Sample pretreatment filling device of flow cytometer

Abstract

The utility model discloses a sample pretreatment filling device for a flow cytometer, which is characterized in that the output end of a solution pump is connected with a solution output pipe, the rear end of the solution output pipe is connected with a solution output ring, the solution output ring is connected with a sample pipe, the upper end of the sample pipe is communicated with a sample input pipe, and the lower end of the sample input pipe is communicated with a sample outlet. According to the sample pretreatment filling device for the flow cytometer, the situation that in the prior art, in the actual use process, a sample needs to be filled with a solution, the solution is conveniently fed into the flow cytometer to be detected after being mixed, and sample treatment needs a long time is improved, the solution in the sample box can be automatically mixed by using the scheme, the mixing effect is high, and the operation is convenient. The device can automatically work after the concentration is set, the working efficiency is high, the automation degree is high, the rear end can directly send a sample solution into the flow cytometer through the output pump, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a flow cytometer sample pretreatment and filling device. Background Art

[0002] At present, China Patent No.: CN201910608500.6 discloses a miniature cell culture online observation instrument, which includes a workbench, a micro-manipulation column is arranged on the workbench, a cross arm is arranged on the micro-manipulation column, a lighting device is arranged on the cross arm, a culture dish is arranged on the table of the workbench, and a sample is placed in the culture dish. The bottom of the workbench is a closed box, a miniature inverted microscope is arranged in the closed box, and an automatic focus adjustment frame is arranged under the miniature inverted microscope. The culture dish is connected to the liquid storage and transmitter through a liquid guide tube, and there is a wireless transmitting antenna on the inverted fluorescence microscope. The back of the workbench is provided with an antenna for wireless transmission, which is used for wireless transmission of recorded experimental data. The product of the present invention has the characteristics of small size, can be placed in a conventional incubator for real-time and in-situ detection of cell morphology, and the results can be transmitted to a computer or mobile phone in real time, which is convenient for the experimenter to observe even if necessary; the cells can also be stimulated by adjusting the intensity and wavelength range of different light sources or adding other liquids, and the magnification of the objective lens can be automatically adjusted to ensure that the detected samples are imaged at different magnifications. However, in actual use, the prior art still has the problem that the sample needs to be mixed with solution before being sent to the flow cytometer for detection, and the sample processing takes a relatively long time. Utility Model Content

[0003] Therefore, in view of the above problems, the utility model proposes a flow cytometer sample pretreatment filling device, which solves the technical problems in the prior art that samples still need to be filled with solution and mixed before being sent to the flow cytometer for detection during actual use, and sample processing requires a relatively long time.

[0004] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a flow cytometer sample pretreatment filling device, which structure includes a main body, a control panel, a sample input tube, a solution input tube, and a sample output tube. The control panel is arranged on the front side of the main body, the sample input tube and the solution input tube are arranged above the control panel, and the sample output tube is arranged on the left side of the main body. The main body also includes a solution tank, a solution pump, a solution output tube, a sample tube, a solution output ring, an ultrasonic mixer, a control valve, a magnetic stirrer, and a sample output pump. The sample input tube is connected to the solution tank, the lower part of the solution tank is connected to the solution pump, the output end of the solution pump is connected to the solution output tube, the rear end of the solution output tube is connected to the solution output ring, the solution output ring is connected to the sample tube, the upper end of the sample tube is communicated with the sample input tube, the lower part of the sample input tube is connected to a connecting tube, the control valve is arranged in the middle part of the connecting tube, the ultrasonic mixer is arranged above the control valve, the magnetic stirrer is arranged at the lower part of the connecting tube, and the end of the connecting tube is connected to the sample output pump.

[0005] Furthermore, a plurality of output holes are arranged inside the solution output ring, and the output holes are connected to the inner side of the middle part of the sample input tube.

[0006] Furthermore, a stirring metal rod is arranged inside the connecting pipe above the magnetic stirrer.

[0007] Furthermore, the stirring metal tube is rotatably connected to the connecting tube via a rotating shaft.

[0008] Furthermore, the diameter of the connecting pipe is 10-20 mm.

[0009] Furthermore, a transparent panel for monitoring the amount of remaining solution is provided on the main body at the front side of the solution tank.

[0010] By adopting the above-mentioned technical scheme, the beneficial effects of the utility model are as follows: the flow cytometer sample pretreatment filling device improves the existing technology. In actual use, the sample still needs to be filled with solution and mixed before being sent to the flow cytometer for detection, and the sample processing requires a relatively long time. The use of this scheme can automatically mix the sample box solution, the mixing effect is high, the concentration can be set and then work automatically, the work efficiency is high, and the degree of automation is high. The back end can directly send the sample solution into the flow cytometer through the output pump, thereby improving the work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a structural schematic diagram of the utility model;

[0012] Figure 2 It is a schematic diagram of the internal structure of the utility model.

