Photosynthetic bacteria preparation fermentation tank

Abstract

The utility model discloses a photosynthetic bacteria preparation fermentation tank, and belongs to the technical field of microbial fermentation. Comprising a tank body which is connected with a first light source system and a second light source system; the first light source system comprises a plurality of glass tubes, one end of each glass tube is of a closed structure, and the other end is of an open structure; the plurality of glass tubes are horizontally and uniformly distributed on the side wall of the tank body, the closed ends of the glass tubes extend into the tank body, and the open ends of the glass tubes are arranged outside the tank body; a light source and a heat dissipation structure are arranged in the glass tube; the second light source system is arranged inside the top of the tank body; an annular gas disperser is arranged at the bottom of the tank body; according to the utility model, a two-step photosynthetic bacteria fermentation process can be realized, namely the number of bacteria is quickly increased in the early stage, and photosynthetic bacteria pigment synthesis is promoted in the later stage; the problem that the fermentation efficiency is low due to the fact that the temperature in the photosynthetic bacteria fermentation tank is not easy to control and the illumination intensity is affected is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of microbial fermentation, in particular to a photosynthetic bacteria preparation fermentation tank. Background Art

[0002] Photosynthetic bacteria have been widely used in fertilizers, feed additives and environmental treatment, but low scale, unstable product quality and high cost are still factors that limit their large-scale application. At present, the disclosed photosynthetic fermentation device uses a double-layer stainless steel tank, introduces a glass tube into the fermentation tube, and adds a light source to ferment and produce photosynthetic bacteria preparations.

[0003] In this system, when the bacterial concentration is low in the early stage, the light source is set in the glass tube, and its light transmittance is still good. However, with the increase of bacterial concentration and the unique wall adhesion of photosynthetic bacteria, they will continue to adhere to the glass tube, so that in the middle and late stages of fermentation, the light intensity in the tank is seriously insufficient, which not only prolongs the fermentation cycle, but also reduces the bacterial concentration, thus failing to meet market requirements. At the same time, since the best light source for the growth of photosynthetic bacteria is an incandescent lamp, there are problems of high energy consumption and easy heat generation during the fermentation process, causing the tank temperature to rise, and the cooling system needs to be maintained between 28-32°C to ensure the normal growth of photosynthetic bacteria; at the same time, incandescent lamps can easily cause the glass tube to rupture due to heat, resulting in fermentation failure. Therefore, there is an urgent need for a low-cost, efficient and stable fermentation equipment to meet the market demand for photosynthetic bacteria. Utility Model Content

[0004] The utility model overcomes the shortcomings of the prior art and proposes a photosynthetic bacteria preparation fermentation tank, which is a two-step fermentation device designed based on the concept that photosynthetic bacteria can grow both under light anaerobically and in the dark aerobically, so as to achieve efficient, energy-saving and controllable large-scale production of photosynthetic bacteria preparations.

[0005] In order to achieve the above purpose, the utility model is implemented through the following technical solutions.

[0006] A photosynthetic bacteria preparation fermentation tank comprises a tank body, wherein a stirring device is arranged in the tank body; the tank body is connected to a first light source system and a second light source system; the first light source system comprises a plurality of glass tubes, wherein one end of the glass tubes is a closed structure and the other end is an open structure; the plurality of glass tubes are horizontally evenly distributed on the side wall of the tank body, and the closed ends of the glass tubes extend into the tank body, and the open ends of the glass tubes are arranged outside the tank body; a light source and a heat dissipation structure are arranged in the glass tubes; the length and position of the glass tubes extending into the tank body do not interfere with the stirring device; the second light source system is arranged on the inner side of the top of the tank body; an annular gas disperser is arranged at the bottom of the tank body; a plurality of vents are arranged on the annular gas disperser, and a one-way valve is arranged on the vent, and the annular gas disperser is connected to a sterile gas supply device through a pipeline.

[0007] Furthermore, the heat dissipation structure includes a cold air pipe and a cold air supply device, one end of the cold air pipe is connected to the cold air supply device, and the other end extends into the glass tube.

[0008] Furthermore, the annular gas disperser is an annular tube body, and the bottom of the annular gas disperser is connected to the inner wall of the tank bottom of the tank body through a bracket.

[0009] Furthermore, the second light source system includes a plurality of incandescent lamps, and the plurality of incandescent lamps are evenly distributed on the inner side of the top of the tank body with the axis of the tank body as the center.

