025] The following will be combined with the attached Figure 1 , 2 The technical scheme of a novel soil root-knot nematode separation device of the utility model is described in detail with specific embodiments.
[0026] Example
[0027] like Figure 1 and 2 As shown, a novel soil root-knot nematode separation device of the present embodiment includes a plurality of nematode separation instruments and a test box 1 for accommodating the plurality of nematode separation instruments. The plurality of nematode separation instruments are evenly distributed in the test box 1. Taking one of the nematode separation instruments as an example, the nematode separation instruments all include a beaker 32 and a funnel 3 located above the beaker 32. A hose 31 is also sleeved on the bottom end of the funnel 3. The hose 31 extends downward in a vertical direction into the beaker 32, and the bottom end of the hose 31 is lower than the cup mouth of the beaker 32.
[0028] The specific structure of the test box 1 is as follows:
[0029] The top of the test box 1 is open, and the top of the test box 1 is also rotatably connected to a box cover 11 for covering the open opening. Two warm light lamps 111 are also installed side by side on the side of the box cover 11 facing the inside of the test box 1. When the nematode separation instrument is placed in the test box 1 for the nematode separation test, the hoses 31 of each group of nematode separation instruments are clamped and closed, and then the box cover 11 is closed and the warm light lamp 111 is turned on. The warm light lamp 111 is used to heat the sample in the funnel 3. Due to the characteristics of nematodes that they are not resistant to high temperatures, the nematodes can be promoted to actively move to the filtrate at the bottom of the funnel 3 and the hose 31, thereby accelerating the completion of nematode separation, shortening the time required for nematode separation, and improving separation efficiency.
[0030] Specifically, a pull-out box 13 is placed on the bottom wall of the test box 1, and an opening is also processed at a position corresponding to the pull-out box 13 on the side of the test box 1. The pull-out box 13 can slide into or out of the test box 1 from the opening. In this embodiment, multiple groups of beakers 32 of nematode separation instruments are placed in the test box 1. By pulling the test box 1, all beakers 32 can be easily taken out together, which is convenient for collecting the filtrate obtained after nematode separation.
[0031] A horizontal support plate 12 is also provided in the test box 1, and evenly distributed receiving holes are opened on the support plate 12. Each funnel 3 is placed in a receiving hole. The top diameter of the funnel 3 is larger than the diameter of the receiving hole, and the bottom diameter of the funnel 3 is smaller than the diameter of the receiving hole. Therefore, when the funnel 3 is placed in the receiving hole, the funnel 3 can be stuck in the receiving hole, that is, the support plate 12 is used to stably support each funnel 3.
[0032] In addition, the test box 1 is provided with a stacked horizontal partition 2 and a push-pull plate 22. The partition 2 is fixedly connected to the test box 1. The push-pull plate 22 slides relative to the partition 2 in the horizontal direction. A clearance hole for the push-pull plate 22 to slide is provided at a position corresponding to the push-pull plate 22 on the side of the test box 1, so as to facilitate the sliding of the push-pull plate 22 relative to the partition 2. The partition 2 and the push-pull plate 22 in this embodiment separate the inner cavity of the test box 1 into an upper layer for placing the funnel 3 and a lower layer for placing the beaker 32. The partition 2 is provided with a plurality of first through holes 21 for each hose 31 to pass through, and the push-pull plate 22 is provided with a plurality of second through holes 221 for each hose 31 to pass through. The first through hole 21 and the second through hole 221 for the same hose 31 to pass through are defined as a pair of through holes. Only when the through holes in the same group are aligned, can the hose 31 pass through the partition 2 and the push-pull plate 22 smoothly.
[0033] When the push-pull plate 22 slides, the push-pull plate 22 and the partition 2 can be switched between the first state and the second state. In the first state, the through holes in the same group are aligned with each other, the hose 31 passes through the two through holes in the same group, and is not squeezed, so that the filtrate in the hose 31 can be smoothly retained; in the second state, the first through hole 21 and the second through hole 221 in the same group are misaligned with each other, and the hose 31 passing through the first through hole 21 and the second through hole 221 is clamped, so that the hose 31 is compressed and closed, so that the filtrate in the hose 31 cannot flow through the compressed closed position. In addition, a locking component is provided between the push-pull plate 22 and the partition 2 to lock the relative position of the push-pull plate 22 and the partition 2 in the second state, so as to prevent the push-pull plate 22 and the partition 2 from returning to the first state under the elastic action of the hose 31, thereby ensuring that the push-pull plate 22 and the partition 2 can stably maintain the second state, and at the same time, the squeezed part of the hose 31 also maintains a closed state.
[0034] When the push-pull plate 22 is controlled to slide, each group of misaligned through holes can respectively clamp or loosen the hose 31 of a group of nematode separation instruments, that is, the opening and closing of each hose 31 can be controlled at the same time, and the staff no longer needs to open and close the water stop clamps on each hose 31 one by one, which simplifies the operation process. In addition, the push-pull plate 22 is used to control the opening and closing of the hose 31 of each group of nematode separation instruments, and there is no need to reserve space for the staff to operate the water stop clamps. Adjacent nematode separation instruments can be densely placed to reduce space occupancy.
[0035] Specifically, the first through hole 21 and the second through hole 221 in the present embodiment are both rectangular holes. When the push-pull plate 22 and the partition 2 are in the second state, the first through hole 21 and the second through hole 221 are parallel to each other against the hole walls on the side of the hose 31, and are both perpendicular to the sliding direction of the push-pull plate 22. When the two parallel hole walls clamp the hose 31, the spacing between the hole walls is consistent, which can ensure that the hose 31 is completely clamped and closed to avoid uneven force at the clamping position of the hose 31 and leaving gaps, thereby preventing the clamped hose 31 from leaking.
