Extraction kit for endotoxin-removing plasmids

Abstract

The utility model relates to the technical field of kits, in particular to an endotoxin-removing plasmid extraction kit which comprises a kit body, an observation mechanism used for observing reagents is arranged on the kit body, the observation mechanism comprises windows and a shielding plate, the windows are formed in the front end and the rear end of the kit body and penetrate through the kit body, and the shielding plate is arranged on the front end and the rear end of the kit body. A window is arranged in the box body, lifting grooves are formed in the positions, above the window, of the front end and the rear end of the box body, the lifting grooves are communicated with the window, the shielding plate is slidably connected into the lifting grooves, guide columns are fixed to the positions, on the left side and the right side, in the lifting grooves, and the guide columns penetrate through the shielding plate. By means of the observation mechanism, it can be guaranteed that a light shielding environment is provided for reagents, meanwhile, observation is conveniently and rapidly conducted when information such as the remaining amount of the reagents and the guarantee date in the reagent bottles needs to be observed, the tedious operation that the reagent bottles are taken out of the kit can be avoided, the influence of illumination on the reagents is reduced, and the reagent quality is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of kits, in particular to an endotoxin-removing plasmid extraction kit. Background Art

[0002] An endotoxin-free plasmid extraction kit is a reagent combination used to extract high-purity, low-endotoxin plasmid DNA from a bacterial solution. The kit usually contains multiple reagent bottles and multiple sets of matching filters and nucleic acid purification columns, but the actual box needs to provide a light-proof environment during transportation and storage to ensure that the reagents will not be exposed to light and cause quality degradation. Therefore, when observing and determining the inventory and shelf life of the reagents in the kit, it is necessary to take out the reagent bottles, which is a more troublesome operation and will cause the reagents to be exposed to light for a long time, affecting the quality of the reagents. In response to the above problems, the present application designs an endotoxin-free plasmid extraction kit. Utility Model Content

[0003] 1. Technical issues to be solved

[0004] In view of the deficiencies of the prior art, the utility model provides an endotoxin-removing plasmid extraction kit.

[0005] (II) Technical solution

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a kit for extracting endotoxin-free plasmids, comprising a box body, an observation mechanism for observing reagents provided on the box body, the observation mechanism comprising a window and a shielding plate, windows are provided at both the front and rear ends of the box body, the windows penetrate the box body, lifting grooves are provided at both the front and rear ends of the box body above the windows, the lifting grooves are communicated with the windows, the shielding plate is slidably connected in the lifting groove, guide posts are fixed on the left and right sides of the lifting groove, the guide posts pass through the shielding plate, a first spring is sleeved on the guide post, one end of the first spring is fixed to the top of the shielding plate, the other end of the first spring is fixed to the inner top wall of the lifting groove, a rotating groove is provided on the inner wall of the lifting groove, two first rotating shafts are symmetrically fixed on the rotating groove, a rotating disk is rotatably connected to the first rotating shaft, and a first annular first ring is fixed at one end of the outer wall of the rotating disk on the inner side. The gear rails are meshed with each other, and a second annular gear rail is fixed on the outer wall of the rotating disk on the left side, near the middle position, a contact column is fixed on one end of the rotating disk near the outer side, and a contact groove is provided on the baffle plate near the top position, and the two contact columns are both located in the contact groove, and a sliding groove is provided on the box body above the rotating groove, and the sliding groove is connected with the rotating groove, and a pull rod is slidably connected to the sliding groove, and a bar gear rail is fixed on the bottom of the pull rod, and the bar gear rail is meshed with the second annular gear rail, and the right end of the pull rod is fixed with a second spring, and the other end of the second spring is fixed to the right end of the sliding groove, and a pulling groove is provided at the top of the box body above the sliding groove, and a first clamping block is fixed on the top right position of the pull rod, and the first clamping block passes upward through the box body and is located in the pulling groove and is slidably connected to it, and a box cover is fixed above the box body, and a pulling mechanism for pulling the first clamping block is provided on the box cover.