[0010] Furthermore, a manhole is provided on the top of the tank.

[0011] Furthermore, the tank wall of the tank body is a double-layer structure, a feed port is arranged at the upper part of the tank body side wall, and a discharge port is arranged at the lower part of the tank body side wall; control valves are arranged on the feed port and the discharge port.

[0012] Furthermore, a pH probe, a dissolved oxygen probe and a temperature probe are arranged in the tank.

[0013] Furthermore, the stirring device includes a motor, a stirring shaft and a stirring paddle. The motor is arranged on the top of the tank body, the stirring shaft is connected to the motor and is vertically arranged inside the tank body, the stirring shaft is evenly distributed with stirring paddles from top to bottom, and the stirring paddles are staggered with the position of the glass tube.

[0014] The beneficial effects of the utility model compared with the prior art are:

[0015] The utility model is an improvement on the technical solution of the Chinese patent CN 202116543 U intelligent photosynthetic microbial fermentation tank. The utility model improves the glass tube that runs through the top and bottom of the tank body to a semi-enclosed glass tube connected to the side wall of the tank body. The semi-enclosed glass tube is staggered with the stirring blade and the length does not contact the central stirring shaft. This design of introducing a glass tube can increase the stirring resistance, and can play a role in breaking up the water vortex during stirring, so that the dispersion of the bacteria in the fermentation liquid is higher and the fermentation efficiency is improved; at the same time, the semi-enclosed glass tube is connected to the heat dissipation structure, which can effectively reduce the temperature in the tank; and the glass tube is a structure with multiple evenly distributed inside the side wall of the tank body, and the light source is evenly dispersed, dispersing the bacteria growing on the wall and extending the light intensity. At the same time, combined with the second light source system at the top, it avoids contact with the fermentation liquid and prevents the surface of the second light source system from being attached to the wall, and can work together with the first light source system to improve the light intensity in the tank.

[0016] The utility model arranges an annular gas disperser at the bottom of the tank body, that is, sterile air is evenly delivered into the tank body through the annular gas disperser, so that the gas entering the tank body is more dispersed, and the dissolved oxygen in the tank body is more uniform, more energy-saving, and highly controllable, so as to ensure the growth of photosynthetic bacteria.

[0017] The utility model can realize a two-step fermentation process of photosynthetic bacteria. In the first step, the photosynthetic bacteria are rapidly fermented under dark aerobic conditions by utilizing the characteristics of fast growth, low energy consumption and simple process. Oxygen is provided to the tank through an annular gas disperser at the bottom of the tank body, and the first light source system and the second light source system are turned off at the same time to provide conditions for dark aerobic fermentation. The number of bacteria is rapidly increased at this stage. After the first step of fermentation is completed, the gas supply system is turned off, the first and second light source systems are turned on, and the second step of light anaerobic fermentation is started to promote the synthesis of photosynthetic bacteria pigments and complete the final fermentation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the structure of the photosynthetic bacteria preparation fermentation tank of the utility model;

[0019] Figure 2 for Figure 1 AA section view of the middle tank body;

[0020] Figure 3 is a schematic diagram of the arrangement of the second light source system located on the top of the tank;

[0021] Figure 4 This is a structural diagram of the glass tube of the first light source system.

[0022] In the figure: 1. tank body; 2. annular gas disperser; 3. glass tube; 4. vent; 5. pipeline; 6. cooling air pipe; 7. incandescent lamp; 8. manhole; 9. feed inlet; 10. discharge outlet; 11. pH probe; 12. dissolved oxygen probe; 13. temperature probe; 14. motor; 15. stirring shaft; 16. stirring paddle; 17. light source. DETAILED DESCRIPTION

[0023] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail in combination with the embodiments and the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. The technical solution of the present invention is described in detail below in combination with the embodiments and the accompanying drawings, but the scope of protection is not limited thereto.