[0036] As an implementation method of this embodiment, the locking assembly includes an internal thread seat 23 connected to the push-pull plate 22 and a stud 231 threadedly matched with the internal thread seat 23, the internal thread seat 23 is connected to a side edge of the push-pull plate 22, the internal thread seat 23 is located at the position of the avoidance hole, and the internal thread seat 23 is always located outside the test box 1, when the push-pull plate 22 needs to be pulled, the internal thread seat 23 can be directly pinched, and the push-pull plate 22 is driven to slide through the internal thread seat 23. The internal thread seat 23 is provided with a threaded hole through which the stud 231 passes, and the direction of the threaded hole is parallel to the sliding direction of the push-pull plate 22, that is, the screwing-in and screwing-out direction of the stud 231 is parallel to the sliding direction of the push-pull plate 22. When the push-pull plate 22 is pulled to slide outside the test box 1 until the push-pull plate 22 and the partition 2 are in the second state, the stud 231 is screwed in until it is tightly pressed against the side of the partition 2, and the stud 231 can prevent the push-pull plate 22 from sliding toward the inside of the test box 1.
[0037] As another implementation in this embodiment, the locking assembly includes a positioning pin and a pin hole provided on the push-pull plate 22 and the partition 2. When the push-pull plate 22 and the partition 2 are in the second state, the pin holes of the push-pull plate 22 and the partition 2 are aligned for the positioning pin to be inserted. When the push-pull plate 22 and the partition 2 need to be restored to the first state, the positioning pin can be pulled out. The pin hole can be directly provided on the push-pull plate 22 and the partition 2; or two positioning blocks with pin holes can be provided in the area outside the test box 1. The two positioning blocks are fixedly connected to the side of the push-pull plate 22 and the partition 2 respectively, and the positioning pin can be directly taken and placed in the area outside the test box 1, which is more convenient to use.
[0038] Of course, the locking assembly may also be in other forms, as long as it can lock the relative positions of the push-pull plate 22 and the partition plate 2 when the two are in the second state.
[0039] In addition, the push-pull plate 22 in this embodiment is stacked on the partition 2. In order to ensure that the push-pull plate 22 does not tilt relative to the partition 2 when sliding, the two opposite inner side walls of the test box 1 in this embodiment are fixedly connected to limit blocks, which are located above the push-pull plate 22. The limit blocks cooperate with the partition 2 so that the push-pull plate 22 can only slide in the horizontal direction. However, it should be understood that the push-pull plate 22 can also be installed below the partition 2.
[0040] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0041] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.
Claims
1. A novel soil root-knot nematode separation device, characterized in that: The invention comprises a plurality of groups of nematode separation instruments and a test box for accommodating the plurality of groups of nematode separation instruments, each group of the nematode separation instruments comprises a beaker located at the lower layer of the inner cavity, a funnel located at the upper layer of the beaker, and a hose sleeved on the bottom end of the funnel, the top of the test box is open and movably connected with a box cover covering the open opening, a warm light is arranged on the side of the box cover facing the inside of the test box, and stacked horizontal partitions and push-pull plates are arranged in the test box, the partitions and push-pull plates separate the inner cavity of the test box into an upper layer for placing the funnel and a lower layer for placing the beaker, and the The partition and the push-pull plate are respectively provided with a first through hole and a second through hole for each hose to pass through, and the first through hole and the second through hole for the same hose to pass through are defined as a group of through holes. The position of the partition is fixed, and the push-pull plate is slidably matched with the test box and the partition in the horizontal direction. The relatively sliding push-pull plate and the partition switch between a first state in which two through holes in the same group are aligned and a second state in which the two through holes in the same group are misaligned and clamp the hose. A locking component is also provided between the push-pull plate and the partition for locking the relative position of the push-pull plate and the partition in the second state. The side of the test box corresponding to the push-pull plate is provided with an avoidance hole for the push-pull plate to slide through, and the side of the test box corresponding to the lower layer of the inner cavity is also provided with an opening to facilitate taking and placing the beaker in the lower layer of the inner cavity.
2. A novel soil root-knot nematode separation device as claimed in claim 1, characterized in that: The locking assembly includes an internal thread seat connected to the push-pull plate and a stud threadedly matched with the internal thread seat. The screwing direction of the stud is parallel to the sliding direction of the push-pull plate, and one end of the stud abuts against the partition.
3. A novel soil root-knot nematode separation device as claimed in claim 1, characterized in that: The locking assembly includes a positioning pin and a pin hole formed on the push-pull plate and the partition. When the push-pull plate and the partition are in the second state, the pin holes of the push-pull plate and the partition are aligned for the positioning pin to be inserted.
4. A novel soil root-knot nematode separation device as claimed in claim 1, characterized in that: The first through hole and the second through hole are both rectangular holes. When the push-pull plate and the partition plate are in the second state, the first through hole and the second through hole are parallel to each other against the hole wall of the side of the hose.
5. A novel soil root-knot nematode separation device as claimed in claim 1, characterized in that: A pull-out box for supporting all beakers is placed at the lower layer of the inner cavity of the test box. The pull-out box is slidably matched with the test box, and the pull-out box slides into or out of the inner cavity of the test box through the side opening of the test box.
6. A novel soil root-knot nematode separation device as claimed in claim 1, characterized in that: The upper layer of the inner cavity of the test box is provided with a support plate for supporting the funnel, and the support plate is provided with a plurality of receiving holes for placing the funnel, and the diameter of each receiving hole is smaller than the diameter of the top end of the funnel and larger than the diameter of the bottom end of the funnel.
7. A novel soil root-knot nematode separation device as claimed in claim 1, characterized in that: The bottom end of the hose of the test box extends downward through the partition and is inserted into the mouth of the beaker.