[0007] Furthermore, the utility model is improved in that the pulling mechanism includes a pulling block, which is slidably connected to the inside of the box cover, and two second clamping blocks are symmetrically fixed to the bottom end of the pulling block, and the two second clamping blocks are respectively located in two pulling grooves, and the second clamping blocks are in contact with the first clamping block, and a pulling slider is fixed to the top of the pulling block, and the pulling slider passes upward through the box cover and is slidably connected thereto, and a plurality of third springs are fixed to the right end of the pulling block, and the other end of the third spring is fixed in the box cover.

[0008] In order to reduce the influence of friction, the utility model is improved in that a second rotating shaft is fixed on the contact column, and the second rotating shaft is in contact with the inner wall of the contact groove.

[0009] In order to facilitate the fixing and removal of the box cover, the utility model is improved in that the box cover is fixed to the box body by a plurality of buckle locks.

[0010] In order to facilitate installation and disassembly of the rotating disc, the utility model is improved in that the rotating disc is fixed on the first rotating shaft by bolts, and the bolts are threadedly connected to the first rotating shaft.

[0011] In order to facilitate the carrying of the reagent kit and prevent it from slipping, the utility model is improved in that handles are installed on both sides of the box body, and anti-slip grooves are provided on the handles and the top of the pulling slide block.

[0012] (III) Beneficial effects

[0013] Compared with the prior art, the utility model provides an endotoxin-removing plasmid extraction kit, which has the following beneficial effects:

[0014] The endotoxin-free plasmid extraction kit can realize convenient and quick observation when it is needed to observe the remaining amount of the reagent in the reagent bottle, the shelf life and other information while ensuring a light-proof environment for the reagent through the observation mechanism, and can avoid the tedious operation of taking the reagent bottle out of the kit, reduce the influence of light on the reagent, and ensure the quality of the reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the first main structure of the utility model;

[0016] Figure 2 It is a schematic diagram of the first partial structure of the utility model in section;

[0017] Figure 3 It is a schematic diagram of a second partial structure of the utility model in section;

[0018] Figure 4 It is a schematic diagram of the third partial structure of the utility model.

[0019] In the figure: 1, box body; 2, window; 3, baffle plate; 4, lifting slot; 5, guide column; 6, first spring; 7, rotating slot; 8, first rotating shaft; 9, rotating disk; 10, first annular rack; 11, second annular rack; 12, contact column; 13, contact slot; 14, sliding slot; 15, pull rod; 16, bar rack; 17, second spring; 18, pulling slot; 19, first clamping block; 20, box cover; 21, pulling block; 22, second clamping block; 23, pulling slider; 24, third spring; 25, second rotating shaft; 26, buckle lock; 27, handle. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See also Figure 1-4, a kit for removing endotoxin plasmid extraction, comprising a box body 1, on which an observation mechanism for observing reagents is arranged, the observation mechanism comprising a window 2 and a shielding plate 3, windows 2 are provided at both the front and rear ends of the box body 1, the window 2 runs through the box body 1, and lifting grooves 4 are provided at both the front and rear ends of the box body 1 above the window 2, the lifting grooves 4 are communicated with the windows 2, the shielding plate 3 is slidably connected in the lifting grooves 4, guide columns 5 are fixed on the left and right sides of the lifting grooves 4, the guide columns 5 pass through the shielding plate 3, a first spring 6 is sleeved on the guide column 5, one end of the first spring 6 is fixed to the top of the shielding plate 3, and the first The other end of the spring 6 is fixed to the inner top wall of the lifting groove 4, and a rotating groove 7 is provided on the inner wall of the lifting groove 4. Two first rotating shafts 8 are symmetrically fixed on the rotating groove 7, and a rotating disk 9 is rotatably connected to the first rotating shaft 8. A first annular rack 10 is fixed to the inner end of the outer wall of the rotating disk 9, and the two first annular racks 10 are meshed. A second annular rack 11 is fixed to the middle position of the outer wall of the rotating disk 9 on the left, and a contact column 12 is fixed to the outer end of the rotating disk 9. A contact groove 13 is provided on the baffle plate 3 at the top position, and the two contact columns 12 are both located in the contact groove 13. The box body 1 is located at the rotating A sliding groove 14 is provided above the movable groove 7, and the sliding groove 14 is communicated with the rotating groove 7. A pull rod 15 is slidably connected to the sliding groove 14, and a bar rack 16 is fixed to the bottom end of the pull rod 15, and the bar rack 16 is meshed with the second annular rack 11. A second spring 17 is fixed to the right end of the pull rod 15, and the other end of the second spring 17 is fixed to the right end of the sliding groove 14. The top of the box body 1 is located above the sliding groove 14 and is provided with a pulling groove 18. A first block 19 is fixed to the top right side of the pull rod 15, and the first block 19 passes upward through the box body 1 and is located in the pulling groove 18 and is slidably connected thereto. A box cover 20 is fixed, and a pulling mechanism for pulling the first card block 19 is provided on the box cover 20. The pulling mechanism includes a pulling block 21, and the pulling block 21 is slidably connected to the inside of the box cover 20. Two second card blocks 22 are symmetrically fixed to the bottom end of the pulling block 21. The two second card blocks 22 are respectively located in two pulling grooves 18. The second card blocks 22 are in contact with the first card block 19. A pulling slider 23 is fixed to the top end of the pulling block 21. The pulling slider 23 passes upward through the box cover 20 and is slidably connected thereto. A plurality of third springs 24 are fixed to the right end of the pulling block 21, and the other end of the third spring 24 is fixed in the box cover 20.