[0024] See also Figures 1 to 4 , this embodiment discloses a photosynthetic bacteria preparation fermentation tank, including a tank body 1, the tank body 1 has a larger cross-sectional radius than a conventional fermentation tank to adapt to the arrangement and installation of the internal structure. The tank wall of the tank body 1 is a double-layer structure, and a manhole 8 is arranged on the top of the tank body 1; a feed port 9 is arranged on the upper part of the side wall of the tank body 1, and a discharge port 10 is arranged on the lower part of the side wall of the tank body 1; control valves are arranged on the feed port 9 and the discharge port 10. A stirring device is arranged in the tank body 1; the stirring device includes a motor 14, a stirring shaft 15 and a stirring paddle 16, the motor 14 is arranged on the top of the tank body 1, the stirring shaft 15 is connected to the output end of the motor 14 and is vertically arranged at the central axis inside the tank body 1, the stirring shaft 15 is evenly distributed with stirring paddles 16 from top to bottom, and a pH probe 11, a dissolved oxygen probe 12 and a temperature probe 13 are arranged in the tank body 1. The stirring of the stirring paddle 16 promotes the uniformity of the fermentation liquid in the tank body 1, and the fermentation environment indicators in the tank body 1, such as pH, dissolved oxygen and temperature, are monitored by the pH probe 11, the dissolved oxygen probe 12 and the temperature probe 13.

[0025] The tank body 1 is connected to a first light source system and a second light source system; the first light source system includes a plurality of glass tubes 3 and a light source 17, one end of the glass tube 3 is a closed structure, and the other end is an open structure; the plurality of glass tubes 3 are horizontally evenly distributed on the side wall of the tank body 1, and the closed end of the glass tube 3 extends into the tank body 1, and the open end of the glass tube 3 is arranged outside the tank body 1; the glass tube 3 is connected to a threaded connection column, and is threadedly connected to the tank body 1 through the threaded connection column, and a sealing gasket is arranged at the connection between the threaded connection column and the tank body 1, and the above-mentioned threaded connection structure makes it easier to disassemble and maintain the glass tube 3.

[0026] The length and position of the glass tube 3 extending into the tank body 1 do not interfere with the stirring device, and the stirring paddle 16 is staggered with the position of the glass tube 3. This design of introducing the glass tube 3 can increase the stirring resistance, and can break up the water vortex during stirring, so that the liquid in the tank is easier to disperse. Another function of the glass tube 3 is to support the light source 17. The glass tube 3 is provided with a light source 17 and a heat dissipation structure; the light source 17 is evenly distributed in the tank body 1 from top to bottom, which can improve the uniformity of the divergent light inside the tank body 1. The heat dissipation structure includes a cold air pipe 6 and a cold air supply device, one end of the cold air pipe 6 is connected to the cold air supply device, and the other end extends into the glass tube 3. The cold air supply device blows low-temperature air into the glass tube 3 through the cold air pipe 6, which can effectively reduce the abnormal temperature rise in the tank body 1 caused by the heat of the light source 17, and can discharge the heat generated in the glass tube 3, so as to avoid product contamination or fermentation failure due to the heat rupture of the glass tube 3. The open end of the glass tube 3 is located outside the tank body 1, which is more convenient for controlling the light source 17 and the heat dissipation device.

[0027] The second light source system is arranged on the inner side of the top of the tank body 1; in this embodiment, the second light source system includes five incandescent lamps 7, which are evenly distributed on the inner side of the top of the tank body 1 with the axis of the tank body 1 as the center. The second light source system assists the first light source system to illuminate the inside of the tank body 1. If the glass tube 3 is affected by the cell wall growth, the incandescent lamp 7 on the top can be turned on to illuminate the inside of the tank body 1. This part of the light source is not affected by the increase in bacterial concentration and the limited light intensity caused by the bacteria attached to the glass tube wall.

[0028] An annular gas disperser 2 is provided at the bottom of the tank body 1; the annular gas disperser 2 is provided with a plurality of vents 4, and a one-way valve is provided on the vent 4. The annular gas disperser 2 is connected to a sterile gas supply device through a pipeline 5. The sterile oxygen provided by the sterile gas supply device is sent into the annular gas disperser 2 through the pipeline 5, and then enters the tank body 1 through the plurality of vents 4, so that the gas entering the tank body 1 is more dispersed, and the dissolved oxygen in each part is more uniform and stable, which is conducive to bacterial production.

[0029] In this embodiment, the annular gas disperser 2 is an annular tube, and the bottom of the annular gas disperser 2 is connected to the inner wall of the tank bottom of the tank body 1 through a bracket.

[0030] The method for using a photosynthetic bacteria preparation fermentation tank described in this embodiment is as follows:

[0031] 1. The first step is to use a dark aerobic fermentation method: using the annular gas disperser 2 arranged at the bottom of the tank body 1 in combination with a traditional paddle-type agitator to make the dissolved oxygen in the tank body 1 reach the level required for photosynthetic bacteria production for fermentation.