[0013] In the figure: 1. main body; 2. control panel; 3. sample input tube; 4. solution input tube; 5. sample output tube; 11. solution tank; 12. solution pump; 13. solution output tube; 14. sample tube; 15. solution output ring; 16. ultrasonic mixer; 17. control valve; 18. magnetic stirrer; 19. sample output pump. DETAILED DESCRIPTION

[0014] The utility model is now further described in conjunction with the accompanying drawings and specific implementation methods.

[0015] refer to Figure 1-Figure 2 The present embodiment provides a flow cytometer sample pretreatment filling device, which structure includes a main body 1, a control panel 2, a sample input tube 3, a solution input tube 4, and a sample output tube 5. The control panel 2 is arranged on the front side of the main body 1, and the sample input tube 3 and the solution input tube 4 are arranged above the control panel 2. The sample output tube 5 is arranged on the left side of the main body 1. The main body 1 also includes a solution tank 11, a solution pump 12, a solution output tube 13, a sample tube 14, a solution output ring 15, an ultrasonic mixer 16, a control valve 17, a magnetic stirrer 18, and a sample output pump 19. The sample input tube 3 It is connected to a solution tank 11, the lower part of the solution tank 11 is connected to a solution pump 12, the output end of the solution pump 12 is connected to a solution output pipe 13, the rear end of the solution output pipe 13 is connected to a solution output ring 15, the solution output ring 15 is connected to a sample tube 14, the upper end of the sample tube 14 is connected to a sample input pipe 3, the lower part of the sample input pipe 3 is connected to a connecting pipe, a control valve 17 is arranged in the middle part of the connecting pipe, an ultrasonic mixer 16 is arranged above the control valve 17, a magnetic stirrer 18 is arranged at the lower part of the connecting pipe, and the end of the connecting pipe is connected to a sample output pump 19.

[0016] A plurality of output holes are arranged inside the solution output ring 15 , and the output holes are connected to the inner side of the middle portion of the sample input tube 3 .

[0017] A stirring metal rod is arranged inside the connecting pipe above the magnetic stirrer 18 .

[0018] The stirring metal tube is rotatably connected to the connecting tube via a rotating shaft.

[0019] The diameter of the connecting pipe is 10-20 mm.

[0020] A transparent panel for monitoring the amount of remaining solution is provided on the main body 1 at the front side of the solution tank 11 .

[0021] The flow cytometer sample pretreatment and filling device improves the existing technology. In actual use, the sample still needs to be filled with solution and mixed before being sent to the flow cytometer for detection, and the sample processing requires a relatively long time. The use of this solution can automatically mix the sample box solution with a high mixing effect, and the concentration can be set to work automatically, with high work efficiency and high degree of automation. The back end can directly send the sample solution into the flow cytometer through an output pump, thereby improving work efficiency.

[0022] The above shows and describes the basic principles and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is limited by the attached claims rather than the above description, and it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0023] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A flow cytometer sample pretreatment filling device, characterized in that: Its structure includes a main body, a control panel, a sample input tube, a solution input tube, and a sample output tube. The control panel is arranged on the front side of the main body, the sample input tube and the solution input tube are arranged above the control panel, and the sample output tube is arranged on the left side of the main body. The main body also includes a solution tank, a solution pump, a solution output pipe, a sample tube, a solution output ring, an ultrasonic mixer, a control valve, a magnetic stirrer, and a sample output pump. The sample input pipe is connected to the solution tank, the lower part of the solution tank is connected to the solution pump, the output end of the solution pump is connected to the solution output pipe, the rear end of the solution output pipe is connected to the solution output ring, the solution output ring is connected to the sample tube, the upper end of the sample tube is connected to the sample input pipe, the lower part of the sample input pipe is connected to a connecting pipe, a control valve is arranged in the middle part of the connecting pipe, an ultrasonic mixer is arranged above the control valve, a magnetic stirrer is arranged at the lower part of the connecting pipe, and the end of the connecting pipe is connected to the sample output pump.

2. A flow cytometer sample pretreatment and filling device according to claim 1, characterized in that: A plurality of output holes are arranged inside the solution output ring, and the output holes are connected to the inner side of the middle part of the sample input tube.

3. A flow cytometer sample pretreatment and filling device according to claim 1, characterized in that: A stirring metal rod is arranged inside the connecting pipe above the magnetic stirrer.

4. A flow cytometer sample pretreatment and filling device according to claim 3, characterized in that: The stirring metal rod is rotatably connected to the connecting pipe via a rotating shaft.

5. A flow cytometer sample pretreatment and filling device according to claim 1, characterized in that: The diameter of the connecting pipe is 10-20 mm.

6. A flow cytometer sample pretreatment and filling device according to claim 1, characterized in that: A transparent panel for monitoring the amount of remaining solution is arranged on the main body at the front side of the solution tank.

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