[0022] When the kit is in use, if it is necessary to observe the remaining amount of the reagent and check the warranty date and other information, the hand presses and pushes the pull slider 23 to the right, and the pull slider 23 drives the pull block 21 and the second clamping block 22 to move to the right, the third spring 24 is compressed, and the second clamping block 22 pushes the first clamping block 19 to move to the right in the pull groove 18, and the first clamping block 19 drives the pull rod 15 and the bar rack 16 to move to the right along the sliding groove 14, the second spring 17 is compressed, and the bar rack 16 engages with the second annular rack 11, thereby driving the second annular rack 11 to move to the right. The rail 11 rotates, and the second annular rack 11 drives the rotating disc 9 and the first annular rack 10 to rotate. The two first annular racks 10 are meshed, so that the two rotating discs 9 rotate simultaneously but in opposite directions. The rotating disc 9 drives the contact column 12 to rotate around the center of the rotating disc 9. The contact column 12 is located in the contact groove 13. The contact column 12 drives the baffle plate 3 to move upward. The baffle plate 3 moves upward along the guide column 5 and the lifting groove 4. The first spring 6 is compressed, and the baffle plate 3 is away from the window 2. The internal situation of the box body 1 can be seen through the window 2.

[0023] In actual use, it is found that when the contact column 12 contacts the inner wall of the contact groove 13, friction is generated to affect the upward movement of the baffle 3. In order to solve the above problem, in this embodiment, a second rotating shaft 25 is fixed on the contact column 12, and the second rotating shaft 25 contacts the inner wall of the contact groove 13.

[0024] It is found in actual use that in order to facilitate the fixing and removal of the box cover 20 and the box body 1 , in this embodiment, the box cover 20 is fixed to the box body 1 by a plurality of buckle locks 26 .

[0025] It is found in actual use that it is difficult to install or disassemble the rotating disc 9. To solve the above problem, in this embodiment, the rotating disc 9 is fixed to the first rotating shaft 8 by bolts, and the bolts are threadedly connected to the first rotating shaft 8.

[0026] In actual use, it is found that in order to facilitate the transportation of the reagent kit and prevent slipping, in this embodiment, handles 27 are installed on both sides of the box body 1, and the handles 27 and the top of the pulling slider 23 are provided with anti-slip grooves.