[0032] 2. The second step adopts the light anaerobic fermentation method: the sterile gas supply device is turned off, only the positive pressure of 0.01 MPa is kept in the tank body 1, the first light source system on the side wall of the tank body 1 and the second light source system on the top of the tank body 1 are turned on, and the fermentation is carried out by stirring at a low speed.

[0033] The utility model can realize a two-step fermentation process of photosynthetic bacteria. In the first step, the photosynthetic bacteria are rapidly fermented under dark aerobic conditions by utilizing the characteristics of fast growth, low energy consumption and simple process. Oxygen is provided to the tank through an annular gas disperser at the bottom of the tank body, and the first light source system and the second light source system are turned off at the same time to provide conditions for dark aerobic fermentation. The number of bacteria is rapidly increased at this stage. After the first step of fermentation is completed, the gas supply system is turned off, the first and second light source systems are turned on, and the second step of light anaerobic fermentation is started to promote the synthesis of photosynthetic bacteria pigments and complete the final fermentation.

[0034] The above content is a further detailed description of the utility model in combination with a specific preferred implementation method. It cannot be determined that the specific implementation methods of the utility model are limited to this. For ordinary technicians in the technical field to which the utility model belongs, they can make several simple deductions or substitutions without departing from the utility model, which should be regarded as belonging to the utility model and the scope of patent protection determined by the submitted claims.

Claims

1. A photosynthetic bacteria preparation fermentation tank, comprising a tank body (1), wherein a stirring device is provided inside the tank body (1); characterized in that: The tank body (1) is connected to a first light source system and a second light source system; the first light source system comprises a plurality of glass tubes (3), one end of the glass tubes (3) being a closed structure and the other end being an open structure; the plurality of glass tubes (3) are evenly distributed on the side wall of the tank body (1), and the closed end of the glass tube (3) extends into the tank body (1), while the open end of the glass tube (3) is arranged outside the tank body (1); a light source (17) and a heat dissipation structure are arranged in the glass tube (3); the length and position of the glass tube (3) extending into the tank body (1) do not interfere with the stirring device; the second light source system is arranged on the inner side of the top of the tank body (1); an annular gas disperser (2) is arranged at the bottom of the tank body (1); a plurality of vents (4) are arranged on the annular gas disperser (2), and a one-way valve is arranged on the vent (4); the annular gas disperser (2) is connected to a sterile gas supply device via a pipeline (5).

2. A photosynthetic bacteria preparation fermentation tank according to claim 1, characterized in that: The heat dissipation structure comprises a cold air pipe (6) and a cold air supply device; one end of the cold air pipe (6) is connected to the cold air supply device, and the other end extends into the glass tube (3).

3. A photosynthetic bacteria preparation fermentation tank according to claim 1, characterized in that: The annular gas disperser (2) is an annular tube body, and the bottom of the annular gas disperser (2) is connected to the inner wall of the tank bottom of the tank body (1) through a bracket.

4. The photosynthetic bacteria preparation fermentation tank according to claim 1, characterized in that: The second light source system comprises a plurality of incandescent lamps (7), and the plurality of incandescent lamps (7) are evenly distributed on the inner side of the top of the tank body (1) with the axis of the tank body (1) as the center.

5. A photosynthetic bacteria preparation fermentation tank according to claim 4, characterized in that: A manhole (8) is provided on the top of the tank body (1).

6. The photosynthetic bacteria preparation fermentation tank according to claim 1, characterized in that: The tank wall of the tank body (1) is a double-layer structure; a feed port (9) is provided at the upper portion of the side wall of the tank body (1); and a discharge port (10) is provided at the lower portion of the side wall of the tank body (1); control valves are provided on the feed port (9) and the discharge port (10).

7. The photosynthetic bacteria preparation fermentation tank according to claim 1, characterized in that: The tank body (1) is provided with a pH probe (11), a dissolved oxygen probe (12) and a temperature probe (13).

8. The photosynthetic bacteria preparation fermentation tank according to claim 1, characterized in that: The stirring device comprises a motor (14), a stirring shaft (15) and a stirring paddle (16); the motor (14) is arranged at the top of the tank body (1); the stirring shaft (15) is connected to the motor (14) and is vertically arranged inside the tank body (1); the stirring paddle (16) is evenly distributed on the stirring shaft (15) from top to bottom; the stirring paddle (16) and the glass tube (3) are arranged in an alternating manner.

Images