[0027] In order to explain in detail the possible application scenarios, technical principles, specific schemes that can be implemented, and the purposes and effects that can be achieved, the following is a detailed description of the specific embodiments listed in conjunction with the accompanying drawings. The embodiments described herein are only used to more clearly illustrate the technical solutions of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0028] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0029] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0030] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An endotoxin-free plasmid extraction kit, comprising a box body (1), characterized in that: The box body (1) is provided with an observation mechanism for observing the reagent, and the observation mechanism comprises a window (2) and a shielding plate (3). The box body (1) is provided with windows (2) at both the front and rear ends, and the windows (2) penetrate the box body (1). The box body (1) is provided with lifting grooves (4) at both the front and rear ends above the windows (2), and the lifting grooves (4) are connected to the windows (2). The shielding plate (3) is slidably connected in the lifting grooves (4), and guide posts (5) are fixed on both the left and right sides of the lifting grooves (4), and the guide posts (5) pass through the shielding plate (3). The guide posts (5) ) is sleeved with a first spring (6), one end of the first spring (6) is fixed to the top of the shielding plate (3), and the other end of the first spring (6) is fixed to the inner top wall of the lifting groove (4). A rotating groove (7) is provided on the inner wall of the lifting groove (4), and two first rotating shafts (8) are symmetrically fixed on the rotating groove (7). A rotating disk (9) is rotatably connected to the first rotating shaft (8), and a first annular rack (10) is fixed to the inner end of the outer wall of the rotating disk (9). The two first annular racks (10) are meshed, and a first gear (10) is fixed to the middle position of the outer wall of the rotating disk (9) on the left side. A second annular rack (11) is fixed, a contact column (12) is fixed on the outer end of the rotating disk (9), a contact groove (13) is provided on the shielding plate (3) at the top position, and the two contact columns (12) are both located in the contact groove (13), a sliding groove (14) is provided on the box body (1) above the rotating groove (7), the sliding groove (14) is connected to the rotating groove (7), a pull rod (15) is slidably connected to the sliding groove (14), a bar rack (16) is fixed at the bottom end of the pull rod (15), and the bar rack (16) is connected to the second annular rack (1 1) engagement, a second spring (17) is fixed to the right end of the pull rod (15), the other end of the second spring (17) is fixed to the right end of the sliding groove (14), the top end of the box body (1) is located above the sliding groove (14) and is provided with a pulling groove (18), a first clamping block (19) is fixed to the top right side of the pull rod (15), the first clamping block (19) passes upward through the box body (1) and is located in the pulling groove (18) and is slidably connected thereto, a box cover (20) is fixed above the box body (1), and a pulling mechanism for pulling the first clamping block (19) is provided on the box cover (20).

2. The endotoxin-free plasmid extraction kit according to claim 1, characterized in that: The pulling mechanism comprises a pulling block (21), the pulling block (21) is slidably connected to the inside of the box cover (20), two second clamping blocks (22) are symmetrically fixed to the bottom end of the pulling block (21), the two second clamping blocks (22) are respectively located in two pulling grooves (18), the second clamping blocks (22) are in contact with the first clamping block (19), a pulling slider (23) is fixed to the top end of the pulling block (21), the pulling slider (23) passes through the box cover (20) upwards and is slidably connected thereto, a plurality of third springs (24) are fixed to the right end of the pulling block (21), and the other end of the third spring (24) is fixed in the box cover (20).

3. The endotoxin-free plasmid extraction kit according to claim 1, characterized in that: A second rotating shaft (25) is fixed on the contact column (12), and the second rotating shaft (25) is in contact with the inner wall of the contact groove (13).

4. The endotoxin-free plasmid extraction kit according to claim 1, characterized in that: The box cover (20) is fixed to the box body (1) via a plurality of snap locks (26).

5. The endotoxin-free plasmid extraction kit according to claim 1, characterized in that: The rotating disc (9) is fixed on the first rotating shaft (8) by means of bolts, and the bolts are threadedly connected to the first rotating shaft (8).

6. The endotoxin-free plasmid extraction kit according to claim 1, characterized in that: Handles (27) are installed on both sides of the box body (1), and anti-slip grooves are provided on the top of the handles (27) and the pulling slider (23).

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